<?xml version="1.0" encoding="UTF-8"?>
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 xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance"
 xsi:schemaLocation="http://uniprot.org/uniprot http://www.uniprot.org/docs/uniprot.xsd">
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5UWT3</accession>
    <name>R5UWT3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nitroreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00588</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000008">
        <property type="protein sequence ID" value="CCZ82783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82783">
        <property type="protein sequence ID" value="CCZ82783"/>
        <property type="gene ID" value="BN566_00588"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.109.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029479">
        <property type="entry name" value="Nitroreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000415">
        <property type="entry name" value="Nitroreductase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00881">
        <property type="entry name" value="Nitroreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55469">
        <property type="entry name" value="SSF55469"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitroreductase" evidence="1">
        <location>
            <begin position="11"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="175" mass="18995" checksum="23107DA6D990534E" modified="2013-07-24" version="1">MILHNDAVDAILTRRSIRSYQAKQISEDALETILTAGQYAPSGMNQQSAKAIVVQSKERMQQLVQLAKAAEQVDHNPFYDAPTVILVFADATQLAPIEDGCNVLENMMLAAHAIGLGSCWIYAAKVLFSTEEGAAFQKELGLSETDQIVGSIAVGYPDGAAPQPKPRKESFFRIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXK8</accession>
    <name>R5UXK8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00615</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000009">
        <property type="protein sequence ID" value="CCZ82810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82810">
        <property type="protein sequence ID" value="CCZ82810"/>
        <property type="gene ID" value="BN566_00615"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="49"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="183" mass="21113" checksum="6664ED14540FCFAB" modified="2013-07-24" version="1">MKMTSAQAAKLLRQWNESLKALQRREENTKTFLASLGEDLESVRPEYDYAAMQAEQAALEAXXXQAEQAAIEANIRKLKHTLNVFNTTTIIPEFEMTIDQMLIYLPQLTNRLEKLSRMKELLPKQRENALYSRNSAIIDYRYANYDIQTVEKDYEVLAETLAKAQTALDYINNTVLLEVDLPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXN6</accession>
    <name>R5UXN6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00846</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000043">
        <property type="protein sequence ID" value="CCZ83053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00846"/>
    <dbReference type="EnsemblBacteria" id="CCZ83053">
        <property type="protein sequence ID" value="CCZ83053"/>
        <property type="gene ID" value="BN566_00846"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025945">
        <property type="entry name" value="DHHW"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14286">
        <property type="entry name" value="DHHW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="345" mass="39246" checksum="D1341BA2207FF582" modified="2013-07-24" version="1">MKKNRLSFWLLLPCFVGMMLLCGKNMLEHPLTASLKTSSQPTFVQKWYTALQMQLGNHQRNGVYIGETALYALPQPLSETALSNTADIINQFYQETELPICVTAIPDAASFYSDAFPDGMPYVEQKPAIKQFYNAIDLHIRKTDAYYILEAESNDYVYYRTFPYWTSYGAYSVYRSVIQKLGFVPISYDHYTVSHVKSDARGALYQATQANAVMPDLIDVYENNSNPLTCTVTTTLQDSSKKERDSLYDADALQTENPYQFYLGEPAPLQVVTTNVQNGKKLLVIKDEWADCMIPFLAQHYETIYLIDLHHSTTLPAAADYQQVLFLCHTDTYTETEAFANLLTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXT3</accession>
    <name>R5UXT3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Carbamoyl-phosphate synthase small chain</fullName>
            <ecNumber evidence="2">6.3.5.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Carbamoyl-phosphate synthetase glutamine chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">carA</name>
        <name evidence="4" type="ORF">BN566_00915</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>2 ATP + H2O + hydrogencarbonate + L-glutamine = 2 ADP + carbamoyl phosphate + 2 H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:18633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the CarA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.5" evidence="2"/>
    <dbReference type="EMBL" id="CBAR010000061">
        <property type="protein sequence ID" value="CCZ83128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00915"/>
    <dbReference type="EnsemblBacteria" id="CCZ83128">
        <property type="protein sequence ID" value="CCZ83128"/>
        <property type="gene ID" value="BN566_00915"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00171"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00115"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01744">
        <property type="entry name" value="GATase1_CPSase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01209">
        <property type="entry name" value="CPSase_S_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006274">
        <property type="entry name" value="CarbamoylP_synth_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002474">
        <property type="entry name" value="CarbamoylP_synth_ssu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036480">
        <property type="entry name" value="CarbP_synth_ssu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035686">
        <property type="entry name" value="CPSase_GATase1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00988">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01097">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52021">
        <property type="entry name" value="SSF52021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01368">
        <property type="entry name" value="CPSaseIIsmall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CPSase_sm_chain" evidence="3">
        <location>
            <begin position="8"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="CPSase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="257"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="342"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="368" mass="40562" checksum="FC29F9F512278E86" modified="2013-07-24" version="1">MSLFNQGEKAYLLLADGTILEGIHFGVKGTAIGEVVFTTGMTAYQETMTDPSYYGQLVIQTFPLIGNYGVNEADNESDRSYIRGYIVREWCNTPSNFRMEGDVNSFLIEQNIIGIHSIDTRSLTRKIREYGVMNGAITTEDVYAQKDALLEQIRAYTVKNAICSVTGDKKRVYSNPNPKYRVALFDFGYKRNIRNELVARGCEVTVLPALTSAEEVKAMAPDGIMLSNGPGDPTENQAVIDNVRAVAELGIPIFGICLGHQLLALSQGARTEKLKYGHRGANQPVIDLQSGRTYVTSQNHGYAVVGDSLDPSIGTVSHVNANDHTCEGIRYHKINAFTVQFHPEAHGGPLDTAYLFDEFLAGMEKEKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXW1</accession>
    <name>R5UXW1_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4097 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00101</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000024">
        <property type="protein sequence ID" value="CCZ82910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82910">
        <property type="protein sequence ID" value="CCZ82910"/>
        <property type="gene ID" value="BN566_00101"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025164">
        <property type="entry name" value="DUF4097"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13349">
        <property type="entry name" value="DUF4097"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004406548" description="DUF4097 domain-containing protein" evidence="1">
        <location>
            <begin position="35"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4097" evidence="2">
        <location>
            <begin position="55"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ82910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="319" mass="34565" checksum="321D622F22DB6779" modified="2013-07-24" version="1">MKPFSKCYYLNACGLLLAGLIVTGISVGAGAWNAEKYGEALNLKDRYFEVTEPFTSMQLISNTANVVVKATDTDTCTVTATNMPENTIKVTVEDDKLCINAVPEHRKWYQYFQMGVNPPTSTITLSLPMNVYEQVELQNDIGNLTLQNVHADRISLKSDTGDMDLQSITGKSCSITNNIGSLSLQEVQIREQTDIQQDTGDLELTDCTFHGSSAISTQIGSMHLSDSEFAATAITSDTGDIRLKDCTFYQVCTIKSDVGDVKGSLLADVNNTSIAADTDVGSVSIDGKKNGYQVPNALNTITIRVDTGDIRLTTQQPTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5UY06</accession>
    <name>R5UY06_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00991</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000072">
        <property type="protein sequence ID" value="CCZ83208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83208">
        <property type="protein sequence ID" value="CCZ83208"/>
        <property type="gene ID" value="BN566_00991"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="33" mass="3843" checksum="EE7A65EF273C3E70" modified="2013-07-24" version="1">MTKRELRKKLNALNIDKFAYDLNGGLPNETYCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5UY58</accession>
    <name>R5UY58_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01044</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83263">
        <property type="protein sequence ID" value="CCZ83263"/>
        <property type="gene ID" value="BN566_01044"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="31" mass="3530" checksum="2AD3EE6350064330" modified="2013-07-24" version="1">MKIVVVKTPKLLTGIFRLVFKVKRAEPESDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYH7</accession>
    <name>R5UYH7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00868</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000051">
        <property type="protein sequence ID" value="CCZ83080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00868"/>
    <dbReference type="EnsemblBacteria" id="CCZ83080">
        <property type="protein sequence ID" value="CCZ83080"/>
        <property type="gene ID" value="BN566_00868"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002577">
        <property type="entry name" value="HTH_HxlR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01638">
        <property type="entry name" value="HxlR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51118">
        <property type="entry name" value="HTH_HXLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH hxlR-type" evidence="1">
        <location>
            <begin position="12"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="124" mass="14226" checksum="BE02A143658883A2" modified="2013-07-24" version="1">MYQPKMEKDIRCPLEYGLATFGGKWKSRIICVLAAQAPLRYSVLRKEMGNITDAVLSSTLKELIADGMVLRTSYDEIPPRVEYQLTEKGTSVVPILQQICQWAGAYHKEEQIHPMIQCEKCDYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYM3</accession>
    <name>R5UYM3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cna protein B-type domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01189</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000098">
        <property type="protein sequence ID" value="CCZ83413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01189"/>
    <dbReference type="EnsemblBacteria" id="CCZ83413">
        <property type="protein sequence ID" value="CCZ83413"/>
        <property type="gene ID" value="BN566_01189"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041033">
        <property type="entry name" value="Prealbumin-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17802">
        <property type="entry name" value="SpaA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="1" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="1" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SpaA" evidence="3">
        <location>
            <begin position="64"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="SpaA" evidence="3">
        <location>
            <begin position="159"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="254"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="254"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="387" mass="41322" checksum="73E1BD4AA850433B" modified="2013-07-24" version="1">MTTTEEVTTTEEETTTEEVTTEETTTETTIPERTITIHKIAPAAASATQSEDGIYTIQYDPHDMAGVTFGIYDADGNLVETVITDENGYAESSILNGDYYTVQEMQTVSGYVMSTEPYVIDFTTNEGDTANLEITNDIIRCQIHLSKQLEGNAGDITMVQFGLYTAQDIPLLDGTVVPSDSCIGIANAQEDGTILFEDTLPAGNYYVKELATADGYVLSDTIYPVEIIYPEDQTIADITFEVNNGDPIINYQTTTTTTTTEEVTETQPEETTVTTVTETTASTGTSDVHGGTDSKTTTSTTTTTTTTTTTTTTTITTTKDDSSSTTKKKTTSSSGSGKTSSGGSSSGSTKTTPKTADDRMLPIVITGSAAFLFAAASKPKRKKNQKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYN9</accession>
    <name>R5UYN9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mannose-6-phosphate isomerase</fullName>
            <ecNumber evidence="3">5.3.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_01202</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-mannose 6-phosphate = D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:12356"/>
            <dbReference type="ChEBI" id="CHEBI:58735"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="EC" id="5.3.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="4">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mannose-6-phosphate isomerase type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.8" evidence="3"/>
    <dbReference type="EMBL" id="CBAR010000102">
        <property type="protein sequence ID" value="CCZ83428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01202"/>
    <dbReference type="EnsemblBacteria" id="CCZ83428">
        <property type="protein sequence ID" value="CCZ83428"/>
        <property type="gene ID" value="BN566_01202"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004476">
        <property type="term" value="F:mannose-6-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001250">
        <property type="entry name" value="Man6P_Isoase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014628">
        <property type="entry name" value="Man6P_isomerase_Firm_short"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01238">
        <property type="entry name" value="PMI_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036894">
        <property type="entry name" value="PMI_Firm_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="active site" evidence="5">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR036894-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR036894-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ83428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="326" mass="35872" checksum="C2F7639D665BD740" modified="2013-07-24" version="1">MKHVPFLLRPVGKDYLWGGERLKTEYHKQIDLTPLAETWECSTHPDGCSVVDSGAAAGKPLRAVLQEHPEYLGIYANSEGELPILIKLIDAKKDLSVQVHPDDAYAKQYENGQNGKTEMWYVLEAEPDASLVYGFRHTMTPERLRQSLAEGTLEADLQSVPVSKNDAFLIPAGTVHAIGAGILLAEIQQSSNLTYRLYDYNRTDANGNKRPLHIEKAVATVDYQQKPLPEQNRTVEQKDGYTEELLCACPYFQVFRLHLQAATPAVAAAVSTTNAFAVLLCIFGSGTMTAETGETLAFQKGQCVFVPADTTVKELSGTADLLWITA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYQ7</accession>
    <name>R5UYQ7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00953</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000069">
        <property type="protein sequence ID" value="CCZ83170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83170">
        <property type="protein sequence ID" value="CCZ83170"/>
        <property type="gene ID" value="BN566_00953"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="86" mass="8698" checksum="177DE5BC1CB8309F" modified="2013-07-24" version="1">MQWSPVPKAELPAYLKKAAALLAVATVLGGCSGQPSDSEPTQTALAGEVETTAPTTTTTTSTTSELLQLAGEIDISESDAVTSSDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYR2</accession>
    <name>R5UYR2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00958</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000069">
        <property type="protein sequence ID" value="CCZ83175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83175">
        <property type="protein sequence ID" value="CCZ83175"/>
        <property type="gene ID" value="BN566_00958"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="76" mass="8631" checksum="54C387B475B01C77" modified="2013-07-24" version="1">MMLECQVGMQSDTQARKAQAILQRYGYSCRIQRQVHATPEGCSYLLQTKGTCKPIADLLKENDIPFLSLRNVRDAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYW1</accession>
    <name>R5UYW1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01262</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000117">
        <property type="protein sequence ID" value="CCZ83493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01262"/>
    <dbReference type="EnsemblBacteria" id="CCZ83493">
        <property type="protein sequence ID" value="CCZ83493"/>
        <property type="gene ID" value="BN566_01262"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="247"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="401"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="432"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="457"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="528"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="551"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="580"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="662"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="699"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="729"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="766"/>
            <end position="786"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="810" mass="91245" checksum="9C9633A4209FED26" modified="2013-07-24" version="1">MILYLLFLIFCNLICYVIAYFVRGDEEKPIPSHWVKCGITTVLEIVLLFILRGIATAFFDADNQPFAVAPLLVTLLLIGLGTAFSRRSTTSNLRNYLRKAAWIAGVVFVLESFVFHYTSFTTHPETTDLVLSQAEINDASAAQIVDDTIQISGNCTLTFSNLEQNPDFRYLILNIDGEDCYYNVSCSIEDDNLSNRFEQAGKSQGTGTYSSIRFSMLPYQTLHTVQLQFTDVNAALVLHNGTFHTAVPYQFSAARFFLIVIIALVLTACKQFRIWNIRYRAHSWKHNLAVLVTLFGCMASVLAFRVPELEPLPISEPIDVNNVYAMTLDAWEKGQTNMDLTPSEELLQSETPYDNSNREGVYYNWDYAYYNQKYYCYFGCAPVALLYVPYYKLTGNVLPLNWAYCIMAEAVIVTLFGLILTLVRKFCKRPPLILLLLGLVSAVAGSGVLFGLNYSDRYHLCILTGMFGLFLSLWMGFAAMDSSYAVRTTKQLRKLSLQRAKKDNGEHLEQEEIPMPVSPYTVKKRENWKRFVLLAVSAIGTVITAASRPNMLLYVLILVPVFLHLLFRKDLKLSAKITSAACYLIPVAAGAAAIMWYNKIRFDDPFQFGSIYQMTVDNTAANKITLSRLPAAIAVYFFGGLDQLNDFPFLRTKYSNIANRQMYLYVEGTIGAFTLPSVLLGALSFPFVWKRWKRKHSGFVRRVILAMIAVLAVALAWVDFSMAGVTLRYALDILVILSVLSTILLLQVPSLLKTYHASLARVSEKVIAAAMVGTVVVCLCILIASGESVSLFKAHPTIWNTWKEIFVFWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZ16</accession>
    <name>R5UZ16_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01076</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01076"/>
    <dbReference type="EnsemblBacteria" id="CCZ83295">
        <property type="protein sequence ID" value="CCZ83295"/>
        <property type="gene ID" value="BN566_01076"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="4"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="128" mass="13994" checksum="53ACA9C5EA9A3B7B" modified="2013-07-24" version="1">MIQGMHHVSMKCSNAEEYAAVRAFYTELLRIPVLTEWETGILLDTGAGIVEIFQDETAEKLGQGVIRHFAFAVKDVEACVAAVRAGGYPILVEPKTVAIGGNPAFSAKIAFCKGALGEEIEFFQQMWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZ85</accession>
    <name>R5UZ85_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">T5orf172 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01379</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000147">
        <property type="protein sequence ID" value="CCZ83643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01379"/>
    <dbReference type="EnsemblBacteria" id="CCZ83643">
        <property type="protein sequence ID" value="CCZ83643"/>
        <property type="gene ID" value="BN566_01379"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025280">
        <property type="entry name" value="DUF4041"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018306">
        <property type="entry name" value="Phage_T5_Orf172_DNA-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13250">
        <property type="entry name" value="DUF4041"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00974">
        <property type="entry name" value="T5orf172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="39"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="T5orf172" evidence="3">
        <location>
            <begin position="388"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="96"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="294"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="501" mass="58364" checksum="9FAFFA1C00946525" modified="2013-07-24" version="1">MKYCLHCKTLNENTQTHCTHCGKRLSNSKRITPIYCDPIVIAICFALWMFVIPLAAGVYLLMKQKSAYKENMNEYENALLFYGVKMGESGQINNANSKANEIISRANQEAQTIEQESEKTQKKAEQALQKLKEDQGKLQNNLAELQAEQEKERNYLEELQSEITYELSKVDVDENVTSEEYKNRLKIMQLEEKNIVTNNEAVEIIQIYDSKAQKADIKQILRCFNSECTTIIVNTTVKNMDTSRNKMMRAFEIINKIFVTDGIAITQKLLENKMAQLTAKYQYEYQREQERLQQKAIREQMVEEEKARREIEKEKEKLTKEENQFKKEIDKLMKYMQNTQDVEKQLYISQIEELQKKLADVQKSKEDVLHKEQNTRAGFVYVISNIGSFGENVYKIGMTRRLEPMDRISELSSASVPFEFDVHAMIFSDDAPALEAILHSTFADKRVNLVNTRKEFFRVSLDEIEAVVKKNYNATVTFTKTAEAYQYRESQRLREQGGNIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZB5</accession>
    <name>R5UZB5_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">Signal peptidase I</fullName>
            <ecNumber evidence="4 7">3.4.21.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN566_01419</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.</text>
            <dbReference type="EC" id="3.4.21.89"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Membrane</location>
            <topology evidence="8">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the peptidase S26 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.89" evidence="4 7"/>
    <dbReference type="EMBL" id="CBAR010000153">
        <property type="protein sequence ID" value="CCZ83683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01419"/>
    <dbReference type="EnsemblBacteria" id="CCZ83683">
        <property type="protein sequence ID" value="CCZ83683"/>
        <property type="gene ID" value="BN566_01419"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000223">
        <property type="entry name" value="Pept_S26A_signal_pept_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019758">
        <property type="entry name" value="Pept_S26A_signal_pept_1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019757">
        <property type="entry name" value="Pept_S26A_signal_pept_1_Lys-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019756">
        <property type="entry name" value="Pept_S26A_signal_pept_1_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43390">
        <property type="entry name" value="PTHR43390"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10502">
        <property type="entry name" value="Peptidase_S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00727">
        <property type="entry name" value="LEADERPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="SSF51306"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02227">
        <property type="entry name" value="sigpep_I_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00501">
        <property type="entry name" value="SPASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00760">
        <property type="entry name" value="SPASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00761">
        <property type="entry name" value="SPASE_I_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 7" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S26" evidence="9">
        <location>
            <begin position="23"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR600223-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF10502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ83683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="209" mass="23357" checksum="A74EB36D1D4B5C74" modified="2013-07-24" version="1">MEPNTEETTKKEEKGTFWKDAADMLEAVLISIFCVIMLFAYVLRPVTVEGHSMESTLQDQDKLFMTDLFYTPERGDIVIIDNDASHTFDENGNLVEGQGLSTVTTEKRLIKRIIAVGGETLDINFDDGTVTIDGTVLDEPYINNLTLNDEGAFSYPITIPDGYYFVMGDNRQHSSDSRYPMVGLVAKDQIMGKAILRFAPISKFGGIYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZM6</accession>
    <name>R5UZM6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted Zn-dependent peptidases</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01543</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000164">
        <property type="protein sequence ID" value="CCZ83808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01543"/>
    <dbReference type="MEROPS" id="M16.A20"/>
    <dbReference type="EnsemblBacteria" id="CCZ83808">
        <property type="protein sequence ID" value="CCZ83808"/>
        <property type="gene ID" value="BN566_01543"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="SSF63411"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_M16" evidence="1">
        <location>
            <begin position="68"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M16_C" evidence="2">
        <location>
            <begin position="187"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="428" mass="48211" checksum="675B4EECA275CFA7" modified="2013-07-24" version="1">MAETKIERISCSQIGEQYTHIQHKSGLSIYVSEMEGFSTTEALFGTKYGSINTQFKTKQEKDFCCVPEGIAHYLEHKLFENEDCDVFDLYAKTGANANAFTSFDKTCYLFSCSSHYQESLEILLSFVQSPYFTKESVQKEQGIIGQEIQMCNDDPGWRIFFNTLQGLFHHHPVKIDIAGTIESIAQITPELLYRCYHTFYNLHNMVLSVAGNCKVDEVVALADKLLKPCEDMQLECIFPEEPMEIVRAETVETAPIGTTMFVLGYKCKPASGLENLKKELLATLALKVLTHVTSPLYQGMLKDGLINETFSTEIFNGDDFFVPMLEGESKDPYAVRERIFAAVEEAAKNGLDETLFENSRKNAYGSLIMKFNNVEAVANLMINSAMSGIAPFDTVTMLSSLTIEDANRWLREELLPQQATLSIMKNQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZX9</accession>
    <name>R5UZX9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">UDP-N-acetylglucosamine 1-carboxyvinyltransferase</fullName>
            <ecNumber evidence="7">2.5.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Enoylpyruvate transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">UDP-N-acetylglucosamine enolpyruvyl transferase</fullName>
            <shortName evidence="7">EPT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">murA</name>
        <name evidence="9" type="ORF">BN566_01637</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine</text>
            <dbReference type="Rhea" id="RHEA:18681"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:68483"/>
            <dbReference type="EC" id="2.5.1.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the EPSP synthase family. MurA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.7" evidence="7"/>
    <dbReference type="EMBL" id="CBAR010000172">
        <property type="protein sequence ID" value="CCZ83908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01637"/>
    <dbReference type="EnsemblBacteria" id="CCZ83908">
        <property type="protein sequence ID" value="CCZ83908"/>
        <property type="gene ID" value="BN566_01637"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008760">
        <property type="term" value="F:UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019277">
        <property type="term" value="P:UDP-N-acetylgalactosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01555">
        <property type="entry name" value="UdpNAET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00111">
        <property type="entry name" value="MurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001986">
        <property type="entry name" value="Enolpyruvate_Tfrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036968">
        <property type="entry name" value="Enolpyruvate_Tfrase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005750">
        <property type="entry name" value="UDP_GlcNAc_COvinyl_MurA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00275">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="SSF55205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01072">
        <property type="entry name" value="murA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1 7" id="KW-0132">Cell division</keyword>
    <keyword evidence="3 7" id="KW-0133">Cell shape</keyword>
    <keyword evidence="6 7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="7" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="EPSP_synthase" evidence="8">
        <location>
            <begin position="8"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphoenolpyruvate binding" evidence="7">
        <location>
            <begin position="22"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="2-(S-cysteinyl)pyruvic acid O-phosphothioketal" evidence="7">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ83908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="420" mass="44715" checksum="60C2F319A49A8471" modified="2013-07-24" version="1">MQKLVITGGIPLEGTVTLQGAKNSVLPILAASLLCRGETVLENCPRLTDVFSACRILTALGCRCSMKGHVARIQADGVDCVEISESLMQEMRSSIIFLGALLGRYGSCTLSYPGGCELGPRPIDMHLQALRSMGAEIVQNGGRLLCRAAKGLHGEKIPFKYPSVGATENIMLAAVLAKGTTVIYNAAREPEICDLASFLRQCGANIYGDGGDTITIEGVSALHGTVYRIMPDRIVGMTYLTAAAMTGGTLQMKQTEPSQMENLLPVLEQAGCRLDVAQKTIYLHAPARLRALPPIRTMPHPGFPTDAQAIVMAMLTGADGVSVFEETVFENRYRHVDALLKMGAEIYVSGQTAVVKGVRQLSGAPVRATDLRGGAAMVLAGLAAQGVTEVTQIFHIDRGYEAIETVLSGIGASIQREILE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5V022</accession>
    <name>R5V022_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01672</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000174">
        <property type="protein sequence ID" value="CCZ83943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01672"/>
    <dbReference type="EnsemblBacteria" id="CCZ83943">
        <property type="protein sequence ID" value="CCZ83943"/>
        <property type="gene ID" value="BN566_01672"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="252"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="290"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="321"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="352"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="708" mass="80165" checksum="52400EDFA6883D3B" modified="2013-07-24" version="1">MREINKKPFSVGLYIEGLRQLRIVGILGAILLFCSSAIITISNALFTQRQMELYQDSSIVLANLSALEVAPLLLLLFSVFAPVMTLWLFHFMNKRNSSDFLFAIPQTRMALYCSYTSSIVTWLAILALSGLIGVLIPAAIFSTYINIPYHALFLLTLSCFLYSLQVMAAILVAMSITGTMFSNIILSALILFAPRLLIFMLTGIWKNIPFIYASSDHFSLLNDSINPISGCVLYPLCGISDTYQLFSSAAPMIYSVCLSVIYLLLAAFLFQKRKSETAGKSASSPILQSIYRITVTMIYCVVVAAVILSEANAMQDATGRFEIIVAYLVAVAIYFLFELLTTKSVQKMWKAAPGLLVVLVLNGVMIFGMHSIIQRAYNFQPTAEEVTGITFLPQSTNNGNQISYDDYVQSHYMGTITDKDLIAAILDNLKSDVQDMKEGGSQQYYNDEKRYAYAEFNFKIITKTGTRYRHIRIDPKTQRALAEQTYESMKDDSSWRNPPEPIEQTLWTYDRNGSYGGMNERNASQLFETLKTELQTVDFSAWYTANYSTETAVFWFDYDIYDNNSNYQISIPVYASIYPKTVEQYQTYLYEQQQEDLSKLKDFVKNDMPKLQFAEDYDISLSIHISGETAIEYPYSSPENTNLLSEILEKGLLDHPITDTDSYVELDVDVYDDEYTNIYDDDRDFYGGNFCIAADTDFLKSLLQPSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5V091</accession>
    <name>R5V091_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phage related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01411</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000148">
        <property type="protein sequence ID" value="CCZ83675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83675">
        <property type="protein sequence ID" value="CCZ83675"/>
        <property type="gene ID" value="BN566_01411"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="94" mass="10860" checksum="F0F94BB0D5D6B6E3" modified="2013-07-24" version="1">MNVDLLTMLKVDLGITAEAYNDRLYADLQAAKSYIAREGITLNETIEDDQLVVQYAAWLWRKRGGDEQSSMPRMLRYLLNNRLFSEKMRGNDDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0A9</accession>
    <name>R5V0A9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">4-diphosphocytidyl-2-C-methyl-D-erythritol kinase</fullName>
            <shortName evidence="9">CMK</shortName>
            <ecNumber evidence="2 9">2.7.1.148</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ispE</name>
        <name evidence="12" type="ORF">BN566_01436</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>4-CDP-2-C-methyl-D-erythritol + ATP = 4-CDP-2-C-methyl-D-erythritol 2-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57823"/>
            <dbReference type="ChEBI" id="CHEBI:57919"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.148"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="9">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the GHMP kinase family. IspE subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.148" evidence="2 9"/>
    <dbReference type="EMBL" id="CBAR010000154">
        <property type="protein sequence ID" value="CCZ83700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01436"/>
    <dbReference type="EnsemblBacteria" id="CCZ83700">
        <property type="protein sequence ID" value="CCZ83700"/>
        <property type="gene ID" value="BN566_01436"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00056">
        <property type="reaction ID" value="UER00094"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050515">
        <property type="term" value="F:4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019288">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00061">
        <property type="entry name" value="IspE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004424">
        <property type="entry name" value="IspE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43527:SF2">
        <property type="entry name" value="PTHR43527:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010376">
        <property type="entry name" value="IspE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="SSF55060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00154">
        <property type="entry name" value="ispE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="6 9 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP_kinases_N" evidence="10">
        <location>
            <begin position="91"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP_kinases_C" evidence="11">
        <location>
            <begin position="227"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="11"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="141"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="99"/>
            <end position="109"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CCZ83700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="294" mass="31603" checksum="657CDAF53F9EFD2A" modified="2013-07-24" version="1">MKRMVLKTPAKINLALDVVGKRADGYHTLETVFQTVSLYDTITVTLFPHRTGEQAIRILCNIPWIPKDERNIAWKAVDLFLKKTGCPVGFDIYLQKYIPSQAGLGGGSSDAAAVLYACNQLTGAGLSHAELCEMGLQLGADVPFFFYGGTAYAAGIGEEIQPLPYLGDVSVVLAKGKGGISTAAAYAAMDALQNPPHPNVPEMKQRLQAYAEPKDLAPLCGNLFAEVTELPIVQKLQQEMLARGAFASLMTGSGSAVFGLFSSQEPARKCCSALRKVVPFVAQCKTIPHTFYAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0D7</accession>
    <name>R5V0D7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">tRNA-specific 2-thiouridylase MnmA</fullName>
            <ecNumber evidence="7">2.8.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">mnmA</name>
        <name evidence="8" type="ORF">BN566_01470</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>AH2 + ATP + S-sulfanyl-L-cysteinyl-[protein] + uridine(34) in tRNA = 2-thiouridine(34) in tRNA + A + AMP + diphosphate + H(+) + L-cysteinyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:47032"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11726"/>
            <dbReference type="Rhea" id="RHEA-COMP:11727"/>
            <dbReference type="Rhea" id="RHEA-COMP:11728"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61963"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:87170"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.8.1.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the MnmA/TRMU family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.13" evidence="7"/>
    <dbReference type="EMBL" id="CBAR010000159">
        <property type="protein sequence ID" value="CCZ83735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01470"/>
    <dbReference type="EnsemblBacteria" id="CCZ83735">
        <property type="protein sequence ID" value="CCZ83735"/>
        <property type="gene ID" value="BN566_01470"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016783">
        <property type="term" value="F:sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01998">
        <property type="entry name" value="tRNA_Me_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00144">
        <property type="entry name" value="tRNA_thiouridyl_MnmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023382">
        <property type="entry name" value="Adenine_a_hdrlase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004506">
        <property type="entry name" value="tRNA-specific_2-thiouridylase"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00420">
        <property type="entry name" value="trmU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="2 7" id="KW-0820">tRNA-binding</keyword>
    <feature type="region of interest" description="Interaction with tRNA" evidence="7">
        <location>
            <begin position="150"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with tRNA" evidence="7">
        <location>
            <begin position="308"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="102"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine persulfide intermediate" evidence="7">
        <location>
            <position position="201"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="8"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with tRNA" evidence="7">
        <location>
            <position position="127"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with tRNA" evidence="7">
        <location>
            <position position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="361" mass="39895" checksum="48754A7146396E27" modified="2013-07-24" version="1">MQKKVLVAMSGGVDSSATAAILCQKGYDCIGVTMKLYNLPDDAVRSNTCCTLSDAEDAKSVAYRLGMRYYVMNFTADFSKEVIDRFVSAYERGETPNPCIDCNRYMKFEQLYQRAALLGCDAIATGHYARIAYDEERGRWLLKKSKNEAKDQSYVLYSMTQEQLAHTIFPLGDYTDKAEVRKLAAESGLLNAEKQDSQDICFVPDGDYAAFIEQYTGKQYPHGDFVDRNGKKLGEHKGMIGYTIGQRKGLGISAPHPLYVCKKCVEDNTIVLSEREGLYTTKLIARDFNWIAYDGAPKTPISVTARTRYHAKEAAATVTPLEDGTVQVVFAEPQRAVTAGQAVVLYDGEIVVGGGTITSAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0E1</accession>
    <name>R5V0E1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative indolepyruvate ferredoxin oxidoreductase beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01475</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000160">
        <property type="protein sequence ID" value="CCZ83740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01475"/>
    <dbReference type="EnsemblBacteria" id="CCZ83740">
        <property type="protein sequence ID" value="CCZ83740"/>
        <property type="gene ID" value="BN566_01475"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016903">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019752">
        <property type="entry name" value="Pyrv/ketoisovalerate_OxRed_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01558">
        <property type="entry name" value="POR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="SSF53323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="POR" evidence="2">
        <location>
            <begin position="11"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="190" mass="20469" checksum="85C081272B0754C6" modified="2013-07-24" version="1">MTKQIMIVGVGGQGTLLASRILGNAVTDAGYDVKVSEVHGMSQRGGSVVTYVKYGDAVYSPIVDRGTADLILAFEPLEAYRALPYLKQGGKMIANTQQIDPMPVIIGAMAYPEHICEKLDAVCDLTAVDALQLAVQAGSQKCVNVVLIGVMAKSTEIPYEKWLETIRNTVPAKFLDMNLKAFELGYQAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0K1</accession>
    <name>R5V0K1_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">6-carboxy-5,6,7,8-tetrahydropterin synthase</fullName>
            <ecNumber evidence="8">4.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN566_01844</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>7,8-dihydroneopterin 3'-triphosphate + H2O = 6-carboxy-5,6,7,8-tetrahydropterin + acetaldehyde + 2 H(+) + triphosphate</text>
            <dbReference type="Rhea" id="RHEA:27966"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18036"/>
            <dbReference type="ChEBI" id="CHEBI:58462"/>
            <dbReference type="ChEBI" id="CHEBI:61032"/>
            <dbReference type="EC" id="4.1.2.50"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8 10">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8 10">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the PTPS family. QueD subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.-.-.-" evidence="8"/>
    <dbReference type="EMBL" id="CBAR010000187">
        <property type="protein sequence ID" value="CCZ84118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01844"/>
    <dbReference type="EnsemblBacteria" id="CCZ84118">
        <property type="protein sequence ID" value="CCZ84118"/>
        <property type="gene ID" value="BN566_01844"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00391"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070497">
        <property type="term" value="F:6-carboxy-5,6,7,8-tetrahydropterin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008616">
        <property type="term" value="P:queuosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007115">
        <property type="entry name" value="6-PTP_synth/QueD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038418">
        <property type="entry name" value="6-PTP_synth/QueD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12589">
        <property type="entry name" value="PTHR12589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01242">
        <property type="entry name" value="PTPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006113">
        <property type="entry name" value="PTP_synth"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03367">
        <property type="entry name" value="queuosine_QueD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 8 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0671">Queuosine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 10" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="23"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="9">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="9">
        <location>
            <position position="129"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR006113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR006113-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR006113-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ84118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="143" mass="16424" checksum="4FF7AFE1D3F16A59" modified="2013-07-24" version="1">MYQLKAKAAFDSAHFLSGYKGKCANLHGHRWTIEVIVEQEDLQTEGVCRGMLIDFGDLKKALRALADFYDHALIYEEGSLRQTTVQALQEESFRLIPVPFRPTAECFAKHFFHQLQQQNLPVKRVIVYETPDNCAAYEEAETK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0L2</accession>
    <name>R5V0L2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-type sugar transport system permease component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_01540</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000164">
        <property type="protein sequence ID" value="CCZ83805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01540"/>
    <dbReference type="EnsemblBacteria" id="CCZ83805">
        <property type="protein sequence ID" value="CCZ83805"/>
        <property type="gene ID" value="BN566_01540"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008643">
        <property type="term" value="P:carbohydrate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="120"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="151"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="259"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="83"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ83805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="291" mass="32030" checksum="26A8447C9D55C388" modified="2013-07-24" version="1">MEQVGVKDNYRLVQNLKSAGCFIVCILLTLVCLVPLYLMIVNACRGHNAILQSPVSLIPGSNFVENMKNITTQSPQWNLFRGYRNSLIIAAGSTILTVFFSAMTAYGLTVYQFKVRDGAYTLILAVMMIPIQVTSTGFVQFMVGTLGLADSFIPLIVPAIAAPAIVFYMRQYMKSSFPLDIVEAARIDGCSEFRTFISIAIPMMKPAIAVQAIFAFVQNWNNYYTQNMILISNDKMITMPMMVQKVLGQDKKIDLGAQYAAVALSVIPIIVIYVCLSRFIVGGVALGGVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0M6</accession>
    <name>R5V0M6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Hemolysins and related proteins containing CBS domains</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN566_01555</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0053 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000165">
        <property type="protein sequence ID" value="CCZ83820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01555"/>
    <dbReference type="EnsemblBacteria" id="CCZ83820">
        <property type="protein sequence ID" value="CCZ83820"/>
        <property type="gene ID" value="BN566_01555"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04590">
        <property type="entry name" value="CBS_pair_CorC_HlyC_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002550">
        <property type="entry name" value="CNNM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044751">
        <property type="entry name" value="Ion_transp-like_CBS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005170">
        <property type="entry name" value="Transptr-assoc_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03471">
        <property type="entry name" value="CorC_HlyC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01595">
        <property type="entry name" value="DUF21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01091">
        <property type="entry name" value="CorC_HlyC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="SSF56176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51846">
        <property type="entry name" value="CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0129">CBS domain</keyword>
    <keyword evidence="7 9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004406638" evidence="11">
        <location>
            <begin position="24"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="80"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="CNNM transmembrane" evidence="13">
        <location>
            <begin position="1"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="12">
        <location>
            <begin position="195"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="12">
        <location>
            <begin position="258"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CCZ83820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="415" mass="45598" checksum="42D0D99A543A22DF" modified="2013-07-24" version="1">MIVIIIICIVLSALFSSSETAFASVNKIRLKSMADQGDKRALKALHIAEHYEDSLTAILVGNNIVNIGASSLATVVFTQWVGSAGPAVSTVAMTILVLIFGEVLPKSYAKSHAEKMALSVASALSAFTVVMTPFVKIFDVLSRALKPKTEAPSVTEDELKYIIDEIEEEGVLEEQESDLVLSALQFDETTVNDILIPRVKIVGVSSEATTDEICEMFLHSHFSRFPVYEKSMDNIIGMITSRDFFRLEHGMFHSVSEILQDVIYIPATKAISDSLKEMQRSKTHMAVVVDQYGGTKGIVTLEDIIEELVGDIYDESDEIVHEFVRTAPHCYTIVGTFSISDMRDALDEEDIPSQMPEAASTSVGGWVTELLGHIPEVGETVQVGTFQFQVLETDNQSVTKIALQVSPEQKEEPKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0P7</accession>
    <name>R5V0P7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aspartate--tRNA ligase</fullName>
            <ecNumber evidence="7">6.1.1.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Aspartyl-tRNA synthetase</fullName>
            <shortName evidence="7">AspRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">aspS</name>
        <name evidence="9" type="ORF">BN566_01575</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the attachment of L-aspartate to tRNA(Asp) in a two-step reaction: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp)</text>
            <dbReference type="Rhea" id="RHEA:19649"/>
            <dbReference type="Rhea" id="RHEA-COMP:9660"/>
            <dbReference type="Rhea" id="RHEA-COMP:9678"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the class-II aminoacyl-tRNA synthetase family. Type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.12" evidence="7"/>
    <dbReference type="EMBL" id="CBAR010000166">
        <property type="protein sequence ID" value="CCZ83840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01575"/>
    <dbReference type="EnsemblBacteria" id="CCZ83840">
        <property type="protein sequence ID" value="CCZ83840"/>
        <property type="gene ID" value="BN566_01575"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004815">
        <property type="term" value="F:aspartate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006422">
        <property type="term" value="P:aspartyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00044">
        <property type="entry name" value="Asp_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004524">
        <property type="entry name" value="Asp-tRNA-ligase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004115">
        <property type="entry name" value="GAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029351">
        <property type="entry name" value="GAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594:SF5">
        <property type="entry name" value="PTHR22594:SF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02938">
        <property type="entry name" value="GAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55261">
        <property type="entry name" value="SSF55261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00459">
        <property type="entry name" value="aspS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 7 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II" evidence="8">
        <location>
            <begin position="146"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Aspartate" evidence="7">
        <location>
            <begin position="201"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="223"/>
            <end position="225"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="451"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="485"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="492"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="537"/>
            <end position="540"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ83840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="596" mass="67397" checksum="39767593BC8EB3C4" modified="2013-07-24" version="1">MDTMQNLRRTCYCGEVTEAGKTVVVGGFVQKVRNLGNLIFIDLRDRTGIVQLAFNDETDREIFEKAAGCHSEYVLLAKGEVIPRASVNTDMKTGAVEIKVTELRVLSKAQTPPFTISDETKTNEELRLKYRYLDLRRAPLQRNLIMRHKIAQAAREYYYANDFIEIETPMMMKSTPEGARDYLIPSRVHNGKFYALPQSPQIYKQLLMVAGMDRYIQIARCFRDEDLRADRQPEFTQIDLEMSFVDVEDILQMTEGFVQYLFKKVLNMDIPTPLPRMTYKEAMERYGSDKPDTRFGMELQNISDLVSDLDFVVFRSAIEAGGSVRAIVAKQAAKTLTRKEIDKLTEKAKGIGAKGLAFIRWNDAKPTCSFAKFLPEGRLETILQTLGCEQGDVVLIVADKDKVTLPVLGALRLQVAKQLDLIPAGKFNFLWITQFPFFEWDDETETWVAMHHPFTMPMDECLPYLDTNPERVIAKAFDLVLNGTELSSGSIRITDYELQQKMFEALGLTDEEIEAKFGFLVEAYHYGAPPHGGLGIGLDRLTMVMLQAESLRDVVAFPKVQNASELMSECPAPVDTASLDVLGIQIKETSKTTDAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0R3</accession>
    <name>R5V0R3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Nicotinate phosphoribosyltransferase</fullName>
            <ecNumber evidence="4 8">6.3.4.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BN566_01913</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the first step in the biosynthesis of NAD from nicotinic acid, the ATP-dependent synthesis of beta-nicotinate D-ribonucleotide from nicotinate and 5-phospho-D-ribose 1-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O + nicotinate = ADP + diphosphate + nicotinate beta-D-ribonucleotide + phosphate</text>
            <dbReference type="Rhea" id="RHEA:36163"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32544"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from nicotinate: step 1/1.</text>
    </comment>
    <comment type="PTM">
        <text evidence="8">Transiently phosphorylated on a His residue during the reaction cycle. Phosphorylation strongly increases the affinity for substrates and increases the rate of nicotinate D-ribonucleotide production. Dephosphorylation regenerates the low-affinity form of the enzyme, leading to product release.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the NAPRTase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.21" evidence="4 8"/>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01913"/>
    <dbReference type="EnsemblBacteria" id="CCZ84188">
        <property type="protein sequence ID" value="CCZ84188"/>
        <property type="gene ID" value="BN566_01913"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00457"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004516">
        <property type="term" value="F:nicotinate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004514">
        <property type="term" value="F:nicotinate-nucleotide diphosphorylase (carboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041525">
        <property type="entry name" value="N/Namide_PRibTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041619">
        <property type="entry name" value="NAPRTase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040727">
        <property type="entry name" value="NAPRTase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007229">
        <property type="entry name" value="Nic_PRibTrfase-Fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006405">
        <property type="entry name" value="Nic_PRibTrfase_pncB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036068">
        <property type="entry name" value="Nicotinate_pribotase-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11098">
        <property type="entry name" value="PTHR11098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04095">
        <property type="entry name" value="NAPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17956">
        <property type="entry name" value="NAPRTase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17767">
        <property type="entry name" value="NAPRTase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000484">
        <property type="entry name" value="NAPRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51690">
        <property type="entry name" value="SSF51690"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01513">
        <property type="entry name" value="NAPRTase_put"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7 8" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NAPRTase_N" evidence="10">
        <location>
            <begin position="8"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="NAPRTase" evidence="9">
        <location>
            <begin position="154"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="NAPRTase_C" evidence="11">
        <location>
            <begin position="362"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CCZ84188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="479" mass="54022" checksum="642C41519BB8DE70" modified="2013-07-24" version="1">MKQENYAMLCDFYEFTMSNGYFRNGFYQKTVYFDVFFRDIPDGGGFAIAAGLEQVIDYVKELHFTEEDVAFLRSKQCFDEGFLTYLKEFHFSGDIWAVPEGTPVFPNEPIMTIRAPAIEAQLLETFILLSLNHQSLIATKANRIVRAADGRTVLEFGSRRAQGASGAILGARAAYIGGCAGTACTLTDELYGVPAGGTMAHSWVQMFDTEYEAFKAYCENYPHQATLLVDTYNTLKSGIPNAIRAFKEILLPQGITDFGIRLDSGDIAYLTKKARKMLDAAGLQSCKIVVSNSLDEYLIQDLMAQHAQIDVFGVGERMITAKSTPVFGGVYKLVAIEQPDGTIVPKIKISENITKITNPHFKKVYRFYDKETGKAIADELCLHNETIDASQPHTIFDPIATWKTKEITNYTVRELLVPIFKNGECVYDQPSLSEICQYCKEQIALLWDEVKRFENPHNYYVDLSHALWEVKQDLLKKNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0T1</accession>
    <name>R5V0T1_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 14">Undecaprenyl-diphosphatase</fullName>
            <ecNumber evidence="4 14">3.6.1.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13 14">Bacitracin resistance protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12 14">Undecaprenyl pyrophosphate phosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">uppP</name>
        <name evidence="15" type="ORF">BN566_01933</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the dephosphorylation of undecaprenyl diphosphate (UPP). Confers resistance to bacitracin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>di-trans,octa-cis-undecaprenyl diphosphate + H2O = di-trans,octa-cis-undecaprenyl phosphate + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:28094"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58405"/>
            <dbReference type="ChEBI" id="CHEBI:60392"/>
            <dbReference type="EC" id="3.6.1.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Cell membrane</location>
            <topology evidence="14">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="14">Bacitracin is thought to be involved in the inhibition of peptidoglycan synthesis by sequestering undecaprenyl diphosphate, thereby reducing the pool of lipid carrier available.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the UppP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.27" evidence="4 14"/>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01933"/>
    <dbReference type="EnsemblBacteria" id="CCZ84208">
        <property type="protein sequence ID" value="CCZ84208"/>
        <property type="gene ID" value="BN566_01933"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050380">
        <property type="term" value="F:undecaprenyl-diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016311">
        <property type="term" value="P:dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01006">
        <property type="entry name" value="Undec_diphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003824">
        <property type="entry name" value="UppP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30622">
        <property type="entry name" value="PTHR30622"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02673">
        <property type="entry name" value="BacA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00753">
        <property type="entry name" value="undec_PP_bacA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0046">Antibiotic resistance</keyword>
    <keyword evidence="6 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0133">Cell shape</keyword>
    <keyword evidence="14" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="121"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="204"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="234"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="267"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CCZ84208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="286" mass="31652" checksum="C56EBEFC32439402" modified="2013-07-24" version="1">MFWELLKALFLGIVEGVTEWLPISSTGHLILVDAFLKLDASDAFKEMFDVVIQLGAILAVVVIYWKNLWPFHKKETNPNPLTKTGFGACVQKNILQMWCKVLVACVPAAVVGLLFDDVFHALFYNPITVAIMLILFGVAFIVIERWQKTKQPRIHSIGGLDYKTVLLIGVFQLIAAVFPGTSRSGATIVGALLLGVSRTAAAEFTFYLAVPVMAGASLLKLVKFGFHFTGMEFAVLLVGMVSAFVVSIFIIRFLMDYIRKHDFTVFGWYRIVLGILVLIFAAVGVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0V8</accession>
    <name>R5V0V8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phospho-N-acetylmuramoyl-pentapeptide-transferase</fullName>
            <ecNumber evidence="8">2.7.8.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">UDP-MurNAc-pentapeptide phosphotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">mraY</name>
        <name evidence="9" type="ORF">BN566_01634</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the initial step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan: transfers peptidoglycan precursor phospho-MurNAc-pentapeptide from UDP-MurNAc-pentapeptide onto the lipid carrier undecaprenyl phosphate, yielding undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide, known as lipid I.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>di-trans,octa-cis-undecaprenyl phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine = di-trans-octa-cis-undecaprenyl diphospho-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine + UMP</text>
            <dbReference type="Rhea" id="RHEA:28386"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:60392"/>
            <dbReference type="ChEBI" id="CHEBI:61386"/>
            <dbReference type="ChEBI" id="CHEBI:61387"/>
            <dbReference type="EC" id="2.7.8.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="8">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the glycosyltransferase 4 family. MraY subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.13" evidence="8"/>
    <dbReference type="EMBL" id="CBAR010000172">
        <property type="protein sequence ID" value="CCZ83905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01634"/>
    <dbReference type="EnsemblBacteria" id="CCZ83905">
        <property type="protein sequence ID" value="CCZ83905"/>
        <property type="gene ID" value="BN566_01634"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008963">
        <property type="term" value="F:phospho-N-acetylmuramoyl-pentapeptide-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051992">
        <property type="term" value="F:UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine:undecaprenyl-phosphate transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06852">
        <property type="entry name" value="GT_MraY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00038">
        <property type="entry name" value="MraY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000715">
        <property type="entry name" value="Glycosyl_transferase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003524">
        <property type="entry name" value="PNAcMuramoyl-5peptid_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018480">
        <property type="entry name" value="PNAcMuramoyl-5peptid_Trfase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22926">
        <property type="entry name" value="PTHR22926"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00953">
        <property type="entry name" value="Glycos_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10555">
        <property type="entry name" value="MraY_sig1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00445">
        <property type="entry name" value="mraY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01347">
        <property type="entry name" value="MRAY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01348">
        <property type="entry name" value="MRAY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0133">Cell shape</keyword>
    <keyword evidence="7 8" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="135"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="164"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="195"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="222"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="275"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="325"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ83905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="347" mass="38478" checksum="468FDC99F5CE8DB0" modified="2013-07-24" version="1">MPLWSINTIVALLSFVIAAAFGKALIPFLHKLKFGQPIKMKFGPEWHAKKQGTPTMGGILFILSTTIAVGVGYGLYRGFCTNAEVSGVEQLDLNRGAQMFSCAIFAILFGLIGFIDDFKKVVQKKNDGLSPMQKIILQVVVAAAWLGVQYWLGDRDTRLDFSFFSFDISWFYYPLMLLVILYLTNAVNLTDGIDGLCGTVTMVNMLLMTVICVLLSLQQMSIFTMALAGGCLGFLVWNLHPAKCFMGDTGSMYLGASVVAVGLVTHQHLVLLLVALLYILEALSVVIQVTYFKYTKKKYGEGRRIFKMTPIHHHFELSGFSEYKIVITFSLFALVMGILGLLLVLFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>R5V101</accession>
    <name>R5V101_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Branched-chain-amino-acid aminotransferase</fullName>
            <ecNumber evidence="15">2.6.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">BN566_00207</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>2-oxoglutarate + L-isoleucine = (S)-3-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24801"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 15">
            <text>2-oxoglutarate + L-leucine = 4-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:18321"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:17865"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 15">
            <text>2-oxoglutarate + L-valine = 3-methyl-2-oxobutanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24813"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4 14">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5 16">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 4/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7 16">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 4/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6 16">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 4/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8 13">Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.42" evidence="15"/>
    <dbReference type="EMBL" id="CBAR010000207">
        <property type="protein sequence ID" value="CCZ84278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00207"/>
    <dbReference type="EnsemblBacteria" id="CCZ84278">
        <property type="protein sequence ID" value="CCZ84278"/>
        <property type="gene ID" value="BN566_00207"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00058"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00073"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00062"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052656">
        <property type="term" value="F:L-isoleucine transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052654">
        <property type="term" value="F:L-leucine transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050048">
        <property type="term" value="F:L-leucine:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052655">
        <property type="term" value="F:L-valine transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01557">
        <property type="entry name" value="BCAT_beta_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001544">
        <property type="entry name" value="Aminotrans_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018300">
        <property type="entry name" value="Aminotrans_IV_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036038">
        <property type="entry name" value="Aminotransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005786">
        <property type="entry name" value="B_amino_transII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043132">
        <property type="entry name" value="BCAT-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043131">
        <property type="entry name" value="BCAT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033939">
        <property type="entry name" value="BCAT_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11825">
        <property type="entry name" value="PTHR11825"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01063">
        <property type="entry name" value="Aminotran_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006468">
        <property type="entry name" value="BCAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56752">
        <property type="entry name" value="SSF56752"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01123">
        <property type="entry name" value="ilvE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00770">
        <property type="entry name" value="AA_TRANSFER_CLASS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9 15 17" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="15" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="11 14" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 15 17" id="KW-0808">Transferase</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="12">
        <location>
            <position position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00005072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00009320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR006468-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU004519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CCZ84278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="357" mass="40268" checksum="73EAD4B0FC8E8A95" modified="2013-07-24" version="1">MEIKKKLAATLKEKPQDESKLGFGHIFTDHMLIMPYDEGQGWHDPEIVPYDNISLSPAAMCFHYGQTVFEGLKAYRTADDKIQLFRPEENFKRLNRSNERLVIPELPVELGMQCLMELLEVEKDWVPHLEGTSLYIRPFIIAIDPFLGVRPGAQYLFMIILSPSGAYYASGLNPVNIYVEDKYVRTVRGCMGYAKTGGNYAASLHSQDDAHKQNYSQVLWLDGVEQKYIEEVGAMNIFFVIDGEIVTPSLETGSILAGITRKSTIELCKSWGLPVSERRITIQEIADAYDAGKLDEVFGTGTAAVISPVGHLKWDDKVMEINNNKIGKISQRIYDTLTGIQWGKLPDPFNWVVPLEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5V168</accession>
    <name>R5V168_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00246</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000215">
        <property type="protein sequence ID" value="CCZ84333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84333">
        <property type="protein sequence ID" value="CCZ84333"/>
        <property type="gene ID" value="BN566_00246"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="70" mass="8298" checksum="C2A276915D00D6E3" modified="2013-07-24" version="1">MNAMLESYRNSCNQLTIRISELSQQIQQTCSQTEADYLNSRKHLLQTERLELLRDMQDMQPYVRGNVHEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1A1</accession>
    <name>R5V1A1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Lactate dehydrogenase and related dehydrogenases</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_00270</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000220">
        <property type="protein sequence ID" value="CCZ84363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00270"/>
    <dbReference type="EnsemblBacteria" id="CCZ84363">
        <property type="protein sequence ID" value="CCZ84363"/>
        <property type="gene ID" value="BN566_00270"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00670">
        <property type="entry name" value="D_2_HYDROXYACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2-Hacid_dh" evidence="4">
        <location>
            <begin position="22"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="2-Hacid_dh_C" evidence="5">
        <location>
            <begin position="109"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ84363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="317" mass="33940" checksum="F14C366B4997FAD6" modified="2013-07-24" version="1">MKKLVITDWSTLSMTDDLSTAALQELADVTAYPLTAPEEVAARIGDAELLLCNKTPITAAVLDACPNLEYIGVMATGYNNIDLKAAVQHGITVCNAGSYSTMAVAQHTFALMLHHFSRIAAYDRAVRAGAWEKSLQFSWFPYPTAELAGKTLCVVGYGSIGKAVAKIGQVFGMQVKITTRTTPSNCPFPVISMEEAFQTADVLTIHTPLTPETQCLVNAERLASMKPTAFLINTARGGIVDETALAEALRSGVIAGAAVDVLDTEPMSDQTPLRQLPNCTITPHVAWAAVETRNRLLQIVTENVAAYLNGTPQNVVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1G4</accession>
    <name>R5V1G4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">The GLUG motif protein family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00320</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000225">
        <property type="protein sequence ID" value="CCZ84413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00320"/>
    <dbReference type="EnsemblBacteria" id="CCZ84413">
        <property type="protein sequence ID" value="CCZ84413"/>
        <property type="gene ID" value="BN566_00320"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003343">
        <property type="entry name" value="Big_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008964">
        <property type="entry name" value="Invasin/intimin_cell_adhesion"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02368">
        <property type="entry name" value="Big_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49373">
        <property type="entry name" value="SSF49373"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004394921" evidence="2">
        <location>
            <begin position="36"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="3">
        <location>
            <begin position="554"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="376"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="671" mass="70733" checksum="403C09FD59F30491" modified="2013-07-24" version="1">MEKLRKNTCRKAISFFVTGLLTIACLPANVSVFSAAWNGTVDTSWYQAGETQLSISNPEELAGLAKLVNDGTTNFEGVTITLEADLQMNPLTNWEKWETAEAQPSNTWVPIGIVNQSLFHGTFDGNGHTISGLYAQKSGETGLFAGLYRTATVKNLTIEKSYLSSCDTFAGGICGYNYKGTIENCENRAVISSWYYMAGGICGVNFNGTVSRCSNDAFISGQEAAGGIAGQSIYAIVSESCSTGRVSSLQFAGGVVGQESGGIVQSCYNTGAIKGNTIAGGVVGAFECGSIETCFNIGTVSGNEKIGGIVGESNANCTACYYQSGVTDTQPIDREICTAMKQSEMQKATFAQKLGAAYSGITGSYPLLSWQQRHAQTTETTTDATTAGTTKATATTTTTKKATTGTTKPSNTTTSARTTQTTTITSLEQPVTGTTLEGDGRVVTQKNIKEIHQIGATLQLYYLGEKGEVPQWVSTDDSVATIDQDGVLTAVSEGSVIVGALVNQKFYKLTITISVPKTTAETTIPIETETTDLTTATTEPVTTTIVTTTATLPDPYEMGDVDGDSTVSVDDAIQILTYYARRAAGYTTFFSEIAHINDLATSAADINGDGVISVEDAVAVLTYYAKTASGMQPSWADVIGTSSKQANTESGEPVESLLPEEFALLPMEGTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1G5</accession>
    <name>R5V1G5_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glycogen synthase</fullName>
            <ecNumber evidence="7">2.4.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Starch [bacterial glycogen] synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">glgA</name>
        <name evidence="10" type="ORF">BN566_01821</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 7">Synthesizes alpha-1,4-glucan chains using ADP-glucose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>[(1-&gt;4)-alpha-D-glucosyl](n) + ADP-alpha-D-glucose = [(1-&gt;4)-alpha-D-glucosyl](n+1) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18189"/>
            <dbReference type="Rhea" id="RHEA-COMP:9584"/>
            <dbReference type="Rhea" id="RHEA-COMP:9587"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15444"/>
            <dbReference type="ChEBI" id="CHEBI:57498"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.4.1.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Glycan biosynthesis; glycogen biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the glycosyltransferase 1 family. Bacterial/plant glycogen synthase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.21" evidence="7"/>
    <dbReference type="EMBL" id="CBAR010000186">
        <property type="protein sequence ID" value="CCZ84095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01821"/>
    <dbReference type="EnsemblBacteria" id="CCZ84095">
        <property type="protein sequence ID" value="CCZ84095"/>
        <property type="gene ID" value="BN566_01821"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00164"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033201">
        <property type="term" value="F:alpha-1,4-glucan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004373">
        <property type="term" value="F:glycogen (starch) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009011">
        <property type="term" value="F:starch synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00484">
        <property type="entry name" value="Glycogen_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011835">
        <property type="entry name" value="GS/SS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013534">
        <property type="entry name" value="Starch_synth_cat_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08323">
        <property type="entry name" value="Glyco_transf_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02095">
        <property type="entry name" value="glgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0320">Glycogen biosynthesis</keyword>
    <keyword evidence="4 7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_transf_5" evidence="9">
        <location>
            <begin position="3"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="8">
        <location>
            <begin position="295"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>ADP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:57498"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ84095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="487" mass="56517" checksum="D9EE224AAE2D3466" modified="2013-07-24" version="1">MKNILFAASECVPFVKTGGLADVVGALPKALYKGDYDVRVILPNYTCIPWEYRGKFKFVSNFYMDYGQRSEPMFVGIMEYEYEGITFYFIDNEYYFKCDKPYTDSVRWDIERYIFFCKAVLAAMPVIGFHPDIIHCHDWQTGMIPVFLKSTFATHSFYWGTKSIMTIHNLKFQGVWDIDHFIYNTNLPGYMFTPDKLEFKKDANMLKGGLVYADYITTVSETYAEEIKTPYYGEQLDGLLCARSRSLRGILNGIDYSVYNPETDPKIAVHYNEKNAVEKKKEAKRALQKELGLPVDDSKFMIAIISRLTDQKGLDLVDWITERIVDEFTQFVIIGTGESRYEDMFRYYQGRFPDRVSANIFYSDALAHKLYAAADAMLVPSRFEPCGLTQLISLRYGTVPIVRETGGLKDTVEPYNEFEKCGTGFTFHNYNADDLLYTINYAKNIYFNHREDWNDIIQRGMEKDFSWGSSALQYQGMYDWILRGMQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1J5</accession>
    <name>R5V1J5_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phospho-2-dehydro-3-deoxyheptonate aldolase</fullName>
            <ecNumber evidence="5">2.5.1.54</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_01860</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 5">Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>D-erythrose 4-phosphate + H2O + phosphoenolpyruvate = 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14717"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16897"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="2.5.1.54"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 5">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 1/7.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the class-I DAHP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.54" evidence="5"/>
    <dbReference type="EMBL" id="CBAR010000192">
        <property type="protein sequence ID" value="CCZ84135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01860"/>
    <dbReference type="EnsemblBacteria" id="CCZ84135">
        <property type="protein sequence ID" value="CCZ84135"/>
        <property type="gene ID" value="BN566_01860"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00084"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003849">
        <property type="term" value="F:3-deoxy-7-phosphoheptulonate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006218">
        <property type="entry name" value="DAHP1/KDSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006219">
        <property type="entry name" value="DHAP_synth_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21225">
        <property type="entry name" value="PTHR21225"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00793">
        <property type="entry name" value="DAHP_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001361">
        <property type="entry name" value="DAHP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00034">
        <property type="entry name" value="aroFGH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAHP_synth_1" evidence="6">
        <location>
            <begin position="37"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR001361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ84135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="342" mass="38526" checksum="68565C5FCE501667" modified="2013-07-24" version="1">MNMNFIRKLPIPQELKKQFSLSDELVAVKAARDAEIQKVFTGESDKKLLIIGPCSADREDAVLDYVCRLAKVQEQVKDKLILIPRIYTNKPRTTGEGYKGMVHQPDPEKKEDMLQGVIAIRQMHTKAVEMTGLTCADEMLYPENHRYLSDLLSYVAVGARSVEDQQHRLTASGLDIPVGMKNPTSGDLSVMMNSLIAAHASHTFLYRGWEVQTAGNPLSHAILRGYVDSQGHSMPNYHYEDLMHLCELYDQKKLADPAVIVDANHANSGKKYLEQIRICNEVLHSCRHSDDIRKLFKGFMIESYIEDGAQKIGEGVYGKSITDPCLGWEKSEKLIYTIADQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1M6</accession>
    <name>R5V1M6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01900</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 4-hydroxybenzoyl-CoA thioesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000193">
        <property type="protein sequence ID" value="CCZ84175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01900"/>
    <dbReference type="EnsemblBacteria" id="CCZ84175">
        <property type="protein sequence ID" value="CCZ84175"/>
        <property type="gene ID" value="BN566_01900"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006684">
        <property type="entry name" value="YbgC/YbaW"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003230">
        <property type="entry name" value="YbgC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00051">
        <property type="entry name" value="TIGR00051"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="145" mass="17039" checksum="175709EA214B1D3A" modified="2013-07-24" version="1">MHHIITPESIVYQRPVQYYETDRMQIVHHSNYIRWMEEIRVAFMAQKGVPYDQMEHNGILMPVLGVTCSYHIPFQFGETFQATCKITEYNGVRLALSYEIYKPGESRCCVTGTSQHCFTNNDRKPLRLPRQNPELHQLFLSLLES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1X3</accession>
    <name>R5V1X3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">50S ribosomal protein L5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rplE</name>
        <name evidence="9" type="ORF">BN566_00069</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">This is 1 of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. In the 70S ribosome it contacts protein S13 of the 30S subunit (bridge B1b), connecting the 2 subunits; this bridge is implicated in subunit movement. Contacts the P site tRNA; the 5S rRNA and some of its associated proteins might help stabilize positioning of ribosome-bound tRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the 50S ribosomal subunit; part of the 5S rRNA/L5/L18/L25 subcomplex. Contacts the 5S rRNA and the P site tRNA. Forms a bridge to the 30S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the universal ribosomal protein uL5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000241">
        <property type="protein sequence ID" value="CCZ84523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00069"/>
    <dbReference type="EnsemblBacteria" id="CCZ84523">
        <property type="protein sequence ID" value="CCZ84523"/>
        <property type="gene ID" value="BN566_00069"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01333_B">
        <property type="entry name" value="Ribosomal_L5_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002132">
        <property type="entry name" value="Ribosomal_L5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020930">
        <property type="entry name" value="Ribosomal_L5_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031309">
        <property type="entry name" value="Ribosomal_L5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020929">
        <property type="entry name" value="Ribosomal_L5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_L5_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031310">
        <property type="entry name" value="Ribosomal_L5_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11994">
        <property type="entry name" value="PTHR11994"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00281">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00673">
        <property type="entry name" value="Ribosomal_L5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002161">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55282">
        <property type="entry name" value="SSF55282"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00358">
        <property type="entry name" value="RIBOSOMAL_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="5" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Ribosomal_L5" evidence="7">
        <location>
            <begin position="24"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal_L5_C" evidence="8">
        <location>
            <begin position="84"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ84523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="179" mass="20103" checksum="EF0EB42492304527" modified="2013-07-24" version="1">MSTLKDYYNSTVAPAMMKKFGYQNVMQIPKLDKVVVNVAAGEAKDNAKVIDAIMNDLSLITGQKPVVCRAKKSVANFKLREGMPIGVKVTLRGEKMYEFVYKLFNVSFPRVRDFRGINGNSFDGKGNYSTGIKEQLIFPEIEYDKIDKVRGMDINFITTAQTDEEAKELLTLLGAPFIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1Y4</accession>
    <name>R5V1Y4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">30S ribosomal protein S11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rpsK</name>
        <name evidence="9" type="ORF">BN566_00084</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Located on the platform of the 30S subunit, it bridges several disparate RNA helices of the 16S rRNA. Forms part of the Shine-Dalgarno cleft in the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Part of the 30S ribosomal subunit. Interacts with proteins S7 and S18. Binds to IF-3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the universal ribosomal protein uS11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000241">
        <property type="protein sequence ID" value="CCZ84538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00084"/>
    <dbReference type="EnsemblBacteria" id="CCZ84538">
        <property type="protein sequence ID" value="CCZ84538"/>
        <property type="gene ID" value="BN566_00084"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01310">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001971">
        <property type="entry name" value="Ribosomal_S11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019981">
        <property type="entry name" value="Ribosomal_S11_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018102">
        <property type="entry name" value="Ribosomal_S11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036967">
        <property type="entry name" value="Ribosomal_S11_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11759">
        <property type="entry name" value="PTHR11759"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00411">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002131">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03632">
        <property type="entry name" value="uS11_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00054">
        <property type="entry name" value="RIBOSOMAL_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 7 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 7" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ84538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="133" mass="14389" checksum="B478FADA443F84A3" modified="2013-07-24" version="1">MAQQKGKKVVIRRRRERKNIENGAVHIQSTFNNTIVTITDTQGNAISWASSGGLGFRGSRKSTPFAAQTAAETAAKAAMEHGLKSVEVYVKGPGAGREAAIRALQTVGLEVKMIKDVTPIPHNGCRPPKRRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>R5V228</accession>
    <name>R5V228_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Bifunctional ligase/repressor BirA</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Biotin--[acetyl-CoA-carboxylase] ligase</fullName>
            <ecNumber evidence="2">6.3.4.15</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Biotin--protein ligase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Biotin-[acetyl-CoA carboxylase] synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">birA</name>
        <name evidence="4" type="ORF">BN566_00449</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Acts both as a biotin--[acetyl-CoA-carboxylase] ligase and a repressor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + biotin + L-lysyl-[protein] = AMP + diphosphate + H(+) + N(6)-biotinyl-L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:11756"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10505"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
            <dbReference type="ChEBI" id="CHEBI:83144"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.15"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the biotin--protein ligase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.15" evidence="2"/>
    <dbReference type="EMBL" id="CBAR010000248">
        <property type="protein sequence ID" value="CCZ84588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00449"/>
    <dbReference type="EnsemblBacteria" id="CCZ84588">
        <property type="protein sequence ID" value="CCZ84588"/>
        <property type="gene ID" value="BN566_00449"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004077">
        <property type="term" value="F:biotin-[acetyl-CoA-carboxylase] ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16442">
        <property type="entry name" value="BPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00978">
        <property type="entry name" value="Bifunct_BirA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030855">
        <property type="entry name" value="Bifunct_BirA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004408">
        <property type="entry name" value="Biotin_CoA_COase_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003142">
        <property type="entry name" value="BPL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013196">
        <property type="entry name" value="HTH_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008988">
        <property type="entry name" value="Transcriptional_repressor_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02237">
        <property type="entry name" value="BPL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03099">
        <property type="entry name" value="BPL_LplA_LipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08279">
        <property type="entry name" value="HTH_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50037">
        <property type="entry name" value="SSF50037"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00121">
        <property type="entry name" value="birA_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 2" id="KW-0092">Biotin</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="3">
        <location>
            <begin position="70"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="2">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Biotin binding" evidence="2">
        <location>
            <begin position="93"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Biotin binding" evidence="2">
        <location>
            <begin position="121"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>biotin</name>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>biotin</name>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="330" mass="36113" checksum="9353D4DA70319AAD" modified="2013-07-24" version="1">MKRKDALYELLIQHAGTYCSGETLAQELGISRAAVWKAVQTLRTESNVPIQSHPKKGYCIPADADLLTVAAVQPHLKTQYLGRVLYTHTTINSTNTACKELAAAYAVHGTVVAADGQSAGRGRMGRPFCSPVNTGLYFSILLRKGLMMPMAQQVTCCAAVAVAQAIEALYQLPVQIKWVNDIFYQGKKLCGILTEAAWNCEDGRMDYMVVGIGINIRQTAVTLPPELHAIVSSLEESGCAHVRRAELLAEVLNRFEVLLETLPEHGFLEEYRRRSCLTGQRVTAWQGTQQREGVVTGIAEDGALLMQLDSGETIALQSGEVTLHQPEETE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>R5V237</accession>
    <name>R5V237_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Stage 0 sporulation protein A homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN566_00092</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000252">
        <property type="protein sequence ID" value="CCZ84598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00092"/>
    <dbReference type="EnsemblBacteria" id="CCZ84598">
        <property type="protein sequence ID" value="CCZ84598"/>
        <property type="gene ID" value="BN566_00092"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Response regulatory" evidence="9">
        <location>
            <begin position="3"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="10">
        <location>
            <begin position="132"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="8">
        <location>
            <begin position="132"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="7">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ84598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="235" mass="27171" checksum="21D73D6E6DD5A33E" modified="2013-07-24" version="1">MKRILVCEDEDVIREFVVINLKRVGYEVYDVNCGEEALRVFDAANGNFDIVLLDIMMPGIDGFQVCKELRKRSSTIGIIMLSAKTQEMDKVNGLMIGADDYITKPFSPSELTARVDAIYRRVCMSSMSDKQKSVLKSGPFVLNLVNRTLHKQEKPIELTQIEYQVLEYFLKNKNTALDRSSILQHIWGEQYDGDDKIVDVNIRRIRMKIEDQPSNPKYITTIWGYGYKWNDPDDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>R5V2A8</accession>
    <name>R5V2A8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribosomal protein L11 methyltransferase</fullName>
            <shortName evidence="2">L11 Mtase</shortName>
            <ecNumber evidence="2">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">prmA</name>
        <name evidence="3" type="ORF">BN566_00049</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Methylates ribosomal protein L11.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>L-lysyl-[protein] + 3 S-adenosyl-L-methionine = 3 H(+) + N(6),N(6),N(6)-trimethyl-L-lysyl-[protein] + 3 S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:54192"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:13826"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the methyltransferase superfamily. PrmA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CBAR010000230">
        <property type="protein sequence ID" value="CCZ84450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00049"/>
    <dbReference type="EnsemblBacteria" id="CCZ84450">
        <property type="protein sequence ID" value="CCZ84450"/>
        <property type="gene ID" value="BN566_00049"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00735">
        <property type="entry name" value="Methyltr_PrmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004498">
        <property type="entry name" value="Ribosomal_PrmA_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000401">
        <property type="entry name" value="RPL11_MTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00406">
        <property type="entry name" value="prmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="322" mass="35799" checksum="B7DDBCEE56E15DBB" modified="2013-07-24" version="1">MNWTQVDIYTTTDGIDLLCSDLMDLGIRGFSIQDAEDFNEFLQNKDGKWDYIDEDLMGLSHCESCVTIYLPEDAQGAETLQMVQELLKRKKAADTEGKFGRLEAVLTGVKEEDWANNWKQYFKPFPVGEKLYIRPSWEHDEPEAGRTVLEIDPESSFGTGQHHTTRLCLELMESEAAGSKMLDMGCGSGILFIGGMLLGAKEVFAVDIEENAVRIAKKNAAENHLPEEKYTVRCGNVLSDEALRAEIGSGYDLITANIVADVLKAMAPLFPQFLKKTGTLIISGIIVERKDEVIAAMEAQGFVVTDCREKEGWAAVKLKQAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5V2D1</accession>
    <name>R5V2D1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein conserved in bacteria</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00364</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000238">
        <property type="protein sequence ID" value="CCZ84465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00364"/>
    <dbReference type="EnsemblBacteria" id="CCZ84465">
        <property type="protein sequence ID" value="CCZ84465"/>
        <property type="gene ID" value="BN566_00364"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003790">
        <property type="entry name" value="GHL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02638">
        <property type="entry name" value="GHL10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004406652" evidence="2">
        <location>
            <begin position="23"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="GHL10" evidence="3">
        <location>
            <begin position="68"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="427" mass="47479" checksum="697A2A90854F8C51" modified="2013-07-24" version="1">MKIQVVGSFFLIACLLTGCADSEKLESAPPNALFCKNITENVQTIQHLHLPATPENPISTASTAFPCGMWFTAMDYAEILTGKSEAEFRAAIQERFQQVQALGIQTVFLQVRAFADAYYTSTYYMSGLSMSADCSFDPLQIMLTEAHQLGLSVHAWVNPLRCQTDAQMEQMPEQYPISQWYADETKRGTWLVKSGDRWWLNPAYPEVRQLIADGVSELVTQYEIDGVQIDDYFYPTTDASFDAAAFAESGATDRSAWRKEQCNLMVQAMYNAVKQANPKVLFGISPQGTLSGNEKQSADVQTWGQTAGYCDYLLPQLYFGFQNSTAPFAETAAFWAEQVTCPDVSLWIGICTYKIGQQDTWAGDGMTEWQTDSQVVSKELELLCNMDGIDGAAIYDYASTFQPESSVSEILSSERDAIQVLLQTPAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>R5V2P3</accession>
    <name>R5V2P3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">DNA-directed RNA polymerase subunit alpha</fullName>
            <shortName evidence="11">RNAP subunit alpha</shortName>
            <ecNumber evidence="2 11">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">RNA polymerase subunit alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9 11">Transcriptase subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">rpoA</name>
        <name evidence="13" type="ORF">BN566_00086</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 11">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer. The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.</text>
    </comment>
    <comment type="domain">
        <text evidence="11">The N-terminal domain is essential for RNAP assembly and basal transcription, whereas the C-terminal domain is involved in interaction with transcriptional regulators and with upstream promoter elements.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the RNA polymerase alpha chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="2 11"/>
    <dbReference type="EMBL" id="CBAR010000241">
        <property type="protein sequence ID" value="CCZ84540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00086"/>
    <dbReference type="EnsemblBacteria" id="CCZ84540">
        <property type="protein sequence ID" value="CCZ84540"/>
        <property type="gene ID" value="BN566_00086"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.120.12">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00059">
        <property type="entry name" value="RNApol_bact_RpoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011262">
        <property type="entry name" value="DNA-dir_RNA_pol_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011263">
        <property type="entry name" value="DNA-dir_RNA_pol_RpoA/D/Rpb3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011773">
        <property type="entry name" value="DNA-dir_RpoA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011260">
        <property type="entry name" value="RNAP_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036643">
        <property type="entry name" value="RNApol_insert_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32108">
        <property type="entry name" value="PTHR32108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01000">
        <property type="entry name" value="RNA_pol_A_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03118">
        <property type="entry name" value="RNA_pol_A_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01193">
        <property type="entry name" value="RNA_pol_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00662">
        <property type="entry name" value="RPOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="SSF55257"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56553">
        <property type="entry name" value="SSF56553"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02027">
        <property type="entry name" value="rpoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11 13" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="6 11" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RPOLD" evidence="12">
        <location>
            <begin position="21"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Alpha N-terminal domain (alpha-NTD)" evidence="11">
        <location>
            <begin position="1"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Alpha C-terminal domain (alpha-CTD)" evidence="11">
        <location>
            <begin position="249"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CCZ84540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="317" mass="35167" checksum="F1A2D24B3022BDF5" modified="2013-07-24" version="1">MLEKIEKPELETVELRSDGKYGKFVLAPLERGYGTTLGNSLRRVLLSSLPGVAVNSVKIDGVHHELSTIPGVKEDVTEIILSLKGLTAKLYGDGPKTIYIEAEGECEVTAGDIKTDSEVNILDPGMHIATLGKDAKLYMEIVIDRGRGYVSAERNKQMINSPIDVIAVDSIYTPVLKVNYQVEDTRLGQVTDYDKLTLEVWTDGTISAKEAISQAANLLNEHLKLFVDLSDESSITEVLVEKNEKGKEKILEMTIEELDLSVRSFNCLKRAGINTVDDLINKSEEEMMKVRNLGKKSFDEVREKLQSLGFDLSSDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>R5V343</accession>
    <name>R5V343_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Hemolysins and related proteins containing CBS domains</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN566_00553</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0053 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000272">
        <property type="protein sequence ID" value="CCZ84700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00553"/>
    <dbReference type="EnsemblBacteria" id="CCZ84700">
        <property type="protein sequence ID" value="CCZ84700"/>
        <property type="gene ID" value="BN566_00553"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04590">
        <property type="entry name" value="CBS_pair_CorC_HlyC_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002550">
        <property type="entry name" value="CNNM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044751">
        <property type="entry name" value="Ion_transp-like_CBS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005170">
        <property type="entry name" value="Transptr-assoc_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03471">
        <property type="entry name" value="CorC_HlyC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01595">
        <property type="entry name" value="DUF21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01091">
        <property type="entry name" value="CorC_HlyC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="SSF56176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0129">CBS domain</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="100"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="10">
        <location>
            <begin position="214"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="10">
        <location>
            <begin position="280"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ84700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="441" mass="48718" checksum="C868E846E93C3BAF" modified="2013-07-24" version="1">MDTVRLWIFGFFAAAILLLLLHLWIASGIAALKELRPKWKTIALPQFSPTLQNLLKKPKLLKRTGTAQQILFHALFPCLLAGGLDPIFRLSLPDWITNAGFAAILLAATAVGSLLIFILSAALPLRVCKDPERRLTSHQKAFSFLLCLLKPLESVIAALISAVCGKDAVAETVTEEEILSIVDAGTENGLIATQQKEMIDNILEFDDVDVSDVMTHRRNIVGVEINTKIKDVVYLAVNESYSRMPVYRENIDNIIGVLIVKDLLALVGAEHVEHFNIQHFMREVLYVPETAKCKNVLTDMTRHNAQMAIAVDEYGGTAGIVTVEDILEEIVGSIRDEYDEEEAEQELRMVSDHVYIIQGSADPEDVLPKLGVGLPEGKTFDTMSGFLVDLLGRIPDAEETPVVTYDSVRFTVLLIEENWIAKIKAEVGVSEESTANVQAES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>R5V3Y8</accession>
    <name>R5V3Y8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glucose-6-phosphate isomerase</fullName>
            <shortName evidence="7">GPI</shortName>
            <ecNumber evidence="7">5.3.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Phosphoglucose isomerase</fullName>
            <shortName evidence="7">PGI</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Phosphohexose isomerase</fullName>
            <shortName evidence="7">PHI</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pgi</name>
        <name evidence="9" type="ORF">BN566_00634</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the reversible isomerization of glucose-6-phosphate to fructose-6-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7 8">
            <text>alpha-D-glucose 6-phosphate = beta-D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:11816"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="EC" id="5.3.1.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 7 8">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7 8">Belongs to the GPI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.9" evidence="7"/>
    <dbReference type="EMBL" id="CBAR010000012">
        <property type="protein sequence ID" value="CCZ82829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00634"/>
    <dbReference type="EnsemblBacteria" id="CCZ82829">
        <property type="protein sequence ID" value="CCZ82829"/>
        <property type="gene ID" value="BN566_00634"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00181"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004347">
        <property type="term" value="F:glucose-6-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05015">
        <property type="entry name" value="SIS_PGI_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05016">
        <property type="entry name" value="SIS_PGI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00473">
        <property type="entry name" value="G6P_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001672">
        <property type="entry name" value="G6P_Isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018189">
        <property type="entry name" value="Phosphoglucose_isomerase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035476">
        <property type="entry name" value="SIS_PGI_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035482">
        <property type="entry name" value="SIS_PGI_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11469">
        <property type="entry name" value="PTHR11469"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00342">
        <property type="entry name" value="PGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00662">
        <property type="entry name" value="G6PISOMERASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SSF53697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00765">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00174">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51463">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 7 8" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="4 7 8" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="5 7 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="285"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ82829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="444" mass="48985" checksum="70A0CE07BCD3C3AC" modified="2013-07-24" version="1">MALKLNTKYLASFVGEDELTGIAPAVNACAKVLHDKTGLGNDFLGWLTLPTDYDKEEFARIQAAAKKIQSNSDILIVIGIGGSYLGARAAIEFLQSTFYNNKKKDTPDIYYVGNNINPSYLQEVLAICEGKDISVNVISKSGTTTEPALAFRVFKKLVEEKYGKEGAKDRIFATTDKAKGTLKNLSDTEGYETFVVPDDVGGRYSVLTAVGLLPIAVAGCDIQALMDGAAQAQKDYMADFENNDCYKYAALRNILYRKGKSVEMLVSYDPNFVMMSEWYKQLFGESEGKDNKGIYPAAGVFSTDLHSMGQYIQEGSRILFETVVDIKKPKQDFFLEPDAENLDGLNFLTNQNMSVVNRKALEGVILAHTEGGVPNMILELDDTSEKSLGYLIYFFEKACAISGYLLGVNPFNQPGVESYKKNMFALLGKPGYEDRKAELEAKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5V402</accession>
    <name>R5V402_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">S4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00649</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000012">
        <property type="protein sequence ID" value="CCZ82844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00649"/>
    <dbReference type="EnsemblBacteria" id="CCZ82844">
        <property type="protein sequence ID" value="CCZ82844"/>
        <property type="gene ID" value="BN566_00649"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025490">
        <property type="entry name" value="RNAbp_HP1423"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038881">
        <property type="entry name" value="RNAbp_HP1423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S4" evidence="1">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="80" mass="8991" checksum="E9DA06B9A2434E40" modified="2013-07-24" version="1">MRLDKYLKVSRLIKRRTVANEACDAEKVLVNGKVARASYDVKVGDVIEINMGQKALKVKVEQVLEHVLKDDAAGMYMVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5V4S5</accession>
    <name>R5V4S5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00891</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000059">
        <property type="protein sequence ID" value="CCZ83104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83104">
        <property type="protein sequence ID" value="CCZ83104"/>
        <property type="gene ID" value="BN566_00891"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="84" mass="9683" checksum="DC76A62C5128FF2B" modified="2013-07-24" version="1">MSECQIITISDDKLSCEATAILLRMLNFPDTDYHTAEELCPFFENDSLKTIRNALNELYNAGYLRRSGKTYMVNKVRITQMKLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5V5S6</accession>
    <name>R5V5S6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">LPXTG-site transpeptidase (Sortase) family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00130</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000113">
        <property type="protein sequence ID" value="CCZ83474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00130"/>
    <dbReference type="EnsemblBacteria" id="CCZ83474">
        <property type="protein sequence ID" value="CCZ83474"/>
        <property type="gene ID" value="BN566_00130"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05828">
        <property type="entry name" value="Sortase_D_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005754">
        <property type="entry name" value="Sortase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041999">
        <property type="entry name" value="Sortase_D_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023365">
        <property type="entry name" value="Sortase_dom-sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04203">
        <property type="entry name" value="Sortase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63817">
        <property type="entry name" value="SSF63817"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01076">
        <property type="entry name" value="sortase_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="234" mass="25580" checksum="40B8ECD290C5C5E7" modified="2013-07-24" version="1">MKQKSSKPEKTSASALPLAMPLILPVVTLLLCAAIVLVCSLRPYEKLHTYLQIAFMDDLKQAPSSSSDGLEIVQNDITTNYSGKIAKTGEPVYPAFGEQYGVLSCEAIDLYVPVYWGATSELLELGACQTTASAIAGAGGNAVISAHVNTFFNRLDELQTGDTLTLYTNYGRFTYAVQKSIQFENTDKQYILPTDQEQLTLYTCEMQVFGSSTKRIGVVCDCISTEFYETEAAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5V5U4</accession>
    <name>R5V5U4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01258</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000117">
        <property type="protein sequence ID" value="CCZ83489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83489">
        <property type="protein sequence ID" value="CCZ83489"/>
        <property type="gene ID" value="BN566_01258"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="55" mass="6294" checksum="1DD4599ADE59D2CC" modified="2013-07-24" version="1">MDSPLHITGIFGKNLYANVQVSKRIYSFVKKIGGCDKNKSKNLYEMPNGWEQLCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5V626</accession>
    <name>R5V626_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01322</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000130">
        <property type="protein sequence ID" value="CCZ83554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83554">
        <property type="protein sequence ID" value="CCZ83554"/>
        <property type="gene ID" value="BN566_01322"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="58" mass="6672" checksum="5FDFFDD10955CD9E" modified="2013-07-24" version="1">MEYEKQPQTTTDAPVKIYQPQVKSVTVRQQTGFLDHAPVRIYEKPPKQQASPFPPAFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5V692</accession>
    <name>R5V692_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01375</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000147">
        <property type="protein sequence ID" value="CCZ83639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83639">
        <property type="protein sequence ID" value="CCZ83639"/>
        <property type="gene ID" value="BN566_01375"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="80" mass="9508" checksum="CEC7552CEB25A86C" modified="2013-07-24" version="1">MLVYLKLLFQNENIVVYEYGLTEEKMIGKVIVSLHNKKDCQFFSYSENYKSFNVYTGCVITKIFHYIAENRFPKTDLYAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>R5V6F3</accession>
    <name>R5V6F3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Amidophosphoribosyltransferase</fullName>
            <shortName evidence="5">ATase</shortName>
            <ecNumber evidence="5">2.4.2.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Glutamine phosphoribosylpyrophosphate amidotransferase</fullName>
            <shortName evidence="5">GPATase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">purF</name>
        <name evidence="11" type="ORF">BN566_01445</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = 5-phospho-alpha-D-ribose 1-diphosphate + H2O + L-glutamine</text>
            <dbReference type="Rhea" id="RHEA:14905"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="EC" id="2.4.2.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="5 8">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5 9">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="5 9">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 5 6">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5 6">In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.14" evidence="5"/>
    <dbReference type="EMBL" id="CBAR010000154">
        <property type="protein sequence ID" value="CCZ83709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01445"/>
    <dbReference type="EnsemblBacteria" id="CCZ83709">
        <property type="protein sequence ID" value="CCZ83709"/>
        <property type="gene ID" value="BN566_01445"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00124"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004044">
        <property type="term" value="F:amidophosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00715">
        <property type="entry name" value="GPATase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01931">
        <property type="entry name" value="PurF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005854">
        <property type="entry name" value="PurF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035584">
        <property type="entry name" value="PurF_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000485">
        <property type="entry name" value="Amd_phspho_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01134">
        <property type="entry name" value="purF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="5 6 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5 9" id="KW-0408">Iron</keyword>
    <keyword evidence="5 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 5 6" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="10">
        <location>
            <begin position="8"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5 7">
        <location>
            <position position="8"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5 9">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 8">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 8">
        <location>
            <position position="357"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 8">
        <location>
            <position position="358"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 9">
        <location>
            <position position="394"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 9">
        <location>
            <position position="448"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 9">
        <location>
            <position position="451"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ83709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="475" mass="52421" checksum="1B48BFA4BB7BE0A9" modified="2013-07-24" version="1">MTGLHEECGVFGVYAPHTTDVAAMTYAALYALQHRGQESCGIVVNDRGVFAHHKDLGLVPEVFKAEDMEKLGHGNISIGHVRYSTTGKPNRANAQPIVVRHVKGSMSIAHNGNLTNSRELRERYELKGAIFHTTNDTEVISYAITEQRLQRSSIEEAVEYAMYDLKGAYSLVVMSPNKLIAARDPHGFRPLCMGRCKDGSIVFASESCALDSVDAEFERDIEPGEIVVVEDCNVHSIRTHCGQKRSMCVFEYVYFARPDSVIEGTCVHTARLNAGRILWQEHPVEADVVVGVPDSGLDAALGLSMASGIPYGIGFVKNRYVGRTFIQPSQKMRTNSVRIKLNVLKSVVKGKRVILVDDSIVRGTTCKRIVNLLREAGATEVHMRISCPPFTNPCYFGVDIDSRENLIACQMTIPEICSYIGADSLGYLSLKGVKSLTSPDLQEEDWFCTGCFDGTYPIAVPKEMPKDKFESKIGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5V6N2</accession>
    <name>R5V6N2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Lysophospholipase L1 and related esterases</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01514</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000161">
        <property type="protein sequence ID" value="CCZ83779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83779">
        <property type="protein sequence ID" value="CCZ83779"/>
        <property type="gene ID" value="BN566_01514"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004406791" evidence="1">
        <location>
            <begin position="30"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="478" mass="54381" checksum="6B071B06F96A4DBC" modified="2013-07-24" version="1">MKFLKKGLAVLFACTFVSVPLYHPVKADAVSMDDMGDMNEDQEVSVSDAVEILTIYAKSAAGQLPSVTERKRFVADVNYDVQITVEDAVYVLTYYAKTAAGLSPSWESLGISMPQRCPETASYKRIHTTETETGNLVNVSFLDIDDDGKKETVAKYKVEAYEYESDDCFFYEGCSYFYNVYEEDGSINTYDGLRSRYNKPEYSTLVYDKNIDKKYLANMEYFSLYWDGEGSSLTRYDGKQVASYYTLNTKYPKYPNLVELNTSTEFTISGNPVTLEEARNYFENIEILDSDERNLANRYSNYLKNYNKYMETIYLKNPDKWVSSLDGYNAWDLMSISRSNFIAGVNGDYTEVGQWSESGGYGVKSSMFPGYIIDYPGEYPRELGVYSGEITDSTWVGMSYSELVSALGKPDSYYLSLIDGNFPVVSYNNIDGMHISFYFYDVTADREGLDDRKDAKRCLEEKNPSVTHALIVNGLWGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5V6X7</accession>
    <name>R5V6X7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Flavoredoxin putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01594</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000167">
        <property type="protein sequence ID" value="CCZ83859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01594"/>
    <dbReference type="EnsemblBacteria" id="CCZ83859">
        <property type="protein sequence ID" value="CCZ83859"/>
        <property type="gene ID" value="BN566_01594"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016646">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-NH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002563">
        <property type="entry name" value="Flavin_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01613">
        <property type="entry name" value="Flavin_Reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavin_Reduct" evidence="1">
        <location>
            <begin position="12"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="188" mass="20456" checksum="C8EF6097E70FDAA9" modified="2013-07-24" version="1">MRKKLDTTGNVFPMPVLMVATYNEDGSVDVMNAAWGTMQESGIVALNLSESHKTTQNILKRKAFTVSFADAAHVAEADYVGMVSGNKEPDKFAKSGLTASKAECVDAPVINEFPICLECEFLAYQDEPYSCGVIGKIVNVTADESVLKDGKVDISLVNAIAFDPYTHGYYKVTERVGEAFQDGKKLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5V799</accession>
    <name>R5V799_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01698</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000177">
        <property type="protein sequence ID" value="CCZ83969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01698"/>
    <dbReference type="EnsemblBacteria" id="CCZ83969">
        <property type="protein sequence ID" value="CCZ83969"/>
        <property type="gene ID" value="BN566_01698"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="175" mass="19251" checksum="94B145C8EA0622C6" modified="2013-07-24" version="1">MWKQLQARLKSEKAVQGVVVLGLLGMLCILASSCFSKPKETDTQQTNHSSEDSVLAAAAYCTELEEQLEQTLSTIQGVGTCDVMITLRSTSIYQYAKDESRSDSSDRVEQKQDYVLIGNGTSEHALVQSIQYPEIQGVVIVCDGGENAVVKEQVYQVAAATLQIKNNQIYVAKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7I3</accession>
    <name>R5V7I3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted flavoproteins</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01770</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000181">
        <property type="protein sequence ID" value="CCZ84044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01770"/>
    <dbReference type="EnsemblBacteria" id="CCZ84044">
        <property type="protein sequence ID" value="CCZ84044"/>
        <property type="gene ID" value="BN566_01770"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004792">
        <property type="entry name" value="BaiN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023166">
        <property type="entry name" value="HI0933-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42887">
        <property type="entry name" value="PTHR42887"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00275">
        <property type="entry name" value="TIGR00275"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="415" mass="46096" checksum="E34A789B344F9069" modified="2013-07-24" version="1">MQKQYHIAVIGGGASGLLAAIAAKIEGGADCSVVILERNDRIGRKILSTGNGRCNLGNTVIQKENYSGSLLEKAFAIFQQAPKTEDYFRQMGLICREEEHRLYPYCNHASAVLDSLRLQVELYGIAVQCDCQIQNLIPKKHGFLLQSDTESYFAEQVIFACGGYAAPALGTDGSAYAMLKDLKIAIRPCTPALCFLKTKTELVRALKGIRLLAKVSLYENGGLKRQERGEVQFTEQALSGICIFNLSAYCDKLSHATYTVALDLLPDWDLEQVYAFLWELYALRGTLSVEEFFTGVFQRRVGLQVLKQCGILQKEPRLIASCTPKEIEQLASFLKAWKFPVIGKGDWKNAQISCGGIISEEVSTNLESIRYPGLYFSGEALDLQGDCGGYNLNWAWASGWWTGTHAAKEWKQKHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7J0</accession>
    <name>R5V7J0_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PALP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_01780</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">The beta subunit is responsible for the synthesis of L-tryptophan from indole and L-serine.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Tetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000183">
        <property type="protein sequence ID" value="CCZ84054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01780"/>
    <dbReference type="EnsemblBacteria" id="CCZ84054">
        <property type="protein sequence ID" value="CCZ84054"/>
        <property type="gene ID" value="BN566_01780"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004834">
        <property type="term" value="F:tryptophan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006316">
        <property type="entry name" value="Trp_synth_b-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48077:SF6">
        <property type="entry name" value="PTHR48077:SF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PALP" evidence="5">
        <location>
            <begin position="8"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ84054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="175" mass="19177" checksum="98796DA95937178F" modified="2013-07-24" version="1">MGEKLRGEADYRFIAVEPASCPSLTRGVYAYDFCDTGKVCPLQKMYTLGSGFMPAPNHAGGLRYHGMSATLSELYDQGLMEATSVEQTAVFAAAEQFARVEGILPAPESSHAIKVAIDEALRCKETGEEKTILFGLTGTGYFDMYAYEKFNDGKMSDYIPTDAELQKSLENLPKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7T6</accession>
    <name>R5V7T6_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PHB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_01899</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000193">
        <property type="protein sequence ID" value="CCZ84174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01899"/>
    <dbReference type="EnsemblBacteria" id="CCZ84174">
        <property type="protein sequence ID" value="CCZ84174"/>
        <property type="gene ID" value="BN566_01899"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03401">
        <property type="entry name" value="SPFH_prohibitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000163">
        <property type="entry name" value="Prohibitin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23222">
        <property type="entry name" value="PTHR23222"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00679">
        <property type="entry name" value="PROHIBITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="SSF117892"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004406790" description="PHB domain-containing protein" evidence="3">
        <location>
            <begin position="34"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="PHB" evidence="4">
        <location>
            <begin position="26"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="271"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="179"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ84174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="290" mass="31223" checksum="53A25CE94B9A91B2" modified="2013-07-24" version="1">MKSLKKATGWIIAGVAALLIIVAATSATTIVPAGHAGVVVTMGSVSNRVLDEGLHFKVPFVQQVVMMNNKIQKTEVASNSVSKDLQTISSSVAINFHITKDASATIYQTIGEQYSDTVLQPAIQEAVKAVTAQYTAEELITKRSAVGDEIGQTLSEKVSDYGILIDKFNIVDFDFSEEFNAAIEQKQVAEQNKLRAETEKEQQIIEAEAAAKQKTIAADADAEATLKKAEAEAAANEKINASLNDKVLRYQQIQKWDGKYPNVVSDSASVLMNLPSDDADSSSTETPTNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>R5V8Y8</accession>
    <name>R5V8Y8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Adenosylcobinamide kinase</fullName>
            <ecNumber evidence="8">2.7.1.156</ecNumber>
            <ecNumber evidence="9">2.7.7.62</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Adenosylcobinamide-phosphate guanylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">BN566_00420</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes ATP-dependent phosphorylation of adenosylcobinamide and addition of GMP to adenosylcobinamide phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenosylcob(III)inamide + ATP = adenosylcob(III)inamide phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15769"/>
            <dbReference type="ChEBI" id="CHEBI:2480"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58502"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.156"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>adenosylcob(III)inamide + GTP = adenosylcob(III)inamide phosphate + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15765"/>
            <dbReference type="ChEBI" id="CHEBI:2480"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:58502"/>
            <dbReference type="EC" id="2.7.1.156"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>adenosylcob(III)inamide phosphate + GTP + H(+) = adenosylcob(III)inamide-GDP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22712"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58502"/>
            <dbReference type="ChEBI" id="CHEBI:60487"/>
            <dbReference type="EC" id="2.7.7.62"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; adenosylcobalamin biosynthesis; adenosylcobalamin from cob(II)yrinate a,c-diamide: step 5/7.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; adenosylcobalamin biosynthesis; adenosylcobalamin from cob(II)yrinate a,c-diamide: step 6/7.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the CobU/CobP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.156" evidence="8"/>
    <dbReference type="EC" id="2.7.7.62" evidence="9"/>
    <dbReference type="EMBL" id="CBAR010000245">
        <property type="protein sequence ID" value="CCZ84559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00420"/>
    <dbReference type="EnsemblBacteria" id="CCZ84559">
        <property type="protein sequence ID" value="CCZ84559"/>
        <property type="gene ID" value="BN566_00420"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00236"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043752">
        <property type="term" value="F:adenosylcobinamide kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008820">
        <property type="term" value="F:cobinamide phosphate guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003203">
        <property type="entry name" value="Cobinamide_kinase/P_G-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34848">
        <property type="entry name" value="PTHR34848"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02283">
        <property type="entry name" value="CobU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006135">
        <property type="entry name" value="CobU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="17" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="12 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 18" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="GMP-histidine intermediate" evidence="16">
        <location>
            <position position="49"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="7"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="50"/>
            <end position="53"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00003889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00005159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00007490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00012523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR006135-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR006135-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CCZ84559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="180" mass="19717" checksum="659A901AECFCBEEB" modified="2013-07-24" version="1">MQILISGGANSGKSSLAEQLAVAQSFQPRYYFATMQVWDAECARRIERHRQQRAGKDFQTVEVPTNLLETISTLPQGGCGLLECVSNLLSNEQFGGSQEDPVTAILDGMAALQKKLDAVVIVTNEVFTDRVPNDVAMQQYLQNLGNINQVLASRSDVVIEVCSGIPICWKGASLFHEIML</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5V960</accession>
    <name>R5V960_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_00497</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000257">
        <property type="protein sequence ID" value="CCZ84644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00497"/>
    <dbReference type="EnsemblBacteria" id="CCZ84644">
        <property type="protein sequence ID" value="CCZ84644"/>
        <property type="gene ID" value="BN566_00497"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01041">
        <property type="entry name" value="Rubrerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009040">
        <property type="entry name" value="Ferritin-like_diiron"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024934">
        <property type="entry name" value="Rubredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003251">
        <property type="entry name" value="Rubrerythrin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02915">
        <property type="entry name" value="Rubrerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50905">
        <property type="entry name" value="FERRITIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50903">
        <property type="entry name" value="RUBREDOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Ferritin-like diiron" evidence="5">
        <location>
            <begin position="3"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Rubredoxin-like" evidence="4">
        <location>
            <begin position="142"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ84644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="180" mass="20318" checksum="F86E31D515DED7A1" modified="2013-07-24" version="1">MKPLKGSRTEANLMAAFAGETQARTKYTYYASQAKKDGYVQISKIFEETAANEKEHAKIWFKLLHGGAMPATTENLKDAAAGEHFEWADMYREFAEQARQEGFDQIAALFEAVGKIEKTHEERFRKLLANVEGGYVFSKDGDTIWECANCGHIHIGKQAPEVCPVCNHPQSYFQVKAENY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>R5V976</accession>
    <name>R5V976_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">4'-phosphopantetheinyl transferase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00517</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000267">
        <property type="protein sequence ID" value="CCZ84664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00517"/>
    <dbReference type="EnsemblBacteria" id="CCZ84664">
        <property type="protein sequence ID" value="CCZ84664"/>
        <property type="gene ID" value="BN566_00517"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008897">
        <property type="term" value="F:holo-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008278">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037143">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01648">
        <property type="entry name" value="ACPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56214">
        <property type="entry name" value="SSF56214"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ACPS" evidence="2">
        <location>
            <begin position="109"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="232" mass="26118" checksum="CFC296EF71FE58E7" modified="2013-07-24" version="1">MINRKCRKKGVETLIQQVQTTAANPVESEQIDLPEVVLQPLPMNAKVAEQHHFAHGLLEEMLFKRGIFKPYFMGEGKHGKPFLLEHPQIQFNISHCKNAVLCGIGSKPLGVDVETIRPMRERVLHRVLTASEQQEILQSESPELCFLRFWTLKESFVKALGVGISYPMQTLAFSLREPLATEGMQCHVRQGSGEILVPMQEYAAAGAGAGYRFFQGIWEQKQVFSVCIPKAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5V9A0</accession>
    <name>R5V9A0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00547</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000267">
        <property type="protein sequence ID" value="CCZ84694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84694">
        <property type="protein sequence ID" value="CCZ84694"/>
        <property type="gene ID" value="BN566_00547"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004395020" evidence="1">
        <location>
            <begin position="32"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="233" mass="25107" checksum="94A1FF307970DF95" modified="2013-07-24" version="1">MKKLFACTTAIVLLAGGLVGCGSNGTGDSKVLETTRAVSEDGWGSTVSRMGTDTGNFTKLRCGMSGVDVADLGYGSKESDQDFTGNNKAQTMDHILHFYADGVISFDYSGTPAEMGTDPDTKQPYSAYVDISVNGDTGELYAARYMIGVSEEDADTAAALADALFEQYDNGSGEQTSEHGYLIPNCDTFNGNLIIYYGANTDKTQNAFIFRIYNEDYNNAPDFYQDGNLKDNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5VGR8</accession>
    <name>R5VGR8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00581</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000007">
        <property type="protein sequence ID" value="CCZ82776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82776">
        <property type="protein sequence ID" value="CCZ82776"/>
        <property type="gene ID" value="BN566_00581"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021215">
        <property type="entry name" value="DUF2752"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10825">
        <property type="entry name" value="DUF2752"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="17"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="143" mass="16571" checksum="5132E748B9129040" modified="2013-07-24" version="1">MLQQQIKQKTWHAAEKWIWLFLPIGLLMGVLLGRHLIFNLVQLYFPPCFFYTVTHFYCPGCGNTRTVLALLHGHPLQALHNNAAFCYLLVVLVLLYLQKVLRCFGKPVQLLPKGNWFPVVSVCICMAYCLIRNFIPWMQPLPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5VGU5</accession>
    <name>R5VGU5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BH3136 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00601</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000008">
        <property type="protein sequence ID" value="CCZ82796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00601"/>
    <dbReference type="EnsemblBacteria" id="CCZ82796">
        <property type="protein sequence ID" value="CCZ82796"/>
        <property type="gene ID" value="BN566_00601"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012156">
        <property type="entry name" value="Cold_shock_CspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010718">
        <property type="entry name" value="DUF1294"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06961">
        <property type="entry name" value="DUF1294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002599">
        <property type="entry name" value="Cold_shock_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="89" mass="10077" checksum="FAE3F345C2CFAEF5" modified="2013-07-24" version="1">MGKWLLLLWLGLASLLLFGMMGADKKKAKRGQWRIPEKTLFLVAICGGAVGGTLGMYQFHHKTKHRSFQIGFPFLAVLQLLLLGYLWMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5VH37</accession>
    <name>R5VH37_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Precorrin-3B C(17)-methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_00678</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000020">
        <property type="protein sequence ID" value="CCZ82876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00678"/>
    <dbReference type="EnsemblBacteria" id="CCZ82876">
        <property type="protein sequence ID" value="CCZ82876"/>
        <property type="gene ID" value="BN566_00678"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11646">
        <property type="entry name" value="Precorrin_3B_C17_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006363">
        <property type="entry name" value="Cbl_synth_CobJ/CibH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="SSF53790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01466">
        <property type="entry name" value="cobJ_cbiH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TP_methylase" evidence="5">
        <location>
            <begin position="4"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ82876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="244" mass="26337" checksum="4C2A6FDF14281E43" modified="2013-07-24" version="1">MQPTIYAVGFGPGDVLHMTTQAVSVLETVDVIVGYTTYIQLLQEQFPDKQFRSTPMRQEIDRCKLAVSIALEGKTVALVSSGDSGIYGMTGILLEVIAAEQADVEVIAVPGVTAASAAAAILGAPLMHDFAVISLSDLMTPLSLIQKRITCAAEADFVICLYNPKSKKRTTYLADAADRIAAYRTPDTPVGIVRNAGRPDQTAWFTTLSKLKEEPVDMFCVVLIGNSQTYLKNGKMITPRGYNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>R5VHH4</accession>
    <name>R5VHH4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Beta sliding clamp</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BN566_00103</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Confers DNA tethering and processivity to DNA polymerases and other proteins. Acts as a clamp, forming a ring around DNA (a reaction catalyzed by the clamp-loading complex) which diffuses in an ATP-independent manner freely and bidirectionally along dsDNA. Initially characterized for its ability to contact the catalytic subunit of DNA polymerase III (Pol III), a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria; Pol III exhibits 3'-5' exonuclease proofreading activity. The beta chain is required for initiation of replication as well as for processivity of DNA replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Forms a ring-shaped head-to-tail homodimer around DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the beta sliding clamp family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000035">
        <property type="protein sequence ID" value="CCZ82981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00103"/>
    <dbReference type="EnsemblBacteria" id="CCZ82981">
        <property type="protein sequence ID" value="CCZ82981"/>
        <property type="gene ID" value="BN566_00103"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009360">
        <property type="term" value="C:DNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00140">
        <property type="entry name" value="beta_clamp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001001">
        <property type="entry name" value="DNA_polIII_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022635">
        <property type="entry name" value="DNA_polIII_beta_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022637">
        <property type="entry name" value="DNA_polIII_beta_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022634">
        <property type="entry name" value="DNA_polIII_beta_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30478">
        <property type="entry name" value="PTHR30478"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00712">
        <property type="entry name" value="DNA_pol3_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02767">
        <property type="entry name" value="DNA_pol3_beta_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02768">
        <property type="entry name" value="DNA_pol3_beta_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000804">
        <property type="entry name" value="DNA_pol_III_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00480">
        <property type="entry name" value="POL3Bc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00663">
        <property type="entry name" value="dnan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 11" id="KW-0235">DNA replication</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 11" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="7 11" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA_pol3_beta" evidence="12">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="DNA_pol3_beta_2" evidence="13">
        <location>
            <begin position="129"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="DNA_pol3_beta_3" evidence="14">
        <location>
            <begin position="246"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR000804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CCZ82981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="366" mass="41010" checksum="E06D05906D1CC618" modified="2013-07-24" version="1">MQFTCDRQALYQAVTYVSKGVAQKSTIPALEGIRMQLRPGQLQLTGYDLEFGIQTNLPVDTHDTGEFVIHARLFSEALRRFSGNTVLLEIDSRLAVTMHCEATEFHISAMSAEDYPSLPELEMEDGLTLEQGTLRSMIHQTSYATSLNENKPVFTGELFETIGDELRVVAIDGYRLAIRTEKIATKVPYHFVVPKRTLLEVASMLKDDAEAPCELTATRNHIAFSFNGYLVFSRLLEGEFHNYQSSIPSGYETEVILQTADLISCLERCSLLINSKFNAPIRCTFSDNQLYVHCETGIGKIHDTIPAQVKGPEVTIGFNNRYLLEAAKASDCDQIRLQLTGGNHVAKMVPMQGENFIFLLMPIQLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5VHL5</accession>
    <name>R5VHL5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Branched-chain amino acid transport protein (AzlD)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00789</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000036">
        <property type="protein sequence ID" value="CCZ82996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82996">
        <property type="protein sequence ID" value="CCZ82996"/>
        <property type="gene ID" value="BN566_00789"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008407">
        <property type="entry name" value="Brnchd-chn_aa_trnsp_AzlD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05437">
        <property type="entry name" value="AzlD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="102" mass="11240" checksum="6A1A5A90C3EEC4C6" modified="2013-07-24" version="1">MRIICSILVMAVVTYLIRMLPMTIFRKKIQSRFLQDFLYYIPYAILSAMTIPAVFYATGDFPSAIAATVIAVVLAWNGLPLIVVALAASGTALLASLIEQWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5VHR8</accession>
    <name>R5VHR8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Haloacid dehalogenase superfamily subfamily IA variant 3 with third motif having DD or ED/haloacid dehalogenase superfamily subfamily IA variant 1 with third motif having Dx(3-4)D or Dx(3-4)E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00834</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000040">
        <property type="protein sequence ID" value="CCZ83041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00834"/>
    <dbReference type="EnsemblBacteria" id="CCZ83041">
        <property type="protein sequence ID" value="CCZ83041"/>
        <property type="gene ID" value="BN566_00834"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="226" mass="25389" checksum="E4F15112B82ADA20" modified="2013-07-24" version="1">MEHHTLGCVIFDLDGTLLDSMNVWSQIDRNLLNHYGKTAPDDLTEQVKKMTIQQSAQYFIDRFQLPCEPEEFIALVQSMAADAYRNTLQLKSGVFPLLDWLDARNVPYCVATATYPDLAEAALKRLQVWERIAFLMTEQEVGVGKSQPTIYRQAASRMGCGKRQTVVVEDAVHAIETAATDGFFTVGVYEPTLSEMDWNTIQARATVSVKDLQKLLAEIQSGRIPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5VI66</accession>
    <name>R5VI66_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Fe2+ transport system protein A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01032</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000082">
        <property type="protein sequence ID" value="CCZ83251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01032"/>
    <dbReference type="EnsemblBacteria" id="CCZ83251">
        <property type="protein sequence ID" value="CCZ83251"/>
        <property type="gene ID" value="BN566_01032"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007167">
        <property type="entry name" value="Fe-transptr_FeoA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038157">
        <property type="entry name" value="FeoA_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008988">
        <property type="entry name" value="Transcriptional_repressor_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04023">
        <property type="entry name" value="FeoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00899">
        <property type="entry name" value="FeoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50037">
        <property type="entry name" value="SSF50037"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FeoA" evidence="2">
        <location>
            <begin position="2"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="73" mass="8169" checksum="5921FF448114D9D9" modified="2013-07-24" version="1">MATLREAKIGETVTVKRIHGEGALKRRIMDMGITKGVQIYVRKVAPLGDPIEVTVRDYELSLRKADAEMIETV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>R5VIC5</accession>
    <name>R5VIC5_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glycogen synthase</fullName>
            <ecNumber evidence="7">2.4.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Starch [bacterial glycogen] synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">glgA</name>
        <name evidence="10" type="ORF">BN566_01097</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 7">Synthesizes alpha-1,4-glucan chains using ADP-glucose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>[(1-&gt;4)-alpha-D-glucosyl](n) + ADP-alpha-D-glucose = [(1-&gt;4)-alpha-D-glucosyl](n+1) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18189"/>
            <dbReference type="Rhea" id="RHEA-COMP:9584"/>
            <dbReference type="Rhea" id="RHEA-COMP:9587"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15444"/>
            <dbReference type="ChEBI" id="CHEBI:57498"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.4.1.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Glycan biosynthesis; glycogen biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the glycosyltransferase 1 family. Bacterial/plant glycogen synthase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.21" evidence="7"/>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01097"/>
    <dbReference type="EnsemblBacteria" id="CCZ83316">
        <property type="protein sequence ID" value="CCZ83316"/>
        <property type="gene ID" value="BN566_01097"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00164"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033201">
        <property type="term" value="F:alpha-1,4-glucan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004373">
        <property type="term" value="F:glycogen (starch) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009011">
        <property type="term" value="F:starch synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00484">
        <property type="entry name" value="Glycogen_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011835">
        <property type="entry name" value="GS/SS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013534">
        <property type="entry name" value="Starch_synth_cat_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08323">
        <property type="entry name" value="Glyco_transf_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02095">
        <property type="entry name" value="glgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0320">Glycogen biosynthesis</keyword>
    <keyword evidence="4 7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_transf_5" evidence="9">
        <location>
            <begin position="2"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="8">
        <location>
            <begin position="287"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ83316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="473" mass="54409" checksum="ED856490A62CFE09" modified="2013-07-24" version="1">MKILYAASEALPFAASGGLADVMGALPKAMVQQQCDCRVVLPLYRSVKPAVRNQMTFLKDFDVEIGWRKQYCGVYTIQRNGVIYYLLDNPYYFMRDGMYGFYDDAERFIFFSRAVLELLSEIAFFPDLIHCNDWQTAMIPVYYDIFYKYRQGYEKIKTIYTIHNIQYQGVYGKEIISELMGIPTYYTNLLDYDGCVNLMKGAIETADCVTTVSPTYAKEILDPWFSYGMDRELVRRQDGVRGILNGIDVDLYNPETDPDIKEHYQVNRRTGKKACKTDLLEEMKWEDNGQPVIGIVTRFVAHKGIHLIQYAFQEMLELGCRFVILGSGEKIFEDFFREMQLQHPEQVSVHIGFLPQMAKRIYAGADIFLMPSQNEPCGLAQMVAMRYGTIPIVRETGGLKDTVTDAGTIGGNGFTFKTYDAMDMLDAVKRACALYQEKRKWNALVKRAMLCDFSWNRSAKDYLDLYQEITGQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5VIJ8</accession>
    <name>R5VIJ8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ADP-ribosylglycohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01157</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000093">
        <property type="protein sequence ID" value="CCZ83381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01157"/>
    <dbReference type="EnsemblBacteria" id="CCZ83381">
        <property type="protein sequence ID" value="CCZ83381"/>
        <property type="gene ID" value="BN566_01157"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4080.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005502">
        <property type="entry name" value="Ribosyl_crysJ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036705">
        <property type="entry name" value="Ribosyl_crysJ1_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03747">
        <property type="entry name" value="ADP_ribosyl_GH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101478">
        <property type="entry name" value="SSF101478"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="372" mass="40994" checksum="DCFA5EFE168555F4" modified="2013-07-24" version="1">MDDFSTGYICYMPSPCPLTGFGAWVVTSRYEIMNETKNYITVDDIGNGKVWIKNTVLDSSKCRTCFYDQFICERTQTKTMMLGAICGDVAGSIYEHNNIKYIPDKDRLIDHYARFTDDSVLTLAVAEGIMNGMRSLQKVKDFNSDDEPVFADALTKSIHKWGNLYPYAGYGGAFRRWLASENPEPYNSWGNGSAMRASYAGWIAQSLEDAEFLATASASVTHNHPEGIKGAVAIAGSIYLLRNSDPADAKETVKRYVSKLYDLDLTLDEIRPVYHFDVSCQGSVPQAIQAFLEGESFSEVLALAISIGGDSDTIAAMAGSLAEVIYPIPDGMRDRVLKRLDYTFKSCLAGTIDFLIDNLNKISGLESSAISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5VIL4</accession>
    <name>R5VIL4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ATPase associated with various cellular activities AAA_3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01167</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000094">
        <property type="protein sequence ID" value="CCZ83391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01167"/>
    <dbReference type="EnsemblBacteria" id="CCZ83391">
        <property type="protein sequence ID" value="CCZ83391"/>
        <property type="gene ID" value="BN566_01167"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011703">
        <property type="entry name" value="ATPase_AAA-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041628">
        <property type="entry name" value="ChlI/MoxR_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07726">
        <property type="entry name" value="AAA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17863">
        <property type="entry name" value="AAA_lid_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_3" evidence="1">
        <location>
            <begin position="43"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="AAA_lid_2" evidence="2">
        <location>
            <begin position="234"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="321" mass="35995" checksum="C3C909B5AB45B777" modified="2013-07-24" version="1">MQTNPQEYVQQMNRIIAEVKKAVVGKDVLIAKILLAVLANGNVLMEDMPGVGKTTMALAFSRALSLSYKRMQFTPDVLPSDLTGFSIYQKSTEKFVFQPGALFCQLFLADEINRTSSKTQSALLEAMEEHSVTIDGRTYQLPQPHLVIATQNPIGSVGTQMLPESQMDRFMFRLEMGYPNLEDEMQILRTRHDGNPLETVQPVVDSNTLLQMQQAVRSVYVDDKIYYYIASLVQATRNHPDVLLGVSPRGTLALTDAAKGMAFMRGRNYVVPEDVTAIFEDGCNHRILLKNHTNHTQAVRQLIQELIQTVPVPKPTAAQRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJ64</accession>
    <name>R5VJ64_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Hemolysins and related proteins containing CBS domains</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BN566_01300</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0053 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000121">
        <property type="protein sequence ID" value="CCZ83531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01300"/>
    <dbReference type="EnsemblBacteria" id="CCZ83531">
        <property type="protein sequence ID" value="CCZ83531"/>
        <property type="gene ID" value="BN566_01300"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04590">
        <property type="entry name" value="CBS_pair_CorC_HlyC_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002550">
        <property type="entry name" value="CNNM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044751">
        <property type="entry name" value="Ion_transp-like_CBS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005170">
        <property type="entry name" value="Transptr-assoc_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03471">
        <property type="entry name" value="CorC_HlyC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01595">
        <property type="entry name" value="DUF21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01091">
        <property type="entry name" value="CorC_HlyC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="SSF56176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51846">
        <property type="entry name" value="CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0129">CBS domain</keyword>
    <keyword evidence="7 9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="97"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="135"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="CNNM transmembrane" evidence="12">
        <location>
            <begin position="1"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="11">
        <location>
            <begin position="281"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CCZ83531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="433" mass="47759" checksum="AAC8A6E9DE00E7D1" modified="2013-07-24" version="1">MIGQILLQLILIALNAIFACAEIAVISSNETRIDKLAEQGNKPAKRLQALQNRPAQFLATIQVAITLSGFLGSAFAADHFSEALVQLLVSWGVAIPQATLQTISVVLITILLSFLTLVFGELVPKRIAMRKAESIALGISGMIQILAKLCSPLVWLLTAATNGVLRLFRIDPNAEEEDISEENIRMMADAGSEKGVIAAEENEMIQNVFAFNDWTAEEIMTHRTELQLLWERDSLDSWKQTIRQTPHRYYPICGEHTDDILGVLDTRKLFAVAEQTKECVMKTAVVPAFFIPSTAKANDVFHSMRKAGQSYAIVLDEFGGTDGVLTMLDLMERLVGSLDEAADASELQEENGIYRIAGNCELERMEETLRMQLPETDATTAGGWIVEQLGRIPHTGETLLLHGYQFQIEKANPRKVLLFSATPHTESASTAAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJG5</accession>
    <name>R5VJG5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phage terminase large subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01407</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000148">
        <property type="protein sequence ID" value="CCZ83671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01407"/>
    <dbReference type="EnsemblBacteria" id="CCZ83671">
        <property type="protein sequence ID" value="CCZ83671"/>
        <property type="gene ID" value="BN566_01407"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005021">
        <property type="entry name" value="Terminase_largesu_Lambdalike"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41287">
        <property type="entry name" value="PTHR41287"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03354">
        <property type="entry name" value="Terminase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="578" mass="66806" checksum="74DEBA9E8C10EFB7" modified="2013-07-24" version="1">MATKQFKQIAIQYAEDVVTGKIIAGNNLLECKRFLEDLKREDLELHTKEPDLVCNIIERFMVHKQGESLKGEPLMNTPMLLQPWQVFTVYNLVGFYYTGTKERRYKEAFIFIPRKSGKTMFIAALAFALAILERRSGSIIYIVAASQKQACESFNDILYTLRYREMIDDFRVLNNNAEHSISYQFTDANGRPNGSIRIEALASNPDAQDSFNCNIAIADEVHAFKKSAQYNRFKEAMKAYTNKLMIGITTAGDNINSFCYRRLEYAKKVLNGTVKDDTLFCFVSQAEQDEHGQVDYTSPIQHEKANPSYGVTIRPADILQESLQAQNDPQQRKDFLSRSLNIYTSAMRAYFDLSEFRTSDNKYNWTIEDLLRMSIDWYGGADLSRMYDLTAGALYGHYAKEDVDIIITHAFFPVTMAAKKADEDEIPLFGWADDGLLTMCNSPTVNAADVVNWFVSMRRHGFKIKQIGHDRKFAREYFIGMKQAKFNIIDQPQYYYLKSEGFRHIEQRAKDGKLYYLHNEAFEYCVENVSAIEKTDDMIQYEKIGETNRIDLFDASVFACVRYLQSMERKQKAKDWWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJJ3</accession>
    <name>R5VJJ3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">N5-carboxyaminoimidazole ribonucleotide mutase</fullName>
            <shortName evidence="3 4">N5-CAIR mutase</shortName>
            <ecNumber evidence="3 4">5.4.99.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">5-(carboxyamino)imidazole ribonucleotide mutase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">purE</name>
        <name evidence="7" type="ORF">BN566_01442</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 4">Catalyzes the conversion of N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) to 4-carboxy-5-aminoimidazole ribonucleotide (CAIR).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole + H(+) = 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate</text>
            <dbReference type="Rhea" id="RHEA:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58730"/>
            <dbReference type="ChEBI" id="CHEBI:77657"/>
            <dbReference type="EC" id="5.4.99.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 4">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route): step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AIR carboxylase family. Class I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.18" evidence="3 4"/>
    <dbReference type="EMBL" id="CBAR010000154">
        <property type="protein sequence ID" value="CCZ83706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01442"/>
    <dbReference type="EnsemblBacteria" id="CCZ83706">
        <property type="protein sequence ID" value="CCZ83706"/>
        <property type="gene ID" value="BN566_01442"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00943"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034023">
        <property type="term" value="F:5-(carboxyamino)imidazole ribonucleotide mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01929">
        <property type="entry name" value="PurE_classI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033747">
        <property type="entry name" value="PurE_ClassI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000031">
        <property type="entry name" value="PurE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024694">
        <property type="entry name" value="PurE_prokaryotes"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00731">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001338">
        <property type="entry name" value="AIR_carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01001">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01162">
        <property type="entry name" value="purE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="1 3 4" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIRC" evidence="6">
        <location>
            <begin position="3"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR001338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001338-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ83706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="165" mass="16883" checksum="8926808A52BE1A9D" modified="2013-07-24" version="1">MSKKVAIIMGSDSDLPVVKAAISELKAFGVPSEVHVMSAHRTPELVAEFATNAKENGFGVIIAAAGKAAHLAGVIAGHTTLPVIGIPIKSSTLDGLDALLATVQMPTGIPVATVAIDGAANAAILAVQMLALSDDALAEQLVQRKKTMAEGVVEKEKKLLAQLEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJM4</accession>
    <name>R5VJM4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Indolepyruvate oxidoreductase subunit IorA</fullName>
            <shortName evidence="14">IOR</shortName>
            <ecNumber evidence="3 14">1.2.7.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 14">Indolepyruvate ferredoxin oxidoreductase subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">BN566_01476</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 14">Catalyzes the ferredoxin-dependent oxidative decarboxylation of arylpyruvates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>CoA + indole-3-pyruvate + 2 oxidized [2Fe-2S]-[ferredoxin] = (indol-3-yl)acetyl-CoA + CO2 + H(+) + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:12645"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17640"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57271"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="EC" id="1.2.7.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14 15">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="14 15">Binds 2 [4Fe-4S] clusters. In this family the first cluster has a non-standard and varying [4Fe-4S] binding motif CX(2)CX(2)CX(4-5)CP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterodimer of the IorA and IorB subunits.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.8" evidence="3 14"/>
    <dbReference type="EMBL" id="CBAR010000160">
        <property type="protein sequence ID" value="CCZ83741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01476"/>
    <dbReference type="EnsemblBacteria" id="CCZ83741">
        <property type="protein sequence ID" value="CCZ83741"/>
        <property type="gene ID" value="BN566_01476"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043805">
        <property type="term" value="F:indolepyruvate ferredoxin oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07034">
        <property type="entry name" value="TPP_PYR_PFOR_IOR-alpha_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045025">
        <property type="entry name" value="HACL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017721">
        <property type="entry name" value="IorA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002880">
        <property type="entry name" value="Pyrv_Fd/Flavodoxin_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43710">
        <property type="entry name" value="PTHR43710"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01855">
        <property type="entry name" value="POR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006439">
        <property type="entry name" value="Indolepyruvate_ferr_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03336">
        <property type="entry name" value="IOR_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 14 15" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="8 14" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10 14 15" id="KW-0408">Iron</keyword>
    <keyword evidence="11 14 15" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="16">
        <location>
            <begin position="549"/>
            <end position="578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00033657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR006439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR006439-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CCZ83741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="579" mass="62786" checksum="65BF3B8EED489E1D" modified="2013-07-24" version="1">MKKLLLGNAAVARGAYEAGVRVVSSYPGTPSTEITEQIVPYKEIYAEWAPNEKVAAEVAIGASIGGARAMSCMKHVGLNVMADPVFTVSYSGVNGGLVFCVADDPGMHSSQNEQDSRHYAEAAKIPMLEPSDSEECKNFTKLAYTISETFDAPAFLRLSTRVSHSQSLVELEEPEEVALKPYEKDAAKYVMMPANAIKRHVVVEERMRKLAEYAETCPINRVESKGNKIGVISAGIAYLYAKEALGDQVDYCKLGMIYPMPKQMLLDFAAQHETVYVIEELDPFIETYCRSLGISVIGKEAFSLLGEYTPHQIRKVVLGEEEPAYQVPGEPIPVRPPVMCAGCPHRGVFYVLHKLKLQVSGDIGCYTLGAVAPLNSMDTTICMGASVGVAMGMAKARGNDFAKKTVSVIGDSTFIHSGITGLIDVVYNKGINTIIILDNSITGMTGHQDNPTTGYTIRKEVTKQVNLVTLCKSVGVERIAIVDPFDVKQMEAVVKQEIAAEEPSVIIAQRPCALLKTVKYTGCCEISTDCKKCKLCMKLGCPAISFDGTKPTINPTQCNGCGLCLNVCPFGCIHKKEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJN9</accession>
    <name>R5VJN9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">dITP/XTP pyrophosphatase</fullName>
            <ecNumber evidence="6">3.6.1.66</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Non-canonical purine NTP pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Non-standard purine NTP pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Nucleoside-triphosphate diphosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Nucleoside-triphosphate pyrophosphatase</fullName>
            <shortName evidence="6">NTPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_01496</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP. Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>H2O + ITP = diphosphate + H(+) + IMP</text>
            <dbReference type="Rhea" id="RHEA:29399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>H2O + XTP = diphosphate + H(+) + XMP</text>
            <dbReference type="Rhea" id="RHEA:28610"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:61314"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>dITP + H2O = dIMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28342"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61194"/>
            <dbReference type="ChEBI" id="CHEBI:61382"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="6">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the HAM1 NTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.66" evidence="6"/>
    <dbReference type="EMBL" id="CBAR010000161">
        <property type="protein sequence ID" value="CCZ83761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01496"/>
    <dbReference type="EnsemblBacteria" id="CCZ83761">
        <property type="protein sequence ID" value="CCZ83761"/>
        <property type="gene ID" value="BN566_01496"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035870">
        <property type="term" value="F:dITP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036220">
        <property type="term" value="F:ITP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:nucleoside-triphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036222">
        <property type="term" value="F:XTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009146">
        <property type="term" value="P:purine nucleoside triphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00515">
        <property type="entry name" value="HAM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01405">
        <property type="entry name" value="Non_canon_purine_NTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020922">
        <property type="entry name" value="dITP/XTP_pyrophosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002637">
        <property type="entry name" value="Ham1p-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11067">
        <property type="entry name" value="PTHR11067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01725">
        <property type="entry name" value="Ham1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="SSF52972"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00042">
        <property type="entry name" value="TIGR00042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="10"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="155"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="181"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="72"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="196" mass="21129" checksum="0FC18783509F12AC" modified="2013-07-24" version="1">MQKETLILASNNAGKLREMREILEPFGLEVRSQKEAGFTAEVAETGTTFQENAIIKAKAVQEALHCAVLADDSGLMIDALDGAPGVYSHRFAGEQATDEDRCNLVLEKLQGVPTEQRTARFVCAICYLTADGEQHCFAGVCEGIIGTEPKGENGFGYDPVFVVGNKTMAEMTEAEKNAISHRGKALAALQAYLKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5VK14</accession>
    <name>R5VK14_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_01615</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000169">
        <property type="protein sequence ID" value="CCZ83886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01615"/>
    <dbReference type="EnsemblBacteria" id="CCZ83886">
        <property type="protein sequence ID" value="CCZ83886"/>
        <property type="gene ID" value="BN566_01615"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="5"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="36"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="284" mass="31998" checksum="42B67B1252450FB3" modified="2013-07-24" version="1">MQYAIEIKNLCKQYPKFALQSVSFTLPMGYIMGFIGENGAGKTTVMKSMLHLVRPDSGTVLLLGQDATKENAALKQQIGVVLDTANFPQSFTTQDVHQMLKRVYSNWDGALYQQYLEQFHLPEKKKFKEFSMGMKKKLAVAAALAHHPKLLILDEVTAGLDPMVREEILDVFRDFVSDGEHSILISSHIISDLEKICDYITFLHEGTVLFSMERDTLLDTYCMVQCTAEDAEALMPEAICGRRETPYHVELLCKRDLLPSAFEAEPANIEDIMVFLTKYAVGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>R5VKP7</accession>
    <name>R5VKP7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA topoisomerase (ATP-hydrolyzing)</fullName>
            <ecNumber evidence="2">5.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_01812</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="2"/>
    <dbReference type="EMBL" id="CBAR010000186">
        <property type="protein sequence ID" value="CCZ84086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01812"/>
    <dbReference type="EnsemblBacteria" id="CCZ84086">
        <property type="protein sequence ID" value="CCZ84086"/>
        <property type="gene ID" value="BN566_01812"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.670">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002288">
        <property type="entry name" value="DNA_gyrase_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001241">
        <property type="entry name" value="Topo_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013760">
        <property type="entry name" value="Topo_IIA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013759">
        <property type="entry name" value="Topo_IIA_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013506">
        <property type="entry name" value="Topo_IIA_bsu_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018522">
        <property type="entry name" value="TopoIIA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00204">
        <property type="entry name" value="DNA_gyraseB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00986">
        <property type="entry name" value="DNA_gyraseB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00433">
        <property type="entry name" value="TOP2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56719">
        <property type="entry name" value="SSF56719"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00177">
        <property type="entry name" value="TOPOISOMERASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Toprim" evidence="4">
        <location>
            <begin position="433"/>
            <end position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ84086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="659" mass="75147" checksum="80094821E8C469BA" modified="2013-07-24" version="1">MAKKEIYNNESITMLKGPDKVRKRPGVIFGSDGVDGCAHSVFEIVSNSIDEARSGFGDKIIVTQYNDHSITVEDFGRGCPVDFNSKENRYNWELVFCELYAGGKYNADGDNYAYSLGLNGLGLCATQFSSAYMDVEIYRDGFCYELHFEKGINIGGLKKTPVKTKKTGTKIHWLPDLEVFTEISIPSEYFRDVLKRQAVVNPNLLFLYRHQQEDGSMEELRFLYENGIADYVQELAGETALTSIQTWDCQREGRDREDKPDYKVKLSVSFTFSNRVQRIEYYHNSSFLEHGGSPERAVKQAFVSQIDSYLKQNGKYLKNESKLTFADVEDCLILVSSSFSTQTSYENQTKKAITNKFIYEAMTEFLKHQLEIYFIENPDEAVKIAEQVLINKRSRENAEKTRLNIKKNLAGNIDLVNRVEKFVDCRSKDVSRRELYIVEGDSALGSVKMARDAEFQAVIPVRGKILNCLKADYNRIFKNEIITDIIKLLGCGVEVKTKANKELSMFHLDGLRWNKIVICTDADVDGFQIRTLILTMLYRLTPTLIENGYVYIAESPLFEITTKKRTYFAYTEKEKVEILQKIGKEPATLQRSKGLGENEADMMALTTMNPETRRLIRVTPAGVEETAAMFDLLLGDDLQGRKQFIAEYGHAYLELADIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5VKU4</accession>
    <name>R5VKU4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01871</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000192">
        <property type="protein sequence ID" value="CCZ84146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01871"/>
    <dbReference type="EnsemblBacteria" id="CCZ84146">
        <property type="protein sequence ID" value="CCZ84146"/>
        <property type="gene ID" value="BN566_01871"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005182">
        <property type="entry name" value="DUF304"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03703">
        <property type="entry name" value="bPH_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="220"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="348"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="380"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="bPH_2" evidence="2">
        <location>
            <begin position="63"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="bPH_2" evidence="2">
        <location>
            <begin position="243"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="488" mass="56511" checksum="A406D560B8EA56EC" modified="2013-07-24" version="1">MLHEHPIKILRYTAKNLWLLIFPLLRSAHFFPFSAKEIAAWLHGAWFDLLICVLILTYGAIRWYFCGFMYNTEVIRRQSGILLKRETEIPCSSITSVTEEHAFFLRPFGAVRVSIHTGAEDIAGADMQLVLYKADLAAIRQVIPLLRPASDVKMHRPHTSRLLLFSFLFSSSLSGAVYAAAIFFQGGRITRDLLEELQAEELLMTLTDQAATYFKGIPRLAITIGILILSAWLISFIGNLLRYSRFRFYADRKAFSVHRGLISPYRYRLRRGAVNYIDIRQNLMMKLLHLMSVHISCPGYGNQKNELPVLMPLLQKSHADRFLQPLLKQQPNVIGKNKPKQPFNLFRFWLFIWQPLFLLGIVLVLIALARYFFYPIYVRFSFLGWMLVFPCVWFLLIRIVSAMRTRMWLEDGKLYLHYSSGFVFHTIVAPATNIASIQVTRTKTGRKCGLCNVFFYLQGKTMHRHKLTGVSESVWQQILAELEQMKDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VL25</accession>
    <name>R5VL25_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00180</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000196">
        <property type="protein sequence ID" value="CCZ84241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84241">
        <property type="protein sequence ID" value="CCZ84241"/>
        <property type="gene ID" value="BN566_00180"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="259" mass="30635" checksum="667B1DA23890F975" modified="2013-07-24" version="1">MQEQILLRCQEVNYKFSTHIVLWMNKKIQTICKMGLSLRSKNELHNALYTENQYIFGGTKYDKIKFKIYNNNYLTTGINEYTISDIFADIESYYATDKEFNMELNKKLYSITKEHFSYYDIKIIFRVISELLNEIGTCIYIDENRTVKQYNSIAIPYVQNLCISHKMPSRQEFEWIFAYAFTSTSDQWDGYSKLDFSTDKFGNYGLKLNNKNFLISILMDALLDDEIIKITKEIFCDIDIATLIQCIKFIKEILLCLEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5VL68</accession>
    <name>R5VL68_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dehydrogenases with different specificities (Related to short-chain alcohol dehydrogenases)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000208">
        <property type="protein sequence ID" value="CCZ84291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00030"/>
    <dbReference type="EnsemblBacteria" id="CCZ84291">
        <property type="protein sequence ID" value="CCZ84291"/>
        <property type="gene ID" value="BN566_00030"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008202">
        <property type="term" value="P:steroid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0753">Steroid metabolism</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="238" mass="25460" checksum="C36B395AA2D8317C" modified="2013-07-24" version="1">MANILVTGGSRGIGAAIAKKFAEQGDTVYITYCKSKEAAQALEQQYGIHPIFADFREERGAFKSGKAIAEQIGTIDTLVNNAAISVVDVFQCVSEEDCTALYRVNLFGTVDMTRAILPTMLQQHAGNIINITSVWGQTGASCEVDYSVTKGGIIAFTKALAKEVAPSGIRVNAVSPGVIDTEMNAHLSEEDRKELAEETPLQRIGKPEEVADAVLFLASERAAFITGQVLPVNGGFFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5VLP0</accession>
    <name>R5VLP0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00360</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000237">
        <property type="protein sequence ID" value="CCZ84461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00360"/>
    <dbReference type="EnsemblBacteria" id="CCZ84461">
        <property type="protein sequence ID" value="CCZ84461"/>
        <property type="gene ID" value="BN566_00360"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006938">
        <property type="entry name" value="DUF624"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04854">
        <property type="entry name" value="DUF624"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="158"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="209"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="233"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="326" mass="36471" checksum="4FA632EE42BE29F7" modified="2013-07-24" version="1">MGLFGKGFTHAGSGISKTAPKKKGFFRYWELFREKFWKLIELNIFYTLFFIPLIAAAVGLILMLSGSLSVNVSLILVIGGTLLFMILFGPATAGMMKIMRNFFVEKPTFLVHDFLKTFRSEFGHSAIFGFLDCLLVCCVAASFYVYPKLAEQTGSKIYYVMLIVTLSISLVAILMNFYLFLMIVSTNLSMKNIFRNSFYLGIVCMKQNLFSLGILAVVVGGILLLALKTNMMVLMIILTLLPFIPGTWLALMVTVNSYPSIQKYIINPYYEKRGEVNPEIELTRPASADEAVFSDMGGMEKPVDLKVQPVKTSKNPAKEHKGKIIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5VM28</accession>
    <name>R5VM28_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF1540 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00447</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000247">
        <property type="protein sequence ID" value="CCZ84586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00447"/>
    <dbReference type="EnsemblBacteria" id="CCZ84586">
        <property type="protein sequence ID" value="CCZ84586"/>
        <property type="gene ID" value="BN566_00447"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011437">
        <property type="entry name" value="DUF1540"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07561">
        <property type="entry name" value="DUF1540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1540" evidence="1">
        <location>
            <begin position="9"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="58" mass="6597" checksum="F131F60B30766A9F" modified="2013-07-24" version="1">MEMKKNDHIHCSVTQCKHNMGTENYCCLNSITIGTHEPNPTVPECTDCFSFVKRSCCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5VMD1</accession>
    <name>R5VMD1_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Multidrug export protein MepA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_00559</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family. MepA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000273">
        <property type="protein sequence ID" value="CCZ84706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84706">
        <property type="protein sequence ID" value="CCZ84706"/>
        <property type="gene ID" value="BN566_00559"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13143">
        <property type="entry name" value="MATE_MepA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045070">
        <property type="entry name" value="MATE_MepA-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006603">
        <property type="entry name" value="DinF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0046">Antibiotic resistance</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="56"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="90"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="135"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="166"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="192"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="236"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="280"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="321"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="351"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="391"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="418"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ84706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="446" mass="48551" checksum="96F8C0DDF21ECAD8" modified="2013-07-24" version="1">MSKKIQLSDHFTYGRLLRFVMPSILMIFCTSVYTIVDGFFVSNYVGKTAFAALNLVWPVLMAVSTIGFLIGTGGCALVSKTLGEGNRERANQYFSMLITVTFIGTVILSAIGFLLTPQIVTLLGAKGAEMQANSILYGRILFVGIPFMVLQQAFLSFYVAAEKPSLSLIVSIVSGVINMILDYVLIVPLDMGLAGAALATVLSQVIGGIFPVIYFLRKNSSLLRLQFPFPMHWKALWIACANGSSEMVSNLSVSLVSMLYNYQLMRLVGEDGISAYGALMYVSVVFNAVFMGYSIGSAPIVSYHYGAKNHAELKNLFQKGLWLISVSSVVITALAIGFAKQQAMIFTSHDADLMALTIQACQIYSIAYLFKGINTWASSFFTALNNGLVSALISFLRTFLFEIAAILILPALFGVLGIWFSVVAAELAALVVSIAFLAANRKKYQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5VQB8</accession>
    <name>R5VQB8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00582</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000007">
        <property type="protein sequence ID" value="CCZ82777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00582"/>
    <dbReference type="EnsemblBacteria" id="CCZ82777">
        <property type="protein sequence ID" value="CCZ82777"/>
        <property type="gene ID" value="BN566_00582"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ82777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="155" mass="17354" checksum="810A5C3FD1E1621E" modified="2013-07-24" version="1">MLYTPLTKKALKLAYAAHAGQVDKAGMPYIFHPYEVALQVQAEEEVCAALLHDVVEDTDWTMEDLRAAGFPETVLEAVQLLTHDPAVPYLDYVRALCGNPIAVAVKRADLHHNSERTRFDADSPDIEKRLQKYAAARAVLDEAEKEKSSEFKRNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5VQN0</accession>
    <name>R5VQN0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aspartate semialdehyde dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00684</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000021">
        <property type="protein sequence ID" value="CCZ82882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00684"/>
    <dbReference type="EnsemblBacteria" id="CCZ82882">
        <property type="protein sequence ID" value="CCZ82882"/>
        <property type="gene ID" value="BN566_00684"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016620">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000534">
        <property type="entry name" value="Semialdehyde_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012280">
        <property type="entry name" value="Semialdhyde_DH_dimer_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01118">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02774">
        <property type="entry name" value="Semialdhyde_dhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00859">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Semialdhyde_dh" evidence="1">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="292" mass="32302" checksum="635A431155847497" modified="2013-07-24" version="1">MLDAQADIEKIAGMVDFVFCAVDMKKDEIRALEEAYAKAECPVVSNNSAHRFTPDVPMVIPEVNPEHLDIIASQRKRLGTKKGFIAVKSNCSIQSYVPALSPLRKYGIKQVLACTYQAISGAGKTFQTWPEMVDNVIPFIGGEEEKSEKEPLKVWGKVEGDQIVSTETPLITTQCLRVPVSDGHTAAVFVSFDQKPSKEEILNAWKEFAGVPQELELPHAPKQFIHYFEEDNRPQAKVDRNLEGGMAVSVGRLREDTLFDYKFVCLSHNTLRGAAGGAVLLAELLAAKGYFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5VQZ0</accession>
    <name>R5VQZ0_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00767</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000032">
        <property type="protein sequence ID" value="CCZ82967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82967">
        <property type="protein sequence ID" value="CCZ82967"/>
        <property type="gene ID" value="BN566_00767"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="SSF56436"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="104"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="3">
        <location>
            <begin position="185"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ82967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="322" mass="35562" checksum="1715BBA8EF7A7874" modified="2013-07-24" version="1">MYCWNCAHVVNDGEKKCPSCGASRKIKKGTKIHYCPNCGKSTLGKEKCKYCGFDVILKKVVQEEEAVDVKETAPTESAPVKTSDDLSVDKQVPLQVQKSRSNKAVIPALFIAAIVLVGAGSFVLLNQSNQDSSNHEGMQVSEPDSGNDEESGIQETETEEVVTTEVVVTTTTEATTTIVTTTTIPKNHHYELVQGAVSWEEANSRAIAAGGRLAEVHSEDDWNKLIAAVGNTDVYFIWLGAKRTSDYSPMYWNSGDEVDSNWSHWYGNEPSYVDATNPSITEPYLLLWWLKDSGWSLNDANASAVLSTYKDYRIGYAIEYDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>R5VRA8</accession>
    <name>R5VRA8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">FtsK/SpoIIIE family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_00875</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FtsK/SpoIIIE/SftA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000052">
        <property type="protein sequence ID" value="CCZ83087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00875"/>
    <dbReference type="EnsemblBacteria" id="CCZ83087">
        <property type="protein sequence ID" value="CCZ83087"/>
        <property type="gene ID" value="BN566_00875"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041027">
        <property type="entry name" value="FtsK_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002543">
        <property type="entry name" value="FtsK_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018541">
        <property type="entry name" value="Ftsk_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17854">
        <property type="entry name" value="FtsK_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09397">
        <property type="entry name" value="FtsK_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01580">
        <property type="entry name" value="FtsK_SpoIIIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00843">
        <property type="entry name" value="Ftsk_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50901">
        <property type="entry name" value="FTSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="85"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="172"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="196"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="FtsK" evidence="6">
        <location>
            <begin position="510"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="527"/>
            <end position="534"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ83087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="855" mass="94002" checksum="6B6263686217AE30" modified="2013-07-24" version="1">MGRTAKSKPKKKATPKNSDHQNTQGFNAAEIKGAADADTKKEEKLTEKQKTDLKFKSVLLMVSAALVLLLVFIPGSSGWRWMHNGLLGLFGVSVLTWPIVLVLAGLFLQRQIQGQKPKEHRPKDVSTYFLYGLGIAVLVSSTVQVWFGETLRTKNPIDALIAFYHAGQDNPLFNGGAISVLAYILSALLGSPGDKICVLFVLFLVVMWLMGWDMLDLKDWVCRAGGFLRNLLFPTKDALPAEEDVPDLEEPKRIGFFSNLFHKAEEQEQEAEAVQASEAQNTQELDAGEIIAATDGLAETPIPPQEPPLQEPFQMQYDAVEPTLDVEPPEPEEPIFEPESEQMPRQLDLFQEQGMEAVVPENAMHSEQEEPAYQIPSLSLLKRSNNTGKSPEIQQELERNKRMLEETLKDFNVGVSMKDVQRGPSVTRYEVQPNAGVKVSKITGLSDDIALNLSAQSIRIEAPVPGKSVVGIEVPNRKKDVVKLRDILESDAFQTAKSQLAFAVGKDIAGNAIIGDIAKMPHLIIAGATGSGKSVCTNSIIMSILFHATPEQVRLILIDPKIVEFRVYEDIPHLLIPVVTDPKKAAGALNWAVQEMEHRYQTFADNGVRDLADYNKKIQSQPDGQTLPQIVIAIDELADLMMTASKEVEDAICRLAQKARAAGMHLIIATQRPTTDIITGLIKANIPSRIALSVMSQVDSRTILDTGGAEKLLGHGDMLYLPNGMPKPIRVQGCFVSTEEIEKTVAFVKQQAQAAYDDSALVGVEQNIPVLKGETASPDGNADPRLEEAIQAVVELGQASTSSLQRYLNVGYARAARMIDELEKMGIVGGYNGAKPRKVLMTKQQYEERKMRNLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>R5VSD1</accession>
    <name>R5VSD1_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Arginine repressor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">argR</name>
        <name evidence="10" type="ORF">BN566_01211</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Regulates arginine biosynthesis genes.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis; L-arginine biosynthesis [regulation].</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the ArgR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000105">
        <property type="protein sequence ID" value="CCZ83437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01211"/>
    <dbReference type="EnsemblBacteria" id="CCZ83437">
        <property type="protein sequence ID" value="CCZ83437"/>
        <property type="gene ID" value="BN566_01211"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00068"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034618">
        <property type="term" value="F:arginine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051259">
        <property type="term" value="P:protein complex oligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00173">
        <property type="entry name" value="Arg_repressor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001669">
        <property type="entry name" value="Arg_repress"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020899">
        <property type="entry name" value="Arg_repress_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036251">
        <property type="entry name" value="Arg_repress_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020900">
        <property type="entry name" value="Arg_repress_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34471">
        <property type="entry name" value="PTHR34471"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01316">
        <property type="entry name" value="Arg_repressor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02863">
        <property type="entry name" value="Arg_repressor_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01467">
        <property type="entry name" value="ARGREPRESSOR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55252">
        <property type="entry name" value="SSF55252"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01529">
        <property type="entry name" value="argR_whole"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="3 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0678">Repressor</keyword>
    <keyword evidence="6 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Arg_repressor" evidence="8">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="Arg_repressor_C" evidence="9">
        <location>
            <begin position="75"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ83437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="153" mass="17179" checksum="54AAD9285AFA031E" modified="2013-07-24" version="1">MGKKERHAAIMELIQQKELETQEDLIQALLEQGISVTQATISRDIRELQLVKQHLENGVCCYRLPEQTPISDHLLRDAILRTDYAGQIAVLHCRSGTAQAICAAVDAKQDHRIVGTIAGDDTIFILLRSEEQAAFFAKQYAVWMQTEEGETIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5VTJ4</accession>
    <name>R5VTJ4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01582</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000166">
        <property type="protein sequence ID" value="CCZ83847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83847">
        <property type="protein sequence ID" value="CCZ83847"/>
        <property type="gene ID" value="BN566_01582"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025470">
        <property type="entry name" value="DUF4321"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14209">
        <property type="entry name" value="DUF4321"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="89" mass="9631" checksum="3BEDD8692D07AF05" modified="2013-07-24" version="1">MEKFKKILLYILVYVVALAVGGVLAAIGEKITALGFLSTAMDWGFDTIHLNLKVINLALALQFHISVGQIIMIVVCIFTAPKIVGKLTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5VTT0</accession>
    <name>R5VTT0_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 11">Anthranilate synthase component 1</fullName>
            <ecNumber evidence="5 11">4.1.3.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">trpE</name>
        <name evidence="14" type="ORF">BN566_01656</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentrations of ammonia.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>chorismate + L-glutamine = anthranilate + H(+) + L-glutamate + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:21732"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="4.1.3.27"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 11">Heterotetramer consisting of two non-identical subunits: a beta subunit (TrpG) and a large alpha subunit (TrpE).</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the anthranilate synthase component I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.27" evidence="5 11"/>
    <dbReference type="EMBL" id="CBAR010000173">
        <property type="protein sequence ID" value="CCZ83927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01656"/>
    <dbReference type="EnsemblBacteria" id="CCZ83927">
        <property type="protein sequence ID" value="CCZ83927"/>
        <property type="gene ID" value="BN566_01656"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00040"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004049">
        <property type="term" value="F:anthranilate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005801">
        <property type="entry name" value="ADC_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019999">
        <property type="entry name" value="Anth_synth_I-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006805">
        <property type="entry name" value="Anth_synth_I_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005256">
        <property type="entry name" value="Anth_synth_I_PabB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015890">
        <property type="entry name" value="Chorismate_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11236">
        <property type="entry name" value="PTHR11236"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04715">
        <property type="entry name" value="Anth_synt_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00425">
        <property type="entry name" value="Chorismate_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00095">
        <property type="entry name" value="ANTSNTHASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56322">
        <property type="entry name" value="SSF56322"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00564">
        <property type="entry name" value="trpE_most"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9 11" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="10 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Anth_synt_I_N" evidence="13">
        <location>
            <begin position="28"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Chorismate_bind" evidence="12">
        <location>
            <begin position="222"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00020653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CCZ83927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="488" mass="54546" checksum="5FDB43C53D358674" modified="2013-07-24" version="1">MLTPSLEQVKQLAKQYNTIPVFYDFSADNQTPINLYRAMSEGAKNAFIFESVNNGEQWGRYSFVGANPKQEIQMHSTTACILESNQKKTFMVEHPILFLKERMAQYSAPVLPDAPNFTGGLIGYFGYDTVRYMEKTLVNVPEDDIHMPDCDLFLYEELVAYDHLKHNGIVIQNLHTDGNLDAQYAAALWRAEELAQKIRTYNPTPKPTLKTGTAIVHSNTSKDAYMDIVRQAKEHIVNGDIFQIVLSQRFEIQNPPDSFDVYRKLRVSNPSPYLYYFHTPTYDVAGASPEMLASVKNGTIHNRPIAGTKPRGKTREEDMQNETDLLADPKERAEHTMLVDLGRNDVGKVSEFGSVSVTRYMVTERASKVMHLVSDVEGTLRKDLTALDALMAILPAGTLSGAPKVRAMELIDQFEPKKRGLYGGTVGYLGFNGNMDTCIAIRTVLFTNQKAYVQAGAGIVADSVPENEYYETVNKALAVINAIKEAAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5VUJ0</accession>
    <name>R5VUJ0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">O-acetylhomoserine sulfhydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_01917</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the trans-sulfuration enzymes family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01917"/>
    <dbReference type="EnsemblBacteria" id="CCZ84192">
        <property type="protein sequence ID" value="CCZ84192"/>
        <property type="gene ID" value="BN566_01917"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00614">
        <property type="entry name" value="CGS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006235">
        <property type="entry name" value="OAc-hSer/O-AcSer_sulfhydrylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001434">
        <property type="entry name" value="CGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01326">
        <property type="entry name" value="OAH_OAS_sulfhy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00868">
        <property type="entry name" value="CYS_MET_METAB_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="3">
        <location>
            <position position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001434-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ84192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="427" mass="46384" checksum="E200086E3C260884" modified="2013-07-24" version="1">MKQYHFETLQVHAGQEQPDPTTDARAVPIYATTSYVFKDSAQAAGRFALTEPGNIYTRLMNPTNSVFEERMAKLEGGVGALATASGSAAITYAVQNIATVGDHIVSASNLYGGTHNLFSNTLKEQGLETTFVNSKDPADFEAAIQENTKLIYAETLGNPNSDIIDLEALAAVAHKHGIPLIVDSTFATPYLLRPLEHGADVVVHSATKFIGGHGTVMGGIIIDGGKFDWTQNDKFPGLSKPNPSYHGVVFTEACGNAAYIMKIRTTLMRDQGAVLSPFNSFLLLQGLETLSLRVERHVENALKVVDFLKNHPQVEKVYHPSLSTGKEKELYDKYFPNGAGSIFTFTIKGDAEKAKKFTESLELFSLLANVADVKSLVIHPASTTHSQLDEEELLACGIHPNTIRLSIGTEHIDDILADLQHGFDAVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5VV13</accession>
    <name>R5VV13_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ParB-like partition proteins</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00038</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ParB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000219">
        <property type="protein sequence ID" value="CCZ84357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00038"/>
    <dbReference type="EnsemblBacteria" id="CCZ84357">
        <property type="protein sequence ID" value="CCZ84357"/>
        <property type="gene ID" value="BN566_00038"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041468">
        <property type="entry name" value="HTH_ParB/Spo0J"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004437">
        <property type="entry name" value="ParB/RepB/Spo0J"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003115">
        <property type="entry name" value="ParB/Sulfiredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036086">
        <property type="entry name" value="ParB/Sulfiredoxin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17762">
        <property type="entry name" value="HTH_ParB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02195">
        <property type="entry name" value="ParBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00470">
        <property type="entry name" value="ParB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110849">
        <property type="entry name" value="SSF110849"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00180">
        <property type="entry name" value="parB_part"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ParB" evidence="3">
        <location>
            <begin position="18"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="285" mass="32471" checksum="02779BCA3DD18624" modified="2013-07-24" version="1">MMTGFLTSTKERRQRPILAVRISDILCQPDNQQDPKTLQALANRITQTGILQPFPVRKNADQRYELVSGSEQFQAAKLAGLQIVPCILSDNHSRNAEILALVEQIQQQNLSFFEEASAIERLISHYGMTQEDAAAKLGKAQSTIANKLRLLRLTETERDIILQYNLTERHARALLRLRAPEERLQILDKVIKQNLNVEKTEAAVEEKIGQHPSREPYRKRNTVFQNVRAFAHTIHQAVETMQAAGISADAQKLQSDTHIEYRIRIPVPQQNVHSEQPPRNRLPLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5VV57</accession>
    <name>R5VV57_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Predicted membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_00304</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000224">
        <property type="protein sequence ID" value="CCZ84397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00304"/>
    <dbReference type="EnsemblBacteria" id="CCZ84397">
        <property type="protein sequence ID" value="CCZ84397"/>
        <property type="gene ID" value="BN566_00304"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010540">
        <property type="entry name" value="CmpB_TMEM229"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06541">
        <property type="entry name" value="ABC_trans_CmpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="102"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ84397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="132" mass="15090" checksum="1D8F2B2F5799B1FF" modified="2013-07-24" version="1">MTAPQKIHAYEYGIMFLLGCFIYSLLEIAARGYTHWTMTLLGGIVGMLLYRLHGTAPRHTLLLQALSGAFIITALEMVVGVLDNLVLQWHVWSYREMPWNVYGQICLPFSVLWFLVCIPALGICEVVRKRFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVB1</accession>
    <name>R5VVB1_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">zinc_ribbon_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00349</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000229">
        <property type="protein sequence ID" value="CCZ84442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84442">
        <property type="protein sequence ID" value="CCZ84442"/>
        <property type="gene ID" value="BN566_00349"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026870">
        <property type="entry name" value="Zinc_ribbon_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13240">
        <property type="entry name" value="zinc_ribbon_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="zinc_ribbon_2" evidence="2">
        <location>
            <begin position="3"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="268" mass="29266" checksum="C83B5DDA9A74569F" modified="2013-07-24" version="1">MTCKKCGTSLEPNDRFCYACGAPVEPQAPTPVIPSIPSPPPAPTPAPAPVVTPIPTPAATETNAATAAQPTATATVPPVPENPLQATLAQSAVTVKPSKRNMTLGFLSIGVGCLIIIGILCYLFSNNGYRSAVKEMWYGVERQDASGAAANMFPSEASSQLDLTEQTMETYLSTFQEHAARYGDNYHISIQFDDVEELDTEQLEQFNIWFQNLSLEKEVNKTYAVDYTRSISGKNGKESEHRFCYIYHYDGNWYVLDTALLGLQDPLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVC4</accession>
    <name>R5VVC4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Putative agmatine deiminase</fullName>
            <ecNumber evidence="2">3.5.3.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Agmatine iminohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">aguA</name>
        <name evidence="3" type="ORF">BN566_00351</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>agmatine + H2O = N-carbamoylputrescine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:18037"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:58145"/>
            <dbReference type="ChEBI" id="CHEBI:58318"/>
            <dbReference type="EC" id="3.5.3.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the agmatine deiminase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.3.12" evidence="2"/>
    <dbReference type="EMBL" id="CBAR010000231">
        <property type="protein sequence ID" value="CCZ84452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00351"/>
    <dbReference type="EnsemblBacteria" id="CCZ84452">
        <property type="protein sequence ID" value="CCZ84452"/>
        <property type="gene ID" value="BN566_00351"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047632">
        <property type="term" value="F:agmatine deiminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004668">
        <property type="term" value="F:protein-arginine deiminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009446">
        <property type="term" value="P:putrescine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01841">
        <property type="entry name" value="Agmatine_deimin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017754">
        <property type="entry name" value="Agmatine_deiminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007466">
        <property type="entry name" value="Peptidyl-Arg-deiminase_porph"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31377">
        <property type="entry name" value="PTHR31377"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04371">
        <property type="entry name" value="PAD_porph"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03380">
        <property type="entry name" value="agmatine_aguA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Amidino-cysteine intermediate" evidence="2">
        <location>
            <position position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="364" mass="40210" checksum="EA5BA344A7C12F0C" modified="2013-07-24" version="1">MHILKHLPKQDGCRMPAEFASHLGCIMIWPERTDSWRNGGYAARAAFTKIATAIAASEQVTMLVSNQQYETARAMLPPEVRLVECTTDDAWARDVAPTFVLRPDGTRCGIDWGFNAWGGLVDGLYFPWNNDNHVARKVCDLFDCDCYDLRHFILEGGSIHSDGEGTILTTEACLLSAGRNPELSKEAITQMLCDCLGAEKVIWLPCGIYQDETNEHVDNICAFTEPGKVVLAWTDNPNDPQYAMSNACLQVLEQETDAKGRKIQVQKLPIPSEPICMTEEECSGLDLWDGEPTRTPGERLAASYVNFYISNGAVIVPQFNDPMDAVAVSVLQDCFPTRTVVPIAAREILIGGGNIHCITQQIPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVK2</accession>
    <name>R5VVK2_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">50S ribosomal protein L4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rplD</name>
        <name evidence="8" type="ORF">BN566_00058</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Forms part of the polypeptide exit tunnel.</text>
    </comment>
    <comment type="function">
        <text evidence="6">One of the primary rRNA binding proteins, this protein initially binds near the 5'-end of the 23S rRNA. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 6">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uL4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000241">
        <property type="protein sequence ID" value="CCZ84512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00058"/>
    <dbReference type="EnsemblBacteria" id="CCZ84512">
        <property type="protein sequence ID" value="CCZ84512"/>
        <property type="gene ID" value="BN566_00058"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01328_B">
        <property type="entry name" value="Ribosomal_L4_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002136">
        <property type="entry name" value="Ribosomal_L4/L1e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023574">
        <property type="entry name" value="Ribosomal_L4_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013005">
        <property type="entry name" value="Ribosomal_uL4/L1e"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10746">
        <property type="entry name" value="PTHR10746"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00573">
        <property type="entry name" value="Ribosomal_L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52166">
        <property type="entry name" value="SSF52166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03953">
        <property type="entry name" value="rplD_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0699">rRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="48"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ84512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="208" mass="22726" checksum="6311144CDB51BA32" modified="2013-07-24" version="1">MAKVSVFDMTGQQVSEIELADAVFGITPNESAMHAMVVNYLANQRQGTQSTLTRSEVRGGGRKPWRQKGTGHARQGSIRAPQWTHGGIALGPKPRDYRYALNKKLRKLAMKSALSTKVLDSNLIVVDSLTTDSYKTKTVVAMLKALNVDGKAMLVTVDKDEKLVKSAANIPGVKTAACNTLNVYDILHHDKFIVSKDAVAKIEEVYAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVY2</accession>
    <name>R5VVY2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00545</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000267">
        <property type="protein sequence ID" value="CCZ84692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84692">
        <property type="protein sequence ID" value="CCZ84692"/>
        <property type="gene ID" value="BN566_00545"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="119" mass="13029" checksum="5223BD41906CCE69" modified="2013-07-24" version="1">MRHCQYCDALNPDDALQCGNCGKSLPPAGKNIVSSSDKIPENAGLKLQVLARIIFAVILVCGEILGYLNIKEEEIAIGVAYVLISPLLAYLNMIVLHAFGELCQNVSGARYYLYQLNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>R5UWU8</accession>
    <name>R5UWU8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Phenylalanine--tRNA ligase alpha subunit</fullName>
            <ecNumber evidence="13">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Phenylalanyl-tRNA synthetase alpha subunit</fullName>
            <shortName evidence="13">PheRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">pheS</name>
        <name evidence="15" type="ORF">BN566_00603</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + H(+) + L-phenylalanyl-tRNA(Phe)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="13">Binds 2 magnesium ions per tetramer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 13">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="13"/>
    <dbReference type="EMBL" id="CBAR010000008">
        <property type="protein sequence ID" value="CCZ82798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00603"/>
    <dbReference type="EnsemblBacteria" id="CCZ82798">
        <property type="protein sequence ID" value="CCZ82798"/>
        <property type="gene ID" value="BN566_00603"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00281">
        <property type="entry name" value="Phe_tRNA_synth_alpha1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004529">
        <property type="entry name" value="Phe-tRNA-synth_IIc_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004530">
        <property type="entry name" value="Phe-tRNA-synth_IIc_mito"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004188">
        <property type="entry name" value="Phe-tRNA_ligase_II_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022911">
        <property type="entry name" value="Phe_tRNA_ligase_alpha1_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002319">
        <property type="entry name" value="Phenylalanyl-tRNA_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538:SF41">
        <property type="entry name" value="PTHR11538:SF41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02912">
        <property type="entry name" value="Phe_tRNA-synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01409">
        <property type="entry name" value="tRNA-synt_2d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="SSF46589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00468">
        <property type="entry name" value="pheS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 13" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="9 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 13 15" id="KW-0436">Ligase</keyword>
    <keyword evidence="10 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11 13" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II" evidence="14">
        <location>
            <begin position="113"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CCZ82798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="339" mass="37988" checksum="356BC8A2D19889DA" modified="2013-07-24" version="1">MKEQLEAIRQEAEAALQTCTDAKQLDAIRVQYLGKKGALTAILKQMGKLSAEERPVMGQLANAVRSDIESAITKQQAAIAEAALEQKLQSETLDITLPGKQKKIGGLHPLTIVENEIKEIFLGMGFSVADGPEVEYDYYNFEALNLPPDHPARDTQDTFYITDNILLRTQTSSVQVHVMEQQKPPIRVISPGRVFRSDAIDATHSPLFHQVEGLVVDKGITMADLKGTLELLMQRLYGDDCKIRLRPHHFPFTEPSAEVDVMCYNCYGKGCRLCKGEGYIELLGAGMVHPKVLEGCGIDSNVYSGFAFGLGLERIVMRRYNIQDMRLLFENDYRFLEQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXM9</accession>
    <name>R5UXM9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00635</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000012">
        <property type="protein sequence ID" value="CCZ82830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00635"/>
    <dbReference type="EnsemblBacteria" id="CCZ82830">
        <property type="protein sequence ID" value="CCZ82830"/>
        <property type="gene ID" value="BN566_00635"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ82830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="135" mass="14734" checksum="05D77CC411FFC9C7" modified="2013-07-24" version="1">MIKLITGKKGSGKTKILIDQINNAVKDTNGCLVCVEKGETLRRSISYKVRWCDAEQFAIDSFAAFYGFVAGMLAGNYDIKEIFVDGIFRIAGADFDEFGLMLGKLDKLTGEDVTVAFTVSADNAELPSSVTKFLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXS5</accession>
    <name>R5UXS5_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">4-hydroxy-tetrahydrodipicolinate reductase</fullName>
            <shortName evidence="9">HTPA reductase</shortName>
            <ecNumber evidence="9">1.17.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">dapB</name>
        <name evidence="12" type="ORF">BN566_00682</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the conversion of 4-hydroxy-tetrahydrodipicolinate (HTPA) to tetrahydrodipicolinate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(S)-2,3,4,5-tetrahydrodipicolinate + H2O + NAD(+) = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:35323"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:67139"/>
            <dbReference type="EC" id="1.17.1.8"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(S)-2,3,4,5-tetrahydrodipicolinate + H2O + NADP(+) = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:35331"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:67139"/>
            <dbReference type="EC" id="1.17.1.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="9">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 4/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the DapB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Was originally thought to be a dihydrodipicolinate reductase (DHDPR), catalyzing the conversion of dihydrodipicolinate to tetrahydrodipicolinate. However, it was shown in E.coli that the substrate of the enzymatic reaction is not dihydrodipicolinate (DHDP) but in fact (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid (HTPA), the product released by the DapA-catalyzed reaction.</text>
    </comment>
    <dbReference type="EC" id="1.17.1.8" evidence="9"/>
    <dbReference type="EMBL" id="CBAR010000021">
        <property type="protein sequence ID" value="CCZ82880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00682"/>
    <dbReference type="EnsemblBacteria" id="CCZ82880">
        <property type="protein sequence ID" value="CCZ82880"/>
        <property type="gene ID" value="BN566_00682"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00018"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008839">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate reductase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016726">
        <property type="term" value="F:oxidoreductase activity, acting on CH or CH2 groups, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00102">
        <property type="entry name" value="DapB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022663">
        <property type="entry name" value="DapB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000846">
        <property type="entry name" value="DapB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022664">
        <property type="entry name" value="DapB_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023940">
        <property type="entry name" value="DHDPR_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20836">
        <property type="entry name" value="PTHR20836"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05173">
        <property type="entry name" value="DapB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01113">
        <property type="entry name" value="DapB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000161">
        <property type="entry name" value="DHPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00036">
        <property type="entry name" value="dapB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01298">
        <property type="entry name" value="DAPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword evidence="8 9" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="7 9" id="KW-0520">NAD</keyword>
    <keyword evidence="4 9" id="KW-0521">NADP</keyword>
    <keyword evidence="6 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DapB_N" evidence="10">
        <location>
            <begin position="2"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="DapB_C" evidence="11">
        <location>
            <begin position="115"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="(S)-2,3,4,5-tetrahydrodipicolinate binding" evidence="9">
        <location>
            <begin position="152"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="9">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="9">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="8"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="85"/>
            <end position="87"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="109"/>
            <end position="112"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>(S)-2,3,4,5-tetrahydrodipicolinate</name>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CCZ82880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="252" mass="27590" checksum="EDF7E19D2D196821" modified="2013-07-24" version="1">MTKIVLCGANGKMGHTLAGCIAKREDCTVIAGIDSYTKQYDSFPIVETAEQLPEQPDVIIDFSNPSSLDMLLNYAFTHHVALVLATTGYSEAQIAQIHTAAEQIPVFFTFNMSLGINLLVELAKRATAILGDQFDIEIVEKHHNQKIDAPSGTAIMLANAINEVKDNRYHYVYDRHARRMKREKNEIGMHAIRGGTIVGEHDVIFAGNDEVITLSHSAASKTVFAEGAVNAAVFLSGKPAGLYDMKQLIEQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5UY32</accession>
    <name>R5UY32_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">TSCPD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01019</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000081">
        <property type="protein sequence ID" value="CCZ83238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83238">
        <property type="protein sequence ID" value="CCZ83238"/>
        <property type="gene ID" value="BN566_01019"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023806">
        <property type="entry name" value="CHP03905"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024434">
        <property type="entry name" value="TSCPD_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12637">
        <property type="entry name" value="TSCPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03905">
        <property type="entry name" value="TIGR03905_4_Cys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TSCPD" evidence="1">
        <location>
            <begin position="5"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="84" mass="9090" checksum="92238307FE845758" modified="2013-07-24" version="1">MQYQYRPSGVCSRQITIDVENGVVQNVSFVGGCNGNLQGIGALVKGMKIEDVITRLEHIRCNGKPTSCPDQLAQALKKIQAETA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYF9</accession>
    <name>R5UYF9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Chromosome segregation ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00114</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000046">
        <property type="protein sequence ID" value="CCZ83060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00114"/>
    <dbReference type="EnsemblBacteria" id="CCZ83060">
        <property type="protein sequence ID" value="CCZ83060"/>
        <property type="gene ID" value="BN566_00114"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025669">
        <property type="entry name" value="AAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13614">
        <property type="entry name" value="AAA_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_31" evidence="2">
        <location>
            <begin position="4"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="250"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="271" mass="30098" checksum="69E1B358FEC8E6FA" modified="2013-07-24" version="1">MGLVYAIANQKGGVGKTTTVVNLAAYFGARGKRVLCIDIDPQGNTTTGLGIKKRSLTASSYHVLNGSAKISDVIQQTEYENVSIVPARDELAGCELELSQMEYRATRLRMQLLTCKADYDYIFIDCPPALGLLTLNGLAAADEIIVPMTAEFYALEGLSQLMTTMKAVRANLNPSLKIAGILFVMFDPRLNVSRQVEEEVAKYFPNQIFRTKIPRNVRLSEAPSYGKPVMYYDKNSKGAEAYAELGMELLGETPPPKEKKRFQLFHRKNKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYI4</accession>
    <name>R5UYI4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Beta-N-acetylhexosaminidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01154</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000093">
        <property type="protein sequence ID" value="CCZ83378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01154"/>
    <dbReference type="EnsemblBacteria" id="CCZ83378">
        <property type="protein sequence ID" value="CCZ83378"/>
        <property type="gene ID" value="BN566_01154"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002105">
        <property type="entry name" value="Dockerin_1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001764">
        <property type="entry name" value="Glyco_hydro_3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036962">
        <property type="entry name" value="Glyco_hydro_3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00404">
        <property type="entry name" value="Dockerin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00933">
        <property type="entry name" value="Glyco_hydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004406559" evidence="2">
        <location>
            <begin position="30"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="3">
        <location>
            <begin position="36"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="530" mass="57951" checksum="FA0ACD96C030E23E" modified="2013-07-24" version="1">MKQTHFFAAVVTAGMMCLSTFSVQGYAGTDNSKDVVETVIGDIDQNGVVDPADAIAILKINANENAYSENQIKAADVNADGAITAEDATMVLTFYAKTAAGMKPIWNSNAIFTFSDDNMKCKKNDENYTGMIANADSDVYYYQDGIASKGVYEIDGEKHYFSYETGIMQKESADGYVIEDGTVIYEPTSPLLSSDLFGAYYERATEIMNTMTLEEKVGQLFLIGIAQTNGEEIVRDYHPGGIVMFAYNFSDQTVASEKEQIASYQANSRFPLLISADEEGGTVVRVSKYPAFRDTPFLSPQELYAQGGWDGVYQETIEKGNLLKSLGINLCLAPVADISNNPNDYIYERTFGGSAEDTSQFIEISTKAYRETNTGCTLKHFPGYASNLNTHNGSSVDNREQSQFYGNDLKPFQAGIDAGAPVVMVNHNIVSCFDAENPASISPEVHSVLRKDLHFSGLICTDSLDMGATKNVTDVYLKAVDAGNDLIATCETAGYPIVLQAVHTGRISENRVNESVIRILAYKLYYGLMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYI6</accession>
    <name>R5UYI6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00878</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000053">
        <property type="protein sequence ID" value="CCZ83090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83090">
        <property type="protein sequence ID" value="CCZ83090"/>
        <property type="gene ID" value="BN566_00878"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="304" mass="34902" checksum="FB5E712DA83A8A25" modified="2013-07-24" version="1">MKLHTLSEFSKTVMRQTEQSVSYFFWSWLGGCILWMLCRIAFCYAVQISFTFLGLLPALEATWYGLRLMFAVGGFLWLTSLLYRCLYACAAAASLTKQPAPKKGAVYRMALCLRGLRLLVLLPLPLCLWSLRWCLEQGTTVSDGVWWLWTGMQFLTLAVLFLAGWLWLLPVCLAAPVLQVLFPEHRCWSLVCDALTLMEGRRKEWYRLMLRSLPFWLLPHFWSRLAMMQTVYVGVCWVETQTAQRKRKKGGMSRAANTHMANGAGTALHPRTISQKRKKCIPAAADQTEAYQRALVQRNTHPKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYP7</accession>
    <name>R5UYP7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">Glucose-inhibited division protein A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">mnmG</name>
        <name evidence="11" type="synonym">gidA</name>
        <name evidence="14" type="ORF">BN566_00943</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 11">NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="9 11">Homodimer. Heterotetramer of two MnmE and two MnmG subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the MnmG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000069">
        <property type="protein sequence ID" value="CCZ83160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00943"/>
    <dbReference type="EnsemblBacteria" id="CCZ83160">
        <property type="protein sequence ID" value="CCZ83160"/>
        <property type="gene ID" value="BN566_00943"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00129">
        <property type="entry name" value="MnmG_GidA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004416">
        <property type="entry name" value="MnmG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002218">
        <property type="entry name" value="MnmG-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020595">
        <property type="entry name" value="MnmG-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026904">
        <property type="entry name" value="MnmG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044920">
        <property type="entry name" value="MnmG_C_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040131">
        <property type="entry name" value="MnmG_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11806">
        <property type="entry name" value="PTHR11806"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11806:SF0">
        <property type="entry name" value="PTHR11806:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01134">
        <property type="entry name" value="GIDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13932">
        <property type="entry name" value="GIDA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00136">
        <property type="entry name" value="gidA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01280">
        <property type="entry name" value="GIDA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01281">
        <property type="entry name" value="GIDA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11" id="KW-0274">FAD</keyword>
    <keyword evidence="5 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8 11" id="KW-0520">NAD</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="GIDA" evidence="12">
        <location>
            <begin position="9"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="GIDA_C" evidence="13">
        <location>
            <begin position="401"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="13"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="273"/>
            <end position="287"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="370"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF13932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CCZ83160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="628" mass="69696" checksum="EEF84B3017D6BACD" modified="2013-07-24" version="1">MYEMESYTVAVIGAGHAGVEAALSCARMGCKTVLFTISLDQIANMPCNPSIGGTAKGHLVREIDALGGEMGKAADACFLQSRMLNRGKGPAVHSLRVQADRVRYHNYMKQVCEQTENLYIKQAEVAEILVEDGAVCGVVTTLGAKYAVKAAIVATGTYLNGRIHVGTVSYESGPDSALPSRALGKSLQALGLPLRRFKTGTPCRVHSRSIDFDRMEPQDGDAEIVPFSFATPREGLHNTVRCYITYTNAETHRIIRENLNRSPLFTGQISGIGPRYCPSIETKIVRFAEKERHQLFVEPMGMQTEEYYLQGMSSSLPEDVQLAFLRTIRGLEHVEIMRPAYAIEYDCVDPTALRPTLETKQIHGLYGAGQFNGSSGYEEAAAQGIVAGINAALQAQNKPPMILDRASSYIGTLVDDLVTKGCEDPYRMMTSRSEYRLVLRQDNADFRLMPIGYKVGLLPEARYQEMLKKYELVETEKQRLLKTNVAPSDAFNKFLTEHETAPLSTGCKLADLIRRPQLNYALLKPFDVERPDYPLEIGEQVEIQLKYEGYIEKQMAQIARMRKLEEKSLPETVDYTQIRGLRLEAAEKLNAHRPANIGQASRISGVSPADISVLLVWETALQKGEQPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYW0</accession>
    <name>R5UYW0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01011</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000080">
        <property type="protein sequence ID" value="CCZ83230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01011"/>
    <dbReference type="EnsemblBacteria" id="CCZ83230">
        <property type="protein sequence ID" value="CCZ83230"/>
        <property type="gene ID" value="BN566_01011"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016454">
        <property type="entry name" value="Cysteine_dSase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005572">
        <property type="entry name" value="NifS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotran_5" evidence="2">
        <location>
            <begin position="4"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="376" mass="40873" checksum="54F841FD06AE5EE5" modified="2013-07-24" version="1">MEYYLDHAATTKPCPAAVDALLRCLSTHYGNPSSLHRFGVEAQREVEQARAILAAVLGCTSQEVVFTSGATESNHLAIAGAVQTYGKRKKKLVVSAVEHASVRNTMQFLQDSGYEVVTVAPDATGIYHASDFLDAIDSNTCLLSMMMVNNETGYRLPVQTVFRAAKRKFPELITHCDAVQGFLKVPFQVRSLQADLLSISGHKVHGVKGVGALYQKNGIHLHPTFHGGSQERGLRPGTENVPAIAAFGAAVQTYGSTVRERYQAVTEKKAYLLERLQELPNCKIHSVEDASPYIISFSLRPYRSEILLHFLEKREIFVSSGSACSKGKNSGVPVLFGATDAEADSILRVSLSAETPNTALDALVSSLQEATKTLLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYX3</accession>
    <name>R5UYX3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Chaperone protein DnaJ</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01026</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000081">
        <property type="protein sequence ID" value="CCZ83245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01026"/>
    <dbReference type="EnsemblBacteria" id="CCZ83245">
        <property type="protein sequence ID" value="CCZ83245"/>
        <property type="gene ID" value="BN566_01026"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="SSF46565"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="3"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="204" mass="23392" checksum="C6ED3B192AEBF3DE" modified="2013-07-24" version="1">MSNPYEILGVGEQASDEEVKKAYRKLSRKYHPDANVNSPNAAEYEEKFKQVQQAYQQIMDERHNPNGSFSNFHAESSGGQDEDSIHLQAALNFIQNYRFMEAVRVLNEIKNRDAQWYYISAIANNGAHNYAIALEHAKMAAKLEPDRVEYQRYVEQLQGHNTSYQRMQHTYTPSFDSSCPSCLECCFYNLLCNLFCGCCSPCGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZ89</accession>
    <name>R5UZ89_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01384</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000147">
        <property type="protein sequence ID" value="CCZ83648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83648">
        <property type="protein sequence ID" value="CCZ83648"/>
        <property type="gene ID" value="BN566_01384"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019650">
        <property type="entry name" value="DUF2513"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10711">
        <property type="entry name" value="DUF2513"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="130" mass="14681" checksum="EB04504757CFD9A2" modified="2013-07-24" version="1">MKLNYDCLRSLLLKLEEFENIDENLNYQSMTLDDMEKALPDFPKNVIAYTTLKAEQGGLINAQIMNADNSIYMCIYSSLTYDGHQFLDNVRSNSIWSRTKSIAKKLGCTSLNSLMSIAGKIALDVIQSQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZA2</accession>
    <name>R5UZA2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01141</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000093">
        <property type="protein sequence ID" value="CCZ83365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83365">
        <property type="protein sequence ID" value="CCZ83365"/>
        <property type="gene ID" value="BN566_01141"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="142" mass="16104" checksum="26F2B63B94EDDF08" modified="2013-07-24" version="1">MYKHTCQICGMEFESPSARAKYCIYCRDKAQVMRNKAYKEKKQAGEAVAIGSEQICSICEKPYKVTAGSQKYCKECQQKQARSKKISSNAQYAKANYKTLKLYVSAEERDAIKAYAETLGMSVNKLLLTALEEYQKAHKQEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZH3</accession>
    <name>R5UZH3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Sialate O-acetylesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01483</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000161">
        <property type="protein sequence ID" value="CCZ83748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01483"/>
    <dbReference type="EnsemblBacteria" id="CCZ83748">
        <property type="protein sequence ID" value="CCZ83748"/>
        <property type="gene ID" value="BN566_01483"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001681">
        <property type="term" value="F:sialate O-acetylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005181">
        <property type="entry name" value="SASA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039329">
        <property type="entry name" value="SIAE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22901">
        <property type="entry name" value="PTHR22901"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03629">
        <property type="entry name" value="SASA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="2" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SASA" evidence="3">
        <location>
            <begin position="275"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="501" mass="56642" checksum="C5A15F2DC176819F" modified="2013-07-24" version="1">MLQLAAVFSDHMVLQREKNIVVFGIGEVGAIITVSIPERKLYASTTVKEDGSWRLQFPPQPAGTGLSLFAKDSHTEIHFQDVAIGEVWLAGGQSNMEFPVKNAVNGKLELSRCERSRVRYYHVQRSGVMHAAYFQNERKNRWQMPNPTNVAEWSAVAYLAAKELSQKLGVVVGVIGCNYTGTSASCWVPSADLTGHTALHPYWAAYLSACRGKTDAEMAADYAAYLAYQNAWEKRMTACYQENPKIKWNQVLERCGENRYPGPMGSRNPLRPCGLYKTMLSRVMPYTLRGFWYYQGENDDNRPDSYAQLLTVLINRWRKDWGDETMPFLIVQLPMFSYEGEESRTNWAELREAQMQVFRTLRNTGLAVTMDCGEYNNMHPADKRTVAHRLALQALYRVYRAIPAMDAEAPYCKNAYASGGAYILQFSQPVVAKDGEAAVFELAGTDGVYYPAVAEFRDNFVRLTSPNVRIPASMRYAWRNWCDVGVYGKNGIPVASFRMPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZH7</accession>
    <name>R5UZH7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DNA protecting protein DprA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01488</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DprA/Smf family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000161">
        <property type="protein sequence ID" value="CCZ83753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01488"/>
    <dbReference type="EnsemblBacteria" id="CCZ83753">
        <property type="protein sequence ID" value="CCZ83753"/>
        <property type="gene ID" value="BN566_01488"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009294">
        <property type="term" value="P:DNA-mediated transformation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003488">
        <property type="entry name" value="DprA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041614">
        <property type="entry name" value="DprA_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43022">
        <property type="entry name" value="PTHR43022"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02481">
        <property type="entry name" value="DNA_processg_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17782">
        <property type="entry name" value="DprA_WH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00732">
        <property type="entry name" value="dprA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DprA_WH" evidence="3">
        <location>
            <begin position="359"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="333"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="419" mass="46466" checksum="D42AA5D96E4CF69D" modified="2013-07-24" version="1">MEQESIYDWLWLLLVFGAGSRKIWKLLEQYETPQKVRQVLQTETDLPFIHEREQRSIRSITNEQIGKLMQNCAEKRIELVAYDDENYPESLRQIYNPPVVLFYQGQLSVLSDYPALTVVGTRKPSSYSIRTADLLCRCLAKSGMLLVSGFAVGLDSVAHRAALLSGGKTVAVMGCGLDVPYPKPNASAKKYIMRNGLLLSEFLPGTEPSRQNFPMRNRILAGVSGGTLVIQAPIGSGALITAEQAMEQGKPVFCLPPADIFDNQYAGVVKYLREGAIPVFAPEDILQEYDHGCEQRLIEVSMQAVLEQQEQNQLKSSDSSEAAAAVELPPKKFAKPKAQSAPSISEEQKSAPTVDRSVLETLDPLSKSIMELLLEQSPLHIDQMVLMLQEDAEEVAACLTELAIEQLVQRQPGQYYCVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZT4</accession>
    <name>R5UZT4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DJ-1 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00168</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000168">
        <property type="protein sequence ID" value="CCZ83863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00168"/>
    <dbReference type="EnsemblBacteria" id="CCZ83863">
        <property type="protein sequence ID" value="CCZ83863"/>
        <property type="gene ID" value="BN566_00168"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006287">
        <property type="entry name" value="DJ-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002818">
        <property type="entry name" value="DJ-1/PfpI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01965">
        <property type="entry name" value="DJ-1_PfpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01383">
        <property type="entry name" value="not_thiJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DJ-1_PfpI" evidence="1">
        <location>
            <begin position="2"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="180" mass="18806" checksum="1E85CFBD07844324" modified="2013-07-24" version="1">MIYVFLADGFEETEAIAPIDMLRRAKKEVVTVGVTGKVVTSSHGVPVTADITQDEIQLNDQLEMIVLPGGMPGTLNEEASEQVQAAITYCAEQNRYLTAICAAPSILGHRGLLNGKHAVCYPGFESALTGAEVGTEGVVQDGNIITARGAGVAIDFGLALVAALCGAEKSAEIRKGIVYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZV2</accession>
    <name>R5UZV2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Membrane proteins related to metalloendopeptidases</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_01612</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000169">
        <property type="protein sequence ID" value="CCZ83883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01612"/>
    <dbReference type="EnsemblBacteria" id="CCZ83883">
        <property type="protein sequence ID" value="CCZ83883"/>
        <property type="gene ID" value="BN566_01612"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011055">
        <property type="entry name" value="Dup_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016047">
        <property type="entry name" value="Peptidase_M23"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01551">
        <property type="entry name" value="Peptidase_M23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51261">
        <property type="entry name" value="SSF51261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004406597" evidence="3">
        <location>
            <begin position="27"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M23" evidence="4">
        <location>
            <begin position="442"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="238"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="378"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="178"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="238"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="255"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="312"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="557" mass="61079" checksum="46CE6FF8D840016C" modified="2013-07-24" version="1">MMRLKGMKRFLSVVLCAAILTGGVYSGTGVVKSVPVSAMTLAELQSEKQANADKIAKMQKELDDLGSEQQEQAGFQKTLEEKISLQQSNLQIVDEELTRLEDAITDKEQEICDLKNEVAALDAEISNGLEEFKQRLRVMYVQGNDSLASALVGSTDFYDLLSKYDLMSRVAKHDNELVSNLKEKLETCQQKQQSLEEEKNALAEQKASEKQKKEEFSAALEQLQDDYAMSTDESIRSAVDEHHDHEDDASNPDSVVRDAESQQIQAEIRKSQEAARATATTTTTTTTTTEETTTTTTTTEEAFDVVETTAEETTEETTTTTTTEDPWSVEDWETTTTEETTTTTEPEVTTIPYETVETTTYWEETTTTTETTEAIETEPVVEETTTTTTTEEVTTAPETTTTPETTTTIATTAAPYNESQFAWPVPGFYYLSSGYGPRWDSTHGGIDIAGGGISGASVVASRSGTVIYVSNTCTHNYGKDSSCGCGGGYGNYCIIEHDGTYSTVYGHMASLNVSYGQHVNQGDVIGYVGSTGWSTGYHLHFEIRVNGSRVDPTGYLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>R5V019</accession>
    <name>R5V019_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase</fullName>
            <ecNumber evidence="10">2.1.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Cobalamin-independent methionine synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Methionine synthase, vitamin-B12 independent isozyme</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">metE</name>
        <name evidence="13" type="ORF">BN566_01348</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = L-methionine + tetrahydropteroyltri-L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:21196"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58140"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
            <dbReference type="ChEBI" id="CHEBI:58207"/>
            <dbReference type="EC" id="2.1.1.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="10">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the vitamin-B12 independent methionine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.14" evidence="10"/>
    <dbReference type="EMBL" id="CBAR010000132">
        <property type="protein sequence ID" value="CCZ83580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01348"/>
    <dbReference type="EnsemblBacteria" id="CCZ83580">
        <property type="protein sequence ID" value="CCZ83580"/>
        <property type="gene ID" value="BN566_01348"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00082"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003871">
        <property type="term" value="F:5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03311">
        <property type="entry name" value="CIMS_C_terminal_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.210">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00172">
        <property type="entry name" value="Meth_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013215">
        <property type="entry name" value="Cbl-indep_Met_Synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006276">
        <property type="entry name" value="Cobalamin-indep_Met_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002629">
        <property type="entry name" value="Met_Synth_C/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038071">
        <property type="entry name" value="UROD/MetE-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08267">
        <property type="entry name" value="Meth_synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01717">
        <property type="entry name" value="Meth_synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000382">
        <property type="entry name" value="MeTrfase_B12_ind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51726">
        <property type="entry name" value="SSF51726"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01371">
        <property type="entry name" value="met_syn_B12ind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 10" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="4 10 13" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 10 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Meth_synt_1" evidence="12">
        <location>
            <begin position="3"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="Meth_synt_2" evidence="11">
        <location>
            <begin position="427"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="region of interest" description="5-methyltetrahydropteroyltri-L-glutamate binding" evidence="10">
        <location>
            <begin position="15"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="region of interest" description="L-homocysteine binding" evidence="10">
        <location>
            <begin position="432"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="L-methionine binding" evidence="10">
        <location>
            <begin position="432"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="5-methyltetrahydropteroyltri-L-glutamate binding" evidence="10">
        <location>
            <begin position="516"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="696"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>5-methyltetrahydropteroyltri-L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:58207"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="485"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="562"/>
        </location>
        <ligand>
            <name>5-methyltetrahydropteroyltri-L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:58207"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="600"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="606"/>
        </location>
        <ligand>
            <name>5-methyltetrahydropteroyltri-L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:58207"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="643"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="645"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="667"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="728"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CCZ83580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="758" mass="84990" checksum="CA59C718727D215E" modified="2013-07-24" version="1">MKTAIVGYPRIGAHRELKFASEQYFRGACTEAELQETAKSLRLANLTQQKESGLDWIPSNDFSFYDGMLDTAFLLNAVPKRYRELGLAPLDTYFAAARGYQGDKGDVKALAMKKWFNTNYHYMVPELEDDTAICLADEKPFNLYQEAKAAGVETKPVVIGAYTFLKLAVYNGKKTAADFVADTVKAYADLLQKFQTLGTAWVQFDEPCLVMDMTAEDKALFTQLYAGILAAKGSVKVLLQTYFGDVRDCYDTICKLDVDGIGLDFLEGKQSAKLVTEQGFPNDKILFAGVVNGKNIWKNHYDKTLSLLAELSQHAEQIVLNTSCSLLHVPYTTANEHKLSEEVLSHFAYAAEKLTELKELKQILSADHPQETAAYQKNAALFATPRSGANKAVQEQVAALKDADFVRLPAFAEREQIQKKTFGLPLLPTTTIGSFPQTAAVRANRAAFRKGEISQEQYDAFNKQQIADCVALQEKIGLDVLVHGEFERNDMVEYFGESLDGYVFTQNAWVQSYGTRCVKPPVIWGDVKRAKPMTVNWSVYAQSLTKKPMKGMLTGPVTILNWSFPREDISLKESAYQIALAIREEVLDLEANGITMIQVDEAALREKLPLRKSDWNSDYLDWAIPAFRLVHSGVKPETQIHTHMCYSEFTDIIAEIDDMDADVITFEASRSDLKILDSLQEHHFRTEVGPGVYDIHSPRVPSVEEIKLALHKMLEKIPVEKLWVNPDCGLKTRGDKETIPSLEHLVCAAQEVRCELSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0D6</accession>
    <name>R5V0D6_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Uroporphyrinogen-III C-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.107</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN566_01759</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the precorrin methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.107" evidence="1"/>
    <dbReference type="EMBL" id="CBAR010000180">
        <property type="protein sequence ID" value="CCZ84033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01759"/>
    <dbReference type="EnsemblBacteria" id="CCZ84033">
        <property type="protein sequence ID" value="CCZ84033"/>
        <property type="gene ID" value="BN566_01759"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004851">
        <property type="term" value="F:uroporphyrin-III C-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004852">
        <property type="term" value="F:uroporphyrinogen-III synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019354">
        <property type="term" value="P:siroheme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06578">
        <property type="entry name" value="HemD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11642">
        <property type="entry name" value="SUMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10090">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036108">
        <property type="entry name" value="4pyrrol_syn_uPrphyn_synt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003754">
        <property type="entry name" value="4pyrrol_synth_uPrphyn_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006366">
        <property type="entry name" value="CobA/CysG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003043">
        <property type="entry name" value="Uropor_MeTrfase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02602">
        <property type="entry name" value="HEM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="SSF53790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69618">
        <property type="entry name" value="SSF69618"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01469">
        <property type="entry name" value="cobA_cysG_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00840">
        <property type="entry name" value="SUMT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TP_methylase" evidence="7">
        <location>
            <begin position="5"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="HEM4" evidence="8">
        <location>
            <begin position="268"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ84033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="495" mass="53605" checksum="3DEB8A537CEA108B" modified="2013-07-24" version="1">MKKGMVYLVGAGSGNPALLTQKGQQCLQLADLVVYDALASVSILNHVRPDCELYFVGKRAGNHSLPQVEINQLLVDAAKQGKQVVRLKGGDPFVFGRGGEEAQALSAANVPFVIVPGVSASYGVPAYAGIPVTHRDYASSFHVITGHTQADPKQEPDYAELAKLNGTLVFLMGLANLSHITTALMENGMSADMPVAVIQEGTTARQRTVTGTLSTISDFVVQQHIQAPATIVVGKVVTLREQLQWFEQGKLFGKRILITGTPPHSRHLSEHFQQYGAETMEISLIDTVSIADAPLKSVDWSAYTWIVFSSANSVHIFFDSLQQHDIDLRMLLHLRFAAIGNGTACELAAKGIFVDCVPHRFESRYLAEALIPQLEPHDRVLLIRSKNGSTVLPDMLKAAKKSVDSISLYETKPLLQKETLLKQHLPDADYLVFSSSSAVQAFIQMYGTNLPDPVKVISIGAVTTKTAETLGIPVFATAKEATAEGIAACMLQDCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0D9</accession>
    <name>R5V0D9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Amidohydrolase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01764</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000181">
        <property type="protein sequence ID" value="CCZ84038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01764"/>
    <dbReference type="EnsemblBacteria" id="CCZ84038">
        <property type="protein sequence ID" value="CCZ84038"/>
        <property type="gene ID" value="BN566_01764"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032465">
        <property type="entry name" value="ACMSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21240">
        <property type="entry name" value="PTHR21240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04909">
        <property type="entry name" value="Amidohydro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="3">
        <location>
            <begin position="9"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="271" mass="31333" checksum="263C8EF761839D5D" modified="2013-07-24" version="1">MKNGYTIFDAHAHVFPNKIAERATSSISVFYDDMPMQHIGSMEQLLKSGANAGIDGYLICSTATNVAQVQAINQFLWDSCKDHVNCIAFAAMHPFAPDCQADMDFILQHGFRGIKLHPDFQKFYIDDPAVYPLYEIIQETQLPILMHMGDPHQTYSQPQRLAKVLKEFPKLRVIAAHLGGWERWEEAVANLKADERLRFDTSSCSPFLPKDKMREIIRHYGAENCFFGVDFPMWDHEEELERFFALSLTDYENKRILSENLIEWIHQTNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0F2</accession>
    <name>R5V0F2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative phage integrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_01784</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Site-specific tyrosine recombinase, which acts by catalyzing the cutting and rejoining of the recombining DNA molecules.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 'phage' integrase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000184">
        <property type="protein sequence ID" value="CCZ84058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01784"/>
    <dbReference type="EnsemblBacteria" id="CCZ84058">
        <property type="protein sequence ID" value="CCZ84058"/>
        <property type="gene ID" value="BN566_01784"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004107">
        <property type="entry name" value="Integrase_SAM-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14659">
        <property type="entry name" value="Phage_int_SAM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyr recombinase" evidence="5">
        <location>
            <begin position="179"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ84058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="370" mass="42849" checksum="BCDA5969E5D89A38" modified="2013-07-24" version="1">MGRPKKEKPNRKDGCYEIKATVGIADDGTPIRKSFYSTKSKEDAQRKAEQYKVQQELQEQTGICLQKNRTLFSAWAREWLETYKKGKVKDQTYYFTYQVNVEKYLIPFFGAMRLQMIRQIDVQRYFNTVQNAETGAALSASVLDKHKMILSAIFHAAIDNDLCFKNPVKNIKITSKPKQERHVYTAEQAKIAEQYAMKHGRYDIVLLMHTGLRRSELLGLQWGDLDFEKKLLHIQRAVTQTKGGILIDKPKTKTSIRAIPISDFVVAILEKIERTGFYVIAGKHPDEPMKPHTYADHFSDFMKRMQKETGLPILTPHELRHTFGTLLRENDVDIYTIQKVLGHSDITVTSNIYVHNDIEVLRKRLKVDEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0J5</accession>
    <name>R5V0J5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Pyruvate flavodoxin/ferredoxin oxidoreductase thiamine diP-binding domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01525</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000162">
        <property type="protein sequence ID" value="CCZ83790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01525"/>
    <dbReference type="EnsemblBacteria" id="CCZ83790">
        <property type="protein sequence ID" value="CCZ83790"/>
        <property type="gene ID" value="BN566_01525"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07034">
        <property type="entry name" value="TPP_PYR_PFOR_IOR-alpha_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033412">
        <property type="entry name" value="PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002880">
        <property type="entry name" value="Pyrv_Fd/Flavodoxin_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17147">
        <property type="entry name" value="PFOR_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01855">
        <property type="entry name" value="POR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="POR_N" evidence="1">
        <location>
            <begin position="14"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="PFOR_II" evidence="2">
        <location>
            <begin position="247"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="353" mass="38058" checksum="A03F59579D87BAF0" modified="2013-07-24" version="1">MERSLMKGNEALAEAAIRAGCKCFFGYPITPQTELSAYMAKKMQKAGGVFLQAESEIAAINMVLGATSTGVRAMTSSSSPGISLKAEGISYMAGSDLPGVIINVQRGGPGLGSIQPAQSDYYQATHAMGHGDFHIFVFAPSSVQEMVDMVTLAFDKADEYRMPAMILADGLLGQMMEPVTFPEPLETLPDKSDWATNGHQNKRAHHIINSLYLDPAELEAKVKERFKRYAIVEEKETRAESYLCDDAEIVVTAYGASARVAKSAVNTARKAGIKAGLFRPITLWPFPKKELQAATKSATHILDVEMSMGQMVDDVRLATECRIPVSFFGRTGGVIPTPGEVLAELKKLAGGAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0K5</accession>
    <name>R5V0K5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01849</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000187">
        <property type="protein sequence ID" value="CCZ84123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84123">
        <property type="protein sequence ID" value="CCZ84123"/>
        <property type="gene ID" value="BN566_01849"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="210" mass="23332" checksum="9134C69AC3257B61" modified="2013-07-24" version="1">MFFLIASCFVCANAANFFFSHQAVRVKSRIMQRQRTIADVLQVDTANSADSIREELFANRLCNAKGFKNLRALIRLDGGNTHLRGNLYDTADNCLNVCLACLRIILIQFAGVGHFIQGIQCQIRIDCTCTKPKQGCKVMDFGRFCSFYNQGNSGILSGAYQIGFQCGNCQQRRNCKVFLIHATVCQNQNRCSGIVCSICLCIQIGNGICQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0R8</accession>
    <name>R5V0R8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Thymidylate kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00170</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000168">
        <property type="protein sequence ID" value="CCZ83865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00170"/>
    <dbReference type="EnsemblBacteria" id="CCZ83865">
        <property type="protein sequence ID" value="CCZ83865"/>
        <property type="gene ID" value="BN566_00170"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039430">
        <property type="entry name" value="Thymidylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02223">
        <property type="entry name" value="Thymidylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thymidylate_kin" evidence="1">
        <location>
            <begin position="9"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="224" mass="25109" checksum="9C884557FFFBBC29" modified="2013-07-24" version="1">MPNGKLLVLDGLDGSGKSTQFELLQTALQKQGVSIQAVSFPDYDSPSAALVKMYLNGELSNTPDGVNAYAASSFYAVDRYANYHLHWEQAYHNGTCILASRYTTSNAIYQMGKLPKSEWDNYLQWLAEYEYGRLELPEPDTVIYLDMPVSVQQALLSKRYGGDAGKKDLHEADIAFQLQCQQAAYYAAEKLGWKVLPCSQNGEPLPVETIHQELFRHVQTSWGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1B9</accession>
    <name>R5V1B9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Porphobilinogen deaminase</fullName>
            <shortName evidence="6">PBG</shortName>
            <ecNumber evidence="6">2.5.1.61</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Hydroxymethylbilane synthase</fullName>
            <shortName evidence="6">HMBS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Pre-uroporphyrinogen synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">hemC</name>
        <name evidence="9" type="ORF">BN566_01756</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 6">Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>H2O + 4 porphobilinogen = hydroxymethylbilane + 4 NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:13185"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57845"/>
            <dbReference type="ChEBI" id="CHEBI:58126"/>
            <dbReference type="EC" id="2.5.1.61"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>dipyrromethane</name>
            <dbReference type="ChEBI" id="CHEBI:60342"/>
        </cofactor>
        <text evidence="6">Binds 1 dipyrromethane group covalently.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Monomer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="6">The porphobilinogen subunits are added to the dipyrromethane group.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the HMBS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.61" evidence="6"/>
    <dbReference type="EMBL" id="CBAR010000180">
        <property type="protein sequence ID" value="CCZ84030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01756"/>
    <dbReference type="EnsemblBacteria" id="CCZ84030">
        <property type="protein sequence ID" value="CCZ84030"/>
        <property type="gene ID" value="BN566_01756"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004418">
        <property type="term" value="F:hydroxymethylbilane synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018160">
        <property type="term" value="P:peptidyl-pyrromethane cofactor linkage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006779">
        <property type="term" value="P:porphyrin-containing compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00260">
        <property type="entry name" value="Porphobil_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000860">
        <property type="entry name" value="HemC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022419">
        <property type="entry name" value="Porphobilin_deaminase_cofac_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022417">
        <property type="entry name" value="Porphobilin_deaminase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022418">
        <property type="entry name" value="Porphobilinogen_deaminase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036803">
        <property type="entry name" value="Porphobilinogen_deaminase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11557">
        <property type="entry name" value="PTHR11557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01379">
        <property type="entry name" value="Porphobil_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03900">
        <property type="entry name" value="Porphobil_deamC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001438">
        <property type="entry name" value="4pyrrol_synth_OHMeBilane_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00151">
        <property type="entry name" value="PORPHBDMNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54782">
        <property type="entry name" value="SSF54782"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00212">
        <property type="entry name" value="hemC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00533">
        <property type="entry name" value="PORPHOBILINOGEN_DEAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Porphobil_deam" evidence="7">
        <location>
            <begin position="6"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="Porphobil_deamC" evidence="8">
        <location>
            <begin position="224"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="modified residue" description="S-(dipyrrolylmethanemethyl)cysteine" evidence="6">
        <location>
            <position position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ84030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="302" mass="33038" checksum="F1686CD65F54CEEE" modified="2013-07-24" version="1">MKPQRIRIGSRASRLAVIQSEWVMQQIHQIAPEIQLELVTMQTTGDKILHKTLDQIGGKGLFLKELDAALLANEVDLCVHSLKDVPVLENPALPIGAYPKRAPAYDGMVLPKQQSFADWNQSLPVGSASSRRTLQFQMQYPACTVHPIRGNVLTRLEKLDRGEYGALILAEAGLQRLGLEHRITRRFSPAEIVPAAGQGILAVQGRAGEYTDLLQRLNDPQAAFCAKIERAVSKALNGDCTAPIGAYADLQGEHVTLYGFYAEDGVGIKSTLTGSVTQAESLANTFAEQLKQQVQEKKEAML</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1D8</accession>
    <name>R5V1D8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ribosome maturation factor RimP</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rimP</name>
        <name evidence="6" type="ORF">BN566_00300</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Required for maturation of 30S ribosomal subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RimP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000223">
        <property type="protein sequence ID" value="CCZ84393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00300"/>
    <dbReference type="EnsemblBacteria" id="CCZ84393">
        <property type="protein sequence ID" value="CCZ84393"/>
        <property type="gene ID" value="BN566_00300"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01734">
        <property type="entry name" value="YlxS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01077">
        <property type="entry name" value="RimP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003728">
        <property type="entry name" value="Ribosome_maturation_RimP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028998">
        <property type="entry name" value="RimP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036847">
        <property type="entry name" value="RimP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028989">
        <property type="entry name" value="RimP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035956">
        <property type="entry name" value="RimP_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33867">
        <property type="entry name" value="PTHR33867"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02576">
        <property type="entry name" value="DUF150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17384">
        <property type="entry name" value="DUF150_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74942">
        <property type="entry name" value="SSF74942"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75420">
        <property type="entry name" value="SSF75420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="DUF150" evidence="4">
        <location>
            <begin position="17"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="DUF150_C" evidence="5">
        <location>
            <begin position="96"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ84393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="158" mass="18202" checksum="B23B44871741E200" modified="2013-07-24" version="1">MKLKAFGATEQRIYNAVKPIADEFGFQIWDVRFEKEGACWYLRIFIDQESGITIEDCEKMTEPVNKLLDEQDPISQNYILEVGSAGLEREFNRTWHFTDGIGTEVRARTIRPIDGEREFIGTLQSCKDDMLTLETETGSISLPLAALTFVKRYEAFEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1E3</accession>
    <name>R5V1E3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RNA polymerase alpha subunit domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01791</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000184">
        <property type="protein sequence ID" value="CCZ84065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01791"/>
    <dbReference type="EnsemblBacteria" id="CCZ84065">
        <property type="protein sequence ID" value="CCZ84065"/>
        <property type="gene ID" value="BN566_01791"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007630">
        <property type="entry name" value="RNA_pol_sigma70_r4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011260">
        <property type="entry name" value="RNAP_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03118">
        <property type="entry name" value="RNA_pol_A_CTD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04545">
        <property type="entry name" value="Sigma70_r4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNA_pol_A_CTD" evidence="2">
        <location>
            <begin position="92"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="RNA_pol_A_CTD" evidence="2">
        <location>
            <begin position="178"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4" evidence="3">
        <location>
            <begin position="385"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="998" mass="117219" checksum="64B402E9FF847134" modified="2013-07-24" version="1">MVSTSKNVDDSENKNDIDETPFRNDFSMLHFSSLPTIEDATYEVEHARLEENNIEETPFLITPNLTKQPIEENPKTVHVEKKKENAMPSTPEQYNLNISVQDLPLSLRSRNGLCRGGIMTLRQLLQYDKESLLGVRNLGKYSIKEITDFLATTFHHGKKKISGENLSNQPLYTKETYISEIDTIPISPRTVNALMRGGVDTIGKLMRANNERILSIRNLGKKSFEEALCLKKQIPKLLIQSNYTESDSSDDVKNTFEMMVAAFALTDEDEIEELRLYCYHFNDPEDTEQERLEKWYHLPCVKSVATRKIANALKEHSVFGLRLSELRAMLPENFPEVLFEEILDQVAQKDHGKRYVQKSESLHSMLEKENIMNAPEFEILKSRMKNKTLEEIANQYGLTRERIRQKQERALRRIRKIADCSNVMITENRFRPLFETYAFQEKLFCTLTEQSSEVYRFLEFSVKKKGIAPIEKMFQEVQVPGWMVGNWEKYCQKSIDSQYLCITEDGNRLIEKTRHGIISYLIAKYCQDEMLFDDFVKLYQGFIVNHGLEDDSRLCISEFEKRSQENQLSARMDIFWKIGRKFRYYDIDAHDYTELLETLDLNQYHNVKLSTLKFIREYPELMQQYDIRDEYELHNLLKKIADKIDAPELQFGRMPHLCFGEFDQIAMIKAHMWKLAPISKEALANAISEDYGFRPESLIGLFGCIDLYEKDGVYIVDHKAMTEEQIKILSENLPDDFYYFDEIEQKFAMLFPDAEIELVNGFNLKRLGFVLNTTYAVKNYPSSSSYFHALLTKNDITDISAMRKRFYKINMFSSILYELKRDYTIIEFEKNKYVSIARLESLGFDRTKLKQYCDKVYAYAQNIDFFTIESLSKQGFTADLESLGFEPWFYSSILREDHRFSHRKMGGTVLFHAAGQEASIRNLVFSILEQESSVDVDRLIAQLKEDYHIQLDRWDIIPRIQNTELYFDNIMDKIYRNYHTYYEELLSIDEEDEYEFIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1N9</accession>
    <name>R5V1N9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA threonylcarbamoyladenosine biosynthesis protein TsaE</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaE</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_00367</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TsaE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000238">
        <property type="protein sequence ID" value="CCZ84468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00367"/>
    <dbReference type="EnsemblBacteria" id="CCZ84468">
        <property type="protein sequence ID" value="CCZ84468"/>
        <property type="gene ID" value="BN566_00367"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002949">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003442">
        <property type="entry name" value="T6A_TsaE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02367">
        <property type="entry name" value="TsaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00150">
        <property type="entry name" value="T6A_YjeE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00032441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ84468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="162" mass="17474" checksum="AD7F2CDFC26E9A14" modified="2013-07-24" version="1">MQKIVIQTHSAAETMQLAEQMGSLLQAGDCIAYFGGLGAGKTTFTRGLAKGMGLPDEVASPTFAIVNEYHGTGALSLYHFDMYRIIGAESLETTGFYDYPLEENVFAIEWAENIADCLPENRIAVTIEPLSETDRQITIEGGTRFAVLGHGHFGQCGSSCTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1P0</accession>
    <name>R5V1P0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HD domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01920</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01920"/>
    <dbReference type="EnsemblBacteria" id="CCZ84195">
        <property type="protein sequence ID" value="CCZ84195"/>
        <property type="gene ID" value="BN566_01920"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="161" mass="18520" checksum="E2C5D6952006594F" modified="2013-07-24" version="1">MKSIETEVVALVERNGRLILQSAGMEQEKGYIQHGAVSCYLHSFFVAYMSVWLAKRFRIRVHLRSLVRGALLHDYFLYDWHIKDPNRKAHGFSHAAAALQNAERDFALNAVERDVIARHMFPLNLTPPRYRESILVCIADKICAVYETFSIPIPKHVLLAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5V282</accession>
    <name>R5V282_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00501</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000265">
        <property type="protein sequence ID" value="CCZ84648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84648">
        <property type="protein sequence ID" value="CCZ84648"/>
        <property type="gene ID" value="BN566_00501"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="105" mass="11074" checksum="1FF7D40C7D55DC3A" modified="2013-07-24" version="1">MKTRTIVSIILATATVVLGTIFFVTKLRKKHHDGCGCDFAGTDDCIGGEDCDCDGTDEPMCEAPCCDADACTDDEDDDADSCASMAADKVEPEAPSEPTVSEDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>R5V2C3</accession>
    <name>R5V2C3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">30S ribosomal protein S15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rpsO</name>
        <name evidence="8" type="ORF">BN566_00359</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Forms an intersubunit bridge (bridge B4) with the 23S rRNA of the 50S subunit in the ribosome.</text>
    </comment>
    <comment type="function">
        <text evidence="3 6">One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it helps nucleate assembly of the platform of the 30S subunit by binding and bridging several RNA helices of the 16S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Part of the 30S ribosomal subunit. Forms a bridge to the 50S subunit in the 70S ribosome, contacting the 23S rRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the universal ribosomal protein uS15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000237">
        <property type="protein sequence ID" value="CCZ84460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00359"/>
    <dbReference type="EnsemblBacteria" id="CCZ84460">
        <property type="protein sequence ID" value="CCZ84460"/>
        <property type="gene ID" value="BN566_00359"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00353">
        <property type="entry name" value="Ribosomal_S15p_S13e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01343_B">
        <property type="entry name" value="Ribosomal_S15_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000589">
        <property type="entry name" value="Ribosomal_S15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005290">
        <property type="entry name" value="Ribosomal_S15_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009068">
        <property type="entry name" value="S15_NS1_RNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23321">
        <property type="entry name" value="PTHR23321"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00312">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01387">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47060">
        <property type="entry name" value="SSF47060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00952">
        <property type="entry name" value="S15_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00362">
        <property type="entry name" value="RIBOSOMAL_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3 4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 6" id="KW-0699">rRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ84460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="88" mass="10458" checksum="178AFD91A7B90A4D" modified="2013-07-24" version="1">MLRKEEKDQIIEANRTHEHDTGSPEVQIAILTKRINDLTGHLKTHKKDHHSRRGLLKMVGHRRNLLNYLKKKDVERYRACIEKLGIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>R5V2Q2</accession>
    <name>R5V2Q2_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glutamyl-tRNA(Gln) amidotransferase subunit A</fullName>
            <shortName evidence="8">Glu-ADT subunit A</shortName>
            <ecNumber evidence="8">6.3.5.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">gatA</name>
        <name evidence="10" type="ORF">BN566_00411</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 8">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) + L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterotrimer of A, B and C subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the amidase family. GatA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.7" evidence="8"/>
    <dbReference type="EMBL" id="CBAR010000243">
        <property type="protein sequence ID" value="CCZ84550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00411"/>
    <dbReference type="EnsemblBacteria" id="CCZ84550">
        <property type="protein sequence ID" value="CCZ84550"/>
        <property type="gene ID" value="BN566_00411"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030956">
        <property type="term" value="C:glutamyl-tRNA(Gln) amidotransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00120">
        <property type="entry name" value="GatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000120">
        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004412">
        <property type="entry name" value="GatA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895">
        <property type="entry name" value="PTHR11895"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="SSF75304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00132">
        <property type="entry name" value="gatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Amidase" evidence="9">
        <location>
            <begin position="24"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8">
        <location>
            <position position="78"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="8">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ84550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="487" mass="51677" checksum="3C22D8D98EA261B2" modified="2013-07-24" version="1">MELYEQTAAKLSEMLQNKECSAVELCQSVQGRIAAVEDKVGAYVTQCEDALAQAEAVDQARAAGETLHPLAGIPIGIKDNISTKGLRTTCASKMLADYVPPFDATVMQPIRASKMVITGKLNMDEFAMGSSTETSYIKKTHNPHNLDCVPGGSSGGSAAAVAAGEAIVTLGSDTGGSIRQPSSFCGTVGLKPTYGSVSRYGLVAFASSLDQIGPIGKSVKDVAMLYSLICGHDNMDATSAYRDYPDFAAALQGSSVAGLKIGLPKEYFGAGIDDTVKAAVMDAVHQLEANGATVQEISLPSTEYAVNTYYIIASAEASSNLARFDGVKYGYRAKNYDGLTDMYERTRSEGFGDEVKRRIMLGTFVLSSGFYDAYYKKAKLVQRQIAQEFAAAFQQCDIIITPTYPTPAFRLGEQVDDPLKMYSGDVCTVPVNIAGLPAISVPCGKTASGLPIGMQMIGAKFAEQTLLRAADAYEQLCGGFACIPQTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-09-29" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5V3Y4</accession>
    <name>R5V3Y4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Dockerin-like protein F5/8 type C domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00629</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000011">
        <property type="protein sequence ID" value="CCZ82824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82824">
        <property type="protein sequence ID" value="CCZ82824"/>
        <property type="gene ID" value="BN566_00629"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002105">
        <property type="entry name" value="Dockerin_1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00404">
        <property type="entry name" value="Dockerin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004406719" evidence="1">
        <location>
            <begin position="29"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="2">
        <location>
            <begin position="615"/>
            <end position="684"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ82824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="687" mass="76586" checksum="7B98291B1F4234CD" modified="2013-07-24" version="1">MKKAMKCMAGILALTLAFAQVAPVSAFAEETTAATATEEAQAVYSGDCSAEGSSVTWTYNPTEKTLTFSGTGAIKDYQASGEALPWLSASDDYNVKKVVLEEGITSLPDFAEENGLFDLRKGGRPCTIILPESLTDFHYGTALSLSRGVILYVKDGSAAYCDVHAIADRNYFTNRNWLIYSSGVAENPVVPTEGTSDTGLTWKFDYETRQLTLSGTDDYQNSYLIQHLMPLMKAADKVVFDENFTVPEDPNETVMPATYTYLKKVLVDNPALQYFNSSQGFCCYYQSPFQTAYEEVKEAYEKQYPTTEEETNPFEYQCVVRTNPNLSTYSGNCGVEGGDNVTWTYDVATATMTFSGTGEMQGLYDVGTEKYTLPSWLYGYGAVPNYHPKHIIIEEGITRIVADRWYLFGHDIPSSESERCTVTIPESLKNTNLFDYAINPNDYLTFQVKQQSVFYFQLMNAMDLHPDNHWIYESTGLAKDVIVSEDGMTEGSSEKGLHWKFDAEKRVLYLSGTDVPGNQGSALSEIKDLVSVAKTIVIDKDFVPPLGTDLTTWTNYYLKSTSNRDIYHNVYLYRGSLFDQHYLAAKALYKEYEHLTDEEEERYGIFNQGYYYIGEADTCGDVNLDGTVDLVDAIYFGKYLAGLVQMSDAQLAATDCNGNGVTGEEADLSVLMKFLIGGITDLPYTEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5V436</accession>
    <name>R5V436_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ABC-type transport system involved in lipoprotein release permease component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00681</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000021">
        <property type="protein sequence ID" value="CCZ82879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00681"/>
    <dbReference type="EnsemblBacteria" id="CCZ82879">
        <property type="protein sequence ID" value="CCZ82879"/>
        <property type="gene ID" value="BN566_00681"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038766">
        <property type="entry name" value="Membrane_comp_ABC_pdt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30287">
        <property type="entry name" value="PTHR30287"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="244"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="287"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="333"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="372" mass="42765" checksum="C65E3A59DF7FC91A" modified="2013-07-24" version="1">MKFIRRDLKRHQKKKAFRLNSKIGILKRFRIRVIFQNLPNYITIVIGILFANFILMFGFMFEPMLDHFQDQITSNMIADYQYLLKAPQETENEEAEKYSATALKTLEENGKKSEEVSVYGIQSDSAYLDLDWKTKDDGVIISTAYANKLNVEEGDTITVKESYGDKEYTFHVDGVYDYPAAIAMFMPQESFNKTFDFDADSFNGYFSNTEITDIDDLYVATVITVDDMTKTSRQLKVSMGNMMAIFYVFGIIMFMLIVYLLSKIIIEKNAQSISMTKILGYRNSEINGLYVLPTSIVVIASMILTIPICHFIMKYVCIQVFASYPGWLEYYVPFYVFVKMVILGICGYAVIAFLQNRKVKKVPLGEALKNAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>R5V4L4</accession>
    <name>R5V4L4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Superfamily II DNA and RNA helicases</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BN566_00812</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the DEAD box helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000039">
        <property type="protein sequence ID" value="CCZ83019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00812"/>
    <dbReference type="EnsemblBacteria" id="CCZ83019">
        <property type="protein sequence ID" value="CCZ83019"/>
        <property type="gene ID" value="BN566_00812"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005580">
        <property type="entry name" value="DbpA/CsdA_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03880">
        <property type="entry name" value="DbpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Q_MOTIF" evidence="11">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="32"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="228"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="532"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="6">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CCZ83019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="570" mass="63051" checksum="B3645A1D314ECBAB" modified="2013-07-24" version="1">MQFQNTTLSAEMLQAIATQNFTEMTEIQAKTIPLLLEGKDVIGKSNTGTGKTAAFSIPIIEQIPKDAEPFVYALILCPTRELAMQACEEIGKFAKFKRWVKPVAVYGGASMERQIHALKNGANIVVGTPGRVLDHLRRRTLKLEHLRTIVLDEADEMLNMGFREDIESVLAQTPEDRQTVLFSATMPPAILAITKQYQKDPQMVQIQSPHRTVDAIAQYAINVPMGRKTDALHLLLASKKPVSSMVFCNTKKMVDELTEALQNRGFAAVGLHGDMKQMQRTQVMNSFKNGRCNILIATDVAARGIDVKGVDAVFNYDLPQDHEYYIHRIGRTGRAGKEGTAYNIISGRKQEQELRAISQYTRANIEELNLPTSAEMKQEALDQLQQRLQEQCSSAAPSEEAVQLAEQLIAAGTPAETLLALLLQEEITAATKNLPAFDIPKPIRKGKRSGAATVKLHINVGRKERMAPNFILGALVEATELSGSEFGKIDIYDKFTTVEVPAAEASNIVASMQSTKINGHAVKVKLFEERSNYRSGGGHSGNGRSDRFRRDHAYSSKNNSGSYNRKKRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5V4P4</accession>
    <name>R5V4P4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00852</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000048">
        <property type="protein sequence ID" value="CCZ83064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83064">
        <property type="protein sequence ID" value="CCZ83064"/>
        <property type="gene ID" value="BN566_00852"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010380">
        <property type="entry name" value="DUF975"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40076">
        <property type="entry name" value="PTHR40076"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="138"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="199"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="226"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="268" mass="29837" checksum="983245FF8A8BE42B" modified="2013-07-24" version="1">MSWQDWQNFQNWQNAQQQGQKAANNINLSNGVQKLEESDSFMFRSLGLTLKTLMKKPVQLWGLSLLGVILQSLSNVLCAVPPILGMGVSSTLSAGMENVFLDAYHQKEINSKQIFSGFSSGKSFLRIAGGMLWRTLWLFLWALFALIPIVGWVIAPIMLVIKGISYSFTPYLLLRKKDISPLDALKRSMEMTKGYRGKIFLTYLICYAALLVVGLLLFLIAKIGGFAVVLAGLIGLVIGIFCPLFFGVLRAAMFTEVVRLEKVTDIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>R5V4U0</accession>
    <name>R5V4U0_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Aldehyde-alcohol dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_00911</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the iron-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000060">
        <property type="protein sequence ID" value="CCZ83124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00911"/>
    <dbReference type="EnsemblBacteria" id="CCZ83124">
        <property type="protein sequence ID" value="CCZ83124"/>
        <property type="gene ID" value="BN566_00911"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008774">
        <property type="term" value="F:acetaldehyde dehydrogenase (acetylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006066">
        <property type="term" value="P:alcohol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015976">
        <property type="term" value="P:carbon utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08178">
        <property type="entry name" value="AAD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034789">
        <property type="entry name" value="AAD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001670">
        <property type="entry name" value="ADH_Fe/GldA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018211">
        <property type="entry name" value="ADH_Fe_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039697">
        <property type="entry name" value="Alcohol_dehydrogenase_Fe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012079">
        <property type="entry name" value="Bifunc_Ald-ADH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11496">
        <property type="entry name" value="PTHR11496"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00465">
        <property type="entry name" value="Fe-ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000111">
        <property type="entry name" value="ALDH_ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00913">
        <property type="entry name" value="ADH_IRON_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00060">
        <property type="entry name" value="ADH_IRON_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldedh" evidence="3">
        <location>
            <begin position="19"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="domain" description="Fe-ADH" evidence="4">
        <location>
            <begin position="465"/>
            <end position="857"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR000111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="872" mass="94742" checksum="03916DEB5BDFCFB4" modified="2013-07-24" version="1">MTEKTEQMTVGLVDTVSALEEKLAALRTAQAKFATYTQEQVDCIFRAAALAANQARIPLAKMAVAETGMGVVEDKVIKNNYAAEYIYNAYKDTKTCGVIEEDKAFGTKRIAEPIGVIAAVIPTTNPTSTAIFKTLISLKTRNAVLISPHPRAKKSTIAAAKIVLDAAVAAGAPKDIIGWIDVPSLELTNTLMQEADLILATGGPGMVRAAYSSGKPALGVGAGNTPAVIDKTADILLAVNSIVHSKTFDNGMICASEQSVIVDDSIYDQVKAEFIKTGCYVLTPEETEKVRKTILINGALNSKIVGQPAAKIAELAGVTVDPRTKVLIGEVESVDLEEEFAHEKLSPVLAMYHAADFADALNKADQLVQDGGYGHTASLYIDTVHERERLDQFAARMKACRIVVNTPSSHGGIGDLYNFNLAPSLTLGCGSWGGNSVSENVGVKHLLNIKTVAERRENMLWFRTPQKVYFKKGCLPVAMRELKQVLGKQKAFVVTDRFLFQSGTVHCITDQLHELGIAYQIFSDVEPDPTLACAKRGAEEMRAFAPDVIIALGGGSAMDAAKIMWVMYEHPEADFMDMAMRFLDIRKRVYTFPTMGEKAYFIAVPTSSGTGSEVTPFAVITDEKTGVKYPLADYALLPNMAIVDTDLMMTQPKGLTAASGIDALVHALEAYASVMATDFTDGLALRAIQNIFTYLPACYDNGLNEPVAREKMANASTMAGMAFANAFLGVCHSMAHKLGAFHHLPHGVANAVLLTDVMRYNADPRPQKMGTFSQYAYPHTLERYAEVARFLGLSGNSDEELLEQLIAKIEALKERVGIKKTIRDYGVKEEDFLATLDEMVEQAFDDQCTGANPRYPLMSEIKAMYLKAYYGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5V5E3</accession>
    <name>R5V5E3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NYN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01130</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000092">
        <property type="protein sequence ID" value="CCZ83354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83354">
        <property type="protein sequence ID" value="CCZ83354"/>
        <property type="gene ID" value="BN566_01130"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021139">
        <property type="entry name" value="NYN_MARF1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01936">
        <property type="entry name" value="NYN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NYN" evidence="1">
        <location>
            <begin position="223"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="441" mass="51897" checksum="FA1A73145B9E1CEF" modified="2013-07-24" version="1">MTVDTKEVVTTIAYMIGVRMSALTASYGECAELIDKLKADRDATAIRYLCKLRTALMQKFKKTDDLIRYQLKNLHNIEWYDHENIKQLEKWGFTIIQANSRSEKYMQEFTRLINENIDKCSRLFYDWLNWEYIRDLFFVPKYNKNGVMKEEFNKYMANIEHYPFQMYIHWQPAEVGSIVYSDRKFLKIIYGQHNDSFTDYTKYRDADDETRNNIYNFIDSAEKTAVAVDCENSNPYKLYSVLKGLNQEELAKIEKITLYDDPNTTAGWDWLSKFTQIPVEHIEIDRVTDRKSLVDLRMTASVVTDFYRDGITSFIIVSSDSDFWGLIESLPKAHFLVMYEYEKCGTSIKNALTQHGIYYCAIDDFCSAATEDMKRAVLFAELEKHLPTLYGENPLELTQKIYEATRVTATKKEMENFCNRYVKTLRLKLNSEGKFIIEIQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5V5J7</accession>
    <name>R5V5J7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">N-acetylmuramoyl-L-alanine amidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01180</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000096">
        <property type="protein sequence ID" value="CCZ83404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01180"/>
    <dbReference type="EnsemblBacteria" id="CCZ83404">
        <property type="protein sequence ID" value="CCZ83404"/>
        <property type="gene ID" value="BN566_01180"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008745">
        <property type="term" value="F:N-acetylmuramoyl-L-alanine amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009253">
        <property type="term" value="P:peptidoglycan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02696">
        <property type="entry name" value="MurNAc-LAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002508">
        <property type="entry name" value="MurNAc-LAA_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01520">
        <property type="entry name" value="Amidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00646">
        <property type="entry name" value="Ami_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="MurNAc-LAA" evidence="2">
        <location>
            <begin position="124"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="248" mass="27259" checksum="2342F725016E76EC" modified="2013-07-24" version="1">MKSLTKLVLRKSVIVVLACVCGVTAVYFGGQKAEEAVQTLATRPPYEPPTIVLDAGHGGMDGGCTSVNGVSEKGINLEILLHLRAMLQMEGYTVVVTRDTDRSIHEEGVEGIANQKSSDMDHRLALFNEYDPAVCVSIHQNQFTDAAYSGAQMFYSSTNRDNSILAQALQDAFVAQLQPENKREIKQCGKELFLCYFSENPTVMAECGFLSNPEEAALLESTEYQQKVAFTLFDGLNQYLAEKQRKAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5V5M3</accession>
    <name>R5V5M3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01203</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000102">
        <property type="protein sequence ID" value="CCZ83429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83429">
        <property type="protein sequence ID" value="CCZ83429"/>
        <property type="gene ID" value="BN566_01203"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="279" mass="31551" checksum="51D693D5DD3E63E8" modified="2013-07-24" version="1">MICPACGSEFPDEMPVCPYCQTHVPEHTPDVHAYDAYYCADTVSLRKNHWIDFFIGLLFVIALTVLAIFFQLQKPSLSTQIRRADAAELAEICTEYPAETADDAYTQTLLNAIADLQQTYLLHDDQESDVLENLQKLAATENVLVREQACDAAAEMESNRLLQEMNRVRTQRQKRMLTESDTLTETAKLLVDTYQESPDTYEAEAPKAVKESLGEQAEQAYQVMLVNCNSYTDAIAQYNEERKDVTVNFLTTQDYTQVGISSIYDPVGKQFSFIVLLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5V614</accession>
    <name>R5V614_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cell wall-binding repeat protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01303</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000121">
        <property type="protein sequence ID" value="CCZ83534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83534">
        <property type="protein sequence ID" value="CCZ83534"/>
        <property type="gene ID" value="BN566_01303"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003737">
        <property type="entry name" value="GlcNAc_PI_deacetylase-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024078">
        <property type="entry name" value="LmbE-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02585">
        <property type="entry name" value="PIG-L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102588">
        <property type="entry name" value="SSF102588"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="247" mass="28406" checksum="67EAD94CC51A2407" modified="2013-07-24" version="1">MGKQNRIRIGKRTIAILLAGILCLLSGFVIWYQNYYDIPALTMTDLAQLDLSQAKKLMIVAHPDDETLWGGAHLLEGDYLIVCLTHGSDAVRSAELRSIAEATGNACLILKYPDKTFGRRDDWSNVEEQIEQDITLILEDKPWESVVTHNKAGEYGHIHHKMTHELVTEAYQHEQLQMPLYCFGTYYSAKRLPEFEAQLPRISEEDLQKKEALLQNYKSQERVVEHLSHMNPYENWTLYDPAEEAES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5V617</accession>
    <name>R5V617_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Segregation and condensation protein B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01308</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000121">
        <property type="protein sequence ID" value="CCZ83539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01308"/>
    <dbReference type="EnsemblBacteria" id="CCZ83539">
        <property type="protein sequence ID" value="CCZ83539"/>
        <property type="gene ID" value="BN566_01308"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051304">
        <property type="term" value="P:chromosome separation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005234">
        <property type="entry name" value="ScpB_csome_segregation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34298">
        <property type="entry name" value="PTHR34298"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04079">
        <property type="entry name" value="SMC_ScpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019345">
        <property type="entry name" value="ScpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00281">
        <property type="entry name" value="TIGR00281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="2" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="176" mass="19158" checksum="1F64322AE5E60F2D" modified="2013-07-24" version="1">MLAPACAILEAILFASGDPIPLSRLAAASGIPERELPQLLNDLNTAYQKSGHALQVLLLEKTAQLCTVERYAPYIQAALEQKRTLPLTPAALEVLTIAAYHQPVTKRFIAHVRGIDSSTLVNTLVERGLLEEGERLDLPGKPIAYRTTPAFLRCFGLSSLDDLPPLPEIPVPEQTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>R5V648</accession>
    <name>R5V648_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AAA ATPase central domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01352</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CbxX/CfxQ family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000133">
        <property type="protein sequence ID" value="CCZ83584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01352"/>
    <dbReference type="EnsemblBacteria" id="CCZ83584">
        <property type="protein sequence ID" value="CCZ83584"/>
        <property type="gene ID" value="BN566_01352"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041627">
        <property type="entry name" value="AAA_lid_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000641">
        <property type="entry name" value="CbxX/CfxQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17866">
        <property type="entry name" value="AAA_lid_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00819">
        <property type="entry name" value="CBXCFQXSUPER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="2">
        <location>
            <begin position="6"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="230" mass="25601" checksum="2B872B1FCEE97FD6" modified="2013-07-24" version="1">MPPLQVSLHMVFSGNPGTGKTTVARLLSEIYAGLGVLSKGHLVETDRAGLVSGYVGQTAIRTKKVIQKALGGVLFIDEAYTLTSAAGSNDFGMEAVNTLLKEMEDHRDDLVVIVAGYPAEMEQFLDSNPGLRSRFRKVIYFADYTPEELLTIFQHICEKSALEPTAEANFYVLQYFRKRCAEKSESFANGREVRNFFDTALTRQANRLADCEEITTEMLTTLTLDDVKIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>R5V666</accession>
    <name>R5V666_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">S-adenosylmethionine decarboxylase proenzyme</fullName>
            <shortName evidence="10">AdoMetDC</shortName>
            <shortName evidence="10">SAMDC</shortName>
            <ecNumber evidence="10">4.1.1.50</ecNumber>
        </recommendedName>
        <component>
            <recommendedName>
                <fullName evidence="10">S-adenosylmethionine decarboxylase beta chain</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="10">S-adenosylmethionine decarboxylase alpha chain</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="10" type="primary">speD</name>
        <name evidence="11" type="ORF">BN566_01372</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the decarboxylation of S-adenosylmethionine to S-adenosylmethioninamine (dcAdoMet), the propylamine donor required for the synthesis of the polyamines spermine and spermidine from the diamine putrescine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>H(+) + S-adenosyl-L-methionine = CO2 + S-adenosyl 3-(methylsulfanyl)propylamine</text>
            <dbReference type="Rhea" id="RHEA:15981"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57443"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="4.1.1.50"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </cofactor>
        <text evidence="10">Binds 1 pyruvoyl group covalently per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Amine and polyamine biosynthesis; S-adenosylmethioninamine biosynthesis; S-adenosylmethioninamine from S-adenosyl-L-methionine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterooctamer of four alpha and four beta chains arranged as a tetramer of alpha/beta heterodimers.</text>
    </comment>
    <comment type="PTM">
        <text evidence="10">Is synthesized initially as an inactive proenzyme. Formation of the active enzyme involves a self-maturation process in which the active site pyruvoyl group is generated from an internal serine residue via an autocatalytic post-translational modification. Two non-identical subunits are generated from the proenzyme in this reaction, and the pyruvate is formed at the N-terminus of the alpha chain, which is derived from the carboxyl end of the proenzyme. The post-translation cleavage follows an unusual pathway, termed non-hydrolytic serinolysis, in which the side chain hydroxyl group of the serine supplies its oxygen atom to form the C-terminus of the beta chain, while the remainder of the serine residue undergoes an oxidative deamination to produce ammonia and the pyruvoyl group blocking the N-terminus of the alpha chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the prokaryotic AdoMetDC family. Type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.50" evidence="10"/>
    <dbReference type="EMBL" id="CBAR010000145">
        <property type="protein sequence ID" value="CCZ83609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01372"/>
    <dbReference type="EnsemblBacteria" id="CCZ83609">
        <property type="protein sequence ID" value="CCZ83609"/>
        <property type="gene ID" value="BN566_01372"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00331">
        <property type="reaction ID" value="UER00451"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004014">
        <property type="term" value="F:adenosylmethionine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006557">
        <property type="term" value="P:S-adenosylmethioninamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008295">
        <property type="term" value="P:spermidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00465">
        <property type="entry name" value="AdoMetDC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003826">
        <property type="entry name" value="AdoMetDC_fam_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009165">
        <property type="entry name" value="S-AdoMet_deCO2ase_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016067">
        <property type="entry name" value="S-AdoMet_deCO2ase_core"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33866">
        <property type="entry name" value="PTHR33866"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02675">
        <property type="entry name" value="AdoMet_dc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001356">
        <property type="entry name" value="SAM_decarboxylas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56276">
        <property type="entry name" value="SSF56276"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03331">
        <property type="entry name" value="SAM_DCase_Eco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="2 10" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="7 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 10" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="9 10" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="8 10" id="KW-0704">Schiff base</keyword>
    <keyword evidence="4 10" id="KW-0745">Spermidine biosynthesis</keyword>
    <keyword evidence="6 10" id="KW-0865">Zymogen</keyword>
    <feature type="chain" id="PRO_5023283983" description="S-adenosylmethionine decarboxylase beta chain" evidence="10">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023283982" description="S-adenosylmethionine decarboxylase alpha chain" evidence="10">
        <location>
            <begin position="118"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; via pyruvic acid" evidence="10">
        <location>
            <position position="118"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for processing activity" evidence="10">
        <location>
            <position position="123"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor; for catalytic activity" evidence="10">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage (non-hydrolytic); by autolysis" evidence="10">
        <location>
            <begin position="117"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="modified residue" description="Pyruvic acid (Ser); by autocatalysis" evidence="10">
        <location>
            <position position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ83609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="269" mass="31392" checksum="AE7B0D8379B01D67" modified="2013-07-24" version="1">MVGLEKKLALYGFNNLTKTLSFNIFDVCYAKTEREKQDYIRYIDEQYNSERLTNILCDVTEMIGASILNISKQDYEPQGASVNILIAEGHVPSQIDVSCNQGETFLKRRDIHAHLDKSHVTVHTFPESHPDNEVTTFRVDIDVSTCGEISPLNTLDYLIRSFDSDIITIDYRVRGFTRDVNGKKCFIDHNITSIQDYIDPEILLRYDTMDINMYQANIFHCRMLIKEMQLQNYLFKTDVYELDPRVRLDITNQLRREMIEIFSGRNIYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5V748</accession>
    <name>R5V748_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Pyrophosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01653</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000173">
        <property type="protein sequence ID" value="CCZ83924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01653"/>
    <dbReference type="EnsemblBacteria" id="CCZ83924">
        <property type="protein sequence ID" value="CCZ83924"/>
        <property type="gene ID" value="BN566_01653"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="101" mass="11637" checksum="E8208713BB7F55D1" modified="2013-07-24" version="1">MKINEMQKEIYQNKLNKGFNVTDVHEEFCLLYGEVAEAYDAWRKNKTTVGEELADIAIYLMGLSEILDIDLETEIEKKMAINKDRVYQLVDGVLTKTSSDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7H7</accession>
    <name>R5V7H7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Precorrin-2 dehydrogenase</fullName>
            <ecNumber evidence="2">1.3.1.76</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_01760</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; siroheme biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.76" evidence="2"/>
    <dbReference type="EMBL" id="CBAR010000181">
        <property type="protein sequence ID" value="CCZ84034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01760"/>
    <dbReference type="EnsemblBacteria" id="CCZ84034">
        <property type="protein sequence ID" value="CCZ84034"/>
        <property type="gene ID" value="BN566_01760"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00262">
        <property type="reaction ID" value="UER00222"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004325">
        <property type="term" value="F:ferrochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043115">
        <property type="term" value="F:precorrin-2 dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016852">
        <property type="term" value="F:sirohydrochlorin cobaltochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019251">
        <property type="term" value="P:anaerobic cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019354">
        <property type="term" value="P:siroheme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010388">
        <property type="entry name" value="Anaerobic_Co-chelatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028161">
        <property type="entry name" value="Met8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35330">
        <property type="entry name" value="PTHR35330"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06180">
        <property type="entry name" value="CbiK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ84034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="420" mass="47276" checksum="78B0745747B38679" modified="2013-07-24" version="1">MTVLAPEPIPLPLQHQSLRYIQAVYATSFLKDRKLVFVATNDIQLNQTIVREAQERGILVSSVTDGITTDFSVPAHTTTGNLTAAISTNGGSPSLAAAICKEVTPILSTYEPLCSLQVQIREEWKEWIFDARKRKQLLSSLSTPEALACYREQGTAAYLNHIKNPCRTAIVTVSVGTREKEEEEKTIVAIEKQMQAAFPNCDVYPAYSSRIILQKRKEQGEKVHSVTEVLEYLQKAGYQNVYCQPLYLTTGKIDAQFSADVQPFQKVFSIFKVGKPLLESQEAIPAFWNVIQHICSCSDVAYLWMGHGPHLTYQILKDWIQQQGYFNVFLATLKGEPSFDVVLREIQHTNYRKVVLVPLMLVAGWHAQTDMIGAENPNSWEQVLKRYGYQTEAILQGLGADSTIQQLYVKRIRALMASNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7H8</accession>
    <name>R5V7H8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">Pyruvate kinase</fullName>
            <ecNumber evidence="4 13">2.7.1.40</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">BN566_01765</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + pyruvate = ADP + H(+) + phosphoenolpyruvate</text>
            <dbReference type="Rhea" id="RHEA:18157"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.40"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the pyruvate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.40" evidence="4 13"/>
    <dbReference type="EMBL" id="CBAR010000181">
        <property type="protein sequence ID" value="CCZ84039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01765"/>
    <dbReference type="EnsemblBacteria" id="CCZ84039">
        <property type="protein sequence ID" value="CCZ84039"/>
        <property type="gene ID" value="BN566_01765"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00188"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030955">
        <property type="term" value="F:potassium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004743">
        <property type="term" value="F:pyruvate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.33.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001697">
        <property type="entry name" value="Pyr_Knase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_Kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018209">
        <property type="entry name" value="Pyrv_Knase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015793">
        <property type="entry name" value="Pyrv_Knase_brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015795">
        <property type="entry name" value="Pyrv_Knase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036918">
        <property type="entry name" value="Pyrv_Knase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015806">
        <property type="entry name" value="Pyrv_Knase_insert_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11817">
        <property type="entry name" value="PTHR11817"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00224">
        <property type="entry name" value="PK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02887">
        <property type="entry name" value="PK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01050">
        <property type="entry name" value="PYRUVTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="SSF50800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="SSF51621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52935">
        <property type="entry name" value="SSF52935"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01064">
        <property type="entry name" value="pyruv_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00110">
        <property type="entry name" value="PYRUVATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 13" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="8 13 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12 16" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PK" evidence="14">
        <location>
            <begin position="2"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="PK_C" evidence="15">
        <location>
            <begin position="356"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CCZ84039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="475" mass="52605" checksum="5B3DC6B77E67CD7A" modified="2013-07-24" version="1">MMRKTKIVCTIGPATDDDDIMRRIMQAGMNVARFNFSHGDYETHQRRYEQVVRLREELGLPIATLMDTRGPEIRLGKFVDDKPVTIHDGDTYVLTTEDVLCTAERGSVSFKNLPHDVKPGTHILINDGVIEMVVNRVNSTEITCLVIHGGVLSNNKGINVPGVTLSMPYLSEKDRNDLEFGAKMGFDFIAASFVSTSADIDYLRKYTHSLGWFNVRIIAKIENLEGVKNIDEILESADGIMVARGDLGVEIPFEQIPAVQKELIRKGYQAGKQVITATQMLESMITNSRPTRAEITDVANAIYDGTSAIMLSGETAAGSYPVEVVRTMDLIAKTTEDNIDYKHMHATYQYNKDISNAIAHATVTTAHDLDAKAILTVTMSGVTAREISKYRPKCPIISCTISDQVWRQMNLSWGVHPLMIGEKQNTDELFEAAVDAALENHLVDKDDIVVITAGVPVGVSHSTNMMKVERVDKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5V8C4</accession>
    <name>R5V8C4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00281</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000222">
        <property type="protein sequence ID" value="CCZ84374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00281"/>
    <dbReference type="EnsemblBacteria" id="CCZ84374">
        <property type="protein sequence ID" value="CCZ84374"/>
        <property type="gene ID" value="BN566_00281"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="235"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="269"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="389"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="417"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="443"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="476"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="521"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="569"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="608"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="650"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="679"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="707"/>
            <end position="731"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="751" mass="84135" checksum="F99E453CBD92DE2D" modified="2013-07-24" version="1">MKEMNEKELPKLRRGGLFVVALVLLIFVLLDAFRILSMGVYQGSLALIGVAGLLFLATKVIRRRKESRTLRFFGRMVIISTILELTLFQLPSYSLLSGGYEQKTFLPTSASISGGESTIDETGGAVTVTGKDQVIMEFQQLDMPVGTIQVDVDYGEKTKRAQLKIDMADETHVDYRYDIASGVVVKDNDNSNYTACRFSGDVSQLRLKVSCFNDNDSVTIQAIHFNKTIPFDISAIRFGFLTLVTTFCYGIVVSSFLKKPFRETRKSTTASVLALTGAAVLLATSIIMIKLPEDGFASRWKLEAGNQITQELVDAFENKQVNLLKEPTEQLINMENPYDWSARNQEGVSAEWDHVYYDGKYYSYYGIAPVLTFFLPYHKLTGHYFACDMAVWIFSCIGLVFLGLTYLAIAKRWFRDVPAGCVIGGFVILLTACGIWYSVGRTIFYEIAMSCGFMYLNAGAYFLISSNMLCDGKTSFVRTALCSLFLGFAVLSRPTLAVYAICAAVFLLMSIKSSSSTIKGRILFILCAAVPICVLGGFQMWYNAARFGSPFDFGIKYSLTINDFVHSQFHLVFVLIVLFNFLFAVPGFSTDFPYIKTPFSSFHANGYYFADAGNTSGIFFLALPVFGYLLSGRALRRLPDRKSRLRYGTLVGLPCVIMPLIIICSIWESGYAIRYTADFSWEIIIGALAVLFWLYRMSTNETKKTWFRGFLACSVMLALVINIVQIIPFAFSENDYPEVCRAMQDVIAFWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-09-29" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5V904</accession>
    <name>R5V904_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00440</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000247">
        <property type="protein sequence ID" value="CCZ84579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84579">
        <property type="protein sequence ID" value="CCZ84579"/>
        <property type="gene ID" value="BN566_00440"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004395014" evidence="1">
        <location>
            <begin position="25"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="207" mass="22138" checksum="04DD4AFB0D15BA9E" modified="2013-07-24" version="1">MKISHLVLCGGLTLALLLSGCADSDTKTTSKKTVPRTTVVTTIIPGEVGTDQNVDGATVTLNGVYRSSITDVHNGQNCNAIFFSFTVSNQTDAPISANFLQTCQIQLDGKPFDAFTIQSMRATYQQFGQDVEDFSQPIPAGATVTGYIGAEVYEDYQEGVLLYSPLAGGSGDNQDDSKLISYTFHPDDLLDIESAPMQTAMPTESNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5V909</accession>
    <name>R5V909_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted xylanase/chitin deacetylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00445</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000247">
        <property type="protein sequence ID" value="CCZ84584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00445"/>
    <dbReference type="EnsemblBacteria" id="CCZ84584">
        <property type="protein sequence ID" value="CCZ84584"/>
        <property type="gene ID" value="BN566_00445"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045493">
        <property type="term" value="P:xylan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014235">
        <property type="entry name" value="Spore_PdaA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10587:SF78">
        <property type="entry name" value="PTHR10587:SF78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="SSF88713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51677">
        <property type="entry name" value="NODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0858">Xylan degradation</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004406854" evidence="1">
        <location>
            <begin position="27"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="NodB homology" evidence="2">
        <location>
            <begin position="68"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="247" mass="27258" checksum="7D14643C2229F296" modified="2013-07-24" version="1">MNLKKTIAGCCALFLLYSMPLHTAALDSTCIGYGQGKATDSQNCPLDALAFNERYAEYGAFATTPDTSRIILTFDQGYENGYTAQILDTLKEKHATAIFFLTGDYAKKETALVERMIAEGHVLGNHGMTHASLPNCTQAEAMEEIQSLHDYVLQTYDYEMQYFRCPCGEYSEQALQWVQDAGYQTVFWSGAYVDWKTDDQPNPDTAYETLLSTAHGGEILLLHSVSSTNAAILGDVIDGFRAAGYTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5VGQ3</accession>
    <name>R5VGQ3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00566</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000004">
        <property type="protein sequence ID" value="CCZ82761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00566"/>
    <dbReference type="EnsemblBacteria" id="CCZ82761">
        <property type="protein sequence ID" value="CCZ82761"/>
        <property type="gene ID" value="BN566_00566"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ82761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="215" mass="23980" checksum="869ECF2D43DFB43E" modified="2013-07-24" version="1">MKQIVFDMDGVLFATEALSDRLWKEMMEERGTPEMTAALQQCRGRNRTDTRAFFASHYPDFPYIAMETEKRNRMLAALDKDGMPTMKGAESLLQALQDAGWSVALATSTSRKTTMHHLELSGFTRYFSVIVTGDEIQHGKPNPEIYLTACKKLHSKPEETYAVEDSENGGTSACAAGMRVLLVPDQAILSESLQQRAEAVLPSLCEVQERLLGTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5VHC4</accession>
    <name>R5VHC4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00746</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000030">
        <property type="protein sequence ID" value="CCZ82946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82946">
        <property type="protein sequence ID" value="CCZ82946"/>
        <property type="gene ID" value="BN566_00746"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024529">
        <property type="entry name" value="ECF_trnsprt_substrate-spec"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12822">
        <property type="entry name" value="ECF_trnsprt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="165"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="212" mass="21979" checksum="380E8E084F7F6D7D" modified="2013-07-24" version="1">MKSQKNIKSMVLLGLMAAVLVILAYTPLGYLNIGPLAITFNTIPVAIAAIALGPVGGIVAGGVFGLTSFLQCFGGSVLGTTLFGISPILAVFQCFVPRMLDGLLIGLLSNAMRKHCTSAIANCAVTGFFAAFLNTLFYMSSLILLFGKTDIILSYRETLAPGKNVFLFVCAFVGINAIAEWVSSTLVTGAVGAALTKAKLVPAPKAKLAAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>R5VHV1</accession>
    <name>R5VHV1_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ion-translocating oxidoreductase complex subunit A</fullName>
            <ecNumber evidence="8">7.-.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Rnf electron transport complex subunit A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rnfA</name>
        <name evidence="9" type="ORF">BN566_00884</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Part of a membrane-bound complex that couples electron transfer with translocation of ions across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">The complex is composed of six subunits: RnfA, RnfB, RnfC, RnfD, RnfE and RnfG.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the NqrDE/RnfAE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.-.-.-" evidence="8"/>
    <dbReference type="EMBL" id="CBAR010000054">
        <property type="protein sequence ID" value="CCZ83096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00884"/>
    <dbReference type="EnsemblBacteria" id="CCZ83096">
        <property type="protein sequence ID" value="CCZ83096"/>
        <property type="gene ID" value="BN566_00884"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022900">
        <property type="term" value="P:electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00459">
        <property type="entry name" value="RsxA_RnfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011293">
        <property type="entry name" value="Ion_transpt_RnfA/RsxA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003667">
        <property type="entry name" value="NqrDE/RnfAE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02508">
        <property type="entry name" value="Rnf-Nqr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006102">
        <property type="entry name" value="NQR_DE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01943">
        <property type="entry name" value="rnfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="103"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="130"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ83096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="194" mass="20991" checksum="E8B60E863164E2AC" modified="2013-07-24" version="1">MSTVITIIIAAVLTNNFVLSKFYGCCPFLGVSKKLDSSIGMGAAVVFVMVCAALVTYPIYYFLLEPNDLTYLRTVAFILVIAVFVQLLEMIIKKVMPPLYKTLGVYLPLITTNCAVLGVTIMNIDENYTFVQSLINAFFSGFGFALAMVLFSGVRSRVDDADEETPAMFRGVPATLVAAAIVSMSFLGFGGLGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5VI56</accession>
    <name>R5VI56_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">L-serine dehydratase</fullName>
            <ecNumber evidence="1">4.3.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01022</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-serine = NH4(+) + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:19169"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="EC" id="4.3.1.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the iron-sulfur dependent L-serine dehydratase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.17" evidence="1"/>
    <dbReference type="EMBL" id="CBAR010000081">
        <property type="protein sequence ID" value="CCZ83241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01022"/>
    <dbReference type="EnsemblBacteria" id="CCZ83241">
        <property type="protein sequence ID" value="CCZ83241"/>
        <property type="gene ID" value="BN566_01022"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003941">
        <property type="term" value="F:L-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005130">
        <property type="entry name" value="Ser_deHydtase-like_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004642">
        <property type="entry name" value="Ser_deHydtase_asu"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03313">
        <property type="entry name" value="SDH_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00718">
        <property type="entry name" value="sda_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="1" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SDH_alpha" evidence="2">
        <location>
            <begin position="17"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU366059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="295" mass="30743" checksum="4DA68D23FB73C9B9" modified="2013-07-24" version="1">MGFSSVAAWVAACQETQLPMWEVILRQDLDQDTPEARAASLKQMKHMWEVMKNSASQYQSEQRSYSGLVGGDGARYAEAWASGILMGDCYWNAAIATALKVSECNACMKRIVAAPTAGSCGVIPAALIPYLECYPDTTEEDMVHALYLAGGFGNIIAERASISGAEAGCQAEIGAASAMAAAALTFLRGGTPEACGNACAIALKNLLGLVCDPVAGLVEIPCVKRNVIGVVNAISSANLAMAGITSRIPADEVIDAMGEIGRHMPESVRETAQGGLAATPTGQEIAETVLKRPAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5VIA2</accession>
    <name>R5VIA2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">N-formylglutamate deformylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01072</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01072"/>
    <dbReference type="EnsemblBacteria" id="CCZ83291">
        <property type="protein sequence ID" value="CCZ83291"/>
        <property type="gene ID" value="BN566_01072"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007709">
        <property type="entry name" value="N-FG_amidohydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05013">
        <property type="entry name" value="FGase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="265" mass="30413" checksum="E9008B5A2E8B832E" modified="2013-07-24" version="1">MIQETNLKPYTIYHSENHSFPLVVSIPHSGTQLTREMQETLLPDVVLSNMDWYLPQLYAFLVEWGVTVLVNPISRYVIDPNRPPFQKIGDAYTTNCIYETTTSGEPMYAAPLTEVVRLERKRCYYDAYHQALKQLLAKKKQRFSKVYFLDLHSYGMDTGSDAIIGTQFGQTCSAAYSTTLQTLLEQEGLRVAENQPFAGGYLTKHYGADTQIEAAQIELSYAYYIDHRTFGREVFPEICPAIFADAQQHLQLFFKNLLKEQMICQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>R5VIC0</accession>
    <name>R5VIC0_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">Cysteine desulfurase</fullName>
            <ecNumber evidence="4 6">2.8.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_01092</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the removal of elemental sulfur and selenium atoms from L-cysteine, L-cystine, L-selenocysteine, and L-selenocystine to produce L-alanine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>[sulfur carrier]-H + L-cysteine = [sulfur carrier]-SH + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:43892"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="EC" id="2.8.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 5">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. Csd subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.7" evidence="4 6"/>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01092"/>
    <dbReference type="EnsemblBacteria" id="CCZ83311">
        <property type="protein sequence ID" value="CCZ83311"/>
        <property type="gene ID" value="BN566_01092"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031071">
        <property type="term" value="F:cysteine desulfurase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006534">
        <property type="term" value="P:cysteine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06453">
        <property type="entry name" value="SufS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020578">
        <property type="entry name" value="Aminotrans_V_PyrdxlP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010970">
        <property type="entry name" value="Cys_dSase_SufS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016454">
        <property type="entry name" value="Cysteine_dSase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005572">
        <property type="entry name" value="NifS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01979">
        <property type="entry name" value="sufS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00595">
        <property type="entry name" value="AA_TRANSFER_CLASS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_5" evidence="7">
        <location>
            <begin position="22"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="408" mass="44915" checksum="10328A97FF0FBD5C" modified="2013-07-24" version="1">MTASQIAAIRAEFPLIAKHDRVYFDNAATSQKPACVLQAAQEFYESYNANVLRGLYPLSVEATERYEAARTTVQQFIHAAAPEEIIFTRNTTESINLVAYSYGLSHLKADDEIVVSILEHHSNLLPWQMVARMTGAKLVYLECDPDGTISKERMDAAFSDHTKLVAIGMVSNVLGCVNPVAELVKRARQCGAVILMDAAQGAPHMAVDVQALDVDFMAFSGHKMLAPMGIGVLYGKKELLESMPPFLTGGEMIETVSRYDATYAELPHKFEAGTVNAGGAVALAEAIRYLQKLGMSNVQQAEQELTEYLLRGMQSIPHIHVLGSEQAENHTGIVAFTVDQVHPHDISEILSSDGMDIRAGHHCAEPLHQYLGIHSSARASVMFYNTKEETDRFLESVSNIRRRMGYGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VID5</accession>
    <name>R5VID5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01107</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000086">
        <property type="protein sequence ID" value="CCZ83326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83326">
        <property type="protein sequence ID" value="CCZ83326"/>
        <property type="gene ID" value="BN566_01107"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="57" mass="6690" checksum="E99943ECE856BCEC" modified="2013-07-24" version="1">MREITELQIARKQLAEQQYYYHDCKTAETKHADTAEQPLLDLAADVLIQILRKESEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5VIG5</accession>
    <name>R5VIG5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methylase involved in ubiquinone/menaquinone biosynthesis</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01127</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000092">
        <property type="protein sequence ID" value="CCZ83351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01127"/>
    <dbReference type="EnsemblBacteria" id="CCZ83351">
        <property type="protein sequence ID" value="CCZ83351"/>
        <property type="gene ID" value="BN566_01127"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="Methyltransf_11" evidence="1">
        <location>
            <begin position="51"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="204" mass="23046" checksum="A3F1DF4AEACFF4A7" modified="2013-07-24" version="1">MSKIGDYFASQCGNPHGVIGKIMTWAMNRANNVMYKGIVDELKISPKTRILDVGFGNGYLETLIIQKSRCFITGIDISEDMVSKAAAANRRYVASGNMKFQLGDCCDLSFKDKSFDVVTTMNTIYFWSDTAKGMAEISRVLKDGGIFYNAVISKENLDKYFYTKNGFKKFTKDEYIELGKKAGFQRIRIKILGNNYGLLIVYMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5VIH9</accession>
    <name>R5VIH9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01137</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000093">
        <property type="protein sequence ID" value="CCZ83361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83361">
        <property type="protein sequence ID" value="CCZ83361"/>
        <property type="gene ID" value="BN566_01137"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="84"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="114" mass="13689" checksum="6E83B80BE0E44D69" modified="2013-07-24" version="1">MKPFDTDKMPWLFTPHGEGELNEYFSSDHSIVISETSKEVIASLSESRDRFSGKLCYIFNYNQRRYRYTLLRFADVIVRADVSIKMLKKRIMELDIDNRELRKQLADMERKMKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5VIT5</accession>
    <name>R5VIT5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">S4 domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01210</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000104">
        <property type="protein sequence ID" value="CCZ83436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01210"/>
    <dbReference type="EnsemblBacteria" id="CCZ83436">
        <property type="protein sequence ID" value="CCZ83436"/>
        <property type="gene ID" value="BN566_01210"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="77" mass="8397" checksum="CD5C22359C34609F" modified="2013-07-24" version="1">MEKEHLTVTIHTPFIKLDALLKFAGLCDTGGFAKELVQQGVVSVNGSVCTMRGKKIYPNDVITVDHFEVQVTGDSCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJ37</accession>
    <name>R5VJ37_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">RNA polymerase sigma factor sigma-70 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_01265</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000118">
        <property type="protein sequence ID" value="CCZ83496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83496">
        <property type="protein sequence ID" value="CCZ83496"/>
        <property type="gene ID" value="BN566_01265"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="3">
        <location>
            <begin position="24"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="4">
        <location>
            <begin position="124"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="186" mass="21169" checksum="5E6544789A811F88" modified="2013-07-24" version="1">MQEETLLDLYFSRSETALEQTKQQYGTYCYAIAYRILAQPQDAEECENETYWKAWQAIPPNRPHSLKAFLGKITRNLALHFWERNHAAKRGGNAVDLALDELTECIPASDSVEQAADAAALQACLQSFLQQLPRATRIVFVQRYWYLNSIAEIAQKNGMGESKVKMLLLRTRKKLKAALETEGISI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJ92</accession>
    <name>R5VJ92_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transketolase C-terminal subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01344</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000131">
        <property type="protein sequence ID" value="CCZ83576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01344"/>
    <dbReference type="EnsemblBacteria" id="CCZ83576">
        <property type="protein sequence ID" value="CCZ83576"/>
        <property type="gene ID" value="BN566_01344"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transket_pyr" evidence="1">
        <location>
            <begin position="8"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="317" mass="33396" checksum="7E263A0C8D85F9B8" modified="2013-07-24" version="1">MAGETKKKATRESYGEALRDFAETYENLVVLDADLAAATKTGIFKKAYPERFFDCGIAEANMMGVAAGLAASGKIPFASTFAMFAAGRAYEIVRNSIGYPHLNVKIGATHAGISVGEDGATHQCNEDIALMRTIPGMTILNPADDVEARAAVKAAIEMEGPVYLRFGRLAAPIFNDPETYQFEIGKGITLREGKDVTLVATGLMVSEALDAAEQLAAEGISARVLNIHTIKPLDVDLIAKAAAETGLLITVEEHSIIGGLGAAVAEAVCDTCPVPVVRIGVNDEYGHSGPAVDLLKEFGLCAEHIVEVTKASMAKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJB2</accession>
    <name>R5VJB2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01369</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000144">
        <property type="protein sequence ID" value="CCZ83606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83606">
        <property type="protein sequence ID" value="CCZ83606"/>
        <property type="gene ID" value="BN566_01369"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015315">
        <property type="entry name" value="DUF1963"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035948">
        <property type="entry name" value="YwqG-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09234">
        <property type="entry name" value="DUF1963"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103032">
        <property type="entry name" value="SSF103032"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="289" mass="33662" checksum="FDC6E70D7911448C" modified="2013-07-24" version="1">MQENKFEMKDIVPELLQRTEIPCMRLKLVDTTPGIAESKFGGRGYVPHDAAIPTNSEGVQMRLLAQIDCRQITIPDFPTSGLLQFWAINDDLTGCDFDNNTEQKDFRICYYETVDPTVTEEEVAAKEGNNLPCEEDFFPIEGCFGLEFEQTTDRMSMSDYRFWEKIEQLMREFYPEQAEQLLADEDAKEAFEEELEEYLWDQDELDETPDWLPDAPGHKIGGYPCFTQEDPRNIPKEDHHDILLLQIDTDNIGEREIMWGDSGVANFFISRENLKQRNFQDVIYTWDCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJJ0</accession>
    <name>R5VJJ0_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Aminotransferase</fullName>
            <ecNumber evidence="2">2.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01437</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CBAR010000154">
        <property type="protein sequence ID" value="CCZ83701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01437"/>
    <dbReference type="EnsemblBacteria" id="CCZ83701">
        <property type="protein sequence ID" value="CCZ83701"/>
        <property type="gene ID" value="BN566_01437"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="1" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="3">
        <location>
            <begin position="32"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="394" mass="44278" checksum="15A0A75F58E2994E" modified="2013-07-24" version="1">MINYDSLLSDQVKRMQPSGIRKFFDVAAMMEGVISLGVGEPDFQTPWVVRKEAVDILERKRIIYSANSGMMNLRKEIANYLNRKYDLTYDPDSEVIATVGGSEAIDLAIRAIVNPGDEVLVVEPSFVCYKPIVELMHGVAVPIPTKEENKFKLTPEELQAHITEKTKMLILPYPNNPTGGIMTREDLEKIAAVLRGTNILVLSDEIYSELTYGTKHCSIASIPDMWERTIYVSGFSKAFAMTGWRLGYLCAPKPLAKQMLKIHQYAIMCAPTVSQYAAIVALQECDDEVRKMVDEYDARRRIVVDGFNKMGLHCFNPEGAFYVFPCIRSTGMDSETFCEKLLQEEKVAVVPGSAFGESGEGFVRVSYAYSLKHLMEALEHIAAFVQRHKQEDTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJK0</accession>
    <name>R5VJK0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">RNA methyltransferase RsmD family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01452</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000155">
        <property type="protein sequence ID" value="CCZ83716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01452"/>
    <dbReference type="EnsemblBacteria" id="CCZ83716">
        <property type="protein sequence ID" value="CCZ83716"/>
        <property type="gene ID" value="BN566_01452"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031167">
        <property type="term" value="P:rRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004398">
        <property type="entry name" value="RNA_MeTrfase_RsmD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004553">
        <property type="entry name" value="CHP00095"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00095">
        <property type="entry name" value="TIGR00095"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="186" mass="20874" checksum="DFA4851288567643" modified="2013-07-24" version="1">MRIISGTARGRRLKTLEGMDVRPTTEKVKEAIFSSIQFDLPQATVLDLFAGSGQLGLEALSRGAKRAYLIDHSPKALSVIRENVAAAGFSEQAEVIQTEAATFLKSSAPTMQWDLVFLDPPYRHDTIQEMLLLLEHHVAPDGKIICEHERELELPEELGVWRKKKTYSYGSIAVTVYIYKEEVTQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJT6</accession>
    <name>R5VJT6_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Bifunctional protein FolD</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="8">Methylenetetrahydrofolate dehydrogenase</fullName>
                <ecNumber evidence="8">1.5.1.5</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="8">Methenyltetrahydrofolate cyclohydrolase</fullName>
                <ecNumber evidence="8">3.5.4.9</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="8" type="primary">folD</name>
        <name evidence="11" type="ORF">BN566_01541</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NADP(+) = 5,10-methenyltetrahydrofolate + NADPH</text>
            <dbReference type="Rhea" id="RHEA:22812"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.5.1.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>5,10-methenyltetrahydrofolate + H2O = (6S)-10-formyltetrahydrofolate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57454"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="EC" id="3.5.4.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.5" evidence="8"/>
    <dbReference type="EC" id="3.5.4.9" evidence="8"/>
    <dbReference type="EMBL" id="CBAR010000164">
        <property type="protein sequence ID" value="CCZ83806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01541"/>
    <dbReference type="EnsemblBacteria" id="CCZ83806">
        <property type="protein sequence ID" value="CCZ83806"/>
        <property type="gene ID" value="BN566_01541"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004477">
        <property type="term" value="F:methenyltetrahydrofolate cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004488">
        <property type="term" value="F:methylenetetrahydrofolate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01080">
        <property type="entry name" value="NAD_bind_m-THF_DH_Cyclohyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01576">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminiacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000672">
        <property type="entry name" value="THF_DH/CycHdrlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020630">
        <property type="entry name" value="THF_DH/CycHdrlase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020867">
        <property type="entry name" value="THF_DH/CycHdrlase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020631">
        <property type="entry name" value="THF_DH/CycHdrlase_NAD-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00763">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02882">
        <property type="entry name" value="THF_DHG_CYH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00085">
        <property type="entry name" value="THFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="SSF53223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00766">
        <property type="entry name" value="THF_DHG_CYH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00767">
        <property type="entry name" value="THF_DHG_CYH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="4 8" id="KW-0521">NADP</keyword>
    <keyword evidence="1 8" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="THF_DHG_CYH" evidence="9">
        <location>
            <begin position="5"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="THF_DHG_CYH_C" evidence="10">
        <location>
            <begin position="123"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="165"/>
            <end position="167"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ83806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="285" mass="30800" checksum="9699475341570EDF" modified="2013-07-24" version="1">MAKLISGKIVSVKVRNEVKKETEELRENHGIVPGLAVILVGNDPASQVYVRNKQRACEEVGFHPYEYRMNENATQEQLLDLIGVLNKDNKVNGILVQLPLPEHIDEQAVIHAISPLKDVDAFHPVNVGKIMIGDYDFLPCTPAGIMELIASTGVDISGKQCVVIGRSNIVGKPMAMLLLQKNGTVTICHSRTKNLKELAASADILVVAIGKPKFVTADMVKEGAIVIDVGINRMEDGKLCGDVDFDSVEPKAGYITPVPGGVGPMTIAMLMKNTLHAAKLQHNLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJZ6</accession>
    <name>R5VJZ6_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Flavin-dependent thymidylate synthase</fullName>
            <shortName evidence="1">FDTS</shortName>
            <ecNumber evidence="1">2.1.1.148</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">FAD-dependent thymidylate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Thymidylate synthase ThyX</fullName>
            <shortName evidence="1">TS</shortName>
            <shortName evidence="1">TSase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">thyX</name>
        <name evidence="2" type="ORF">BN566_00171</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor, and NADPH and FADH(2) as the reductant.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP + H(+) + NADPH = (6S)-5,6,7,8-tetrahydrofolate + dTMP + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:29043"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <dbReference type="EC" id="2.1.1.148"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="1">Binds 4 FAD per tetramer. Each FAD binding site is formed by three monomers.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; dTTP biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the thymidylate synthase ThyX family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.148" evidence="1"/>
    <dbReference type="EMBL" id="CBAR010000168">
        <property type="protein sequence ID" value="CCZ83866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00171"/>
    <dbReference type="EnsemblBacteria" id="CCZ83866">
        <property type="protein sequence ID" value="CCZ83866"/>
        <property type="gene ID" value="BN566_00171"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00575"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050797">
        <property type="term" value="F:thymidylate synthase (FAD) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006231">
        <property type="term" value="P:dTMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01408">
        <property type="entry name" value="ThyX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003669">
        <property type="entry name" value="Thymidylate_synthase_ThyX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036098">
        <property type="entry name" value="Thymidylate_synthase_ThyX_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34934">
        <property type="entry name" value="PTHR34934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02511">
        <property type="entry name" value="Thy1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69796">
        <property type="entry name" value="SSF69796"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02170">
        <property type="entry name" value="thyX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51331">
        <property type="entry name" value="THYX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="1" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Involved in ionization of N3 of dUMP, leading to its activation" evidence="1">
        <location>
            <position position="192"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <note>ligand shared between neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="76"/>
            <end position="79"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="79"/>
            <end position="81"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="89"/>
            <end position="91"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="181"/>
            <end position="183"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <note>ligand shared between neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="262" mass="28935" checksum="C7E753208D43CAB1" modified="2013-07-24" version="1">MTVTLISHTPQPEQIIAAAAKLCYSDTTVEDLMDGLSAEQAEKFVGMLETMGHESPVEHVTFTFGIAGVSRSLLAQITRHRIASFSVQSQRYVRKNQFTYVTPPAIAADEAAAALYHQTMEQTVQAYNALADRLQATYYPQFLAEGCTEKAARSKAEKKAIEDARYVLPNACETKMMVTMNVRSLRHFFQLRCCNRAQWEIRDLATQMLKLCCEAAPALFRHAGPACCQGGCPEGKMTCGQMAAVRERFAALHAAPSTAETR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5VKB9</accession>
    <name>R5VKB9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative stage III sporulation protein AD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01695</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000177">
        <property type="protein sequence ID" value="CCZ83966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01695"/>
    <dbReference type="EnsemblBacteria" id="CCZ83966">
        <property type="protein sequence ID" value="CCZ83966"/>
        <property type="gene ID" value="BN566_01695"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025664">
        <property type="entry name" value="Spore_III_AC/AD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06686">
        <property type="entry name" value="SpoIIIAC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="130" mass="14109" checksum="F1B829BCD4FAEA30" modified="2013-07-24" version="1">MIAETGMTAAVCIIGAVLACLLKQYCKEQALFCSIGVCVLVLLSICAYLSPIAEQIQALFLKTELDVQYLKLLWKSIGICYLTSIAADLCRDTGETAMASVAELWGRVTLLILFLPMAKSLLELMTGVLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5VKL7</accession>
    <name>R5VKL7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01762</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the EamA transporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000181">
        <property type="protein sequence ID" value="CCZ84036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01762"/>
    <dbReference type="EnsemblBacteria" id="CCZ84036">
        <property type="protein sequence ID" value="CCZ84036"/>
        <property type="gene ID" value="BN566_01762"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="44"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="108"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="196"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="224"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="258"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="283"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="3">
        <location>
            <begin position="16"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="3">
        <location>
            <begin position="168"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="308" mass="33191" checksum="D34275C72082CCC6" modified="2013-07-24" version="1">MQKSFLQKTWVVAGVAFLCSALWGSAFAGVKIGYELFQIASADWAAQMVFAGIRFAIAGVMALVGGSIVAKQALIPQKQTIPKISIISLFQTILQYFFYYIGLAHTTGVNAAIIVAANVFIAILLSTLVYRIEKMTAAKLFGCVIGFLGIILCNLNGLKGGIHLSFLGDGFIFLCTVASGFSSVLMKKYAASENPILLSGWQFLFGGVVMFAVGLLFGGRMEHITGSAIAILLYLSFVSAAAYSLWSLLLKYNPVSKVAVFGFLNPICGVCISTIFLKEKDAFHIFTIPALIFVCVGIYIVNHKKQNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5VKL9</accession>
    <name>R5VKL9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01767</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000181">
        <property type="protein sequence ID" value="CCZ84041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01767"/>
    <dbReference type="EnsemblBacteria" id="CCZ84041">
        <property type="protein sequence ID" value="CCZ84041"/>
        <property type="gene ID" value="BN566_01767"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="220" mass="24811" checksum="95B90FA9F30922ED" modified="2013-07-24" version="1">MINGVIFDMDGTLLDTERVLIRCWIKSAALHGFEMKEQHALNIRSLQYRFAEPYLKRVFGESFDYMAIRETRKQLFAEELQKNGIPVKQGVLPFLQFCKEQQISTVVATATPKDRATRYLEEVGFLPYFTEIFGSNQVKTGKPFPDIYQAAAQSINCAPSDCMAFEDAPNGVLSAFYAGCKVVMIPDLSQPDEMLQPFLYAVAPNLEQAIPILKEAIQHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>R5VKT4</accession>
    <name>R5VKT4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">33 kDa chaperonin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Heat shock protein 33 homolog</fullName>
            <shortName evidence="6">HSP33</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">hslO</name>
        <name evidence="7" type="ORF">BN566_01856</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Redox regulated molecular chaperone. Protects both thermally unfolding and oxidatively damaged proteins from irreversible aggregation. Plays an important role in the bacterial defense system toward oxidative stress.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="6">Under oxidizing conditions two disulfide bonds are formed involving the reactive cysteines. Under reducing conditions zinc is bound to the reactive cysteines and the protein is inactive.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the HSP33 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000191">
        <property type="protein sequence ID" value="CCZ84131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01856"/>
    <dbReference type="EnsemblBacteria" id="CCZ84131">
        <property type="protein sequence ID" value="CCZ84131"/>
        <property type="gene ID" value="BN566_01856"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00498">
        <property type="entry name" value="Hsp33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.30.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00117">
        <property type="entry name" value="HslO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000397">
        <property type="entry name" value="Heat_shock_Hsp33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016154">
        <property type="entry name" value="Heat_shock_Hsp33_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016153">
        <property type="entry name" value="Heat_shock_Hsp33_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30111">
        <property type="entry name" value="PTHR30111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01430">
        <property type="entry name" value="HSP33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005261">
        <property type="entry name" value="Heat_shock_Hsp33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118352">
        <property type="entry name" value="SSF118352"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64397">
        <property type="entry name" value="SSF64397"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 6" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0862">Zinc</keyword>
    <feature type="disulfide bond" description="Redox-active" evidence="6">
        <location>
            <begin position="237"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="6">
        <location>
            <begin position="270"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ84131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="291" mass="30997" checksum="082A700640CD2ECC" modified="2013-07-24" version="1">MAILKRAISKDASVVSYALDATDMVAEIERIHETSAVVTAALGRLAIAASMMGYGLKGEKDTVTLRVDGKGPAGQLVAVADSHGNVKCDVEHPIVEIPLNAHGKLDVGGAVGTDGMLSVVKDLGLKEPYVGMCPLVSGEIAEDIASYFATSEQTPTVCGLGVLVNPDLHVKAAGGYLIQVLPFASDACIDQLEKNIQGLPHVTKLLTDGMTPDQIALRLLDGLEPNLLDEATVSYHCDCSQERTERVLVSLGKQELEQMAADGEDITVTCHFCHKQYTFTPDELRKLAKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>R5VL20</accession>
    <name>R5VL20_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dockerin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01961</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01961"/>
    <dbReference type="EnsemblBacteria" id="CCZ84236">
        <property type="protein sequence ID" value="CCZ84236"/>
        <property type="gene ID" value="BN566_01961"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002105">
        <property type="entry name" value="Dockerin_1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027849">
        <property type="entry name" value="DUF4434"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033402">
        <property type="entry name" value="DUF5109"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026906">
        <property type="entry name" value="LRR_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00404">
        <property type="entry name" value="Dockerin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14488">
        <property type="entry name" value="DUF4434"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17134">
        <property type="entry name" value="DUF5109"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13306">
        <property type="entry name" value="LRR_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004395433" description="Dockerin domain-containing protein" evidence="2">
        <location>
            <begin position="30"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="3">
        <location>
            <begin position="690"/>
            <end position="753"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="753" mass="85432" checksum="9B917A42FC2F3CB6" modified="2013-07-24" version="1">MKNHFWITIASCLSLFLTLFIPSNIHAQAESSHIEKIDGTFIQPWLYMTYDEEQWDNEMQALKEVGIDYLIMGDVANHNSDGTWTVYYPSKLDYLSDYIAYDALEPLLYYCEKYDIKLYLGMGLDCAWNSDIVSEAGRKANQTYMKQCNEITTELYNQYKSRYPNTYYGFYFVTELYNTLYMDTDTGIDAYADSLDEMFTMVIENCNALDESMPLLFSPYVNIFGYGYASVNIDRFTEYWSDVLSRIPFRNGDMICPQDSCGGGGMDVAHLAEWTKAYRNAVDRSNKNRGTTLLLGTNAEMFVQPDAERMTAPHGVSYSGIKNVRDFTTRLETAKPFVNALFCFAYPHHYSPYNTDPQFHECLKTYLKTGEIESEPPTPPTNIEVQLIESNGKQLPRFTFTGMKDNTSVVHTNIYKNGKLYDYIVPAAKVGGNATQNIWTDYNFTDDNAIYEIECVDVCGNVSDQCSFSLDWNALKETGSLSITPSDAIDWNWTSLDFLSYSVTDSGVRINGCDKNAVHIVIPDEIEGKPVTVIDWYAFERCEKLESVVIPETVTHISRFAFAHCISLESVNLPKSLYSIEQYAFYDCPNLKGLNLPENLNIIEDRAFSGCRSISKIVIPKSCQNIGEYAFLDCDSLNTLTLNGEHTTLGTKSFGYEYDNGYAVKDSFLLISQNENAIRYAEENHIQILSEIISGDGNADGVCNLADAVLLQKWLLDMPETKLINWKAVDCNEDNQLDIIDLCLLKKKLLAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VLD1</accession>
    <name>R5VLD1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00037</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000219">
        <property type="protein sequence ID" value="CCZ84356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84356">
        <property type="protein sequence ID" value="CCZ84356"/>
        <property type="gene ID" value="BN566_00037"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="36" mass="4131" checksum="AD6C88495C0DF644" modified="2013-07-24" version="1">MGTRNAEERRKAAGKHRKESFSVKLEVVAKYEPQAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5VLN5</accession>
    <name>R5VLN5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00355</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000234">
        <property type="protein sequence ID" value="CCZ84456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00355"/>
    <dbReference type="EnsemblBacteria" id="CCZ84456">
        <property type="protein sequence ID" value="CCZ84456"/>
        <property type="gene ID" value="BN566_00355"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025584">
        <property type="entry name" value="Cthe_2159"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14262">
        <property type="entry name" value="Cthe_2159"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="807"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="985"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="985"/>
            <end position="999"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="1005" mass="101464" checksum="DE88F39915AB1B3C" modified="2013-07-24" version="1">MQQYLLQERTLSAEAGANADYNADGVCNVIDLAMIKRTVLESNPVTNTIAIHLSDSGITVENDTKGVTSVSGKTVTISASGSYTVDGSMADGQILVNIPDTTADADDVELILNNVTMTSSTGAPCIYTQSAVKTKLTVTGTNTFTDTATAANVDTSGVIYADGKLTVTKNSTGTLQVTSLMNVGLYATKNINLNGGTIQVDTDVDSTSDADAIKTKKTLTVEGATVTVDASADGLKSTKELVNIVSGNVSVKAGNDAIQAATDINISGGIVVAGGDRGFRLDDGGKLNITGGDVLATATDYQITNSTDSLATVDLSGCTQGVMMLDFAAEQAKKQPITVQQNGNTVYEMTTNKKCSYALLSGSGLTASGSYTTAVNSAEQGHTPSGSTTQESTFVMSGTATEFYQVAATGMVVTPTDDTAVATISYSNSGVQLLNASGKDVSTSNSSGITVDGSTVTLTQPAMVDVTGESSNARIVVNVDKTTYANGVVELCLKNATLSNATTAPIYVEAIGDEVVLNSSNGTVNTISDGTSHTDTYVDSDGVTNTVSGAVFSRDDMKLKGSGSLTINGNTEDGLVCKNDLKLYNGTITVTAKDDAIRGNDSITIGNASATDYKNLNVTATSTAGDAFKTKDTDTTSGKGYLTINGGTVQATAYADAFHSSQTLTVNGGDINIQTTCPASNSNTGGWGGFPGGGGDGNSDQTDISAKGLKAGCTDDNNNTIAGNITIAGGTITIDSTDDSVHATNITMTGGTVTAATGDDGMHADNKLDIQAGTVIITKSYEGLEAVDLQINGGNIHVNASDDGLNAAGGNDSSGNNGGWGGGGWGGGMSSSTGTLTINDGYLVVRAEGDGLDSNGDLIINGGTVLVYGPTTGGNGIFDKGDGNYSFQINGGTVWGCGTSDMFESPNKTYLSGTVSATAGATFAAADASGNVSSIMTVPSDMNMSNAMLFYCGSDVSSVGLYSGGTYSGGTMNADGYATGGTLSGGSSVSSSSSGGGNNRPGGRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>R5VLQ9</accession>
    <name>R5VLQ9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phosphate transport system permease protein PstA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_00375</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000238">
        <property type="protein sequence ID" value="CCZ84476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00375"/>
    <dbReference type="EnsemblBacteria" id="CCZ84476">
        <property type="protein sequence ID" value="CCZ84476"/>
        <property type="gene ID" value="BN566_00375"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:inorganic phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005672">
        <property type="entry name" value="Phosphate_PstA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00974">
        <property type="entry name" value="3a0107s02c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="25"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="117"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="199"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="263"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="7">
        <location>
            <begin position="79"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ84476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="292" mass="31620" checksum="D1D3ED729F441E36" modified="2013-07-24" version="1">MAAPKVGINQRSFRQKLAEYRHQPLSLFLLIATLLAAAITVFILFSLILYILVKGIPAIRPEMFAWKYTTSNNSMMPSIINTLEMVVLTLIMAVPVGVCSAIYLVEYAKRGNKLVKVVRMTTETLQGIPSIVFGLFGYLMFVVACHFGYSLLGGAITLALMVLPLIMRTTEEALMSVPDSFREGSFGLGAGRLRTVFKIIVPSAMPGILSGVILAIGRIVGETAALMYTAGTATSVCVSPMGTCRTLAVHMYQLLCEGREMNAAYATAVVLLVMVILINLLSSAIAKKLTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5VQ90</accession>
    <name>R5VQ90_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00002</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000001">
        <property type="protein sequence ID" value="CCZ82747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82747">
        <property type="protein sequence ID" value="CCZ82747"/>
        <property type="gene ID" value="BN566_00002"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009702">
        <property type="entry name" value="DUF1284"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06935">
        <property type="entry name" value="DUF1284"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ82747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="141" mass="16016" checksum="E146D7A3DAA56EF3" modified="2013-07-24" version="1">MQPYKLRPHHGLCSSFFCGHGYSADFTEHMTKTLLHFQQHNPQILLTVGADCICAACPHCCHDCCSSAEQVERYDRAVLNSCGLREGDVLDWNAFRRQVQEQILHTEVFPHICGTCSWYSICSRKEATAISQAFLQKQILP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>R5VQH4</accession>
    <name>R5VQH4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosome-binding ATPase YchF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ychF</name>
        <name evidence="8" type="ORF">BN566_00642</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">ATPase that binds to both the 70S ribosome and the 50S ribosomal subunit in a nucleotide-independent manner.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family. YchF/OLA1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000012">
        <property type="protein sequence ID" value="CCZ82837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00642"/>
    <dbReference type="EnsemblBacteria" id="CCZ82837">
        <property type="protein sequence ID" value="CCZ82837"/>
        <property type="gene ID" value="BN566_00642"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04867">
        <property type="entry name" value="TGS_YchF_OLA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01900">
        <property type="entry name" value="YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00944">
        <property type="entry name" value="YchF_OLA1_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004396">
        <property type="entry name" value="ATPase_YchF/OLA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023192">
        <property type="entry name" value="TGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013029">
        <property type="entry name" value="YchF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041706">
        <property type="entry name" value="YchF_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06071">
        <property type="entry name" value="YchF-GTPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006641">
        <property type="entry name" value="CHP00092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="SSF81271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00092">
        <property type="entry name" value="TIGR00092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004395602" description="Ribosome-binding ATPase YchF" evidence="5">
        <location>
            <begin position="26"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="OBG-type G" evidence="6">
        <location>
            <begin position="1"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="7">
        <location>
            <begin position="279"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="10"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ82837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="365" mass="40314" checksum="6C04246E7E6DED13" modified="2013-07-24" version="1">MKLGMVGLPNVGKSTLFNALTNAGAESANYPFCTIEKNVGIVSVPDSRLDELAKIYEPDKFTPATLEFVDIAGLVKGASKGEGLGNKFLGDIREVDAIVHVVRCFEDVNIVHVDGSINPARDIETINLELIFADLDMLNRRIDRVKKALKGDKKLQNQLDFLEQLKAHLEEGKSARAFDMTEEEQEWLKETPLLSAKPVIYAANLCEKDFSGDLAEHPHYQTVCKIAAEEHAAVLPICAKIEAEISDLEDDEKEMFLSEMGLEESGLNRIIREGYSLLGLISYLTAGKPEVRAWTIRKGTKAPQAAGKIHTDFEKGFIRAEVVSFDDFMACGGSMAAAKEKGVVRLEGKDYVMQDGDIVLFRFNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>R5VQJ9</accession>
    <name>R5VQJ9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 14">Formate acetyltransferase</fullName>
            <ecNumber evidence="5 14">2.3.1.54</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 14">Pyruvate formate-lyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">BN566_00662</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>acetyl-CoA + formate = CoA + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:11844"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.1.54"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 14">Fermentation; pyruvate fermentation; formate from pyruvate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the glycyl radical enzyme (GRE) family. PFL subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.54" evidence="5 14"/>
    <dbReference type="EMBL" id="CBAR010000012">
        <property type="protein sequence ID" value="CCZ82857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00662"/>
    <dbReference type="EnsemblBacteria" id="CCZ82857">
        <property type="protein sequence ID" value="CCZ82857"/>
        <property type="gene ID" value="BN566_00662"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00920">
        <property type="reaction ID" value="UER00891"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008861">
        <property type="term" value="F:formate C-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01678">
        <property type="entry name" value="PFL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005949">
        <property type="entry name" value="Form_AcTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001150">
        <property type="entry name" value="Gly_radical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004184">
        <property type="entry name" value="PFL_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01228">
        <property type="entry name" value="Gly_radical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02901">
        <property type="entry name" value="PFL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01255">
        <property type="entry name" value="pyr_form_ly_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51149">
        <property type="entry name" value="GLY_RADICAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51554">
        <property type="entry name" value="PFL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="14" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 14" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="10 14" id="KW-0556">Organic radical</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PFL" evidence="18">
        <location>
            <begin position="3"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="domain" description="Glycine radical" evidence="17">
        <location>
            <begin position="629"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="613"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="206"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU368075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CCZ82857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="694" mass="78158" checksum="8C867840A31350C6" modified="2013-07-24" version="1">MAETMQKEWEGFVGGAWTSNVNVRDFIQKNYTPYEGDESFLVGPTATTTELWNEVLNLFQKERENGGVLDMDTKIVSTVNSHEAGYLDKDKETIVGFQTDKPLKRSLQPFGGIRMAEEACKAYGYEVDPEISYIFKHYRKTHNEGVFDVYTPEMRSARHNAIITGLPDAYGRGRIIGDYRRVALYGVDKLIEDKEHQKAITCGTMTEEVIRLREELAEQIKALKALKAMAQRYGFDISKPATNAQEAIQWLYFGYLGAVKDQNGAAMSLGRTSTFIDIYIERDMKKGILTEEQAQEFIDHFIMKLRMVKFARTPEYNALFSGDPQWVTESIGGMGVDGRTMVTKTSFRYLHTLENLGTAPEPNLTVLWSKRLPTPFKRYCAKLSINTSSFQYENDDLMRVTHGDDYAIACCVSSMRVGKDMQFFGARANLAKCLLYAINGGKDERIDAKTGKHNQVGPKYRPVEGDYLDYDDVMDKYEDMMRWLANLYVNTLNCIHYMHDKYSYEALQMALHDRDVKRYFATGIAGMSVVADSLSAIKYAKVKCIRDEFGVVTDFEIEGDFPKYGNNDDRVDSIAVEILDKFMDMIRSNYCYRNGVPTTSILTITSNVVYGKKTGNTPDGRKKGQPLAPGANPMHGRDTHGAAASMASVAKLPWTNAQDGISNTFTIIPAALGKDSQVFAGDIDIASIRESIEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5VR51</accession>
    <name>R5VR51_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00800</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000038">
        <property type="protein sequence ID" value="CCZ83007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83007">
        <property type="protein sequence ID" value="CCZ83007"/>
        <property type="gene ID" value="BN566_00800"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="17"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="72" mass="7923" checksum="B6E12D1066D55042" modified="2013-07-24" version="1">MLKKTWEQIADTPKKEVALPFACGVACGILIGLLAVPFSKGIVIGSYNNCNHRVSDESVRELPEQDGENYEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>R5VRE6</accession>
    <name>R5VRE6_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Putative pre-16S rRNA nuclease</fullName>
            <ecNumber evidence="2">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00919</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Could be a nuclease involved in processing of the 5'-end of pre-16S rRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YqgF HJR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="2"/>
    <dbReference type="EMBL" id="CBAR010000062">
        <property type="protein sequence ID" value="CCZ83132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00919"/>
    <dbReference type="EnsemblBacteria" id="CCZ83132">
        <property type="protein sequence ID" value="CCZ83132"/>
        <property type="gene ID" value="BN566_00919"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000967">
        <property type="term" value="P:rRNA 5'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16964">
        <property type="entry name" value="YqgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00651">
        <property type="entry name" value="Nuclease_YqgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005227">
        <property type="entry name" value="YqgF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006641">
        <property type="entry name" value="YqgF/RNaseH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037027">
        <property type="entry name" value="YqgF/RNaseH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33317">
        <property type="entry name" value="PTHR33317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03652">
        <property type="entry name" value="RuvX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00732">
        <property type="entry name" value="YqgFc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00250">
        <property type="entry name" value="RNAse_H_YqgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="YqgFc" evidence="3">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="145" mass="15984" checksum="F7D14A6A1F54631C" modified="2013-07-24" version="1">MIILGVDFGEARTGLAICDRSELLASPVGTIAETDFDTCVEKVAAAAKERRAACIVVGYPKNMNGTIGERAERCQQFAACLKEKTNLPTVLWDERSTTVTAHQYLNQTNTRGKKRKAVVDTVAATIILESYLAYRKHHPEDCSEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5VRK3</accession>
    <name>R5VRK3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00970</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000069">
        <property type="protein sequence ID" value="CCZ83187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83187">
        <property type="protein sequence ID" value="CCZ83187"/>
        <property type="gene ID" value="BN566_00970"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="117"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="306"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="334" mass="38682" checksum="51C26114DA3E00EA" modified="2013-07-24" version="1">MAEEIPKQTTSEQETTAPTGKKKKEKGKSFLETMREIDAKEQQQEEAREAAMQAQLAEQEKREKEAYEKKIQQDRIELIRLKQGVITESDRIAEEHPEKKKMPLGKRISNFFYHNKWWLGITIVLVLLFGYLAYDQLTKVKPDFVLMVLTDNEELQKKSAELQTYLEQFVDDENGDGKVTVSIYCIPVSDEIDDMDYYTANATKLSTQMQMPEGVMLLTDAKANDYISAEDTLVNLEQLYPEDSHIREYGYYLRYTDFAQKIGYSEGRLDRDLCLSLRKPTEGYKSEAVMQEYYVYAKKVLEEIMTDLDNTEPPADKPVSETPETTEQTTEQGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5VRS0</accession>
    <name>R5VRS0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01038</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01038"/>
    <dbReference type="EnsemblBacteria" id="CCZ83257">
        <property type="protein sequence ID" value="CCZ83257"/>
        <property type="gene ID" value="BN566_01038"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014535">
        <property type="entry name" value="Hpre_diP_synt_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010898">
        <property type="entry name" value="Hpre_diP_synth_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07456">
        <property type="entry name" value="Hpre_diP_synt_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF027391">
        <property type="entry name" value="Hpre_diP_synt_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="184" mass="20320" checksum="6875F68D08761B8C" modified="2013-07-24" version="1">MQKSVKHLVTLAMFTTMALVIFVVEAQIPVPVPIPGIKLGLANIITLFVLQKYRVRDALAVLVLRIFLGSLFTGTLVSCLYSLSGGLLCLVGMALLCRLLQNRYLWFVSVCGAILHNIGQILAAMLVMQTTQVIWYLPFLLISGCVTGLFTGLAATYLIRHWKRTESAKQKKNETKTISAEHLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5VRX6</accession>
    <name>R5VRX6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">SufB/sufD domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01093</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01093"/>
    <dbReference type="EnsemblBacteria" id="CCZ83312">
        <property type="protein sequence ID" value="CCZ83312"/>
        <property type="gene ID" value="BN566_01093"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000825">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037284">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011542">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43575">
        <property type="entry name" value="PTHR43575"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01458">
        <property type="entry name" value="SUFBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101960">
        <property type="entry name" value="SSF101960"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="346" mass="37070" checksum="171C58D2538893ED" modified="2013-07-24" version="1">MEQQKIIVNALPVKTWHRLDVNEAKTIAYTPTAACTVQAESEQGTTQISAVSKVEPEQSVETGVGAALTPVFQWGMQIAAASNTTDTVRMTIQNAPQAEAALQVLVNVAEGSHLTIVQNWEGQANHAAVRTILHVAANATVHLIQVLADNSADSWNDVGCVAAEKGSIELTRLYLTKGNLTSGVRADLIGDGSSFTSHSGYLVQQNRTLDMNVIVNHIGKKTMSDITAEGTVQNHAAKVFRGTIDLRTGCSGSEGKENETVLLLGDDVVNKTIPLILCSEEDVKGSHGASIGELPQDILFYFASRGIEKETAEKIISRAKLETLCQGLDEKTADLLQKTIDEVIPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VS32</accession>
    <name>R5VS32_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01133</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000092">
        <property type="protein sequence ID" value="CCZ83357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83357">
        <property type="protein sequence ID" value="CCZ83357"/>
        <property type="gene ID" value="BN566_01133"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="60" mass="6961" checksum="F08CCE6D1931C7D0" modified="2013-07-24" version="1">MFCANCYNEIPAGKSYYKHQDTNQSYCSLDCLNDHMIYTHTITLETSEGEESTEEKDNDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VS52</accession>
    <name>R5VS52_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01148</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000093">
        <property type="protein sequence ID" value="CCZ83372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83372">
        <property type="protein sequence ID" value="CCZ83372"/>
        <property type="gene ID" value="BN566_01148"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="143" mass="17326" checksum="1D13A2E8219F4C04" modified="2013-07-24" version="1">MGRFMYVHFGDDVPRNIEKEYNKLLRKERYIEELDAKNGKIYPDFDDVLSSNPDPASIPISDEEEKEQIRHRNRLDYLPDALELLKSDFPEGYELIRDYFLREDKVTMWYLVEKYGLSIDMVRYRIKIAKQKLKEYIILHENE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VSA7</accession>
    <name>R5VSA7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01193</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000099">
        <property type="protein sequence ID" value="CCZ83417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83417">
        <property type="protein sequence ID" value="CCZ83417"/>
        <property type="gene ID" value="BN566_01193"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="84" mass="9212" checksum="7632ADE79BFBD640" modified="2013-07-24" version="1">MWESSFVCLLLFDSWETDCVWFGKIASLFARVCKLLLGIPPESGFGKAGAHPQLAWETAIAPETLHLITAFLFQKAIAVSAILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5VSG8</accession>
    <name>R5VSG8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative virion core protein (Lumpy skin disease virus)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00010</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000112">
        <property type="protein sequence ID" value="CCZ83472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00010"/>
    <dbReference type="EnsemblBacteria" id="CCZ83472">
        <property type="protein sequence ID" value="CCZ83472"/>
        <property type="gene ID" value="BN566_00010"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03408">
        <property type="entry name" value="SPFH_like_u1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033880">
        <property type="entry name" value="SPFH_YdjI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13421">
        <property type="entry name" value="Band_7_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="SSF117892"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Band_7_1" evidence="2">
        <location>
            <begin position="52"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="320"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="429" mass="46414" checksum="0381C6FC08B3EC5F" modified="2013-07-24" version="1">MGLIRAAVGAVGGTLSDSWLDYIRAENMTPTTILTKGEQVGKQHRRGSADIISNGSRIEVGANQMLFLTEGGKIVDYTAEQGYYEVFLSQSPSLFNGELKASVKETFERFKFGGQPGKSQRAYFVNLSEIRGIRFGTRNALQYFDNFYNAELFLRCHGTYSIKVTDPLKFYMENSSMCNTGRVDFNDINEQFNAEFLTALQTAIGKMSVDGVRISSLPSKSAELAKYLSDALDATWNELRGVEICSVGIASISYDEESKKLINMRNQGAMLSDATIREGYVQGAVAEGLKAAGSNTAGAAQAFMGMGMGMQGAGNFMAGASAANLQQMQMQQQMQAQQQQQQQQQQQQAAPANGWKCSCGATNTGKFCTECGQPKPAPVGAWKCSCGAENTGKFCTECGKPRPADGKWKCSCGAENTGKFCTECGKPRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>R5VSP3</accession>
    <name>R5VSP3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="13">D-alanyl-D-alanine carboxypeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BN566_01311</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the peptidase S11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000121">
        <property type="protein sequence ID" value="CCZ83542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01311"/>
    <dbReference type="EnsemblBacteria" id="CCZ83542">
        <property type="protein sequence ID" value="CCZ83542"/>
        <property type="gene ID" value="BN566_01311"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009002">
        <property type="term" value="F:serine-type D-Ala-D-Ala carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015956">
        <property type="entry name" value="Peniciliin-bd_prot_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018044">
        <property type="entry name" value="Peptidase_S11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001967">
        <property type="entry name" value="Peptidase_S11_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00768">
        <property type="entry name" value="Peptidase_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00725">
        <property type="entry name" value="DADACBPTASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69189">
        <property type="entry name" value="SSF69189"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="4" id="KW-0133">Cell shape</keyword>
    <keyword evidence="6" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="13" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004407298" evidence="11">
        <location>
            <begin position="31"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S11" evidence="12">
        <location>
            <begin position="29"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="357"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="7">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="68"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="120"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR618044-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR618044-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CCZ83542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="399" mass="43499" checksum="2F296AF28983FD8E" modified="2013-07-24" version="1">MCKRLFGLLLSIISTVSILCGSVWQCSATAVELPAVSAKACIVMEATTREVFFSQNADEHLPMASTTKIMTTLLCLESGDLDTPFPVDNNAIQVEGSSMGLVKDDIVTKRALCYGMLLPSGNDAAGATAVKLAGSLSAFADLMNERAAQLGMQNTHFVTPSGLHDEQHYSTAYDMAILTATALQNPDFREICGQATAKVTFGNPPYDRWLKNSNKLLTQCEGVIGVKTGFTDEAGRCLVSACQRNDMTLICVTLNDPNDWQTHTQLYDTLFPQLHKQTVALPETISIPVVGGTADTVSLQATDSITYGTLQEAVDLPLTVHTKPFLFAPVPANQYGGFLVAERDGQSLFQLPLTTQTAVPEQPATPEEQPQKSIWXXPEDQPQKSIWQRLQRWLQVRFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>R5VSX8</accession>
    <name>R5VSX8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Site-specific recombinase phage integrase family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_01378</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Site-specific tyrosine recombinase, which acts by catalyzing the cutting and rejoining of the recombining DNA molecules.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 'phage' integrase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000147">
        <property type="protein sequence ID" value="CCZ83642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01378"/>
    <dbReference type="EnsemblBacteria" id="CCZ83642">
        <property type="protein sequence ID" value="CCZ83642"/>
        <property type="gene ID" value="BN566_01378"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044068">
        <property type="entry name" value="CB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004107">
        <property type="entry name" value="Integrase_SAM-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14659">
        <property type="entry name" value="Phage_int_SAM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51900">
        <property type="entry name" value="CB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core-binding (CB)" evidence="7">
        <location>
            <begin position="70"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Tyr recombinase" evidence="6">
        <location>
            <begin position="182"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="433" mass="50604" checksum="2F90611993A26ADA" modified="2013-07-24" version="1">MAGKVLTRHRKNRAGVYTSWEYRFQSASVSGQRKWITQAGFQTKQEAYQAGMAAYQEYQNTGVHFTPSERSVSDYMQYWMEEYCKTNLKETTILNYQKKIKNLILPAIGKYKLCSISTIALQQFINRLFQSGYARNTLVVVKGILTNAFRYAKQQKLIQENPMVEVALPRRNAVSKNPSRTKQRFCVPAEAIQQIFRRFPEGHPVHIPLLFGYRCGLRHGEAYAVQWKDIDFEAKELHIQRQIQYRESETDENGNSILYFSTPKYNSFRTVQLDDETIALLKRTKEQQEKNREAYDSYYVHYYEEAHTRNLNTVGNGEELYFVNCNADGSFIKPRTMQHASRVIHQELNLPSFDYHSLRHTHCTELLESGVPPKVVQMRLGHKDIRTTLNIYEHITKKMEENTQEILNQMYAASTLCPRSNQTQEESKKDVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5VTC5</accession>
    <name>R5VTC5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">2-oxoglutarate ferredoxin oxidoreductase gamma subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01527</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000162">
        <property type="protein sequence ID" value="CCZ83792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01527"/>
    <dbReference type="EnsemblBacteria" id="CCZ83792">
        <property type="protein sequence ID" value="CCZ83792"/>
        <property type="gene ID" value="BN566_01527"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016903">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019752">
        <property type="entry name" value="Pyrv/ketoisovalerate_OxRed_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01558">
        <property type="entry name" value="POR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="SSF53323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="POR" evidence="2">
        <location>
            <begin position="11"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="177" mass="18838" checksum="806EBB2A94E1711E" modified="2013-07-24" version="1">MTTEFILAGFGGQGILFAGKILAYCGLMAGKELSWLPSYGPEMRGGTANCSVCISDEPIGSPLVTAPNCLIAMNGPSYTKFVNTVQPNGLILLDNSVVEEEAVRDDVKTISLPATDMATENGLNGSANMILLGRMLAETKLFDLDTVQKAMEKCIPPKRAHLIEANMKAVRIGAESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>R5VTE5</accession>
    <name>R5VTE5_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phosphatidylglycerol--prolipoprotein diacylglyceryl transferase</fullName>
            <ecNumber evidence="2">2.5.1.145</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">lgt</name>
        <name evidence="4" type="ORF">BN566_01542</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the transfer of the diacylglyceryl group from phosphatidylglycerol to the sulfhydryl group of the N-terminal cysteine of a prolipoprotein, the first step in the formation of mature lipoproteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol) + L-cysteinyl-[prolipoprotein] = H(+) + S-1,2-diacyl-sn-glyceryl-L-cysteinyl-[prolipoprotein] + sn-glycerol 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:56712"/>
            <dbReference type="Rhea" id="RHEA-COMP:14679"/>
            <dbReference type="Rhea" id="RHEA-COMP:14680"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
            <dbReference type="ChEBI" id="CHEBI:64716"/>
            <dbReference type="ChEBI" id="CHEBI:140658"/>
            <dbReference type="EC" id="2.5.1.145"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; lipoprotein biosynthesis (diacylglyceryl transfer).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the Lgt family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.145" evidence="2"/>
    <dbReference type="EMBL" id="CBAR010000164">
        <property type="protein sequence ID" value="CCZ83807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01542"/>
    <dbReference type="EnsemblBacteria" id="CCZ83807">
        <property type="protein sequence ID" value="CCZ83807"/>
        <property type="gene ID" value="BN566_01542"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00664"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008961">
        <property type="term" value="F:phosphatidylglycerol-prolipoprotein diacylglyceryl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042158">
        <property type="term" value="P:lipoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01147">
        <property type="entry name" value="Lgt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001640">
        <property type="entry name" value="Lgt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30589">
        <property type="entry name" value="PTHR30589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01790">
        <property type="entry name" value="LGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00544">
        <property type="entry name" value="lgt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01311">
        <property type="entry name" value="LGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="104"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="203"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="232"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="261"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="318"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol)</name>
            <dbReference type="ChEBI" id="CHEBI:64716"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="353" mass="39039" checksum="8A178F15B1EF5C65" modified="2013-07-24" version="1">MLEQNEIQFPHLGIDLHVDSTAFQIFGIDIQWYGLLITCGLLLAMIYAFHNMRSYGIHPDRAIDAIIGGIIGAIVGARAYYVILEWDNYAGDWKRIFNLRQGGLAIYGGLIGALVVGCIIAKIRKVRLLPLLDLCGAGFLLGQGIGRWGNFFNQEAFGCNTNSIFGMTGGTIQNWITNVYPTTNFCQNFGKTMDAATPVHPCFLYESFWCLLGFVVLAICGKKFRKFDGQIFIMYIGWYGLGRFFIEGLRVDSLVIGNMRISQLIAGICVVVSVILLLVIGGRVRRMGSDYQLYCNTEACKQMMAELDAKREASGKKAAASVALDSAKEPEDETSEPEETSEAKTDAPEENDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>R5VTL0</accession>
    <name>R5VTL0_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">5-formyltetrahydrofolate cyclo-ligase</fullName>
            <ecNumber evidence="3">6.3.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00167</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(6S)-5-formyl-5,6,7,8-tetrahydrofolate + ATP = 5,10-methenyltetrahydrofolate + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10488"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:57457"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the 5-formyltetrahydrofolate cyclo-ligase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.3.2" evidence="3"/>
    <dbReference type="EMBL" id="CBAR010000168">
        <property type="protein sequence ID" value="CCZ83862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00167"/>
    <dbReference type="EnsemblBacteria" id="CCZ83862">
        <property type="protein sequence ID" value="CCZ83862"/>
        <property type="gene ID" value="BN566_00167"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030272">
        <property type="term" value="F:5-formyltetrahydrofolate cyclo-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002698">
        <property type="entry name" value="FTHF_cligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024185">
        <property type="entry name" value="FTHF_cligase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23407:SF1">
        <property type="entry name" value="PTHR23407:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01812">
        <property type="entry name" value="5-FTHF_cyc-lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006806">
        <property type="entry name" value="FTHF_cligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02727">
        <property type="entry name" value="MTHFS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="8"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="138"/>
            <end position="146"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR006806-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="197" mass="22505" checksum="A9DBD8E9FA194EEC" modified="2013-07-24" version="1">MPEQQTKKEQLRQYYRTLRKERTAQEKQQLDLAMQERFCASACYRNSNVLLLYAATIAEPETEWILSQALQDEKIVGLPKCLPERQMVFCQVTNSQQLHRGAYGILEPDAACPTLEPLPETGCVCLVPGLAFDRAGYRLGYGGGYYDRFLQQHPQLIRVGYCPSMHVTETLPREETDCPVQYLITEQTEEELYGSGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5VU34</accession>
    <name>R5VU34_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sporulation protein YunB (Spo_YunB)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01736</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000178">
        <property type="protein sequence ID" value="CCZ84007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01736"/>
    <dbReference type="EnsemblBacteria" id="CCZ84007">
        <property type="protein sequence ID" value="CCZ84007"/>
        <property type="gene ID" value="BN566_01736"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014197">
        <property type="entry name" value="Sporulation_prot_YunB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09560">
        <property type="entry name" value="Spore_YunB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02832">
        <property type="entry name" value="spo_yunB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="214" mass="23538" checksum="167D2F56C957595A" modified="2013-07-24" version="1">MRRRNTALQRKQLTWLCCSIFMIAGLLFGVVQLDRWIRPDVLAVCAQECQSAASVQIGESIHKVLQETDSQYTDFAVLLYDTDGAVTAVETKTTQINRMQAQLLTQINQDLQAARAETFPVSLGTATGIWLFAGKGPTLSMKMLPIGTAKVQLISQLESVGINQTCHTIRADVTITLQAAAPFYRTTTTAQFSYLLTETILVGKVPESYVVFGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>R5VUD7</accession>
    <name>R5VUD7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 12">Protein translocase subunit SecA</fullName>
            <ecNumber evidence="11">7.4.2.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">secA</name>
        <name evidence="17" type="ORF">BN566_01853</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Monomer and homodimer. Part of the essential Sec protein translocation apparatus which comprises SecA, SecYEG and auxiliary proteins SecDF. Other proteins may also be involved.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell membrane</location>
            <topology evidence="11">Peripheral membrane protein</topology>
            <orientation evidence="11">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="11">Distribution is 50-50.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11 12">Belongs to the SecA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.4.2.8" evidence="11"/>
    <dbReference type="EMBL" id="CBAR010000189">
        <property type="protein sequence ID" value="CCZ84127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01853"/>
    <dbReference type="EnsemblBacteria" id="CCZ84127">
        <property type="protein sequence ID" value="CCZ84127"/>
        <property type="gene ID" value="BN566_01853"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017038">
        <property type="term" value="P:protein import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18803">
        <property type="entry name" value="SF2_C_secA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01382">
        <property type="entry name" value="SecA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000185">
        <property type="entry name" value="SecA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020937">
        <property type="entry name" value="SecA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011115">
        <property type="entry name" value="SecA_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014018">
        <property type="entry name" value="SecA_motor_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011130">
        <property type="entry name" value="SecA_preprotein_X-link_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044722">
        <property type="entry name" value="SecA_SF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011116">
        <property type="entry name" value="SecA_Wing/Scaffold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036266">
        <property type="entry name" value="SecA_Wing/Scaffold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036670">
        <property type="entry name" value="SecA_X-link_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30612">
        <property type="entry name" value="PTHR30612"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07517">
        <property type="entry name" value="SecA_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01043">
        <property type="entry name" value="SecA_PP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07516">
        <property type="entry name" value="SecA_SW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00906">
        <property type="entry name" value="SECA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00957">
        <property type="entry name" value="SecA_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00958">
        <property type="entry name" value="SecA_PP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81767">
        <property type="entry name" value="SSF81767"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81886">
        <property type="entry name" value="SSF81886"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00963">
        <property type="entry name" value="secA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01312">
        <property type="entry name" value="SECA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51196">
        <property type="entry name" value="SECA_MOTOR_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 11 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-1278">Translocase</keyword>
    <keyword evidence="9 11 12" id="KW-0811">Translocation</keyword>
    <keyword evidence="2 11 12" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SECA_MOTOR_DEAD" evidence="16">
        <location>
            <begin position="1"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="87"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="434"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="860"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="103"/>
            <end position="107"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="509"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CCZ84127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="899" mass="101773" checksum="D70E775F2F8129AD" modified="2013-07-24" version="1">MGIFSKLFGSYSTRELARIEPIKQKVLALDEEYTALSDAELKGKTQEFRDRLESGETLDSMMAEALATVREAAYRVLGKKPFPVQIIGAIVLHQGRIAEMKTGEGKTLVACVASYLNALPGKGVHVVTVNDYLAKTQSDEMGKVLRFLGLTVGCILHGLTNDQRREAYACDVTYGTNNELGFDYLRDNMVIYKKDKVQREHNFAIVDEVDSILIDEARTPLIISGRGDKSTSLYGVVDKFAKTLTPTMVVEMDSKQDQEDTNENADYIIDEKAKTATITRRGVKKAEDYFAIDNLMAPENLTLLHHINQALKANGVMRKDIDYIVEDGEVVIIDEFTGRKMLGRRFNDGLHQAIEAKEGVKVARESKTLATITFQNYFRLYNKLSGMTGTAMTEEDEFREIYKLDVIAIPTNRPIKRIDRNDLVYRTEAAKYRAIIEQIIECHEKGQPVLVGTISIEKSELLSAMLKKKGIKHEVLNAKYHAKEAEIVAQAGKLGAVTIATNMAGRGTDIMLGGNAEFLARAEMRKKAYPEEIITEAIGYADTDNEEVLAAREEYQELYRKFSEEVKEKAVAVKEAGGLYILGTERHESRRIDNQLRGRSGRQGDEGESRFFLSVEDDLMRVFAGDRLENLMRTLNVDENTPIESKSLSKIIESSQKKVEGRNFSIRKNVLNYDDVMNTQREIIYKERQQVLDGEDLHDSILKMMEELISDTVKQYINEEVVDRTQWNLVGLKDHFLGWITEENDLEYEGEDLEKLTVDTLIETLTEKAKSLYAAREEQYGSDVIRELERVVMLKVVDTKWMAHIDDMSELKKGIGLRAYGQQNPVVEYRYEGFEMFDAMVASIKEDTIRMLLTMQLRQNQAPEREQVSKPDAPNAGAGDGSFGTQRRNPARAAKKNQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>R5VUH1</accession>
    <name>R5VUH1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_01897</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000193">
        <property type="protein sequence ID" value="CCZ84172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01897"/>
    <dbReference type="EnsemblBacteria" id="CCZ84172">
        <property type="protein sequence ID" value="CCZ84172"/>
        <property type="gene ID" value="BN566_01897"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01392">
        <property type="entry name" value="HTH_LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000843">
        <property type="entry name" value="HTH_LacI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046335">
        <property type="entry name" value="LacI/GalR-like_sensor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00356">
        <property type="entry name" value="LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13377">
        <property type="entry name" value="Peripla_BP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00354">
        <property type="entry name" value="HTH_LACI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50932">
        <property type="entry name" value="HTH_LACI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH lacI-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ84172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="328" mass="36950" checksum="3B6840518C38EBB8" modified="2013-07-24" version="1">MTINTIAKMAGVSPATVSRYLNQGYVSEEKKERIRNVIEQTGYSPSPSAQMLRTGKNHLVGVIVPRINSEPVAEMVEGITRIMAQAGYQILLANTSNQVEKELEFLKIFRTNNVDGVIFMGTLMSKRHLTVMRSYQKPIVLLAQQEEIIPSVYFDDYQSAYLATEILLKRDCREIAYIGVTLKDRAVGRNRRIGFTDALAQYQIQPDERRMVETQFDMEDGYEAARILMERNVPFDGIFCATAELAFGAMSYLRQQNVLVPEQVKVSAVGNNRLATLYDPPLTTVSLSHRTGGSEAAAMLLDLIQEQEIPRRSEKMICCLHERKSTLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5VUV3</accession>
    <name>R5VUV3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">mRNA interferase</fullName>
            <ecNumber evidence="3">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00215</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Toxic component of a type II toxin-antitoxin (TA) system.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the PemK/MazF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="3"/>
    <dbReference type="EMBL" id="CBAR010000210">
        <property type="protein sequence ID" value="CCZ84302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00215"/>
    <dbReference type="EnsemblBacteria" id="CCZ84302">
        <property type="protein sequence ID" value="CCZ84302"/>
        <property type="gene ID" value="BN566_00215"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003477">
        <property type="entry name" value="PemK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011067">
        <property type="entry name" value="Plasmid_toxin/cell-grow_inhib"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33988">
        <property type="entry name" value="PTHR33988"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02452">
        <property type="entry name" value="PemK_toxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF033490">
        <property type="entry name" value="MazF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-1277">Toxin-antitoxin system</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR033490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="114" mass="12653" checksum="533FA4279D0712EB" modified="2013-07-24" version="1">MMVKRGEIYYADLSPVVGSEQGGLRPVLIVQNDVGNRHSPTVIAAAITSQKDKAHLPTHIAVQADRCGLTRDSIVLLEQIRTIDKRRLKDRMGSLDRPSMHQVDEALQISFGLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>R5VUX0</accession>
    <name>R5VUX0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Bacterial regulatory proteins tetR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00230</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000212">
        <property type="protein sequence ID" value="CCZ84317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84317">
        <property type="protein sequence ID" value="CCZ84317"/>
        <property type="gene ID" value="BN566_00230"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039532">
        <property type="entry name" value="TetR_C_Firmicutes"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14278">
        <property type="entry name" value="TetR_C_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="17"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="204" mass="23359" checksum="9D8ECB36434ECB0D" modified="2013-07-24" version="1">MQQTASATPSRQERRVRRTRAMILAGFIQLMQQKPVKDISVRELADLVDINRSTFYLHYTDIYDLLEQTENGLMDQFLAIIDKNHANLTLQEFSDKLEQFFTILSENQPLCRALMSPNGDIAFVRKLEKLIAEDGVKTLRLLSDEKDLDAQDLNYVTSFFLSGCVGMIDLWLQDGCQQSAQHMADLSMKLLRAGVQGITRRQLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVC9</accession>
    <name>R5VVC9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">ATP-dependent DNA helicase RecG</fullName>
            <ecNumber evidence="3 8">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">recG</name>
        <name evidence="11" type="ORF">BN566_00356</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 8">Critical role in recombination and DNA repair. Helps process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3'- to 5'- polarity. Unwinds branched duplex DNA (Y-DNA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the helicase family. RecG subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3 8"/>
    <dbReference type="EMBL" id="CBAR010000235">
        <property type="protein sequence ID" value="CCZ84457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00356"/>
    <dbReference type="EnsemblBacteria" id="CCZ84457">
        <property type="protein sequence ID" value="CCZ84457"/>
        <property type="gene ID" value="BN566_00356"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004609">
        <property type="entry name" value="ATP-dep_DNA_helicase_RecG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045562">
        <property type="entry name" value="RecG_dom3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033454">
        <property type="entry name" value="RecG_wedge"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19833">
        <property type="entry name" value="RecG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17191">
        <property type="entry name" value="RecG_wedge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00643">
        <property type="entry name" value="recG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6 8" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8 11" id="KW-0347">Helicase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="268"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="448"/>
            <end position="607"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ84457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="680" mass="76043" checksum="086DDF5BD76889EF" modified="2013-07-24" version="1">MHRLFQPITSLKYVAQKRAKLYDKLDITTPYDLLYHLPRHYMDYRNPISIAEAQNQTLAVLRVQILQKLAPQFIRSGLTVFKAIAADDTAQISIVLYNNHFAMDALTIGSTYYLYGKIGGNLLRKEIYSPHIIPASSNQLIRPIYALTTGLTANMIETNVRQALNLLEEEPFEWMPGWLLTQYHLPLLADALPEIHFPHSMETLEQARRRLAFDELLQLQIGMQMLRKRTETAAAIPMQSISMQPFYDALPFSMTQGQQKAVSEILDSLCQPVPMNRLLQGDVGSGKTAVAAAACYFAAKNGVQSALMAPTEILAGQHYATLQRFLEPLGISVGLLTGSMKAKEKKEVRTAAQDGSLMVLVGTHAIFQKEVQFSNLALVITDEQHRFGVEQRSLLAEKGTCPHKLVMSATPIPRTLALMIYGDLDLSILGELPNGRKPIETYAVTGKLRERAYSFIQKQLEQGRQAYIVCPTIEEGDNNLQAVEQYAKQVQEGAFSAYSVGLLHGKLKANEKDAVMQAFRDNEIQVLVCTTVVEVGVDVPNATIMMIEDAERFGLSQLHQLRGRVGRGDAQSYCILVTDHVTDACRQRMQIMSRMRDGFQIAEADLKLRGPGDFFGERQHGLPPLKAADMMKDMELIRETEQAAEQLLQDDPTLQQPENQGLRLEVLRLFAKTGENGVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVE1</accession>
    <name>R5VVE1_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF6440 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00366</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000238">
        <property type="protein sequence ID" value="CCZ84467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84467">
        <property type="protein sequence ID" value="CCZ84467"/>
        <property type="gene ID" value="BN566_00366"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045515">
        <property type="entry name" value="DUF6440"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20037">
        <property type="entry name" value="DUF6440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6440" evidence="1">
        <location>
            <begin position="5"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="69" mass="7871" checksum="C6F41A3D74AE4ECF" modified="2013-07-24" version="1">MESNRFVKLYKQGSTLGSSYLEIWVDRETGVQYLYRMNGYSGGFTPLLDRDGKPLLASAEEMEQPCKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVX4</accession>
    <name>R5VVX4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Argininosuccinate lyase</fullName>
            <shortName evidence="7">ASAL</shortName>
            <ecNumber evidence="3 7">4.3.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Arginosuccinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">argH</name>
        <name evidence="10" type="ORF">BN566_00535</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>2-(N(omega)-L-arginino)succinate = fumarate + L-arginine</text>
            <dbReference type="Rhea" id="RHEA:24020"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:57472"/>
            <dbReference type="EC" id="4.3.2.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the lyase 1 family. Argininosuccinate lyase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.1" evidence="3 7"/>
    <dbReference type="EMBL" id="CBAR010000267">
        <property type="protein sequence ID" value="CCZ84682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00535"/>
    <dbReference type="EnsemblBacteria" id="CCZ84682">
        <property type="protein sequence ID" value="CCZ84682"/>
        <property type="gene ID" value="BN566_00535"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00114"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004056">
        <property type="term" value="F:argininosuccinate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01359">
        <property type="entry name" value="Argininosuccinate_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00006">
        <property type="entry name" value="Arg_succ_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029419">
        <property type="entry name" value="Arg_succ_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009049">
        <property type="entry name" value="Argininosuccinate_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43814">
        <property type="entry name" value="PTHR43814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14698">
        <property type="entry name" value="ASL_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="SSF48557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00838">
        <property type="entry name" value="argH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4 7" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lyase_1" evidence="8">
        <location>
            <begin position="6"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="ASL_C2" evidence="9">
        <location>
            <begin position="363"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF14698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ84682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="459" mass="51342" checksum="92662E4EA0393D91" modified="2013-07-24" version="1">MALWAGRFSKEIDAGVNAFNSSISFDARMYRQDIEGSIAHATMLGDCGIIPKEDSVQIIQGLKEILADLDSGKLEFDPNAEDIHMFVEAELTKRYGDVGKRLHTSRSRNDQVALDLRLYLRTEIAEIRALVRKLCKTLVNVAEQHTETVMPGYTHLQRAQPVTFAHHLLAYVQMLLRDLGRLDDTEKRMNVCPLGSGALAGTTYHINREQTAELLGFDAPMANSLDGVSDRDYCIELANAISLIMMHLSRFSEEVILWCSWEFKFIELDDAFATGSSIMPQKKNPDITELIRGKTARVYGDLQTLLVMMKGTPLAYNKDMQEDKEAIFDAVDTVKLCLETFIPMIATMTVRKDNMRAAAAKGFINATDCADYLVKKGMPFRDAYKISGTLVAKCIRENLTLETLPLEAYQEMTPLFTEDIYDAISLETCVKGRVSQGGPSPKAVAVQLEQVKNILASWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5UWS8</accession>
    <name>R5UWS8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Dockerin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00583</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000007">
        <property type="protein sequence ID" value="CCZ82778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00583"/>
    <dbReference type="EnsemblBacteria" id="CCZ82778">
        <property type="protein sequence ID" value="CCZ82778"/>
        <property type="gene ID" value="BN566_00583"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="SSF55797"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004394570" description="Dockerin domain-containing protein" evidence="1">
        <location>
            <begin position="31"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="2">
        <location>
            <begin position="215"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ82778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="289" mass="32614" checksum="5D48202287A2705B" modified="2013-07-24" version="1">MKKWKKFVFGLLFGTMICFLLPKPVLQTDAAVPFDVNEFVDIASANINAMRMENGLEPLQTAPLLQKMCQERADELTKSYAHSRANGDAWYTILTDYGIDTNCFAGENIAAGYDTPEGVVEGWMNSAGHRKNILNTNYEYFAVGVSYYEDDPEYYFYYWDLILLSSDTGFEDSYTPLAIFMPATTEETEQTTMTTLQTTTMTMTTIPETSTTLAYDYNYGDCNTDGVVDLRDAIFLGKYLSGQVAMTRIQLINANCNQLDGTEIVDEKDVQSIMRFILLKLDTLPETEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXQ7</accession>
    <name>R5UXQ7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Pyruvate formate-lyase-activating enzyme</fullName>
            <ecNumber evidence="12">1.97.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN566_00665</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 12">Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>glycyl-[formate C-acetyltransferase] + reduced [flavodoxin] + S-adenosyl-L-methionine = 5'-deoxyadenosine + glycin-2-yl radical-[formate C-acetyltransferase] + H(+) + L-methionine + semiquinone [flavodoxin]</text>
            <dbReference type="Rhea" id="RHEA:19225"/>
            <dbReference type="Rhea" id="RHEA-COMP:10622"/>
            <dbReference type="Rhea" id="RHEA-COMP:12190"/>
            <dbReference type="Rhea" id="RHEA-COMP:12191"/>
            <dbReference type="Rhea" id="RHEA-COMP:14480"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:29947"/>
            <dbReference type="ChEBI" id="CHEBI:32722"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:140311"/>
            <dbReference type="EC" id="1.97.1.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>glycyl-[protein] + reduced [flavodoxin] + S-adenosyl-L-methionine = 5'-deoxyadenosine + glycin-2-yl radical-[protein] + H(+) + L-methionine + semiquinone [flavodoxin]</text>
            <dbReference type="Rhea" id="RHEA:61976"/>
            <dbReference type="Rhea" id="RHEA-COMP:10622"/>
            <dbReference type="Rhea" id="RHEA-COMP:14480"/>
            <dbReference type="Rhea" id="RHEA-COMP:15993"/>
            <dbReference type="Rhea" id="RHEA-COMP:15994"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:29947"/>
            <dbReference type="ChEBI" id="CHEBI:32722"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:140311"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="12">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the organic radical-activating enzymes family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.97.1.4" evidence="12"/>
    <dbReference type="EMBL" id="CBAR010000012">
        <property type="protein sequence ID" value="CCZ82860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00665"/>
    <dbReference type="EnsemblBacteria" id="CCZ82860">
        <property type="protein sequence ID" value="CCZ82860"/>
        <property type="gene ID" value="BN566_00665"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043365">
        <property type="term" value="F:[formate-C-acetyltransferase]-activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034457">
        <property type="entry name" value="Organic_radical-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012839">
        <property type="entry name" value="Organic_radical_activase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012838">
        <property type="entry name" value="PFL1_activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001989">
        <property type="entry name" value="Radical_activat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30352">
        <property type="entry name" value="PTHR30352"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000371">
        <property type="entry name" value="PFL_act_enz"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02493">
        <property type="entry name" value="PFLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01087">
        <property type="entry name" value="RADICAL_ACTIVATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 12" id="KW-0408">Iron</keyword>
    <keyword evidence="11 12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="14" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="13">
        <location>
            <begin position="14"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CCZ82860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="250" mass="28718" checksum="B4E0745D0BF21812" modified="2013-07-24" version="1">MEGYIHSTESYGTVDGPGTRFVIFFQGCPMRCQYCHNPDTWTYNINEKRTVESLLHDYDGLKEFYHDGGITATGGEPLVQMEFLTELFTKAKERNINTCLDTSGILFRQEKREAFDKLMAVTDLVMLDIKHIDDTEHRKLTGQSNVHILEFAQYLKEKNIPVWIRHVVVPGITLQEKYLRQLGAYLAGLDNLKALDVLPYHTMGEVKYENLGIPYPLKGVEPATTEQAIWARNTILDALKEQLRKEKFGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5UY22</accession>
    <name>R5UY22_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Pectate lyase/Amb allergen</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01009</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000080">
        <property type="protein sequence ID" value="CCZ83228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83228">
        <property type="protein sequence ID" value="CCZ83228"/>
        <property type="gene ID" value="BN566_01009"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002105">
        <property type="entry name" value="Dockerin_1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00404">
        <property type="entry name" value="Dockerin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004394608" evidence="1">
        <location>
            <begin position="31"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="2">
        <location>
            <begin position="326"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="396" mass="44039" checksum="FB7881F20F81EAD0" modified="2013-07-24" version="1">MKRTKKLLASILASSLAVMSLPFFGLSASAGSEMVMDTTEIVKESSQTYYELISISSFDRFSYLYCNPETKTAFTIPAREFPDTLYWLLEDEKRFDDGMTCILPASTEEFKFPNAEITIAGDSMYVVNHGAIVYVDDNGEEHSFSSFSDVEQYVIQNYNVTLVDYEEVKDDNKVEYPEQTWIELVDVVKRFEDSPHKICLYHNGDTKTAFEFSTADLPNELQLLINCSDTFDDGVVYGTSSKNKTLKFPNAEITDNGTFMSAVNHGSIVFVDDNGEEHPFSGFSDLENYVIQNYDVTVVDYNKYVEDGSIVPIDATTTPVNNTTAPSYFTGDVTGNGAVDLTDAISLNKIFAGLITPTNVQTTAADINQDGSVTDEDLSILMQFLIGIRTDLTDVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5UY37</accession>
    <name>R5UY37_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted ATPase of the PP-loop superfamily implicated in cell cycle control</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01024</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000081">
        <property type="protein sequence ID" value="CCZ83243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01024"/>
    <dbReference type="EnsemblBacteria" id="CCZ83243">
        <property type="protein sequence ID" value="CCZ83243"/>
        <property type="gene ID" value="BN566_01024"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011063">
        <property type="entry name" value="TilS/TtcA_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01171">
        <property type="entry name" value="ATP_bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="SSF52821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="1">
        <location>
            <begin position="15"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="387" mass="44552" checksum="E6047546D8D5368E" modified="2013-07-24" version="1">MVQEEITLEALEAQPKDTYLLVDIRDEAAFQFGHMDGAIHIPANELPERHAELPKDQLLVIYCRSGIISEDAAAELREDGFSAVNLVGGYVQWLQRKIRRETTGEKAAEIEQSLRKKFHRVIFSRFAKAVNTYDLIQPNDRIAVCISGGKDSMLMAKLFQELQRHGKFPFELVFLVMDPGYSPENRAIIESNAKLLQIPITVFETNIFNSVYNVENNPCYLCARMRRGHLYHQAEVLGCNKIALGHHFDDVIETILMSMIYGGQVETMMPKLHSANFPGMQLIRPMYLIREADIKHWCSYHNLRFIQCACRFTDTCTTCNPSSNTSSKRQKIKQLIAELAQDNPQIEKNIFRSVENVHLNKIISYQQNKQFHSFLDDYDNHPEKPKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYE3</accession>
    <name>R5UYE3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted O-methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00833</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000040">
        <property type="protein sequence ID" value="CCZ83040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00833"/>
    <dbReference type="EnsemblBacteria" id="CCZ83040">
        <property type="protein sequence ID" value="CCZ83040"/>
        <property type="gene ID" value="BN566_00833"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006807">
        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071704">
        <property type="term" value="P:organic substance metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MTS" evidence="1">
        <location>
            <begin position="15"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="245" mass="27800" checksum="42ED9FB4BA016CCF" modified="2013-07-24" version="1">MSSYRKEPLTGQMHVYVSENHRFGTDAFLLANFSNWRKKDLVCDLGTGCGIIPMILQRKNPPKHTWGVDIQADAIDQFSQAITECGVQDRVTPVCADLKQLWQDAPLERCDLVTCNPPYKAYQAGMESQLTAQKIARHEILCNIADVCQAASRLLKFGGRLCICNRSERLADCIAAMQQFHIEPKRLQFVSKNPDTAPWLFLLEGRKGGKPFLNVLPQFYIRDGETDSEAVRNLYRLPEEQEVQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYG4</accession>
    <name>R5UYG4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Hydrolase TatD family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00853</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000048">
        <property type="protein sequence ID" value="CCZ83065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00853"/>
    <dbReference type="EnsemblBacteria" id="CCZ83065">
        <property type="protein sequence ID" value="CCZ83065"/>
        <property type="gene ID" value="BN566_00853"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004536">
        <property type="term" value="F:deoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01310">
        <property type="entry name" value="TatD_DNAse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015991">
        <property type="entry name" value="Hydrolase_TatD-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001130">
        <property type="entry name" value="TatD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01026">
        <property type="entry name" value="TatD_DNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005902">
        <property type="entry name" value="DNase_TatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00010">
        <property type="entry name" value="TIGR00010"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="256" mass="28501" checksum="A816E8159D6723F2" modified="2013-07-24" version="1">MERLIFDTHSHYMDDAFVEDRDAVLQALPEGGVALAMLASVDAADSEQNLKLTEQYDYLLGSVGIHPENLPEHPAEELERIAALAAHKNCYAIGEIGLDYHYEGYDKEAQIAIFQEQITLAKELDLPVIVHIRDATADALQILKDRKPKGVVHCFSGSAETAEEVLKLGMYLGFTGILTFKNAKKALRALSVIPVDRFVLETDCPYMAPVPYRGKRCDSRMIIETAKAAAAVKELPVETILQQTLENGKRLYELNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYL0</accession>
    <name>R5UYL0_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Valine--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Valyl-tRNA synthetase</fullName>
            <shortName evidence="8">ValRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">valS</name>
        <name evidence="13" type="ORF">BN566_01179</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a 'posttransfer' editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val)</text>
            <dbReference type="Rhea" id="RHEA:10704"/>
            <dbReference type="Rhea" id="RHEA-COMP:9672"/>
            <dbReference type="Rhea" id="RHEA-COMP:9708"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78537"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.9"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">The C-terminal coiled-coil domain is crucial for aminoacylation activity.</text>
    </comment>
    <comment type="domain">
        <text evidence="8">ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.9" evidence="8"/>
    <dbReference type="EMBL" id="CBAR010000096">
        <property type="protein sequence ID" value="CCZ83403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01179"/>
    <dbReference type="EnsemblBacteria" id="CCZ83403">
        <property type="protein sequence ID" value="CCZ83403"/>
        <property type="gene ID" value="BN566_01179"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004832">
        <property type="term" value="F:valine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006438">
        <property type="term" value="P:valyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07962">
        <property type="entry name" value="Anticodon_Ia_Val"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02004">
        <property type="entry name" value="Val_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033705">
        <property type="entry name" value="Anticodon_Ia_Val"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037118">
        <property type="entry name" value="Val-tRNA_synth_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019499">
        <property type="entry name" value="Val-tRNA_synth_tRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002303">
        <property type="entry name" value="Valyl-tRNA_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11946">
        <property type="entry name" value="PTHR11946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10458">
        <property type="entry name" value="Val_tRNA-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00986">
        <property type="entry name" value="TRNASYNTHVAL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="SSF46589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="SSF50677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00422">
        <property type="entry name" value="valS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="tRNA-synt_1" evidence="10">
        <location>
            <begin position="18"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="domain" description="Anticodon_1" evidence="11">
        <location>
            <begin position="606"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="domain" description="Val_tRNA-synt_C" evidence="12">
        <location>
            <begin position="808"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="802"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="8">
        <location>
            <begin position="46"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="8">
        <location>
            <begin position="524"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="527"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF10458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CCZ83403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="871" mass="99929" checksum="BF1FCBFD35CBD6E1" modified="2013-07-24" version="1">MKKELAKSYQPKDFEDRIYAEWMEKGCFHAEVDSKKQPYTIVIPPPNITGQLHMGHALDETLQDILIRWKRMSGYSALWLPGTDHASIATEAKIVEAMRKEGIKKEDLGRDGFLKRAWDWKKQYGGRIVEQLKKLGSSCDWERERFTMDEGCNQAVKEVFIRYYEEGLIYRGERIINWCPKCLTSISDAEVEYEDQAGHFWHLRYPLKDGSGYLELATTRPETLLGDTAVAVNPNDERYQHLVGKTLILPLVHREIPIVADDYVEMDFGTGVVKITPAHDPNDFEVGLRHNLPVINVMTDDAKITDDYPKYAGMDRYEARKAIVADLEAEGALVEIEDYNHNVGTCYRCGTTVEPRVSKQWFVKMKPLAQPAIDAVRNGKTKFVPERFDKIYFHWLENIKDWCISRQLWWGHRIPAFYCDDCGEMVVTKENAATCPKCGKPMRQDPDTLDTWFSSALWPFSTLGWPNQTEELKYFYPTNTLVTGYDIIFFWVVRMMFSGLKCMNEVPFDTVLIHGLVRDEQGRKMSKSLGNGIDPLKVIDEYGADALRFMLATGNAPGNDMRYSDDKVKAARNFANKLWNASRFIMMNLPEDFQFNGLPEQLTMEDRWIVDRFNNLAKEVNDNLNKFEIGVACSKLYDFIWDVYCDWYIELTKPRIQAGGATMEQAQAVLVWVMRGMLKLLHPFMPYITEEIWQVLTNEESMIMLESYPVYTEEHAYAEAAQFEKVIDAIRAIRNCRTSMNVPPSKKASVYIETQDTELFAAAAVFFEKLASASSVEVGTSFDLQDAATAVTDSARIFIPMAELVDREKELARLQKEQQKAQKDIDFLQKKLNNPGFLAKAPEQLVAAERAKLAKATEKMEKILQSIAGLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYS2</accession>
    <name>R5UYS2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phage terminase large subunit PBSX family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01232</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000109">
        <property type="protein sequence ID" value="CCZ83458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01232"/>
    <dbReference type="EnsemblBacteria" id="CCZ83458">
        <property type="protein sequence ID" value="CCZ83458"/>
        <property type="gene ID" value="BN566_01232"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006437">
        <property type="entry name" value="Phage_terminase_lsu"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01547">
        <property type="entry name" value="phage_term_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="420" mass="48089" checksum="DB12CC8558E7421D" modified="2013-07-24" version="1">MQTTFKPFSPKQKLALRWWALPQYQDYDAIICDGAVRSGKTVCMSLGFVCWATTCFQGTAFALCGKTITALRRNVVTPLLQTLQTLGFSCTEKSSRNYVEIALGNRSNRFYLFGGKDESSAALIQGITLGGVFLDEVALMPRTFVEQALARCSLHTAKMWFNCNPDHPYHWFYREWIQKAAQKRALYLHFTMADNPGLSERVRQRYERLYSGIFYDRFVLGKWTVSSGVVYPMFSKERHVVANKPECEQFYISCDYGTVNPSSFGLWGYCKTDQVWYRLQEYYYDARKEGCSRTDEAHYTALEALAGNRTIEAVIIDPSAASMIACIAQHGRFRVIRADNDVLAGIQRVSTALQQEQIRFSAACTDTLREFGMYCWEDARHGDAPKKEFDHAMDDVRYFVSTVLRRKAEDDFFTAAIGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZ40</accession>
    <name>R5UZ40_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transcriptional regulator BadM/Rrf2 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01096</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01096"/>
    <dbReference type="EnsemblBacteria" id="CCZ83315">
        <property type="protein sequence ID" value="CCZ83315"/>
        <property type="gene ID" value="BN566_01096"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000944">
        <property type="entry name" value="Tscrpt_reg_Rrf2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33221">
        <property type="entry name" value="PTHR33221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02082">
        <property type="entry name" value="Rrf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00738">
        <property type="entry name" value="rrf2_super"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51197">
        <property type="entry name" value="HTH_RRF2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="140" mass="15215" checksum="FAD0F82F9EDCC2CB" modified="2013-07-24" version="1">MIISTKGRYALKLMMYLAQHADGSFIPLKIISTEVNVSLKYLERITTALSKQHLVESASGKGGGYRLSRKPEAYCVGEILRAVEESIEPVTCIVQGAPICETADSCQVLPIWKGLSGVINAYLDGITLAEVANWKGEPET</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZ57</accession>
    <name>R5UZ57_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Binding-protein-dependent transport systems inner membrane component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_01366</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000144">
        <property type="protein sequence ID" value="CCZ83603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01366"/>
    <dbReference type="EnsemblBacteria" id="CCZ83603">
        <property type="protein sequence ID" value="CCZ83603"/>
        <property type="gene ID" value="BN566_01366"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="22"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="119"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="153"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="212"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="257"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="82"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ83603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="291" mass="32414" checksum="49ADD8E874B06E08" modified="2013-07-24" version="1">MAAATTMPSNVGRKKKIHPFHIFKYVFLILASIISIFPFLWMIICATNDAVDINRGTVTFGTSLFKNLQNLFATDRGFLKALGNSAIIAVITTVLALLVSSAAGYGFEMFRTKNRDRVFNFLLLSMMIPFATLMIPLFRIFGKLSDSGLSFMGLNTFAAVIIPAISTAFLIFFFRQNTKSFPKDIIEAARLDGLNELQIFFRMYMPVMKSSYAAAAIITFMNSWNNYLWPLVALQSPEKKTVPLILSTMGASYNPDYGMIMAGIVIATLPTALIFFLMQKQFVQGMIGSVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZ61</accession>
    <name>R5UZ61_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative Ser/Thr protein kinase KinX</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01111</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000089">
        <property type="protein sequence ID" value="CCZ83330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83330">
        <property type="protein sequence ID" value="CCZ83330"/>
        <property type="gene ID" value="BN566_01111"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="225" mass="24615" checksum="DD7B2BE3F80E4BBD" modified="2013-07-24" version="1">MINSDCSTALAVSGLASSDKKYFPQCSQVQYSLFPSSVQVGALASTFVSLWLAGITVSESSTALAVSGLVSSDKKYFPQCSQVQYSLLPFSVQVTALAAVFFKICDAETKFACSPKSLFFKEDLQLREMVFKRVQFLNTFPPMLVTLSGIEMLVKIRHSSNAESPILVTLSGIVMLVNSLQPENAKFPILMTPSGIVMLVNLVQLRNARFPMLVTLFGMVMLVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZ81</accession>
    <name>R5UZ81_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Polyphosphate kinase</fullName>
            <ecNumber evidence="1">2.7.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_01374</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible transfer of the terminal phosphate of ATP to form a long-chain polyphosphate (polyP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[phosphate](n) + ATP = [phosphate](n+1) + ADP</text>
            <dbReference type="Rhea" id="RHEA:19573"/>
            <dbReference type="Rhea" id="RHEA-COMP:9859"/>
            <dbReference type="Rhea" id="RHEA-COMP:14280"/>
            <dbReference type="ChEBI" id="CHEBI:16838"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.1"/>
        </reaction>
    </comment>
    <comment type="PTM">
        <text evidence="1">An intermediate of this reaction is the autophosphorylated ppk in which a phosphate is covalently linked to a histidine residue through a N-P bond.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the polyphosphate kinase 1 (PPK1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.1" evidence="1"/>
    <dbReference type="EMBL" id="CBAR010000147">
        <property type="protein sequence ID" value="CCZ83638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01374"/>
    <dbReference type="EnsemblBacteria" id="CCZ83638">
        <property type="protein sequence ID" value="CCZ83638"/>
        <property type="gene ID" value="BN566_01374"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009358">
        <property type="term" value="C:polyphosphate kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008976">
        <property type="term" value="F:polyphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006799">
        <property type="term" value="P:polyphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1840.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003414">
        <property type="entry name" value="PP_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041108">
        <property type="entry name" value="PP_kinase_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024953">
        <property type="entry name" value="PP_kinase_middle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036830">
        <property type="entry name" value="PP_kinase_middle_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025200">
        <property type="entry name" value="PPK_C_dom2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30218">
        <property type="entry name" value="PTHR30218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02503">
        <property type="entry name" value="PP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13090">
        <property type="entry name" value="PP_kinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17941">
        <property type="entry name" value="PP_kinase_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03705">
        <property type="entry name" value="poly_P_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PP_kinase" evidence="2">
        <location>
            <begin position="49"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="PP_kinase_C_1" evidence="4">
        <location>
            <begin position="256"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="PP_kinase_C" evidence="3">
        <location>
            <begin position="427"/>
            <end position="597"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="622" mass="71170" checksum="498D618427A89455" modified="2013-07-24" version="1">MTCREQLLAIFERVRQMLHDKDNTYRDLQNCLKTEGVEIVDFHTISQQEADYLQSYFRSEIEPLLSPQIVSKKQPFPFLQNKEIYAVVVLEKKGSEKIGIVPCSSPVFPKYVSLPNQKNRFILTEELILHFASDIFSRHVIKSKSLIRIVRSADIDAEEENIEDETDYRAAMEKLLRARKRLRPVKMEFSRLMDPSVIAKLCEYLHLNEAQVFHSEAPLSLSFVSQVRDLLRKNRSLFYPRRVPQRSANIDDHHSMIAQIQKKDRFLSFPFESIRPFLNLLHEAGEDPHVVSIKMTLYRVATNSKIVEALIEAAENGKDVVVLVELRARFDEENNIEWSRRLEEAGCRIIYGLDNLKVHSKLCLITRKIGNSISYITQVGTGNYNEKTSEIYTDYSLMTARPEIGMEASRVFNALCMGQTIKHTEYLLVSPHCLQNHVADRIDVEIEKAKAGQPAYIGLKVNSLTDKYLIDKLIEASQAGVKIEMIIRGICCLIAGIPGQTENITIRSIVGRYLEHTRIYIFGAGNQADVYIASADWMTRNTIRRVEVGAPIYDKDIKSRILSMFRIMMQDNVKARIQQPDGSYKKVQSNASPLNAQEYFYAQAYASAKAIATAETEVEAEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZ97</accession>
    <name>R5UZ97_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01136</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000092">
        <property type="protein sequence ID" value="CCZ83360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01136"/>
    <dbReference type="EnsemblBacteria" id="CCZ83360">
        <property type="protein sequence ID" value="CCZ83360"/>
        <property type="gene ID" value="BN566_01136"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="151" mass="16749" checksum="050C669BB5442D1E" modified="2013-07-24" version="1">MGSKLDTSESIGNEYGVSKGSVVRLIRINKLTDELKALVDSGDIAIRTGVELSFLSEDTQAVVAECAEDCKIDMKNAKKLRASADSEGNITPETVHAILYDEDTEPKVKPKSVKISHDIYTKYFSNGEKPKEITDTIEKALELYFKNMEDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZH1</accession>
    <name>R5UZH1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">FOG: FHA domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01191</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000099">
        <property type="protein sequence ID" value="CCZ83415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01191"/>
    <dbReference type="EnsemblBacteria" id="CCZ83415">
        <property type="protein sequence ID" value="CCZ83415"/>
        <property type="gene ID" value="BN566_01191"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00060">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SSF49879"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="FHA" evidence="2">
        <location>
            <begin position="74"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="153" mass="17720" checksum="19C1DF5673C615C9" modified="2013-07-24" version="1">MYEELLKTLRDFTPFSIIVSAVVIFVVFLCVFVIFSFAKREKRIEQLRWNKVSNHMMLRDVQTNEWIPLEADEILVGRHGSADIRLPDMSISRYHAVLYVSNGVWRVVDLHSKSGTFVNGTKIDHQAILKPMDEIRFGTQAVILQQRRDANVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZR9</accession>
    <name>R5UZR9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01264</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000117">
        <property type="protein sequence ID" value="CCZ83495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01264"/>
    <dbReference type="EnsemblBacteria" id="CCZ83495">
        <property type="protein sequence ID" value="CCZ83495"/>
        <property type="gene ID" value="BN566_01264"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="9"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="165" mass="17817" checksum="EC4B24C66EA94DBC" modified="2013-07-24" version="1">MLTAALLQYERSKSQSPLTPSATQTEPATTEESVQIPNPFIPCATLQAAADIAGFSLDAPDAYEAYDHTTIQAIEGEMIEVIYEDASENEGLRIRKGIGTDPISGDYNRYDSEETQTIAGVSVSVRKNGDLIFVAEWTDGGYAHSITSENGLTADELETLVSEVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZW2</accession>
    <name>R5UZW2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phosphatase YbjI</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01622</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000170">
        <property type="protein sequence ID" value="CCZ83893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01622"/>
    <dbReference type="EnsemblBacteria" id="CCZ83893">
        <property type="protein sequence ID" value="CCZ83893"/>
        <property type="gene ID" value="BN566_01622"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000150">
        <property type="entry name" value="Cof"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00099">
        <property type="entry name" value="Cof-subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="272" mass="30077" checksum="7AEFCC1B5A2D4A3E" modified="2013-07-24" version="1">MVKLIAADMDGTLLDDQKRLPNGFPKLLECLAKRGVAFAVASGRSYPALQMLFGKKTEELLLICDNGAHVRIPGKAPVYQCMPFSVVHAILDLCQKLPSVVPVLCGVDGIYYPKAAKSQFEQEITNFYVQFSAIPYAELYTVTDPIIKIALCAMDGSLEHAYTALRERFGENYEIVISGDVWMDMMCKQVSKGNALSDIQSLLQVSPAETMAFGDYQNDISMFAKADYSYAMGQASDAVKQHARFVAPPNTENGVVRTICEVLQITEKEFMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZZ1</accession>
    <name>R5UZZ1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted membrane-bound metal-dependent hydrolase (DUF457)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01647</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000172">
        <property type="protein sequence ID" value="CCZ83918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01647"/>
    <dbReference type="EnsemblBacteria" id="CCZ83918">
        <property type="protein sequence ID" value="CCZ83918"/>
        <property type="gene ID" value="BN566_01647"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007404">
        <property type="entry name" value="YdjM-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04307">
        <property type="entry name" value="YdjM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="183"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="208" mass="22848" checksum="70E8FF29BBB1DD91" modified="2013-07-24" version="1">MTGKTHLTVGTAATILITQPSDWKSFLLCMGAAAVGSCVSDIDVSTSGSHKGLQRILILIGLAIIVVSVLDGIFHAGIWTMIQQKNGLLQSLAGFAMFIAICVYGERQPHRTFTHSLAGVATLGLAVFIMLPEAVWYFICGMGTHIVLDLFNCKKIQLLFPFERGKICFYLCKAGGKVNQSLFWVGLVLWILEILWMAATWVMEHGWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5V011</accession>
    <name>R5V011_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01662</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000173">
        <property type="protein sequence ID" value="CCZ83933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01662"/>
    <dbReference type="EnsemblBacteria" id="CCZ83933">
        <property type="protein sequence ID" value="CCZ83933"/>
        <property type="gene ID" value="BN566_01662"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027304">
        <property type="entry name" value="Trigger_fact/SurA_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109998">
        <property type="entry name" value="SSF109998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004394854" evidence="2">
        <location>
            <begin position="36"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="262"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="262"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="323"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="365"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="483" mass="53593" checksum="F2CC7002DF6AE40F" modified="2013-07-24" version="1">MEERDTMIKWKKLAATVVAAAAAVSCVGCGNQALAYPLTIDGVKIKAGIYIYYSYQAYTEATSTIQKQNSDLDTTDEEVVKAQTIDGKDAETWIKDRTMEYCKEFVAIQKDFDAKGLELSTEQTNQVKNYISSAWDSSGETFEKNGIAESSVQDIFLSTYKEQELFQHYYGADGEKGVTEDELKEYYEDNDARVRYITFNLKDGNGDLLKDDGKKEMKSMVEDYLSVLKTLKDEDAINDKMDTIQEEYNAYVTSISEEAAAATATSATDENGNEIPATTTATTATTTSAETTTVTTVAADAESDSEAETTADTEETVTRAASDADSSDETTTTTVSGATEEESSDTTETTTTTAPYANESIITKVSTTDEKDKDNEDSINYTPSKKAYEAIFNDAKTDVPFIVEDDEAYYLIVRFDITKRMTDDDLWTEDQMKAVASRKYWDNFTDDKKEMANALNIDQQSDRAIKKYDPFKLDFSSSSSSSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5V064</accession>
    <name>R5V064_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01376</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000147">
        <property type="protein sequence ID" value="CCZ83640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83640">
        <property type="protein sequence ID" value="CCZ83640"/>
        <property type="gene ID" value="BN566_01376"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004394875" evidence="1">
        <location>
            <begin position="32"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="438" mass="48424" checksum="5D2516E04955327A" modified="2013-07-24" version="1">MKIRSMLAAACTCLITSGSVLPFTTALPAQAAFDLGDFDGNDSIDVDDAVSVLTFYAKTAAGVASIEDISNEQFNAADIDWDGSITVQDAVYILTYYAQTSAGLEPSWADILPEVYGIPAWETAYYDLLLSGALSDESGNATYSLIYIDQDDIPELLMDSYAGGKLYTYKEDTGCSLVHSWASARSSGFCGYAEGTGYFYTFSSASAFIGSMGKQELVGDSFVLRDRISMDNGTYQHNGVSCTKSQYNSIEKSYTDSYSDYYKYNFLEFMSDISDGRTPDFNQLKTALESYRPVYAMEVTDYDGDGKEEAFVVLGKKNSSSKTVQGIYYISSDGYISEVRTDFSVDMFSNANYVEYDGKGFFACDVSGGGSGWVTYLYDVRNGWWNELNLSGSLQSFFKKDDTCYTTKSVFSAQYGHQYVDVPLNYDKDTQEFSYPES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>R5V084</accession>
    <name>R5V084_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">1-deoxy-D-xylulose 5-phosphate reductoisomerase</fullName>
            <shortName evidence="7">DXP reductoisomerase</shortName>
            <ecNumber evidence="7">1.1.1.267</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">1-deoxyxylulose-5-phosphate reductoisomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">2-C-methyl-D-erythritol 4-phosphate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">dxr</name>
        <name evidence="11" type="ORF">BN566_01722</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>2-C-methyl-D-erythritol 4-phosphate + NADP(+) = 1-deoxy-D-xylulose 5-phosphate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:13717"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57792"/>
            <dbReference type="ChEBI" id="CHEBI:58262"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.267"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 1/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the DXR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.267" evidence="7"/>
    <dbReference type="EMBL" id="CBAR010000178">
        <property type="protein sequence ID" value="CCZ83993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01722"/>
    <dbReference type="EnsemblBacteria" id="CCZ83993">
        <property type="protein sequence ID" value="CCZ83993"/>
        <property type="gene ID" value="BN566_01722"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00056">
        <property type="reaction ID" value="UER00092"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030604">
        <property type="term" value="F:1-deoxy-D-xylulose-5-phosphate reductoisomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070402">
        <property type="term" value="F:NADPH binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019288">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00183">
        <property type="entry name" value="DXP_reductoisom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003821">
        <property type="entry name" value="DXP_reductoisomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013644">
        <property type="entry name" value="DXP_reductoisomerase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013512">
        <property type="entry name" value="DXP_reductoisomerase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026877">
        <property type="entry name" value="DXPR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036169">
        <property type="entry name" value="DXPR_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30525">
        <property type="entry name" value="PTHR30525"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08436">
        <property type="entry name" value="DXP_redisom_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02670">
        <property type="entry name" value="DXP_reductoisom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13288">
        <property type="entry name" value="DXPR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006205">
        <property type="entry name" value="Dxp_reductismrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69055">
        <property type="entry name" value="SSF69055"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00243">
        <property type="entry name" value="Dxr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6 7" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 7" id="KW-0521">NADP</keyword>
    <keyword evidence="5 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DXP_reductoisom" evidence="8">
        <location>
            <begin position="5"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="DXP_redisom_C" evidence="9">
        <location>
            <begin position="143"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="DXPR_C" evidence="10">
        <location>
            <begin position="258"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="149"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ83993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="382" mass="41403" checksum="44986063951624B6" modified="2013-07-24" version="1">MKKIITLLGSTGSIGTQTLDVVRMHGLEVYALAAYHNVSLLEQQVRKFHPKKVCIFDESCYPALRDALSDVSVEILTGMDGLCELASDTHANLVLNSVVGMVGLQPTLAAIAAGIDVALANKETLVAGGELVMKAAAEKGVAILPVDSEHSAIFQCLQGNAHNPVKEILLTASGGPFYGYTREQLQTVTAADALKHPNWNMGNKVTIDSATMMNKGLECLEAKWLFDLQPEQITVLVHRQSVMHSGVMYADNSVIAQLGVPDMRLPIQYAILYPERKPCPVAPLSLTQYATLTFAEPDEQTFRCLALAKKAMQAGGLLPCVMNGANEIAVQAFLQGKIGFLEIADYVETAMRTVETPELHTCADVLKTDQKARELVQNLIQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0B0</accession>
    <name>R5V0B0_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 14">ATP phosphoribosyltransferase</fullName>
            <shortName evidence="14">ATP-PRT</shortName>
            <shortName evidence="14">ATP-PRTase</shortName>
            <ecNumber evidence="5 14">2.4.2.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">hisG</name>
        <name evidence="16" type="ORF">BN566_01727</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13 14">Catalyzes the condensation of ATP and 5-phosphoribose 1-diphosphate to form N'-(5'-phosphoribosyl)-ATP (PR-ATP). Has a crucial role in the pathway because the rate of histidine biosynthesis seems to be controlled primarily by regulation of HisG enzymatic activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + ATP</text>
            <dbReference type="Rhea" id="RHEA:18473"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:73183"/>
            <dbReference type="EC" id="2.4.2.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 14">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Heteromultimer composed of HisG and HisZ subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="14">Lacks the C-terminal regulatory region which is replaced by HisZ.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the ATP phosphoribosyltransferase family. Short subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.17" evidence="5 14"/>
    <dbReference type="EMBL" id="CBAR010000178">
        <property type="protein sequence ID" value="CCZ83998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01727"/>
    <dbReference type="EnsemblBacteria" id="CCZ83998">
        <property type="protein sequence ID" value="CCZ83998"/>
        <property type="gene ID" value="BN566_01727"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00006"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003879">
        <property type="term" value="F:ATP phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13595">
        <property type="entry name" value="PBP2_HisGs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01018">
        <property type="entry name" value="HisG_Short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013820">
        <property type="entry name" value="ATP_PRibTrfase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001348">
        <property type="entry name" value="ATP_PRibTrfase_HisG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024893">
        <property type="entry name" value="ATP_PRibTrfase_HisG_short"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21403">
        <property type="entry name" value="PTHR21403"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01634">
        <property type="entry name" value="HisG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00070">
        <property type="entry name" value="hisG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14 16" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12 14" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="10 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 14 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HisG" evidence="15">
        <location>
            <begin position="54"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00020998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CCZ83998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="209" mass="22989" checksum="E9BFD7C4D24D81CA" modified="2013-07-24" version="1">MTKPLRIALTKGRLEKDTVGLLEKLGYDCEAVHNKGRRLILPIPDGNMEVVLSKAADVITYVEHGVCDLGVVGKDTILEMSGKFFELADLGFGKCRFALATKKGTDFYSGYGVKCIATKYPNIARRFFESKGMDVDIVKIEGSVELAPLLELSDGIVDIVETGVTLKENGLEVVEEIVPISSRLIANVVSLKMRQQEIESLIQKIEEVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0C2</accession>
    <name>R5V0C2_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UvrABC system protein C</fullName>
            <shortName evidence="6">Protein UvrC</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Excinuclease ABC subunit C</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">uvrC</name>
        <name evidence="10" type="ORF">BN566_01451</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrC both incises the 5' and 3' sides of the lesion. The N-terminal half is responsible for the 3' incision and the C-terminal half is responsible for the 5' incision.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with UvrB in an incision complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the UvrC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000155">
        <property type="protein sequence ID" value="CCZ83715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01451"/>
    <dbReference type="EnsemblBacteria" id="CCZ83715">
        <property type="protein sequence ID" value="CCZ83715"/>
        <property type="gene ID" value="BN566_01451"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009380">
        <property type="term" value="C:excinuclease repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009381">
        <property type="term" value="F:excinuclease ABC activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00203">
        <property type="entry name" value="UvrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000305">
        <property type="entry name" value="GIY-YIG_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035901">
        <property type="entry name" value="GIY-YIG_endonuc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001943">
        <property type="entry name" value="UVR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036876">
        <property type="entry name" value="UVR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004791">
        <property type="entry name" value="UvrC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001162">
        <property type="entry name" value="UvrC_RNase_H_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038476">
        <property type="entry name" value="UvrC_RNase_H_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01541">
        <property type="entry name" value="GIY-YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08459">
        <property type="entry name" value="UvrC_HhH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00465">
        <property type="entry name" value="GIYc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46600">
        <property type="entry name" value="SSF46600"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="SSF47781"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82771">
        <property type="entry name" value="SSF82771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00194">
        <property type="entry name" value="uvrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50164">
        <property type="entry name" value="GIY_YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50165">
        <property type="entry name" value="UVRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3 6" id="KW-0228">DNA excision</keyword>
    <keyword evidence="5 6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4 6" id="KW-0267">Excision nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0742">SOS response</keyword>
    <feature type="domain" description="GIY-YIG" evidence="8">
        <location>
            <begin position="17"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="UVR" evidence="7">
        <location>
            <begin position="206"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="UVRC" evidence="9">
        <location>
            <begin position="257"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ83715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="608" mass="68527" checksum="3955986E4EEE5759" modified="2013-07-24" version="1">MNPRLPFLREKTAKLTTSPGVYLMKNEKKTIIYVGKAKNLKNRVTSYFRVSADHTPKVASMVASVYDYDFIVTDSEYEALVLECSLIKQYQPKYNILLKDDKGYHYIFISDEPFPRFSVQQNKEKKGTYLGPYMSGFVAKEAVKEVNKVFRLPTCQKSFPASFHRGRPCLNYHLQQCMGLCRGNISQKAYQEIIQQAIAYLKNGSTASVQQMQQEMEAAAERLDFERAAILRDRMKAIAKAGETQKILDSDIKEADVLAVSESGGKQCISLLLYRNRRLFDKQTYLFSQVESTAGLLASFIGQYYDQAEKIPSDLLLPVPLENMELMQKMLSERAGHRVRLSVPQRGAAAHILQLSKHNANETLALQMSRTGKEIQALEELKQVLGLPATPEWIEAYDISNLAATSMVAGMVVFQKGKPAKKAYKRFSLEQMPGQDDYACMQQVLRRRLLHLVKQDGDAGFCKTPDLILLDGGQGHVHAVQTVLEELQLHIPLYGMVKDQKHRTRAIAKDGGEITVSRTSAAFHFLTQVQDEVHRYAITYMRSHHKKTSYALELTQVKGIGEKKAQALLVQFKTKEQLKAASAEELAKAAGVSQAVGEALYTFIQQEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0E8</accession>
    <name>R5V0E8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CarD_TRCF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01779</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000182">
        <property type="protein sequence ID" value="CCZ84053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01779"/>
    <dbReference type="EnsemblBacteria" id="CCZ84053">
        <property type="protein sequence ID" value="CCZ84053"/>
        <property type="gene ID" value="BN566_01779"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036101">
        <property type="entry name" value="CarD-like/TRCF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003711">
        <property type="entry name" value="CarD-like/TRCF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042215">
        <property type="entry name" value="CarD-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02559">
        <property type="entry name" value="CarD_CdnL_TRCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01058">
        <property type="entry name" value="CarD_TRCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141259">
        <property type="entry name" value="SSF141259"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CarD_TRCF" evidence="1">
        <location>
            <begin position="2"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="160" mass="18949" checksum="5D582E1C4ED65F91" modified="2013-07-24" version="1">MEYPIHSVVMHITTGVCRIQDIQEKQFTEDQHQKYYVLQPIFEKGTTLFVPIENDPVRIRPLLTKEAITELLHELSAQEDEPWIHNQHQRTAHFKTILKNGNEQEILSMLHMMQKHRKLPIVEERMRQTAKKLVEQEFSYVLGMKPETVPAWIRAQLQQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0F3</accession>
    <name>R5V0F3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">50S ribosomal protein L35</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpmI</name>
        <name evidence="6" type="ORF">BN566_01485</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the bacterial ribosomal protein bL35 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000161">
        <property type="protein sequence ID" value="CCZ83750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01485"/>
    <dbReference type="EnsemblBacteria" id="CCZ83750">
        <property type="protein sequence ID" value="CCZ83750"/>
        <property type="gene ID" value="BN566_01485"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00514">
        <property type="entry name" value="Ribosomal_L35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021137">
        <property type="entry name" value="Ribosomal_L35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018265">
        <property type="entry name" value="Ribosomal_L35_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001706">
        <property type="entry name" value="Ribosomal_L35_non-mt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037229">
        <property type="entry name" value="Ribosomal_L35_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01632">
        <property type="entry name" value="Ribosomal_L35p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00064">
        <property type="entry name" value="RIBOSOMALL35"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143034">
        <property type="entry name" value="SSF143034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00001">
        <property type="entry name" value="rpmI_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00936">
        <property type="entry name" value="RIBOSOMAL_L35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ83750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="67" mass="7646" checksum="F9068CE548ABE8B8" modified="2013-07-24" version="1">MAKIKTKTHSGAKKRFKVTANGKVKYQKTNKRHRLTQKDTKRKRIARTVGIADVTNAPALKKLLPYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0F5</accession>
    <name>R5V0F5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01789</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000184">
        <property type="protein sequence ID" value="CCZ84063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01789"/>
    <dbReference type="EnsemblBacteria" id="CCZ84063">
        <property type="protein sequence ID" value="CCZ84063"/>
        <property type="gene ID" value="BN566_01789"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016852">
        <property type="term" value="F:sirohydrochlorin cobaltochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019251">
        <property type="term" value="P:anaerobic cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010388">
        <property type="entry name" value="Anaerobic_Co-chelatase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06180">
        <property type="entry name" value="CbiK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004406634" evidence="2">
        <location>
            <begin position="30"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="348" mass="38083" checksum="1E10154863EE1D29" modified="2013-07-24" version="1">MKKVTIFMLSLAMTAAMLSGCSSSNDSSSSSTAETTTAAETTVADETTETEAAEETSQEETVDEEENYDTGDASLDNIRNQDEIGEQELLVLSFGTSYNDSRRLTVGAIENDLESAFPDFSVRRGFTSNIIIDHVNKRDNVLIDDVDAAMNRAVDNGVKTLVVQPTHLMHGLEYDELVKEVGNYADAFDQVVFGEPLLSSDDDFAKVEKAITEWTADYDDGKTAICFMGHGTEADSNEVYQKMQDLLTKDGYTNYFVGTVEATPSLEDVLAKVQAGDYERVVLEPLMVVAGDHANNDMAGDEDGSWKKTFEDAGYQVECLVRGLGENEAIRQIYVEHAQAAIDSIAKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0G6</accession>
    <name>R5V0G6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GCN5-related N-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01804</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000186">
        <property type="protein sequence ID" value="CCZ84078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01804"/>
    <dbReference type="EnsemblBacteria" id="CCZ84078">
        <property type="protein sequence ID" value="CCZ84078"/>
        <property type="gene ID" value="BN566_01804"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="206" mass="23581" checksum="85FF71C2AABEC3AA" modified="2013-07-24" version="1">MKLKRISEASMQEIVKAFSEYQYEAQEEGLYYLCKGKSGTEAYMKGFALAGIKSGWLHTISEKEEGYIMISEPTSKVCFGGLKDLILGIVKGMGLKGSIQFFKDMKASGTSLESKLKEAHQDFVQIEMLVVKRAYQHQGYMRKLVQIAFDRADALGVPCIVSTDGILKMKKYEHIGFEHVQDRKFKEKVYEHDLIRYPKNKNQEQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0W9</accession>
    <name>R5V0W9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_00017</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000197">
        <property type="protein sequence ID" value="CCZ84248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00017"/>
    <dbReference type="EnsemblBacteria" id="CCZ84248">
        <property type="protein sequence ID" value="CCZ84248"/>
        <property type="gene ID" value="BN566_00017"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032688">
        <property type="entry name" value="ABC2_membrane_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12679">
        <property type="entry name" value="ABC2_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="80"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="103"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="156"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="226"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ84248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="250" mass="27903" checksum="5B8616F553706BD1" modified="2013-07-24" version="1">MGAIYRRELGSFFNSAIGPVFLTVFYLGSGVLFWLFCLNYARTDMSNYFAWMRLVMVLILPLLTMRLLSEERAQKTDQGLLTAPVSLGGIVLGKYFAALTVYAMGIAIVFVYAILLSFFGTVAWSIVFSNFIALFLMGAAFMAITLFLSAFTANQFIAYVLGIVSLVVLYMIDYLSGTVSSSADSLSSKSTVLSTLVGWLGNAMESVSFFSRIYDFTYGLFNLSSVLLYISTAVLFNFFTVRVFETRRWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5V142</accession>
    <name>R5V142_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cellulase M and related proteins</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN566_01709</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="8">Binds 2 divalent metal cations per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the peptidase M42 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000178">
        <property type="protein sequence ID" value="CCZ83980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01709"/>
    <dbReference type="EnsemblBacteria" id="CCZ83980">
        <property type="protein sequence ID" value="CCZ83980"/>
        <property type="gene ID" value="BN566_01709"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008007">
        <property type="entry name" value="Peptidase_M42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023367">
        <property type="entry name" value="Peptidase_M42_dom2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05343">
        <property type="entry name" value="Peptidase_M42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001123">
        <property type="entry name" value="PepA_GA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="202"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="313"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR001123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001123-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001123-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ83980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="342" mass="36876" checksum="ADA73360CBD50311" modified="2013-07-24" version="1">MLNHLESLCRLCGASGQEHPVRDYLIEQIKPLGLPYQVDPLGNLIVEKKGKQTTPKKWMIGAHMDEVGLIVTYINENGTMKLAPVGGIDPSVVIGRAVQVGEQRIPGVIGAKPIHLLSREDRKKAPSFSNLYLDIGAANREEAEQFVQPGTYAHFRSGYTLLGGGKLVRSKALDDRIGCAILLDLLQKDVPYDFTAAFHVQEEIGLRGAKAAAYTINPDFALILETTTAADFSSAQGDAKVCQLGKGPVVSFMDRSTLYDRELYRLAFSLCKEAGIPCQTKTRIAGGNDSGAVHVSRNGVRTLAISAPCRNLHSPSCVANLSDIQHCATLAALLLQQAGSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>R5V183</accession>
    <name>R5V183_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Stage 0 sporulation protein A homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN566_00261</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000217">
        <property type="protein sequence ID" value="CCZ84348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00261"/>
    <dbReference type="EnsemblBacteria" id="CCZ84348">
        <property type="protein sequence ID" value="CCZ84348"/>
        <property type="gene ID" value="BN566_00261"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Response regulatory" evidence="9">
        <location>
            <begin position="3"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="10">
        <location>
            <begin position="129"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="8">
        <location>
            <begin position="129"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="7">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ84348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="228" mass="25948" checksum="D7E63FA239906A9C" modified="2013-07-24" version="1">MSHICIIEDDTAIAEIERDYLEINGFTVSIATDGVSGLNLAQTESFDLILLDLMLPQLDGFTLCRKLRERLDIPILMVTAKKEDIDKIRGLGLGADDYIVKPFSPGELVARVQAHIATYKRLKTPQETQQEITVGAIRICPKSHRVFVREQEVSLKNKEYELLLFLVTNADTVFDRETLYERIWGMDAMGDNATVAVHINRLREKLEEDPSHPKYIETVWGAGYRFHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5V196</accession>
    <name>R5V196_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00039</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000219">
        <property type="protein sequence ID" value="CCZ84358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84358">
        <property type="protein sequence ID" value="CCZ84358"/>
        <property type="gene ID" value="BN566_00039"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046237">
        <property type="entry name" value="DUF6270"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19786">
        <property type="entry name" value="DUF6270"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="443" mass="51959" checksum="E9F0A9398D01951A" modified="2013-07-24" version="1">MENKQQPVVNICGSCILRDTFYYQGVYGKEKDGGYRINRYVNFFSPLALEQPPIPIYDRAAYEAFDLKPFGLTNFFRRCRRLDFERSFMEYLNDPADFLLVDTVMFRFQPYQMQVNGQKLICHGEHRPALEAMVKQGILPQLDAEVFSFESLSEEELRHRLQVYANQLKTLCPEERIILVETRHVFLQIDHANHVKSFSSTSQKIYRDGNRRMQKVFGILRELLPQAHLIPFPKQVLAETEHRLGVSGLHYTREYYEYCFQAVETIRKGLPRETEQQQIQALCDACTMQYTETYAGYIEESFAAVDVKKNQLQAERDRFLKYADFFRLYLEQHADVVRFCYKKQIRTIGLYAQNRITLYLRPILEEAGIHVRFIVENLPKSEQKKMKDFPQPFTLLSRSAEQYPETDAVLVADVMSPDTIAAACRKRTTAPVYTVYDLLEKKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1C5</accession>
    <name>R5V1C5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01766</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000181">
        <property type="protein sequence ID" value="CCZ84040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84040">
        <property type="protein sequence ID" value="CCZ84040"/>
        <property type="gene ID" value="BN566_01766"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="89" mass="10589" checksum="24A5830C28BBF9CA" modified="2013-07-24" version="1">MDKPRVSKIFDIPPYYYFESGNDYSGSKNLNQFNYKVEYGKENLTLKIWHGMLCSEKATIEIEQTYPNNAEGFQEMIHFLEETYQKEQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1F0</accession>
    <name>R5V1F0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Acetyltransferase GNAT family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00310</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000224">
        <property type="protein sequence ID" value="CCZ84403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00310"/>
    <dbReference type="EnsemblBacteria" id="CCZ84403">
        <property type="protein sequence ID" value="CCZ84403"/>
        <property type="gene ID" value="BN566_00310"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="151" mass="17388" checksum="3EE18EFB8DE99414" modified="2013-07-24" version="1">MTIRKLTAADEPIFYEMCEEFFHSSAVCHPIPMAHHVATFQELLQADTYLFGYLMEVDGEPAGFAILNQMMQQEAGGIIIWVECLYVREAFRSQGIGRQFLQFVAAEWKGKAKQLRLEVEPDNVRAKALYERMGYKVLPYQEMILSLDEAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1P5</accession>
    <name>R5V1P5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Lysophospholipase L1 and related esterases</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01930</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84205">
        <property type="protein sequence ID" value="CCZ84205"/>
        <property type="gene ID" value="BN566_01930"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002105">
        <property type="entry name" value="Dockerin_1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00404">
        <property type="entry name" value="Dockerin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004394930" evidence="1">
        <location>
            <begin position="31"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="426" mass="47536" checksum="8E8CE957AD550643" modified="2013-07-24" version="1">MTARKRILQLGLGVAAAALLAAVQPCSASAQDVVLFPETIQVERYTGTFQKEFHIDIDSDGEKETIGYYYVGSKRDGYSISDVYSVFDTNSESYTLYKRISNDHMNYKFHIIQDKNTGETFLGYIYTDMFGSEMLYRFYSDNQSKLISSYNANTDGSDKLQAYKTYMNNVDFLDEMAYGHGDVDGDADVTVSDAVATLSYYAATAAGQSPSFTELQKQAADFNQDGIVTVEDAVKTLSLYAEHAAGMVDTVLLPTTIRKDSSPFSLYGGTLESTFYLDIDGDGKEETIGRYDHIKGRHELCSDCIFQVYDDGTFQGSFGGNTDSQIFSHTMLAYDHNIDKIYLADCVGHLILGCGSPGLQNLYPNYGKYIIYIEATGMKPYAGINGKDATKQEIYDYLDQIEIISSDSNDEQLLEQFKELIIDRYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5V205</accession>
    <name>R5V205_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Oxidoreductase aldo/keto reductase family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00258</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000217">
        <property type="protein sequence ID" value="CCZ84345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00258"/>
    <dbReference type="EnsemblBacteria" id="CCZ84345">
        <property type="protein sequence ID" value="CCZ84345"/>
        <property type="gene ID" value="BN566_00258"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NADP_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="SSF51430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldo_ket_red" evidence="1">
        <location>
            <begin position="2"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="227" mass="25638" checksum="D81675B475841FC9" modified="2013-07-24" version="1">MQRLGTDYLDLYIIHRFDYETPIEETMEALNDLVKVGKVRALGASAMYGYQFYNMQLAARDNGWTPFQAMENHYNLLYREDERELIPICKQMGVTLMPYSPLAAGHLARPTWTANTCRSKTDRVAMGKYDRTEEQDMQIVRRVAEVAEHYGVKMQQIALAWQWAKGVAAPIVGATKSSYLDDAASALSVQLTDADIALLEECYVPHRIVGAIDKNPAEGVVLLDEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5V218</accession>
    <name>R5V218_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase FkbM family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00439</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000247">
        <property type="protein sequence ID" value="CCZ84578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00439"/>
    <dbReference type="EnsemblBacteria" id="CCZ84578">
        <property type="protein sequence ID" value="CCZ84578"/>
        <property type="gene ID" value="BN566_00439"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006342">
        <property type="entry name" value="FkbM_mtfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05050">
        <property type="entry name" value="Methyltransf_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01444">
        <property type="entry name" value="fkbM_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_21" evidence="1">
        <location>
            <begin position="185"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="357" mass="40579" checksum="79D5816FA09C6176" modified="2013-07-24" version="1">MLSFITEKLTAWEKLKAETRPIFIYGMGDGAEKIMRVFQEKGIPFAGIFASDDFVRGHSFAGHKVRKLSEIEAAVDDFVIVLAFAAGYQSLVDKIVDISKRHTLLVPDVPVAGGGLFTYDYCLEHADEIETVYNLLADDYSREVYASILNFKISGEIHYLMDVTTPKSEIYRKLMHLTPNEIYVDLGAYNGDTIREVLQYTRGKYIRIYAMEPDRKNFKKLLKNTDGMQNVYPFHAAAWCTDTQVPFATKAGRQSAVTATGQTIDARTVDSVLEGRPATLIKMDVEGCEREALWGSAQTIAHYTPKLMVSLYHRNEDMFSLPLLIQKLNPNYDIYIRHQLYIPAWETNLYAVPKKQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5V279</accession>
    <name>R5V279_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Fe2+/Zn2+ uptake regulation proteins</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00496</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Fur family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000257">
        <property type="protein sequence ID" value="CCZ84643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00496"/>
    <dbReference type="EnsemblBacteria" id="CCZ84643">
        <property type="protein sequence ID" value="CCZ84643"/>
        <property type="gene ID" value="BN566_00496"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07153">
        <property type="entry name" value="Fur_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002481">
        <property type="entry name" value="FUR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043135">
        <property type="entry name" value="Fur_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33202">
        <property type="entry name" value="PTHR33202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01475">
        <property type="entry name" value="FUR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="133" mass="15365" checksum="A4CE9873F2BFF2E2" modified="2013-07-24" version="1">MKNRNTLQRRLVLETVRRMRNHPTAEEIYLAIAAENPLISKATVYRNLKLLSEQGESLRIPIPDGADRFDFHVAPHYHIKCRVCSQVFDVDMPYQGNLCDQVTDAQGFQIESHQILFIGVCPDCQKKEKEQKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>R5V3U3</accession>
    <name>R5V3U3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Single-stranded-DNA-specific exonuclease RecJ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN566_00574</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RecJ family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000007">
        <property type="protein sequence ID" value="CCZ82769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00574"/>
    <dbReference type="EnsemblBacteria" id="CCZ82769">
        <property type="protein sequence ID" value="CCZ82769"/>
        <property type="gene ID" value="BN566_00574"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008409">
        <property type="term" value="F:5'-3' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004610">
        <property type="entry name" value="RecJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041122">
        <property type="entry name" value="RecJ_OB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17768">
        <property type="entry name" value="RecJ_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="SSF64182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00644">
        <property type="entry name" value="recJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHH" evidence="6">
        <location>
            <begin position="76"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="DHHA1" evidence="7">
        <location>
            <begin position="312"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="RecJ_OB" evidence="8">
        <location>
            <begin position="450"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ82769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="679" mass="74497" checksum="895C8E0CD9E15E45" modified="2013-07-24" version="1">MKKWQIQQPDEAAAKNIQANSDLSLFCAELLTVRGYRTIEETASQFSCPALSDPFLIRDMQAAADCLNAAIDSGEKICVYGDYDCDGVMATVILYSYLMELGADVVYYIPERSEGYGMNLNALETLKAQGVSLIVTVDNGIAALEEADWIAEQGMRLVITDHHQPYSTLPAAVAVVDPHRVDCPSPFSLFCGAGLALKLVAAMDGGDMTFALEQFGDLAAIATVADVVSLTGENRFLVQQGLRLLANTERPGLLALLELTGMQGKTLTATNLAFTLAPRINAAGRFGSPKLAVSLLLAETMEEAMPLAKQLEQCNRQRKEAEADILQEIQEQIQVHPELVQQRVLLFSGKDWHHGVIGIVAARLEEQFGKPVMVLTIEGEQARGSARAFGDFSVFACLDACRSHLTKYGGHPGAGGFSLSAAEIPAFFAAVQQYAAEQFPHMPQMTLQADRLLLPQDLTIENIASLSVLEPYGEGNPAPIFAICHAVLEAIQPLSGGLHSKLKLRYGSLYLDVLLFRVAPDAVGLPLQSMCDFLVTAECKTFQGRPQLSLIVKDYRRSGISQAKYFAAREAYETVCRKEPLPDAYYAAMTPTRSELVQVYRHLPETPLAMDTLFIQLQSQYNYGKLRLILDIFAELKLIQMDVWKETVQRLPVTGTVQLEQSAILAEIQEKTGRVSYDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5V4B6</accession>
    <name>R5V4B6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Flavin reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00754</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000032">
        <property type="protein sequence ID" value="CCZ82954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00754"/>
    <dbReference type="EnsemblBacteria" id="CCZ82954">
        <property type="protein sequence ID" value="CCZ82954"/>
        <property type="gene ID" value="BN566_00754"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ82954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="43" mass="5024" checksum="58B995DE9ECA4329" modified="2013-07-24" version="1">MSRNLIIYAFRKCENYVNGRIEHLSKGNSEQIVAKWAKEGIQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5V4Y3</accession>
    <name>R5V4Y3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Selenocysteine lyase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00957</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000069">
        <property type="protein sequence ID" value="CCZ83174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00957"/>
    <dbReference type="EnsemblBacteria" id="CCZ83174">
        <property type="protein sequence ID" value="CCZ83174"/>
        <property type="gene ID" value="BN566_00957"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010969">
        <property type="entry name" value="Cys_dSase-rel_unknwn_funct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01977">
        <property type="entry name" value="am_tr_V_EF2568"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotran_5" evidence="1">
        <location>
            <begin position="2"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="373" mass="39878" checksum="8C684A4019F0C5B6" modified="2013-07-24" version="1">MINFDNAATTFPKPRSVRQAMEMAMLKYGGNAGRGGHQLTERTSEQVFAAREEAAAFFGGKPENTIFCLNCTHALNLAIQGIVQPNDHVIISNLEHNAVLRPVAAMVRERGVQCSIAPVSVDDKETIAAFRARIRPNTRAIICTLASNVTGQILPYRELAALCNEHDICMIADGAQACGTLPVQMSDGINILCTAGHKGLYGATGTGLLITDTKFPIKPLLYGGTGSLSAKLEMPDFLPDRLECGTLNIAGILSLRAGIAFVKKTGTAAIFQHETACCNKLLSMLQTMPQVTVYRQPGAAYAPIVSFNIQNLSSEETAELLDQKGFCLRAGLHCAPLAHRAIGTIDGGTVRFAPSIFNTLPEVVALGNAIRNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5V4Z9</accession>
    <name>R5V4Z9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00977</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000070">
        <property type="protein sequence ID" value="CCZ83194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83194">
        <property type="protein sequence ID" value="CCZ83194"/>
        <property type="gene ID" value="BN566_00977"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004406759" evidence="2">
        <location>
            <begin position="27"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="86" mass="9216" checksum="A653A469D1EF5B75" modified="2013-07-24" version="1">MKQRMLAWLCAGILGLGCWGLPAAAADDTTEPTTQVTTEAETETESSKFSTKDVVTAVIVFTLVTAGTATGVFVFRWKKLKSSSQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5V542</accession>
    <name>R5V542_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01025</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000081">
        <property type="protein sequence ID" value="CCZ83244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01025"/>
    <dbReference type="EnsemblBacteria" id="CCZ83244">
        <property type="protein sequence ID" value="CCZ83244"/>
        <property type="gene ID" value="BN566_01025"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043740">
        <property type="entry name" value="DUF5685"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18937">
        <property type="entry name" value="DUF5685"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="293" mass="34119" checksum="F0892FA624B1BF1E" modified="2013-07-24" version="1">MYGYVQVYRPDLRFREYEVYRSHYCGLCHTLQQRCNAISRLTLSYDFTFLSLLLDALYEPETDTHKQRCFCGIGKNATVYHSAVLDYVADMHLFFAYLHCKDDWNDEYSILHAMLAAGIKAAYRRICNAYPQKTAAILEHMQQLTALEQQQETQFALPAACFGRALAESFCMEQSDVWNADLRHMGYHLGTFIYVLDAYDDLQKDRKHHTYNPFQQASIHDPAFHQTVKDFLQHAASEAAAAFERLPILQNADLLRNLLYAGIWQPFLKKQKEYQTAVNEQQNQESSFYVKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5V5E7</accession>
    <name>R5V5E7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01135</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000092">
        <property type="protein sequence ID" value="CCZ83359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83359">
        <property type="protein sequence ID" value="CCZ83359"/>
        <property type="gene ID" value="BN566_01135"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004395056" evidence="1">
        <location>
            <begin position="24"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="143" mass="16904" checksum="A39A079F6FE5BD58" modified="2013-07-24" version="1">MKKIITTVFCTAIALCSGLNVYAAEILERDYIEAEIWEEMWNGKGDNGLDFPEASYKHHLLDKWLDENYGSEDYDWTEIGELQYEYKDYYRHLIEDWDFEDDNEGGWTINTEDNHYSFFLLNGMWQMTDKNGDTVDSFPPSAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5V5F8</accession>
    <name>R5V5F8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01145</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000093">
        <property type="protein sequence ID" value="CCZ83369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01145"/>
    <dbReference type="EnsemblBacteria" id="CCZ83369">
        <property type="protein sequence ID" value="CCZ83369"/>
        <property type="gene ID" value="BN566_01145"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026881">
        <property type="entry name" value="WYL_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13280">
        <property type="entry name" value="WYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WYL" evidence="1">
        <location>
            <begin position="139"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="325" mass="39171" checksum="E9312EABA34A5FE5" modified="2013-07-24" version="1">MDHERIIYIYDYLSRYTDENHYVSISQIQEHLANVCNLTRVKNVTIRRDLDRLTAMGNHIVKHNREHNTAYYALIDKSFSFNEIRFIVDSISINKFLTAFQKKTLIKKFEGMCSDAEIRQLISRIVLNERCPSTLDLLENLDKIHRLIGERQKIDFEYGKYDETTKQIAYYYKKREMLPVSVIYFHERFYLKCLNEKDRKWRIYRVDRMKNILGGEVSYVRLPPEDNYEGFVTDMFAPEYFTVVTLRVKKYLLDEMRENFGDYASIFHDEDSDYVRIRVRVGINRKFYLWLMQYGDGVELVSPVKERAEYLEEVRKMLRAYPEFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5V5Y9</accession>
    <name>R5V5Y9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CobW/P47K family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01278</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000119">
        <property type="protein sequence ID" value="CCZ83509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83509">
        <property type="protein sequence ID" value="CCZ83509"/>
        <property type="gene ID" value="BN566_01278"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011629">
        <property type="entry name" value="Cbl_biosynth_CobW-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003495">
        <property type="entry name" value="CobW/HypB/UreG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02492">
        <property type="entry name" value="cobW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07683">
        <property type="entry name" value="CobW_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="cobW" evidence="1">
        <location>
            <begin position="6"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="CobW C-terminal" evidence="2">
        <location>
            <begin position="249"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="328" mass="37127" checksum="EEDB1002698A9484" modified="2013-07-24" version="1">MTTIDLITGILGAGKTTFIRKYATNLLAQGKKIAILENDFGAVNVDRMMLQDLQSDRCKVEMISGCNDPTCHKRRFKTQLIALGMQQFDRVILEPSGIFDMDEFFDTLYESPLDRWFSIGSILTIVDATMDEVLSAQMEYLLASEAACCGKLVLSKRSLLPDCSEAELTDRTLRHLNRSLAAIQCSRQFQPQDLFIKDWDSMTDADFQELQAAGYRNSSYVKQYDADDIQSHSEVHYFMHIHLPDERIPEILQAMLNDPNCGTIYRIKGALPLKQNCWLSINLTQKKAELQTVSNGQSVLIVIGDQLNREVLDAHLKAENTDPEYVSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>R5V633</accession>
    <name>R5V633_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA dimethylallyltransferase</fullName>
            <ecNumber evidence="6">2.5.1.75</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Dimethylallyl diphosphate:tRNA dimethylallyltransferase</fullName>
            <shortName evidence="6">DMAPP:tRNA dimethylallyltransferase</shortName>
            <shortName evidence="6">DMATase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Isopentenyl-diphosphate:tRNA isopentenyltransferase</fullName>
            <shortName evidence="6">IPP transferase</shortName>
            <shortName evidence="6">IPPT</shortName>
            <shortName evidence="6">IPTase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">miaA</name>
        <name evidence="10" type="ORF">BN566_01332</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 6 8">Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6 7">
            <text>adenosine(37) in tRNA + dimethylallyl diphosphate = diphosphate + N(6)-dimethylallyladenosine(37) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:26482"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10375"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74415"/>
            <dbReference type="EC" id="2.5.1.75"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="6">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 6 9">Belongs to the IPP transferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.75" evidence="6"/>
    <dbReference type="EMBL" id="CBAR010000131">
        <property type="protein sequence ID" value="CCZ83564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01332"/>
    <dbReference type="EnsemblBacteria" id="CCZ83564">
        <property type="protein sequence ID" value="CCZ83564"/>
        <property type="gene ID" value="BN566_01332"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052381">
        <property type="term" value="F:tRNA dimethylallyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00185">
        <property type="entry name" value="IPP_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039657">
        <property type="entry name" value="Dimethylallyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018022">
        <property type="entry name" value="IPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11088">
        <property type="entry name" value="PTHR11088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00174">
        <property type="entry name" value="miaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 6 7" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="16"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with substrate tRNA" evidence="6">
        <location>
            <begin position="39"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="14"/>
            <end position="21"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with substrate tRNA" evidence="6">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with substrate tRNA" evidence="6">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ83564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="329" mass="36913" checksum="6DA3848EE085A752" modified="2013-07-24" version="1">MQTKEKIPILAVVGPTASGKTALGVELAKAYNGEVVSADSMQIYKGMDIATAKPTPEEMQGIPHHLIDILPRDTIFNVADYVKLAEQAIADITQRGKLPILVGGTGLYLASLLDNVQFAETKQDAALRKELTDYAETHGTEALHERLKAVDPAAAAVIHPNNVVRVVRALEVTLLSGKPFSVQKAESRTQPSPYDACIIGLDYEDRQVLYDRINRRVDLMVENGLVEESYAVWKTNPKQTAANAIGYKELIPYFTGESDLSACIDKLKQETRRYAKRQLTWFRKRADVVWIKLYKFNNNKEILEKSKKIIAKQQFLCYNRIGLFETTEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5V670</accession>
    <name>R5V670_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00141</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000146">
        <property type="protein sequence ID" value="CCZ83614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83614">
        <property type="protein sequence ID" value="CCZ83614"/>
        <property type="gene ID" value="BN566_00141"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="46" mass="5402" checksum="93ED1AF52F694756" modified="2013-07-24" version="1">MAGCDSVPEVFGRFYLVRRLNIKITDKVKVRCLEHRTPHNLYLKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5V682</accession>
    <name>R5V682_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UDP-glucose pyrophosphorylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00156</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDPGP type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000146">
        <property type="protein sequence ID" value="CCZ83629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00156"/>
    <dbReference type="EnsemblBacteria" id="CCZ83629">
        <property type="protein sequence ID" value="CCZ83629"/>
        <property type="gene ID" value="BN566_00156"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070569">
        <property type="term" value="F:uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039741">
        <property type="entry name" value="UDP-sugar_pyrophosphorylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002618">
        <property type="entry name" value="UDPGP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11952">
        <property type="entry name" value="PTHR11952"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01704">
        <property type="entry name" value="UDPGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="407" mass="44977" checksum="1643FDF062A0DE17" modified="2013-07-24" version="1">MQYQEAYNRLEQYGQTHLLRYYDTLSAAEQAALLQQISEMDFSVLERAAHEEAPKGKIEPLGACTISEIAEKQDTYRKIGLEAIRKGSVGAVLLAGGQGSRLGCNGPKGVFQIGINNDLTIFECLFRNLMDVTEQAGVPVPLFIMTSETNDAETRAFLAEKQYFGYPESSVHFFVQEMAPVVDKDGKILLETKSRIAVSPNGNGGWFSSMQHAGLLNVLKEQNIEWLNVFAVDNVLQRMADPCFIGATIAANCTSGSKVVAKASPDEKVGVLCLEDGKPSIVEYFEMTDDMRNLREADGTLTYRYGVILNYLFRVDQLCKTLDCSLPLHRAFKKVACLTADGTATVKPEQPNGYKLETLVLDMVHMQENCLLYEVEREKEFAPVKNATGVDSVDTARALLKQNGVAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5V6A5</accession>
    <name>R5V6A5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01390</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000147">
        <property type="protein sequence ID" value="CCZ83654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83654">
        <property type="protein sequence ID" value="CCZ83654"/>
        <property type="gene ID" value="BN566_01390"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="132" mass="14818" checksum="6A3FF4E333C998E6" modified="2013-07-24" version="1">MQAMILGGIAAVICWAAWQRHNRTLDEQAADSGRELKPVPTGFDYQAAREQTDRMESNLKQYEQCNQLINDSVVAIKTGEGMPIEITHFDNRGRQVHTTLTDIPPEIVNDFARRLLDVCAERCGNTPPPPEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>R5V6M5</accession>
    <name>R5V6M5_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">30S ribosomal protein S21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpsU</name>
        <name evidence="7" type="ORF">BN566_01504</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the bacterial ribosomal protein bS21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000161">
        <property type="protein sequence ID" value="CCZ83769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01504"/>
    <dbReference type="EnsemblBacteria" id="CCZ83769">
        <property type="protein sequence ID" value="CCZ83769"/>
        <property type="gene ID" value="BN566_01504"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00358">
        <property type="entry name" value="Ribosomal_S21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001911">
        <property type="entry name" value="Ribosomal_S21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018278">
        <property type="entry name" value="Ribosomal_S21_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038380">
        <property type="entry name" value="S21_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21109">
        <property type="entry name" value="PTHR21109"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01165">
        <property type="entry name" value="Ribosomal_S21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00976">
        <property type="entry name" value="RIBOSOMALS21"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00030">
        <property type="entry name" value="S21p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01181">
        <property type="entry name" value="RIBOSOMAL_S21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ83769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="57" mass="6692" checksum="F6A266C8D2F8617A" modified="2013-07-24" version="1">MAEVRVGKNESLDTALKRFKRSCAKDGVIAEVRKREHYEKPSVKRKKKSEAARKRKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>R5V6M9</accession>
    <name>R5V6M9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Elongation factor P</fullName>
            <shortName evidence="8">EF-P</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">efp</name>
        <name evidence="12" type="ORF">BN566_01509</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 8">Involved in peptide bond synthesis. Stimulates efficient translation and peptide-bond synthesis on native or reconstituted 70S ribosomes in vitro. Probably functions indirectly by altering the affinity of the ribosome for aminoacyl-tRNA, thus increasing their reactivity as acceptors for peptidyl transferase.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Protein biosynthesis; polypeptide chain elongation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8 9">Belongs to the elongation factor P family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000161">
        <property type="protein sequence ID" value="CCZ83774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01509"/>
    <dbReference type="EnsemblBacteria" id="CCZ83774">
        <property type="protein sequence ID" value="CCZ83774"/>
        <property type="gene ID" value="BN566_01509"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00345"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04470">
        <property type="entry name" value="S1_EF-P_repeat_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05794">
        <property type="entry name" value="S1_EF-P_repeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00141">
        <property type="entry name" value="EF_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015365">
        <property type="entry name" value="Elong-fact-P_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020599">
        <property type="entry name" value="Transl_elong_fac_P/YeiP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013185">
        <property type="entry name" value="Transl_elong_KOW-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001059">
        <property type="entry name" value="Transl_elong_P/YeiP_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013852">
        <property type="entry name" value="Transl_elong_P/YeiP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011768">
        <property type="entry name" value="Transl_elongation_fac_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30053">
        <property type="entry name" value="PTHR30053"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01132">
        <property type="entry name" value="EFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08207">
        <property type="entry name" value="EFP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09285">
        <property type="entry name" value="Elong-fact-P_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005901">
        <property type="entry name" value="EF-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01185">
        <property type="entry name" value="EFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00841">
        <property type="entry name" value="Elong-fact-P_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00038">
        <property type="entry name" value="efp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01275">
        <property type="entry name" value="EFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8 12" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="6 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EFP" evidence="11">
        <location>
            <begin position="67"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Elong-fact-P_C" evidence="10">
        <location>
            <begin position="129"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CCZ83774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="185" mass="20888" checksum="0359B64F275CC8AD" modified="2013-07-24" version="1">MISAGDFRNGVTFELDGNVVQVIEFQHVKPGKGAAFVRTKYKNVITGAVVERSFNPTDKYPTAYIERKDMQYLYEDGGLYYFMDMETYEQLPIDGKKLGDAFRFVKENMEVKVLSYKGNVFGVEPPFFVELEVTETDPGFKGDTATNATKPATLETGAEIKVPLFINTGDKIRIDTRTGEYMERA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>R5V6Q8</accession>
    <name>R5V6Q8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-type sugar transport systems permease components</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_01539</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000164">
        <property type="protein sequence ID" value="CCZ83804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01539"/>
    <dbReference type="EnsemblBacteria" id="CCZ83804">
        <property type="protein sequence ID" value="CCZ83804"/>
        <property type="gene ID" value="BN566_01539"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008643">
        <property type="term" value="P:carbohydrate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="17"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="121"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="231"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="284"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="84"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ83804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="341" mass="38511" checksum="8E67B0425A4ED90C" modified="2013-07-24" version="1">MASAAQRRIKSISYAKYGYFFIAPFFLVYAFFQIWPLINTFILSVYGNGSEGDTFVGLQNFQTLLFGGDDRSARRIHAEFVRDFGNTLILWIGNFIPQILLSLLLAVWFTDSKLHIPGQGFFKVVMYLPNIITAVSVAALFMRFFSNSNMCLVNGLLTSAGKDAFMFEDNAAWSRAIVMFIQTWLWFGNTMIMMMSGILGINPSLFEAAAIDGATSGQVLRKVTLPLLRPMVVYTLITSMIGGLQMFDIPFLFHTKRSTINKYLETAAVFVYKNFRTSEDKVQYGYAGAASIILFIITAILGIIVFRMNRDADEARKKKERKELVKAYKREQKLAKMGGVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7D1</accession>
    <name>R5V7D1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Membrane-associated zinc metalloprotease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BN566_01723</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MGS:254</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M50B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000178">
        <property type="protein sequence ID" value="CCZ83994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01723"/>
    <dbReference type="EnsemblBacteria" id="CCZ83994">
        <property type="protein sequence ID" value="CCZ83994"/>
        <property type="gene ID" value="BN566_01723"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041489">
        <property type="entry name" value="PDZ_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004387">
        <property type="entry name" value="Pept_M50_Zn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008915">
        <property type="entry name" value="Peptidase_M50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42837">
        <property type="entry name" value="PTHR42837"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17820">
        <property type="entry name" value="PDZ_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02163">
        <property type="entry name" value="Peptidase_M50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="SSF50156"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="12" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="271"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="317"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M50" evidence="10">
        <location>
            <begin position="10"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ_6" evidence="11">
        <location>
            <begin position="137"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CCZ83994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="344" mass="38081" checksum="E6471B99464D1E01" modified="2013-07-24" version="1">MTTIWSILLAILIFGCIIAIHEFGHFIAAKRNGIQVNEFAIGMGPKIFRFQKGETLYSLRLFPIGGFCSMEGEDAESKNARAFDQKPVWRRMIVVLAGAFMNLVLGFVLLIATTCSDPYLISNTIHGFHEQATSNTCGLQAGDTIVSLNGSHIFTITDLQYKLSNESGEPFTVVVERDGKKVKLQDVTFRDQENNDWLDFSLEPQQKTVFHVLSYAAKDTVATGKLIWMSLTELVTGKYGIQDLSGPVGTVEVISNAATAGATAAERISSVLHLTIFITINVGIFNLLPIPGLDGSRFWFLFLEAIRRKPVKKEHEALVHLLGMAALFLLMIVVTVQDVTRIFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7G7</accession>
    <name>R5V7G7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01745</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000180">
        <property type="protein sequence ID" value="CCZ84019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01745"/>
    <dbReference type="EnsemblBacteria" id="CCZ84019">
        <property type="protein sequence ID" value="CCZ84019"/>
        <property type="gene ID" value="BN566_01745"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039448">
        <property type="entry name" value="Beta_helix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002105">
        <property type="entry name" value="Dockerin_1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="GH16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13229">
        <property type="entry name" value="Beta_helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00404">
        <property type="entry name" value="Dockerin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00722">
        <property type="entry name" value="Glyco_hydro_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="SSF49899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="SSF51126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004394730" evidence="1">
        <location>
            <begin position="32"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="2">
        <location>
            <begin position="20"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="3">
        <location>
            <begin position="315"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="704" mass="78121" checksum="F830A0596D0F308F" modified="2013-07-24" version="1">MKKVLSILSAFAIVCTSTAFYSATNKISASAAEQTTDEFYDDFSSGTLDPDKWLVAYKNWGGKVTENGEKVDYNGGVIPQNVSVQNGKLVLTGNGNLYQGDLKGVNKDGSQRADGKRTGAAIATKDYYASGSYEVVAKVSPEFGACSAIWTFEYEEDWDTGAITNHEIDIEMPGRPNAEKTNQSYQYALCNTWIGENEGEYRTGYTDIGVNQADGAFHKYRFDWHTGDENEEARVEFYFDDVLVYTSKEYIPTNAGRLWLGLWFPNSWAGTPDFETSDFEIDSVKITPFHEAGDTAQNETYPEDGWGNLEDISHKSSVQGDVNADGVFDVADVVLLQKWLLTVPDTNLADWKAADFCEDDTLNVLDLCRMKQKLTSIESPTNQVYVKNTEELKAALENAKAGDEIILAEGEYVYSGDTPKGYMFTGTADGTEEKPIILRSENPDQPAILSGSSTAENYVLSISGYWWEIKDLKVTNAQKGIMIDNSNYTKIKNCEVYHIGSEGIHLRDNSSNCLIESCNVHDTGVVSPGYGEAIYVGSAESTTEYGHECHYNTIRNCKLGPNVAAEHVDIKEYTIGTTVENCTFDGTGMSGENYAKSFINIKGNDCIIRNNVGYRNGCTAIQRAFEQNNVVDGWGQNASVYGNQVYMDTATNALGKKMYFLNAWDCSATVWDNFMAYDGELFSVDHEDDHWNYYNCNLLTYGSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7L4</accession>
    <name>R5V7L4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Acetyltransferase</fullName>
            <ecNumber evidence="2">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01815</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CBAR010000186">
        <property type="protein sequence ID" value="CCZ84089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01815"/>
    <dbReference type="EnsemblBacteria" id="CCZ84089">
        <property type="protein sequence ID" value="CCZ84089"/>
        <property type="gene ID" value="BN566_01815"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016407">
        <property type="term" value="F:acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039369">
        <property type="entry name" value="LacA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024688">
        <property type="entry name" value="Mac_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43017">
        <property type="entry name" value="PTHR43017"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14602">
        <property type="entry name" value="Hexapep_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12464">
        <property type="entry name" value="Mac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01266">
        <property type="entry name" value="Mac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Mac" evidence="3">
        <location>
            <begin position="4"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU367021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="205" mass="22568" checksum="D0BB4A392F69DD1E" modified="2013-07-24" version="1">MTETEKMLAGKIYDASDSHLADRRIVAHRLSKQYNDTFEDEAEKRTAILKELIPNLGEDTYLQGPIQVDYGLYTTFGTRCYANFNFVVLDTCPITIGNNVFFGPNCTLATPLHPYLPEERNLREKEDGTLYTLEYGAPIHIGDNCWLAANVTVCGGVTIGAGCIIGAGSVVTRDIPAGSLAAGNPCRVIRPITEQDSIYLKKQLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7P7</accession>
    <name>R5V7P7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Beta-xylanase</fullName>
            <ecNumber evidence="6">3.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN566_00175</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Endohydrolysis of (1-&gt;4)-beta-D-xylosidic linkages in xylans.</text>
            <dbReference type="EC" id="3.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the glycosyl hydrolase 10 (cellulase F) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.8" evidence="6"/>
    <dbReference type="EMBL" id="CBAR010000190">
        <property type="protein sequence ID" value="CCZ84129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00175"/>
    <dbReference type="EnsemblBacteria" id="CCZ84129">
        <property type="protein sequence ID" value="CCZ84129"/>
        <property type="gene ID" value="BN566_00175"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031176">
        <property type="term" value="F:endo-1,4-beta-xylanase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045493">
        <property type="term" value="P:xylan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002105">
        <property type="entry name" value="Dockerin_1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044846">
        <property type="entry name" value="GH10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031158">
        <property type="entry name" value="GH10_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001000">
        <property type="entry name" value="GH10_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31490">
        <property type="entry name" value="PTHR31490"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00404">
        <property type="entry name" value="Dockerin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00331">
        <property type="entry name" value="Glyco_hydro_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00134">
        <property type="entry name" value="GLHYDRLASE10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00633">
        <property type="entry name" value="Glyco_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00591">
        <property type="entry name" value="GH10_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51760">
        <property type="entry name" value="GH10_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3 6 9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1 6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0858">Xylan degradation</keyword>
    <feature type="domain" description="GH10" evidence="7">
        <location>
            <begin position="1"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="8">
        <location>
            <begin position="251"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ84129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="314" mass="35344" checksum="7858877360F2EF24" modified="2013-07-24" version="1">MLKRMENYIKNVMEGLATQYPDVEFYAWDVVNEAWLDDGNPRKAGSSKSEDSNSSAWVRIFGDNSFIEYAFQYARKYAPKNCKLYYNDFNEYMPGKTTAIYNMAMELKEKGLIDGIGMQSHLDVGFPTASTYRKAIDKFASTGLDIQVTELDITTSDTSEAGLEKQAQLYSDIFDIYTDYADSISAVVVWGVTDDQSWRATRVPLLFDENFQAKPAYYSIVDNVEPKVTTTTTTEITTDTTPSETTTTTEPTFRLGDVNLDGNVNVQDVVLLQKYLIRKETLTAEQTKPADSNSDGVINVIDLSCLKKWILSVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5V823</accession>
    <name>R5V823_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00208</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000207">
        <property type="protein sequence ID" value="CCZ84279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84279">
        <property type="protein sequence ID" value="CCZ84279"/>
        <property type="gene ID" value="BN566_00208"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="161" mass="17964" checksum="E572944454265B20" modified="2013-07-24" version="1">MLKKILSRTTCAACQLCCQFDASDIWELPVLPAETVAAIRKYRPETAFVPVGAEQTFAAPPLKGEELYACPVLTEHGCGLSEQDKPFDCKVWPFRLMRTANGTVCISISDLCTGVQLHEVEQLQAFLREGLAETMFAYAKAHPDHIHAWHDGYRVILTEPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5V8A2</accession>
    <name>R5V8A2_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CRISPR-associated endonuclease Cas1</fullName>
            <ecNumber evidence="8">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">cas1</name>
        <name evidence="9" type="ORF">BN566_00267</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Acts as a dsDNA endonuclease. Involved in the integration of spacer DNA into the CRISPR cassette.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer, forms a heterotetramer with a Cas2 homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the CRISPR-associated endonuclease Cas1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="8"/>
    <dbReference type="EMBL" id="CBAR010000218">
        <property type="protein sequence ID" value="CCZ84354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00267"/>
    <dbReference type="EnsemblBacteria" id="CCZ84354">
        <property type="protein sequence ID" value="CCZ84354"/>
        <property type="gene ID" value="BN566_00267"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004520">
        <property type="term" value="F:endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043571">
        <property type="term" value="P:maintenance of CRISPR repeat elements"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09721">
        <property type="entry name" value="Cas1_I-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01470">
        <property type="entry name" value="Cas1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002729">
        <property type="entry name" value="CRISPR-assoc_Cas1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042206">
        <property type="entry name" value="CRISPR-assoc_Cas1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019856">
        <property type="entry name" value="CRISPR-assoc_Cas1_DVULG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042211">
        <property type="entry name" value="CRISPR-assoc_Cas1_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01867">
        <property type="entry name" value="Cas_Cas1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00287">
        <property type="entry name" value="cas1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03640">
        <property type="entry name" value="cas1_DVULG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 8 9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ84354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="343" mass="39460" checksum="88ABEE614ECDF898" modified="2013-07-24" version="1">MKQLRQTLYIMTPERYLSLDGENVVVQEKKTVLARLPLHNLDGIICFGATGASPPLMGKCAEMGLPISFLSRSGKFLCRMEGTTSGNVLLRRQQYRLADQVSERLKIAKSMITGKIFNSRYVLERAIRDHGLRMDVEKMQKKSEFLRAAAQRAWEASDLDSLRGIEGEAASVYFSVWDDLILQQKEHFYFSVRSRRPPLDRVNALLSFAYSFMTSMCTAALESVGLDPYIGFLHTDRPGRRSLALDLVEEFRAPLCDRFVLTLINRRMIQEKQFEKQADGAVWLNEEGRKLFLEAWQKKKAEVIPHPFLKEKVQYGMLPYAQALLLARYIRGDLDDYPAMMWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>R5V8I4</accession>
    <name>R5V8I4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ATP synthase epsilon chain</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">ATP synthase F1 sector epsilon subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">F-ATPase epsilon subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">atpC</name>
        <name evidence="10" type="ORF">BN566_00331</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 7">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the ATPase epsilon chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000225">
        <property type="protein sequence ID" value="CCZ84424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00331"/>
    <dbReference type="EnsemblBacteria" id="CCZ84424">
        <property type="protein sequence ID" value="CCZ84424"/>
        <property type="gene ID" value="BN566_00331"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045261">
        <property type="term" value="C:proton-transporting ATP synthase complex, catalytic core F(1)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12152">
        <property type="entry name" value="F1-ATPase_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00530">
        <property type="entry name" value="ATP_synth_epsil_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036794">
        <property type="entry name" value="ATP_F1_dsu/esu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001469">
        <property type="entry name" value="ATP_synth_F1_dsu/esu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020547">
        <property type="entry name" value="ATP_synth_F1_dsu/esu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020546">
        <property type="entry name" value="ATP_synth_F1_dsu/esu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036771">
        <property type="entry name" value="ATPsynth_dsu/esu_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13822">
        <property type="entry name" value="PTHR13822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00401">
        <property type="entry name" value="ATP-synt_DE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02823">
        <property type="entry name" value="ATP-synt_DE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46604">
        <property type="entry name" value="SSF46604"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51344">
        <property type="entry name" value="SSF51344"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01216">
        <property type="entry name" value="ATP_synt_epsi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6 7" id="KW-0139">CF(1)</keyword>
    <keyword evidence="6" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="3 6 7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATP-synt_DE_N" evidence="9">
        <location>
            <begin position="5"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-synt_DE" evidence="8">
        <location>
            <begin position="87"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ84424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="138" mass="15643" checksum="072A40533F23E247" modified="2013-07-24" version="1">MAKTFKLELLAADRPFFVGECEHLIYPAQDGLVGVLPGHESLVTIVSPGEIQYKVDGEWRYAAISEGFAEIRSDYALILGDAIELPEEIDAKRAAEAATRAKERLRQRQSIMQYYQSQAALNRAMNRLKISRRHNHEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>R5V8M4</accession>
    <name>R5V8M4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">S-adenosylmethionine synthase</fullName>
            <shortName evidence="11">AdoMet synthase</shortName>
            <ecNumber evidence="11">2.5.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">MAT</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Methionine adenosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">metK</name>
        <name evidence="17" type="ORF">BN566_00363</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>ATP + H2O + L-methionine = diphosphate + phosphate + S-adenosyl-L-methionine</text>
            <dbReference type="Rhea" id="RHEA:21080"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.5.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="11">Binds 1 potassium ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Binds 2 divalent ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homotetramer; dimer of dimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11 13">Belongs to the AdoMet synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.6" evidence="11"/>
    <dbReference type="EMBL" id="CBAR010000238">
        <property type="protein sequence ID" value="CCZ84464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00363"/>
    <dbReference type="EnsemblBacteria" id="CCZ84464">
        <property type="protein sequence ID" value="CCZ84464"/>
        <property type="gene ID" value="BN566_00363"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00315">
        <property type="reaction ID" value="UER00080"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004478">
        <property type="term" value="F:methionine adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006556">
        <property type="term" value="P:S-adenosylmethionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00086">
        <property type="entry name" value="S_AdoMet_synth1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022631">
        <property type="entry name" value="ADOMET_SYNTHASE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022630">
        <property type="entry name" value="S-AdoMet_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022629">
        <property type="entry name" value="S-AdoMet_synt_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022628">
        <property type="entry name" value="S-AdoMet_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002133">
        <property type="entry name" value="S-AdoMet_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022636">
        <property type="entry name" value="S-AdoMet_synthetase_sfam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11964">
        <property type="entry name" value="PTHR11964"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02773">
        <property type="entry name" value="S-AdoMet_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02772">
        <property type="entry name" value="S-AdoMet_synt_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00438">
        <property type="entry name" value="S-AdoMet_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000497">
        <property type="entry name" value="MAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55973">
        <property type="entry name" value="SSF55973"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01034">
        <property type="entry name" value="metK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00376">
        <property type="entry name" value="ADOMET_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00377">
        <property type="entry name" value="ADOMET_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 11 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 11" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="10 11 12" id="KW-0630">Potassium</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="S-AdoMet_synt_N" evidence="14">
        <location>
            <begin position="4"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="S-AdoMet_synt_M" evidence="15">
        <location>
            <begin position="120"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="S-AdoMet_synt_C" evidence="16">
        <location>
            <begin position="239"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Flexible loop" evidence="11">
        <location>
            <begin position="99"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="170"/>
            <end position="172"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="236"/>
            <end position="237"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="245"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="251"/>
            <end position="252"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CCZ84464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="391" mass="42566" checksum="A29D139EAB870823" modified="2013-07-24" version="1">MNRFLFTSESVTEGHPDKICDQISDAVLDAILEQDSKARVACETVVTTGMVLCMGEISTTAKVDIPQIARDVIVDIGYDRAKFGFDGYTCSVLQAMDKQSPDIAMGVNDAYESRGGEHQETGAGDQGMMFGYACKETKELMPLAISLAHKLCMKLTDVRKEKVLQYLRPDGKAQVTVEYVDGKPKRIDAVVLSTQHAPTVTLEQIRSDLMHYVITPVLPAEMLDENTKYYINPTGRFVVGGPQGDSGLTGRKIIVDTYGGSARHGGGAFSGKDPTKVDRSAAYAARYIAKNIVAADLADRCEVQLAYAIGVAHPVSVLVDTFGTGKVDEEKLSAAVQKVFSLTPDGIIEMLQLRKPQYRKLAAYGHMGREDLGVAWEKCDRVEALKQAVAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5V8X1</accession>
    <name>R5V8X1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00080</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000241">
        <property type="protein sequence ID" value="CCZ84534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00080"/>
    <dbReference type="EnsemblBacteria" id="CCZ84534">
        <property type="protein sequence ID" value="CCZ84534"/>
        <property type="gene ID" value="BN566_00080"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06088">
        <property type="entry name" value="KOW_RPL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041985">
        <property type="entry name" value="RPL14_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="80" mass="9099" checksum="1F13B1AD0F02844C" modified="2013-07-24" version="1">MEWQQGSIVLATAGRDAGMYFVVVAADEHFVWLANGKRRTLANPKRKNKKHVQQTATVIALTSWTDKRLRTWLNEFAAAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>R5V8Z7</accession>
    <name>R5V8Z7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GDP-mannose 4,6-dehydratase</fullName>
            <ecNumber evidence="3">4.2.1.47</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_00430</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAD(P)-dependent epimerase/dehydratase family. GDP-mannose 4,6-dehydratase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.47" evidence="3"/>
    <dbReference type="EMBL" id="CBAR010000247">
        <property type="protein sequence ID" value="CCZ84569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00430"/>
    <dbReference type="EnsemblBacteria" id="CCZ84569">
        <property type="protein sequence ID" value="CCZ84569"/>
        <property type="gene ID" value="BN566_00430"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008446">
        <property type="term" value="F:GDP-mannose 4,6-dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019673">
        <property type="term" value="P:GDP-mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006368">
        <property type="entry name" value="GDP_Man_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43715">
        <property type="entry name" value="PTHR43715"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01472">
        <property type="entry name" value="gmd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-bd_dom" evidence="4">
        <location>
            <begin position="1"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ84569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="283" mass="32232" checksum="FDD1F2963E9A0957" modified="2013-07-24" version="1">MTDEISLIRAMQISQADEVYNLAAQSFVATSWEQPMATAEIDALGVTKMLEAIKFVKPSCRFYQASTSEMFGLVQEMPQTEKTPFYPRSPYGVAKLYGHWITKNYRESFNLFACSGILFNHESERRGLEFVTRKITTAAARISLGLQDHVELGNLDAKRDWGHSKDYVRAMWLMLQQDAPDDYVIATNETRTVREFVEIAFAHVGIEIAWQGSGTDEIGVDRKTGKTVVTINPQFFRPAEVQVLLGNPAKAEEKLGWKREISFKELVERMVRHDLELAKKEVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5V930</accession>
    <name>R5V930_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">D-alanyl-D-alanine carboxypeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00462</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000257">
        <property type="protein sequence ID" value="CCZ84609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00462"/>
    <dbReference type="EnsemblBacteria" id="CCZ84609">
        <property type="protein sequence ID" value="CCZ84609"/>
        <property type="gene ID" value="BN566_00462"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1380.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009045">
        <property type="entry name" value="Hedgehog_sig/DD-Pept_Zn-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003709">
        <property type="entry name" value="Pept_M15B"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02557">
        <property type="entry name" value="VanY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55166">
        <property type="entry name" value="SSF55166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="68"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="VanY" evidence="3">
        <location>
            <begin position="192"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="404"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="404"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="420"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="445" mass="50079" checksum="4C4B9DC49414206D" modified="2013-07-24" version="1">MQEKKRKKKKSTAEQEEQTKKIQSVAETASEQPTAKELPKESVELPSKSNPTASGSANTKENRRTGWILAEVAISAALLVAMRLTLLHPFLDNWTEVNTMSGSTWLEDNAQQVPLTTTTPDSTDPTGTTDSNKNGNQSDLYKSVSMDNQEIHNGDLILVNNSTAYESTDENELEAVYEKKTDSYSVSGSELMLRGDVIENLNNMLDDFQTETGHDDIIIISGYRTTEQQQELYDADLEDTGEDTSTLVAKPGYSEHETGYAMDFSLFEDGISADYDGTGEYDWINQNCAHYGFILRYPENKTDLTEIRYESWHYRYVGQPHAYYMQQNDLCLEEYTETLKDYSVDNPLEITNWDGKVYQVYYVPAEAGDSTYVMVPPDQEYTVSGNNVDGFIITVDTGVTNLDEAETKTTTTTATSDGEDTETTETETETDALAETDWNTEDWEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5VGS4</accession>
    <name>R5VGS4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative ornithine cyclodeaminase mu-crystallin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00586</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000008">
        <property type="protein sequence ID" value="CCZ82781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00586"/>
    <dbReference type="EnsemblBacteria" id="CCZ82781">
        <property type="protein sequence ID" value="CCZ82781"/>
        <property type="gene ID" value="BN566_00586"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1780.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003462">
        <property type="entry name" value="ODC_Mu_crystall"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023401">
        <property type="entry name" value="ODC_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13812">
        <property type="entry name" value="PTHR13812"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02423">
        <property type="entry name" value="OCD_Mu_crystall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001439">
        <property type="entry name" value="CryM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ82781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="379" mass="41727" checksum="76C0D7A75899B98F" modified="2013-07-24" version="1">MSYPKVDILYLSEKDMLAAGVNNVVSCTDCMEEVVKLLDDGDYRMGGENGNSHGCMISFPDNPEFPNMPKNGPDRRFMAMPAYLGGKFDMAGVKWYGSNVENREKGLPRSILMVILNDKETGAPKAFLSANLISAYRTAAIPGVGVKLLAKKDAKVLGVVGPGFINNITIETFAALRPTLDTLKIKGRGRASIDRCIAYVKEHCPQFKNIIVCDTLEECCKDADIMTIAVSTPTGGHQNYPFIKREWLKPGCLVCAPGAVDFPDELVCDPTVKLVVDNKKLYEAWAEEYPYPTFDKMFLLGDKFTDLAHDGKLDWGIVQNIGAIANGKVPGRESDDQIILYSIGGMPVEDVAWGKTVYENALEKGIGVTLNLWDQPYLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VGW0</accession>
    <name>R5VGW0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00616</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000009">
        <property type="protein sequence ID" value="CCZ82811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82811">
        <property type="protein sequence ID" value="CCZ82811"/>
        <property type="gene ID" value="BN566_00616"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ82811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="187" mass="21645" checksum="8AC79195A685B7D5" modified="2013-07-24" version="1">MQTGTSVAKVLQFFTRKPYNHASVALDLTLEEMYSFCRNHIHFPLPAGFNQEIVGEGTLGRFLVIPCEIYAIPVTKEKKQEFEQNLAYFKNHRSMFSYNVLGLGTTFLRIRWSRKHKLHCSQFVATLLAQSGVRLNKPLSLHTPDDLRYISNAHLIYRGELNRFYHTYAKSIQQLEDSMPPMYSNPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5VH70</accession>
    <name>R5VH70_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00703</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000022">
        <property type="protein sequence ID" value="CCZ82901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00703"/>
    <dbReference type="EnsemblBacteria" id="CCZ82901">
        <property type="protein sequence ID" value="CCZ82901"/>
        <property type="gene ID" value="BN566_00703"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008998">
        <property type="term" value="F:ribonucleoside-triphosphate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012833">
        <property type="entry name" value="NrdD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13597">
        <property type="entry name" value="NRDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ82901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="92" mass="10517" checksum="E0B37A5FFAACE449" modified="2013-07-24" version="1">MTVYDSIPVTVTGGTLSDAEIAAYVKHSKKTYPNRKIEQLSIQVDGDFVDLRCQFAPENFERIRRITGYLVGTLDRFNDAKRAEVQDRVKHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5VHR4</accession>
    <name>R5VHR4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted nucleoside-diphosphate sugar epimerases</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00829</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the polysaccharide synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000040">
        <property type="protein sequence ID" value="CCZ83036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00829"/>
    <dbReference type="EnsemblBacteria" id="CCZ83036">
        <property type="protein sequence ID" value="CCZ83036"/>
        <property type="gene ID" value="BN566_00829"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05237">
        <property type="entry name" value="UDP_invert_4-6DH_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003869">
        <property type="entry name" value="Polysac_CapD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02719">
        <property type="entry name" value="Polysacc_synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Polysacc_synt_2" evidence="3">
        <location>
            <begin position="299"/>
            <end position="578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="638" mass="72305" checksum="93B25B96459F0B55" modified="2013-07-24" version="1">MLLKRYATRKKNSVRKYILMAADIGILFLCIVLTVFVFFLLERPVMQMNHLLPYFAINILLSFLTLTVSGAYDVFWRYFNMRDYITCSLGVVIGQVLSSLVVLLLRWHVPMGFLALNAILAIGGIVVFRLVFRHTFLILTDAGRVESRKRLLIVGAGSGCELILKEIQRSCADPEKTPPIPYDPICIVDDDPLKRNATVMGVRVAGTTEDIKPLCDKLKIDMIMFAIPSCDEENRRRILDLCSHAGRPVKIMPYLSQLLFQKDIIPQIQDIRIEDLLGREPAKFDREKVAGFLKDKVCMITGGGGSIGSEIVRQIMTYHPKKVIIVDIYENNAYDIQQELFLTYGSKIPLFVEIASVRDHAKLITLFEKYHPQIVFHAAAHKHVPLMESCPEEAVKNNIVGTFYLATLAATFQCEKFIMISTDKAVHPANVMGATKRCCEMIIQYMSQHSTNTEFITTRFGNVLGSNGSVIPLFRKQLESGKPLTVTHPKMIRYFMTIPEAVSLVLHASAMAHGGEIFVLDMGAPVRILTLAENLIAAYGKRPYFDVKIRFIGLRPGEKMEEELMTEQENKQQKTLQETENSQIFIGKQIAFDDSNFISRLYKIEQAALRNDREETLQLLKELVPDFHHEDANSFTAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5VI62</accession>
    <name>R5VI62_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ACT domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01027</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000081">
        <property type="protein sequence ID" value="CCZ83246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01027"/>
    <dbReference type="EnsemblBacteria" id="CCZ83246">
        <property type="protein sequence ID" value="CCZ83246"/>
        <property type="gene ID" value="BN566_01027"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008310">
        <property type="entry name" value="UPF0735_ACT_dom-cont"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13291">
        <property type="entry name" value="ACT_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF025624">
        <property type="entry name" value="ACT_PheB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACT" evidence="1">
        <location>
            <begin position="69"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="145" mass="15964" checksum="62219D0D82199625" modified="2013-07-24" version="1">MRAQFIVVNTSVLPEIFEKVLEVKKLVADHSEKSFASACKRVGISRSAFYKYRDYVFHYEDKMTQRIVNLTAVLRDEAGVLSSVLVELHNLHANILTVNQSIPVDGVAGVSLSLQIGSDSVDLTHITKTLSALHGVVEVKIISGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5VIB7</accession>
    <name>R5VIB7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01087</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83306">
        <property type="protein sequence ID" value="CCZ83306"/>
        <property type="gene ID" value="BN566_01087"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004395380" evidence="2">
        <location>
            <begin position="18"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="103"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="232" mass="26766" checksum="D3537A82800E7313" modified="2013-07-24" version="1">MGLFSCCFSQSTPKVGAESKPAIQKLKQWETTHFPAPRTSGSQNPAIRYQVYRTEQGSSISLKKMKVPAASSQSQMEFIFSKIKTITSGIDPNEVACWIFDYRTQISNEIQRFLENLQKLENLNKTLREDSLFFDIDHVFQVKVRNLYHELLFCKFENLGNAAYFQSVQSHLIAIRKDMNDLNTNFLDYMHALTNTEQEDTTQDLELIRMQVQAMAELAQKQIQDSYRGIGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJ81</accession>
    <name>R5VJ81_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Beta-glucosidase-related glycosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_01324</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000130">
        <property type="protein sequence ID" value="CCZ83556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01324"/>
    <dbReference type="EnsemblBacteria" id="CCZ83556">
        <property type="protein sequence ID" value="CCZ83556"/>
        <property type="gene ID" value="BN566_01324"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001764">
        <property type="entry name" value="Glyco_hydro_3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036962">
        <property type="entry name" value="Glyco_hydro_3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00933">
        <property type="entry name" value="Glyco_hydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004395384" evidence="3">
        <location>
            <begin position="23"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_hydro_3" evidence="4">
        <location>
            <begin position="95"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="434"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="437"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="453" mass="48463" checksum="C1352159C261736A" modified="2013-07-24" version="1">MKKKQFLWLMIAIGILSTGSGCDSVKPVSTTQEIPTAEETNLVTTVPISEGEQPAETTTVPQIADVLTTAATTPTTTGEEYEDTSLVELLMERMTLEQKVCQMFIVTPESLTGYNGAVTEAGALSKESFTAYPVGGLIYFSDNLEDAPQTVSMLSTMQDYAQETTGVGLFLSVDEEGGTVARVADNLGTTKLYDMEYYGERHNPEEAYAIGNTIGSDLIQFGFNVDFAPVADVNLNPNNELGSRIFSSDPDIVGDMVSGVVSGLQNMGVSATLKHFPGLGAGDGNTHYDDFVLIERSYSELKEEEFKAFQAGIDAGADFVMVGHQITTAAGDQLPSDMSYTVVTDWLKGTLGFDGIVITDAQNMAAITENYSPATAAVQSVSAGVDVILMPMDLSNAVSGICDAVENGTISEERINESVYKIMMKKLEMGLLTMPEEDPDSTTTSTTTTDIAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJI5</accession>
    <name>R5VJI5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Permeases</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_01432</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family. Azg-like subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000153">
        <property type="protein sequence ID" value="CCZ83696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01432"/>
    <dbReference type="EnsemblBacteria" id="CCZ83696">
        <property type="protein sequence ID" value="CCZ83696"/>
        <property type="gene ID" value="BN566_01432"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015205">
        <property type="term" value="F:nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904823">
        <property type="term" value="P:purine nucleobase transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045018">
        <property type="entry name" value="Azg-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026033">
        <property type="entry name" value="Azg-like_bact_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006043">
        <property type="entry name" value="NCS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43337">
        <property type="entry name" value="PTHR43337"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00860">
        <property type="entry name" value="Xan_ur_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005353">
        <property type="entry name" value="PbuG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="51"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="200"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="260"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="339"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="369"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="408"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="436"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="454" mass="48201" checksum="C18C6B7815B9905C" modified="2013-07-24" version="1">MIEKLFHLKENNTNIKTECIAGVTTFMTMAYILAVNPNILSAAGMNPTAVLLATCLASFLGTMCMGLMANLPFALSAGMGLNAFFAYTVVGNMGYPWQVALLAVFIEGLVFLVLSLTNVREALFNVIPMSLKQAVSVGIGVYIAFIGLQNAGVVVNNDSTLVSLVSFTDDFRTSGICALLAIIGIFLTAVLYIKGIKGSILIGILGTWVLGILCQLTGLYTVDAEAGYNSLIPTISMTDFSTLGDTFGQCFHADFDGISIVNFIVVISSFLFVDIFDTLGTLIGVCNKANMLDKDGKLPKIKPALLADSIATTAGAVLGTSTTTTFVESSAGVAAGGRTGFSAVVTAILFLISMFFAPIFIAIPSFATAPALVIVGFLMFSSITEISFKQTNYLHAIPAYLCVLAMPLFYSISEGISIGIISFVLLHLVCGKGKEVKPLLYVLAMLFLLKYIFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJK6</accession>
    <name>R5VJK6_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal RNA small subunit methyltransferase A</fullName>
            <ecNumber evidence="6">2.1.1.182</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">16S rRNA dimethyladenosine transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">16S rRNA dimethylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rsmA</name>
        <name evidence="6" type="synonym">ksgA</name>
        <name evidence="9" type="ORF">BN566_01457</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>adenosine(1518)/adenosine(1519) in 16S rRNA + 4 S-adenosyl-L-methionine = 4 H(+) + N(6)-dimethyladenosine(1518)/N(6)-dimethyladenosine(1519) in 16S rRNA + 4 S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:19609"/>
            <dbReference type="Rhea" id="RHEA-COMP:10232"/>
            <dbReference type="Rhea" id="RHEA-COMP:10233"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74493"/>
            <dbReference type="EC" id="2.1.1.182"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.182" evidence="6"/>
    <dbReference type="EMBL" id="CBAR010000156">
        <property type="protein sequence ID" value="CCZ83721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01457"/>
    <dbReference type="EnsemblBacteria" id="CCZ83721">
        <property type="protein sequence ID" value="CCZ83721"/>
        <property type="gene ID" value="BN566_01457"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052908">
        <property type="term" value="F:16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00607">
        <property type="entry name" value="16SrRNA_methyltr_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001737">
        <property type="entry name" value="KsgA/Erm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023165">
        <property type="entry name" value="rRNA_Ade_diMease-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020596">
        <property type="entry name" value="rRNA_Ade_Mease_Trfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020598">
        <property type="entry name" value="rRNA_Ade_methylase_Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011530">
        <property type="entry name" value="rRNA_adenine_dimethylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727">
        <property type="entry name" value="PTHR11727"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00398">
        <property type="entry name" value="RrnaAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00650">
        <property type="entry name" value="rADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00755">
        <property type="entry name" value="ksgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01131">
        <property type="entry name" value="RRNA_A_DIMETH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51689">
        <property type="entry name" value="SAM_RNA_A_N6_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 6 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 6" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="4 6 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="rADc" evidence="8">
        <location>
            <begin position="36"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ83721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="286" mass="31644" checksum="43EFC9774AD8790C" modified="2013-07-24" version="1">MEQSLTNMRYVRTLLERHGFHFSKKLGQNFLINPDVCPRIAEMGNAKPEHGILEIGTGIGTLTAELAKRAEKVTAVEVDARLFPILEETVGQYENLHIIHQDILKCDIPALLEQEFGTMPVSVCANLPYYITAPILMHLLESRAKLETITVMVQKEVAEKLTAPVGTREAGAITVAVQYYGTVTQLFSVSRGSFLPPPNVDSAVIQIQLGTSYAEQVADEKHFFRMVRSGFSQRRKTLVNALSATMGYEKAVVSKALTDCGLSETVRIESLSMEQLIALHRALLEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJQ8</accession>
    <name>R5VJQ8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01516</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000161">
        <property type="protein sequence ID" value="CCZ83781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83781">
        <property type="protein sequence ID" value="CCZ83781"/>
        <property type="gene ID" value="BN566_01516"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="68" mass="7858" checksum="3BB501EA64FC3440" modified="2013-07-24" version="1">MESYEKPYFQLFCTVCDVIENVELFLGNAALTGIQQQFLRKQVEQHPRIISHPLAYISIRNSTDERGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJR9</accession>
    <name>R5VJR9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pyruvate:ferredoxin oxidoreductase and related 2-oxoacid:ferredoxin oxidoreductases beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01526</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000162">
        <property type="protein sequence ID" value="CCZ83791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01526"/>
    <dbReference type="EnsemblBacteria" id="CCZ83791">
        <property type="protein sequence ID" value="CCZ83791"/>
        <property type="gene ID" value="BN566_01526"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TPP_enzyme_C" evidence="1">
        <location>
            <begin position="50"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="252" mass="27151" checksum="949CD5D3B6513080" modified="2013-07-24" version="1">MAVVFERPKALLDVPMHFCPGCGHGIVHRLVCEVLDEMGIEGNTIGVVPVGCSVMSYDYFGCDVIEAPHGRAPAVATGVKRSNPDKMVFTYQGDGDLAAIGTAETVHSGTRGENLVIIFINNTTYGMTGGQMAPTTMPGQVTQTTPYGRDPKYAGYPIRVCEMMSTLTGVALAQRVAVDSVPHIREAKKAIRKAFENEKAEKGLSIIEVLSTCPTNWGMAPQEAMQFVKDKMSHYYPLGVYKDGAAGEDEQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5VK46</accession>
    <name>R5VK46_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">zf-trcl domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01640</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000172">
        <property type="protein sequence ID" value="CCZ83911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01640"/>
    <dbReference type="EnsemblBacteria" id="CCZ83911">
        <property type="protein sequence ID" value="CCZ83911"/>
        <property type="gene ID" value="BN566_01640"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026363">
        <property type="entry name" value="CxxC-x17-CxxC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025306">
        <property type="entry name" value="Zn-bnd_dom_prob"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13451">
        <property type="entry name" value="zf-trcl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04272">
        <property type="entry name" value="cxxc_cxxc_Mbark"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="zf-trcl" evidence="1">
        <location>
            <begin position="3"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="94" mass="10642" checksum="E823F8F7DFE4EEC5" modified="2013-07-24" version="1">MYEDKTLVCKDCGAEFVFTAGEQEFYAEKGFVNEPKRCKSCRDAKKNASGNKPEREMFTATCAACGKEARVPFQPREDRAVYCSECFAKMKEAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5VKK8</accession>
    <name>R5VKK8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01747</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000180">
        <property type="protein sequence ID" value="CCZ84021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84021">
        <property type="protein sequence ID" value="CCZ84021"/>
        <property type="gene ID" value="BN566_01747"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="120" mass="13778" checksum="8EB8F46D33FEB796" modified="2013-07-24" version="1">MIEAWIAQQLIKGLLRSLLLTEVLELLAAACFPQRNRHDFLLVILVNILTNPVVVYLDFWLHGATIAWRWLWVGGLEFAVWLSEALIYRKCLIGKQNPYLFSLILNAASYLGGVCLQYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5VKU9</accession>
    <name>R5VKU9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thiazole synthase</fullName>
            <ecNumber evidence="4">2.8.1.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_01876</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + [sulfur-carrier protein ThiS]-C-terminal Gly-NH-CH2-C(O)SH = 2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS]-C-terminal Gly-Gly + 2 H(+) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:26297"/>
            <dbReference type="Rhea" id="RHEA-COMP:12908"/>
            <dbReference type="Rhea" id="RHEA-COMP:12909"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57792"/>
            <dbReference type="ChEBI" id="CHEBI:62899"/>
            <dbReference type="ChEBI" id="CHEBI:77846"/>
            <dbReference type="ChEBI" id="CHEBI:90619"/>
            <dbReference type="ChEBI" id="CHEBI:90778"/>
            <dbReference type="EC" id="2.8.1.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; thiamine diphosphate biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.10" evidence="4"/>
    <dbReference type="EMBL" id="CBAR010000193">
        <property type="protein sequence ID" value="CCZ84151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01876"/>
    <dbReference type="EnsemblBacteria" id="CCZ84151">
        <property type="protein sequence ID" value="CCZ84151"/>
        <property type="gene ID" value="BN566_01876"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00060"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990107">
        <property type="term" value="F:thiazole synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033983">
        <property type="entry name" value="Thiazole_synthase_ThiG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008867">
        <property type="entry name" value="ThiG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34266">
        <property type="entry name" value="PTHR34266"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05690">
        <property type="entry name" value="ThiG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0784">Thiamine biosynthesis</keyword>
    <feature type="domain" description="ThiG" evidence="6">
        <location>
            <begin position="2"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ84151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="82" mass="8215" checksum="F1A40352649FB602" modified="2013-07-24" version="1">MKLPIIVDAGIGAPSQACEAMEMGAAAVMANTAVATAGDCAAMATAFRLAIEAGRLAYLSKLGRVLDKGAEASSPLTGFLQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>R5VKY2</accession>
    <name>R5VKY2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transcriptional regulator AraC family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01916</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01916"/>
    <dbReference type="EnsemblBacteria" id="CCZ84191">
        <property type="protein sequence ID" value="CCZ84191"/>
        <property type="gene ID" value="BN566_01916"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003313">
        <property type="entry name" value="AraC-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037923">
        <property type="entry name" value="HTH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02311">
        <property type="entry name" value="AraC_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51215">
        <property type="entry name" value="SSF51215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="3">
        <location>
            <begin position="190"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="297" mass="34269" checksum="6DB51201FB715071" modified="2013-07-24" version="1">MKKQQLLENKQHGTPAFPFECFRTQTDTTDFFIPLHWHKKTELMHVHHGTCLLTIGNETYPAKGGDLFCINAEELHRITSEDATLEYSTFIFQPDLFSFAISDQAQLLLQPLIDGRMQFPTCITDAAVKATLQPLLHRILQAAEEKAVGYELLVKAGLLQLTAELLYQGLLLPTDPITLEASRNQRERMKKILTYLKQHMTEPLTLSSLADTFHMSEKYFSRYFRNATGQTFTAYLNTIRMNHACQLLLETDDTVLEIALECGYENVSYFIRLFGNLMHCTPLQYRLSKQHRLKQQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5VKZ2</accession>
    <name>R5VKZ2_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glft2_N domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01926</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01926"/>
    <dbReference type="EnsemblBacteria" id="CCZ84201">
        <property type="protein sequence ID" value="CCZ84201"/>
        <property type="gene ID" value="BN566_01926"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040492">
        <property type="entry name" value="GlfT2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17994">
        <property type="entry name" value="Glft2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glft2_N" evidence="1">
        <location>
            <begin position="33"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="613" mass="71534" checksum="875B42DBC1015E0B" modified="2013-07-24" version="1">MKLQNILFPSTDTCTEELMYFRRGDKVFYNWSSDGVEIQKDGILFFDTYFNGFTIEKWAKYTNVKTVFLTIRIEGHVRLTLMRKEKKGEKILTDFLGEYICKANYGEIKEFTFPFHSVSTNGIYCFNITGISEQSGFYGGFYSGDIAEKDIQPVKIAIDICTYKRERFIEKNLKLLNERFLENPDSFLQGKLEVFVSDNAHTLDIPALSSENIHIVQNKNVGGAGGFTRGMIEINRVKQQRHITHILVMDDDVTIEPESLFRTCTFLSCRKAHYQDLFIGGAMLRLDTQCIQTEAGAVWNGGELISLKHGLDLRELDACLYNELEEKTQFNAWWYCVFPSEVVTDQNLPMPIFIRGDDVEYGLRNMKNLVLMNGICVWHEPFENKYSSFLYYYILRNRLIDNALHDMSLSKKAFRHLLQQQVYNEMRLYRYKNANLLMQGVEDFLRGVNWLMEQDGEALHKEIMQLGYKLQYTEDLKEDVQFSFPIYEAACKAVPATKFHQRVINKLTMNGLRLPVKRQFNIVPTDGVQQSAVYRTQTILNYDYTSRKGFVTERDPEAAKQCFRRLKALYHQLDKLYDAAVRDYAENGKKLMQLSFWKQYLELPEKEPATMEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5VL28</accession>
    <name>R5VL28_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00015</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000197">
        <property type="protein sequence ID" value="CCZ84246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00015"/>
    <dbReference type="EnsemblBacteria" id="CCZ84246">
        <property type="protein sequence ID" value="CCZ84246"/>
        <property type="gene ID" value="BN566_00015"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025641">
        <property type="entry name" value="DUF4340"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14238">
        <property type="entry name" value="DUF4340"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004407160" evidence="1">
        <location>
            <begin position="32"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4340" evidence="2">
        <location>
            <begin position="112"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4340" evidence="2">
        <location>
            <begin position="274"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="523" mass="56248" checksum="D22B88614BD03BB5" modified="2013-07-24" version="1">MQKRMIGVICGAAVVVLLAGSVVLLTRPSGKEDSSSDATAATTQSAEDLAALTLSDESADNVKQISVTNATGSYEVVRVKTAKTTTATDATDATDSTAATTGTESADFRISGWEDIPTNESLLGTLANNTASLQADQCVEEHATDLDKFGLGKDATQVSMQFDDGTTFAFRIGGSVSGASCNYFALADSDTVYAVKTSLLANFSKADTDFLATTVLEKPDDDNMPTINTVTIERKDLDTPIVLEHRDTSNDGESGNTQSTHKMTSPISAYLSVERSKDVIEGLFGLSASKILAVRPSEQDLEDYGISDPFATLTLECEGGTTYTLKIGDVVTEADDISTDDSTATTSTATTYYPVQLDDGNVIYAIAADKCPWATITPTGLASKLIFTTYVWDISELDVTVDGKKTTFENSGTDKDTASITRDGKSEIDTERYRQFYAFLLQTAAEEVVLGDAPTGTPDAEIHYKTSDGSEDRTVAFYQTDDFNCYITVNGELSFQCRSSYLDALRHNLDIFDTDEDFQTSWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>R5VQA3</accession>
    <name>R5VQA3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator AraC family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00567</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000004">
        <property type="protein sequence ID" value="CCZ82762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00567"/>
    <dbReference type="EnsemblBacteria" id="CCZ82762">
        <property type="protein sequence ID" value="CCZ82762"/>
        <property type="gene ID" value="BN566_00567"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="1">
        <location>
            <begin position="145"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="260" mass="30551" checksum="0D1084B8DBF12AF2" modified="2013-07-24" version="1">MTYHQELQHRLFQQQEEQVHHMEYQQEFQYYEDVANGRLDRIHLQLLQPNDIVVYNDGEYGMLSKDPLRNLRYHFVVSVAMITRLCVEHGLEQELAYTMSDLYINKMDELQQAEAIISLQNDMLLDFTQKMADLPKRNGYSIHVIKGIEYICRHLHQRLTVAEVSAALSVNRSYFSALFAKETGCSVSQYIRQEKLQAAANMLRFSDYSYADIAEYFGFSSQSHFIQCFQKCYACTPAAFRKRYFQKTFTVPPTPEMPEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>R5VQV9</accession>
    <name>R5VQV9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Sigma-70 region 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_00742</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000029">
        <property type="protein sequence ID" value="CCZ82942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82942">
        <property type="protein sequence ID" value="CCZ82942"/>
        <property type="gene ID" value="BN566_00742"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="3">
        <location>
            <begin position="22"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="4">
        <location>
            <begin position="117"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ82942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="185" mass="21322" checksum="C5B8958B445B624B" modified="2013-07-24" version="1">MRESRLITLLQLQKPEGLQVLISTYQAYVGTIIRNITRSSLSEQDVEELTADTFFAVWQTTDKLQAGKVRAYLAAIARNKAKSRLRSLSETIPLEEEAILLETADLEQEVEHTLLSEALQDTITTLSATDRDVLIRYYYYYQRIPEIAEELQLSVSAVKVRLHRARKKLKQLLIERGYVYETESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5VR42</accession>
    <name>R5VR42_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Branched-chain amino acid transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN566_00790</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AzlC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000036">
        <property type="protein sequence ID" value="CCZ82997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82997">
        <property type="protein sequence ID" value="CCZ82997"/>
        <property type="gene ID" value="BN566_00790"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011606">
        <property type="entry name" value="Brnchd-chn_aa_trnsp_permease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34979">
        <property type="entry name" value="PTHR34979"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03591">
        <property type="entry name" value="AzlC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="59"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="160"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="187"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="212"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ82997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="239" mass="25264" checksum="78ABA61A3C6F2F7D" modified="2013-07-24" version="1">MTVQHKQAFWKGVRQGIPIALGYLSVSFSIGILAVKAGLSVSAAVAISMTNVTSAGQAAGIGVIAAGGSYLELALTQFCINLRYALMGLSLSQKLDQTFQHMIHRFLTAFGITDEIFAVAVSQDGQISAPYLYGLMSLPLIGWSLGTFLGAAAGEILPKIITDALGIALYGMFFAIFIPPMRKQKSIVFAVLVAAGCSIICKYCLPFISSGFAIILCGVLTAALCAWLFPRKEEQGVAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5VR53</accession>
    <name>R5VR53_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">D-alanyl-D-alanine carboxypeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00805</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000038">
        <property type="protein sequence ID" value="CCZ83012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00805"/>
    <dbReference type="EnsemblBacteria" id="CCZ83012">
        <property type="protein sequence ID" value="CCZ83012"/>
        <property type="gene ID" value="BN566_00805"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1380.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009045">
        <property type="entry name" value="Hedgehog_sig/DD-Pept_Zn-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003709">
        <property type="entry name" value="Pept_M15B"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02557">
        <property type="entry name" value="VanY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55166">
        <property type="entry name" value="SSF55166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="VanY" evidence="3">
        <location>
            <begin position="134"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="361" mass="39514" checksum="D11E2A7EFA1E4974" modified="2013-07-24" version="1">MSAWDTTQHANHPNPQPSGNRPPRRKKPRIRYDRIVLVALVLIGLICLLVLGVKQCGKKDTTGNSSSGAAATTTQISTEPLERTLALTAEDVHKGSLILVNADHVYTFAADDVSLETVYEGRNSCYGVSDMEVSLDADVIDHFNQLMQAYYNIYDNNDIMIVSGYRTREEQAAYYESKKTMLPGGYSDYHTARSFDLVVYPNGTTSNYYAATGDYAWIAEHAAEYGFVLRYPDGKADKTGISPRAYTFRYVGVPHALYMYENGLCLEEYLEEIQGYSADKPLTIDTDGGEWNVFYMQANTSGSTQIAVPQDATYTVSGDNVGGFIVAYQEAGTAAVTTAVDATGDAAETTVVPETTAENAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5VRN8</accession>
    <name>R5VRN8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl hydrolase family 25</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01005</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 25 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000076">
        <property type="protein sequence ID" value="CCZ83222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01005"/>
    <dbReference type="EnsemblBacteria" id="CCZ83222">
        <property type="protein sequence ID" value="CCZ83222"/>
        <property type="gene ID" value="BN566_01005"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003796">
        <property type="term" value="F:lysozyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016998">
        <property type="term" value="P:cell wall macromolecule catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009253">
        <property type="term" value="P:peptidoglycan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1380.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002053">
        <property type="entry name" value="Glyco_hydro_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009045">
        <property type="entry name" value="Hedgehog_sig/DD-Pept_Zn-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013230">
        <property type="entry name" value="Peptidase_M15A_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01183">
        <property type="entry name" value="Glyco_hydro_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08291">
        <property type="entry name" value="Peptidase_M15_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55166">
        <property type="entry name" value="SSF55166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51904">
        <property type="entry name" value="GLYCOSYL_HYDROL_F25_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_M15_3" evidence="2">
        <location>
            <begin position="3"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="371" mass="41144" checksum="9D2B203AFDF8D641" modified="2013-07-24" version="1">MVDKLEALYTALNCSKIIVTSGYRCPDHDKAVGGTSSGQHTKGTAADVCCYGQDGQPISSKTVCCKAQDLGFGGIANITAAYQYTHLDVRTGYRWLGDEVKGNGTVTEDFYKYFGIKKTTETTSILKGIDVSYCQNEVDWDAAKASGLVDFAILRAGYGRETTQVDTQFERNYAACKRLGIPCGAYWFSYAMSADEARREAQVFLQTIKGKSFEYPVYMDLELAKQFALGKAACSAIVDAFLSVLEQSGYYAGLYCSTYYLDNYLSDSIKSRYTVWCAQYASKCTYQNPYGIWQYNVAGNEEYDIIGQKSIPGIVGECDMDYAYKDYPTIIKTAGLNGFTKVTQPNEPEPDTEESTLQQILKHVANIDEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5VRV9</accession>
    <name>R5VRV9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01078</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83297">
        <property type="protein sequence ID" value="CCZ83297"/>
        <property type="gene ID" value="BN566_01078"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="355"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="598" mass="67287" checksum="5CEADB65379B5906" modified="2013-07-24" version="1">MQQVCKCCGRTMEVSETAYTATCHFCGKQQTLPPCHTPKLRKQYAQIYALRQSGAFSAAIEQIEHLLHLYPEESEWYWQRLLCRYGMVYLPDIGKKTYSLQCTQPLDCPISEDADYLAALQFAKPETEHYYEADATLLEQERLEECHPSDPEKNAEWLLESGFQSLEAEHWQLATAQFDLLLQRQPENANAHLGKMMAQLQVRREEELLKTGNAMEQTEQYAELMQHADPAFREKILQYEKKAKYQQAVLMKEKANSVEALQAAVDLLETIPNDPDAERLIVVCKRQMRERMLAYSEVLIGCHAAEHLILSGESEEKRIIVAKYYYDSDTEEEEQAAAAAERHAAPLKIIDLRTILLLIAVVGLAAALVIVLFFQKEAPQVQPERHTYPAIPSTSTLLETNSSHSATTETKQSVKADAKIESNPIVSQIEPTQALFTADADAIACGEYRYVLSEGNLYRIEAVGSNSGQEKILEQIQEILPDQDERLLYLQAGTVGFLQANGDFGDAISNDNIKKLFLLPTNDPQTSVFLLSEDGMLSAFQYIAETNRYEPQAIFMDADAVTAQVSGWETVANLEIIDHILIGTDEKGAPVHAIPLQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5VSA0</accession>
    <name>R5VSA0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01188</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000098">
        <property type="protein sequence ID" value="CCZ83412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83412">
        <property type="protein sequence ID" value="CCZ83412"/>
        <property type="gene ID" value="BN566_01188"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="168" mass="19272" checksum="A12947CEE283704F" modified="2013-07-24" version="1">MTKPTTTTTTTTEATTTTTKPTTTTTTTTETTTTMTKPTTTTTTTTEATTTTTKPTTTTTTTTETTTTMTKPTTTTTTTTEATTTTTNHHHNDNHRSNNYHNNKTDYNDNHNHNNQTDYNHHNDNNDNDNHYYNHNDYGNNHHRRNNYNHRGSNHHRRSHYDRRSDYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>R5VSB4</accession>
    <name>R5VSB4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Carbohydrate ABC transporter membrane protein 1 CUT1 family (TC 3.A.1.1.-)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_01196</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000102">
        <property type="protein sequence ID" value="CCZ83422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01196"/>
    <dbReference type="EnsemblBacteria" id="CCZ83422">
        <property type="protein sequence ID" value="CCZ83422"/>
        <property type="gene ID" value="BN566_01196"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="24"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="180"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="219"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="274"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="83"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ83422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="316" mass="35460" checksum="8D839FE9997DA0D3" modified="2013-07-24" version="1">MAGSGKTGSTSLRNKLHKMQTQKVIVSILFLCVPLLLLFTFTYLPFFKMVQFSFYDMKYIGKRTWVGLQNYISVFTREDCLNALKLSLFYLVGSFVQIAIALLFASILSFPTKGGKFFRGALYFPSLIGGIAVGFIFKFFFTHGFVLDTLLTWVGMDADSLPFWLKDKSINNWVLAATSIWRYMGQNMVLFIGAIASVDTTQYEAAAIDGANAWQRFRYIILPSIKTIVTLNLILAVSGALSAFEMPYVITDGGFGTSTYFVLMNKIAHTNQKVGLASAMAVVLLALILLVTAVQKIVERRMDSDTPRRRKRRVQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5VSF2</accession>
    <name>R5VSF2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01231</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000109">
        <property type="protein sequence ID" value="CCZ83457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01231"/>
    <dbReference type="EnsemblBacteria" id="CCZ83457">
        <property type="protein sequence ID" value="CCZ83457"/>
        <property type="gene ID" value="BN566_01231"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="384" mass="43071" checksum="EE11BC6A45C0EF16" modified="2013-07-24" version="1">MFPLSLFHRKKPAAAPSVLISAERQQHRDFSLLRQDETAQQLYRQLRYAVPIIDAALSKIVRLTGSYSVIASDPAVQKQLDAFVQQIPCDFSSQGLQSFTDRFLDSLLTCGNALGELLVDSRRRCITGLHCAEPDLVCLKPGKSDRAFFLRMTDGSPEERPLPHPERLLFCALHPPAGQIYGVSILHGIPALCSILLRIYECIGQNYDRIGNIRYAVTYHPSDDPTERAYTTERVKAIAKEWAAGMRDSSSGEVRDFICAGDVDIKVIGAENPLLDTEIPVRQLLEQIVSKLSIPPFLLGLNWSSTERMSTQQADILTSELEYYRRLLTPVIQQICTAFLRTIGSTAEISVEWDTINLQDETEQALARLHTAQAMQIEQNLQKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5VSL7</accession>
    <name>R5VSL7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_01271</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000119">
        <property type="protein sequence ID" value="CCZ83502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01271"/>
    <dbReference type="EnsemblBacteria" id="CCZ83502">
        <property type="protein sequence ID" value="CCZ83502"/>
        <property type="gene ID" value="BN566_01271"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="4"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="42"/>
            <end position="49"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="254" mass="27751" checksum="186410FD20FDF91E" modified="2013-07-24" version="1">MAMLQVECLKKIYTTRFGGNQVQALSDVSFSVETGEYVAIMGESGSGKTTLLNILAALDQPTSGKVFLDGKDLTMVKEREIAAFRRDNLGFVFQDFNLLDTFSLKDNIFLPLVLAGKSPKEMELRLNPIAEQLGITHLLEKYPYEVSGGQKQRAAVARALISKPKLILADEPTGALDSKSTDGLLDVFSAIHATGQTILMVTHSTKAASRADRILFIKDGVLFHQIYRGGCTNEQLYQKISDTLTMLATGGEQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>R5VSQ9</accession>
    <name>R5VSQ9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA mismatch repair protein MutS</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">mutS</name>
        <name evidence="10" type="ORF">BN566_01330</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">This protein is involved in the repair of mismatches in DNA. It is possible that it carries out the mismatch recognition step. This protein has a weak ATPase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the DNA mismatch repair MutS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000131">
        <property type="protein sequence ID" value="CCZ83562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01330"/>
    <dbReference type="EnsemblBacteria" id="CCZ83562">
        <property type="protein sequence ID" value="CCZ83562"/>
        <property type="gene ID" value="BN566_01330"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00096">
        <property type="entry name" value="MutS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005748">
        <property type="entry name" value="DNA_mismatch_repair_MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007695">
        <property type="entry name" value="DNA_mismatch_repair_MutS-lik_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017261">
        <property type="entry name" value="DNA_mismatch_repair_MutS/MSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007861">
        <property type="entry name" value="DNA_mismatch_repair_MutS_clamp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016151">
        <property type="entry name" value="DNA_mismatch_repair_MutS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007860">
        <property type="entry name" value="DNA_mmatch_repair_MutS_con_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036678">
        <property type="entry name" value="MutS_con_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS_family"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="PTHR11361"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01624">
        <property type="entry name" value="MutS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05188">
        <property type="entry name" value="MutS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05192">
        <property type="entry name" value="MutS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05190">
        <property type="entry name" value="MutS_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037677">
        <property type="entry name" value="DNA_mis_repair_Msh6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="SSF48334"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53150">
        <property type="entry name" value="SSF53150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55271">
        <property type="entry name" value="SSF55271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01070">
        <property type="entry name" value="mutS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7 8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6 7 8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA_MISMATCH_REPAIR_2" evidence="9">
        <location>
            <begin position="703"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="629"/>
            <end position="636"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ83562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="872" mass="98569" checksum="DF46D3C27C46D848" modified="2013-07-24" version="1">MEIQKEKLSPMMQQYFALKEKYPDCVLFFRLGDFYEMFFDDAIRISRELELTLTGRDCGLKERAPMCGIPYHSVDMYLKRLVERGYKIAICEQLTDPATTKGLVERDVIRIVTPGTLIESNLLEEGANNYLCSLYFEEGGDCGLCFADLSTGDIIALLPEQKNLEANVIDALSRYTPAEILMNEDALLLKSVMEFIKIRLQCVVTMRDAECFSAERNHDFVCHQFQVPELSYLQLPETGSETAAVYGMMDYIKETQKNQIARFISLTVNDDDAYLGLDFNARRNLELTETLRGKERKGSLLWLMNTAKTSMGKRMLKTWIEQPLVQPAKIMARLDAVEIFVKKSVVLMEVRSILDSVYDLERLMTRVLYKTATPRDLKALATTARQIPALKAELEKVADRKLLARLLSQISNLDMVADLVDRAIVENPPVSVKDGGVIREGFHQDLDYLRGIMNGGNQIIEKMEQSERERTGIKGLKIGFNRVFGYYIEVTRSYYDLVPDDYVRKQTLTNCERFITPDLKKTENDILSAKEKALRLEESLYVEVRDCLAAQLKQVQETASAIAQVDVLAAFANVAIENQYTKPEIAMDGVIQIRGGRHPVVERMLSDDIFVPNDTYLDNKANRMLLITGPNMAGKSTYMRQVALITLMAQIGSFVPADYAKISVVDKIFTRVGASDDLTAGQSTFMVEMHEVSDILKYATPNSLVILDEVGRGTSTFDGVSIARAVAEYICNSRKIGCKTLFATHYHELIDLEHTIEGVKNYSIAVKKHGDTIRFLRKIVPGGIDDSYGIEVAKLAGLPEKVVKRARVLLRQMEQQTAAQNQKPEQSDDMQYNFAAMQQEQAVQMLKKTNLQELSDAECRQVLEEVTNLLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>R5VSU2</accession>
    <name>R5VSU2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Binding-protein-dependent transport systems inner membrane component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_01365</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000144">
        <property type="protein sequence ID" value="CCZ83602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01365"/>
    <dbReference type="EnsemblBacteria" id="CCZ83602">
        <property type="protein sequence ID" value="CCZ83602"/>
        <property type="gene ID" value="BN566_01365"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="23"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="84"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="118"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="274"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="80"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ83602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="302" mass="33706" checksum="ED7770A4BA5D8306" modified="2013-07-24" version="1">MSEIKKQKKKETFAPIGKVSNRWGWGFVSISVIMISVLVFIPMIQSFLLSLQSGQGNQLTFAGLSNYKRMLTDTTFRKALVNTFLYLLVQVPVMIILALFISVLLNDKKLKCRSFFRIAIFLPCVTSLVSYSLIMKSIFSGDGLVNQILMAMHLISEPIGWTTSPAFAKALIIISITWRWTGYNMIFYLSGLQNIDPSIYEAAELDGCNGFQKFFKIIVPQLKPIILFTTITSTIGTLQLFDEVKNITDGGPANTTLTISQYIYNLCFKYTPNFGYAAAIAFVVLVMIAILSAIQMKVGGDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>R5VSU7</accession>
    <name>R5VSU7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Polyamine aminopropyltransferase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Putrescine aminopropyltransferase</fullName>
            <shortName evidence="3">PAPT</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Spermidine synthase</fullName>
            <shortName evidence="3">SPDS</shortName>
            <shortName evidence="3">SPDSY</shortName>
            <ecNumber evidence="3">2.5.1.16</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">speE</name>
        <name evidence="6" type="ORF">BN566_01370</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the irreversible transfer of a propylamine group from the amino donor S-adenosylmethioninamine (decarboxy-AdoMet) to putrescine (1,4-diaminobutane) to yield spermidine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>putrescine + S-adenosyl 3-(methylsulfanyl)propylamine = H(+) + S-methyl-5'-thioadenosine + spermidine</text>
            <dbReference type="Rhea" id="RHEA:12721"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:57443"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
            <dbReference type="ChEBI" id="CHEBI:326268"/>
            <dbReference type="EC" id="2.5.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amine and polyamine biosynthesis; spermidine biosynthesis; spermidine from putrescine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer or homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the spermidine/spermine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.16" evidence="3"/>
    <dbReference type="EMBL" id="CBAR010000144">
        <property type="protein sequence ID" value="CCZ83607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01370"/>
    <dbReference type="EnsemblBacteria" id="CCZ83607">
        <property type="protein sequence ID" value="CCZ83607"/>
        <property type="gene ID" value="BN566_01370"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00248">
        <property type="reaction ID" value="UER00314"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004766">
        <property type="term" value="F:spermidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008295">
        <property type="term" value="P:spermidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.140.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00198">
        <property type="entry name" value="Spermidine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030374">
        <property type="entry name" value="PABS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001045">
        <property type="entry name" value="Spermi_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035246">
        <property type="entry name" value="Spermidine_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037163">
        <property type="entry name" value="Spermidine_synt_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11558">
        <property type="entry name" value="PTHR11558"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17284">
        <property type="entry name" value="Spermine_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00417">
        <property type="entry name" value="speE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51006">
        <property type="entry name" value="PABS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0745">Spermidine biosynthesis</keyword>
    <keyword evidence="1 3 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PABS" evidence="5">
        <location>
            <begin position="3"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-methyl-5'-thioadenosine binding" evidence="3">
        <location>
            <begin position="138"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Spermidine binding" evidence="3">
        <location>
            <begin position="156"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3 4">
        <location>
            <position position="156"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>S-methyl-5'-thioadenosine</name>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>spermidine</name>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>spermidine</name>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>S-methyl-5'-thioadenosine</name>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ83607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="285" mass="32956" checksum="020B8BCB0821B438" modified="2013-07-24" version="1">MMDIWYTENHTDHVRMSMRVDKQLASVQSDFQRIDIFESVEFGRIMTLDGLLMLTEKDEYIYHEMITHIPMATNPNIRNILVIGAGDGGTVRELVRYPSIEHIDVAEIDEMVVELSKKYLPFTACGFNDPRVDVHIQDGLRFVRKVENEYDLIIVDSTDPFGVGEGLFTKEFYGNCYKALKADGILVNQHESTFYTSYANSMKRAHSRIKSFFPIALVYQAHIPTYPSGHWLFGFASKKYDPRTDLQADWWNSLGIRTRYYNTILHQGCFAIPNNVRDALRESET</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5VTD8</accession>
    <name>R5VTD8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01537</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000164">
        <property type="protein sequence ID" value="CCZ83802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01537"/>
    <dbReference type="EnsemblBacteria" id="CCZ83802">
        <property type="protein sequence ID" value="CCZ83802"/>
        <property type="gene ID" value="BN566_01537"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004509">
        <property type="entry name" value="Competence_ComEA_HhH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003583">
        <property type="entry name" value="Hlx-hairpin-Hlx_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="SMART" id="SM00278">
        <property type="entry name" value="HhH1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="SSF47781"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00426">
        <property type="entry name" value="TIGR00426"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HhH1" evidence="2">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="HhH1" evidence="2">
        <location>
            <begin position="145"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="HhH1" evidence="2">
        <location>
            <begin position="213"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="177"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="268" mass="29348" checksum="068B96950CAE71D8" modified="2013-07-24" version="1">MMGTAVVVLAVQSFAEQDTIPAITWVTTASEESAESALQTEPLETEADSENANRQPVFDWDKPTKAETTTKSAGSTTAPVRQTEPAQPDAAELAFPEEAEPEISYPLDLNAATAEELETLPGVGAILAERIVSYREAVGGFQTLEELQQVNGIGSGIYSQIAPYLFIIGELQTISPELDTQPSESSEPTMPEEKTASDSESIPHLDINTATAEDFQKLPNVTQEQAEAIVRLRTQIQYFQNIYELLYADGMTDRLFLSIRDYLYVSFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>R5VTW8</accession>
    <name>R5VTW8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phosphoribosyl pyrophosphate synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_01691</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the ribose-phosphate pyrophosphokinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000176">
        <property type="protein sequence ID" value="CCZ83962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01691"/>
    <dbReference type="EnsemblBacteria" id="CCZ83962">
        <property type="protein sequence ID" value="CCZ83962"/>
        <property type="gene ID" value="BN566_01691"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009165">
        <property type="term" value="P:nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029099">
        <property type="entry name" value="Pribosyltran_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
        <property type="entry name" value="PTHR10210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13793">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pribosyltran_N" evidence="5">
        <location>
            <begin position="23"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Pribosyltran" evidence="4">
        <location>
            <begin position="210"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00029942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ83962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="388" mass="43832" checksum="BE77520C7BCE1850" modified="2013-07-24" version="1">MVTASSSASDILFNHETNVAPLGLIAMRGTEELGKKIDSYLVEWARKGGYDVDTFLIACECPRFSSGDGKGLIKSTIRGRDLFILIDVGNYSCEYQMFDRMNVMSPDDHYQDLKRIIQAASGKAHRVNVIMPILYGGRQHRRNYRESLDCACALQELEQMGVSNIITFDAHDPRVCNAIPLMGFDNVVPSYQVLKAMFHDIPHLRTTPDEFMVISPDEGALNRNMYYASMLGVPMGMFYKRRDYSIIVNGRNPIVAHEYLGNSVEGKDVFIADDIISSGESMLDIAYALKKRKARRVFCYATYGLFVNGLQKFDDAYANGYIDAVFGTNLTYRTKELLSREWFHEVDCSKYISFFIAALNHDASIGNVIDSNQKIKALLARRNQEEAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5VU36</accession>
    <name>R5VU36_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycos_transf_1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00172</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000179">
        <property type="protein sequence ID" value="CCZ84012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00172"/>
    <dbReference type="EnsemblBacteria" id="CCZ84012">
        <property type="protein sequence ID" value="CCZ84012"/>
        <property type="gene ID" value="BN566_00172"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="174"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="356" mass="40214" checksum="4F2D5059F84F0A3C" modified="2013-07-24" version="1">MRVIQLLPTISMGDAVSNDALAIAKVLRDMGYQTGIYAENIDNRLPAGTAKPVSKMPRLQTEDAVLYHMSTGSALNEMIPKLRCKKGMIYHNITPSYFFQPYNAKLTQLLKDGLTGVLNLVGAFDFCLADSEFNKQALLEMGYTCPIAVRPVLIPFSDYAKKPTQEIIDRYDNDGYVNFIFVGRIAPNKKQEDVIRAFSCYQRFCNPKSRLILVGSWSGAESYYRRLCRYTAALQAENVLFTGHIPFPDILAYYHVADLFLCMSEHEGFCVPLVEAMYFGVPILAYRACAVPDTLGDSGILLDKKDPMETAMVADRILTDTSLRRELVARQRRRQQDFSYGAVRALLESQLREFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>R5VUC2</accession>
    <name>R5VUC2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">cAMP-binding proteins-catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01838</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000187">
        <property type="protein sequence ID" value="CCZ84112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01838"/>
    <dbReference type="EnsemblBacteria" id="CCZ84112">
        <property type="protein sequence ID" value="CCZ84112"/>
        <property type="gene ID" value="BN566_01838"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012318">
        <property type="entry name" value="HTH_CRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13545">
        <property type="entry name" value="HTH_Crp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="SSF51206"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="13"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="220" mass="24805" checksum="2BE455E58F00CF58" modified="2013-07-24" version="1">MQQYLEILQTCRLFAEIPPDSLKTMLECFAPTIQTYAKQQTLLQAGEPLHQLGIVLTGKVQLVRLDYDGNRSITASLSPAQPFGEAFVCAGVDAAPISVIAATESTILWLEGEKLLHPCCNACGFHQQLLFNVIQLLARKNVLFHQKLEITAKRTTREKLLAYLFQQAKQENSNTFVIPYDRQGLADYLEVDRSGLSAEISKLRKEGILESKKNWFRLHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>R5VUE1</accession>
    <name>R5VUE1_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BN566_01857</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3"/>
    <dbReference type="EMBL" id="CBAR010000191">
        <property type="protein sequence ID" value="CCZ84132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01857"/>
    <dbReference type="EnsemblBacteria" id="CCZ84132">
        <property type="protein sequence ID" value="CCZ84132"/>
        <property type="gene ID" value="BN566_01857"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004589">
        <property type="entry name" value="DNA_helicase_ATP-dep_RecQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006293">
        <property type="entry name" value="DNA_helicase_ATP-dep_RecQ_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010997">
        <property type="entry name" value="HRDC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002121">
        <property type="entry name" value="HRDC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044876">
        <property type="entry name" value="HRDC_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032284">
        <property type="entry name" value="RecQ_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018982">
        <property type="entry name" value="RQC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00570">
        <property type="entry name" value="HRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16124">
        <property type="entry name" value="RecQ_Zn_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09382">
        <property type="entry name" value="RQC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00341">
        <property type="entry name" value="HRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00956">
        <property type="entry name" value="RQC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47819">
        <property type="entry name" value="SSF47819"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01389">
        <property type="entry name" value="recQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00614">
        <property type="entry name" value="recQ_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50967">
        <property type="entry name" value="HRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="24"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="214"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="HRDC" evidence="10">
        <location>
            <begin position="536"/>
            <end position="616"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CCZ84132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="617" mass="69848" checksum="44878613A5749E9E" modified="2013-07-24" version="1">MDKHEALQQYFGHATFRDGQEEVVDAILQGRDVLGIMPTGAGKSVCYQLSAMLLSGLTIVISPLISLMKDQVDSLLEIGISAAYLNSSMDAGEYQVTMLQARQGIYRILYVAPERLQTEGFLSFAEQAEISMVTVDEAHCVSQWGQDFRQSYLGIAEFLNKLQKRPIVSAFTATATEQVCKDICRLLQLQDPFQITTGFDRKNLYFAVRTPRNKERELLELLQERRGKSGIVYCLARKTVEELCDRLCEQGFPATRYHAGLSQAERAANQEAFLFDTKPIMIATNAFGMGIDKSNVSFVVHYNMPKDLESYYQEAGRAGRDGEPADCILLYSGRDVQTHLFLLDQAREISEIQDAQQKEQWLTRSKERLRIMAGYATTTNCLREYLLRYFGERAPQCCGHCSNCQGTFETTDITLEAKKIISCVYRGLQHHYHLSRTLAADVLTGSKKAAISEMRLNQLSTYGILKECTARQVVQMIDALLDLHILELQTYRDFQMLQLTPAAAEVIRGNQQVLWRRRKEERKTVFIPKPKPTVEEDVEEVLFQRLSALRAKLAEKEHMPAYIVFSNAALRDMCRKKPSSLAAFRQVSGVGIIKSQKYGKAFIKEIAAYTAEKSADK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVN3</accession>
    <name>R5VVN3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl hydrolase family 25</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00423</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 25 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000246">
        <property type="protein sequence ID" value="CCZ84562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00423"/>
    <dbReference type="EnsemblBacteria" id="CCZ84562">
        <property type="protein sequence ID" value="CCZ84562"/>
        <property type="gene ID" value="BN566_00423"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003796">
        <property type="term" value="F:lysozyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016998">
        <property type="term" value="P:cell wall macromolecule catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009253">
        <property type="term" value="P:peptidoglycan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002053">
        <property type="entry name" value="Glyco_hydro_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01183">
        <property type="entry name" value="Glyco_hydro_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51904">
        <property type="entry name" value="GLYCOSYL_HYDROL_F25_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="203"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="244" mass="27196" checksum="A8002E6732DD68B2" modified="2013-07-24" version="1">MGKICGMDVSYAQGSINWEQVKASKKIEFAILRAGYGRETSQVDTQFERNYAACKRLGIPVGVYWYSYATTAAEAEQEAKVCLQTIRGKQFEYPVAFDIEEARSLPQADALCTAFCNALEKQGYYAAIYTFKSALENNISAAIKSRYDIFLSHVGVQQTSYAGAYGLWQYSWTGRIPGISGDVDLDYAYKDYSTMIQNAGLNGFTKTMQPTEPEPEPTPTPEPDSEETTLQQILKHVASLDEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5UWR2</accession>
    <name>R5UWR2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Endoglucanase C307</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00568</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000004">
        <property type="protein sequence ID" value="CCZ82763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00568"/>
    <dbReference type="EnsemblBacteria" id="CCZ82763">
        <property type="protein sequence ID" value="CCZ82763"/>
        <property type="gene ID" value="BN566_00568"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071704">
        <property type="term" value="P:organic substance metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cellulase" evidence="3">
        <location>
            <begin position="31"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ82763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="343" mass="39318" checksum="8889D85DF6952075" modified="2013-07-24" version="1">MKTWNGFEKGINLGGWLSQWDLTDREHLETFITEQDIQDIRAMGADHVRLPVDYSLIEEEDGTPKDENFIYIDRCLEWCKKAGLHVLLDLHKTAGYAFDEAKDCASFFESPALQERFLCLWDKLAARYGKYSDFVAFDLLNEIVDPDVAEIWNALALRAVERIRRVTKETDLLIGGARYNSIMSVKDLILPPDEHIVYSFHCYEPILFTHQGAYWVDGMPEDFRIGYPLDTATCKQLSKKMMPVEMSDILDLVASDAQGKDFFLDLFRPAVKIAEERNVALYCGEYGVIDRANPEDTIRWYEAIHAAFTELGIGRAMWTYKALDFGLVGEHYAGVRKDLIALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXC4</accession>
    <name>R5UXC4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase IS116/IS110/IS902 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00753</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000032">
        <property type="protein sequence ID" value="CCZ82953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82953">
        <property type="protein sequence ID" value="CCZ82953"/>
        <property type="gene ID" value="BN566_00753"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003346">
        <property type="entry name" value="Transposase_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002525">
        <property type="entry name" value="Transposase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01548">
        <property type="entry name" value="DEDD_Tnp_IS110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02371">
        <property type="entry name" value="Transposase_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEDD_Tnp_IS110" evidence="2">
        <location>
            <begin position="4"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="237"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ82953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="399" mass="44921" checksum="0A51A9CF0AC72957" modified="2013-07-24" version="1">MFYCGIDIAKYKHEASVIDMSGKALSNSITFANDRQGCEKVLAIFEKSEITPDNIVIGMEATGHYWLSVYTFFLELGYTVKVINPIQSEAFRKMYIRQTKNDSKDSYIIAQIMRFGEYSATSLSEENIIALRQLSRYRLALVDACGDCKRRVIALLDQVFPEYDKLFSDTFGKTSSELLLNCPTPEDMLAVSTRKLTNILNKASRGRFGKEKAEELKDAAKNTFGIAFAKDAFSFQIKQLMQQIIFTENQLSELEEQIANLLHETNQYITTITGIGDVLGAIIVSEIGDINRFERPNQLVAFAGLDVTVKQSGEFSGTKNKISKRGSPYLRRAIWLAAQRAAFCDPVLSDYYQSLKARGKHHLTAVGAVARKLCNIIFVILKENRPYFPTPPKKSQNLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXK1</accession>
    <name>R5UXK1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC-type multidrug transport system ATPase and permease components</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN566_00796</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000038">
        <property type="protein sequence ID" value="CCZ83003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00796"/>
    <dbReference type="EnsemblBacteria" id="CCZ83003">
        <property type="protein sequence ID" value="CCZ83003"/>
        <property type="gene ID" value="BN566_00796"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="PTHR24221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="52"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="133"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="157"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="237"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="20"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="334"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="368"/>
            <end position="375"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ83003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="584" mass="63999" checksum="204A7298923F4369" modified="2013-07-24" version="1">MWKLLRFLKPYRKESICAPLFKLLEVVFELLVPLVVAHMIDSGIGGADKGIIVRSVLLMITLGAVGLASATTAQYFAAKASAGFGANIRYAMMQHIQKRSYAELDQMGTNTMITRITSDVNQVQSGVNMALRLVMRSPFVVIGAMIMAFTVDVKCALIFAAVIPLLTIVVFAIMFGSIPLYQNVQKKLDGILGRTRENLLGVRVLRAFRKEQEEMDAFTQESVELSVLQKRVGRISALMNPATYILLNLAIVFLIWTGALQVNVGVLTQGAIIALYNYMSQILVELIKLANLIITLTKSIASGNRIQDMLEFGEEPNNTGKQDCKVVATAGAEIRFDHVSCRYPQASADALEDISFTAKAGETIGIIGGTGSGKTTLVNLIPNFYPATAGTVFIDGMDVKQCTDASLRAQIGIVPQKAVLFQGTIRENLQWGKEDATDAELYQALEIAQAREIVEKKAGKLDERITENASNLSGGQRQRLTIARALVRQPRILILDDSASALDYATDAALRQSLQQMPNRPTIFLVSQRTASLLQADRILVLDGGKLVGNGTHADLLETCPVYQEIYYSQFPKEEVQTNAEENR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXR7</accession>
    <name>R5UXR7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00895</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000059">
        <property type="protein sequence ID" value="CCZ83108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83108">
        <property type="protein sequence ID" value="CCZ83108"/>
        <property type="gene ID" value="BN566_00895"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="135" mass="14288" checksum="A1CA03406B6A3CCA" modified="2013-07-24" version="1">MPTFELKCPYCESKNVVSKNTKLKYGGVGAATLGVLGALALGPIGLLGGAAIGAFTGAKEAELIKYHCSDCDKDFTVCSKCNGHVEKLAQVGGNACARKQPNGTKYYTPGAKCPLCGYVFRQPRTSFTPKNNNNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXS3</accession>
    <name>R5UXS3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Deoxyribose-phosphate aldolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00905</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000059">
        <property type="protein sequence ID" value="CCZ83118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00905"/>
    <dbReference type="EnsemblBacteria" id="CCZ83118">
        <property type="protein sequence ID" value="CCZ83118"/>
        <property type="gene ID" value="BN566_00905"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="120" mass="13747" checksum="3D2463AAF8A35BC9" modified="2013-07-24" version="1">MTKSQKNSVDEMLAVGISIPKIAAFLHVSQNSIKSYLQRNHPNDVCRNCGTPVLQVPHRKQKKFCCDACRMHYWNTHPQEMRHENAATIPCAFCGKPVLSYRNHPRKYCSRACAAKGRYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXZ3</accession>
    <name>R5UXZ3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RNA polymerase sigma factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN566_00735</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the sigma-70 factor family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000029">
        <property type="protein sequence ID" value="CCZ82935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00735"/>
    <dbReference type="EnsemblBacteria" id="CCZ82935">
        <property type="protein sequence ID" value="CCZ82935"/>
        <property type="gene ID" value="BN566_00735"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030435">
        <property type="term" value="P:sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014322">
        <property type="entry name" value="RNA_pol_sigma-B/F/G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000943">
        <property type="entry name" value="RNA_pol_sigma70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007624">
        <property type="entry name" value="RNA_pol_sigma70_r3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007630">
        <property type="entry name" value="RNA_pol_sigma70_r4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04539">
        <property type="entry name" value="Sigma70_r3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04545">
        <property type="entry name" value="Sigma70_r4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00046">
        <property type="entry name" value="SIGMA70FCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02980">
        <property type="entry name" value="SigBFG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00715">
        <property type="entry name" value="SIGMA70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00716">
        <property type="entry name" value="SIGMA70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="3" id="KW-0749">Sporulation</keyword>
    <keyword evidence="7 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="9">
        <location>
            <begin position="217"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ82935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="258" mass="29210" checksum="E51E842E4FBEB0B0" modified="2013-07-24" version="1">MAFQKVMISGIHTSELTVLKEEEKMALLREIQETGSKAARDKLIQGNLRLVLSVIQRFTGRGEDMEDLFQVGVVGLIKAINHFDLSKEVRFSTYCVPMISGELRRYLRDFNQVKVSRSLRDLAYKAMQAKERLTGTLQREPTIEQIATEIGAKREDVVIALESITDPVSLYEPVYSDSGDTLYVMDQISDRDSMENWLGELSIREAMQGLGQREKNILYLRFFRGKTQMEVAGEIGISQAQVSRLEKGALLKIKAAVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5UY17</accession>
    <name>R5UY17_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01006</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000076">
        <property type="protein sequence ID" value="CCZ83223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01006"/>
    <dbReference type="EnsemblBacteria" id="CCZ83223">
        <property type="protein sequence ID" value="CCZ83223"/>
        <property type="gene ID" value="BN566_01006"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025964">
        <property type="entry name" value="GGGtGRT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14057">
        <property type="entry name" value="GGGtGRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="339" mass="35975" checksum="9138ADFFFB3274DC" modified="2013-07-24" version="1">MALFESYERREKQILAVLKEYGIDSIEGAAEVTKAAGLDVYKMVEGIQPICFENAKWAYTVGAAIAIKKGCRRAADAAAAIGEGLQAFCIPGSVADQRKVGLGHGNLGKMLLEEETKCFAFLAGHESFAAAEGAIGIAEKANKVRKEPLRVILNGLGKDAAQIIARVNGFTYVETEMDYYTGEVKEVFRKCYSKDPESPRAKVNCYGANDVTEGVAIMWKEGVDVSITGNSTNPTRFQHPVAGTYKKECIDAGKKYFSVASGGGTGRTLHPDNMAAGPASYGMTDTMGRMHSDAQFAGSSSVPAHVEMMGLIGMGNNPMVGATVAVAVSVEEAAKAGKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5UY54</accession>
    <name>R5UY54_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Membrane associated protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01039</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83258">
        <property type="protein sequence ID" value="CCZ83258"/>
        <property type="gene ID" value="BN566_01039"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038690">
        <property type="entry name" value="NusG_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024045">
        <property type="entry name" value="NusG_dom2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07009">
        <property type="entry name" value="NusG_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="NusG_II" evidence="2">
        <location>
            <begin position="16"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="137" mass="15124" checksum="F7BAC68C7EE5E282" modified="2013-07-24" version="1">MTKIQIRKQQILVIGIFLFLLLAGILGSYFVLHRSIPHRVVVISQDGKEVQRIAMDTVKNPHIIEITGEDGAWNRVWISEDSVKMESASCPDGLCVHQGAISDGLLPIVCLPNKVQIQIVEDTGESSETEEFDAKVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYI1</accession>
    <name>R5UYI1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00873</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000051">
        <property type="protein sequence ID" value="CCZ83085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83085">
        <property type="protein sequence ID" value="CCZ83085"/>
        <property type="gene ID" value="BN566_00873"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021377">
        <property type="entry name" value="DUF3006"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11213">
        <property type="entry name" value="DUF3006"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="71" mass="8275" checksum="776E9CEC73168348" modified="2013-07-24" version="1">MIIIDRMDDGVAVLETDTGMQTVSADQMPEGARDGDVLQWVHDHYEVDTAATEERRNRMLERMRKRKRGRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZ21</accession>
    <name>R5UZ21_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01081</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01081"/>
    <dbReference type="EnsemblBacteria" id="CCZ83300">
        <property type="protein sequence ID" value="CCZ83300"/>
        <property type="gene ID" value="BN566_01081"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="215"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="247"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="324"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="355"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="383"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="552" mass="63116" checksum="CA981D86A44439D7" modified="2013-07-24" version="1">MTKTLYFSNACKKLAQKKQTLQPFLWTLFSLILLVGSASTVLYYIFAVAINEFHSDCTDTLYWAEAAMQGNGLINPDFTYAALMPLGGNLFMQIWIPLFGISMKTQAFGMATFFVTFYVFLCLMLREMHFSLRATAVTVSVLLLTLCSSVKLREIFWNHIIYYSIGILFLVIGLFFVLHIRNLSERRQTKSVKIQTVIFYVLLAADFIVCCTNSTTSIALFALPILGGVFCERFLDIRTPWKSRKSVTALLTLLICAIGLYLGMKLGAHIDGDVQEGYASAYSRFSNPDTWWDYVEALPLAFLQLLGLNVQESDLLASIEGVRAILTIFYALFLAIVPIIALCCYAKIEEAGVRVLIWAHWVVTAFILVGYLCGLLSAGIWRLSPIVCTSVLVSLAFLRWLYHKVETRRWCVLLCLPLAYLCLHDVHKVIEIPVNDYKESINYKLGEYLKDQNLTYGYASFWHSQAITVLEDSDVKIRTVNFTDDERGLEAGLYQSNKNWFEDQPEQDRYFLLMQQDEKEKMEQSTSSILTLPHEEQTYEGYTIWIFNENIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZ30</accession>
    <name>R5UZ30_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA mismatch repair protein MutL</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">mutL</name>
        <name evidence="8" type="ORF">BN566_01331</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">This protein is involved in the repair of mismatches in DNA. It is required for dam-dependent methyl-directed DNA mismatch repair. May act as a 'molecular matchmaker', a protein that promotes the formation of a stable complex between two or more DNA-binding proteins in an ATP-dependent manner without itself being part of a final effector complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the DNA mismatch repair MutL/HexB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000131">
        <property type="protein sequence ID" value="CCZ83563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01331"/>
    <dbReference type="EnsemblBacteria" id="CCZ83563">
        <property type="protein sequence ID" value="CCZ83563"/>
        <property type="gene ID" value="BN566_01331"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032300">
        <property type="term" value="C:mismatch repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00149">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014762">
        <property type="entry name" value="DNA_mismatch_repair_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020667">
        <property type="entry name" value="DNA_mismatch_repair_MutL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002099">
        <property type="entry name" value="DNA_mismatch_repair_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013507">
        <property type="entry name" value="DNA_mismatch_S5_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038973">
        <property type="entry name" value="MutL/Mlh/Pms"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014790">
        <property type="entry name" value="MutL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042120">
        <property type="entry name" value="MutL_C_dimsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042121">
        <property type="entry name" value="MutL_C_regsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037198">
        <property type="entry name" value="MutL_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073">
        <property type="entry name" value="PTHR10073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01119">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08676">
        <property type="entry name" value="MutL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01340">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00853">
        <property type="entry name" value="MutL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118116">
        <property type="entry name" value="SSF118116"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00585">
        <property type="entry name" value="mutl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00058">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3 4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA_mis_repair" evidence="7">
        <location>
            <begin position="210"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="MutL_C" evidence="6">
        <location>
            <begin position="474"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="349"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="659" mass="73996" checksum="2335BDE6CCE04FB1" modified="2013-07-24" version="1">MSKIAVLSKEISELIAAGEVIERPASVVKELIENAIDAEAKHITVEIKHGGTTYMRVADDGSGIASEDVPTAFFRHATSKIHDRKDLESIYTLGFRGEALASIAAVSKVTLLTKRREDDYGTHYEIIGGTPTGEPEQGGCPDGTTILIRDLFFNVPVRQRFMKKDVTEANAVSQIVQKIALSHPEISFQMIRDNRMEFCTDGNGDLYAAIYALFGKEFAHDLIPVQYDDGFLRVSGYVGKPLYSKSNRTFQHFFINGRYIRSRVCSVALESAYQNLIMTGKFPTCVLMLEMLPVDLDVNVHPAKAEVRFTSEKNVADSLFFAVKNALLENGLIYDFQFRHNAQDWTKQPAVSTPMQQPTLPHLKEPSDPFASKAEPEVESPPPPQTIYPTQYHYDIGETMAALMPEETRPAALENPQAEHTKSELELEQNAELEPQSEATALPETILPLPTATPEPSVPESLPEKQTILPEIRVIGEVFQNYILAEIPAEQKLVIFDKHAAHERVIFERLKSGAAKQYQQLLMQKTETLLPMDEFALMQEQTEQLENMGFSFDFSNPPFLRTTAVPTFLQSLNIDEIVTEIAHNFALGKINPQTAYLDDLLHTMACKAAIKAHDKNDLAELQELAQIVYADEQVRHCPHGRPVMFVIKKHELEKQFKRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZA8</accession>
    <name>R5UZA8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA polymerase III alpha subunit (Gram-positive type)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01146</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000093">
        <property type="protein sequence ID" value="CCZ83370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01146"/>
    <dbReference type="EnsemblBacteria" id="CCZ83370">
        <property type="protein sequence ID" value="CCZ83370"/>
        <property type="gene ID" value="BN566_01146"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Exonuclease_RNaseT/DNA_pol3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exonuclease" evidence="1">
        <location>
            <begin position="3"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="176" mass="20011" checksum="8E3904599F7E514E" modified="2013-07-24" version="1">MAVYIVFDVETPNRANDRMSAIGISAIKDNKIVANYFSLVDPETHFDYFNTKLTGIDADKVKGAPNFAELWKKIEPIMSKGILVAHNAVFDMSVLRACLRDYGISWKPTAKYLCTVRIGRRVLPDMKHNLNVMCDHYGIALDHHQADSDSHACAEILLRYIESGIDVREYVKEYKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZA9</accession>
    <name>R5UZA9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HK97 family phage prohead protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01409</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000148">
        <property type="protein sequence ID" value="CCZ83673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01409"/>
    <dbReference type="EnsemblBacteria" id="CCZ83673">
        <property type="protein sequence ID" value="CCZ83673"/>
        <property type="gene ID" value="BN566_01409"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006433">
        <property type="entry name" value="Prohead_protease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04586">
        <property type="entry name" value="Peptidase_S78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01543">
        <property type="entry name" value="proheadase_HK97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-1188">Viral release from host cell</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="193" mass="21997" checksum="A5B21DFCC6BA6874" modified="2013-07-24" version="1">MERNNVMYRTMQSVLTTRDGETNEAPVIEGYFAVFDSNYDMGYGMSESVAPGAFSETLSGDVRALIDHDTRLVLGRTTAHTLELREDSHGLWGKIYINPKDSEAMNLYERVKRGDVSQCSFGFEILSEETTFPADGEIHWRITKVKLYEVSCCTYPAYEETGISARKKDREQIEKRKSEAWKSALLKKLKGEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZE8</accession>
    <name>R5UZE8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in bacteria</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01176</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000095">
        <property type="protein sequence ID" value="CCZ83400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01176"/>
    <dbReference type="EnsemblBacteria" id="CCZ83400">
        <property type="protein sequence ID" value="CCZ83400"/>
        <property type="gene ID" value="BN566_01176"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009296">
        <property type="entry name" value="DUF951"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38455">
        <property type="entry name" value="PTHR38455"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06107">
        <property type="entry name" value="DUF951"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="131" mass="15001" checksum="EC8C26518B9F23F0" modified="2013-07-24" version="1">MEECPVFAGHFYYFSTNAKKYSLKMLPYAKKSGIIEVLMCNAPSEMDEQSNAMGEETIMDVRPADLLLMRKPHPCGGNTFYVIRSGMDFKLRCTTCGREFMIPRNKIERRIKQVIRETDSSESTNTLPTSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZI1</accession>
    <name>R5UZI1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Radical SAM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_01493</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000161">
        <property type="protein sequence ID" value="CCZ83758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01493"/>
    <dbReference type="EnsemblBacteria" id="CCZ83758">
        <property type="protein sequence ID" value="CCZ83758"/>
        <property type="gene ID" value="BN566_01493"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039661">
        <property type="entry name" value="ELP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032432">
        <property type="entry name" value="Radical_SAM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135">
        <property type="entry name" value="PTHR11135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16199">
        <property type="entry name" value="Radical_SAM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="2"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="346" mass="38258" checksum="FB038FB9DEC653EF" modified="2013-07-24" version="1">MKNKGNIAIFVPHAGCPHQCSFCDQRKISAAQQIPTLEQVQEICRQAIAHPRRPKQLEIAFFGGSFTAIPEKEQNALLEAVQPFLAAEGVTGIRISTRPDCITPEILQRLQQFHVTAIELGAQSMQDAVLQKNHRGHTAQDVRNAAKWITAADFSLGLQMMIGLDGSTPEQERKTLQELLALHPDTLRIYPLVVLKGTELAERVQNGSFVLYDWETVLELCADALCACRQQGVRVIRCGLHAEDTVQSEAVAGFYHPAFRELVESRLCLRVLKQWMQQHPQETMAEVQVAPGWSSRVAGHHACNRAACREAGVSLRIIETAAIPAGMLQIGKDGYDVFQIAGTARI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZJ3</accession>
    <name>R5UZJ3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7 8">DNA replication and repair protein RecF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">recF</name>
        <name evidence="10" type="ORF">BN566_01209</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 8">The RecF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the RecF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000104">
        <property type="protein sequence ID" value="CCZ83435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01209"/>
    <dbReference type="EnsemblBacteria" id="CCZ83435">
        <property type="protein sequence ID" value="CCZ83435"/>
        <property type="gene ID" value="BN566_01209"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00365">
        <property type="entry name" value="RecF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001238">
        <property type="entry name" value="DNA-binding_RecF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018078">
        <property type="entry name" value="DNA-binding_RecF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042174">
        <property type="entry name" value="RecF_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32182:SF0">
        <property type="entry name" value="PTHR32182:SF0"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00611">
        <property type="entry name" value="recf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00617">
        <property type="entry name" value="RECF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00618">
        <property type="entry name" value="RECF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7 8" id="KW-0235">DNA replication</keyword>
    <keyword evidence="7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7 8" id="KW-0742">SOS response</keyword>
    <feature type="domain" description="SMC_N" evidence="9">
        <location>
            <begin position="3"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="30"/>
            <end position="37"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ83435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="372" mass="41547" checksum="4B540B6BAF515944" modified="2013-07-24" version="1">MRLTSVAADGFKNLKAVQFSPSPHYNLIIGENAQGKTNLLEAIWMMTGCRSFRGTRERDYLCTDGSPLHVAIAFDDGRREQVLTCDRTSFKDRTFTCNGIPIEKMGDLFHAFHCIAFTPEDLALVTGTPDVRRQFLDLSLSQLQPHCLELVRRYQLILSHRNAVLRQPVAILERAAALDVWDVQLAAVGAEIAAQRACYVQQLQKTGEPLYQEIAAQKERLQIAYHANIYGVAPDLPEHADAAMQEQYQKRLLAARETDLQLGFTSVGVHRDELTFQLNGQPARTFGSQGQKKSLALALRLSQAALYAKRYQASPILLLDDVMGELDAGRQQVVCTIIQDMQVFLTTCHAESLIAAVDGERLYLKNGQMQTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZJ6</accession>
    <name>R5UZJ6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Preprotein translocase YajC subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN566_01513</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YajC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000161">
        <property type="protein sequence ID" value="CCZ83778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01513"/>
    <dbReference type="EnsemblBacteria" id="CCZ83778">
        <property type="protein sequence ID" value="CCZ83778"/>
        <property type="gene ID" value="BN566_01513"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003849">
        <property type="entry name" value="Preprotein_translocase_YajC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33909">
        <property type="entry name" value="PTHR33909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02699">
        <property type="entry name" value="YajC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01853">
        <property type="entry name" value="YAJCTRNLCASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01323">
        <property type="entry name" value="YajC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00739">
        <property type="entry name" value="yajC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004394833" evidence="10">
        <location>
            <begin position="29"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="130"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ83778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="162" mass="17591" checksum="B9E538EA000F9B98" modified="2013-07-24" version="1">MRKRMAKYGLAVSVAALTGALSMMPAAASSGSSSSSSTGSGGMIGSLIMMVLLFAVMYFILIRPQKKKEKEQQAMQRAVRVGDEIVTIGGIVGLVTRVEDDTIQIETGGDRHKMRIKTWAIQENITVKEDNERAKAAAAEEAKAKKESKKKKKDDEESVLKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZW9</accession>
    <name>R5UZW9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Glycoside hydrolase family 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN566_01284</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000119">
        <property type="protein sequence ID" value="CCZ83515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01284"/>
    <dbReference type="EnsemblBacteria" id="CCZ83515">
        <property type="protein sequence ID" value="CCZ83515"/>
        <property type="gene ID" value="BN566_01284"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008810">
        <property type="term" value="F:cellulase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02850">
        <property type="entry name" value="E_set_Cellulase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004197">
        <property type="entry name" value="Cellulase_Ig-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002105">
        <property type="entry name" value="Dockerin_1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001701">
        <property type="entry name" value="Glyco_hydro_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02927">
        <property type="entry name" value="CelD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00404">
        <property type="entry name" value="Dockerin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00759">
        <property type="entry name" value="Glyco_hydro_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="SSF48208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="SSF81296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004394845" evidence="7">
        <location>
            <begin position="30"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="8">
        <location>
            <begin position="784"/>
            <end position="844"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ83515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="844" mass="94719" checksum="F62B43A41C6A377D" modified="2013-07-24" version="1">MKFFSKKRWMAGLLSVVLCSSVLTANAWAANVEETPGLQLQTILIDNDGKNWTTESWGGATMTPNTNWTTLNMQDYYEHGMLHFAVKNNGTGTTTFRIGLVSHHHNQTIKIEWSSLEQYGKLNAETNWTTYSLPIKTLVDAYPDSDFRLDNFWYVYVGGVSSDTTLSFQNVKITSTDDERQYPMIKVNQVGYFSNGAKTARVSYFEKFGSLDGKTYEIVDAEQGNVVATGTLPTAQKEETLSGEMVHTISFDAVTEPGSYYIRIPDAGLDASARSPQDVADGLDTDTILSPTFSIENHVYDALFSDMTKYFYYQRQGIDLEETYAGVFARENLHPNDVTVKKWSDRDNPNAETYDVSGGWYDAGDYGKYVSPAAGTVEDLLLAYELFPDTFRNMDLNIPETDPNNARYVDAPGMLSELKWELDMLLKLEHSDKDGSFYVAANYKDDVIYLEDTLRSTDTYQSDDSAKDLRSHLATADAAAIFAHAYLVYREIPAYADFADTCLETALRAWNWVTDPSNPKHMSIGAANRTYTFTQEEFDRDLFWAAGSLYRAVKTAGGDVSPYENYLLANCNTDTVQNCFKNISLSYNHAGESFLGFFHYLYQNEQPNAAMTEAFSNFTPWRTNMLQHNNWGMVFPNWGYWWGSNRNVAQNAMTLLLGSVILEGQDNIPTAVSEAADHAFDYLLGDNPISFSYVSGYGERSVENIYSKIYSVDAALTPYQVPKGYVTEGTNYHNNRHLSKFDGKCYMDSDTEYTTNENTIYGNASALFLTAAVIAGHTEPDPEPDTVQGDVNADGTFDLADVVMLQKWLIRAGTLTDWAAGDWNDDETITVVDLCLMKRALQQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5V050</accession>
    <name>R5V050_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00147</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000146">
        <property type="protein sequence ID" value="CCZ83620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00147"/>
    <dbReference type="EnsemblBacteria" id="CCZ83620">
        <property type="protein sequence ID" value="CCZ83620"/>
        <property type="gene ID" value="BN566_00147"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004069">
        <property type="term" value="F:L-aspartate:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024551">
        <property type="entry name" value="AspAT_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12897">
        <property type="entry name" value="Asp_aminotransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="424" mass="47057" checksum="B1983EF99C6E0FB9" modified="2013-07-24" version="1">MRLQEKNHAELEAFQAECQQAYKQFQAENLKLDMSRGKPAPAQLELSMDMMDCLKKGDYRAENGLDCRNYGVLDGLPETKAFFAPMLGVKPEEIIVYGNSSLNIMYWVMSVAMTNGVLGGTPWGKLDHVKFLCPVPGYDRHFAVTQFFGMELINVPMNEDGPDMDIVEKLVAEDDSIKGIWCVPMYSNPGGVVYSDEVVKRFAALKPKAADFRIFWDNAYCVHHLVDNPPVQANLLEEAKKVGNEDICYMFASTSKITFPGSGIAVMAASERNLASLKKSLGFATIGFDKMNQLRHLRFFGGKFENLLEHMKKHKALIAPKFAIVVNTLEKELGDLDIATWSNPKGGYFISFNQKGCAKRIVQLCKDAGVVLTGAGASFPYGVDPEDENIRISPTFPTEEELQKAMDVFVCSAKLAAAEQLLAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0Q8</accession>
    <name>R5V0Q8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC-type multidrug transport system ATPase component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_01908</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01908"/>
    <dbReference type="EnsemblBacteria" id="CCZ84183">
        <property type="protein sequence ID" value="CCZ84183"/>
        <property type="gene ID" value="BN566_01908"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="2"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="310"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="323"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ84183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="350" mass="38760" checksum="EE5EA2DA374311D8" modified="2013-07-24" version="1">MVEIKNLTKYYGQKAAVKNISFTVEDHEILGFLGPNGAGKSTTMNIICGVIPSSSGTVTINGFDIAKDPIKAKRHIGFLPEIPPVYPDMKVKEYLTFVAGLKGVPGVKRKAQVAKAMDRLRITDVAGRLIRNLSKGYRQRVGFAQALLGEPDVLILDEPTVGLDPEQVIEVRNLILELKQDHTIILSSHVLSEISAVCDRVVIISKGEIRAVDTIDHLEERADTNPILHLKVKGGSQEVAATMRQVDGVRNVSNISRFKTGIYTYDVELQSSDIRNSLLAALLVKKFDVLEVKEQKKNLEQVFVKLVNQSSSSEQRSSMQALLKELEADDTEQESTDKQDDSNHHPHTDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0X8</accession>
    <name>R5V0X8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01654</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000173">
        <property type="protein sequence ID" value="CCZ83925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83925">
        <property type="protein sequence ID" value="CCZ83925"/>
        <property type="gene ID" value="BN566_01654"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="194" mass="21607" checksum="A2497DE9BB044E4C" modified="2013-07-24" version="1">MKTSLKTTIQTFCHDLSAKTRITVLCCGCFLVLTGFLLVFLMLCPVKKENLSNHANENLMSAITTSAPTATTTAEETTTFKRTRKTTDKKRARSTTQTTVKTELIETEEPLMTEENSLDDLYSNDYNNNYNYNYGYDNSYNNGYDNSYNNGYDNGYNNGNGYYEPATDAPNYDSIPDPEPVPSEGDAGGSDLGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0Y8</accession>
    <name>R5V0Y8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BATS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_00197</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000204">
        <property type="protein sequence ID" value="CCZ84268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00197"/>
    <dbReference type="EnsemblBacteria" id="CCZ84268">
        <property type="protein sequence ID" value="CCZ84268"/>
        <property type="gene ID" value="BN566_00197"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044271">
        <property type="term" value="P:cellular nitrogen compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018130">
        <property type="term" value="P:heterocycle biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901362">
        <property type="term" value="P:organic cyclic compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901566">
        <property type="term" value="P:organonitrogen compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042364">
        <property type="term" value="P:water-soluble vitamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010722">
        <property type="entry name" value="BATS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024007">
        <property type="entry name" value="FeFe-hyd_mat_HydG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034428">
        <property type="entry name" value="ThiH/NoCL/HydG-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43583">
        <property type="entry name" value="PTHR43583"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43583:SF2">
        <property type="entry name" value="PTHR43583:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06968">
        <property type="entry name" value="BATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00319">
        <property type="entry name" value="Fe_hydrogenase_maturase_(HydG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00876">
        <property type="entry name" value="BATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03955">
        <property type="entry name" value="rSAM_HydG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="BATS" evidence="6">
        <location>
            <begin position="275"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ84268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="472" mass="53338" checksum="8E6899C703E8DE02" modified="2013-07-24" version="1">MYLPNSTKAAEFLCHEEVMQTLAYAQENKNNLPLIQEILSKARLQKGLTHREASVLLACPSETVWEELYQLAEAIKLDYYGKRIVLFAPLYLSNYCVNSCVYCPYHAKNKHICRKKLTQEEIKREVIALQDMGHKRLALEAGEDPLHNPIEYILESIDTVYHIQHKNGAIRRVNVNIAATTIQNYRKLHDAGIGTYILFQETYHKEQYEKLHPAGPKHNYDYHTEAMDRAMQGGIDDVGLGVLFGLQTYPYELAGLLMHAEHLEATYGVGPHTISVPRIKHADDIDPNAFDNSIDDETFAKICTVIRIAVPYTGMIMSTREGKAVRERALQLGISQVSGASCTSVGGYDTPEPESENSAQFDVSDRRTLDEVVRWLMELGYIPSFCTACYRAGRTGDRFMSLCKSGQIQNCCHPNALMTLNEYLEDYAKPETRAIGKALIQKELLNIPNEKVRAIAAEHIAEMAAGKRDFRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1A6</accession>
    <name>R5V1A6_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">1,4-alpha-glucan branching enzyme</fullName>
            <ecNumber evidence="5">2.4.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BN566_00275</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Transfers a segment of a (1-&gt;4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.</text>
            <dbReference type="EC" id="2.4.1.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Glycan biosynthesis; glycogen biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.18" evidence="5"/>
    <dbReference type="EMBL" id="CBAR010000221">
        <property type="protein sequence ID" value="CCZ84368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00275"/>
    <dbReference type="EnsemblBacteria" id="CCZ84368">
        <property type="protein sequence ID" value="CCZ84368"/>
        <property type="gene ID" value="BN566_00275"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00164"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003844">
        <property type="term" value="F:1,4-alpha-glucan branching enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102752">
        <property type="term" value="F:1,4-alpha-glucan branching enzyme activity (using a glucosylated glycogenin as primer for glycogen synthesis)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043169">
        <property type="term" value="F:cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd02855">
        <property type="entry name" value="E_set_GBE_prok_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006048">
        <property type="entry name" value="A-amylase/branching_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037439">
        <property type="entry name" value="Branching_enzy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006407">
        <property type="entry name" value="GlgB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044143">
        <property type="entry name" value="GlgB_N_E_set_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="Glyco_hydro_13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004193">
        <property type="entry name" value="Glyco_hydro_13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43651">
        <property type="entry name" value="PTHR43651"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02806">
        <property type="entry name" value="Alpha-amylase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02922">
        <property type="entry name" value="CBM_48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000463">
        <property type="entry name" value="GlgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01515">
        <property type="entry name" value="branching_enzym"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="9" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="10" id="KW-0320">Glycogen biosynthesis</keyword>
    <keyword evidence="6" id="KW-0321">Glycogen metabolism</keyword>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aamy" evidence="14">
        <location>
            <begin position="140"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="611"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="12">
        <location>
            <position position="299"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="12">
        <location>
            <position position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000463-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CCZ84368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="639" mass="74022" checksum="99DC3BB0B2352C8E" modified="2013-07-24" version="1">MDIHQFFLGNAFDAYTFFGAHVQKDGSVCFRTLAPNAEKVSLIYEGNDWKEIPMEKTHPGGVYTCIVPDAIPGMMYKYRIYSEHFADVEFIDHCDPYGFGMELRPGTASIIRDTDSYHFQDSEWLSQRGGHLEKPVNIYEVHLGSWKTNPNDPNGWYRYEELAPLLIAYAKKNGYNYIECMPLSEHPADCSWGYQNTGFYSPTARYGDMDGLKKFVDLCHRSGIGVIMDFVPVHFAVDGYGLARYDGTALYEYPDDNSDNYSEWGSCNFIHARGEVCSFLQSCANYWISEFHFDGIRMDAVSRLIYWGGDPGRGENEASLNFLKRMNQGLHQLHPDAMLIAEDSTDFDGVTRPVEFGGLGFDYKWDMGWMNDTLNYFRSNPFFRGSGYHQITFSMMYYYKERYLLPFSHDETVHGKATIVQKMFGEYEQKFPQARALYLYMYTHPGKKLNFMGNELGQLREWTEEQEQDWDILKFPIHDAFHQYMQDLNKLYLQEPALSAWDYRREGFRWIDCHQENRCIYAYERRANRERLLIILHFSDLQNQQYTVTVPDCAALEPLLHSDWACYHGSVEKNTDLILTTPTEKGGQVTLPLAPYSAMLFRVHTAVEKSVSAPPVQAKKPRKPRTSTRKTCKTAETAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1D3</accession>
    <name>R5V1D3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glutamyl-tRNA(Gln) amidotransferase subunit A</fullName>
            <shortName evidence="8">Glu-ADT subunit A</shortName>
            <ecNumber evidence="8">6.3.5.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">gatA</name>
        <name evidence="10" type="ORF">BN566_01776</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 8">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) + L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterotrimer of A, B and C subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the amidase family. GatA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.7" evidence="8"/>
    <dbReference type="EMBL" id="CBAR010000182">
        <property type="protein sequence ID" value="CCZ84050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01776"/>
    <dbReference type="EnsemblBacteria" id="CCZ84050">
        <property type="protein sequence ID" value="CCZ84050"/>
        <property type="gene ID" value="BN566_01776"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030956">
        <property type="term" value="C:glutamyl-tRNA(Gln) amidotransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00120">
        <property type="entry name" value="GatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000120">
        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004412">
        <property type="entry name" value="GatA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895">
        <property type="entry name" value="PTHR11895"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="SSF75304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00132">
        <property type="entry name" value="gatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Amidase" evidence="9">
        <location>
            <begin position="19"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8">
        <location>
            <position position="73"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8">
        <location>
            <position position="148"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="8">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ84050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="480" mass="51605" checksum="8F2BD5734EF44052" modified="2013-07-24" version="1">MGIREQRNLLDRHEISVKELTECYLNRIETYDPALQSYITVTKEQAYQDAETAQKKIDDGTAAPLCGIPLAIKDNICTDGVRTTCASKMLEDFIPPYNATVMEKLNAQNAVMLGKTSMDEFAMGGSTQTSAFAKTKNPFGKDCVPGGSSGGSAACVSASLATAALGSDTGGSIRQPSAFCGVTGLKPTYGRVSRYGLIAFASSLDQIGPIAKSPYDCALLLNAIAGYDPHDGTVSKKPVEDYTAKIGKDIKGMKIALPEEFFGEGIDDSIRNAVMEAAKTYEKMGATLVSCNMPSLKYAVAAYYLIASAEASSNLSRYDGIKYGHRSKVGENFNELICNSRSEGFGSEVKRRILLGNYALSSGYYDAYYGKAMALRQKISAEYAAIFEKCDVMLTPTTPTVAYHIHENISDPVKMYQADICTVTANIAALPSLSTPCGYDEAGMPIGMSLTGRQFDEATILQVADAFSQQFTLRTPSLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1N4</accession>
    <name>R5V1N4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01910</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01910"/>
    <dbReference type="EnsemblBacteria" id="CCZ84185">
        <property type="protein sequence ID" value="CCZ84185"/>
        <property type="gene ID" value="BN566_01910"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="24"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="121" mass="14018" checksum="D041BB0CDCE609BB" modified="2013-07-24" version="1">MAMANIFEQMSRSVSGMSKNASEASNLRKKIAYERERIQEVMQEIGELYYESQEKELAKLKELCADIDDRKRRIQSMRLELQQMKGKRVCPGCGMQFDEKYQFAFCGKCGAKLDDPITDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1N6</accession>
    <name>R5V1N6_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Beta-glucosidase</fullName>
            <ecNumber evidence="3 11">3.2.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BN566_01915</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.</text>
            <dbReference type="EC" id="3.2.1.21"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the glycosyl hydrolase 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.21" evidence="3 11"/>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01915"/>
    <dbReference type="EnsemblBacteria" id="CCZ84190">
        <property type="protein sequence ID" value="CCZ84190"/>
        <property type="gene ID" value="BN566_01915"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102483">
        <property type="term" value="F:scopolin beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001360">
        <property type="entry name" value="Glyco_hydro_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018120">
        <property type="entry name" value="Glyco_hydro_1_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017736">
        <property type="entry name" value="Glyco_hydro_1_beta-glucosidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033132">
        <property type="entry name" value="Glyco_hydro_1_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353">
        <property type="entry name" value="PTHR10353"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00232">
        <property type="entry name" value="Glyco_hydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00131">
        <property type="entry name" value="GLHYDRLASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03356">
        <property type="entry name" value="BGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00572">
        <property type="entry name" value="GLYCOSYL_HYDROL_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00653">
        <property type="entry name" value="GLYCOSYL_HYDROL_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="6 11" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="9">
        <location>
            <begin position="407"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8 10">
        <location>
            <position position="353"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="400"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR617736-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR617736-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CCZ84190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="449" mass="51693" checksum="B3AB199CAADB7FC9" modified="2013-07-24" version="1">MQFSNDFLWGAASASYQIEGAYLEDGKGENIWDAYCQESGHVAHGENGNVACDHYHLFREDVKMMQKMGIKNYRFSISWTRILPDGTGAVNQKGIQFYSDLVDALLEAGITPMVTLFHWDYPYALHQKGGWLNPDSSDWFVDYAKVVVDALSDRVTYWMTINEPQVFIGCGYAIGKFAPFQKLPARDLAQMSHNVLLAHGKTVKMIRECAKKTPKIGFAFSTPCTTPTDNSPLAIEIARQKSFAFTRERFTFETAWWADPIFLGDYPQDAYSILKSDMPNIKEGDMELISQPVDFYGVNIYYSQAEENPNAYVENDYMGCPRTQMGWPVTPEALYWGPKFLFERYGKPILISENGMASHDWVQLDGKVHDPGRIDYMKRYLRELHRAMQDGTEVMGYMYWSIMDNFEWADGYDKRFGLVYVDYRTQERTIKDSGYFYQTVIETNGETIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5V200</accession>
    <name>R5V200_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ComEC/Rec2-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN566_00419</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000244">
        <property type="protein sequence ID" value="CCZ84558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00419"/>
    <dbReference type="EnsemblBacteria" id="CCZ84558">
        <property type="protein sequence ID" value="CCZ84558"/>
        <property type="gene ID" value="BN566_00419"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004477">
        <property type="entry name" value="ComEC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025405">
        <property type="entry name" value="DUF4131"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03772">
        <property type="entry name" value="Competence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13567">
        <property type="entry name" value="DUF4131"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00360">
        <property type="entry name" value="ComEC_N-term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="238"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="267"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="290"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="314"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="336"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="366"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="400"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="425"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="460"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="485"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4131" evidence="8">
        <location>
            <begin position="31"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="Competence" evidence="7">
        <location>
            <begin position="217"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ84558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="701" mass="77332" checksum="F6FEE4CC144CB079" modified="2013-07-24" version="1">MISTNKKKMKQKMGYLSVAAAAGLFFASSFPLRFFWCGLLFVGGFLWLLYGKHRLSRCAVIVLLAAFTLSGSWYYGYTKLVYDPVISYAGQETTFTGIVTHQTIFNNDKASYQVKGTFPDGTSATILCYTNDYGCNYGDILTLSGTMEIPSSNYLFDGTAYYKAKSIFLQASSDAIVTHTPTAGYSLHRSIEQLRERLQKKILHFAGTTDGSIMIAMLFGQKQLLNDQIKQAFLRSGIGHVLSVSGFHMVVLLMPLAYLGRYRLTRFLRLLLTGGLIGLFALLTESPISILRAGLMVLLAQSGTIFYRKSSTGNALAIAFLVLALPQPYLIRDISFLLSVSGTFGVGVFAPYLTRSLHPKTIFGRLGKQILSMALVFCCTLPVSSWFFSETSLLSPLTNVFLMPFCSLILICTVLTVLTGGVLATFFAGMAMVCCEWVTKAVLWLEKSLPLRFSNSENTFPMLLLGLSLVVFLIWCYLPRKKLLCGLLCGQLAVLTVVQVGMLGVQRNTLFLSVLGRRAEAVVIVQYGNMVDVFDLTGHAKNPQYVQTYLQKHGISHVETLFLLQNVPQMEAAYDTTLSSVSCSAVLIPTGSFCRDEDTVCGQHPQQAERFKVSANTRNAYEIVWDSSALQVTCAGRTITISLKAYDTAIREAQQADFTLLAVHQEWMLTEQRGTELAALLQSDDIQIALSPQGQIRAKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5V208</accession>
    <name>R5V208_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Mannose-1-phosphate guanylyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00429</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000246">
        <property type="protein sequence ID" value="CCZ84568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00429"/>
    <dbReference type="EnsemblBacteria" id="CCZ84568">
        <property type="protein sequence ID" value="CCZ84568"/>
        <property type="gene ID" value="BN566_00429"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005976">
        <property type="term" value="P:polysaccharide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001538">
        <property type="entry name" value="Man6P_isomerase-2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01050">
        <property type="entry name" value="MannoseP_isomer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NTP_transferase" evidence="1">
        <location>
            <begin position="5"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="MannoseP_isomer" evidence="2">
        <location>
            <begin position="317"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="358" mass="39977" checksum="ECAF9772B3EDF83D" modified="2013-07-24" version="1">MKLTAVIMAGGRGERFWPRSRTAKPKQFLSLTADKETMLQKTVNRLKPLLEMDAIYVVTNQRYAALVQEQLPELPAENILPEPCSRNTAPCIGLAAAVIQKRCGEAMMLVLPSDHLIRYESLYVDTLRQAIAVAEQGERLVTIGIPPTYPETGYGYIRFANRSESHMPGVYPVEKFVEKPDLDTAKEYLAAHCYLWNSGMFVWKSSTILHNLEQFLPTVYAGSMEIAAADGTDAFSDTLERVYEGFPSESVDYGIMEKADQIFTVPGSFGWDDVGNWLAMERINKTNDYGNYIEGDVITVNTQRSIICGQKKLIAAVGVENLIVIDTEDATLICAKDSTQDVKKVIENLRICNRDALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5V262</accession>
    <name>R5V262_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Rhomboid domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_00476</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000257">
        <property type="protein sequence ID" value="CCZ84623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00476"/>
    <dbReference type="EnsemblBacteria" id="CCZ84623">
        <property type="protein sequence ID" value="CCZ84623"/>
        <property type="gene ID" value="BN566_00476"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="SSF144091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="63"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="102"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="159"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="Rhomboid" evidence="6">
        <location>
            <begin position="60"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ84623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="216" mass="25528" checksum="30760379C67CBEB4" modified="2013-07-24" version="1">MQSTWLDKLERKFRKFSIPNLMNLILVGMAVVFLSDIFLSVQYGTTLSSYLYFSRSAIFHGEVWRILSFLFIPINSSPIWILFSMYFYWLIGSGLEREWGSFRFNVFYLCGAIGTILSGLITGYVGNTYLNFSLFLAFALLYPDFQVMLFFILPVKMKWLAWLDVAFLALEFLRSSWSGKLAILFAFANILLFFGKDFWQFIQNQIRRYQYRKKMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>R5V270</accession>
    <name>R5V270_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 10">Adenylosuccinate lyase</fullName>
            <shortName evidence="10">ASL</shortName>
            <ecNumber evidence="4 10">4.3.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 10">Adenylosuccinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BN566_00322</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + fumarate</text>
            <dbReference type="Rhea" id="RHEA:23920"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:58443"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="EC" id="4.3.2.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:23921"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N(6)-(1,2-dicarboxyethyl)-AMP = AMP + fumarate</text>
            <dbReference type="Rhea" id="RHEA:16853"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="4.3.2.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:16854"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.2" evidence="4 10"/>
    <dbReference type="EMBL" id="CBAR010000225">
        <property type="protein sequence ID" value="CCZ84415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00322"/>
    <dbReference type="EnsemblBacteria" id="CCZ84415">
        <property type="protein sequence ID" value="CCZ84415"/>
        <property type="gene ID" value="BN566_00322"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00132"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00075">
        <property type="reaction ID" value="UER00336"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070626">
        <property type="term" value="F:(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido) succinate lyase (fumarate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004018">
        <property type="term" value="F:N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019468">
        <property type="entry name" value="AdenyloSucc_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004769">
        <property type="entry name" value="Pur_lyase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10397">
        <property type="entry name" value="ADSL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00998">
        <property type="entry name" value="ADSL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="SSF48557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00928">
        <property type="entry name" value="purB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 10" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ADSL_C" evidence="11">
        <location>
            <begin position="371"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CCZ84415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="478" mass="54183" checksum="E10C0237CEF71F82" modified="2013-07-24" version="1">MRNTTSYESPFCTRYASEEMQHVFSADKKFSTWRKLWVALARAEMELGLPITPSQVKELEDNVYNIDYDVAEARERVVRHDVMSHVYAYGQVCPHAKGIIHLGATSCYVGDNTDIIIMRDALKIIRRKLINVLANLSKFAMQYKGMPALAYTHLQPAQLTTVGKRATLWMNELLMDLEELDFRLANLKLLGSKGTTGTQASFMELFEGDSTKVKKLEQMIAEEMGFTGVVPVSGQTYSRKIDSQILATLSGIAQSCSKFSNDMRLLQSFKEMEEPFEKTQIGSSAMAYKRNPMRCERITSLSRYVMIDSLNPAFTAGTQWFERTLDDSANKRISVPEACLGVDAILNIMLNVTDGLVVYPKMIRQRIMRELPFMATENIMMDAVKKGGDRQALHEKIREYSMIAGKHVKEDGLENDLCDMILADPMFMITKEELDAILQPENFTGRSEQQTEEFVDNCIRPILAANASLIGEHYEMKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5V295</accession>
    <name>R5V295_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00516</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 4-oxalocrotonate tautomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000267">
        <property type="protein sequence ID" value="CCZ84663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00516"/>
    <dbReference type="EnsemblBacteria" id="CCZ84663">
        <property type="protein sequence ID" value="CCZ84663"/>
        <property type="gene ID" value="BN566_00516"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.429.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001398">
        <property type="entry name" value="Macrophage_inhib_fac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014347">
        <property type="entry name" value="Tautomerase/MIF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11954">
        <property type="entry name" value="PTHR11954"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01187">
        <property type="entry name" value="MIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55331">
        <property type="entry name" value="SSF55331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="113" mass="12441" checksum="52EBBD6D8948E9BA" modified="2013-07-24" version="1">MPFMQLKTNVAVTEQQETLLKTSFGSAITAIPGKSETWLMVGIEPQYTLYFQGSDAPAAMVEVSIFGSASDAAYADLTARICKIVAETLHIDASRIYVKYAEIEHWGWNGSNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5V2U5</accession>
    <name>R5V2U5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00089</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000252">
        <property type="protein sequence ID" value="CCZ84595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84595">
        <property type="protein sequence ID" value="CCZ84595"/>
        <property type="gene ID" value="BN566_00089"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="88" mass="10235" checksum="D0724281DB8F2882" modified="2013-07-24" version="1">MHAALTALLEPKEQYQFPVYVGFSERYLSSLEQTYGFLAVTTHERLLVSRFSLLTIPMKQQAFALQTLQKLSVRQIPLLKTRKITMQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5V2W5</accession>
    <name>R5V2W5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00468</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000257">
        <property type="protein sequence ID" value="CCZ84615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00468"/>
    <dbReference type="EnsemblBacteria" id="CCZ84615">
        <property type="protein sequence ID" value="CCZ84615"/>
        <property type="gene ID" value="BN566_00468"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006807">
        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="SSF56317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CN hydrolase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="260" mass="29154" checksum="9DB21B8C2C7D7B6A" modified="2013-07-24" version="1">MRIALAQTEIFSKQYEKNKKTALSICNLAQAMEVRLLLFPEMSFTGFNMDTDYSIAAGLDTVPWMQKLATTGQCYLGFGWVKAAESGKAENHYSIVAPDGSLFFDMVKLHPFSYAQEDQFFEKGNALPTCEIDGIRFGCLICYDLRFPEVFSALAPKTDCMLVPANWPERRKLHWCSLLTARAIENQVYVAGINCVGTQDGLHYSGDSRFIAPDGTILQDCRSAAGCFVCDLSAKTVAETRQVFPVQKDRRPDFYRSILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5V2X0</accession>
    <name>R5V2X0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sortase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00473</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000257">
        <property type="protein sequence ID" value="CCZ84620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00473"/>
    <dbReference type="EnsemblBacteria" id="CCZ84620">
        <property type="protein sequence ID" value="CCZ84620"/>
        <property type="gene ID" value="BN566_00473"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05827">
        <property type="entry name" value="Sortase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005754">
        <property type="entry name" value="Sortase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042002">
        <property type="entry name" value="Sortase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023365">
        <property type="entry name" value="Sortase_dom-sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04203">
        <property type="entry name" value="Sortase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63817">
        <property type="entry name" value="SSF63817"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01076">
        <property type="entry name" value="sortase_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="249"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="296" mass="32926" checksum="F097641EFDBC458C" modified="2013-07-24" version="1">MRKKKTGVISTIFLILILLVGLSVMLYPTISDWWNSKVQTRAIASYSQAVEELDDGEAELLLQQAKDYNKDLYNLPAPFTNCDQLSAYDSLLNISGTGIMGYITIPVIHVELPIYHGTSEEVLNIAAGHLKGSSLPVGGANTHSVISAHRGLPSAKLFTDIDQLVVGDTFTITVLDQVLTYQVEQIRIILPEEIENLYIEPEQDMVTLMTCTPYGVNTHRLLIRSKRIDTKYQSHATVAADAVQLDPMLVVPCLSAPLLLGLFIFWGVSGKRNKRHFPHQDPLSLFSEEPPKKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>R5V2Y7</accession>
    <name>R5V2Y7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Thiamine-phosphate synthase</fullName>
            <shortName evidence="8">TP synthase</shortName>
            <shortName evidence="8">TPS</shortName>
            <ecNumber evidence="8">2.5.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Thiamine-phosphate pyrophosphorylase</fullName>
            <shortName evidence="8">TMP pyrophosphorylase</shortName>
            <shortName evidence="8">TMP-PPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">thiE</name>
        <name evidence="12" type="ORF">BN566_00493</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8 9">
            <text>2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2 H(+) = CO2 + diphosphate + thiamine phosphate</text>
            <dbReference type="Rhea" id="RHEA:47848"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
            <dbReference type="ChEBI" id="CHEBI:62890"/>
            <dbReference type="EC" id="2.5.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 8 9">
            <text>2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2 H(+) = CO2 + diphosphate + thiamine phosphate</text>
            <dbReference type="Rhea" id="RHEA:47844"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
            <dbReference type="ChEBI" id="CHEBI:62899"/>
            <dbReference type="EC" id="2.5.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 8 9">
            <text>4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 4-methyl-5-(2-phosphooxyethyl)-thiazole + H(+) = diphosphate + thiamine phosphate</text>
            <dbReference type="Rhea" id="RHEA:22328"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
            <dbReference type="ChEBI" id="CHEBI:58296"/>
            <dbReference type="EC" id="2.5.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 8 10">Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine phosphate from 4-amino-2-methyl-5-diphosphomethylpyrimidine and 4-methyl-5-(2-phosphoethyl)-thiazole: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8 9">Belongs to the thiamine-phosphate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.3" evidence="8"/>
    <dbReference type="EMBL" id="CBAR010000257">
        <property type="protein sequence ID" value="CCZ84640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00493"/>
    <dbReference type="EnsemblBacteria" id="CCZ84640">
        <property type="protein sequence ID" value="CCZ84640"/>
        <property type="gene ID" value="BN566_00493"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00060">
        <property type="reaction ID" value="UER00141"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004789">
        <property type="term" value="F:thiamine-phosphate diphosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00564">
        <property type="entry name" value="TMP_TenI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00097">
        <property type="entry name" value="TMP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036206">
        <property type="entry name" value="ThiamineP_synth_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022998">
        <property type="entry name" value="ThiamineP_synth_TenI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034291">
        <property type="entry name" value="TMP_synthase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02581">
        <property type="entry name" value="TMP-TENI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51391">
        <property type="entry name" value="SSF51391"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00693">
        <property type="entry name" value="thiE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8 9" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="5 8 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TMP-TENI" evidence="11">
        <location>
            <begin position="10"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="HMP-PP binding" evidence="8">
        <location>
            <begin position="40"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="THZ-P binding" evidence="8">
        <location>
            <begin position="137"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="THZ-P binding" evidence="8">
        <location>
            <begin position="187"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine</name>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine</name>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine</name>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:62899"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CCZ84640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="216" mass="22738" checksum="8C4D9195EFEA11AF" modified="2013-07-24" version="1">MQLTDAQMRLYAITDGRWLNGRTLYDCVEDALKGGVTMLQIREKHCSTEKLTALAIPIVSLCHRYHVPCIINDDVQAAKDSGADGVHVGQDDTSIAQARAVLGAEKWIGTSAHNVAEAKKAQQDGADYIGTGAVFGSATKQDATPLSLQTLQEICQAVTIPVVAIGGITAENALQLRGFGGQGIAVISAIFAKPDCMTAAKEMRQLADVICKGAGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5V3U7</accession>
    <name>R5V3U7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DAGKc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00579</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the diacylglycerol/lipid kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000007">
        <property type="protein sequence ID" value="CCZ82774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00579"/>
    <dbReference type="EnsemblBacteria" id="CCZ82774">
        <property type="protein sequence ID" value="CCZ82774"/>
        <property type="gene ID" value="BN566_00579"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005218">
        <property type="entry name" value="Diacylglycerol/lipid_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001206">
        <property type="entry name" value="Diacylglycerol_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045540">
        <property type="entry name" value="YegS/DAGK_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00781">
        <property type="entry name" value="DAGK_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19279">
        <property type="entry name" value="YegS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00046">
        <property type="entry name" value="DAGKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="SSF111331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00147">
        <property type="entry name" value="TIGR00147"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50146">
        <property type="entry name" value="DAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DAGKc" evidence="2">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ82774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="300" mass="33379" checksum="F0C826CD5ECC4B2E" modified="2013-07-24" version="1">MKRVYFIFNLHSGKAALNNQIPKLLDQFTAAGYEVTVHPTQRQGDGIVSAKYACEHGFDLLVCAGGDGTLNETIQGCMNSENPLPVGYIPAGSTNDFARGLQIPTTPEKAVQCILDGNTLCCDIGKFNEHYFTYVAAFGALTEISYQTPQNYKNVLGHAAYLLNGIAHLPTIKARKMRIEYDGKTLENNYLYGMVTNATSVAKLLSLSDVEWDDGLFEVTLIRKPTDLVQFHQLILSLANFQLGAERQYFDYFRASHVTITNLDEEEVAWTIDGEYGGNQRVNEISNCQKALNIFVPKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-09-29" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5V422</accession>
    <name>R5V422_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000013">
        <property type="protein sequence ID" value="CCZ82864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82864">
        <property type="protein sequence ID" value="CCZ82864"/>
        <property type="gene ID" value="BN566_00100"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="214"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="488" mass="56958" checksum="2DEAEA9E4EBC590E" modified="2013-07-24" version="1">MFQYDISFQDRKHTTKPTGKEIGIISNHLVETKMDYRKLVSEVGEAGCSFCPAVYHGKRRAENFKSQQIIGLDFDSGVPFHIIQERAKYYHLKMLFAYKTFSHTIEHERFRVVFALQHKITDSFTAKSIVSIFMKIFENCDEACKDSARLFFGGKGLLHLASKPREISLGEIILAFTAYMFDKYGSKHYTRELQKFYIANHIRFEKNHPITDHGSFIPETSPQPVRSHASDKLKNGRRQVTRNLDWNILYDRCRLYRRFFDGSEYYYYPELFHIATNLINIEKGKAQFLHILQSEQNARYEAYHQRNWKQILNMLMEMDYQPTGCQVCPYEKDCLHAKNMVLTAKPGDCTILPIKKQEYVSIEEAEWDLENKFLEAMHSKNRGLHIIKAQTGIGKTNLYLNYIKAHPNEKFLIAVPTHKLKTEIYNKALQLGIENICCTPELPYFSDELKGEIEHLYRIGAGKSVGDYLIKYSSTLICISTFRIKFDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5V4R3</accession>
    <name>R5V4R3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pseudouridine synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00877</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000053">
        <property type="protein sequence ID" value="CCZ83089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00877"/>
    <dbReference type="EnsemblBacteria" id="CCZ83089">
        <property type="protein sequence ID" value="CCZ83089"/>
        <property type="gene ID" value="BN566_00877"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140098">
        <property type="term" value="F:catalytic activity, acting on RNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034470">
        <property type="term" value="P:ncRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001522">
        <property type="term" value="P:pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02869">
        <property type="entry name" value="PseudoU_synth_RluA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PseudoU_synth_2" evidence="1">
        <location>
            <begin position="33"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="227" mass="24866" checksum="FC96338CBDAEACD3" modified="2013-07-24" version="1">MQFGDVIELRLPEVPSVVANAVRSVPICFETADFVVYHKPSDLPVHPSQNHYTDTLGNCFCAAYPGLACRPVNRLDRNTSGLCVFAKSAYAANQLQYHLQKRYYAVVEGVLTGSGTVSAPIAREQESIIIRCVRADGKPAVTHYRALQHNDKYTLLQLRLGTGRTHQIRVHMAYIGHPLAGDTLYGGRTDAIDRQALHCGRLILPLVQKTGTVTVGAALPEDMASLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5V4W6</accession>
    <name>R5V4W6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00941</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0047 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000067">
        <property type="protein sequence ID" value="CCZ83154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00941"/>
    <dbReference type="EnsemblBacteria" id="CCZ83154">
        <property type="protein sequence ID" value="CCZ83154"/>
        <property type="gene ID" value="BN566_00941"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001602">
        <property type="entry name" value="UPF0047"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035917">
        <property type="entry name" value="YjbQ-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30615">
        <property type="entry name" value="PTHR30615"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01894">
        <property type="entry name" value="UPF0047"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004681">
        <property type="entry name" value="UCP004681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111038">
        <property type="entry name" value="SSF111038"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00149">
        <property type="entry name" value="TIGR00149_YjbQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01314">
        <property type="entry name" value="UPF0047"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="141" mass="15315" checksum="5CF47671DCC772A0" modified="2013-07-24" version="1">MLYTYHLTTPVSGFVSITAQVKGAVAESGVQSGICVVYCPHTTAGITINENADPDVQRDLLLAYAEAFPDRKAFRHAEGNSAAHAKAAAMGSSATIIIEQGKLLLGTWQGIYFCEFDGVRNRTFYVKILADTAEKNRRESQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>R5V510</accession>
    <name>R5V510_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative ribose operon repressor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_00992</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000072">
        <property type="protein sequence ID" value="CCZ83209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00992"/>
    <dbReference type="EnsemblBacteria" id="CCZ83209">
        <property type="protein sequence ID" value="CCZ83209"/>
        <property type="gene ID" value="BN566_00992"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01392">
        <property type="entry name" value="HTH_LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000843">
        <property type="entry name" value="HTH_LacI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046335">
        <property type="entry name" value="LacI/GalR-like_sensor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00356">
        <property type="entry name" value="LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13377">
        <property type="entry name" value="Peripla_BP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00354">
        <property type="entry name" value="HTH_LACI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50932">
        <property type="entry name" value="HTH_LACI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="5">
        <location>
            <begin position="3"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="HTH lacI-type" evidence="4">
        <location>
            <begin position="6"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ83209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="341" mass="38790" checksum="73FD5131DF53CBD2" modified="2013-07-24" version="1">MAKKNVTFADIAAYTGFSKTTISRYFNNPDSITVENQEKIANALEILNYKENKLARVMANGKTEMVGVIIPNLYMHYYSDMLDHIISTYEKYGYKFIVFAGNEDAEVERQYIQELLAYKTEGLIILSYTLSSKELSEYHIPIVTIEREDEYVCSVNTDNYMGGMQAATILAKSGCDILIHANADFPSNIPAYGRIQGFQDFCKANGFEHRILIRDFGASFEENNVEIAKLLQEIEEAYSEKKVGIFMPNDTHANILLNCIIRKYGMLPERYKIVGFDNSPVSREAVIPITTVGQQIDKIAQEAVELLSQQINERKKRRPKPLSAPIHKVLTPVLIRRETTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5V514</accession>
    <name>R5V514_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Glycosyl hydrolase family 32</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_00997</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000073">
        <property type="protein sequence ID" value="CCZ83214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00997"/>
    <dbReference type="EnsemblBacteria" id="CCZ83214">
        <property type="protein sequence ID" value="CCZ83214"/>
        <property type="gene ID" value="BN566_00997"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.115.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001362">
        <property type="entry name" value="Glyco_hydro_32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013189">
        <property type="entry name" value="Glyco_hydro_32_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013148">
        <property type="entry name" value="Glyco_hydro_32_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08244">
        <property type="entry name" value="Glyco_hydro_32C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00251">
        <property type="entry name" value="Glyco_hydro_32N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00640">
        <property type="entry name" value="Glyco_32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="SSF49899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="SSF75005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyco_hydro_32N" evidence="5">
        <location>
            <begin position="6"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_hydro_32C" evidence="6">
        <location>
            <begin position="328"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ83214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="433" mass="49590" checksum="C215BA7F3F856A3C" modified="2013-07-24" version="1">MMQNIHFKAPNCWINDPNGFIWYKGQYHLFYQCFPYAPQWGRMHWGHAVSKDLVNWEEKGIALYPTKTDDRSGCFSGSAVEQDGTLYLFYTGVNYLEENPEDINQCQNDQFLAAQLMISSDDGMTFDNVKRKKTIIPPIAEEAIGNKTHTRDPKVWRGKDAWYLVLGSSVDHKGRLLIYKSSDLQNWTYLSHVEKDNFGWMWECPDYFEIDGKGITVFSPMEFLNDGKAYDSAAICMFSAFDESTGTMQLAEDYQFLDYGLDLYAPQSTTDEDGNRIVIAWMRMPQAVDGKWNGMMCIPRVVSVKNQQIYFQPHPHIRNAFVKEISSPKQAGKSGYLVKTTLENGECINIGGYQIWRKQNKLFTDRSAVFVKDDNYRLTAETPVLKNGCELEIYVDSNLIEVYANQGEAVISSVVYGLSNHIDGEAYTLYTTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5V523</accession>
    <name>R5V523_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01007</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000076">
        <property type="protein sequence ID" value="CCZ83224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01007"/>
    <dbReference type="EnsemblBacteria" id="CCZ83224">
        <property type="protein sequence ID" value="CCZ83224"/>
        <property type="gene ID" value="BN566_01007"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="230" mass="24791" checksum="71633243B2684236" modified="2013-07-24" version="1">MIYSNEVNKMCSVAQGVNHGAAPIPEEAKWVKAKEISDISGLTHGIGWCAPQQGTCKLTLNVKEGIIQEALVETVGCSGMTHSAAMAAEILPGRTILEALNTDLVCDAINTAMRELFLQIVYGRSQSAFSEEGLPIGAGLEDLGKGLRSQVGTMYGTLAKGPRYLEMTDGYVTKIALNEDDEIIGYKFVNFGKMMDFIKAGDDANTAFEKAQGQYGRVADAVKFVDPREE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5V533</accession>
    <name>R5V533_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein conserved in bacteria</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01015</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000080">
        <property type="protein sequence ID" value="CCZ83234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01015"/>
    <dbReference type="EnsemblBacteria" id="CCZ83234">
        <property type="protein sequence ID" value="CCZ83234"/>
        <property type="gene ID" value="BN566_01015"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05826">
        <property type="entry name" value="Sortase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005754">
        <property type="entry name" value="Sortase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023365">
        <property type="entry name" value="Sortase_dom-sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009835">
        <property type="entry name" value="SrtB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04203">
        <property type="entry name" value="Sortase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63817">
        <property type="entry name" value="SSF63817"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004394867" evidence="3">
        <location>
            <begin position="26"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="34"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="282"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="290"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="310" mass="35176" checksum="0DC2F2341C95FD6A" modified="2013-07-24" version="1">MKKRNRILVALLAAISVLTAGCSGEIEQNPIGLEQEESQAEASADASESTTENEKAPDMLASQKKLYNQNKDTIGWIEVPGTSVDNVIVQKKDEAENEKNTYYLDKDFSHQKFRAGTVFMDYRDVFGYKEDEFSENLVLYGHNMADNTMFGSLRRYRQDLEFYKDAPFIKLSSNYKEYEYVIFGLVITSGDADNGGDNYADPMTWTGFPYWNMEELDDKTHFDYYVNTIESLNMIDNVVDVKYGDQLLTLSTCYSDEDNSRFLIVARQLRDGETEASLKKLITGGVAETDAEESDTDSTTDTEEETSSEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5V5A2</accession>
    <name>R5V5A2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01090</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83309">
        <property type="protein sequence ID" value="CCZ83309"/>
        <property type="gene ID" value="BN566_01090"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="78" mass="8934" checksum="0B30299AA40097B1" modified="2013-07-24" version="1">MKHNECSLLQKAKQIPRIALVRKAPPATGEFAVFLRILAFSKEAQDAEWQALMLEIQEERQTLQENGLIPTEQENSEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5V5C3</accession>
    <name>R5V5C3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01115</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000089">
        <property type="protein sequence ID" value="CCZ83334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01115"/>
    <dbReference type="EnsemblBacteria" id="CCZ83334">
        <property type="protein sequence ID" value="CCZ83334"/>
        <property type="gene ID" value="BN566_01115"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="47" mass="5139" checksum="40FDBC52C5FA457E" modified="2013-07-24" version="1">MVLKKATVQVLLLLIGMAMLVFGIWRGEAQTVFSKAIRLCMECVGIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5V5G9</accession>
    <name>R5V5G9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01155</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000093">
        <property type="protein sequence ID" value="CCZ83379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83379">
        <property type="protein sequence ID" value="CCZ83379"/>
        <property type="gene ID" value="BN566_01155"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="149" mass="16971" checksum="8622090CD6D37012" modified="2013-07-24" version="1">MAFVNAYLTEEDKKFIASFKFHNPIGRLNELAPLPEEWSIDKENKYYLICLGGQGELDSEYPPNYYKLIVNIDVVDIKARYQTVGNGKIGIKITWKIESIVVNSKKTDVDIQQIAMLALKSYGNIHFGGHIICSEFMSIPVPHYLKGEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5V5J0</accession>
    <name>R5V5J0_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptide chain release factor 1</fullName>
            <shortName evidence="5">RF-1</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">prfA</name>
        <name evidence="7" type="ORF">BN566_01175</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 5">Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="5">Methylated by PrmC. Methylation increases the termination efficiency of RF1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the prokaryotic/mitochondrial release factor family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000095">
        <property type="protein sequence ID" value="CCZ83399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01175"/>
    <dbReference type="EnsemblBacteria" id="CCZ83399">
        <property type="protein sequence ID" value="CCZ83399"/>
        <property type="gene ID" value="BN566_01175"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016149">
        <property type="term" value="F:translation release factor activity, codon specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00093">
        <property type="entry name" value="Rel_fac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005139">
        <property type="entry name" value="PCRF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000352">
        <property type="entry name" value="Pep_chain_release_fac_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045853">
        <property type="entry name" value="Pep_chain_release_fac_I_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004373">
        <property type="entry name" value="RF-1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03462">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00472">
        <property type="entry name" value="RF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00937">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75620">
        <property type="entry name" value="SSF75620"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00019">
        <property type="entry name" value="prfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00745">
        <property type="entry name" value="RF_PROK_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0488">Methylation</keyword>
    <keyword evidence="4 5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RF_PROK_I" evidence="6">
        <location>
            <begin position="227"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="modified residue" description="N5-methylglutamine" evidence="5">
        <location>
            <position position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ83399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="363" mass="41469" checksum="ABDE3CB9F5944677" modified="2013-07-24" version="1">MLEKLSRMEKNYTELADSLSDPAVLADNRLYAEKMREYKRMTPIIETYHAYLHAMQQRDEAKELLQENDGDAELKELAQEEYHTAKAACEDLHEKLIFLLLPQDPNDDKNVIIEIRSGAGGEEAALFANSLYRMYTMYAESKGWKQEVLNQNPTELGGFKEISFSIVGEGAYSKLKYESGVHRVQRVPETESQGRIHTSTVTVAVLVEADDVELNINPTDLRIDVFRASGAGGQHINKTESAVRITHLPTGIVVECQDERSQFKNKDKAMKILRSRLYEAMQREQNEKIASERRMQVGTGDRSERIRTYNFPQGRMTDHRIGLTLYRLEDLLNGNLEEVFEALATSEQLKKLAAKTMEDTEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>R5V5K0</accession>
    <name>R5V5K0_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosome-binding factor A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rbfA</name>
        <name evidence="2" type="ORF">BN566_01185</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of several proteins that assist in the late maturation steps of the functional core of the 30S ribosomal subunit. Associates with free 30S ribosomal subunits (but not with 30S subunits that are part of 70S ribosomes or polysomes). Required for efficient processing of 16S rRNA. May interact with the 5'-terminal helix region of 16S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer. Binds 30S ribosomal subunits, but not 50S ribosomal subunits or 70S ribosomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RbfA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000097">
        <property type="protein sequence ID" value="CCZ83409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01185"/>
    <dbReference type="EnsemblBacteria" id="CCZ83409">
        <property type="protein sequence ID" value="CCZ83409"/>
        <property type="gene ID" value="BN566_01185"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00003">
        <property type="entry name" value="RbfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000238">
        <property type="entry name" value="RbfA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023799">
        <property type="entry name" value="RbfA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020053">
        <property type="entry name" value="Ribosome-bd_factorA_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33515">
        <property type="entry name" value="PTHR33515"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02033">
        <property type="entry name" value="RBFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89919">
        <property type="entry name" value="SSF89919"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00082">
        <property type="entry name" value="rbfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01319">
        <property type="entry name" value="RBFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0690">Ribosome biogenesis</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="118" mass="13496" checksum="D0B891232399A155" modified="2013-07-24" version="1">MANYKTSRTEEDILRELTALLRELKDPRIDPLLTIVRVELSNDMSNCKVYVSSLSGIETAKTSCIGLVSATGFLRRELFHRLKLRKSPALHFIADHSIEHSAEINQKLHDLHLEDIEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5V5L7</accession>
    <name>R5V5L7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01198</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 130 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000102">
        <property type="protein sequence ID" value="CCZ83424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01198"/>
    <dbReference type="EnsemblBacteria" id="CCZ83424">
        <property type="protein sequence ID" value="CCZ83424"/>
        <property type="gene ID" value="BN566_01198"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.115.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007184">
        <property type="entry name" value="Mannoside_phosphorylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34106">
        <property type="entry name" value="PTHR34106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04041">
        <property type="entry name" value="Glyco_hydro_130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016202">
        <property type="entry name" value="PH1107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="SSF75005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="339" mass="38281" checksum="BF4DF52959502771" modified="2013-07-24" version="1">MNPITLKTDVLNNLPWQARPTDCTAPLWRYQENPIIRRNPIPGVARIFNSAVLPYEGAYIGVFRGEQTNGIPFIYLGRSKDGIHWEFEPEKIAFVDEAGKPFMPKYAYDPRLVKVEDTYYIIWCQDAYGAAIGMAKTTDFKTFVRVENPFLPFNRNAVLFPRKVDGKFLMLSRPSDSGHTPFGDIFLSESKDLEYWGHHRHVMGKSGRWWENVKIGGGAAPIETSEGWLLFYHGVTNTCNGFVYSIGGALLDLEEPSKVLYRCSDFLLTPEMPYEESGFVPNVCFPCAALCDAETGRIAIYYGCADSYVGLAFTTAETVISYIKEHDAAEADDRELGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>R5V6N7</accession>
    <name>R5V6N7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Radical SAM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_01519</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the radical SAM superfamily. Anaerobic sulfatase-maturating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000161">
        <property type="protein sequence ID" value="CCZ83784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01519"/>
    <dbReference type="EnsemblBacteria" id="CCZ83784">
        <property type="protein sequence ID" value="CCZ83784"/>
        <property type="gene ID" value="BN566_01519"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023885">
        <property type="entry name" value="4Fe4S-binding_SPASM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000385">
        <property type="entry name" value="MoaA_NifB_PqqE_Fe-S-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024025">
        <property type="entry name" value="SCIFF_rSAM_maturase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023867">
        <property type="entry name" value="Sulphatase_maturase_rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43273">
        <property type="entry name" value="PTHR43273"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13186">
        <property type="entry name" value="SPASM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01384">
        <property type="entry name" value="thioether_bond_formation_requi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04085">
        <property type="entry name" value="rSAM_more_4Fe4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03974">
        <property type="entry name" value="rSAM_six_Cys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01305">
        <property type="entry name" value="MOAA_NIFB_PQQE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="91"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="463" mass="52916" checksum="AB47588F8991DE8A" modified="2013-07-24" version="1">MIHKYKLHGYNVVLDVNSGGVHLVDDLTYDMLDNVAPPFAEVCPPEVVEKLSAFYQEADILSCYDEVLEAYNEQILFSEDDYEKYADCSVASPIKAMCLHIAHDCNLRCEYCFASTGDFGEGRKLMPLETGKQAIDFLLKHSANRKNLELDFFGGEPLMNFEVVKQVVAYARSKEQEYGKHFRFTITTNGMLLNDDNIDFINKEMSNVVLSIDGRKAVNDRMRKRVDGKGSYDIILPKYQRLVAQRGDKEYYVRGTYTKYNLDFSNDVFDLLEHGFDQISVEPVMADENDPYAITEADLPAIFKEYEVLMNKILDYEKTGKKFNFFHFMLDLDQGPCAIKRLRGCGCGNEYVAITPDGDIYPCHQFVGMEEWKMGNLEEGTFDQAKKADFAKAHIYTKPDCRECWARFYCSGGCNANNYQYAGDIRKAHKLSCEIEKKRLECAIVMKAIRMEQAEQAAAESNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5V6U9</accession>
    <name>R5V6U9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Bacillus/Clostridium GerA spore germination protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01569</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GerABKA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000166">
        <property type="protein sequence ID" value="CCZ83834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01569"/>
    <dbReference type="EnsemblBacteria" id="CCZ83834">
        <property type="protein sequence ID" value="CCZ83834"/>
        <property type="gene ID" value="BN566_01569"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009847">
        <property type="term" value="P:spore germination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004995">
        <property type="entry name" value="Spore_Ger"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03323">
        <property type="entry name" value="GerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005690">
        <property type="entry name" value="GerBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="290"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="357"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="383"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="413"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="484" mass="53485" checksum="578D69410EDA0645" modified="2013-07-24" version="1">MNEYAKERILPKLSQNLQRIREIAHNSSDLLVNEFVLSGLPAALLCCEGMLSTSTITELILRPIVEIQLEHPTAEQIFQHIQKHQLFSTDRAEALNYRDLFRFLNSGFAVLVLDGCPKALVFGVQGYDKRSVSTPSGENNIFGAKDGFVEVVRTNMSLIRRRMKSPFLKMELGCYGDKSQTDLCICYLYDRVPQALIQKIKRQLGSMKLESILSSGYVRPFLETSRPSLFDTISTTQRPDVFCAKLLEGRVGILIDGTPFALMIPKLFNESFQSMDDYCYKPYYATFLRWLKYAAFLVALLLPALYVAIALHHPELLNRTLLLILAESEETAPLSLTTEAIGILLVYEIIREAGLRLPQAVGGAVSIVAGLIVGDAAVSSGLISTPMLTVTAISVIAGFVIPDLNPPISILRLAFVLAGGMGGLFGISLLGAAVLFHICAAQNHGFPLTAPVAPFRKRGMRDILYRADFPAMQSGNFTVEEYHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5V735</accession>
    <name>R5V735_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Cell division septal protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN566_01638</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000172">
        <property type="protein sequence ID" value="CCZ83909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01638"/>
    <dbReference type="EnsemblBacteria" id="CCZ83909">
        <property type="protein sequence ID" value="CCZ83909"/>
        <property type="gene ID" value="BN566_01638"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034746">
        <property type="entry name" value="POTRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013685">
        <property type="entry name" value="POTRA_FtsQ_type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08478">
        <property type="entry name" value="POTRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51779">
        <property type="entry name" value="POTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 10" id="KW-0132">Cell division</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="27"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="POTRA" evidence="9">
        <location>
            <begin position="51"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="276"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ83909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="315" mass="35162" checksum="FC34A3050CB703DB" modified="2013-07-24" version="1">MPMQDVKKTDMKSETNVKRVRRRNRWLPLYIAVVLVLTAGVGVALSTTVFFNVENIVVTGEAEQYTVTDIVKAADVCSGDNLIKLNTDEVAKRVYDNLIYIETVDVQKKFPDELVINVQKCRESYNIVYDNGILLASATGKIISNSMEAAEGLPIFYGYLPSILSPGEKLKSQDEQKDKIFYLFAKIMSRELSSPITSVDMTDKYNMRVCFDDRIIFDLGNWNELEYKITLAENVIDRLGIDKSGYLTMVGNNQCSFRDSSVVGELDPIKPVETQVTTTTTETGTTNANGANSGSTETTTTVVTTTVPEEEQQYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>R5V8B9</accession>
    <name>R5V8B9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TetR family HTH transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00276</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000221">
        <property type="protein sequence ID" value="CCZ84369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84369">
        <property type="protein sequence ID" value="CCZ84369"/>
        <property type="gene ID" value="BN566_00276"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039532">
        <property type="entry name" value="TetR_C_Firmicutes"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14278">
        <property type="entry name" value="TetR_C_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="10"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="192" mass="22677" checksum="8674487A125B52B1" modified="2013-07-24" version="1">MKKSNDRRIRYTRYALQNALIVCMQQKPFNRITVREICETADINRSTFYMHYKDIYELLDEIEEQVYQEIDAIFSKESPSIHNMEQLLDYVQTHKTLLECLLQQGSGLAISRFSTILHAHYGKYLRRYAGNGFSAENEYLYEFLYTGILGVLQKWLQNECKDDRNQVASVMWRMIVQSLPHTKNPHSFSSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5V8K0</accession>
    <name>R5V8K0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00346</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000228">
        <property type="protein sequence ID" value="CCZ84439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84439">
        <property type="protein sequence ID" value="CCZ84439"/>
        <property type="gene ID" value="BN566_00346"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="99" mass="11168" checksum="F270C1CE257F10CE" modified="2013-07-24" version="1">MEDPTQPMFPVSEPESISAPQEPVIVTDPTPHTKKKKHNGDCMGMQLLLTGILAAAVFSLRWVNVSYQTALLQLYQTHFQAPAEPLLAQLLERLEFWVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>R5V8Y2</accession>
    <name>R5V8Y2_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B</fullName>
            <shortName evidence="10">Asp/Glu-ADT subunit B</shortName>
            <ecNumber evidence="10">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">gatB</name>
        <name evidence="12" type="ORF">BN566_00410</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>ATP + H2O + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + 2 H(+) + L-asparaginyl-tRNA(Asn) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14513"/>
            <dbReference type="Rhea" id="RHEA-COMP:9674"/>
            <dbReference type="Rhea" id="RHEA-COMP:9677"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78515"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 10">
            <text>ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) + L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4 10">Heterotrimer of A, B and C subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the GatB/GatE family. GatB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="10"/>
    <dbReference type="EMBL" id="CBAR010000243">
        <property type="protein sequence ID" value="CCZ84549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00410"/>
    <dbReference type="EnsemblBacteria" id="CCZ84549">
        <property type="protein sequence ID" value="CCZ84549"/>
        <property type="gene ID" value="BN566_00410"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050566">
        <property type="term" value="F:asparaginyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00121">
        <property type="entry name" value="GatB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004413">
        <property type="entry name" value="Apn/Gln-ADT_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017959">
        <property type="entry name" value="Asn/Gln-tRNA_amidoTrfase_suB/E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006075">
        <property type="entry name" value="Asn/Gln-tRNA_Trfase_suB/E_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018027">
        <property type="entry name" value="Asn/Gln_amidotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003789">
        <property type="entry name" value="Asn/Gln_tRNA_amidoTrase-B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042114">
        <property type="entry name" value="GatB_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023168">
        <property type="entry name" value="GatB_Yqey_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017958">
        <property type="entry name" value="Gln-tRNA_amidoTrfase_suB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659">
        <property type="entry name" value="PTHR11659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02934">
        <property type="entry name" value="GatB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02637">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00845">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="SSF55931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89095">
        <property type="entry name" value="SSF89095"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00133">
        <property type="entry name" value="gatB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01234">
        <property type="entry name" value="GATB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GatB_Yqey" evidence="11">
        <location>
            <begin position="328"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CCZ84549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="478" mass="52907" checksum="58CF19581D55EC7F" modified="2013-07-24" version="1">MQDYEMVVGLEVHAELNTASKIYCNCRNAFGLEVNTQVCPVCMGMPGTLPTLNEKVVEYAIKMGHAMNCTINAVSKQDRKNYFYPDLPKAYQISQAEVPLCENGYLDIMVDGEEKRIGVTRIHIEEDAGKLLHDASFSGSLVDLNRCGVPLIEIVSEPDIRSSAEAKAYLETIKSILQYLNISDCKMQEGSIRCDVNVSVRKKGSTEFGTRCEMKNVNSFSAAVRGIEYEAKRQIEVLEAGGTIAQETRRWDDAKGMNYTMRSKEDAQDYRYFPEPDLLSIVVPEEKIAALKASIPELPNAKIRRYVKDFGLSQVDATLIAEDLERSAMFDACIAIGTCKPKSISNWILSDIAKFTNDTGKKISETPLTPQRLTELIVQVEKGVISNSSAKKVFQIVLETGKEPLAIIEEKGMAQNSDTGFLEQLAKDVLANNEKSVNDYKNGKTNALGYLVGQCMKASKGKANPGIVKEIVTKMLSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>R5V985</accession>
    <name>R5V985_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ribosome maturation factor RimM</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rimM</name>
        <name evidence="6" type="ORF">BN566_00527</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">An accessory protein needed during the final step in the assembly of 30S ribosomal subunit, possibly for assembly of the head region. Probably interacts with S19. Essential for efficient processing of 16S rRNA. May be needed both before and after RbfA during the maturation of 16S rRNA. It has affinity for free ribosomal 30S subunits but not for 70S ribosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Binds ribosomal protein S19.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="3">The PRC barrel domain binds ribosomal protein S19.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RimM family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000267">
        <property type="protein sequence ID" value="CCZ84674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00527"/>
    <dbReference type="EnsemblBacteria" id="CCZ84674">
        <property type="protein sequence ID" value="CCZ84674"/>
        <property type="gene ID" value="BN566_00527"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00014">
        <property type="entry name" value="Ribosome_mat_RimM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011961">
        <property type="entry name" value="16S_RimM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027275">
        <property type="entry name" value="PRC-brl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002676">
        <property type="entry name" value="RimM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036976">
        <property type="entry name" value="RimM_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05239">
        <property type="entry name" value="PRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01782">
        <property type="entry name" value="RimM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="SSF50346"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02273">
        <property type="entry name" value="16S_RimM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="1 3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="RimM" evidence="4">
        <location>
            <begin position="6"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="PRC" evidence="5">
        <location>
            <begin position="92"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ84674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="169" mass="19013" checksum="B0B85C0876AD9E68" modified="2013-07-24" version="1">MKSYLEIGKIVTLHGLRGDVKVQPWCDDAEFLCEFPVLYLGKEKKEVEVSHARPQKNMVLLHLEGYDTPEQAETLRNQILYLNREDVELEEGTYFIQDLIGLDVIDADNGTAYGTLKDVLQTGANDVYVVQAEGGKELLIPAIPDVIVETNIEEHCMKIRPLEGLLDAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>R5VGX7</accession>
    <name>R5VGX7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">MiaB-like tRNA modifying enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN566_00631</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000011">
        <property type="protein sequence ID" value="CCZ82826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00631"/>
    <dbReference type="EnsemblBacteria" id="CCZ82826">
        <property type="protein sequence ID" value="CCZ82826"/>
        <property type="gene ID" value="BN566_00631"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035596">
        <property type="term" value="F:methylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005839">
        <property type="entry name" value="Methylthiotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020612">
        <property type="entry name" value="Methylthiotransferase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013848">
        <property type="entry name" value="Methylthiotransferase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038135">
        <property type="entry name" value="Methylthiotransferase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006467">
        <property type="entry name" value="MiaB-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00919">
        <property type="entry name" value="UPF0004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01061">
        <property type="entry name" value="methylthiotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01579">
        <property type="entry name" value="MiaB-like-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00089">
        <property type="entry name" value="TIGR00089"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51449">
        <property type="entry name" value="MTTASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01278">
        <property type="entry name" value="MTTASE_RADICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MTTase N-terminal" evidence="9">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="10">
        <location>
            <begin position="139"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ82826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="444" mass="49026" checksum="47C00FD762021DC6" modified="2013-07-24" version="1">MNCYFFTFGCKVNSCETAGMEAIFQAAGYTITTQPEQADVIVLNTCTVTASGDSRMHTALRRLRAGNPDAVMVLTGCFAQAFPEEAAALPEADLVIGTQDRDRLPQLVQQFLMGNRAPMQHIPAYTGTEAFACLPHHLPADRTRAFLKIQDGCNCFCSYCIIPYARGRCRSMPLDVLQQEVSQFAAEGYTEIVLCGINLAFYGKEWGGSLLDAVSLCCQTSGIQRVRLGSLEPERMTADLLDALAALPAFCPQFHLSLQSGCDHTLKEMHRRYTTQEYAALCAAIRSRFPVCAITTDIMVGFPGETDADFAESLAFAESMQFAKIHVFRYSPRTGTPAAKRTDQISDSVKHTRMVAMQQLAETARTAFLSSRIGLTLPVLFERERNAAFHNGHTPDFTPVKIPAEKEKKSLRKSIFYVRIEESDSACCFGHIVPKDNANSSQKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>R5VH77</accession>
    <name>R5VH77_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RNA polymerase sigma factor SigA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">sigA</name>
        <name evidence="9" type="ORF">BN566_00708</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts transiently with the RNA polymerase catalytic core.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the sigma-70 factor family. RpoD/SigA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000022">
        <property type="protein sequence ID" value="CCZ82906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00708"/>
    <dbReference type="EnsemblBacteria" id="CCZ82906">
        <property type="protein sequence ID" value="CCZ82906"/>
        <property type="gene ID" value="BN566_00708"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00963">
        <property type="entry name" value="Sigma70_RpoD_SigA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000943">
        <property type="entry name" value="RNA_pol_sigma70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009042">
        <property type="entry name" value="RNA_pol_sigma70_r1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007624">
        <property type="entry name" value="RNA_pol_sigma70_r3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007630">
        <property type="entry name" value="RNA_pol_sigma70_r4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007127">
        <property type="entry name" value="RNA_pol_sigma_70_r1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042189">
        <property type="entry name" value="RNA_pol_sigma_70_r1_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012760">
        <property type="entry name" value="RNA_pol_sigma_RpoD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028630">
        <property type="entry name" value="Sigma70_RpoD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03979">
        <property type="entry name" value="Sigma70_r1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00140">
        <property type="entry name" value="Sigma70_r1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04539">
        <property type="entry name" value="Sigma70_r3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04545">
        <property type="entry name" value="Sigma70_r4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00046">
        <property type="entry name" value="SIGMA70FCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02393">
        <property type="entry name" value="RpoD_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00715">
        <property type="entry name" value="SIGMA70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00716">
        <property type="entry name" value="SIGMA70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="5 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RNA_pol_sigma70" evidence="7">
        <location>
            <begin position="149"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="RNA_pol_sigma70" evidence="8">
        <location>
            <begin position="318"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="6">
        <location>
            <begin position="319"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Sigma-70 factor domain-2" evidence="6">
        <location>
            <begin position="125"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Sigma-70 factor domain-3" evidence="6">
        <location>
            <begin position="204"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Sigma-70 factor domain-4" evidence="6">
        <location>
            <begin position="293"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Interaction with polymerase core subunit RpoC" evidence="6">
        <location>
            <begin position="149"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ82906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="358" mass="40214" checksum="7491E0BBA1C58CDC" modified="2013-07-24" version="1">MEGKTTIEKLMERGKATGELTTKEISDALEELDFDVDQLNSFYDTCDRLNIKIIDDTAPEISGKGGDADLVDDLEMALSTEGIAIDDPVKVYLKEIGRVPLLSSDEETELAKRMAEGDTKAKNRLSEANLRLVVSIAKKYVGRGMQFLDLIQEGNLGLLKAVEKFDYTKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETISKVKKVSSQLLHETGHDPSAEEIAEKLNMPAERVREIMRIAQDPVSLETPIGEEEDSHLGDFIPDDDAPAPADAASLMLLKEQLNDVLSTLTDREAKVLKLRFGLEDGRSRTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKVKDFLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5VHA2</accession>
    <name>R5VHA2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Pyridoxamine 5'-phosphate oxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00726</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000029">
        <property type="protein sequence ID" value="CCZ82926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00726"/>
    <dbReference type="EnsemblBacteria" id="CCZ82926">
        <property type="protein sequence ID" value="CCZ82926"/>
        <property type="gene ID" value="BN566_00726"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ82926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="90" mass="9500" checksum="D2606EF3EF40902B" modified="2013-07-24" version="1">MLTEAVKKVLAENLQANGGKIAISAYNATSLEGYQIKGTAEYVTSGELVETFKKAVETMFHGAATAKGALIVTPEQVIVTTPEAENKTVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VHE8</accession>
    <name>R5VHE8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00761</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000032">
        <property type="protein sequence ID" value="CCZ82961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82961">
        <property type="protein sequence ID" value="CCZ82961"/>
        <property type="gene ID" value="BN566_00761"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ82961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="79" mass="8803" checksum="67B962A5944B453A" modified="2013-07-24" version="1">MPVRKNDFTNHPVLLNDTAEHIIQIAKTCASIKELFAKSCMDYQIEEQSENGAAVMDFIQQLVSTKILIETNESCIGCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5VHN6</accession>
    <name>R5VHN6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00804</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000038">
        <property type="protein sequence ID" value="CCZ83011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83011">
        <property type="protein sequence ID" value="CCZ83011"/>
        <property type="gene ID" value="BN566_00804"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008965">
        <property type="entry name" value="CBM2/CBM3_carb-bd_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49384">
        <property type="entry name" value="SSF49384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004395106" evidence="1">
        <location>
            <begin position="29"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="308" mass="34526" checksum="F4E4C02A0484CF77" modified="2013-07-24" version="1">MKRFHKMVAAVMAVATLATSMVPLSADALYFNGQSKFAYDLSVDDANWDDMVRVTFYTTYNPGVTSFSVALKYDTSKLEYVESYRDSDYESAGTFGTSNDKTKGMVIIVSATENLQDYNQPISYSYYFKPKTTSRKETYNFSACVYQYNSRTENILFGNNIGKLSPVPDEVTKKVQKGTYNRRIGDVIPDNSIDMNDVTTMLGIVSFLNDCQESDVTDTYDLNEMLITDRAYTLKSGKTGTYRERLKDSLYVDDVPFAEVADCDQNGKIEKADADLLITYYAQAASGQDPDSLINSKISKVVYADITT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5VHP7</accession>
    <name>R5VHP7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DnaD and phage-associated domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00814</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000039">
        <property type="protein sequence ID" value="CCZ83021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00814"/>
    <dbReference type="EnsemblBacteria" id="CCZ83021">
        <property type="protein sequence ID" value="CCZ83021"/>
        <property type="gene ID" value="BN566_00814"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.630">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017019">
        <property type="entry name" value="DNA_replication_prd_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034829">
        <property type="entry name" value="DnaD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006343">
        <property type="entry name" value="DnaD_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07261">
        <property type="entry name" value="DnaB_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF033722">
        <property type="entry name" value="DnaD_CA_C3587_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158499">
        <property type="entry name" value="SSF158499"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01446">
        <property type="entry name" value="DnaD_dom"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DnaB_2" evidence="2">
        <location>
            <begin position="121"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="DnaB_2" evidence="2">
        <location>
            <begin position="213"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="275"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="319" mass="37073" checksum="4CE11E0A9CCD27F6" modified="2013-07-24" version="1">MTLILQNPAWQSLFSVPTAVVDQYLKLATPSQLKVLLYLLRHQPETIDTVQVGKQIGCLPEQVEEALLFWEQTDLFTPPEPPAPEKPQEVAALIRTSSELSILPTVIADAVQSSESLQTLFRMVQQLYGRPLNFMEEQSLVWLHDYLNFSSDIILMVVSYCHSIQKCNVRYIEQILIRWWNQGITTLQQAQEEIKDMEQRHSYLAQIMRLLEMRRNPTPKQMEFIDDWKQKAIPMDLIRYAYEKTVEKTDRLSFPYMQSILNDWMKAGYKTRAEVDLNDKPPESPRIRKKKQPEIPVSENAEAYASLIYNLDEPIGGNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5VHW2</accession>
    <name>R5VHW2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Resolvase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00903</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000059">
        <property type="protein sequence ID" value="CCZ83116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00903"/>
    <dbReference type="EnsemblBacteria" id="CCZ83116">
        <property type="protein sequence ID" value="CCZ83116"/>
        <property type="gene ID" value="BN566_00903"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038109">
        <property type="entry name" value="DNA_bind_recomb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011109">
        <property type="entry name" value="DNA_bind_recombinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025827">
        <property type="entry name" value="Zn_ribbon_recom_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07508">
        <property type="entry name" value="Recombinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13408">
        <property type="entry name" value="Zn_ribbon_recom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00857">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="SSF53041"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51737">
        <property type="entry name" value="RECOMBINASE_DNA_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="1">
        <location>
            <begin position="21"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Recombinase" evidence="2">
        <location>
            <begin position="175"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="432" mass="49731" checksum="590AFCB4124288F4" modified="2013-07-24" version="1">MKLERKVHRVQQRPPKPKLLRVVAYARVSSGKDAMLHSLAAQVDYFQNLIRQHPGWEFCGVFADEAKTGTKDERPEYQKMLEKCRNREVDLVITKSISRFARNTVTLLETVRELKSLGIDVYFEKENLHSTSGDGELMLSILASFAQEESKSVSDNMKWRIRNDFQQGKIGSITIFGYRRNADGVLEIEESEAKIVQMIFADYCSGMGQCAIAKKINAMGISTRQGNRWTGQRVKEILVNEKYVGNMLLQKYYRQDPIGKRKMKNQGELPKYFVEQSHESIISANLFKKVQNLVKERTEQFSHPGATNRYPLSGMVQCAGCGKSYQRKIYKQGAVWMCATYLRQGKSHCPTAKQISERILHEKICSVLQIKEFDVEIVKSRVDNILISPNQLTFLFKDNTEKTVSWENHSRSESWTTEMRQAAAERSRKCQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>R5VHX4</accession>
    <name>R5VHX4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">UvrABC system protein A</fullName>
            <shortName evidence="13">UvrA protein</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Excinuclease ABC subunit A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">uvrA</name>
        <name evidence="16" type="ORF">BN566_00923</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. When the presence of a lesion has been verified by UvrB, the UvrA molecules dissociate.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Forms a heterotetramer with UvrB during the search for lesions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the ABC transporter superfamily. UvrA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000062">
        <property type="protein sequence ID" value="CCZ83136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00923"/>
    <dbReference type="EnsemblBacteria" id="CCZ83136">
        <property type="protein sequence ID" value="CCZ83136"/>
        <property type="gene ID" value="BN566_00923"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009380">
        <property type="term" value="C:excinuclease repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009381">
        <property type="term" value="F:excinuclease ABC activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00205">
        <property type="entry name" value="UvrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004602">
        <property type="entry name" value="UvrA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041552">
        <property type="entry name" value="UvrA_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041102">
        <property type="entry name" value="UvrA_inter"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17755">
        <property type="entry name" value="UvrA_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17760">
        <property type="entry name" value="UvrA_inter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00630">
        <property type="entry name" value="uvra"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 13" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6 13" id="KW-0228">DNA excision</keyword>
    <keyword evidence="12 13" id="KW-0234">DNA repair</keyword>
    <keyword evidence="11 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10 13" id="KW-0267">Excision nuclease</keyword>
    <keyword evidence="2 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0742">SOS response</keyword>
    <keyword evidence="8 13" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ABC transporter" evidence="15">
        <location>
            <begin position="606"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="13">
        <location>
            <begin position="251"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="13">
        <location>
            <begin position="738"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="32"/>
            <end position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 14">
        <location>
            <begin position="639"/>
            <end position="646"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CCZ83136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="938" mass="103499" checksum="261C03D1BE49346C" modified="2013-07-24" version="1">MIDRIRVRGARANNLKNIDVDLPRDALVVMTGLSGSGKSSLAFDTIFADGQRRYMESLSSYARQFLGQMEKPDVDSIEGLSPAISIDQKTTSKNPRSTVGTVTEIYDYLRLLYARIGIPHCPICGREIRQQSIDEMVDRVLSLEEGTRIQLLAPIIRGKKGQHTKELEKARRSGYVRVKIDDSLYDLSETITLDKNKKHTIEIVVDRLVVKEGIRSRLTDSLETVFSLTGSLAAVEIMGKETWLFSQKYACPEHNISIEELTPRMFSFNNPYGACENCTGLGVFQKVDPDLIVPNVDLSIAQGAIKAPGWNSLDDNSIAMMYYQAVAESEHVSLDTPVKDLPDHAMKSFLYGTTKELFLHRIQENGRSTGYRAAFEGVIPNLERRAREGSEWAKAEISAYMSAVPCPVCHGKRLKKEVLGVTVGGLNIMELTDLSITESLQFFETLSLTEQEQKIAMQILKEIKERLGFLKNVGLEYLTLSRTAGTLSGGESQRIRLATQIGSYLMGVLYILDEPSIGLHQRDNDKLIATLKRLRDLGNTLIVVEHDDDTILAADYVVDIGPGAGVNGGNVVYSGDVAGLLACEDSITGQYLSGKKQIAVPEYRRVGNGNFLTVIGAAEHNLKNITVSIPLGTFTCVTGVSGSGKSSLVNEIIYKHLAAKLNRTRVRAGAFERMEGLEYLDKVINIDQSPIGRTPRSNPATYTGVFTDIRELFAKTNDAKTKGYTAGRFSFNIKGGRCEACQGDGILKIEMHFLPDVYVPCEVCKGKRYNRETLEVKYRGKSIYDVLEMTVDEGVVFFEQHPKIFRKLKMLQEVGLGYIKIGQPATTLSGGEAQRVKLSTELAKRPTGRTIYILDEPTTGLHTADVHRLIDVLQRLTDAGNTVLVIEHNLNVIKVADYLIDLGKEGGTAGGTLVVSGTPEEVVACADSYTGNYLKAYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VII4</accession>
    <name>R5VII4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01142</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000093">
        <property type="protein sequence ID" value="CCZ83366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83366">
        <property type="protein sequence ID" value="CCZ83366"/>
        <property type="gene ID" value="BN566_01142"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="107" mass="12718" checksum="6E8FED755CC6CB71" modified="2013-07-24" version="1">MAIDNAEVIDATAYEGKKLILQLYDHLEFDDEIIKEHMFMLQDKLNTYIWFIDSKQYEETYPNASLSVYEIQIKFKYQPSDFCIEYLNHVNNKLGSMNITVMFEVTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5VIN5</accession>
    <name>R5VIN5_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">YlxR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01182</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000097">
        <property type="protein sequence ID" value="CCZ83406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01182"/>
    <dbReference type="EnsemblBacteria" id="CCZ83406">
        <property type="protein sequence ID" value="CCZ83406"/>
        <property type="gene ID" value="BN566_01182"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00279">
        <property type="entry name" value="YlxR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007393">
        <property type="entry name" value="DUF448"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037465">
        <property type="entry name" value="YlxR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035931">
        <property type="entry name" value="YlxR-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34215">
        <property type="entry name" value="PTHR34215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04296">
        <property type="entry name" value="YlxR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64376">
        <property type="entry name" value="SSF64376"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YlxR" evidence="1">
        <location>
            <begin position="9"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="100" mass="11549" checksum="11F11AFCF2A2F976" modified="2013-07-24" version="1">MKKKKEVLRACMGCGERKPKQELIRIVRTPDGNFVIDETGKTPGRGAYLCKNKACLEKAKKTRKVERSFVQMFPPETYKEMEDALTRKVQELTDDLPESR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5VIQ2</accession>
    <name>R5VIQ2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">M6 family metalloprotease domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01192</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000099">
        <property type="protein sequence ID" value="CCZ83416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01192"/>
    <dbReference type="EnsemblBacteria" id="CCZ83416">
        <property type="protein sequence ID" value="CCZ83416"/>
        <property type="gene ID" value="BN566_01192"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004407139" evidence="1">
        <location>
            <begin position="34"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="2">
        <location>
            <begin position="745"/>
            <end position="812"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="812" mass="89078" checksum="0C24EF73938786A8" modified="2013-07-24" version="1">MKERVSLRRMTAFFTSILLVVFCLPTLALPAKAETTVRQVNNIVLFAQFDPLTEKNFMSGDATNTVLSMCNDTTTYRSLTKYIDTISYGQMHVDSYFPQLKDGVIEPYVLSQSRESYTDYNQYAVEMIQNIAIPADIPLDGDNDGYIDNIVCVVDGKVTSAADPLWSKAFYLDGGLQVNGLTVGQVNLHSGYSVIGSTLFNGIGTVCHEFLHSMGYPDLYHRSDVPGDPVGQWDIMATTSIFLQYPLAYQRYSVSGWMGAETITTAGTYALAPASASEGSRLYLLKTPLSDTEFFAVEYRKQGAKYSDELDGKIYGDGMVVYRVNTSVVGNYRGDTDQIYIFRPGETALNGGAGDLTKSCYGGTGAPNHIGTTDLQKKFSDGALVYADGTNSGIALDNIQIQDNGTLTFSVSFADLSGQKLWETSNNSSFSADTMAYDLATAADGTIYLLSTTAYSATLYRVEEDETLTAIRKPFSGSMYNPKLVICDGTAYVLYQDYDYFSHLCKWNENGQLWEECYVGTKLAQYQDLATDGEQLYFTCTTGSFPYALQAYCYNPNTGQATQIGTDLSGNACNMEIAAADGTVVIGYRDLTANSVPKAAIYNGATWNIITLSEQECGMVSVIAEEDGKFWVLPSGGKNPIFSVSKDGTATAYELPAALQENVFQMVPVLSGGNLYVAVNSQNPNAFDLYRLNQETASWETVGNTIANEIVNQPVLSARNGTIYCMYNASEQILLKKLVISKNEETILKGDLNLDGRRNLADTVLFHRYLLRTVTLTEEQGKRAETVEDGTINIIDLIWLKQAIHLVDVVDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJ50</accession>
    <name>R5VJ50_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH marR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01280</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000119">
        <property type="protein sequence ID" value="CCZ83511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01280"/>
    <dbReference type="EnsemblBacteria" id="CCZ83511">
        <property type="protein sequence ID" value="CCZ83511"/>
        <property type="gene ID" value="BN566_01280"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023187">
        <property type="entry name" value="Tscrpt_reg_MarR-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12802">
        <property type="entry name" value="MarR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00598">
        <property type="entry name" value="HTHMARR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01117">
        <property type="entry name" value="HTH_MARR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="159" mass="18090" checksum="0847B5BEC677569A" modified="2013-07-24" version="1">MMMHPEHHAFFEMMQQFRSLNLLATLPLPRQDYMVLYAISCLQKEHPDSCTVSMIAKKMQIPQPAVSRTLRSLENGGYLQREVCRSDRRNTYVSLTDAGKAAVQEADQTLATFHEGIQKRFTKEEAEQLMALLQRLYAAAAEQLAEMKEGAQKPDGKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJY6</accession>
    <name>R5VJY6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01591</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000167">
        <property type="protein sequence ID" value="CCZ83856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83856">
        <property type="protein sequence ID" value="CCZ83856"/>
        <property type="gene ID" value="BN566_01591"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="123" mass="13418" checksum="5421D23DE2DEBDAB" modified="2013-07-24" version="1">MDGMMEKMQSLLSDPESMQQLQELAQMLQADSGQESTETESAPTATEQTDATASGGGLDLGALMKVSQLMGSAKEDSDAALLLALKPHLREERQKKVEKAVKMLKLLSIWSMLKESGMLKDFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>R5VK66</accession>
    <name>R5VK66_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cation_ATPase_N domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN566_01655</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000173">
        <property type="protein sequence ID" value="CCZ83926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01655"/>
    <dbReference type="EnsemblBacteria" id="CCZ83926">
        <property type="protein sequence ID" value="CCZ83926"/>
        <property type="gene ID" value="BN566_01655"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="SSF81653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="SSF81660"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="SSF81665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-1278">Translocase</keyword>
    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="61"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="83"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="246"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="277"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="665"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="693"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="733"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="764"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="804"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="837"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="domain" description="Cation_ATPase_N" evidence="8">
        <location>
            <begin position="5"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ83926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="868" mass="94208" checksum="B0BCC8DD1BC6824F" modified="2013-07-24" version="1">MANKSPYQQPLNDLYAAYHSGREGLSTAQANQNREQFGKNQLAQKKKKPVWMIFLEQFKDFLVIILIIAAIISAITGELESTIVILVVITMNAILGTVQTVKAEKSLDNLKSLSAPHAKVLRDGTTVLLPAAELVVGDVILLEAGDQVPADARILEAASLQTNESALTGESTNVEKECCEIPQEVPLGDRKNMVYSGGFVTYGRGVCLVTNVGMETEVGKIAALMQNASERRTPLQRTLDQFGQKLSIAILVISAIVFLLELFRVDVLNFDSIMNALMFAIALAVAAIPEALSSIVTIVLSFGTQKMAKEHAIMRKLQAVEGLGSVSVICSDKTGTLTQNKMTVRKIVAHGHSVAEEDVNLENDDEKWLIIASVLCSDATCQGETEIGDPTETALIRFSQKNGMQAEDLRSQYPRLAEIPFDSDRKLMSTLNQTPHGKILFTKGAADVLTERMLITTEEKEKIHKQVEALSKQGLRLLCFAGKPFDGDTISLEDETDLQYMGLIAMMDPPRPESAEAVAACKEAGIKPVMITGDHVVTAAAIAKEIGILQQDTEAVEGAVLDAYTDEQLVDFVKDKSVYARVTPEHKIRIVRAWQARNCMVAMTGDGVNDAPALKQADVGVAMGITGTEVSKDAAAMVLTDDNFATIVKAIENGRNLYENIKRAILFLLSGNLAGILVVLACALAGLGQPFAAIHLLFINLLTDSLPAIGLGLEPHQASVMRKKPRPANESILTKPFLISVGYSGLIIGIMTFLAYVLGRSEGGDYLGMTMAFATLCMSRLWHGFSCKSEEPVLFRKQMINNPFGLLAFFAGMILINAVLLVPPLKSLFSIASLTGLQYAWIHIFSFGSMILIQIVKFVKSMVQRSAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5VK85</accession>
    <name>R5VK85_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01670</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000174">
        <property type="protein sequence ID" value="CCZ83941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83941">
        <property type="protein sequence ID" value="CCZ83941"/>
        <property type="gene ID" value="BN566_01670"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="284" mass="29864" checksum="327DEFC95DA8E6C6" modified="2013-07-24" version="1">MKKQQIFAYACSAALLGTLIGCGQKTEIDQSVPDTIIEVAKTTASSETTSVTSETSAITSSSTGSATSSGSTNTTTATTIHYAGNPNLTVRRTEAPATQPTVAQTVTTIPTEAPTEPVTEAPTEATTIVETTVISTLVAPNGTFTEDDMRFTFDGISVKPGDSSIDFRNLMQGKGYVSTESVDGMQFISYDEQGIKLELVDNDDATLKAITLSNDKASTEKGIHVGSTKSDVEAAYGFGVEEDGVYRYLIDTNGDGENDYQLDFALDGNGVVQSITYCCITVGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>R5VKL3</accession>
    <name>R5VKL3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 14">Delta-aminolevulinic acid dehydratase</fullName>
            <ecNumber evidence="5 14">4.2.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">BN566_01757</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>2 5-aminolevulinate = H(+) + 2 H2O + porphobilinogen</text>
            <dbReference type="Rhea" id="RHEA:24064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58126"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="4.2.1.24"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 14">Homooctamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the ALAD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.24" evidence="5 14"/>
    <dbReference type="EMBL" id="CBAR010000180">
        <property type="protein sequence ID" value="CCZ84031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01757"/>
    <dbReference type="EnsemblBacteria" id="CCZ84031">
        <property type="protein sequence ID" value="CCZ84031"/>
        <property type="gene ID" value="BN566_01757"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00318"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004655">
        <property type="term" value="F:porphobilinogen synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001731">
        <property type="entry name" value="ALAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030656">
        <property type="entry name" value="ALAD_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11458">
        <property type="entry name" value="PTHR11458"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00490">
        <property type="entry name" value="ALAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001415">
        <property type="entry name" value="Porphbilin_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00144">
        <property type="entry name" value="DALDHYDRTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01004">
        <property type="entry name" value="ALAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00169">
        <property type="entry name" value="D_ALA_DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="8 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 14" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="11">
        <location>
            <position position="194"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="11">
        <location>
            <position position="247"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00020771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CCZ84031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="327" mass="36227" checksum="ACEA3DBF37E3E839" modified="2013-07-24" version="1">MNLIQRPRRLRNGSVLRSMVRETRISADTLIYPMFVIEGTNIKEEIATMPGQYRWSIDRMDEILQELTDAGVKSVLLFGIPKKKDEIGSSAWAEDGIVQQAIRHIKQQFPQLYVITDVCMCEYTSHGHCGILCGHSVDNDQTLEVLAKTALSHVQAGADMVAPSDMMDGRVGKIRAILDENGFSNTPIMSYSVKYASAFYGPFRDAAGSAPAFGDRKGYQMDPHNLQEAIREAELDIQEGADILMVKPGMAYLDVLYALKERFPYPIATYSVSGEYAMIKAAGMAGYVDEAALVCESAVCMYRAGADLLITYFAKELAQYIKEGRIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5VL86</accession>
    <name>R5VL86_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Germination protease</fullName>
            <ecNumber evidence="1">3.4.24.78</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">GPR endopeptidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Germination proteinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Spore protease</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gpr</name>
        <name evidence="2" type="ORF">BN566_00219</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Initiates the rapid degradation of small, acid-soluble proteins during spore germination.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endopeptidase action with P4 Glu or Asp, P1 preferably Glu &gt; Asp, P1' hydrophobic and P2' Ala.</text>
            <dbReference type="EC" id="3.4.24.78"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">Autoproteolytically processed. The inactive tetrameric zymogen termed p46 autoprocesses to a smaller form termed p41, which is active only during spore germination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A25 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.78" evidence="1"/>
    <dbReference type="EMBL" id="CBAR010000210">
        <property type="protein sequence ID" value="CCZ84306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00219"/>
    <dbReference type="EnsemblBacteria" id="CCZ84306">
        <property type="protein sequence ID" value="CCZ84306"/>
        <property type="gene ID" value="BN566_00219"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009847">
        <property type="term" value="P:spore germination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00626">
        <property type="entry name" value="Germination_prot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023430">
        <property type="entry name" value="Pept_HybD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005080">
        <property type="entry name" value="Peptidase_A25"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03418">
        <property type="entry name" value="Peptidase_A25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53163">
        <property type="entry name" value="SSF53163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0865">Zymogen</keyword>
    <feature type="chain" id="PRO_5023215264" description="Germination protease" evidence="1">
        <location>
            <begin position="1"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="propeptide" id="PRO_5009019594" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="281" mass="30233" checksum="F06F42B9C6CD4861" modified="2013-07-24" version="1">MSVRTDLAVESLTAAAAIEPKQRGTYFQITEVTISNAEQAAAVGKPEGHYLTLETAALHRHNPQFPQLAEELGRELRAFLPESGLVLVAGLGNRSIIPDALGVLVAERMFVTRHLQHTLSETDFEVFANLRPVSALTTGVMGQTGMESAELLAAVCEKTKPACVVVIDALACAALERLGCTIQICDSGIAPGSGVENCRKEISERTMQVPVVAIGVPTVVDLHTAAEGMLQQELPPMQQENWMVTPREIDELVQHAADLILCGLELALYPELSFEEVSALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5VLD4</accession>
    <name>R5VLD4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Chromosome segregation DNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00042</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000219">
        <property type="protein sequence ID" value="CCZ84361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00042"/>
    <dbReference type="EnsemblBacteria" id="CCZ84361">
        <property type="protein sequence ID" value="CCZ84361"/>
        <property type="gene ID" value="BN566_00042"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041468">
        <property type="entry name" value="HTH_ParB/Spo0J"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17762">
        <property type="entry name" value="HTH_ParB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH_ParB" evidence="1">
        <location>
            <begin position="6"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="126" mass="14285" checksum="9CE5CB554FA8192A" modified="2013-07-24" version="1">MQLPQPLQELLECGKITAGHARALLAISDPDLQLETARRCAEDGMTVRAVEKLAAEQAAKAEKPAKKRKTTDQYYKEMELELTDRLQRKVSVTYGRKKGTLMLEFYNKEDLASLAKQLTELAEQEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5VLH4</accession>
    <name>R5VLH4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Toxin-antitoxin system antitoxin component Xre family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00303</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000224">
        <property type="protein sequence ID" value="CCZ84396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00303"/>
    <dbReference type="EnsemblBacteria" id="CCZ84396">
        <property type="protein sequence ID" value="CCZ84396"/>
        <property type="gene ID" value="BN566_00303"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="12"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="112" mass="12877" checksum="2579FEB9D94747E4" modified="2013-07-24" version="1">MNLDYKQIGKNLARRRKELRLKQYEVCERAGINDKYLSCIETARSIPSLEVFLRLCEALETTPDHILLGSVCETDPTQSEAVLLEKFRSLSPKGQHLSLAFMNWIADELDCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5VM12</accession>
    <name>R5VM12_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Toxin secretion/phage lysis holin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_00422</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000246">
        <property type="protein sequence ID" value="CCZ84561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84561">
        <property type="protein sequence ID" value="CCZ84561"/>
        <property type="gene ID" value="BN566_00422"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006480">
        <property type="entry name" value="Phage_holin_4_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05105">
        <property type="entry name" value="Phage_holin_4_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01593">
        <property type="entry name" value="holin_tox_secr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ84561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="156" mass="16471" checksum="82CE670C55351616" modified="2013-07-24" version="1">MRETICTVLGVVGGTLAAAFGGWNAALTTLVFFLSLDYLTGLLVAGVFRRSPKTKTGGLQSKIGWKGLVKKVMVLLLVLAAARMDVALHTDYLRNTVCIGFTCNELISILENAGLMGIPLPAALKNAVDLLMQKGQGTTTKPSEKDSESSQGKGQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5VM42</accession>
    <name>R5VM42_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Metallophos domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00459</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000256">
        <property type="protein sequence ID" value="CCZ84606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00459"/>
    <dbReference type="EnsemblBacteria" id="CCZ84606">
        <property type="protein sequence ID" value="CCZ84606"/>
        <property type="gene ID" value="BN566_00459"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos" evidence="2">
        <location>
            <begin position="2"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="225" mass="26753" checksum="E139F05768C71A61" modified="2013-07-24" version="1">MVYVTGDLHGDYSRFKRPEMKKLRAGDILLVCGDFGFIWDNSKQEQAVLKKLSEKKYTIAFVDGCHENFDLLQQYRLVRWRGGVARLIAPNIFHLQRGEIYTIENQKYFAFGGGHSQDYEYRRDTGNWWRQEQPTHDEIRRAIRNLNRNQATVDYIITHEPPASLKDCLGVDVQQRLEIHAFFEDIIKECDYKKWFFGKCHMDRFIPMKFYAVFQDVLPVTDERN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5VM50</accession>
    <name>R5VM50_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00469</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000257">
        <property type="protein sequence ID" value="CCZ84616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84616">
        <property type="protein sequence ID" value="CCZ84616"/>
        <property type="gene ID" value="BN566_00469"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="95" mass="10818" checksum="130201C1270B05DB" modified="2013-07-24" version="1">MTQKELRKLKKTELLEMMIALRNELDRVQAERDALQEQLKTAEQNALHQVETDAVLAVQKILKAVEDETAAYRKRIQAESKETKSASPEVPADSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>R5VM83</accession>
    <name>R5VM83_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Protein-(Glutamine-N5) methyltransferase release factor-specific</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_00509</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Methylates the class 1 translation termination release factors RF1/PrfA and RF2/PrfB on the glutamine residue of the universally conserved GGQ motif.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000266">
        <property type="protein sequence ID" value="CCZ84656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00509"/>
    <dbReference type="EnsemblBacteria" id="CCZ84656">
        <property type="protein sequence ID" value="CCZ84656"/>
        <property type="gene ID" value="BN566_00509"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004556">
        <property type="entry name" value="HemK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040758">
        <property type="entry name" value="PrmC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019874">
        <property type="entry name" value="Release_fac_Glu-N5_MeTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17827">
        <property type="entry name" value="PrmC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00536">
        <property type="entry name" value="hemK_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03534">
        <property type="entry name" value="RF_mod_PrmC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PrmC_N" evidence="6">
        <location>
            <begin position="9"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="MTS" evidence="5">
        <location>
            <begin position="115"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF17827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ84656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="283" mass="31154" checksum="3E2E1CDE51104346" modified="2013-07-24" version="1">MVTMTRRTLYQTIRQRLQAAGIEDAGIESGFLLEFLLGKPLPQLLMDGELPVPEAIVMQAETLCQRRAVHYPLQYLFGSWEFYGLSFEIGEGVLIPRADTETLVDVVCTLRSGQPQTRLLDLCSGSGCIPAAIATQLPEVSGAAIERESAAFSYLQKNLQQYAPQIQPVQGDVLDATLPDLYTGYDVITCNPPYLTEVDMQQLQPEVTFEPETALYGGADGLDFYRNISANWGSALRKSGWLVYEIGKGQEQDVAAILRQQGFSQIIQQTDGNGIVRVVAGQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>R5VM93</accession>
    <name>R5VM93_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Alpha-1,4 glucan phosphorylase</fullName>
            <ecNumber evidence="10">2.4.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN566_00519</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Allosteric enzyme that catalyzes the rate-limiting step in glycogen catabolism, the phosphorolytic cleavage of glycogen to produce glucose-1-phosphate, and plays a central role in maintaining cellular and organismal glucose homeostasis.</text>
    </comment>
    <comment type="function">
        <text evidence="8">Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>[(1-&gt;4)-alpha-D-glucosyl](n) + phosphate = [(1-&gt;4)-alpha-D-glucosyl](n-1) + alpha-D-glucose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:41732"/>
            <dbReference type="Rhea" id="RHEA-COMP:9584"/>
            <dbReference type="Rhea" id="RHEA-COMP:9586"/>
            <dbReference type="ChEBI" id="CHEBI:15444"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="EC" id="2.4.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 10">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the glycogen phosphorylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.1" evidence="10"/>
    <dbReference type="EMBL" id="CBAR010000267">
        <property type="protein sequence ID" value="CCZ84666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00519"/>
    <dbReference type="EnsemblBacteria" id="CCZ84666">
        <property type="protein sequence ID" value="CCZ84666"/>
        <property type="gene ID" value="BN566_00519"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008184">
        <property type="term" value="F:glycogen phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102250">
        <property type="term" value="F:linear malto-oligosaccharide phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102499">
        <property type="term" value="F:SHG alpha-glucan phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011833">
        <property type="entry name" value="Glycg_phsphrylas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000811">
        <property type="entry name" value="Glyco_trans_35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035090">
        <property type="entry name" value="Pyridoxal_P_attach_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11468">
        <property type="entry name" value="PTHR11468"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00343">
        <property type="entry name" value="Phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000460">
        <property type="entry name" value="Pprylas_GlgP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02093">
        <property type="entry name" value="P_ylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00102">
        <property type="entry name" value="PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6 9 10" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="9">
        <location>
            <position position="657"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000460-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ84666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="801" mass="90937" checksum="99EFE8A8DFD4CD9E" modified="2013-07-24" version="1">MEKKQLTQKEELKQQFIDRLQLDCSVSPDEASDSQIYQVLSAMMVQRLKYMRQHFVNKTNSIGGKKVYYLSMEFLMGRSLKTTLYNLELVDDVTSILNDYHININQIYEQEPDAGLGNGGLGRLAACYLDAMATQGIPATGHSICYEYGIFRQELQDGWQTEFPDNWLPGGSVWLDPKPDEAIEVHFEGEIQEYWQDSYHYLSHHNYNTVLAIPYDLYVSGYGSEGVSALRLWQAKAPSFDMKSFNEGNYASALSQNSMANAISKVLYPNDNHLEGKSLRLRQQYFLSAAAIGDIVNHHMSVYGTLDNLHEKVAIHINDTHPTLAIPELMRVLLDDCGYTWDHAWHIVTNTFAYTNHTVMAEALEKWDVNLVKQIIPRIFQIIVEINNRYCASLMERNGGDSAKTTRMSIIKDNQIHMATLCVMASHSVNGVSKLHSEIIKESVFHDEYLDTPNKFKNVTNGIAYRRWLYQSNPGLTKLLREKIGNKFLVDGSELKKLDVFENDKAVLEQLRKIKKENKERFAKYVKQKSGVLLNTDSIFDVQVKRLHEYKRQHLNVMNILAQYQYLLDHPNADFAPKTYIFAAKAAPGYYLAKQIIKMIWALSEEIRKNPRISEKLSVYFLEDYKVTLSELLMPASDISEQISLAGTEASGTGNMKLMLNGAITLGTLDGANIEIGDAVGPDNILIFGMKTEEVNALKAQGYHPGAYYEQNPIIHSCIDRMYAGINGCKFAEVADSLKNIDPYMVLADFDSYAAIQKKSSELYQDTEKWTKMSLHNIAGAGIFSADRAVNEYARDIWGVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5VQD9</accession>
    <name>R5VQD9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00607</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000008">
        <property type="protein sequence ID" value="CCZ82802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82802">
        <property type="protein sequence ID" value="CCZ82802"/>
        <property type="gene ID" value="BN566_00607"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046313">
        <property type="entry name" value="DUF6465"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20069">
        <property type="entry name" value="DUF6465"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="86" mass="9297" checksum="E13FD65E0B7E6124" modified="2013-07-24" version="1">MPKQTTTEKAAATPAKKTASRKKVVFTLQFAGKELTVAEMEALAVAAWMEQTGGLKKDAKDIQLYAKPEDSMLYYVINGESGSVAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5VQZ6</accession>
    <name>R5VQZ6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00772</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000034">
        <property type="protein sequence ID" value="CCZ82972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00772"/>
    <dbReference type="EnsemblBacteria" id="CCZ82972">
        <property type="protein sequence ID" value="CCZ82972"/>
        <property type="gene ID" value="BN566_00772"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042204">
        <property type="entry name" value="2Fe-2S-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="SSF54292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="96" mass="10552" checksum="5379F999D9E529CA" modified="2013-07-24" version="1">MGLSKKVKFQYNGTELEGYEGEPIAMALKAAGVMVHRYTQKEHQPRGIFCAIGRCTDCVMIVNGKPNVRTCVTPLEAGMQVETQYGVSAEPFSKQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>R5VR56</accession>
    <name>R5VR56_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Mini-ribonuclease 3</fullName>
            <shortName evidence="2">Mini-3</shortName>
            <shortName evidence="2">Mini-RNase 3</shortName>
            <ecNumber evidence="2">3.1.26.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Mini-RNase III</fullName>
            <shortName evidence="2">Mini-III</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">mrnC</name>
        <name evidence="4" type="ORF">BN566_00810</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Involved in correct processing of both the 5' and 3' ends of 23S rRNA precursor. Processes 30S rRNA precursor transcript even in absence of ribonuclease 3 (Rnc); Rnc processes 30S rRNA into smaller rRNA precursors.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MrnC RNase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.-" evidence="2"/>
    <dbReference type="EMBL" id="CBAR010000038">
        <property type="protein sequence ID" value="CCZ83017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00810"/>
    <dbReference type="EnsemblBacteria" id="CCZ83017">
        <property type="protein sequence ID" value="CCZ83017"/>
        <property type="gene ID" value="BN566_00810"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00593">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01468">
        <property type="entry name" value="RNase_Mini_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008226">
        <property type="entry name" value="Mini3_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00636">
        <property type="entry name" value="Ribonuclease_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005520">
        <property type="entry name" value="UCP005520"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00535">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="SSF69065"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 2" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="RNase III" evidence="3">
        <location>
            <begin position="6"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="144" mass="15890" checksum="7C2D96A8B863C4D9" modified="2013-07-24" version="1">MTQTPLSPHDAKQYSPLSLAFLGDSVFDTLIREQLLRTANQPPAALHQKKVALVCAGFQATVMRELLPTLTEEEQAVFLRGRNAAPKHAHQAEYRYATGLEALFGYLYLTGAADRMQELMQQIRQTAAFQAVLTADAAENQSKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5VR80</accession>
    <name>R5VR80_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted membrane protein involved in D-alanine export</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00845</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000043">
        <property type="protein sequence ID" value="CCZ83052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00845"/>
    <dbReference type="EnsemblBacteria" id="CCZ83052">
        <property type="protein sequence ID" value="CCZ83052"/>
        <property type="gene ID" value="BN566_00845"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="176"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="210"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="299"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="336"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="381"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="414"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="450" mass="51718" checksum="699C13B70AA613E2" modified="2013-07-24" version="1">MFLNFLFLLILFPLSLLVYHCVPQRWQQPVLLGTSILFLVLWQAECSLLFFGLIFLTGLFSQLLAHLSPRTLPARLLLLFHDSIYIAALFVLRPPHEGHLFGMALYPVGISILALQSISYVHLIFRRAILPERHRSSFAFYFCFYPKLLFGPYCSVSTFHRIQPDRRCQVEQVGNGLHQLVCGLAKAVLLSGQFYLIIQQMQAASKEHNTLFFSWLYQLLFFLYVYFQITGYTDMARGIAACYGYALPKSSRLPLWNKQLSVFLQNWNRTVLRWFSRAFSSPKQTAASLWLQMLFTYSVLGWFCRGTLTGFLGGLLIGVILAWFEDVQRKWKFPTAIHWFLSIICSVFAWSLFCSDTLTEAVQTWGNLLGLSKDAVSYQDLYFLQSSLLLLLIAGFCTSGWHRSIQKWLKKRTLTAWIPTIVVPVGDVLFLGLSVLAMASNAIPPLFFRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5VRG2</accession>
    <name>R5VRG2_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cupin_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00934</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000064">
        <property type="protein sequence ID" value="CCZ83147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00934"/>
    <dbReference type="EnsemblBacteria" id="CCZ83147">
        <property type="protein sequence ID" value="CCZ83147"/>
        <property type="gene ID" value="BN566_00934"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin_2" evidence="1">
        <location>
            <begin position="33"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="118" mass="12798" checksum="7ED956CF12BABAFF" modified="2013-07-24" version="1">MHNFTKVHVAQDARTELHDKLSLTGAEISVNNLPAGAGVPFVHSHKKNEEIYIILSGQGKAVIDGETVELHAGDFVRVAPAAKRQFFAGDDSAISFVCIQVQEHSLEEYTAEDAVIAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5VRL7</accession>
    <name>R5VRL7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Bacterial sugar transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00985</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial sugar transferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000070">
        <property type="protein sequence ID" value="CCZ83202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00985"/>
    <dbReference type="EnsemblBacteria" id="CCZ83202">
        <property type="protein sequence ID" value="CCZ83202"/>
        <property type="gene ID" value="BN566_00985"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003362">
        <property type="entry name" value="Bact_transf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02397">
        <property type="entry name" value="Bac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="46"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="Bac_transf" evidence="3">
        <location>
            <begin position="42"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="235" mass="27607" checksum="A48CC7AFF2FAAD2F" modified="2013-07-24" version="1">MQTEWIWAAKANQAILTEKRSQKVWLKQELINKKRVYAVFQRSRDIFFSLLAIIVLSPLMLLTALLIVLDDPHGSPIFSQYRVGKNGRKFKLYKFRSMVVHAEDNLESLMQQNEMDGPVFKIKKDPRITRIGHFIRKTSIDELPQLFNILKGEMSIVGPRPSLPREVEKYTPYQMQRLLVTPGLTCYWQIQPQRNSLSFEEWVALDIQYIQDRSYWLDWKIIFKTVGAVLTCQGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5VRQ4</accession>
    <name>R5VRQ4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Serine/threonine protein phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01018</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000081">
        <property type="protein sequence ID" value="CCZ83237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01018"/>
    <dbReference type="EnsemblBacteria" id="CCZ83237">
        <property type="protein sequence ID" value="CCZ83237"/>
        <property type="gene ID" value="BN566_01018"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006470">
        <property type="term" value="P:protein dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PTHR13832"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="SSF81606"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="1">
        <location>
            <begin position="8"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="265" mass="29790" checksum="64C3A97CC7F09976" modified="2013-07-24" version="1">MIVLHKLHATVLTDQGKVRTNNEDNFYFDGKGYEEPQAFCHFSEKCSTRKKPIFAVFDGMGGESYGEIASSLAVQTLRQHEKAIKHCKSEQLDAVVQEFVADANQNIYQMAKKLQGGNSGTTMAMLCFQGDQVYPFYIGDSRIYLYNGTDLYRLTSDHTLANHKLQTGIYTKEEAELSSDRNKLIQFLGADLQHFTLPHAAQPPLRIYRGYRFLLCSDGLTDMCDDQTIYRVLSSGQPDMANELMQLAMDAGGVDNTTLILIEVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>R5VRT3</accession>
    <name>R5VRT3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Sulfate adenylyltransferase</fullName>
            <ecNumber evidence="1">2.7.7.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_01053</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.4" evidence="1"/>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01053"/>
    <dbReference type="EnsemblBacteria" id="CCZ83272">
        <property type="protein sequence ID" value="CCZ83272"/>
        <property type="gene ID" value="BN566_01053"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004781">
        <property type="term" value="F:sulfate adenylyltransferase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006790">
        <property type="term" value="P:sulfur compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04166">
        <property type="entry name" value="CysN_ATPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03695">
        <property type="entry name" value="CysN_NodQ_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04095">
        <property type="entry name" value="CysN_NoDQ_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041757">
        <property type="entry name" value="CysN_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044138">
        <property type="entry name" value="CysN_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044139">
        <property type="entry name" value="CysN_NoDQ_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011779">
        <property type="entry name" value="SO4_adenylTrfase_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="SSF50465"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02034">
        <property type="entry name" value="CysN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tr-type G" evidence="7">
        <location>
            <begin position="2"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="561" mass="62395" checksum="2538B12D58BB59EF" modified="2013-07-24" version="1">MKGLLKFITCGSVDDGKSTLIGHILYDAKLLYADQEKALELDSKVGSRSGKIDYSLLLDGLMAEREQGITIDVAYRYFTTDNRSFIVADTPGHEEYTRNMAVGASFADLAVILIDASQGVLVQTRRHARICKLMGIRYFVFAVNKMDLVNYSESRFNEIVAQIDALKQELGLENITIIPLSATEGDNVTTHSENIPWYTGKPLLEYLETVDIASDTEEGFYMPVQRVCRPDHTFRGFQGQIESGSISVGDAITTLPSNETATVKGILVTDKEATSAKQGQPVTITLDREVDVSRGCVLVKNTDIGVYKKLTVSLLWMDDEDLAIGKDYLVKLGTKTIPGILTHVQYAIDVNTGEHVTKDSLHKNEIAVCELLLTEGIVVDLFAKHKTLGELILIDRVTNMTSACGVVEAVAEKVTNGTEKASFRLNDLTARGDIFEEFYYDTSSLNVLKYQPVNRTYTIGDEIPVSGESYQYPDSFDIIVLRDQVAVRVRNKKITAILPAADYVYDHVPVINGRGFAVLADSNEQVQQFLQEYAATNEADQTAFFAKWVRFDTYRKVIFPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>R5VSV1</accession>
    <name>R5VSV1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Trypsin-like serine proteases typically periplasmic contain C-terminal PDZ domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_00139</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000146">
        <property type="protein sequence ID" value="CCZ83612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00139"/>
    <dbReference type="EnsemblBacteria" id="CCZ83612">
        <property type="protein sequence ID" value="CCZ83612"/>
        <property type="gene ID" value="BN566_00139"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001940">
        <property type="entry name" value="Peptidase_S1C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13180">
        <property type="entry name" value="PDZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00834">
        <property type="entry name" value="PROTEASES2C"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="SSF50156"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="SSF50494"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="105"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="4">
        <location>
            <begin position="420"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="143"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="11"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="528" mass="55313" checksum="A2EBED8407027997" modified="2013-07-24" version="1">MYEREQDMMKNESNNTAASASNQPESTVNNQNPYSTSAATGSTTPNGYGYTTNSSTNPTTGYTGNTSSDTQSQQQGYSYVNYYSNPNAPVQASGSGKPPKKKNHFWMKAVAFVAAMAIVSVGSIGIYNGVHNSNKLVAAESSTSASDSTDTTANTTAQDSSSKAQSTTVKGDAAKSWIELASGTNAMSISDIVKKVAPSVVGVQATFKMPNNYNSYNYGFGMFGNGNSGNGDNSGSMTGVGTGIIMREDGYIVTNAHVIYDSEYGCGEATAVEIRMSDEETTYDAKVVAYDIETDLAVLKVDQTGLTAAEFGDSDQCQVGDAVVAIGNPLGLELQNTVTCGIISALNRKVTINDKTMTLIQTDTAINNGNSGGPLINSSGQVVGINSAKMSSSVSSSGATIEGIGFAIPMTEAREIVDDLINYGYVTGRPQLGISCQDVSEAVSQAYNLPIGAYVISVTDGGAAAEAGLQVGDVITAINDNKIETTEELNNYKNEYNAGDTVTLTIVRNGQEQKVSVVLQEVQQKKQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>R5VT39</accession>
    <name>R5VT39_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulators</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_01438</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000154">
        <property type="protein sequence ID" value="CCZ83702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01438"/>
    <dbReference type="EnsemblBacteria" id="CCZ83702">
        <property type="protein sequence ID" value="CCZ83702"/>
        <property type="gene ID" value="BN566_01438"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="159" mass="17776" checksum="88665253452E7911" modified="2013-07-24" version="1">MQKLISLLQQNARLSNAQLAVMLGKTEEEVAADIRKLEEDGIIKGYSVILNEEAINKDMVTAIVELRVTPQRDCGFESIARTIMQYDEVESISLMAGAYDFAVTVKGANVKDVSLFVSQRLAPLGNVLSTATYFVLKKYKEMGKIVVDEEKDERGFYFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5VT70</accession>
    <name>R5VT70_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01472</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="3">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000159">
        <property type="protein sequence ID" value="CCZ83737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01472"/>
    <dbReference type="EnsemblBacteria" id="CCZ83737">
        <property type="protein sequence ID" value="CCZ83737"/>
        <property type="gene ID" value="BN566_01472"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011863">
        <property type="entry name" value="HSK-PSP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02137">
        <property type="entry name" value="HSK-PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="90"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="7"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="9"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR611863-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR611863-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR611863-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="201" mass="22645" checksum="24FB895EF49EEA61" modified="2013-07-24" version="1">MYITCLDLEGVLVPEIWIAFAEESGIPELKRTTRDEPDYDKLMKWRIGVLKEHGLGLKEIQETIAKIDPLPGAKEFLDKLRKECQVIIISDTFTQFAGPLMEKLGQPTIFCNSLEVAADGEITGFKMRVENSKLTTVKALQSIGYDTIASGDSYNDLAMIQASKAGFLFRSTEQIQKDHPELPAFERYEDLLAAIEQELQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5VTF7</accession>
    <name>R5VTF7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01552</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000165">
        <property type="protein sequence ID" value="CCZ83817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01552"/>
    <dbReference type="EnsemblBacteria" id="CCZ83817">
        <property type="protein sequence ID" value="CCZ83817"/>
        <property type="gene ID" value="BN566_01552"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="204" mass="22921" checksum="C44819DF78538D48" modified="2013-07-24" version="1">MSNRIITIEREYASGGREVGELVAKQLGIPFYNREILEMASERCGVSVDYLASAEEAAPKSFLYTLMLSSNPTRSIQENLPLSDKVYIIETNIIKELAEKSDCVIVGRCASYILRDMEHMMSAFVYADQESRVNRAVSEYSVDPRRAEATLRKSDRRRETFYSINTGGNWYDKENYTLCLNSGALGIPLCADIIVSAAQKTIFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>R5VU15</accession>
    <name>R5VU15_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">ATP phosphoribosyltransferase regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">hisZ</name>
        <name evidence="8" type="ORF">BN566_01726</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 6">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heteromultimer composed of HisG and HisZ subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="6">This function is generally fulfilled by the C-terminal part of HisG, which is missing in some bacteria such as this one.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000178">
        <property type="protein sequence ID" value="CCZ83997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01726"/>
    <dbReference type="EnsemblBacteria" id="CCZ83997">
        <property type="protein sequence ID" value="CCZ83997"/>
        <property type="gene ID" value="BN566_01726"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00006"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00773">
        <property type="entry name" value="HisRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00125">
        <property type="entry name" value="HisZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041715">
        <property type="entry name" value="HisRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004516">
        <property type="entry name" value="HisRS/HisZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004517">
        <property type="entry name" value="HisZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43707">
        <property type="entry name" value="PTHR43707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13393">
        <property type="entry name" value="tRNA-synt_His"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001549">
        <property type="entry name" value="His-tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00443">
        <property type="entry name" value="hisZ_biosyn_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II" evidence="7">
        <location>
            <begin position="1"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="398" mass="44646" checksum="59FD7F81A147B02D" modified="2013-07-24" version="1">MRNYNRITPEGTKDLLLEECLVRRETEEKIRSVFQSQGYDELVTPGLEFFDVFQSEAVQYPQEQLYKLTDSKGRLIVLRPESTAPIARVVATRLREAMLPQRLCYMQTAYRFEPALKGRSDEFMQAGAELIGSDSYMADLEMASMAIDVLKRCNETSYYLELGDAGIFKELMRELNAAPATKEEIRYLIETKNYSALNDLLDTLGDNQLIHALKKLPSLFGGEEVFDKMEQLYCTPQIQEMLQYLRKLYREIAALIGTERLSVDLGIVNKADYYTGMILKGYLEGYGDEVLSGGRYNKLLASFGYDVPAVGFAVNVDAVAKVAMKKNPRTKKAADAAIYAAPGYEAAAIALAETKREAGLTVEFALVSSAEAALDYAKNRKISTLYQVEKEVTEVTVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>R5VU29</accession>
    <name>R5VU29_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 10 12">1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase</fullName>
            <ecNumber evidence="5 10 12">5.3.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">hisA</name>
        <name evidence="13" type="ORF">BN566_01731</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10 12">
            <text>1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide = 5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:15469"/>
            <dbReference type="ChEBI" id="CHEBI:58435"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="5.3.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 10 12">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 4/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10 11">Belongs to the HisA/HisF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.16" evidence="5 10 12"/>
    <dbReference type="EMBL" id="CBAR010000178">
        <property type="protein sequence ID" value="CCZ84002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01731"/>
    <dbReference type="EnsemblBacteria" id="CCZ84002">
        <property type="protein sequence ID" value="CCZ84002"/>
        <property type="gene ID" value="BN566_01731"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00009"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003949">
        <property type="term" value="F:1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04732">
        <property type="entry name" value="HisA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01014">
        <property type="entry name" value="HisA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006062">
        <property type="entry name" value="His_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006063">
        <property type="entry name" value="HisA_bact_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044524">
        <property type="entry name" value="Isoase_HisA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023016">
        <property type="entry name" value="Isoase_HisA-like_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43090">
        <property type="entry name" value="PTHR43090"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00977">
        <property type="entry name" value="His_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00007">
        <property type="entry name" value="TIGR00007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 10 11" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="9 10 12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="8"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CCZ84002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="242" mass="25412" checksum="CCC0CE16A128BD42" modified="2013-07-24" version="1">MIILPAIDIQDGTCVRLVKGDFATSHKVAEDYRKTAAGFVAAGASWIHMVDLDGARAGEPKNADLFLDVAKNTPLKVELGGGIRSLDTVEAYLKGGISRVILGSIALKNPQIVKDAVKEYGERIAVGIDAKNGMVAVEGWLDSSSVDYLSLAKEMAAVGVQYIIYTDISRDGTLSGVNTEQLQQIHQAVGAGCNIIASGGVHTIADIQACKQMGLYGTICGKSLYQGTLQLEDAIACAGAQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5VUE5</accession>
    <name>R5VUE5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Stage IV sporulation protein A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01862</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000192">
        <property type="protein sequence ID" value="CCZ84137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01862"/>
    <dbReference type="EnsemblBacteria" id="CCZ84137">
        <property type="protein sequence ID" value="CCZ84137"/>
        <property type="gene ID" value="BN566_01862"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043934">
        <property type="term" value="P:sporulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014201">
        <property type="entry name" value="Spore_IV_A"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09547">
        <property type="entry name" value="Spore_IV_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02836">
        <property type="entry name" value="spore_IV_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="424" mass="47277" checksum="E099035DC125D6EA" modified="2013-07-24" version="1">MTTEPKFIPEEAAEITIGEHTHFRIRMVDCVGYILPSALGYIENDMPRMVKTPWFEEEVPFGMAAEVGTQKVITEHATVGLVVTTDGSITNLPREEYAACEERVIQELQQLEKPFIVLVNAIEPHSRQTEALCESLRKKYHAAVLPVCCPELEEADIQEILTQLLYAFPIREVQIQTAGWITALDPEHWLRKSVFSSIRAAAEPLRYVRDVPMMTETLAGAEHILSASVQQIDLGTGRAVVSVTMNGSLFYQILGETTGLQIASESDLFPVLTELCKIKKSYDRIAPALEAVEATGYGIVMPQLEEMSLEEPEIIKQGGKYGVRLRASAPSIHMMKASIRTAVSPIVGTEKQSEELVMYLLEGFEEDPTKIWSSNIFGKSLHELVNEGLHSKLSKMPMEARLKLQETLERVINEGCSGLICFIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5VUQ8</accession>
    <name>R5VUQ8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MurNAc-LAA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00186</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000202">
        <property type="protein sequence ID" value="CCZ84257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00186"/>
    <dbReference type="EnsemblBacteria" id="CCZ84257">
        <property type="protein sequence ID" value="CCZ84257"/>
        <property type="gene ID" value="BN566_00186"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008745">
        <property type="term" value="F:N-acetylmuramoyl-L-alanine amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009253">
        <property type="term" value="P:peptidoglycan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02696">
        <property type="entry name" value="MurNAc-LAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002508">
        <property type="entry name" value="MurNAc-LAA_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01520">
        <property type="entry name" value="Amidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004395733" description="MurNAc-LAA domain-containing protein" evidence="1">
        <location>
            <begin position="25"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="MurNAc-LAA" evidence="2">
        <location>
            <begin position="78"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="365" mass="40602" checksum="2573C15ECA394C92" modified="2013-07-24" version="1">MNRFSLVAATLALSIFCTPNVASATNSLPQTPSEWMDFLEKQSINQEDYGNSDQNFVTDSLPLLKDSAQTPDPNVIKIMIDPGHANYYNPSTVVNGYYESVMTWTLSNYLKEELEALGVQADLTKTSLEDDPDLQPRGHMSAGYDFFLSVHSNATSYSSIDYPVAICYQNLDWTDIDDTSRAVGQLLTDKVTEVMETRQKGIIWQRLSDNDRDGNGVNDDEWYGVLCGARYVGTPGVLMEHSFHTNYRATVWLMQDSNLRKLAKEEANVLYTYFRTQKESNRYIGDVDGDGSLSVQDAVQILTYYAQQAAGCSPTFASDLQYTTADYDGDDSITVNDAVSVLETYAKQAAGLQPTLLMVGDRAHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5VV39</accession>
    <name>R5VV39_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00289</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000222">
        <property type="protein sequence ID" value="CCZ84382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00289"/>
    <dbReference type="EnsemblBacteria" id="CCZ84382">
        <property type="protein sequence ID" value="CCZ84382"/>
        <property type="gene ID" value="BN566_00289"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="SSF47336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="91" mass="10557" checksum="DAAEA4DA5C0B3960" modified="2013-07-24" version="1">MTHAEVMERLTGVFRDVFDDDSIVITDQTTANDIEEWDSLEHINLIDAVEQEFHMQFQMKEVSGMKNVGEMADIVANRATEKPKKRGLFRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVP7</accession>
    <name>R5VVP7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_00448</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000248">
        <property type="protein sequence ID" value="CCZ84587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00448"/>
    <dbReference type="EnsemblBacteria" id="CCZ84587">
        <property type="protein sequence ID" value="CCZ84587"/>
        <property type="gene ID" value="BN566_00448"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003810">
        <property type="entry name" value="Mntp/YtaF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35529">
        <property type="entry name" value="PTHR35529"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02659">
        <property type="entry name" value="Mntp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="57"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="178"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ84587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="196" mass="20557" checksum="E30769834A298C7F" modified="2013-07-24" version="1">MLQILLPAWTVCLDLFFTAIAYGISGIRIPVRAACILSGTGAGCFLAAMELAKWSGLFLPASFCKLAGVVLLAAMGGAMLLKSFVEPADSSEKSANFRTVYFDAACADADHSRSLSAREAFLLAAALSVDSLAVGVGIGWQDSMPVAIAGATLLFGILGVLCGTKIGVRLHRRQKRDYSWLSGACLLLLAVLQAVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVU2</accession>
    <name>R5VVU2_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phosphomethylpyrimidine synthase</fullName>
            <ecNumber evidence="10">4.1.99.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Hydroxymethylpyrimidine phosphate synthase</fullName>
            <shortName evidence="10">HMP-P synthase</shortName>
            <shortName evidence="10">HMP-phosphate synthase</shortName>
            <shortName evidence="10">HMPP synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Thiamine biosynthesis protein ThiC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">thiC</name>
        <name evidence="11" type="ORF">BN566_00495</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + S-adenosyl-L-methionine = 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + 5'-deoxyadenosine + CO + formate + 3 H(+) + L-methionine</text>
            <dbReference type="Rhea" id="RHEA:24840"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:17245"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58354"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="EC" id="4.1.99.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="10">Binds 1 [4Fe-4S] cluster per subunit. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Cofactor biosynthesis; thiamine diphosphate biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the ThiC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.17" evidence="10"/>
    <dbReference type="EMBL" id="CBAR010000257">
        <property type="protein sequence ID" value="CCZ84642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00495"/>
    <dbReference type="EnsemblBacteria" id="CCZ84642">
        <property type="protein sequence ID" value="CCZ84642"/>
        <property type="gene ID" value="BN566_00495"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00060"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016830">
        <property type="term" value="F:carbon-carbon lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00089">
        <property type="entry name" value="ThiC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037509">
        <property type="entry name" value="ThiC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038521">
        <property type="entry name" value="ThiC/Bza_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002817">
        <property type="entry name" value="ThiC/BzaA/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30557">
        <property type="entry name" value="PTHR30557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01964">
        <property type="entry name" value="ThiC_Rad_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00407">
        <property type="entry name" value="phosphomethylpyrimidine_syntha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00190">
        <property type="entry name" value="thiC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 10" id="KW-0408">Iron</keyword>
    <keyword evidence="8 10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 10" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="5 10" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="10">
        <location>
            <begin position="186"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="10">
        <location>
            <begin position="227"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="334"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="410"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="413"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="417"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ84642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="441" mass="48942" checksum="2C9EAE829CD233DE" modified="2013-07-24" version="1">MQTYHTQMEAARKHIITPQMKTVAEKERVDVEWLRERVAAGTICIPANIHHTSLSAEGIGTGLRTKINVNLGISGDCHDYTDEMKKVDLAIQFGCEAIMDLSNYGKTSTFRKQLIEKSPAMIGTVPMYDAIGYLEKDLKEIKAEDFLRVIEAHAKEGVDFQTIHAGINRRAVEALKRQKRLTNVVSRGGSLIFAWMEMTGNENPFYEYYDEVLEILRTYDVTISLGDALRPGSTADSTDAAQISELIELGDLTKRAWEKDVQVMVEGPGHMAMNEIAANMALQKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGAIAASSGANFLCYVTPAEHLRLPDLQDVREGIVASKIAAHAADIANGIPYAREQDNRMSEARQRIDWEGMFACAIDPEKARNYFESRPPQERHTCSMCGKMCAMRTSNRILNGEDVTFCEADSEQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5UX41</accession>
    <name>R5UX41_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC-type antimicrobial peptide transport system ATPase component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_00680</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000020">
        <property type="protein sequence ID" value="CCZ82878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00680"/>
    <dbReference type="EnsemblBacteria" id="CCZ82878">
        <property type="protein sequence ID" value="CCZ82878"/>
        <property type="gene ID" value="BN566_00680"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="3"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="39"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ82878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="232" mass="25803" checksum="91B0919D21ABF492" modified="2013-07-24" version="1">MFLEISSIKKHFGEGESRVEVLKGIDISIEKGEICVLLGPSGSGKSTLLNIIGGIDNADSGYISIDGEKTADMNEKTLTLYRRKHLGYIFQMYNLIPNLNIKENVEVGAYLSDHPLDVDDLLKTLGLYEHRHKLPNQLSGGQQQRTAIGRAIVKNPDILLCDEPTGALDYHTSKEILSLIETVNKKYGNTVIMVTHNDAIKNMADRVVQLRDGMIRTNTVNQTKIPAAELEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5UX48</accession>
    <name>R5UX48_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase</fullName>
            <ecNumber evidence="3">4.2.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_00685</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the prolyl-tRNA editing family. YbaK/EbsC subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.-.-" evidence="3"/>
    <dbReference type="EMBL" id="CBAR010000022">
        <property type="protein sequence ID" value="CCZ82883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00685"/>
    <dbReference type="EnsemblBacteria" id="CCZ82883">
        <property type="protein sequence ID" value="CCZ82883"/>
        <property type="gene ID" value="BN566_00685"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00002">
        <property type="entry name" value="YbaK_deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.960.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004369">
        <property type="entry name" value="Prolyl-tRNA_editing_YbaK/EbsC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036754">
        <property type="entry name" value="YbaK/aa-tRNA-synt-asso_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007214">
        <property type="entry name" value="YbaK/aa-tRNA-synth-assoc-dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04073">
        <property type="entry name" value="tRNA_edit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006181">
        <property type="entry name" value="EbsC_YbaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55826">
        <property type="entry name" value="SSF55826"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00011">
        <property type="entry name" value="YbaK_EbsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="tRNA_edit" evidence="4">
        <location>
            <begin position="36"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR006181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ82883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="161" mass="17555" checksum="AED4E1F2A67E08E3" modified="2013-07-24" version="1">MAKEKLEKTNAIRILEREKVSYQHRSWSAATKENTGVELAQALGQPPEQVFKTLVTVGKSGNHYVFMLPVAETLHLKKAAAAVGEKAVSMIKEKELLPLTGYVHGGCSPIGMKKLFPTVIHESALQFDTILCSAGKIGHQLEVSPLDIQKVTGCTFADIIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXJ0</accession>
    <name>R5UXJ0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative DNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00595</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000008">
        <property type="protein sequence ID" value="CCZ82790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82790">
        <property type="protein sequence ID" value="CCZ82790"/>
        <property type="gene ID" value="BN566_00595"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.960.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036754">
        <property type="entry name" value="YbaK/aa-tRNA-synt-asso_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ82790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="156" mass="17827" checksum="4765BF68D92CA19D" modified="2013-07-24" version="1">MNSTALQIYSFFMRTNLQFVRYCHPAYTNRNQWKSLLKKSKLEATMPVCSVIPHQSGYALYLHPEETPAPSGSESLTAACCTLLHCLPHTLSPLGLLFDAQNQCPLYVHPDFQSVSHWWFSPCSDVSSVFLSQNVFLEQFLPQIHHPIYWESGFDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXN1</accession>
    <name>R5UXN1_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Lipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit MurT</fullName>
            <ecNumber evidence="1">6.3.5.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">murT</name>
        <name evidence="4" type="ORF">BN566_00836</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The lipid II isoglutaminyl synthase complex catalyzes the formation of alpha-D-isoglutamine in the cell wall lipid II stem peptide. The MurT subunit catalyzes the ATP-dependent amidation of D-glutamate residue of lipid II, converting it to an isoglutamine residue.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + H2O + L-glutamine = ADP + beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:57928"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:60033"/>
            <dbReference type="ChEBI" id="CHEBI:62233"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate = ADP + beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-O-P-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate</text>
            <dbReference type="Rhea" id="RHEA:59488"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:60033"/>
            <dbReference type="ChEBI" id="CHEBI:143132"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-O-P-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + NH4(+) = beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:57932"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:62233"/>
            <dbReference type="ChEBI" id="CHEBI:143132"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms a heterodimer with GatD.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MurCDEF family. MurT subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.13" evidence="1"/>
    <dbReference type="EMBL" id="CBAR010000040">
        <property type="protein sequence ID" value="CCZ83043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00836"/>
    <dbReference type="EnsemblBacteria" id="CCZ83043">
        <property type="protein sequence ID" value="CCZ83043"/>
        <property type="gene ID" value="BN566_00836"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140282">
        <property type="term" value="F:carbon-nitrogen ligase activity on lipid II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004326">
        <property type="term" value="F:tetrahydrofolylpolyglutamate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02214">
        <property type="entry name" value="Lipid_II_synth_MurT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018109">
        <property type="entry name" value="Folylpolyglutamate_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043703">
        <property type="entry name" value="Lipid_II_synth_MurT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013564">
        <property type="entry name" value="MurT_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08353">
        <property type="entry name" value="MurT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01011">
        <property type="entry name" value="FOLYLPOLYGLU_SYNT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0133">Cell shape</keyword>
    <keyword evidence="1" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Mur_ligase_M" evidence="2">
        <location>
            <begin position="56"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="MurT_C" evidence="3">
        <location>
            <begin position="326"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="453" mass="50641" checksum="6B16866335EAF4EB" modified="2013-07-24" version="1">MKKRISYYVAALVGKAMIHGMHLMKRNATNLPGEVALQLCPSLLANFQMPKHVIVVTGTNGKTTVTNLIGNVLKKCGNDIITNAFGGNVDTGIASLLLANSKLNGTLTADTLLLEMDERSAQRILPYVKPDYLVCTNLQRDSMKRNAHVEFIFDFLNRNIPEKTVVISNADDLVSSQLVPENKHIYFGVALLDGEVPTNDSLVSDIVNCPNCGTRLQFSFRHYNHIGQATCPNCGFCNAKADFQVKKVENCTATIQHHDTTEIYPIPNDRITDIYNSAAVITFLRTYGISQQQVADCLKGSEIVKSRYDTLTSHGKPVYITLAKGQNPIACSAACDFVRKELGKKAVILILEDFYDRRRTSENIAWIYETDFEFLKQDDVVQIIVGGKRATDYLVRLLIAGIDRSRIVCCASETETVQHLQWDKFDKLMILYDLYNTESLSQIKKQLQEDAAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXX9</accession>
    <name>R5UXX9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dockerin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00961</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000069">
        <property type="protein sequence ID" value="CCZ83178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83178">
        <property type="protein sequence ID" value="CCZ83178"/>
        <property type="gene ID" value="BN566_00961"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003343">
        <property type="entry name" value="Big_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002105">
        <property type="entry name" value="Dockerin_1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040753">
        <property type="entry name" value="DUF5620"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008964">
        <property type="entry name" value="Invasin/intimin_cell_adhesion"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18522">
        <property type="entry name" value="DUF5620"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00635">
        <property type="entry name" value="BID_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49373">
        <property type="entry name" value="SSF49373"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00448">
        <property type="entry name" value="CLOS_CELLULOSOME_RPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004406545" description="Dockerin domain-containing protein" evidence="2">
        <location>
            <begin position="33"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="3">
        <location>
            <begin position="801"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="877" mass="95896" checksum="D12B2B0E7317C715" modified="2013-07-24" version="1">MVHTVWKKRAFAAVMSVAMLGTSMAATPIVWAAGTTTEIQVGQELAISSSESESYTDGGGNKQTRPVNWKECRYTSYLKDDAYKNYSSSNKITSLQFNFTAKEQIKKFSYWFGINVTSAYGDWYSPAEEAIEGYIDYGTSESSDFTITIDLSKVEVNYYQLENWAVGQWWNEEKRFDMQNCYAETVSGKEVPVTLKSVTVNGTAKDMMVSTDPNPSNPDNPDNPDKEAQFPNTGEGGFYSSKNEQSSNYSFVDNKDGTATVKSTITKKLNADDLKKLAQKEYGTDTITLTPSPKGADYSEDGYSDKYGDENSEATIRKNGDPLNSHKFSYDDFGIGSGDETTKITPEALTVVMRAKDNQNVTRIMYGGGLNVEAGTPADTEKAKAKIGRDEAGNVTDDSLAGFKKDGNAGYWYNDIGADSYDDVAKNLENVGYKVGSPDTENGLVQIQNGKDLKEQSFGDYVQVTWDVPEDVKQYADAASGKTISFQLWYGEIEAEEYTPLESLDIDSAMLTYTEELTFPYKSQTKELTINKELKTGDDPLEILYSDLGMTYETTADVYAIEFTLDSKVDARQLVLGNGTTVVDKASDYWYQAGSIALVKSDDKNHEYTYMWIMPADVANVTLGEDGTIMSLLNKVSTEKEGDHFSLALYYADNNDGKEVTSVNLKSVSAYYDTDNRKNTAKADLFEKDLSVSPKNITMKVGETKKISTNVDGCTFTSEDPTIATVDENGNVVGVGAGTVVINVETPKGQKDTVTVTVNPADTTATTTTTTTTTTTTTKATTTTTTTQATTTTTTTTTKAFKPYYGDVNLDGVVDLRDTIKLNKYLAGQVTLSDAAMINADVTETSGAVDDKDATKLMRFVLFLVTDLGPGTPDSAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXY6</accession>
    <name>R5UXY6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative cellulose-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00730</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000029">
        <property type="protein sequence ID" value="CCZ82930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82930">
        <property type="protein sequence ID" value="CCZ82930"/>
        <property type="gene ID" value="BN566_00730"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004394606" evidence="1">
        <location>
            <begin position="34"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="531" mass="57572" checksum="C46589AE5CA2DB0E" modified="2013-07-24" version="1">MFFKKNKMAAVALCGMFAAALATPFSAGISASAEEDVTTYAEQVAEADGVFDNLPVAGASANLSPFQVTLQPERTFVSAEELAQGDVHINTKVYFHGDNSISPRAFLLSYHASDTEHVYFDNSLDIRTFGDAKDCTYSGGSYTSRYPEFCLGSTVINSKKQNVFAPFGSCVLGDTIYERMNGGNKIYSDGNGGAYFTLSCNKLVDGVKTFVQEKVEIPASQMQIHADGSASFDYLYYDQNDPNFTQKTATIELPHYDATLSADETYKGFHNTCYWTPAATETPAFFGYTDEFPCFSFDIVLRQGTPAGSYDVQLNDNCRLATSSFLVPLQLMNTTITVGTASGTITQDSTSPYYCFFAENDKEIRLNQGSGELISTVTSADGTTATQDVTDAVTCGQSPAELYAQQSGSAYVNPVPFTLNTQPLKNTDGTDYTKTLLIGKKGDTNLDGKVDVEDAVLVLQYYAKKAAGLESTLLEQATNEEEILSYFLSDIDTCSQNQGTDGGELNVEDAVQILTYYAQAAAGNNPVWSNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>R5UY11</accession>
    <name>R5UY11_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_00996</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000073">
        <property type="protein sequence ID" value="CCZ83213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00996"/>
    <dbReference type="EnsemblBacteria" id="CCZ83213">
        <property type="protein sequence ID" value="CCZ83213"/>
        <property type="gene ID" value="BN566_00996"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008643">
        <property type="term" value="P:carbohydrate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03301">
        <property type="entry name" value="ABC_MalK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015855">
        <property type="entry name" value="ABC_transpr_MalK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040582">
        <property type="entry name" value="OB_MalK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17912">
        <property type="entry name" value="OB_MalK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="SSF50331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="4"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ83213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="364" mass="40918" checksum="49BC098A4894E9AA" modified="2013-07-24" version="1">MSFIEMKNICKKYAGAEKNSVTDFNLSIEEGEFIAFVGPSGCGKSTTLRMIAGFEEITSGDFYIDGKRMNTVLPRDRGISMVFQNYALYPHMTVEKNISYGLKNMKVPADEIKKKVDWAIEILGLSEYRYRKPKNLSGGQRQRVALGRAIVKNQKLFLMDEPLSNLDAKLRVSMRSEISKLHRQLGSTTIYVTHDQVEAMTMADRIVIMKDGVIQQVGKPMDLYEHPANKFVAGFIGSPQMNFYAVKVTGTTMTFSDGNKMHLPESMVSKLNGRQGAFILGIRGEDIKLDPQNLDLYKNNIMSATVDSVEVMGNENNLYFNFGGTGSVARVSKYEISKPGDTIDFTFVPSRMHFFDTQTEESLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5UY75</accession>
    <name>R5UY75_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">HesA/MoeB/ThiF family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_01059</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="3">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01059"/>
    <dbReference type="EnsemblBacteria" id="CCZ83278">
        <property type="protein sequence ID" value="CCZ83278"/>
        <property type="gene ID" value="BN566_01059"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008641">
        <property type="term" value="F:ubiquitin-like modifier activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012731">
        <property type="entry name" value="Adenyl_ThiF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="PTHR10953"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="SSF69572"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02356">
        <property type="entry name" value="adenyl_thiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="ThiF" evidence="5">
        <location>
            <begin position="11"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl persulfide ester intermediate" evidence="1">
        <location>
            <position position="187"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl cysteine dithioester (Cys-Gly) (interchain with G-Cter in ThiS)" evidence="4">
        <location>
            <position position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR612731-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR612731-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR612731-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR612731-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ83278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="270" mass="29253" checksum="A07047AED57B8286" modified="2013-07-24" version="1">MAFTNEQLERYSRHIILKEIGVKGQKKLLNAKVLIIGAGGLGAPVAMYLAAAGVGTIGIADADEVDLSNLQRQIIHATKDVGKPKVESAKETMEAMNPDVKVITYHTFVTSENIMELIQDYDFIIDGTDNFPAKFLINDACVMAKKPFSHAGIIRFKGQLMTYVPGEGPCYRCVFKNPPPKDAVPTCKQAGVVGAMGGVIGSLQAMEAIKYIVGVGDLLTGYLLTYDALTMDFRKVKLPHNTDNCPVCGKHPTITKLIDYEQAECDGVGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYE4</accession>
    <name>R5UYE4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">VTC domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00125</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000090">
        <property type="protein sequence ID" value="CCZ83343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00125"/>
    <dbReference type="EnsemblBacteria" id="CCZ83343">
        <property type="protein sequence ID" value="CCZ83343"/>
        <property type="gene ID" value="BN566_00125"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.100.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018966">
        <property type="entry name" value="VTC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042267">
        <property type="entry name" value="VTC_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09359">
        <property type="entry name" value="VTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VTC" evidence="1">
        <location>
            <begin position="7"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="252" mass="29565" checksum="56CA7D6E42B73E6F" modified="2013-07-24" version="1">MAIETFQRQEIKFLLNEVQYQALTEQLEQYMNPDPYCVGGKDYGIYNIYYDTPDDYLIRTSLAKPYYKEKIRLRSYMSPAKPEDLVFLEIKKKIGGIVNKRRITLTLKEAEAYLQRGIHPVDNGKYVRRLVLEELDHFCDRYAPLVPKQYISYQRAAFFGKDDPNFRLTFDRQLTQRRTNLNLSSGDFGSLLIPENSHLMEVKIGGAMPIWLAQQLAALRIYKISFSKYGTAYRLYCTAEQQTKEKRNVSYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYN8</accession>
    <name>R5UYN8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00937</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000064">
        <property type="protein sequence ID" value="CCZ83150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00937"/>
    <dbReference type="EnsemblBacteria" id="CCZ83150">
        <property type="protein sequence ID" value="CCZ83150"/>
        <property type="gene ID" value="BN566_00937"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="320" mass="37496" checksum="8BF465E64623FD1C" modified="2013-07-24" version="1">MPARKQPEDVPVYLFTGFLEGGKTKFIQETMEDPRFNKGERTLLLLCEEGEEEYDISTYPAKNVYQEVIEDESELNPEHLTELLEKHQAERVVLEYNGMWQLKFLFEHIPDNWTVYQEMCFVDATTFMNYNANMRSLVVDKLNTCELVVFNRFTKDMDKMEFHKIVRAVSRRTDIAYEYTDGKVEYDQIEDPLPFDINAPVIQIDDRDYAIWYRDLMEDMNKYEGKTVKFRGIVAVDKKFPKGTFAVGRHVMTCCVEDITYQCVVAEWSNAFALSNRQWVNITASIHITRHKLYRGKGPVLTVKEVAVTSKPEQEVATFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYQ2</accession>
    <name>R5UYQ2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00948</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000069">
        <property type="protein sequence ID" value="CCZ83165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83165">
        <property type="protein sequence ID" value="CCZ83165"/>
        <property type="gene ID" value="BN566_00948"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="120" mass="13468" checksum="AB27C3A6F59C777C" modified="2013-07-24" version="1">MAQDTENRIDGQIESASKPQRWNKRLIAIGVIGSSLLIGIPIIGYGIKRRTKSLLEFFRIFRENPVWTMGFLGILILITGILYILARKITRKPHTHLLEGCIILAGLLMGIGFIAIQELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYR9</accession>
    <name>R5UYR9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Transcription elongation factor GreA</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Transcript cleavage factor GreA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">greA</name>
        <name evidence="9" type="ORF">BN566_00968</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus. GreA releases sequences of 2 to 3 nucleotides.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the GreA/GreB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000069">
        <property type="protein sequence ID" value="CCZ83185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00968"/>
    <dbReference type="EnsemblBacteria" id="CCZ83185">
        <property type="protein sequence ID" value="CCZ83185"/>
        <property type="gene ID" value="BN566_00968"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070063">
        <property type="term" value="F:RNA polymerase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032784">
        <property type="term" value="P:regulation of DNA-templated transcription, elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00105">
        <property type="entry name" value="GreA_GreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036953">
        <property type="entry name" value="GreA/GreB_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006359">
        <property type="entry name" value="Tscrpt_elong_fac_GreA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028624">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001437">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023459">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022691">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036805">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30437">
        <property type="entry name" value="PTHR30437"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01272">
        <property type="entry name" value="GreA_GreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03449">
        <property type="entry name" value="GreA_GreB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006092">
        <property type="entry name" value="GreA_GreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46557">
        <property type="entry name" value="SSF46557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01462">
        <property type="entry name" value="greA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="1 5 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="GreA_GreB_N" evidence="8">
        <location>
            <begin position="5"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="GreA_GreB" evidence="7">
        <location>
            <begin position="83"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ83185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="156" mass="17304" checksum="4E31732C8B843E13" modified="2013-07-24" version="1">MAMKQISKSGYDKLKEELDYLVGVKRAEMAQKLKEARAQGDLSENAEYDEAKNEQAILEAQIKEIQYTLDNCEVVDDDSISLDEVGLGSTIKLKNFQSGKVETWQIVSSNEANFAEHKISDDSPIGKAALKKKVGETFQVFAPVGTLLFEVLEIGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZ05</accession>
    <name>R5UZ05_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Endoribonuclease L-PSP</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01292</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RutC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000121">
        <property type="protein sequence ID" value="CCZ83523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01292"/>
    <dbReference type="EnsemblBacteria" id="CCZ83523">
        <property type="protein sequence ID" value="CCZ83523"/>
        <property type="gene ID" value="BN566_01292"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006056">
        <property type="entry name" value="RidA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035959">
        <property type="entry name" value="RutC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006175">
        <property type="entry name" value="YjgF/YER057c/UK114"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11803">
        <property type="entry name" value="PTHR11803"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01042">
        <property type="entry name" value="Ribonuc_L-PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55298">
        <property type="entry name" value="SSF55298"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00004">
        <property type="entry name" value="TIGR00004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="126" mass="13255" checksum="5AE8875C7CDD4A91" modified="2013-07-24" version="1">METIYTKTAPDAIGPYSQAVKIGNTLYSSGQIAINPATGKVEATDIEGQSRQVMQNLAEVLKAAGASFDNVVKTTCFLKDVAKDFAAFNAIYAEAMGDNKPARSCVGVAELPKGVLVEVEVIAVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZ22</accession>
    <name>R5UZ22_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator Fur family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01321</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Fur family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000130">
        <property type="protein sequence ID" value="CCZ83553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01321"/>
    <dbReference type="EnsemblBacteria" id="CCZ83553">
        <property type="protein sequence ID" value="CCZ83553"/>
        <property type="gene ID" value="BN566_01321"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07153">
        <property type="entry name" value="Fur_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002481">
        <property type="entry name" value="FUR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043135">
        <property type="entry name" value="Fur_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33202">
        <property type="entry name" value="PTHR33202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01475">
        <property type="entry name" value="FUR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="139" mass="15906" checksum="770B4FEE5C6E01BB" modified="2013-07-24" version="1">MGYHTKQKERILDLLKTCAGEHLTVSDLGKQLEENGTPVGTATIYRYLEQLTEEGVLRKYILDGKSSACYQYLGEHATCQEHFHLKCLSCGTLFHVQCTFLDEMQAHIAAHHHFQIDHTKTVLYGYCEQCQNAQQKKES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZ28</accession>
    <name>R5UZ28_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01086</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83305">
        <property type="protein sequence ID" value="CCZ83305"/>
        <property type="gene ID" value="BN566_01086"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="127" mass="14752" checksum="4928A9214D317724" modified="2013-07-24" version="1">MTTLFGIAFLLAILALIEGICICTKRKLHNQKRPASCYVLLSALELRPDWKAELNHLFSDLQWQVSLQTAEIFLLLPEEPTLQSEEIRQYCQDHTRFQCGTFSEFQKILMECRFPSKNDCNLSEKIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZ38</accession>
    <name>R5UZ38_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TrkA-N domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01346</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000132">
        <property type="protein sequence ID" value="CCZ83578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01346"/>
    <dbReference type="EnsemblBacteria" id="CCZ83578">
        <property type="protein sequence ID" value="CCZ83578"/>
        <property type="gene ID" value="BN566_01346"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006037">
        <property type="entry name" value="RCK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036721">
        <property type="entry name" value="RCK_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02080">
        <property type="entry name" value="TrkA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116726">
        <property type="entry name" value="SSF116726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51202">
        <property type="entry name" value="RCK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RCK N-terminal" evidence="1">
        <location>
            <begin position="12"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="RCK C-terminal" evidence="2">
        <location>
            <begin position="142"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="226" mass="25052" checksum="088E5616FA170961" modified="2013-07-24" version="1">MFGKKVKTENGLYGIIGLGRFGYALAQTLSESGKDLIIIDENEAKINSAAAFTDHAYVVQELSKENFRKVGIDNCDVVIICIGERIDTCILATLNVIQLGVKRVIAKATSAEQGCVLELLGAEVVYPERDMAIRLANRLLAPGFLDFITLSEEVDLMELSVTQKIAEKTVQEINPRKMFGINLIAVKHNGSLEIEIKPDTILHRGDTVCVIGTRENIRKFERFLNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZ93</accession>
    <name>R5UZ93_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01131</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000092">
        <property type="protein sequence ID" value="CCZ83355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83355">
        <property type="protein sequence ID" value="CCZ83355"/>
        <property type="gene ID" value="BN566_01131"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="84" mass="9788" checksum="4A18D823604DC8B0" modified="2013-07-24" version="1">MDNETEELVNRALYKQIKSMNRAEMETFVRNVFAQGYQRAEEETHPIDYDSLRADLSKIKGIGESRLNEIMTVIDKHIAINNDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZB2</accession>
    <name>R5UZB2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01414</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000148">
        <property type="protein sequence ID" value="CCZ83678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01414"/>
    <dbReference type="EnsemblBacteria" id="CCZ83678">
        <property type="protein sequence ID" value="CCZ83678"/>
        <property type="gene ID" value="BN566_01414"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010064">
        <property type="entry name" value="HK97-gp10_tail"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04883">
        <property type="entry name" value="HK97-gp10_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="127" mass="14331" checksum="04236909B46684C9" modified="2013-07-24" version="1">MIKTIRVDKLADEIMKELQEYSNATSDDVKAAVKKSSQAVKKELLQTAPKRTGTYRKSFVVTKIEENSSKLKVAVHSKKHYRLSHLLEDGHALRQGGRTNAHPHMKPAEEHGIEMLESLVKKSLGRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZD0</accession>
    <name>R5UZD0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ricin-type beta-trefoil lectin domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01434</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000154">
        <property type="protein sequence ID" value="CCZ83698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01434"/>
    <dbReference type="EnsemblBacteria" id="CCZ83698">
        <property type="protein sequence ID" value="CCZ83698"/>
        <property type="gene ID" value="BN566_01434"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026444">
        <property type="entry name" value="Secre_tail"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04183">
        <property type="entry name" value="Por_Secre_tail"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0430">Lectin</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="280" mass="29734" checksum="446356AC7FC53331" modified="2013-07-24" version="1">MVTGKTATPNAKTYNVRNLDAYVSFGDLKAGTYYYRVFATNATTTNFPVVEQKFTVSANGSTTASDTLSISGGTSVPNITEGTSVVVKGTVSSASSNITSVTVGVYSASGNLITGKTAKPNAKSYDLRKLDAYVNFNLLTPGSYLYRVTVSNGTKTQQLVNQAFQVKAKGGSSQSQDRLTLSGGRTIPNIKVGKAVSIRGTVKSATSNITSLTVGVFTASGNFVTGKTVKPNAKSYDVSRLDNYVNFNILSADTYYYGIIATNGSYQNQIVSYQKFKVTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZD8</accession>
    <name>R5UZD8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">Amidophosphoribosyltransferase</fullName>
            <shortName evidence="5">ATase</shortName>
            <ecNumber evidence="3 5">2.4.2.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Glutamine phosphoribosylpyrophosphate amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN566_01444</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = 5-phospho-alpha-D-ribose 1-diphosphate + H2O + L-glutamine</text>
            <dbReference type="Rhea" id="RHEA:14905"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="EC" id="2.4.2.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="6">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="7">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 5">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.14" evidence="3 5"/>
    <dbReference type="EMBL" id="CBAR010000154">
        <property type="protein sequence ID" value="CCZ83708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01444"/>
    <dbReference type="EnsemblBacteria" id="CCZ83708">
        <property type="protein sequence ID" value="CCZ83708"/>
        <property type="gene ID" value="BN566_01444"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00124"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004044">
        <property type="term" value="F:amidophosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005854">
        <property type="entry name" value="PurF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000485">
        <property type="entry name" value="Amd_phspho_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 5" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="8">
        <location>
            <begin position="1"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="349"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="350"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="386"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="465"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="468"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ83708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="473" mass="53012" checksum="FA35EF3C6F6607A6" modified="2013-07-24" version="1">MGGFFGAVSANDCISDVFFGTDYHSHLGTRRGGMTAFSPALGFQRNIHSIENSPFRTKFEKDVERMRGNICIGCISDTDPQPIMVRSQLGLYAICSIGVITNAETLSEELLDKGCANFETMSSGSINSGALIAALIAQRSSFVEGIRYAQEKIQGTMSLVIMTEDSIICARDKMGRLPILIGKRSDGFCFSFESFAYQKLGYQTCHELKAGEILKLTKDGYEILQEGTDEMKICTFLWTYYGYPNACYEGVNVEMMRTRNGEIMAETDIKNGKLPDVDYVCGVPDSGVPHAIGYANRSGIPFARPFIKYTPTWPRSFMPTSQTMRNKVAKMKLIPVHDLIEGKKLLFVDDSIVRGTQMRETVEFLYENGAKEVHMRSACPPIMYGCKYLNFSRSTSELELIARQIIDEHEGIDGIKYIHEYSNSNTERGKLLRDEICRRLKLTSLEFQSLEGTVQAVGKPECQLCSYCWSGRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZK4</accession>
    <name>R5UZK4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transcription antitermination protein NusB</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Antitermination factor NusB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">nusB</name>
        <name evidence="8" type="ORF">BN566_01219</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in transcription antitermination. Required for transcription of ribosomal RNA (rRNA) genes. Binds specifically to the boxA antiterminator sequence of the ribosomal RNA (rrn) operons.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the NusB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000105">
        <property type="protein sequence ID" value="CCZ83445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01219"/>
    <dbReference type="EnsemblBacteria" id="CCZ83445">
        <property type="protein sequence ID" value="CCZ83445"/>
        <property type="gene ID" value="BN566_01219"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription, termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031564">
        <property type="term" value="P:transcription antitermination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.940.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00073">
        <property type="entry name" value="NusB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035926">
        <property type="entry name" value="NusB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011605">
        <property type="entry name" value="NusB_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006027">
        <property type="entry name" value="NusB_RsmB_TIM44"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11078">
        <property type="entry name" value="PTHR11078"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01029">
        <property type="entry name" value="NusB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48013">
        <property type="entry name" value="SSF48013"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01951">
        <property type="entry name" value="nusB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 6" id="KW-0889">Transcription antitermination</keyword>
    <keyword evidence="4 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NusB" evidence="7">
        <location>
            <begin position="10"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="145" mass="16440" checksum="860619D5FEE88024" modified="2013-07-24" version="1">MAQHEMTRHETRECAFYILFEMQFQNDTAEELFQVAEEADLLPITDAVKEMVNGVVAHEAELDEIISAYSTKRVINRIPKINLSILRLALYEIRYDAQMPMNAAIAEAISLAKAYGYQEDISFINGVLGNYARDHAKDTGTEQHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZL6</accession>
    <name>R5UZL6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Toxin-antitoxin system antitoxin component Xre family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01533</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000164">
        <property type="protein sequence ID" value="CCZ83798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83798">
        <property type="protein sequence ID" value="CCZ83798"/>
        <property type="gene ID" value="BN566_01533"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="7"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="116" mass="13810" checksum="9714779E6489F4FA" modified="2013-07-24" version="1">MQHYQRIRDLREDSDLTQEALCKKLYMHKTTYTNYEQGKHTVPLDFAVQLADFYQVSIDYIAGRTNFPQGMPVVAEETDCPKYFSDLTERNKGRVEQFVEQLYEQQQTEFQQKEAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZM9</accession>
    <name>R5UZM9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Chaperone protein DnaK</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">HSP70</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Heat shock 70 kDa protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Heat shock protein 70</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">dnaK</name>
        <name evidence="12" type="ORF">BN566_01239</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">Acts as a chaperone.</text>
    </comment>
    <comment type="induction">
        <text evidence="9">By stress conditions e.g. heat shock.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9 10">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000111">
        <property type="protein sequence ID" value="CCZ83465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01239"/>
    <dbReference type="EnsemblBacteria" id="CCZ83465">
        <property type="protein sequence ID" value="CCZ83465"/>
        <property type="gene ID" value="BN566_01239"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00332">
        <property type="entry name" value="DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012725">
        <property type="entry name" value="Chaperone_DnaK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="PTHR19375"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="SSF100920"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="SSF100934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02350">
        <property type="entry name" value="prok_dnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0346">Stress response</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="578"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="600"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphothreonine; by autocatalysis" evidence="9">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CCZ83465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="616" mass="65722" checksum="69EA49327DE876C5" modified="2013-07-24" version="1">MGKIIGIDLGTTNSCVAVMEGGNAVVIPNAEGARTTPSVVGFTKNGERLVGQVAKRQAVANPERTISSIKRHMGSDYKVEIDGKKYTPQEISAMILQKLKADAEAYLGAPVTEAVITVPAYFTDAQRQATKDAGQIAGLTVKRIINEPTAAALSYGIDKEDDQKVMVYDLGGGTFDVSIIEMGDGVQQVLATAGNNHLGGDDFDQRIIDWIVSEFKKTEGIDLSTDKMVMQRLKEAAEKAKIELSATTSSNINLPFITADATGPKHLDMTLTVAKFNELTKDLVEATMGPVKQALSDSGLSSSELNKVLMVGGSSRIPAVQEAVRRMLGKEPFKGINPDECVALGAAYQGGVLGGDVKDGLLLLDVTPLSLGLETMGGVCTKIIERNTTIPTKKSQIFSTAADNQTAVDINVLQGEREFAKDNKQLGTFRLDGIAPARRGVPQIEVTFDIDANGIVHVSAKDLGTGKEQNITITSSTNMSKDDIDKAVKEAEAYAEEDKKRKDEVDTKNNAENMVMQCESTLEELGDKVPASEKSDVESKCNDLKEALKGTDTALIKQKSEDLQKALYDLSSKLYQQAGAAQGNPDMGGAATGEADNSGKNNGGDDDVIDADYKEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZP8</accession>
    <name>R5UZP8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Stress responsive A/B Barrel Domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01563</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000165">
        <property type="protein sequence ID" value="CCZ83828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83828">
        <property type="protein sequence ID" value="CCZ83828"/>
        <property type="gene ID" value="BN566_01563"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013097">
        <property type="entry name" value="Dabb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07876">
        <property type="entry name" value="Dabb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00886">
        <property type="entry name" value="Dabb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51502">
        <property type="entry name" value="S_R_A_B_BARREL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Stress-response A/B barrel" evidence="1">
        <location>
            <begin position="2"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="101" mass="11494" checksum="73BDE5BA4FBA05BE" modified="2013-07-24" version="1">MMKHIVMFKFQAEANGKTAMENAAAAKEKAEQLPAQIPQLKKLEVRLNSPSADPTNYEFALICDFETMDDLNTYQVHPAHVAFGQFIKPLREARACIDYEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZQ9</accession>
    <name>R5UZQ9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Aldo_ket_red domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01573</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the shaker potassium channel beta subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000166">
        <property type="protein sequence ID" value="CCZ83838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01573"/>
    <dbReference type="EnsemblBacteria" id="CCZ83838">
        <property type="protein sequence ID" value="CCZ83838"/>
        <property type="gene ID" value="BN566_01573"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005399">
        <property type="entry name" value="K_chnl_volt-dep_bsu_KCNAB-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NADP_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43150">
        <property type="entry name" value="PTHR43150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="SSF51430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldo_ket_red" evidence="2">
        <location>
            <begin position="27"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="331" mass="36823" checksum="37757ADC2687E819" modified="2013-07-24" version="1">MYVPNTERYAAMAYRRCGKSGLKLPEVSLGLWHNFGDTNTLEHMEAICKAAFDGGITHFDLANNYGPPAGAAEQNFGKILKTLFAGYRDELIISTKAGFDMWEGPYGNWGSRKYLLASLDQSLKRMGLEYVDIFYHHRPDPETPLEETMGALAQAVQSGKALYVGLSNYDGATMQQACAILKELHCPFVINQNCYSMLNRTIEQNGLLDACAEEQKGLIVFSPLAQGLLTDRYLHGIPADSRMRTDGRFLNEKQLTPERLQLLQKLQAAAQARGEKLAQLAMHWILRQPAVTSVLIGASKPEQVQDALEMVQQAPLTDADIQEIEQILKAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5V087</accession>
    <name>R5V087_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phage terminase small subunit P27 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01406</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000148">
        <property type="protein sequence ID" value="CCZ83670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01406"/>
    <dbReference type="EnsemblBacteria" id="CCZ83670">
        <property type="protein sequence ID" value="CCZ83670"/>
        <property type="gene ID" value="BN566_01406"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="117" mass="13298" checksum="E17B61C968AB1663" modified="2013-07-24" version="1">MTKSKWKSQIKKACIAINTYKESFDGVIDSLADILEKRDQTLETYDGNPIIEHTNSHGETNRTKNPSLMLWDELNKTALAYWRDLGLTPKGLKNIDEQAMKKKKTDTLAEVLKSLGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0C8</accession>
    <name>R5V0C8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">3-dehydroquinate dehydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01744</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000180">
        <property type="protein sequence ID" value="CCZ84018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01744"/>
    <dbReference type="EnsemblBacteria" id="CCZ84018">
        <property type="protein sequence ID" value="CCZ84018"/>
        <property type="gene ID" value="BN566_01744"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014937">
        <property type="entry name" value="DUF1810"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036287">
        <property type="entry name" value="Rv1873-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08837">
        <property type="entry name" value="DUF1810"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140736">
        <property type="entry name" value="SSF140736"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos" evidence="2">
        <location>
            <begin position="178"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="479" mass="54861" checksum="23650126C062DD19" modified="2013-07-24" version="1">MKYDLSKFVLAHRSYYSTALSEMKNGRKASHWMWYIFPQLKGLGKSSTSSYYGITDLEEAKDFLNDAYLGSNLLEISSALLEIECNDASKVMGKPDDLKLKSSMTLFALASSKESVFQQVLDKYFGGKMDHRTLKLLGMPFEGHKENSPKKYEERIIQIPDDNIQSIRNRYPGGLHFVVGDTHGESTTLRLLMDKIQFNPQIDHVYFLGDYNCGGNVYALLHDIAKYYQEDYAVPGFHLIRGNHERELYPIFELKNMPDIIVIRGTVMNYYLAHAGMVDKAFDLINKDIESTYKPVYAYALDETVAGYDAPLRQVVWSRNGLYSQRSHYHVWPSEKSLQAANACIIHGHTPFSQLKRGNFFSYGDRMLFWENQKVWFAEDLQSFDIDSNVKGRFDTSDLYRGLSCVCLEMIDEIAAKGNGVLKREVLRESENFVFSVPHTPIENTNVDTDLSNLLQAKPAMKRILLDNDGMLQLRDDHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0L9</accession>
    <name>R5V0L9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Stage IV sporulation protein A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01863</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000192">
        <property type="protein sequence ID" value="CCZ84138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01863"/>
    <dbReference type="EnsemblBacteria" id="CCZ84138">
        <property type="protein sequence ID" value="CCZ84138"/>
        <property type="gene ID" value="BN566_01863"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043934">
        <property type="term" value="P:sporulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014201">
        <property type="entry name" value="Spore_IV_A"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09547">
        <property type="entry name" value="Spore_IV_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="78" mass="8770" checksum="480601C7F66E6AC1" modified="2013-07-24" version="1">MAKDIYEDIAKRTGGDIYIGVVGPVRTGKSTLIKQLMEKLVIPNIPEESKRSFPIFQKNPNACEQLTNCRNLLPEKPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0R7</accession>
    <name>R5V0R7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cysteine desulfurase</fullName>
            <ecNumber evidence="4">2.8.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN566_01918</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[sulfur carrier]-H + L-cysteine = [sulfur carrier]-SH + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:43892"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="EC" id="2.8.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 8">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.7" evidence="4"/>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01918"/>
    <dbReference type="EnsemblBacteria" id="CCZ84193">
        <property type="protein sequence ID" value="CCZ84193"/>
        <property type="gene ID" value="BN566_01918"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031071">
        <property type="term" value="F:cysteine desulfurase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044571">
        <property type="term" value="P:[2Fe-2S] cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020578">
        <property type="entry name" value="Aminotrans_V_PyrdxlP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010240">
        <property type="entry name" value="Cys_deSase_IscS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016454">
        <property type="entry name" value="Cysteine_dSase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11601:SF34">
        <property type="entry name" value="PTHR11601:SF34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005572">
        <property type="entry name" value="NifS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00595">
        <property type="entry name" value="AA_TRANSFER_CLASS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotran_5" evidence="9">
        <location>
            <begin position="2"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ84193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="393" mass="42450" checksum="7BAD53B46AE19A25" modified="2013-07-24" version="1">MIYADHAATTKMSAEAMAAMVKGWEVYGNPSSLHTIGQEAAVVLQKAREQIAVCLDADPKEIYFTSGGSEADNQALRSAAAIGKRKGHMHIISTAFEHHAILHTLEQLKAEGFSVTLLDVHENGMITPEQVEAAIQPDTCLVTVMYANNEIGSILPIAEIGAVCRKHHVLFHTDAVQAVGHIPISVKNDNIDMLSLSAHKFHGPKGIGVLYAKKGVRLTSLIAGGAQERGKRAGTENIPAILGMAAALQDACDHMEENTRHCLELRERLIAGLSKIPHSVLNGDREHRLPSNVHFCFEGIEGESLLLLLDQKGICASSGSACTSGSLDPSHVLLAIGRPHEIAHGSLRLSLGEENTMAEVDYMLQAIPEVVAYLRHMSPVWRDLESGKKSFLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0V1</accession>
    <name>R5V0V1_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">zinc_ribbon_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01953</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84228">
        <property type="protein sequence ID" value="CCZ84228"/>
        <property type="gene ID" value="BN566_01953"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026870">
        <property type="entry name" value="Zinc_ribbon_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13240">
        <property type="entry name" value="zinc_ribbon_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004394552" description="zinc_ribbon_2 domain-containing protein" evidence="2">
        <location>
            <begin position="24"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="zinc_ribbon_2" evidence="3">
        <location>
            <begin position="2"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="231" mass="26028" checksum="CBA049AE355E99B0" modified="2013-07-24" version="1">MFCKNCGAQISETTRFCPKCGTAATPPPSVTSPNSSFSSQQSSSFSTTAQNTAAKIKKNPIQLVLIVAGILVLLIIVIAFCNRSKNGMKSSSKAAGAYLVAVYDEDVNDILDLVPDEIIKQIMKNYGCSKKQLKEAVKAELRNESEDYPHCDSVTSYGSTKQTRQYSEYIDRRVEDCMDEDKISKMELCVVNVEDDYYYYDQPAYQYGRDKHTWYSMDATNFVAYAVWEKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0V3</accession>
    <name>R5V0V3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative cell division protein FtsL</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01629</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000171">
        <property type="protein sequence ID" value="CCZ83900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01629"/>
    <dbReference type="EnsemblBacteria" id="CCZ83900">
        <property type="protein sequence ID" value="CCZ83900"/>
        <property type="gene ID" value="BN566_01629"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="65"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="157" mass="18008" checksum="1AFEE276A2AFB1FB" modified="2013-07-24" version="1">MKVTDETVPEEDLEEAEDAAVVEKRPRRKLFYIEAGKRGTLAKIMIGSILALVLFSLVINSYVQLNEVYSQISAAESTLNDLQSENVRMQTQLEGQASIRNIKEYAEDRLGLQKLDRSQIQYIQIQTEDEVVVEEPDQNIFVQIKRKFNDLIAYLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5V132</accession>
    <name>R5V132_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA-binding helix-turn-helix protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00216</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000210">
        <property type="protein sequence ID" value="CCZ84303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84303">
        <property type="protein sequence ID" value="CCZ84303"/>
        <property type="gene ID" value="BN566_00216"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13443">
        <property type="entry name" value="HTH_26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="11"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="81" mass="9532" checksum="85BC8BEC6538B7EC" modified="2013-07-24" version="1">MTLQDCVVQRIYRLLIQMDWTMKQLSEQCGIPLAELESLMKQETADWTLEQLCTICRAFSISLPEFFDAPMFQRLFLPETP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5V137</accession>
    <name>R5V137_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Riboflavin synthase alpha subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01704</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000178">
        <property type="protein sequence ID" value="CCZ83975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01704"/>
    <dbReference type="EnsemblBacteria" id="CCZ83975">
        <property type="protein sequence ID" value="CCZ83975"/>
        <property type="gene ID" value="BN566_01704"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00402">
        <property type="entry name" value="Riboflavin_synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023366">
        <property type="entry name" value="ATP_synth_asu-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001783">
        <property type="entry name" value="Lumazine-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026017">
        <property type="entry name" value="Lumazine-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21098">
        <property type="entry name" value="PTHR21098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00677">
        <property type="entry name" value="Lum_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000498">
        <property type="entry name" value="Riboflavin_syn_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="SSF63380"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00187">
        <property type="entry name" value="ribE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51177">
        <property type="entry name" value="LUMAZINE_BIND"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="Lumazine-binding" evidence="2">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="Lumazine-binding" evidence="3">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="repeat" description="Lumazine-binding" evidence="2">
        <location>
            <begin position="97"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="Lumazine-binding" evidence="3">
        <location>
            <begin position="97"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="210" mass="22120" checksum="53866DB769AB88C1" modified="2013-07-24" version="1">MFTGIIEEMGTVRRLDRTPISCQLTIAATLVLQGTQIGDSIAVNGVCLTVTDLQKDAFTADVMPETLRRSSLGILRSGSSVNLERAMVANGRFGGHIVSGHIDGTGTIASKVPEGNAQIVTISAAPNLLRYIVEKGSIAIDGISLTVASVTKSSFSVSLIPHTGACTTLLSKRPGDPVNLETDVIGKYVEKLMQPTTPSLTASMLQQYGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5V149</accession>
    <name>R5V149_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator PadR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01714</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000178">
        <property type="protein sequence ID" value="CCZ83985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01714"/>
    <dbReference type="EnsemblBacteria" id="CCZ83985">
        <property type="protein sequence ID" value="CCZ83985"/>
        <property type="gene ID" value="BN566_01714"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005149">
        <property type="entry name" value="Tscrpt_reg_PadR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03551">
        <property type="entry name" value="PadR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PadR" evidence="1">
        <location>
            <begin position="16"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="118" mass="13565" checksum="00D84EA700DADE72" modified="2013-07-24" version="1">MTLKINLQKSVAEMLVLSILNNGTMYGYQLIQELEAQSKGLFSIKEGTLYPLLYRLIDNNMVSFEKKLVGKRRTRVYYNITEDGKEYLQQILKSYQDMVQGVANVLVKNGQSIHIVTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5V151</accession>
    <name>R5V151_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00231</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000212">
        <property type="protein sequence ID" value="CCZ84318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00231"/>
    <dbReference type="EnsemblBacteria" id="CCZ84318">
        <property type="protein sequence ID" value="CCZ84318"/>
        <property type="gene ID" value="BN566_00231"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017501">
        <property type="entry name" value="Phage_infect_YhgE_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017500">
        <property type="entry name" value="Phage_infect_YhgE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023908">
        <property type="entry name" value="xxxLxxG_rpt"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03062">
        <property type="entry name" value="pip_yhgE_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03061">
        <property type="entry name" value="pip_yhgE_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03057">
        <property type="entry name" value="xxxLxxG_by_4"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="680"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="720"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="753"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="780"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="833"/>
            <end position="854"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="885" mass="92784" checksum="DDA6B0F48CA008C0" modified="2013-07-24" version="1">MKHAEKEEKQPSFLKRHFNAIATICIVLIPTIYTTLFLGSMWDPYGNVSDLPVAVVNQDQSVTFHDQTLSIGDDLIDNLKDNDSLDFSFVDAKTAQDGLEDGTYYMAITIPEDTSANASTVLDDTPKKVQLYYETNPGTNYIASKMSETAMQKLKTEVSSTITEAYTETLFDQIGTAGDGMQDAADGAKELDDGAQKLKDGSVTLSDNLKVLADSTLTLEEGCGTLTDGLTAYTDGVAQVDDGAKQLEDGMNQLQDGVSPLTDGASQLQSGASQLQSGVDAYTEGVASAYTGAQSLSSGSQQLSAGSQNLQDGAAQLYEGSRQLEAGLSQLSSSLSQSCNEETTAQIKTLSAGLEQLQTGIDTLKSSVSNSGTATTEAMQAVAGDLQTLGSGLASLQSAQQALMSTDAFQSLDAESQQELLGCFAEPMSSLSTAATQLQSDVTALATALEPSAQLPDAVNQLAAGADQALPAANEALASLSGGLQTVQSAVDDQLLPGASALTDGAASLSDGSATLSSGLQTAAAGASALTDGLGTLHSNSSSLQNGAIALDDGISTLVEKMPTLTVAINQLQSGSKTLSDGTGQLTAQNDTLLSGAKQLQDGAAALQDGAVQLADGSATLKDGLVTLTDGSGTLWTALQDGATEINDINSTDDTYDMFASPVDAEETYASHVENNGHAMAAYMMAVGLWVACLAYCIMFSPYEVPLTGKNAMHSWAKKLPVLLMVAWVQAIGMVLLLHLFNGFTPQDMLQTILVAGFSSMAYMAIIFCLNMYCGKIGSFILLVFMVLQLSGCAGTYPSELSAKFFRVIKPFMPFTYTVQGFRSSIATGRSNATTYVVMIALAAVFTLLMIPAYKMKWKREKTQADFTEETETTSIASHKQAVSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1K6</accession>
    <name>R5V1K6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Thiamine biosynthesis protein ThiS</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01875</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000192">
        <property type="protein sequence ID" value="CCZ84150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01875"/>
    <dbReference type="EnsemblBacteria" id="CCZ84150">
        <property type="protein sequence ID" value="CCZ84150"/>
        <property type="gene ID" value="BN566_01875"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016155">
        <property type="entry name" value="Mopterin_synth/thiamin_S_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010035">
        <property type="entry name" value="Thi_S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003749">
        <property type="entry name" value="ThiS/MoaD-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34472">
        <property type="entry name" value="PTHR34472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02597">
        <property type="entry name" value="ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54285">
        <property type="entry name" value="SSF54285"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01683">
        <property type="entry name" value="thiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="70" mass="7873" checksum="EB4DDC72562CCAC2" modified="2013-07-24" version="1">MRVNGKEIPLNAPISLQAFLLEQHYDLRTIAVERNEEIVPKATYAEVMLQDCDVLEVVHFMGGGQSSKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1Q4</accession>
    <name>R5V1Q4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 14">Crossover junction endodeoxyribonuclease RuvC</fullName>
            <ecNumber evidence="11 14">3.1.21.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13 14">Holliday junction nuclease RuvC</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12 14">Holliday junction resolvase RuvC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ruvC</name>
        <name evidence="15" type="ORF">BN566_01940</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 14">Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 14">
            <text>Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).</text>
            <dbReference type="EC" id="3.1.21.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="14">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 14">Belongs to the RuvC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.21.10" evidence="11 14"/>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01940"/>
    <dbReference type="EnsemblBacteria" id="CCZ84215">
        <property type="protein sequence ID" value="CCZ84215"/>
        <property type="gene ID" value="BN566_01940"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008821">
        <property type="term" value="F:crossover junction endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16962">
        <property type="entry name" value="RuvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00034">
        <property type="entry name" value="RuvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020563">
        <property type="entry name" value="X-over_junc_endoDNase_Mg_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002176">
        <property type="entry name" value="X-over_junc_endoDNase_RuvC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30194">
        <property type="entry name" value="PTHR30194"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02075">
        <property type="entry name" value="RuvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00696">
        <property type="entry name" value="RSOLVASERUVC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00228">
        <property type="entry name" value="ruvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01321">
        <property type="entry name" value="RUVC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 14" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 14" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="8 14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 14" id="KW-0540">Nuclease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="14">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CCZ84215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="164" mass="18251" checksum="56D35E663471CB3D" modified="2013-07-24" version="1">MRILGVDPGYAIVGFGVLEYTGTTFTPITYGAITTAAETPFLRRLESIYRDFRTLIQQYHPDCVAMERLYFTSNQKTVIDVAQARGVLMLTAQLEQIPVSEYTPLQVKQAVVGYGRAEKKQVMELTRRILKLQKIPKPDDAADALAIAICHGHCATGLQGRMES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1R5</accession>
    <name>R5V1R5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01955</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84230">
        <property type="protein sequence ID" value="CCZ84230"/>
        <property type="gene ID" value="BN566_01955"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="130" mass="15511" checksum="DBE73B71F6A46C1F" modified="2013-07-24" version="1">MHHQLYGFRFLYFDFHFSAITNFIILEIQNNRFYLQCYHGSDTYTIDITDQIGTFCESMTNINLNDWNRINFDFPMELFPGFYWNLEIETDTISLLCKGLHNFPPNWNDFKTILHTLGIKKGLDASNPFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1Y3</accession>
    <name>R5V1Y3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycoside hydrolase family 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00233</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000213">
        <property type="protein sequence ID" value="CCZ84320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00233"/>
    <dbReference type="EnsemblBacteria" id="CCZ84320">
        <property type="protein sequence ID" value="CCZ84320"/>
        <property type="gene ID" value="BN566_00233"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01831">
        <property type="entry name" value="Endoglucanase_E_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040794">
        <property type="entry name" value="CE2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037461">
        <property type="entry name" value="CtCE2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013830">
        <property type="entry name" value="SGNH_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17996">
        <property type="entry name" value="CE2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13472">
        <property type="entry name" value="Lipase_GDSL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CE2_N" evidence="2">
        <location>
            <begin position="20"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="SGNH_hydro" evidence="1">
        <location>
            <begin position="123"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="336" mass="36092" checksum="F0254941E9E5AE1B" modified="2013-07-24" version="1">MKPIRSAVTCSPGTRYAPETDTLWLALSNATISCTTTATTVTVSLRAIGSVRVAVFLQEKMVMDFVCTDETKIISIPCPSESAQQIQICKLSEPHYGILGVSALTCDGGTCSPLPPAPHHLAFIGDSITCGYGIDAASPEDAFSIATEHAAKSYAAQTAKLLHADAHILSYSGFGVYSGFPEESCKNETDLLPLVYEAAGFYHYQPENWESTILLPFAPPEAVILYLGTNDSAYLHLFPEKGAAFQAAYAAFLQKLRSEFPTAFLVCMLGMSSDLDAAPFIRNVVAAAQQQDNRPALCDVRSIVGTDFGANHHPGAVTQRNAAKLLADLLQKYLPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1Z4</accession>
    <name>R5V1Z4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Arginine/lysine/ornithine decarboxylases</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_00248</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Orn/Lys/Arg decarboxylase class-I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000215">
        <property type="protein sequence ID" value="CCZ84335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00248"/>
    <dbReference type="EnsemblBacteria" id="CCZ84335">
        <property type="protein sequence ID" value="CCZ84335"/>
        <property type="gene ID" value="BN566_00248"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000310">
        <property type="entry name" value="Orn/Lys/Arg_deCO2ase_major_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008286">
        <property type="entry name" value="Prn/Lys/Arg_de-COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036633">
        <property type="entry name" value="Prn/Lys/Arg_de-COase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01276">
        <property type="entry name" value="OKR_DC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03711">
        <property type="entry name" value="OKR_DC_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55904">
        <property type="entry name" value="SSF55904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OKR_DC_1" evidence="4">
        <location>
            <begin position="3"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="OKR_DC_1_C" evidence="5">
        <location>
            <begin position="353"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="449"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ84335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="468" mass="50581" checksum="4D2A26946C90CD4F" modified="2013-07-24" version="1">MKTPIYDALQAYADRQPIRFHMPGHKGHSAIPAWNPLYALDITEISGADSLLEADGIIAESEQIATALFFSAGTVYSCAGSTGCIQTMLTLMKQENRTIIAARNVHRSFLNACILLGLTVKWVYPETNNGLLSGQYTPEQFAAVLAETKEPACVYVTSPDYLGKTADIAGIAAVCKQYNNARFLVDNAHGAYLAFLKDGKHPIQNGADFCCDSAHKTLPCLTGCAYLHAAHEEDVPRIKDAMSMFVSTSPSYLMLLSLDVCNAYLAGKIRSDLERICKRAAALKKRLSEKYVFLDGDPLHLTIDAAASKLNGLDLAARLERADCVPEYADRETVVLLLSPYNVPEELDVLEVTLHMIVAESYPKTQTAEILLPQPEVVCNMREAALGAQEVIPVAESIGRICAAVKVPCPPAVPIVCSGERIDAQSAAVLQHYGISEIAVVKEMDSGESVAAVAEEPDLETESTTSEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>R5V227</accession>
    <name>R5V227_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Fructose-1 6-bisphosphate aldolase class II</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00277</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="3">Binds 2 Zn(2+) ions per subunit. One is catalytic and the other provides a structural contribution.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000221">
        <property type="protein sequence ID" value="CCZ84370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00277"/>
    <dbReference type="EnsemblBacteria" id="CCZ84370">
        <property type="protein sequence ID" value="CCZ84370"/>
        <property type="gene ID" value="BN566_00277"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004332">
        <property type="term" value="F:fructose-bisphosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030388">
        <property type="term" value="P:fructose 1,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00947">
        <property type="entry name" value="TBP_aldolase_IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000771">
        <property type="entry name" value="FBA_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011289">
        <property type="entry name" value="Fruc_bis_ald_class-2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01116">
        <property type="entry name" value="F_bP_aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001359">
        <property type="entry name" value="F_bP_aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00167">
        <property type="entry name" value="cbbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01859">
        <property type="entry name" value="fruc_bis_ald"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00806">
        <property type="entry name" value="ALDOLASE_CLASS_II_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Dihydroxyacetone phosphate binding" evidence="2">
        <location>
            <begin position="208"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Dihydroxyacetone phosphate binding" evidence="2">
        <location>
            <begin position="229"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR001359-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR001359-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001359-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="287" mass="30502" checksum="C886DDDDD945838E" modified="2013-07-24" version="1">MLVSAKDMMEKARDGKYAVGQFNINNLEWTKAILNTAEKLQSPVILGVSGGAGKYMTGFKTVAAMVKAMDETLGITVPVALHLDHGTYDQCYDCIKAGFTSIMFDGSHFPIEENLAKTKELVAVAHKLGLSIEAEVGAIGGEEDGVIGRGECADPQECKTIADLGVDILAAGIGNIHGVYPANWEGLSFETLAAVKEAVGDMPLVLHGGTGIPDDQITKAISLGVAKINVNTECQLVFAEATRKYIEEGKDLKGKGFDPRKLLLPGYEAILAKVEEKMNLFGSVGKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>R5V260</accession>
    <name>R5V260_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">UDP-N-acetylglucosamine 1-carboxyvinyltransferase</fullName>
            <ecNumber evidence="7">2.5.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Enoylpyruvate transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">UDP-N-acetylglucosamine enolpyruvyl transferase</fullName>
            <shortName evidence="7">EPT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">murA</name>
        <name evidence="9" type="ORF">BN566_00312</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine</text>
            <dbReference type="Rhea" id="RHEA:18681"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:68483"/>
            <dbReference type="EC" id="2.5.1.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the EPSP synthase family. MurA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.7" evidence="7"/>
    <dbReference type="EMBL" id="CBAR010000224">
        <property type="protein sequence ID" value="CCZ84405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00312"/>
    <dbReference type="EnsemblBacteria" id="CCZ84405">
        <property type="protein sequence ID" value="CCZ84405"/>
        <property type="gene ID" value="BN566_00312"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008760">
        <property type="term" value="F:UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019277">
        <property type="term" value="P:UDP-N-acetylgalactosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01555">
        <property type="entry name" value="UdpNAET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00111">
        <property type="entry name" value="MurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001986">
        <property type="entry name" value="Enolpyruvate_Tfrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036968">
        <property type="entry name" value="Enolpyruvate_Tfrase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005750">
        <property type="entry name" value="UDP_GlcNAc_COvinyl_MurA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00275">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="SSF55205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01072">
        <property type="entry name" value="murA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1 7" id="KW-0132">Cell division</keyword>
    <keyword evidence="3 7" id="KW-0133">Cell shape</keyword>
    <keyword evidence="6 7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="7" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="EPSP_synthase" evidence="8">
        <location>
            <begin position="7"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphoenolpyruvate binding" evidence="7">
        <location>
            <begin position="22"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="UDP-N-acetylglucosamine binding" evidence="7">
        <location>
            <begin position="122"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="305"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="2-(S-cysteinyl)pyruvic acid O-phosphothioketal" evidence="7">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ84405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="427" mass="46038" checksum="03C9C25E68501772" modified="2013-07-24" version="1">MDKFVIHGGHRLSGEIEVSGAKNAAVALIPAVLLCDEPCILENVPEISDVSISLRILYEMGAQVDYINRTTVRISTVGLKNFCVPYESARRMRASYYFLGALLGKFGKARVSMPGGCPLGDRPIDQHLKAFSALGAKCDIQHGMVDLTADKLTGNQIYLDVVSVGATMNAMLAAVRAEGLTVIENAAKEPHIVDLANFLNSMGADIMGAGTDVIKVRGVQYLHGTTYSVIPDQIEAGTFMIAAAATHGDVLVKNVIPKHLEPITMKLRKIGVNIEEFDDSVRVWVDGPLVRTNVKTMPHPGFPTDMQPQITTLLTLAEGTSIITEGVWEQRFRYVDELRRMGADISVDGKVAVVEGTGKLTGAPVKACDLRAGAALIIAGLAAKGITEIEDIYHIERGYSCLDEKLRQVGADIQKVSTPVSTLREAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>R5V2A6</accession>
    <name>R5V2A6_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 8">Stage 0 sporulation protein A homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BN566_00531</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 8">May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8 9">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="8 9">Binds 1 Ca(2+) ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000267">
        <property type="protein sequence ID" value="CCZ84678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00531"/>
    <dbReference type="EnsemblBacteria" id="CCZ84678">
        <property type="protein sequence ID" value="CCZ84678"/>
        <property type="gene ID" value="BN566_00531"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051606">
        <property type="term" value="P:detection of stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042173">
        <property type="term" value="P:regulation of sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030435">
        <property type="term" value="P:sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014879">
        <property type="entry name" value="Spo0A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012052">
        <property type="entry name" value="Spore_0_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08769">
        <property type="entry name" value="Spo0A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002937">
        <property type="entry name" value="Res_reg_Spo0A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02875">
        <property type="entry name" value="spore_0_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 8" id="KW-0010">Activator</keyword>
    <keyword evidence="3 8 9" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0678">Repressor</keyword>
    <keyword evidence="4 8" id="KW-0749">Sporulation</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Response regulatory" evidence="11">
        <location>
            <begin position="6"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="10">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR002937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR002937-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CCZ84678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="256" mass="28981" checksum="73B650A9056FF84C" modified="2013-07-24" version="1">MDSKIKVLIGDDSAEYGIACASSLRAAGLYVITRRKDGRTLVDAIQHDAPDVVIVDAVLPNLDAMELMRRVRSGTGKQPYFIVTSAYDSAFIEKQAMESGASYFMLKPFNLEMLGQRIIRLVQGDQKEPTKEIEEDLEFVVTETIHQLGVPAHIKGYHYLRVAILLSIDNQELLESVTKMLYPAVAKHFDTTSSRVERAIRHAIEIAWDRGDLDTLNTFFGYTVNTAKGKPTNSEFIALITDKLRLQYRRNKRRWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>R5V2V5</accession>
    <name>R5V2V5_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pyrophosphate--fructose 6-phosphate 1-phosphotransferase</fullName>
            <ecNumber evidence="8">2.7.1.90</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">6-phosphofructokinase, pyrophosphate dependent</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">PPi-dependent phosphofructokinase</fullName>
            <shortName evidence="8">PPi-PFK</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Pyrophosphate-dependent 6-phosphofructose-1-kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">pfp</name>
        <name evidence="10" type="ORF">BN566_00458</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the phosphorylation of D-fructose 6-phosphate, the first committing step of glycolysis. Uses inorganic phosphate (PPi) as phosphoryl donor instead of ATP like common ATP-dependent phosphofructokinases (ATP-PFKs), which renders the reaction reversible, and can thus function both in glycolysis and gluconeogenesis. Consistently, PPi-PFK can replace the enzymes of both the forward (ATP-PFK) and reverse (fructose-bisphosphatase (FBPase)) reactions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>beta-D-fructose 6-phosphate + diphosphate = beta-D-fructose 1,6-bisphosphate + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13613"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="EC" id="2.7.1.90"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="8">Non-allosteric.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Clade 'B2' sub-subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.90" evidence="8"/>
    <dbReference type="EMBL" id="CBAR010000256">
        <property type="protein sequence ID" value="CCZ84605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00458"/>
    <dbReference type="EnsemblBacteria" id="CCZ84605">
        <property type="protein sequence ID" value="CCZ84605"/>
        <property type="gene ID" value="BN566_00458"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00182"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003872">
        <property type="term" value="F:6-phosphofructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047334">
        <property type="term" value="F:diphosphate-fructose-6-phosphate 1-phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01978">
        <property type="entry name" value="Phosphofructokinase_II_B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022953">
        <property type="entry name" value="ATP_PFK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000023">
        <property type="entry name" value="Phosphofructokinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035966">
        <property type="entry name" value="PKF_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011404">
        <property type="entry name" value="PPi-PFK_XF0274"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00365">
        <property type="entry name" value="PFK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036483">
        <property type="entry name" value="PFK_XF0274"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00476">
        <property type="entry name" value="PHFRCTKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53784">
        <property type="entry name" value="SSF53784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="5 8 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PFK" evidence="9">
        <location>
            <begin position="2"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="140"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="186"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalytic activity and substrate specificity; stabilizes the transition state when the phosphoryl donor is PPi; prevents ATP from binding by mimicking the alpha-phosphate group of ATP" evidence="8">
        <location>
            <position position="113"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalytic activity; stabilizes the transition state when the phosphoryl donor is PPi" evidence="8">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ84605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="406" mass="44499" checksum="634F6EABC80E860E" modified="2013-07-24" version="1">MNIAVAQSGGPTCAINASLVGVFSEALKEPSIDAIFGSINGIEGMIDDHLIDLKTMIRTNEDMEMLLQTPSTVLGSCRYKLKDWHDDDTVYQKIIHCFEQRQIGAFFYIGGNDSMDTVLKLSEYVKEKGLNTKIIGIPKTIDNDLCITDHTPGFGSAAKYVATTMQEITRDSSVYSAPSVTIVEIMGRNAGWLTASSVVLHANGEVAPHLIYVPERKFSIEQFLEDTRQQMAKHKAVIIAISEGVADPEAAAFQSGVTDEFGHKYLSGIGKYLEQVVREQIGCKVRSIELNVMQRCSSHLCSKTDIDESVQIGAAGVRAALAGETGIVMTFRRVSDQPYAIVVKSTSAANIANQERFLPPAYINDAGNNITEAAWSYFLPLIQGECNIRLHNGLPVHFKMQESVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5V338</accession>
    <name>R5V338_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Stage V sporulation protein AE</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00548</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000268">
        <property type="protein sequence ID" value="CCZ84695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00548"/>
    <dbReference type="EnsemblBacteria" id="CCZ84695">
        <property type="protein sequence ID" value="CCZ84695"/>
        <property type="gene ID" value="BN566_00548"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014204">
        <property type="entry name" value="Spore_V_AE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005562">
        <property type="entry name" value="SpoVA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38450">
        <property type="entry name" value="PTHR38450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03862">
        <property type="entry name" value="SpoVAC_SpoVAEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02839">
        <property type="entry name" value="spore_V_AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="119" mass="12165" checksum="46E9280FAF754F41" modified="2013-07-24" version="1">MIQDCWIAFLVGGLLCAIGQVLIDYTKLTPARILTGYVVSGVLLSAVGLYPKLIDWAGGGASVPLTGFGNLLAQGVRDAIREDGFLGVFTGGLQAAAGGLSAALGFGLLAAICFHPKDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5V3Z8</accession>
    <name>R5V3Z8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulators containing a DNA-binding HTH domain and an aminotransferase domain (MocR family) and their eukaryotic orthologs</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00644</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000012">
        <property type="protein sequence ID" value="CCZ82839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00644"/>
    <dbReference type="EnsemblBacteria" id="CCZ82839">
        <property type="protein sequence ID" value="CCZ82839"/>
        <property type="gene ID" value="BN566_00644"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="2">
        <location>
            <begin position="63"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ82839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="398" mass="44406" checksum="7FE0B92F722F40DB" modified="2013-07-24" version="1">MQFQFSNDVAKLQPSAIREILKFTSMPGVISFAAGNPAPEAFPTELIADLSSKLLREHPVLAMQYSITEGYPELREQLKQWMTEKHCFSPETDDLIITSGAQQANELSCKVLLNPGDTLICEAPSFIGSLNAFRSYGVNLVSVPLEEDGMDLAALEHTLQTEPNCKLIYIIPNFQNPTGRVTSLEKRKGIYELACKYNVAILEDDPYGELRISGESVPSLKSMDTEGRVLYAGSFSKILAPGFRVGYLSAPKELVQKVVVCKQVSDVHTNIWAQIMCSEFLKNVDLQEHLEGLRKIYRHKCTLMADTMEACFSKKVAWKKPEGGLFIWCTLPDDCDMPEFCKKAVSEYKIAVVPGNAFLVDESQPTTSFRLNFSTPTDEQIVTGITILGRMTKELFSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5V490</accession>
    <name>R5V490_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_00734</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000029">
        <property type="protein sequence ID" value="CCZ82934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00734"/>
    <dbReference type="EnsemblBacteria" id="CCZ82934">
        <property type="protein sequence ID" value="CCZ82934"/>
        <property type="gene ID" value="BN566_00734"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16913">
        <property type="entry name" value="YkuD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038054">
        <property type="entry name" value="LD_TPept-like_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005490">
        <property type="entry name" value="LD_TPept_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022029">
        <property type="entry name" value="PG-bd_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038063">
        <property type="entry name" value="Transpep_catalytic_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12229">
        <property type="entry name" value="PG_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03734">
        <property type="entry name" value="YkuD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141523">
        <property type="entry name" value="SSF141523"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143985">
        <property type="entry name" value="SSF143985"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="153"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="PG_binding_4" evidence="6">
        <location>
            <begin position="345"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="YkuD" evidence="5">
        <location>
            <begin position="484"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="35"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="87"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ82934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="608" mass="67004" checksum="C1ECEE72522B95C8" modified="2013-07-24" version="1">MNETQKDGRSTYGSPAPLSREQMQQQMQAAFQDGSNRPRKKKQPEQRRISDMPNGKPAQRGKRGNQPEPMPRSVGQAKAEKLRQIKASVASASQGIYNEETPSATAAEAHRYVHRRSSAPPPPVPPRNRNSRPQEPEYYGGSDVASSGKGGKIAGIIVLVLVVLLLIGYLIGYFSYRSKLLPKTYVNGVNVSGMTLADAEQKILNSASTEGITFIKKSGEKVHFDGAEFGSTVSFQDGAMDEVTSQSHVLWFTHLLHKSEYTVSVVNSYSESNLSNLILSYSWGTTPPTDAHIQKASDGTYSIVPEDNGDMIDATILMNYTLEQMREGNNTIKLEDADCYLQAEVKSEDLQAALDAANSLAGLTITYDFDDRQEVLDTSTIADWITTDTDGKISVDENKVKAWVNENLVKYDTFVPGYTRTFHSTMQGTIEVPLGDYGIYGWKTDVDATVEKLKEYILAGESVTVEPEYSQRGYCRATDDIGDSYAEVDITNQKVFVYKDGELVIESDCVTGLASDPERATPPGVYRVWTMEKDVVLGTYAVQGYEAPVNYWMNFTEIGIGFHDLARVAYGGDIYKTNGSHGCINLPLDVAAEMFDTIEVGYPVIVIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5V4V9</accession>
    <name>R5V4V9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00931</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000063">
        <property type="protein sequence ID" value="CCZ83144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83144">
        <property type="protein sequence ID" value="CCZ83144"/>
        <property type="gene ID" value="BN566_00931"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025346">
        <property type="entry name" value="DUF4250"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14056">
        <property type="entry name" value="DUF4250"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="61" mass="7076" checksum="CB49308C5B6DF1E2" modified="2013-07-24" version="1">MALPKDPYILLSYLNTQLRDKYASLAALCDDLEEDQAALEAIMQTVQYHYDVNRNQFIHNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>R5V5G4</accession>
    <name>R5V5G4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Site-specific recombinase XerD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN566_01150</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Site-specific tyrosine recombinase, which acts by catalyzing the cutting and rejoining of the recombining DNA molecules.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 'phage' integrase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000093">
        <property type="protein sequence ID" value="CCZ83374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01150"/>
    <dbReference type="EnsemblBacteria" id="CCZ83374">
        <property type="protein sequence ID" value="CCZ83374"/>
        <property type="gene ID" value="BN566_01150"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044068">
        <property type="entry name" value="CB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004107">
        <property type="entry name" value="Integrase_SAM-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14659">
        <property type="entry name" value="Phage_int_SAM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51900">
        <property type="entry name" value="CB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core-binding (CB)" evidence="8">
        <location>
            <begin position="97"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="Tyr recombinase" evidence="7">
        <location>
            <begin position="203"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="416"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="420"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ83374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="439" mass="50950" checksum="589A19283DA847D7" modified="2013-07-24" version="1">MKASLPFKYIIAEKKGKNYVVFDFKDDSGKRKRKWVTTDLPVDCSYKALTAKVNVIVAKFYEEFLTGSLTKVKETPKEKTELSEEVLSDDAVCKTSFEFTAFLDYWLETIRPTIARTSYESYNRYITRIKNYFDERYPHLLLGNLTALQLQQFYNDKFNSGLSGNSVKHYHANIHKALKYAVKMDMLDVNVADKVELPKIQKFEANFYNKDELEQLFEVFKGDRLELVVHIAAYYGLRKSEIIGLKWDSVNFEEKKLTVRRKVSSTYGGGKEMIFVENQLKTESSVRTFPLIPHIEQMLIERKNLEEYYSKLLGKDFDREYDGFVCRDNFGKLITPNFVTTHFKYIIKKNKLKHIRFHDLRHSCASLLLANGVSMKAIQEWLGHSTFNVTANFYSHLDFHSKVESAETIAKVLGGDSTDTEQAIHDKGSEDEKNRKSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>R5V6S1</accession>
    <name>R5V6S1_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">3-phosphoshikimate 1-carboxyvinyltransferase</fullName>
            <ecNumber evidence="7">2.5.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">5-enolpyruvylshikimate-3-phosphate synthase</fullName>
            <shortName evidence="7">EPSP synthase</shortName>
            <shortName evidence="7">EPSPS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">aroA</name>
        <name evidence="9" type="ORF">BN566_01549</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>3-phosphoshikimate + phosphoenolpyruvate = 5-O-(1-carboxyvinyl)-3-phosphoshikimate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21256"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57701"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
            <dbReference type="EC" id="2.5.1.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the EPSP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.19" evidence="7"/>
    <dbReference type="EMBL" id="CBAR010000165">
        <property type="protein sequence ID" value="CCZ83814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01549"/>
    <dbReference type="EnsemblBacteria" id="CCZ83814">
        <property type="protein sequence ID" value="CCZ83814"/>
        <property type="gene ID" value="BN566_01549"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00089"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003866">
        <property type="term" value="F:3-phosphoshikimate 1-carboxyvinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01556">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00210">
        <property type="entry name" value="EPSP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001986">
        <property type="entry name" value="Enolpyruvate_Tfrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036968">
        <property type="entry name" value="Enolpyruvate_Tfrase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006264">
        <property type="entry name" value="EPSP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023193">
        <property type="entry name" value="EPSP_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00275">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000505">
        <property type="entry name" value="EPSPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="SSF55205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01356">
        <property type="entry name" value="aroA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00885">
        <property type="entry name" value="EPSP_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="EPSP_synthase" evidence="8">
        <location>
            <begin position="7"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Shikimate-3-phosphate binding" evidence="7">
        <location>
            <begin position="20"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphoenolpyruvate" evidence="7">
        <location>
            <begin position="89"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Shikimate-3-phosphate binding" evidence="7">
        <location>
            <begin position="161"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="293"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="321"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="320"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="324"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="366"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="392"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ83814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="414" mass="44705" checksum="709780046A245B9A" modified="2013-07-24" version="1">MNIQVEPRTLSGTVSIPASKSMTHRMLICAALAEGTSVIEHVSFSKDITATMEALSALGADFETNGSTITVTGIHQVATTQTAEMNCCESGSTLRFLIPVAAALGVHATFHGQGRLPQRPITAYVRELSKNGITFDYQNTMPFTIHGQLQSGTYYLEGDVSSQYITGLLFALPLLKGDSEIVMLSPLQSKPYVDMTIACMQQFGFVVRETEQGYFVPGGQRAHACSGTVEGDYSQAAFFAVANAIGNQITMTNLNPDSVQGDKIIIEIAEKMCYNRNNHKPACFTLDAADIPDLVPILAVLATFGTHPSEIHGAARLKIKESDRLQATADLLNRLGGQVTILEDGLRIQPVERLHGGKVDSYGDHRIAMCAAIAATRCTAPVVIQGGECIEKSYPQFYEDYNRLGGNAHVIVLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5V6S7</accession>
    <name>R5V6S7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Predicted membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_01554</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GtrA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000165">
        <property type="protein sequence ID" value="CCZ83819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01554"/>
    <dbReference type="EnsemblBacteria" id="CCZ83819">
        <property type="protein sequence ID" value="CCZ83819"/>
        <property type="gene ID" value="BN566_01554"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007267">
        <property type="entry name" value="GtrA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04138">
        <property type="entry name" value="GtrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="26"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="96"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="126"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="GtrA" evidence="7">
        <location>
            <begin position="29"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="162" mass="18654" checksum="DAAB37BEAFA1D024" modified="2013-07-24" version="1">MNQLILWVVKHLPKPLQNIYFKYEKGLLYCFYGALTTLVSMITKLVPMYFLGEGKLSVTFCTVFSWICAVTFAFFTNKKYVFQSETHTKKAFWTEFISFYSARGASLFLDWGISVLGISILGWNKYLVTIAAQVIILLVNYAFSKLFVFKNRENPTDAAESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>R5V780</accession>
    <name>R5V780_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Oxidoreductase FAD/NAD(P)-binding domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01683</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="1">Binds 1 [2Fe-2S] cluster per subunit.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000175">
        <property type="protein sequence ID" value="CCZ83954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01683"/>
    <dbReference type="EnsemblBacteria" id="CCZ83954">
        <property type="protein sequence ID" value="CCZ83954"/>
        <property type="gene ID" value="BN566_01683"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006221">
        <property type="term" value="P:pyrimidine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008333">
        <property type="entry name" value="Cbr1-like_FAD-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012165">
        <property type="entry name" value="Cyt_c3_hydrogenase_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019480">
        <property type="entry name" value="Dihydroorotate_DH_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10418">
        <property type="entry name" value="DHODB_Fe-S_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00970">
        <property type="entry name" value="FAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006816">
        <property type="entry name" value="Cyc3_hyd_g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="SSF52343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="SSF63380"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="2">
        <location>
            <begin position="6"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR006816-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="275" mass="30685" checksum="BF9F8C09D6CDB072" modified="2013-07-24" version="1">MRPETLIPKIGVVTDIRVDTPDVKTFRVVGLDGKKVFEHIPGQCAMLSVPGVGEGMFSITSSPTNEEFMEFSIKKCGCLTSWLHMMDVGQQITIRGPYGNGFPVESALKGKDLLFIAGGIGLAPLRSVINYVRDKKDNYGSVHIIYGSRSADDLVDYQEILNEWQTDENVRVDLTIDRPQEGWDGHVGFIPSFVEELKPDLSRTVLICGPPIMIKFTLDGLKKMGFQESQVYTTMELRMKCAIGKCGRCNIGNKYVCKDGPVFRFDELSTLPNEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7X3</accession>
    <name>R5V7X3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA pseudouridine synthase A</fullName>
            <ecNumber evidence="4">5.4.99.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">tRNA pseudouridine(38-40) synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">tRNA pseudouridylate synthase I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">tRNA-uridine isomerase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">truA</name>
        <name evidence="9" type="ORF">BN566_01949</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 7">
            <text>uridine(38/39/40) in tRNA = pseudouridine(38/39/40) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:22376"/>
            <dbReference type="Rhea" id="RHEA-COMP:10085"/>
            <dbReference type="Rhea" id="RHEA-COMP:10087"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 7">Belongs to the tRNA pseudouridine synthase TruA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.12" evidence="4"/>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01949"/>
    <dbReference type="EnsemblBacteria" id="CCZ84224">
        <property type="protein sequence ID" value="CCZ84224"/>
        <property type="gene ID" value="BN566_01949"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106029">
        <property type="term" value="F:tRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02570">
        <property type="entry name" value="PseudoU_synth_EcTruA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.660">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00171">
        <property type="entry name" value="TruA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001406">
        <property type="entry name" value="PsdUridine_synth_TruA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020097">
        <property type="entry name" value="PsdUridine_synth_TruA_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020095">
        <property type="entry name" value="PsdUridine_synth_TruA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020094">
        <property type="entry name" value="TruA/RsuA/RluB/E/F_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142">
        <property type="entry name" value="PTHR11142"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01416">
        <property type="entry name" value="PseudoU_synth_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001430">
        <property type="entry name" value="tRNA_psdUrid_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00071">
        <property type="entry name" value="hisT_truA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4 7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="PseudoU_synth_1" evidence="8">
        <location>
            <begin position="9"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="PseudoU_synth_1" evidence="8">
        <location>
            <begin position="143"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4 5">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4 6">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001430-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001430-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ84224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="270" mass="30296" checksum="445F6EFE375A5B3F" modified="2013-07-24" version="1">MRTLKVMMAYCGTRYHGYQRQNNANTVQAVVEAAVSKLLNTPTVIAGCSRTDTGVHAEQFCFSLTTENPIPCRNFVRGLNGYLPEDISILSCEEVKDTFHARFCCQAKTYRYWIHNSESKNPFAADRALHYRRALRIDQMQAAANLLKGTHDFASFCSGCTENKDTVRTIYQFDIKMQGTDCFILVKGNGFLYNMIRILVGTLLSVSEGELSVSEIPEILAARNRIYAGRTVQPYGLYLHEIFYDDAAYRLPQGDAFFGSAVSGGERNGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>R5V813</accession>
    <name>R5V813_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glucanase</fullName>
            <ecNumber evidence="4">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_00198</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 8 (cellulase D) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="4"/>
    <dbReference type="EMBL" id="CBAR010000204">
        <property type="protein sequence ID" value="CCZ84269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00198"/>
    <dbReference type="EnsemblBacteria" id="CCZ84269">
        <property type="protein sequence ID" value="CCZ84269"/>
        <property type="gene ID" value="BN566_00198"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002105">
        <property type="entry name" value="Dockerin_1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002037">
        <property type="entry name" value="Glyco_hydro_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019834">
        <property type="entry name" value="Glyco_hydro_8_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00404">
        <property type="entry name" value="Dockerin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01270">
        <property type="entry name" value="Glyco_hydro_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00735">
        <property type="entry name" value="GLHYDRLASE8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="SSF48208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00812">
        <property type="entry name" value="GLYCOSYL_HYDROL_F8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004394974" description="Glucanase" evidence="5">
        <location>
            <begin position="30"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="6">
        <location>
            <begin position="421"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ84269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="481" mass="53525" checksum="CC46CB1E616F2D63" modified="2013-07-24" version="1">MNTAKKWIAAFSAGLFSLSACPLLTSVQAANADRKQVLQDTYQQWKKSYVTEDTYISSGKPQYYVSYEENRYAGDGVSVPVTVSEAHGYGMLITVCMADYDAQAKDTFDGMYRYYRAHLSDIGENLMSWQQCDNGSSLIDGATDGAMQGGDADSATDGDLDIAYSLLLADQRWGSSEETPYRETALAMIQDIMDYEVNQEDWLLQLGDWVHECDQSDSYYFATRSSDFILQYFPVFEAITGDKRWGKLYDGTCAVIESITAEQSTGLLPDFIVKNAAGKFVPAPENFLEDVTDGTYAYNSCRTPWRLGMDLLYPSEKGANDTVKTVIHKLNSWIQTETDGDPENIVAGYELDGTPTQDYDDLCFTAPFLVAAACEDSASSWEQALWDTLADYGTDVYFGDTIRILCMISVTGNWLIPETTTDSTKGDLDGDGTATVSDLVLLNRYLLRLESLTTEQGNRADWNDDQQITVVDAMLMRRSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5V845</accession>
    <name>R5V845_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00217</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000210">
        <property type="protein sequence ID" value="CCZ84304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84304">
        <property type="protein sequence ID" value="CCZ84304"/>
        <property type="gene ID" value="BN566_00217"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013321">
        <property type="entry name" value="Arc_rbn_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="SSF47598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="64" mass="7613" checksum="70D9D7CD8CB04E56" modified="2013-07-24" version="1">MKAKRQFTLRLDEALLNKLGYIAEYEGRTKNKEIQHLIQQHIVHFEQEHGAIDLREIQQEEPVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>R5V849</accession>
    <name>R5V849_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6 7">Protein RecA</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6 7">Recombinase A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">recA</name>
        <name evidence="12" type="ORF">BN566_00222</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with LexA causing its activation and leading to its autocatalytic cleavage.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 8">Belongs to the RecA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000211">
        <property type="protein sequence ID" value="CCZ84309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00222"/>
    <dbReference type="EnsemblBacteria" id="CCZ84309">
        <property type="protein sequence ID" value="CCZ84309"/>
        <property type="gene ID" value="BN566_00222"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00983">
        <property type="entry name" value="recA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00268">
        <property type="entry name" value="RecA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013765">
        <property type="entry name" value="DNA_recomb/repair_RecA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020584">
        <property type="entry name" value="DNA_recomb/repair_RecA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023400">
        <property type="entry name" value="RecA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020587">
        <property type="entry name" value="RecA_monomer-monomer_interface"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45900">
        <property type="entry name" value="PTHR45900"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00154">
        <property type="entry name" value="RecA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00142">
        <property type="entry name" value="RECA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54752">
        <property type="entry name" value="SSF54752"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02012">
        <property type="entry name" value="tigrfam_recA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00321">
        <property type="entry name" value="RECA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50163">
        <property type="entry name" value="RECA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6 8" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="6 7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 6 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 6 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0742">SOS response</keyword>
    <feature type="domain" description="RECA_2" evidence="10">
        <location>
            <begin position="51"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="RECA_3" evidence="11">
        <location>
            <begin position="215"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="349"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="349"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="364"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="395"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="81"/>
            <end position="88"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CCZ84309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="412" mass="44512" checksum="919216906AC295D9" modified="2013-07-24" version="1">MAKELKKNKAQMVMPESKEDKKKALESAIAHILKTYGSGAVMRLGQANTMQVDAIPTGSMTLDLALGIGGVPRGRIVELYGPESSGKTTVALHIAAEAQKQGGEVAFIDVEHALDPVYARALGVDIDNLLVSQPDSGEQALEIAEALVRSGAIDVLIVDSVAAMTTKAEIEGEMGDSHVGMLARLMSQAMRKLTSIISKSNCVAIFINQIREKIGVVYGNPETTPGGRALKFYASVRMEVRKGEAIKDGTTIIGNRTRVKIVKNKVAPPFRECEFDILYGKGISRSGEVLDLAVDLDIINKSGSWFSYEGQRLGQGRDNIKELLQNTPQLMQEVEEKIKKKLAEQKAAAVKAAEQKRNGRKPAESNNALEQAAEQAAAAQMQQANLNALDMESDADLDIAAETDFSEFEPTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>R5V875</accession>
    <name>R5V875_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pyridoxal 5'-phosphate synthase subunit PdxS</fullName>
            <shortName evidence="3">PLP synthase subunit PdxS</shortName>
            <ecNumber evidence="3">4.3.3.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Pdx1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">pdxS</name>
        <name evidence="6" type="ORF">BN566_00242</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the formation of pyridoxal 5'-phosphate from ribose 5-phosphate (RBP), glyceraldehyde 3-phosphate (G3P) and ammonia. The ammonia is provided by the PdxT subunit. Can also use ribulose 5-phosphate and dihydroxyacetone phosphate as substrates, resulting from enzyme-catalyzed isomerization of RBP and G3P, respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>aldehydo-D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = H(+) + 3 H2O + L-glutamate + phosphate + pyridoxal 5'-phosphate</text>
            <dbReference type="Rhea" id="RHEA:31507"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="4.3.3.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; pyridoxal 5'-phosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">In the presence of PdxT, forms a dodecamer of heterodimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3 4">Belongs to the PdxS/SNZ family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.3.6" evidence="3"/>
    <dbReference type="EMBL" id="CBAR010000214">
        <property type="protein sequence ID" value="CCZ84329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00242"/>
    <dbReference type="EnsemblBacteria" id="CCZ84329">
        <property type="protein sequence ID" value="CCZ84329"/>
        <property type="gene ID" value="BN566_00242"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00245"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036381">
        <property type="term" value="F:pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042823">
        <property type="term" value="P:pyridoxal phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04727">
        <property type="entry name" value="pdxS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01824">
        <property type="entry name" value="PdxS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001852">
        <property type="entry name" value="PdxS/SNZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033755">
        <property type="entry name" value="PdxS/SNZ_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31829">
        <property type="entry name" value="PTHR31829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01680">
        <property type="entry name" value="SOR_SNZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029271">
        <property type="entry name" value="Pdx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00343">
        <property type="entry name" value="TIGR00343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01235">
        <property type="entry name" value="PDXS_SNZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51129">
        <property type="entry name" value="PDXS_SNZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0704">Schiff base</keyword>
    <feature type="domain" description="SOR_SNZ" evidence="5">
        <location>
            <begin position="7"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="D-ribose 5-phosphate binding" evidence="3">
        <location>
            <begin position="235"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with D-ribose 5-phosphate" evidence="3">
        <location>
            <position position="81"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ84329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="292" mass="31367" checksum="3C01F77D15FE3CFC" modified="2013-07-24" version="1">MENQTQVKLNRELAQMLKGGVIMDVTTPEQAKIAEAAGACAVMALERIPADIRAAGGVSRMSDPKMIKGIQNAVSIPVMAKCRIGHFAEAQILQAIEIDYIDESEVLSPADDVHHIDKTAFKVPFVCGAKDLGEALRRIAEGATMIRTKGEPGTGDIVQAVRHMRMMNKEIARLTSMRTDELYEAAKQLQVPYDLVCYVHENGRLPVVNFAAGGVATPADAALMMQLGAEGVFVGSGIFKSGNPEKRASAIVKAVTNYNDAAMLAELSEDLGEAMVGINEQEIALLMAERGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>R5V8L5</accession>
    <name>R5V8L5_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosomal RNA large subunit methyltransferase H</fullName>
            <ecNumber evidence="1">2.1.1.177</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">23S rRNA (pseudouridine1915-N3)-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">23S rRNA m3Psi1915 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">rRNA (pseudouridine-N3-)-methyltransferase RlmH</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rlmH</name>
        <name evidence="2" type="ORF">BN566_00353</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>pseudouridine(1915) in 23S rRNA + S-adenosyl-L-methionine = H(+) + N(3)-methylpseudouridine(1915) in 23S rRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10221"/>
            <dbReference type="Rhea" id="RHEA-COMP:10222"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:74486"/>
            <dbReference type="EC" id="2.1.1.177"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNA methyltransferase RlmH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.177" evidence="1"/>
    <dbReference type="EMBL" id="CBAR010000233">
        <property type="protein sequence ID" value="CCZ84454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00353"/>
    <dbReference type="EnsemblBacteria" id="CCZ84454">
        <property type="protein sequence ID" value="CCZ84454"/>
        <property type="gene ID" value="BN566_00353"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070038">
        <property type="term" value="F:rRNA (pseudouridine-N3-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18081">
        <property type="entry name" value="RlmH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00658">
        <property type="entry name" value="23SrRNA_methyltr_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003742">
        <property type="entry name" value="RlmH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33603">
        <property type="entry name" value="PTHR33603"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02590">
        <property type="entry name" value="SPOUT_MTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004505">
        <property type="entry name" value="MT_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="1 2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="1">
        <location>
            <begin position="126"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="158" mass="17750" checksum="C530D7F2805B7C5F" modified="2013-07-24" version="1">MQLIQLLTYGKLKESYLRAACSEYEKRLSRFCKLQCVELEPVALPERPSEKEIAAALEKEAQPLLKRKKGFTIAMCIEGKQLDSPQFAQIFQRAAVQGDAAVTFFIGSSYGLADSVKQAANLRLSMSAMTFPHQLARVMLLEQIYRGYQILGGSPYHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5V8Q3</accession>
    <name>R5V8Q3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Malonyl CoA-acyl carrier protein transacylase</fullName>
            <ecNumber evidence="3">2.3.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_00383</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 3">
            <text>holo-[ACP] + malonyl-CoA = CoA + malonyl-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:41792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="EC" id="2.3.1.39"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the fabD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.39" evidence="3"/>
    <dbReference type="EMBL" id="CBAR010000240">
        <property type="protein sequence ID" value="CCZ84484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00383"/>
    <dbReference type="EnsemblBacteria" id="CCZ84484">
        <property type="protein sequence ID" value="CCZ84484"/>
        <property type="gene ID" value="BN566_00383"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004314">
        <property type="term" value="F:[acyl-carrier-protein] S-malonyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014043">
        <property type="entry name" value="Acyl_transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024925">
        <property type="entry name" value="Malonyl_CoA-ACP_transAc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004410">
        <property type="entry name" value="Malonyl_CoA-ACP_transAc_FabD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016036">
        <property type="entry name" value="Malonyl_transacylase_ACP-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00698">
        <property type="entry name" value="Acyl_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000446">
        <property type="entry name" value="Mct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00827">
        <property type="entry name" value="PKS_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="SSF52151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55048">
        <property type="entry name" value="SSF55048"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00128">
        <property type="entry name" value="fabD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PKS_AT" evidence="5">
        <location>
            <begin position="5"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR000446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000446-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ84484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="302" mass="31970" checksum="2F0C31DB39EFD2AD" modified="2013-07-24" version="1">MTVSLFSGQGSQYPGMGLDLLAAHPALAVIYDTASSILGFDLKEIIQNSESETLSRTIYAQPAIMATSLCCLGAAREQGYTFNAVAGHSLGEYAAMVAAGMLSLEDGFRVIKARSEAMDAAAQQADGAMCAIMKLKPEEVEEICAATEGYVVPANYNSSVQTVIAGDKAAVEAAAAVCKEKKARAIPLAVASAFHTKLMQPAADAFYEAIQGVSFQKPQVRFYSNVLGTELTDFSDMPTLLAKHIVSPVRFTQELAQMQADGADTFIEFGPKAVLTGLVKKTLKGVTAKNCESVDTLKEVMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5V956</accession>
    <name>R5V956_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00492</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000257">
        <property type="protein sequence ID" value="CCZ84639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00492"/>
    <dbReference type="EnsemblBacteria" id="CCZ84639">
        <property type="protein sequence ID" value="CCZ84639"/>
        <property type="gene ID" value="BN566_00492"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="172" mass="20230" checksum="E73BA98523540A01" modified="2013-07-24" version="1">MLKAIGSRKNLKQQLYYEFVADLLESKSVQDLKQYRHHIKTTRFQHSLNVSYYNYLICKFFHWDAVSAARAGLLHDLYFYETADYDATETHESHSAHHPQVALHNAMQLFSVNAVEKDIIQNHMWPATHHMPHYKESYVIVVVDKYCAALEYVAPSLRSLFRKVFRRKRAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5VGR3</accession>
    <name>R5VGR3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Zn-dependent hydrolases including glyoxylases</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00576</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000007">
        <property type="protein sequence ID" value="CCZ82771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00576"/>
    <dbReference type="EnsemblBacteria" id="CCZ82771">
        <property type="protein sequence ID" value="CCZ82771"/>
        <property type="gene ID" value="BN566_00576"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="13"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="207" mass="22781" checksum="13BBE7CCEF72E38A" modified="2013-07-24" version="1">MAKITRIMVAPLAVNCYIIATEQHNAVAIDAGGSVPKILQFLQDNGLTLQKILLTHGHYDHIGGVAELQAATGAEVYIHEADAPMLSDSNLNLEKWITGETETTPISEYHAIHDGDTIVQDECVFHVLHTPGHTKGGVCYQLDRTLFTGDTLFRCSRGRTDFPGGSDAELYASLQRLKQLEGDYDVLPGHNETSTLSFEREHNPNLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5VHF4</accession>
    <name>R5VHF4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Serine/threonine protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00766</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000032">
        <property type="protein sequence ID" value="CCZ82966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00766"/>
    <dbReference type="EnsemblBacteria" id="CCZ82966">
        <property type="protein sequence ID" value="CCZ82966"/>
        <property type="gene ID" value="BN566_00766"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032485">
        <property type="entry name" value="DUF5050"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16472">
        <property type="entry name" value="DUF5050"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="DUF5050" evidence="2">
        <location>
            <begin position="208"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="DUF5050" evidence="2">
        <location>
            <begin position="322"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ82966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="420" mass="47512" checksum="2FA1D685697976A1" modified="2013-07-24" version="1">MDRKFCRYCARILGEDGKCHTKNCPSNTQKRKNGIALYCPACGRVLINGACKNKACPSYISAMAAAKTDVAKNVVVEERQKSKQVRNLKKRKQVIISLASLVAASSIIGGTILYWNANHHQTDLTSSTANANIGIIQKNTRATTTTSVFTTTATTVEETTAPPETTTVATTTTTEVADFGSTNLINGGYVWYDGDEYRVADNFYSNITIGEKLYYEEASTHYVYCCNRKDLSDKTLIYNAAYVDELTYYDGWFYFRRTLGDNVSICKMRTDGSDVTVLTQCNEWYMNVYQDKIYFVNFDNNYALQCMDLNGNQLVTLTYGASDICVVKDTIFFSNRDTRYLYKMNLDGTNLVQLNQTYTRCTNYYDGKLYYFGDNDHMIYRCDLMGNVEKSYESGANFLVLAGGYIYAYMDNAQIRKIAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5VHV3</accession>
    <name>R5VHV3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00888</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000059">
        <property type="protein sequence ID" value="CCZ83101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00888"/>
    <dbReference type="EnsemblBacteria" id="CCZ83101">
        <property type="protein sequence ID" value="CCZ83101"/>
        <property type="gene ID" value="BN566_00888"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024234">
        <property type="entry name" value="DUF3801"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12687">
        <property type="entry name" value="DUF3801"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="158" mass="18355" checksum="808E4022D651C63B" modified="2013-07-24" version="1">MPSDYENGAKKTIDISIKAEKMTADVLKSALQEFMSGKAEKKGRMTYKQLQAKSPSKLDSIEVSDRNIGDFLKTARKYDVDFALKRDKSTSPPTYHVFFSAAKTEDFKRAFSEYVGKDQCKTPKRGEFTREKMQQQAQKLRSKPRKQKQREKTRENRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5VIA7</accession>
    <name>R5VIA7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01077</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83296">
        <property type="protein sequence ID" value="CCZ83296"/>
        <property type="gene ID" value="BN566_01077"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005240">
        <property type="entry name" value="DUF389"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20992">
        <property type="entry name" value="PTHR20992"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04087">
        <property type="entry name" value="DUF389"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="138"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="199"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="243"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="261" mass="28687" checksum="C168E175A51A88B7" modified="2013-07-24" version="1">MNRYEMDKERENFRTILKKMFSITEDAASNEEIRDRILSGGKITGTNMVVMICAILIASVGLNTNSIAVIIGAMLISPLMGSILAISYGTVSNDAYLIKRHSFGFAVQIVMSVVTATIYFLLSPVKEPTEQLLARTNPNFFDVIIAISGGVAGIVGQTRKDKSNNIIPGVAIATALMPPLCTCGYALANGEFRMLLGAFYLFIINAYFIYIASDVMLTLLGTPRIKAMSAAEWKRARKKMHRNTILVLLPIILIAIGWNIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJ58</accession>
    <name>R5VJ58_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AAA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01290</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000121">
        <property type="protein sequence ID" value="CCZ83521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01290"/>
    <dbReference type="EnsemblBacteria" id="CCZ83521">
        <property type="protein sequence ID" value="CCZ83521"/>
        <property type="gene ID" value="BN566_01290"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008533">
        <property type="entry name" value="DUF815"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05673">
        <property type="entry name" value="DUF815"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="201"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="405" mass="45410" checksum="1FC4FA9C270C4479" modified="2013-07-24" version="1">MTLKEELGYGLDALVLFRGLQQNPVIHALMEFCHADSSDLANQIRTYSSIVAALYPATDNLTDYMEKIVLEEETVYVQRIGAGEAVSDAMKKCLETELHLLQKIAMLTSSQLQAEMPYFPDLPTWNNRKIDLAAAYHKRVADIGKHGYGVYAQYHMFTLDETGDIIPICYPDPIDLDHMVGYEQERNIVIDNTKALLNGKYAANVLLAGDAGTGKSATVKAIANAYREEGLRLIELTKDQLRFIPRIMEHLCRNPLKFILFIDDLTFSENDPNFGTLKATLEGSIAARMKNTVIYATSNRRHLVKETFDDGSDKHQNDTVQERISLSERFGITVTYTRPQKELYLEIVHSLAEQAGLHMDASQLDMEAEQFALGKCGRSARAARQFIDTLLTKEPTVTEGAESSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJE0</accession>
    <name>R5VJE0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01377</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000147">
        <property type="protein sequence ID" value="CCZ83641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83641">
        <property type="protein sequence ID" value="CCZ83641"/>
        <property type="gene ID" value="BN566_01377"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="73" mass="8628" checksum="2F986874170BB052" modified="2013-07-24" version="1">MKKELEFLYEGNCNENPFVFETPELQEQMDSVFSYFQKRHAEKGQEALCELCENWQRNAFVVGFQTAVNLLTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJP7</accession>
    <name>R5VJP7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 7">3-dehydroquinate dehydratase</fullName>
            <shortName evidence="7">3-dehydroquinase</shortName>
            <ecNumber evidence="5 7">4.2.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Type II DHQase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">aroQ</name>
        <name evidence="11" type="ORF">BN566_01506</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes a trans-dehydration via an enolate intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>3-dehydroquinate = 3-dehydroshikimate + H2O</text>
            <dbReference type="Rhea" id="RHEA:21096"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16630"/>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
            <dbReference type="EC" id="4.2.1.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 7">Homododecamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the type-II 3-dehydroquinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.10" evidence="5 7"/>
    <dbReference type="EMBL" id="CBAR010000161">
        <property type="protein sequence ID" value="CCZ83771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01506"/>
    <dbReference type="EnsemblBacteria" id="CCZ83771">
        <property type="protein sequence ID" value="CCZ83771"/>
        <property type="gene ID" value="BN566_01506"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00086"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003855">
        <property type="term" value="F:3-dehydroquinate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00466">
        <property type="entry name" value="DHQase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.9100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00169">
        <property type="entry name" value="AroQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001874">
        <property type="entry name" value="DHquinase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018509">
        <property type="entry name" value="DHquinase_II_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036441">
        <property type="entry name" value="DHquinase_II_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21272">
        <property type="entry name" value="PTHR21272"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01220">
        <property type="entry name" value="DHquinase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001399">
        <property type="entry name" value="DHquinase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52304">
        <property type="entry name" value="SSF52304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01088">
        <property type="entry name" value="aroQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01029">
        <property type="entry name" value="DEHYDROQUINASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="7 9">
        <location>
            <begin position="101"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7 8">
        <location>
            <position position="23"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7 8">
        <location>
            <position position="100"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="7 10">
        <location>
            <position position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001399-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001399-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001399-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ83771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="142" mass="15518" checksum="0CC1A313A09B8BC0" modified="2013-07-24" version="1">MKNVLVIHGPNLNLLGERDPAFYGNVTFDKLNEMILERAEALGLHCEIFQSNHEGAIIDKLQAARKTMDGVVINAGAYTHYSYAIHDAIADMQLPCVEVHISNIQARDAFRSKSVIAPVCTGTIAGFGINSYFLALQALAML</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5VK99</accession>
    <name>R5VK99_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Coenzyme F420-reducing hydrogenase delta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01680</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000175">
        <property type="protein sequence ID" value="CCZ83951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01680"/>
    <dbReference type="EnsemblBacteria" id="CCZ83951">
        <property type="protein sequence ID" value="CCZ83951"/>
        <property type="gene ID" value="BN566_01680"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003813">
        <property type="entry name" value="MvhD/FlpD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02662">
        <property type="entry name" value="FlpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FlpD" evidence="2">
        <location>
            <begin position="11"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="147" mass="16832" checksum="147776CEDD005C92" modified="2013-07-24" version="1">MQVNNEFKPKIVAFCCNWCSYAGADLAGTNRANYPADVKIIRVPCSCRVNPIFILRAFQRGADGVILCGCHPGDCHYTTGNYYARRRMTMLFSMLNYLGIEKERTRVEWVSAAEGAKFAETMNEFTETILKLGENKRLEDLRCKKEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>R5VKJ9</accession>
    <name>R5VKJ9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ABC-type transport system involved in lipoprotein release permease component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN566_01740</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000178">
        <property type="protein sequence ID" value="CCZ84011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01740"/>
    <dbReference type="EnsemblBacteria" id="CCZ84011">
        <property type="protein sequence ID" value="CCZ84011"/>
        <property type="gene ID" value="BN566_01740"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004407189" evidence="7">
        <location>
            <begin position="35"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="301"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="349"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="395"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX" evidence="8">
        <location>
            <begin position="330"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ84011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="431" mass="48042" checksum="A0FFD6320E95F6CF" modified="2013-07-24" version="1">MAFRNVFREKKRAVLVFASLFLGTMAFLSTNAFVDSMKLENYVKYYLPNDYTIYVNCGSEQEGTEEEQIQKNIKSAEEMAQKIQALDGITDVSIHRYEDVTLPFDETLFQPFLESFLESISEKMTLEEFAKGYTDSPESYTSTVIVISKKDVETYNQKAKTPIDIDAFLAGTQCIIGSVNTPEQAAQMQGKTITMKDKNGNALSLQVGSCALNGDISGLSFGQYWRLAGAPEYILVSEAALSQLTDTPAINTIAIQCDPKAESRLTNQIRSITQMYPIVAHLEIRTELMQEFQDSMSSLKILSGGVSAILILIGIINFISVMLTGIFTRRTELAVMESVGMTKKQVQKMLMLEGIYYAVITLALILTAGSGILYLVGFFTEQTADYAVFQYPWGWIAILACFIFAICVFVPMLVYRIATKESITARLRSID</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5VKL2</accession>
    <name>R5VKL2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Precorrin-8X methylmutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_01752</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CobH/CbiC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000180">
        <property type="protein sequence ID" value="CCZ84026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01752"/>
    <dbReference type="EnsemblBacteria" id="CCZ84026">
        <property type="protein sequence ID" value="CCZ84026"/>
        <property type="gene ID" value="BN566_01752"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016993">
        <property type="term" value="F:precorrin-8X methylmutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003722">
        <property type="entry name" value="Cbl_synth_CobH/CbiC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036588">
        <property type="entry name" value="CobH/CbiC_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02570">
        <property type="entry name" value="CbiC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63965">
        <property type="entry name" value="SSF63965"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CbiC" evidence="5">
        <location>
            <begin position="12"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ84026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="211" mass="22791" checksum="904D852C462BC39A" modified="2013-07-24" version="1">MEKKIILEQPMEIEKKSFSIIEEHLQGVTLPDAERSILKRVIHTTADFDYIQNLVFSETAVSTALEALQQGADIVTDTNMALSGINKRALQKLGGTAHCFMADEEVAAIAKQKGVTRAMVSMEKACSLPKDCIFAIGNAPTALIRLSELIAEQKIQPKLIIGVPVGFVNVIEAKELILAGTTPYIVAKGNKGGSNVAAAICNALLYQLIQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5VKN4</accession>
    <name>R5VKN4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">LPxTG motif-containing cell surface protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01792</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000184">
        <property type="protein sequence ID" value="CCZ84066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84066">
        <property type="protein sequence ID" value="CCZ84066"/>
        <property type="gene ID" value="BN566_01792"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00222">
        <property type="entry name" value="CollagenBindB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008454">
        <property type="entry name" value="Collagen-bd_Cna-like_B-typ_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05738">
        <property type="entry name" value="Cna_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="191"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="CNA-B" evidence="2">
        <location>
            <begin position="62"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="218" mass="24911" checksum="E0042C5E55C6DD41" modified="2013-07-24" version="1">MTEEITDATGTVLYKPCITTADGSVWEHTITGIIQESKAQQSENWITIKNYQADAVPEQKDITVQKQWDDSIQHPDITVLLYQMQTNRNTGTSTTILYDTKTLNDANQWAYTWSNVPRSDKTNDYSYYIQEKAVDGYDPSYYTDKIQLLLTKCVVDDTEVSVAPVDWEKASVTIFNRKRPELPKTGGRGTIWYLMSGFLLFLFAGFGISMQMRKIKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5VKT7</accession>
    <name>R5VKT7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Metal dependent phosphohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01861</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000192">
        <property type="protein sequence ID" value="CCZ84136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01861"/>
    <dbReference type="EnsemblBacteria" id="CCZ84136">
        <property type="protein sequence ID" value="CCZ84136"/>
        <property type="gene ID" value="BN566_01861"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="169" mass="19092" checksum="9EC76908D22F5C60" modified="2013-07-24" version="1">MDAKINELVTAMITLYHDDPKRIHHFMKVYAFASAIAQSEQVNALLQTRIEAAALVHDIGIHAAERKYRSAAGAYQEKEGPPLARAMLMTIGFSDSLIDRVCFLVGHHHTYTEIDGIDFQILVEADFLVNAREEKMTKKAITHFRNVYFQTETGKQYLDETYGLNSLSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>R5VL06</accession>
    <name>R5VL06_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Energy-coupling factor transporter ATP-binding protein EcfA2</fullName>
            <ecNumber evidence="6">3.6.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_01946</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">ATP-binding (A) component of a common energy-coupling factor (ECF) ABC-transporter complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Forms a stable energy-coupling factor (ECF) transporter complex composed of 2 membrane-embedded substrate-binding proteins (S component), 2 ATP-binding proteins (A component) and 2 transmembrane proteins (T component).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 6">Cell membrane</location>
            <topology evidence="2 6">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ABC transporter superfamily. Energy-coupling factor EcfA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.-" evidence="6"/>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01946"/>
    <dbReference type="EnsemblBacteria" id="CCZ84221">
        <property type="protein sequence ID" value="CCZ84221"/>
        <property type="gene ID" value="BN566_01946"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03225">
        <property type="entry name" value="ABC_cobalt_CbiO_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015856">
        <property type="entry name" value="ABC_transpr_CbiO/EcfA_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030946">
        <property type="entry name" value="EcfA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04521">
        <property type="entry name" value="ECF_ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="7">
        <location>
            <begin position="4"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="41"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ84221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="290" mass="32488" checksum="9156FF2A2CA01033" modified="2013-07-24" version="1">MAILKTEDLTYQYSIGTPFEKTAVDHVNLEIEEGAFVGIIGHTGSGKSTLIQHFNGLIRPTSGKIYLDGTDIWADKTNIRQVRFQVGLVFQYPEYQIFEDTVYKDIAFGPRNMGLSEAEIKERVEETAALVGLTQAQLNQSPFDLSGGQKRRVAIAGVMALRPKVLILDEPTAGLDPKGREDILREIRRYHKETGRTVLLVSHSMEDMANCAEKILVMNAGKVFCYDTVENVFRQAKALQEIGLAVPQITRVCMQLREQGIPLSDDIYTVEAAYQQILQLYRQQKEGTGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5VL48</accession>
    <name>R5VL48_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00200</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000204">
        <property type="protein sequence ID" value="CCZ84271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00200"/>
    <dbReference type="EnsemblBacteria" id="CCZ84271">
        <property type="protein sequence ID" value="CCZ84271"/>
        <property type="gene ID" value="BN566_00200"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019660">
        <property type="entry name" value="Put_sensory_transdc_reg_YbjN"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10722">
        <property type="entry name" value="YbjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="172" mass="19832" checksum="ACE5AC4F8E08E2C8" modified="2013-07-24" version="1">MLDGILTYFYLKGESIMARKFSLEIANAIENFLSKDDWHYQPCNDNGVIRMGITLNCKFKRADIYIGIDDDAFRILTVFPMTVDEDVRKDMAEFLTRANYGLRPCNFEMDFDDGEIRCKSFHNCADTIPTDQQIQDALYYNIKIIERYGNGIAKIMFGIGTPTEVLKECENS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5VLW9</accession>
    <name>R5VLW9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CAAX amino terminal protease family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00052</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000241">
        <property type="protein sequence ID" value="CCZ84506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84506">
        <property type="protein sequence ID" value="CCZ84506"/>
        <property type="gene ID" value="BN566_00052"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071586">
        <property type="term" value="P:CAAX-box protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="297"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="339"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="366" mass="40131" checksum="0A4FF368DD0019D5" modified="2013-07-24" version="1">MNPTLRPDPYDPFSNPTQHRAYASHYRTILPQIGLPGYQVPLKPTKQERKRIRHYFNAAGFGLTAHFFLVQILALVVMAVLQGIMGFAKNGVDANAYIQQSSILMAVNGLLFLMANTSVAAIGCRATKIPIRNLFHTNTFRFSEILPYLFIGIFIQSASGDVSSWITDLLGQGGITAYEPDIDYYGTGTAVVAEILYGCLIAPITEEFLYRGFVLKNLSRVSQRTGILLSAFLFGLGHQNISQFLLAFPLGVFLGALAVRHNSILPSIFVHVTVNSAATIFGMGYERITDPEAASMFAVYGNILYTLLAVIGAILLVLHLRKHIFPKNTPAQSLRGGRILCTAPWLLVIILLNLAVAIEAIWSASV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5VLX3</accession>
    <name>R5VLX3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5 7">50S ribosomal protein L3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rplC</name>
        <name evidence="8" type="ORF">BN566_00057</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 7">One of the primary rRNA binding proteins, it binds directly near the 3'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 7">Part of the 50S ribosomal subunit. Forms a cluster with proteins L14 and L19.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the universal ribosomal protein uL3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000241">
        <property type="protein sequence ID" value="CCZ84511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00057"/>
    <dbReference type="EnsemblBacteria" id="CCZ84511">
        <property type="protein sequence ID" value="CCZ84511"/>
        <property type="gene ID" value="BN566_00057"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01325_B">
        <property type="entry name" value="Ribosomal_L3_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000597">
        <property type="entry name" value="Ribosomal_L3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019927">
        <property type="entry name" value="Ribosomal_L3_bac/org-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019926">
        <property type="entry name" value="Ribosomal_L3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11229">
        <property type="entry name" value="PTHR11229"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00297">
        <property type="entry name" value="Ribosomal_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03625">
        <property type="entry name" value="L3_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00474">
        <property type="entry name" value="RIBOSOMAL_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 7" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ84511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="210" mass="22094" checksum="1620EFA41205D019" modified="2013-07-24" version="1">MQKAIIGKKIGMTQIFDENGKVIPVTVVEAGPCTIVQKKTVENDGYAAVQMGYGDVKPQRLNKPEKGHFAKADVAPKKTLKEFRLDNSDALNVGDVVKADTFAVGDSVDVSGTSKGKGFAGAIKRHNQHRLKETHGTGPVHRQAGSMGACSSPSRIYKGKGMPGHMGAEAVTVQNLEIVKIDVENNLIAVKGAIPGPKGGIVAIVDSVKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5VM63</accession>
    <name>R5VM63_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted Zn-dependent protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00484</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000257">
        <property type="protein sequence ID" value="CCZ84631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00484"/>
    <dbReference type="EnsemblBacteria" id="CCZ84631">
        <property type="protein sequence ID" value="CCZ84631"/>
        <property type="gene ID" value="BN566_00484"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007395">
        <property type="entry name" value="Zn_peptidase_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36434">
        <property type="entry name" value="PTHR36434"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04298">
        <property type="entry name" value="Zn_peptidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="191"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="222" mass="24461" checksum="74E61D3C4B440199" modified="2013-07-24" version="1">MGYGLLLIGFLICAIASWNVNATFKRYQKISNRRGMTAAEAARQILNENGLQFIQIERVSGSLTDHYDPRANVIRLSDSVYDSTSVAAIGVAAHECGHAVQHAVGYTPIKIRQAIIPVTQFGSYAWYFVLVLGLILSSRPLVYGGILLFSLIVLFQFVTLPVEFDASHRALKTLDAYHMLDDDEIGSARKVLTAAAMTYVASLVSSILQLVRLLGLVDRRKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>R5VMA7</accession>
    <name>R5VMA7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">Argininosuccinate synthase</fullName>
            <ecNumber evidence="2 9">6.3.4.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Citrulline--aspartate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">argG</name>
        <name evidence="10" type="ORF">BN566_00534</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + L-aspartate + L-citrulline = 2-(N(omega)-L-arginino)succinate + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10932"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57472"/>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the argininosuccinate synthase family. Type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.5" evidence="2 9"/>
    <dbReference type="EMBL" id="CBAR010000267">
        <property type="protein sequence ID" value="CCZ84681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00534"/>
    <dbReference type="EnsemblBacteria" id="CCZ84681">
        <property type="protein sequence ID" value="CCZ84681"/>
        <property type="gene ID" value="BN566_00534"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00113"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004055">
        <property type="term" value="F:argininosuccinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01999">
        <property type="entry name" value="Argininosuccinate_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00005">
        <property type="entry name" value="Arg_succ_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001518">
        <property type="entry name" value="Arginosuc_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018223">
        <property type="entry name" value="Arginosuc_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023434">
        <property type="entry name" value="Arginosuc_synth_type_1_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024074">
        <property type="entry name" value="AS_cat/multimer_dom_body"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11587">
        <property type="entry name" value="PTHR11587"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00764">
        <property type="entry name" value="Arginosuc_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69864">
        <property type="entry name" value="SSF69864"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00032">
        <property type="entry name" value="argG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00564">
        <property type="entry name" value="ARGININOSUCCIN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00565">
        <property type="entry name" value="ARGININOSUCCIN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3 9" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="11"/>
            <end position="19"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ84681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="408" mass="45257" checksum="D03FEFB05B6263F6" modified="2013-07-24" version="1">MAKEIKKVVLAYSGGLDTSIIIPWLKENYNNCEVIAVSADVGQGTELDGLEEKAIKTGASKLIVLDLKDEFVNDYVFPTVQAGAVYENRYLLGTSFARPIIGKKLAEIALEEGADAICHGATGKGNDQVRFELAIKAYAPDMKIIAPWREWSIKSRDEEIDYAEAHNIPLKINRETNYSKDKNLWHLSHEGLDLEDPANEPQYEKKGFLELGVSPIDAPDKPTYVTIHFEKGIPTAIDGKKMGGVEIVETLNKLGGENGIGLADLVENRLVGMKSRGVYETPGGTILYHAHDVLETITLDKETARVKQYLSIKFADIVYNGQWYTPLREALTAFVNETQKTVTGDVKLKLYKGNIINAGVTSPYTLYDEEVATFDAEDVYNQKDAEGFINLFGLPIHVKAKLDQKRGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5VQ93</accession>
    <name>R5VQ93_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00007</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000001">
        <property type="protein sequence ID" value="CCZ82752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00007"/>
    <dbReference type="EnsemblBacteria" id="CCZ82752">
        <property type="protein sequence ID" value="CCZ82752"/>
        <property type="gene ID" value="BN566_00007"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="SSF81653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="222" mass="23758" checksum="2FCEF9501AA6E790" modified="2013-07-24" version="1">MKRIMEKLETLLEWGGLKKEIILLVISGISLLLSLFHIPLPFSIAWVAIILCGVPIILEAIIGLVTAFDIKADVLVSLALVASICIGEHFAAGEIAFIMQLGALLEDLTVAKARAGIEKLVHLTPQTARVLRNGTEKIIPAEQVQVQDLLRVLPGETIPVDGVITDGQTSINQAVMTGESLPVDKTVGDTVSSGTVNQFGAFEMRATKVGADSSIQRMIQLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VRC0</accession>
    <name>R5VRC0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00889</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000059">
        <property type="protein sequence ID" value="CCZ83102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83102">
        <property type="protein sequence ID" value="CCZ83102"/>
        <property type="gene ID" value="BN566_00889"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="92" mass="10298" checksum="BEEC2EA667941F7A" modified="2013-07-24" version="1">MAKSILTPEGDLVNYDNLIAVSVEVRPVGVDEEHSEDEYCIVGTDVTNKENLLYHSPDYDKVMSVQRDITRWLQSEAFSTFEMPTADEGGDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>R5VRJ7</accession>
    <name>R5VRJ7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">RNA polymerase sigma factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_00965</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000069">
        <property type="protein sequence ID" value="CCZ83182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83182">
        <property type="protein sequence ID" value="CCZ83182"/>
        <property type="gene ID" value="BN566_00965"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="3">
        <location>
            <begin position="11"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="4">
        <location>
            <begin position="103"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="177" mass="20989" checksum="5C94749C5E6F80B5" modified="2013-07-24" version="1">MDKSTFQQKLQQYKDMVYRIAYTYLRNTADAEDVSQETFLKFYQLSKPFTDAGLEKAWLIRVTLNACHNLRKSVWYNRRADYKEIPELFSDPTDNLLYEEVFALPERYRIVILLYYYEEYSIQEIADLTGRKISTIQTQLARARKKLKLALTEQGRDFYEQESLSANYESHSNAGTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5VRN3</accession>
    <name>R5VRN3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01000</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000074">
        <property type="protein sequence ID" value="CCZ83217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83217">
        <property type="protein sequence ID" value="CCZ83217"/>
        <property type="gene ID" value="BN566_01000"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004395589" evidence="2">
        <location>
            <begin position="30"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="102" mass="10558" checksum="E7C26C989CD3E85E" modified="2013-07-24" version="1">MHARKWLPALLAVACLTGCGGKTNDSSQPATTTTITNSPGDSIVTKLSDVADDIGDAGKDVLTDIGDAAEDVKDDLMGETTETHTTISDSLESTKSTETTTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5VRS8</accession>
    <name>R5VRS8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01048</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01048"/>
    <dbReference type="EnsemblBacteria" id="CCZ83267">
        <property type="protein sequence ID" value="CCZ83267"/>
        <property type="gene ID" value="BN566_01048"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="191"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="232"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="265"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="298"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="356"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="387" mass="43148" checksum="23D36884F63864CA" modified="2013-07-24" version="1">MQVFKAFLRILRKKLLTAMIWVVVFLIIAVGVTTNNSSPFSFTENQFNICVFDEDQTPESQALVSYLGKHHNLVSVKQEQDTILDMLYDERIDYAMTIAKGYAENLQSGKTDTLFTHYYLDDRYANTLLDSTLSEYVKTVLAYETSGLSCMDAISSAEAVLSEEISVNSDPFAETANPASHNESFSYYFQYLPYIFLSVLIAALSPTLIALQKQDIRNRTNCSCLSSSSQTLQMLLGSGLFVLFVWLIFMVAALWFNGGMVTGKLWYAILNSFIFLLVAAAIAILLSAAAPSDTVLNVWNQVIGLGMSFLCGIFVPQSMLSDGVLKAGQFLPAYWYVRANNMLFGISGEVFQTRTFLQYLGVECLFAVALFALIFAVQKAKHDRSNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-09-29" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5VRZ2</accession>
    <name>R5VRZ2_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Resolvase/invertase-type recombinase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01108</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000086">
        <property type="protein sequence ID" value="CCZ83327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83327">
        <property type="protein sequence ID" value="CCZ83327"/>
        <property type="gene ID" value="BN566_01108"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00857">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="SSF53041"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="1">
        <location>
            <begin position="3"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="104" mass="12072" checksum="375161357F6C547C" modified="2013-07-24" version="1">MEKIIGYIRYSSHAQDDGNSVAAQTTCIEQYATAHDMEIEKFYMDTAKSGRFTENRPEYNRMKEEIQNGTVQAKTIVVRALDRLHRNAAHQLEDLTFLKNRTFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5VS68</accession>
    <name>R5VS68_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01163</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000094">
        <property type="protein sequence ID" value="CCZ83387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01163"/>
    <dbReference type="EnsemblBacteria" id="CCZ83387">
        <property type="protein sequence ID" value="CCZ83387"/>
        <property type="gene ID" value="BN566_01163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018580">
        <property type="entry name" value="Uncharacterised_YfhO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38454">
        <property type="entry name" value="PTHR38454"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09586">
        <property type="entry name" value="YfhO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="188"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="228"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="288"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="311"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="339"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="367"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="393"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="425"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="841"/>
            <end position="859"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="870" mass="99705" checksum="854EE54B0B761D73" modified="2013-07-24" version="1">MQRIQQKKHGSIRDFYAAAILTGCLTIFLCLLWVIHPDLIFGSTMDWNNQHIIFPDYFRSRFYQTGQLFPNFASAIGAGQNIYEFTYYGLFNPIFLLSYLLPFVPMMDYIQCSSILLIWGSGMLCYWFCRQHFSERIAFVCGFLYLFSAPLIYHGHRHLMFMNYLPFLFWALFAVRRCQTHHHVSISLILASVCILGCSFFFAVGSFLTIFLYAWFLYLQESGLKKSFLLRILVHLFFAGMLCMCFLLPTALALLQGRSETHLDKKFWLEVFVPTVQLSYFFYQPYGVGLTITGLFALLVGCWTKKKAVRFLSVTLLLLLCLPMLLYLINGFQYLDGKAYIPLLPLAILVYGFFFQSLQTKQIPWNTILPLFVLLLASGFFFAWNMLHGSKRWILLGGATAETILFLCLLQPWRTPKMPNNWKRILWMACSMQLVYSFLLNGTDYTLLKKSTMQQYQNADIDTLWKQTTEQDDSWYRVYNATQPYATANQTQQGTCFLSTVYASVQNKTYSNFYYHQLFNETSIRNNAAVDAVKNLAYHIWMGDKYRLDFKGKTLFGYEPKYTSGDYCVYENQYALPIGYATDRWISMDTFQQHNEAEQVLDLLSGIVLPEQVADTEMEQPKSLQTISEQEIESVLQTIEQEFPVTRNGDTLLISTEQPMTKTISMPKSCAGKLLLLSMEVDNTVVANSDDVFIMINGIKNKLTAPSWKYHNQNYHFTYLLSDVTDTLELSFSAGTYQVSNLHGYTLPETVLEQAVQQVTPLETTAEQVRGDTISGTITTEKDGWFVLSVPYDTGFTVTVDGTETAYEKVNLSFLGIPLSAGTHEIVWTYHAPGAATGKKITLITAGILLLWVGLSYGIQRKQSERRILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>R5VSB9</accession>
    <name>R5VSB9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Sialate O-acetylesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01201</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000102">
        <property type="protein sequence ID" value="CCZ83427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01201"/>
    <dbReference type="EnsemblBacteria" id="CCZ83427">
        <property type="protein sequence ID" value="CCZ83427"/>
        <property type="gene ID" value="BN566_01201"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001681">
        <property type="term" value="F:sialate O-acetylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005181">
        <property type="entry name" value="SASA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039329">
        <property type="entry name" value="SIAE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22901">
        <property type="entry name" value="PTHR22901"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03629">
        <property type="entry name" value="SASA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="2" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SASA" evidence="3">
        <location>
            <begin position="84"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="SASA" evidence="3">
        <location>
            <begin position="272"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="509" mass="57064" checksum="DD18D393508CD48F" modified="2013-07-24" version="1">MSLKLAAIFKDFMVVQRNRPITVFGTGVPKTVVTVSLHTSFSKTVVAADGTWCATLPPLAAGTDYVLTVSDGTTEEQRKQVAVGEVWLAGGQSNMELALRDSADGVAVSKAYTGTQIRFYQVPKRAVLDETHQQLEAQSAWKIAAAENVGDLSAVAFYFAKRLAQKMDCVIGIVDCYWGGTSIACWMSESMMQSVAAGQKQLADYKAEVGAKTPEQYAAEMREYEADYQKWQANIDACRAENPNVTWKELHERFGECPWPQPTGWQSPFCPNNLHRTMMQRVAPYTLRGFLYYQGEEDVSRYEGYSDMMIALVKQWRMDWNDWSLPFLFVQLPMYLAIDTEDDKKWAMQREQQAIAAAMLKRVGMISMTDCGEFDNIHPLDKETPGTRLALLAEQMVYGTGEEWTPTVQQMYSDGTALYLVFDHVADGLVIHASQPETCQIAGADGVYYPAKAEVLHSNTIRVSADAVDCPQSVQYAWYNYGEATWFTQSRIPVPSFRYALASREGQTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5VSI0</accession>
    <name>R5VSI0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphotransferase System HPr (HPr) Family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01251</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000116">
        <property type="protein sequence ID" value="CCZ83482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01251"/>
    <dbReference type="EnsemblBacteria" id="CCZ83482">
        <property type="protein sequence ID" value="CCZ83482"/>
        <property type="gene ID" value="BN566_01251"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00367">
        <property type="entry name" value="PTS-HPr_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1340.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000032">
        <property type="entry name" value="HPr-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035895">
        <property type="entry name" value="HPr-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00381">
        <property type="entry name" value="PTS-HPr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00107">
        <property type="entry name" value="PHOSPHOCPHPR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55594">
        <property type="entry name" value="SSF55594"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01003">
        <property type="entry name" value="PTS_HPr_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51350">
        <property type="entry name" value="PTS_HPR_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HPr" evidence="1">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="85" mass="9465" checksum="41C8136B154A9F34" modified="2013-07-24" version="1">MKEFCYTIKNAVGVHARPAGALARMAEKYDSTILIEKDGTAVNLEKLLAVMKLNIQYQETVKVTIEGSDEAAALEQIQTFFEENL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>R5VSM4</accession>
    <name>R5VSM4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phosphoglycolate phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01286</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000119">
        <property type="protein sequence ID" value="CCZ83517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01286"/>
    <dbReference type="EnsemblBacteria" id="CCZ83517">
        <property type="protein sequence ID" value="CCZ83517"/>
        <property type="gene ID" value="BN566_01286"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006549">
        <property type="entry name" value="HAD-SF_hydro_IIIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01662">
        <property type="entry name" value="HAD-SF-IIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="216" mass="24149" checksum="08F330C7E9A5BC8E" modified="2013-07-24" version="1">MRYQLAIFDMDGTILNTLQDLTNSLNAALQLSGYPQHTLEEVRFFVGNGIRKLIERGVPAGTPVAQIDKVHQDFTEHYRVHCADTTQPYAGIPELLQQLRNAGCKTAVVSNKADYAVQELCKQYFDGMFDFAVGERKNILKKPAPDAVNEVLQQLQTDRSQAVYIGDSDVDVETAKNAKMDGIFVDWGFRSRDFLLEHGAKTIVSQPEELAEKILG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5VTA2</accession>
    <name>R5VTA2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative Xaa-Pro dipeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_01507</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000161">
        <property type="protein sequence ID" value="CCZ83772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01507"/>
    <dbReference type="EnsemblBacteria" id="CCZ83772">
        <property type="protein sequence ID" value="CCZ83772"/>
        <property type="gene ID" value="BN566_01507"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008235">
        <property type="term" value="F:metalloexopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_NTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000587">
        <property type="entry name" value="Creatinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001714">
        <property type="entry name" value="Pept_M24_MAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001131">
        <property type="entry name" value="Peptidase_M24B_aminopep-P_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01321">
        <property type="entry name" value="Creatinase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00599">
        <property type="entry name" value="MAPEPTIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53092">
        <property type="entry name" value="SSF53092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="SSF55920"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00491">
        <property type="entry name" value="PROLINE_PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Creatinase_N" evidence="4">
        <location>
            <begin position="3"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M24" evidence="3">
        <location>
            <begin position="137"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="359" mass="39338" checksum="7DC120CBD99D1F97" modified="2013-07-24" version="1">MTRIEQLMQRLPEGIDCAWISADVNRRYFTGMASSAGNVICFRDGAYLLIDFRYIEKARACVKDCQVIELENLSVQVENLLQQHHATTIAVESAEMTLQQFSNLQKRFSHQYTWDTSNVLSDAIKECRMVKTPDEIEQIQAAQRIAEAALQNVIDWLHVGVTEREIQRKLDFTMLELGAEAMSFPTIALTGARTSMPHGVPSDAAVQAGDFVLMDFGAMVNGYHSDMTRTVCVGTPTEKMKSVYEVVRQAQAAALQAARAGITGKALDAIARDIITAAGYGKQFGHSLGHGVGMDIHEYPNAAPSSHVSLEPGNVVTIEPGIYLPGEFGVRIEDFVAITETGCRNLTAVTPDLICIPAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VTQ5</accession>
    <name>R5VTQ5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01631</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000171">
        <property type="protein sequence ID" value="CCZ83902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83902">
        <property type="protein sequence ID" value="CCZ83902"/>
        <property type="gene ID" value="BN566_01631"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="135" mass="15540" checksum="F5080F1CF2E936C6" modified="2013-07-24" version="1">MIIQMVLCQICKDRSPEVGRANAVLHQTERRNFHNYIITASIRHFPIQCHQIIHHWCGIVSMQNLVTDFILNRANQADQITICGQNVFNHICGGRLSVCTRNTDEGHFPFRMSEPSCTELSIENIGVWHKNLLFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>R5VTZ1</accession>
    <name>R5VTZ1_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">30S ribosomal protein S2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpsB</name>
        <name evidence="7" type="ORF">BN566_01716</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the universal ribosomal protein uS2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000178">
        <property type="protein sequence ID" value="CCZ83987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01716"/>
    <dbReference type="EnsemblBacteria" id="CCZ83987">
        <property type="protein sequence ID" value="CCZ83987"/>
        <property type="gene ID" value="BN566_01716"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01425">
        <property type="entry name" value="RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00291_B">
        <property type="entry name" value="Ribosomal_S2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001865">
        <property type="entry name" value="Ribosomal_S2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005706">
        <property type="entry name" value="Ribosomal_S2_bac/mit/plastid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018130">
        <property type="entry name" value="Ribosomal_S2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023591">
        <property type="entry name" value="Ribosomal_S2_flav_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12534">
        <property type="entry name" value="PTHR12534"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00318">
        <property type="entry name" value="Ribosomal_S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00395">
        <property type="entry name" value="RIBOSOMALS2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52313">
        <property type="entry name" value="SSF52313"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01011">
        <property type="entry name" value="rpsB_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00962">
        <property type="entry name" value="RIBOSOMAL_S2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00963">
        <property type="entry name" value="RIBOSOMAL_S2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ83987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="249" mass="27594" checksum="BF015A97925A1BD7" modified="2013-07-24" version="1">MGVVSMKQLLEAGVHFGHQTRRWNPKMAPYIFTERNGIYIIDLQKTVKKLDEAYMFVRDLAAQGESVLFVGTKKQAGDSIKEEAQRAGAYYVNARWLGGMMTNFTTIQRRIKRLEQLHEMETDGTFDLLPKKEVSKLQLEIEKLEKFLGGIKDMKKLPGALFIVDPRKERIAVAEAKKLNIPIVAIVDTNCDPDEIDYVIPGNDDAIRAVKLIAGVMADAIIEGRQGADAEAAAAAAEEAAEETAEAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5VUE8</accession>
    <name>R5VUE8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">50S ribosomal protein L9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rplI</name>
        <name evidence="10" type="ORF">BN566_01867</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the bacterial ribosomal protein bL9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000192">
        <property type="protein sequence ID" value="CCZ84142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01867"/>
    <dbReference type="EnsemblBacteria" id="CCZ84142">
        <property type="protein sequence ID" value="CCZ84142"/>
        <property type="gene ID" value="BN566_01867"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.430.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00503">
        <property type="entry name" value="Ribosomal_L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000244">
        <property type="entry name" value="Ribosomal_L9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009027">
        <property type="entry name" value="Ribosomal_L9/RNase_H1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020594">
        <property type="entry name" value="Ribosomal_L9_bac/chp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020069">
        <property type="entry name" value="Ribosomal_L9_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036791">
        <property type="entry name" value="Ribosomal_L9_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020070">
        <property type="entry name" value="Ribosomal_L9_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036935">
        <property type="entry name" value="Ribosomal_L9_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21368">
        <property type="entry name" value="PTHR21368"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03948">
        <property type="entry name" value="Ribosomal_L9_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01281">
        <property type="entry name" value="Ribosomal_L9_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55653">
        <property type="entry name" value="SSF55653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55658">
        <property type="entry name" value="SSF55658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00158">
        <property type="entry name" value="L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7 10" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 7" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="RIBOSOMAL_L9" evidence="8">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal_L9_C" evidence="9">
        <location>
            <begin position="63"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ84142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="148" mass="15711" checksum="E1A54F2225C56CA7" modified="2013-07-24" version="1">MKVIFLQDVKGSGKKGEVKNVADGYARNMLLKKGLAMEATPKALSELAGKQSSAAHKIDVEKQQAQETADLLNGKTVKATAKAGNGGRLFGAITAGNIADLIAQQYGCKVDKKRISLKTDIKNFGTYEAEIRLYAGILAKVTVEVGEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5VUL0</accession>
    <name>R5VUL0_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ACT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01937</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01937"/>
    <dbReference type="EnsemblBacteria" id="CCZ84212">
        <property type="protein sequence ID" value="CCZ84212"/>
        <property type="gene ID" value="BN566_01937"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045739">
        <property type="entry name" value="ACT_dom_pair"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19571">
        <property type="entry name" value="ACT_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACT" evidence="1">
        <location>
            <begin position="71"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="143" mass="15410" checksum="4CF099018954DDEE" modified="2013-07-24" version="1">MAIQQVSVFVQNKPGALSAICKLLAESGVDMRAMFISDTKEFSILRLIADDTEKAAEVLRDQNCIVSINPVIGVAVPDAPGGIAGMLRVLSETGMNVEYTYAFVAHPGERAYVVLRVTDIAAAEDILKKAGFLLITEQDIQNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5VV17</accession>
    <name>R5VV17_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00269</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000220">
        <property type="protein sequence ID" value="CCZ84362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84362">
        <property type="protein sequence ID" value="CCZ84362"/>
        <property type="gene ID" value="BN566_00269"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="99" mass="11252" checksum="9C927AD1E57A66BC" modified="2013-07-24" version="1">MDATTFPLPLAYHIVLCVLGCVFFLAQFIRLKKPYQLMMAIAMPASLLIYIQPESRTLFYTIGLLVALDLLLALVISLIQRFRHPQEKKSDQQQEAAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VV27</accession>
    <name>R5VV27_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00279</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000221">
        <property type="protein sequence ID" value="CCZ84372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84372">
        <property type="protein sequence ID" value="CCZ84372"/>
        <property type="gene ID" value="BN566_00279"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="334" mass="38781" checksum="7629A5B1CB896685" modified="2013-07-24" version="1">MRLDYCCIAAAAFDDIRINRTLYEIVHLSDFLCFPFKCTNKFFANDFPLPFWVRYTMQLFHENILCIDPYKMDVPVCKCCFDFIAFILPQESMVNKDTSQLVANCLREQSCCNGAINTARQCQQYLSIANLFPNLFDGSLCIVFHRPITRTSTNLIQEIMQHLLTIYGMIYFRMELYAIELLFRIFHCCYRTAGRMCRNRKAFRQLLNIVGMAHPRDCFFRQTGEQQAFRLIFCQCLAVFSGFFRGSNCSAQRICHQLAAVADAQNRNAQFKNSRVYVRRFWVINAVRPTGKDDANWISCSQFCNRCCIPHDFAVNSTFSDSACNQLIILPAKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVL0</accession>
    <name>R5VVL0_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 2">50S ribosomal protein L24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rplX</name>
        <name evidence="3" type="ORF">BN566_00068</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">One of the proteins that surrounds the polypeptide exit tunnel on the outside of the subunit.</text>
    </comment>
    <comment type="function">
        <text evidence="2">One of two assembly initiator proteins, it binds directly to the 5'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uL24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000241">
        <property type="protein sequence ID" value="CCZ84522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00068"/>
    <dbReference type="EnsemblBacteria" id="CCZ84522">
        <property type="protein sequence ID" value="CCZ84522"/>
        <property type="gene ID" value="BN566_00068"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06089">
        <property type="entry name" value="KOW_RPL26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01326_B">
        <property type="entry name" value="Ribosomal_L24_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041988">
        <property type="entry name" value="KOW_RPL26/RPL24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003256">
        <property type="entry name" value="Ribosomal_L24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12903">
        <property type="entry name" value="PTHR12903"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17136">
        <property type="entry name" value="ribosomal_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01079">
        <property type="entry name" value="rplX_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 3" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="117" mass="12688" checksum="6ED89EE746513C2F" modified="2013-07-24" version="1">MSKVHVKTGDTVVLLTGKDIYQYKDVTDKSKGRLEGKVVAVSPKEGKVIVEGFNKITKHVKPTRMGQSGGIVETEGALYASKVQLVCPKCGKPTRIGHAVEDGKKLRVCKKCGKSFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVM4</accession>
    <name>R5VVM4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA repair protein radc</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00408</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000243">
        <property type="protein sequence ID" value="CCZ84547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00408"/>
    <dbReference type="EnsemblBacteria" id="CCZ84547">
        <property type="protein sequence ID" value="CCZ84547"/>
        <property type="gene ID" value="BN566_00408"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="SSF47781"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="95" mass="10860" checksum="BEA2ECBEC5493042" modified="2013-07-24" version="1">MTEKEETDAKQAPKKNLNQGHRSRMRKRYLTCGFSGFQEHEILELLLFYALPRVNTNPIAHQLLKEFHSLAGVLDADPVELQKVPGVREQAMSLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVT2</accession>
    <name>R5VVT2_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">16S rRNA (cytosine(967)-C(5))-methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.176</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">16S rRNA m5C967 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">rRNA (cytosine-C(5)-)-methyltransferase RsmB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BN566_00485</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>cytidine(967) in 16S rRNA + S-adenosyl-L-methionine = 5-methylcytidine(967) in 16S rRNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42748"/>
            <dbReference type="Rhea" id="RHEA-COMP:10219"/>
            <dbReference type="Rhea" id="RHEA-COMP:10220"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74483"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.176"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.176" evidence="4"/>
    <dbReference type="EMBL" id="CBAR010000257">
        <property type="protein sequence ID" value="CCZ84632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00485"/>
    <dbReference type="EnsemblBacteria" id="CCZ84632">
        <property type="protein sequence ID" value="CCZ84632"/>
        <property type="gene ID" value="BN566_00485"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008649">
        <property type="term" value="F:rRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.940.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031341">
        <property type="entry name" value="Methyltr_RsmF_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB/NOP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035926">
        <property type="entry name" value="NusB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006027">
        <property type="entry name" value="NusB_RsmB_TIM44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004573">
        <property type="entry name" value="rRNA_ssu_MeTfrase_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="PTHR22807"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17125">
        <property type="entry name" value="Methyltr_RsmF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01029">
        <property type="entry name" value="NusB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48013">
        <property type="entry name" value="SSF48013"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00563">
        <property type="entry name" value="rsmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11 13" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 11 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM_MT_RSMB_NOP" evidence="12">
        <location>
            <begin position="167"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="11">
        <location>
            <begin position="254"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="11">
        <location>
            <position position="374"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="305"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="322"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CCZ84632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="436" mass="48504" checksum="A46D024C52650D9E" modified="2013-07-24" version="1">MQGRKFAVQLLDQTEQAGSYSNLLLDHALHQVEMEQKEKNLCTVLFYGVLTRRITLDAILKKYSKKPVAKLDPTVRNILHVGLYQLLYLNHIPDRAAVDESVKLTKQCRRASASGFVNAVLRSFLRDEKQIPLPKQKKQAISIQYAAPLWLVDLLLKQYGETETIAFLENALQPAPLTIRRNPLLATEEQLLEALQEHQIQKHPLVPDAYFLKGGNLRNHPAFQKGWFHVQDAASQICCRAVGAKPEETVLDVCSAPGGKTCTIAEYMQGTGQILAFELQPKRVPLIAKAAERLHLSNVTAQQGDASVYNSALPQADRVLCDVPCSGIGVIRRKPEIRYKNPESFADLPAIQAAILETASRYVKVGGTLLYSTCTIRQEENADVVTAFLAKHSEFVPQSVLPEWGGKWAESIVTLLPSDCNSDGFFLAKLQRVQEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVT6</accession>
    <name>R5VVT6_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thiamine diphosphokinase</fullName>
            <ecNumber evidence="1">2.7.6.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_00490</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.2" evidence="1"/>
    <dbReference type="EMBL" id="CBAR010000257">
        <property type="protein sequence ID" value="CCZ84637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00490"/>
    <dbReference type="EnsemblBacteria" id="CCZ84637">
        <property type="protein sequence ID" value="CCZ84637"/>
        <property type="gene ID" value="BN566_00490"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030975">
        <property type="term" value="F:thiamine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004788">
        <property type="term" value="F:thiamine diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006772">
        <property type="term" value="P:thiamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07995">
        <property type="entry name" value="TPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006282">
        <property type="entry name" value="Thi_PPkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007373">
        <property type="entry name" value="Thiamin_PyroPKinase_B1-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036371">
        <property type="entry name" value="TPK_B1-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007371">
        <property type="entry name" value="TPK_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036759">
        <property type="entry name" value="TPK_catalytic_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04265">
        <property type="entry name" value="TPK_B1_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04263">
        <property type="entry name" value="TPK_catalytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00983">
        <property type="entry name" value="TPK_B1_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63862">
        <property type="entry name" value="SSF63862"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63999">
        <property type="entry name" value="SSF63999"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01378">
        <property type="entry name" value="thi_PPkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TPK_B1_binding" evidence="6">
        <location>
            <begin position="134"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ84637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="207" mass="22473" checksum="6864A6EC628EECBD" modified="2013-07-24" version="1">MKRAVIFSGGAFEKPDWVALPKDAMILCADSGLRHARALGISPDWVLGDFDSSSEQPEGESVLRYPPEKDDTDTMLAVKQALSLGAEEIQIYGGLGGRFDHSIANVQTLRYLSEHGAVGSLCDAQNWMTVQTAGTVRQYPRRDGWYFSLLSLSDTCTGVTITGAKYCLQNGILHANFPLGISNEIVAEQAEISLQTGTLLVLYTKDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVW0</accession>
    <name>R5VVW0_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Citrate synthase (unknown stereospecificity)</fullName>
            <ecNumber evidence="2">2.3.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00515</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Carbohydrate metabolism; tricarboxylic acid cycle.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.16" evidence="2"/>
    <dbReference type="EMBL" id="CBAR010000267">
        <property type="protein sequence ID" value="CCZ84662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00515"/>
    <dbReference type="EnsemblBacteria" id="CCZ84662">
        <property type="protein sequence ID" value="CCZ84662"/>
        <property type="gene ID" value="BN566_00515"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00223"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036440">
        <property type="term" value="F:citrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016142">
        <property type="entry name" value="Citrate_synth-like_lrg_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016143">
        <property type="entry name" value="Citrate_synth-like_sm_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002020">
        <property type="entry name" value="Citrate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036969">
        <property type="entry name" value="Citrate_synthase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11739">
        <property type="entry name" value="PTHR11739"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00285">
        <property type="entry name" value="Citrate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00143">
        <property type="entry name" value="CITRTSNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48256">
        <property type="entry name" value="SSF48256"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="513" mass="58359" checksum="560156A0905121B5" modified="2013-07-24" version="1">MPSLTETKDLCYTIDCVHSISEEIETTLFLIHSPKTRFRKDDDYHMLTKEAILKRPELQQIVQKYRHNIERSEQLALQYQENYQINPSFYEQYDVKCGLRDADGRGVLAGLTGISKVQATKLENGVQVPAEGELFYRGYSISDLVNGFIQQNRFGFEEIIYLLLTGELPKADELKSFHELLADYQNLPAHFKSSVIMRMPSDNIMNGMEKCVLALHSTDPKPDDISIPNVMRQCLELIARFPSLAVYNYQMTQYFHKGSSMVLRKPKPDKTIAENLLTMMRPDGKYTQLEARLLDLALVLHAEHGGGNNSTFTMHVVTSSGTDTYSAISASLGSLKGPKHGGANLKVMEMFQDLKAHVTDWNNRDAIRSYLTGLLNREGFDHSGLIYGLGHAVYTVSDPRARILKKFAEMLSNEKQMHDEFQLYQTVEEIATELLLERRKTNKGVCANVDFYSGFVYEMLGIPMELYTPIFAIARIAGWSAHRIEELTNSNKIIRPAYRPVGLERTYVPLGER</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5UWV3</accession>
    <name>R5UWV3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Bacterial nucleoid DNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00608</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial histone-like protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000008">
        <property type="protein sequence ID" value="CCZ82803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00608"/>
    <dbReference type="EnsemblBacteria" id="CCZ82803">
        <property type="protein sequence ID" value="CCZ82803"/>
        <property type="gene ID" value="BN566_00608"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030261">
        <property type="term" value="P:chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000119">
        <property type="entry name" value="Hist_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010992">
        <property type="entry name" value="IHF-like_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33175">
        <property type="entry name" value="PTHR33175"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00216">
        <property type="entry name" value="Bac_DNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01727">
        <property type="entry name" value="DNABINDINGHU"/>
    </dbReference>
    <dbReference type="SMART" id="SM00411">
        <property type="entry name" value="BHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47729">
        <property type="entry name" value="SSF47729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ82803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="92" mass="10333" checksum="1A5CBE02D14FFBEE" modified="2013-07-24" version="1">MTKTELIQGVQERLDCTKKDAELAVNTVFEVLTDALVHEEKVAISKFGSFEVRQRNEKLCKNPQTQELMIVPASKAPVFKASKTLKDAVNQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5UX02</accession>
    <name>R5UX02_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00648</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000012">
        <property type="protein sequence ID" value="CCZ82843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00648"/>
    <dbReference type="EnsemblBacteria" id="CCZ82843">
        <property type="protein sequence ID" value="CCZ82843"/>
        <property type="gene ID" value="BN566_00648"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004518">
        <property type="entry name" value="MazG_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011551">
        <property type="entry name" value="NTP_PyrPHydrolase_MazG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30522">
        <property type="entry name" value="PTHR30522"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03819">
        <property type="entry name" value="MazG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00444">
        <property type="entry name" value="mazG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MazG" evidence="1">
        <location>
            <begin position="34"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="MazG" evidence="1">
        <location>
            <begin position="172"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="270" mass="30412" checksum="74CED18874BA992E" modified="2013-07-24" version="1">MMTYPQKERYDINDLIEVIRMLRLPDGCPWDKVQTHESIRMNFIEEVYEVIEAIDKNDTALMREELGDVLMQVVFHAQMEREQGNFDFDDVCDEVCRKLIIRHPHVFSNVQAGNTDEAVNSWEQVKQQTKGQTTYTETLESVAVSLPALMRAQKVGKRAAKSGFDFANTADTFAKLQSEVTELQEAIQAKDSDAAAEELGDLLFSCTNLARALDVDAEETLTAATEKFIRRFAKVEQAATAAGKKMKDCSPEELETFWQQAKGAKTDAIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>R5UX60</accession>
    <name>R5UX60_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA (guanine-N(7)-)-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA (guanine(46)-N(7))-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA(m7G46)-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trmB</name>
        <name evidence="2" type="ORF">BN566_00695</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>guanosine(46) in tRNA + S-adenosyl-L-methionine = N(7)-methylguanosine(46) in tRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42708"/>
            <dbReference type="Rhea" id="RHEA-COMP:10188"/>
            <dbReference type="Rhea" id="RHEA-COMP:10189"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74480"/>
            <dbReference type="EC" id="2.1.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">tRNA modification; N(7)-methylguanine-tRNA biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.33" evidence="1"/>
    <dbReference type="EMBL" id="CBAR010000022">
        <property type="protein sequence ID" value="CCZ82893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00695"/>
    <dbReference type="EnsemblBacteria" id="CCZ82893">
        <property type="protein sequence ID" value="CCZ82893"/>
        <property type="gene ID" value="BN566_00695"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00989"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008176">
        <property type="term" value="F:tRNA (guanine-N7-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01057">
        <property type="entry name" value="tRNA_methyltr_TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003358">
        <property type="entry name" value="tRNA_(Gua-N-7)_MeTrfase_Trmb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23417">
        <property type="entry name" value="PTHR23417"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02390">
        <property type="entry name" value="Methyltransf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00091">
        <property type="entry name" value="TIGR00091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51625">
        <property type="entry name" value="SAM_MT_TRMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="1 2" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0819">tRNA processing</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="222" mass="26302" checksum="0CCDC81C3DB4C57F" modified="2013-07-24" version="1">MRIRHKPWAKPELEACPFYISEPKLNRGHWNTIFPGEKKPLRLELGCGKGGFISQEAPANPDYHYIAMDIKNEMLVLAKRKLEAAYAEQQLPLDNVRIFICNLMQFSEYFSPEDRIDRIYINFCNPWPKGKHKKRRLTHPRQLQQYRTVLDGEIWFKTDDDELFEESILYFQECGFDIVFQTTDLHKEPDIQSFRTEHEAMFEEMGKTIKFLIARPQQASAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXE6</accession>
    <name>R5UXE6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pyridine nucleotide-disulfide oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00773</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000034">
        <property type="protein sequence ID" value="CCZ82973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00773"/>
    <dbReference type="EnsemblBacteria" id="CCZ82973">
        <property type="protein sequence ID" value="CCZ82973"/>
        <property type="gene ID" value="BN566_00773"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="422"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="526" mass="56249" checksum="93B38BD45AC9E1F9" modified="2013-07-24" version="1">MKRYDFLIVGAGPAGLSAAIEAAKRGMQVAVFDENAKPGGQLFKQIHKFFGSKEHKAKIRGFVIGQQLLEEAQKLGVSVILNATVIGLYAEKELVVRIGEEIHHYKGDAVLVATGAAENMVTFDGWTLPGVIGAGAAQTMMNLHGIRPGKKILMLGSGNVGLVVSYQLLQAGCKVVALVDAAPRVGGYGVHAAKIARCGVPFYLSHTIQKAEGTDHVTGVTIAEVDSHFQFIPGTEKHFEVDTICLAVGLSPMSQLLKMAGCKMEDNPKRGGQVPICDAYGETSVPGIFAAGDVSGIEEASSAMIEGRISGIAIAASLGFLEESEKQEQIAANEAALETLRQGMFAPCNRGKLVEKTEEGIDTAMHLLKSGYLTEEEVEKFPGVTRNKKIHPVIECVQNIPCNPCQDACPKHCIKIGSHITALPAVDQEVECIGCGLCVSSCSGQAIFLVQEECDEPGYGTVTLPYEFLPLPKKGDRGFGYDRGGKKVCEAEVVSVKTAKAFDHTNLLTIKVPTDMVMRARFYQAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXI6</accession>
    <name>R5UXI6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BFD-like [2Fe-2S] binding domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00590</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000008">
        <property type="protein sequence ID" value="CCZ82785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00590"/>
    <dbReference type="EnsemblBacteria" id="CCZ82785">
        <property type="protein sequence ID" value="CCZ82785"/>
        <property type="gene ID" value="BN566_00590"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007419">
        <property type="entry name" value="BFD-like_2Fe2S-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041854">
        <property type="entry name" value="BFD-like_2Fe2S-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04324">
        <property type="entry name" value="Fer2_BFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fer2_BFD" evidence="1">
        <location>
            <begin position="22"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="110" mass="12458" checksum="093AABB40639585F" modified="2013-07-24" version="1">MRKMIRVANPEPFVAKDDDDMIICRCEEITKGEIRKAVYDGMRTTNEVKRYLRAGMGLCQGQTCQRLVQGIIAAELKTRPSELGMIQGRAPVRPVEILTFSKNITDVKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXL9</accession>
    <name>R5UXL9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPF0020 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00625</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000011">
        <property type="protein sequence ID" value="CCZ82820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00625"/>
    <dbReference type="EnsemblBacteria" id="CCZ82820">
        <property type="protein sequence ID" value="CCZ82820"/>
        <property type="gene ID" value="BN566_00625"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034470">
        <property type="term" value="P:ncRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001510">
        <property type="term" value="P:RNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000241">
        <property type="entry name" value="RNA_methylase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01170">
        <property type="entry name" value="UPF0020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00981">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01261">
        <property type="entry name" value="UPF0020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="UPF0020" evidence="2">
        <location>
            <begin position="197"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ82820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="373" mass="41954" checksum="0D6D6929B64FB676" modified="2013-07-24" version="1">MQHTFWMCCPCHFGLEHTLHFELKKLGAEQIQVQDGRILFRGDYHLLAMANLCLSTAERVLIQLGSFPAYNFEELFQGMKKLPLEEWIGKDDAFPVKGHALDSKLHSVPDCQSILKKAAVERLGQKYQTSWFSESGAVHQIQFSIRKNLVTIYLDSSGVGLHKRGYRRNANAAPIKETLAAGIVDLARVKQNSVVCDPFCGSGTLLIEAAFKAMHIAPGLHRHFAAERWDCIPASVWANARAEAIAAIRRDTTFEAYGFDCDPEAIRLSEENIRKAGVKTRIHVMQQDIADFTAVPEQIIICNPPYGERLLDVQAAESLYQIMGSVFPANKQYPCYVISPDANFESFFGKPATKRRKLYNGMIPCQLFSYYGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXS1</accession>
    <name>R5UXS1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00900</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000059">
        <property type="protein sequence ID" value="CCZ83113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00900"/>
    <dbReference type="EnsemblBacteria" id="CCZ83113">
        <property type="protein sequence ID" value="CCZ83113"/>
        <property type="gene ID" value="BN566_00900"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_25" evidence="1">
        <location>
            <begin position="48"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="265" mass="29834" checksum="CA33EAE8FC23E39C" modified="2013-07-24" version="1">MQNQNIDHGNKFDWGKTSADYARFRDIYPPQFYEMILNRNCGAAGQRVLDVGTGTGVLPRNLYAAGSHWTGCDIAPNQIAQAKQLSQGMEIAYLVSTAETLPTFAEPFDWITACQCYWYFDVAKTAPAFARVLKSGGKVLLLCMQWLPYEDPIAHASEELVLQYNPNWSGAGERKHPIAIAEEWLDDFTVTHQMECDLDVPFTRESWNGRMKACRGIGASLPPEEIAAWEAEHLQLLERIAPESFSVRHYAALAELTKKSEKEVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXX0</accession>
    <name>R5UXX0_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Radical SAM core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_00951</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the radical SAM superfamily. Anaerobic sulfatase-maturating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000069">
        <property type="protein sequence ID" value="CCZ83168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00951"/>
    <dbReference type="EnsemblBacteria" id="CCZ83168">
        <property type="protein sequence ID" value="CCZ83168"/>
        <property type="gene ID" value="BN566_00951"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023885">
        <property type="entry name" value="4Fe4S-binding_SPASM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000385">
        <property type="entry name" value="MoaA_NifB_PqqE_Fe-S-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023867">
        <property type="entry name" value="Sulphatase_maturase_rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43273">
        <property type="entry name" value="PTHR43273"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01384">
        <property type="entry name" value="thioether_bond_formation_requi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04085">
        <property type="entry name" value="rSAM_more_4Fe4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01305">
        <property type="entry name" value="MOAA_NIFB_PQQE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="8"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="353" mass="39957" checksum="1B22AD5CC7AAF73A" modified="2013-07-24" version="1">MHVTLHLTDQCNLDCSYCVHEKRNCVMSETVLNAACDYIFQSGTTAGICFFGGEPLLEKGLLQKAVTRCQQHAKETGIPFQCKMTTNGTLLDKEFIQYAKSIGMHIGLSFDGTGQDRCRRYRDGKGTFSDLERNAKLLLEQLPNSYAMMTIAPEAVDTYTDSVKYLYRLGFRNMNATLAYGKRVHWTDAHLEQLQAELQKTAAFYEQQFLQGTPFYFGTFDGKIQDSIRGHCSGERCHLGLRQMPVTPDGSLYPCTQFINDPAYLLGDVFHGLDKEKVRKLIHREATPNRCQECALKNRCTNACGCMNRLETGDENQVSPLQCTYERMLIATCDAVANRMMEQKPEAFLKKFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXX4</accession>
    <name>R5UXX4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00956</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000069">
        <property type="protein sequence ID" value="CCZ83173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00956"/>
    <dbReference type="EnsemblBacteria" id="CCZ83173">
        <property type="protein sequence ID" value="CCZ83173"/>
        <property type="gene ID" value="BN566_00956"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070567">
        <property type="term" value="F:cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02516">
        <property type="entry name" value="CDP-ME_synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034683">
        <property type="entry name" value="IspD/TarI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01128">
        <property type="entry name" value="IspD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="237" mass="26039" checksum="D0126B673906C73C" modified="2013-07-24" version="1">MTSSPDTSGILVCAGNATRMQGQNKILLPLGQTNVIGMSMLAMEHCDRIAEFIVVARPSDHDAIQKTIADLQLKKCSAIVPGGATRQESVLNGVRAASKETKLFAIHDGARPLVKPEHIAAVIHDAKVFGGATLGVPVKDTIKVVNDGLIVDTPYRPHLYSTQTPQVFQKRIYFEAVDFALEHHLDFTDDCQLAEAINCKVYMTIGDYTNLKITTPEDIFIARMLLEKRMEESTCTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5UY28</accession>
    <name>R5UY28_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein conserved in bacteria</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01014</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000080">
        <property type="protein sequence ID" value="CCZ83233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01014"/>
    <dbReference type="EnsemblBacteria" id="CCZ83233">
        <property type="protein sequence ID" value="CCZ83233"/>
        <property type="gene ID" value="BN566_01014"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05826">
        <property type="entry name" value="Sortase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005754">
        <property type="entry name" value="Sortase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023365">
        <property type="entry name" value="Sortase_dom-sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009835">
        <property type="entry name" value="SrtB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04203">
        <property type="entry name" value="Sortase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63817">
        <property type="entry name" value="SSF63817"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="427" mass="48815" checksum="D1CCFB6AE486CB53" modified="2013-07-24" version="1">MSEHLKSPASTSESEQTKQQAKMNAIRSSLQKLEEEEEEEMLQQRIAANIQLAKEKKAFPERFVPKPPPPVKHPVRKRKKRTKRQILRDLFPKRGDGFGETMRKCIFWISILIFGACLVLMIQYLFGLWQSKKLYDEIGSNYHNVATTQVVTEAQTEESALEETEVPTETTTEVKHYSVMEGAANLLSINPEVIGYLSIPDTEVDYPVMQHPNDVEGDEYYLHHDLQGNRSQNGSIFLDSRCSFDAVGADGTLAVPNSDNLIVYGHDMRDRSMFGSLRDYRSVDGYYEAHPLIFLNSNYETYVFKIYAYFIADAEDTTDTRFDYWNRINFDDETAFYDYVNEVKRRTLRNTSVDVKYGDQLLTLSTCNGAFSTARLVICARMVRAGEDPHAGTTGSTPNTNIKWPSVYYYGNENTYDPNAPFVPYGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5UY34</accession>
    <name>R5UY34_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00770</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000033">
        <property type="protein sequence ID" value="CCZ82970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82970">
        <property type="protein sequence ID" value="CCZ82970"/>
        <property type="gene ID" value="BN566_00770"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004394770" evidence="2">
        <location>
            <begin position="30"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="392"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ82970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="421" mass="46317" checksum="0205DB0B3648B088" modified="2013-07-24" version="1">MRRRKTWIAVTCMLSLTLAGSLFGNHAFAADGNGIGEHIGHAAAETTTSIEEELTEGFTNAGNVATLPVPNAEENSNTGREKFIFADKKSAENEKIVCYLSTNPLYENLEKIQNGGVAFCINGGETIDGIPVYTWLNQVDSQESYFFLEDSFEIKDGDNIYISLGDEDETFLKEFFKEDEIFTFNDIENKDVVTSDEVSLTLSEDGKLKIDTSAAPGSNVLTLTMEEFNSLKNDSSNYGIGKCKDGTISLVKKVYSAQKTLHNQYIIEKSGKEFQFCGGKDIYALQKDVFGSDENLNFEDIIQAAKESGEDDDFGLVLDTAEQPLLVYRGGELKASTETTTQTSITVEQSSDTVTETVLEANQEEASSAITSQTASEEEQETVSNESKKYKWISIGLLCAFVLIGAGWILVPPMLKKNKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5UY42</accession>
    <name>R5UY42_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01029</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000081">
        <property type="protein sequence ID" value="CCZ83248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01029"/>
    <dbReference type="EnsemblBacteria" id="CCZ83248">
        <property type="protein sequence ID" value="CCZ83248"/>
        <property type="gene ID" value="BN566_01029"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045678">
        <property type="entry name" value="DUF6198"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19700">
        <property type="entry name" value="DUF6198"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="185"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="227" mass="25157" checksum="3D56B4D80BCECC69" modified="2013-07-24" version="1">MLQEKSMPKIYGELALLLAVCINSLGVVLMLYSGAGISAISSVPFAFSEVLPHLSLGTWTYLFQGLLILSLMILRKQFVPQYLCSFLVGFVFSELLDVHEAWIEILPTTLPWKFLYFAMSYLLICIGIALSNRCGLPIIPTDLFPRELSQITAVGYPKIKIGFDVTCLAVTAALTLLFLHRLDGLGIGTILAAFTMGKGVGIIGNWMDRRFQFVSVLANKLTWFKPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYC5</accession>
    <name>R5UYC5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SpoVT/AbrB family regulatory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00813</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000039">
        <property type="protein sequence ID" value="CCZ83020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00813"/>
    <dbReference type="EnsemblBacteria" id="CCZ83020">
        <property type="protein sequence ID" value="CCZ83020"/>
        <property type="gene ID" value="BN566_00813"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007159">
        <property type="entry name" value="SpoVT-AbrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037914">
        <property type="entry name" value="SpoVT-AbrB_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04014">
        <property type="entry name" value="MazE_antitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00966">
        <property type="entry name" value="SpoVT_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89447">
        <property type="entry name" value="SSF89447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01439">
        <property type="entry name" value="lp_hng_hel_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SpoVT-AbrB" evidence="1">
        <location>
            <begin position="8"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="81" mass="9431" checksum="D725C495BEA71BB1" modified="2013-07-24" version="1">MKDSGIMRRVDSLGRFVLPKELRRQLQIDCNDYLQIYTEGTKIIIQKAQSSCALCNKTEDLIDFNDKKVCRSCVKLIKDYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYJ5</accession>
    <name>R5UYJ5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative AAA-ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00115</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000057">
        <property type="protein sequence ID" value="CCZ83100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00115"/>
    <dbReference type="EnsemblBacteria" id="CCZ83100">
        <property type="protein sequence ID" value="CCZ83100"/>
        <property type="gene ID" value="BN566_00115"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="116" mass="13627" checksum="BE47DB67E4B6774D" modified="2013-07-24" version="1">MNRYFNITGCCNPQEHYMVNLDSRLAQIKKMVDKGQYFSINKGRQYGKTTILKALREHLKNDYVVISMDFQFVSTSEFATENRFVKAFARLLWSRCFCVSVFNPHPYVRGDCQACF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYN4</accession>
    <name>R5UYN4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">YkuD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_00932</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000064">
        <property type="protein sequence ID" value="CCZ83145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00932"/>
    <dbReference type="EnsemblBacteria" id="CCZ83145">
        <property type="protein sequence ID" value="CCZ83145"/>
        <property type="gene ID" value="BN566_00932"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16913">
        <property type="entry name" value="YkuD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038054">
        <property type="entry name" value="LD_TPept-like_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005490">
        <property type="entry name" value="LD_TPept_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038063">
        <property type="entry name" value="Transpep_catalytic_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03734">
        <property type="entry name" value="YkuD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141523">
        <property type="entry name" value="SSF141523"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143985">
        <property type="entry name" value="SSF143985"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="99"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="YkuD" evidence="5">
        <location>
            <begin position="432"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="28"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ83145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="556" mass="61954" checksum="05C240D0A8751EAD" modified="2013-07-24" version="1">MQAALQNGQGTTRKRPPRPYTEAEAESGSEQTKTRKPPRRRTFPAEHLNHDTVDMDADMGSDGYDMNETENRPQPVKRMPRPKPEPAEEAVKSGNGKKIALLVSLLVIVALLAVYLVGFFSYQNCMLPNTRVNGIDVSGMTAAEAEQNILSQANAKGITFVKKDGEEVPFSGDQFGSTVSLSSNTVLTNAAQSGHGAWFTSYFHPTDLTVSVVNSYSEEDLADLIKNYTWGTVAPTDAKIEKGDDDTYQIVPEEEGDMVDTDTLAAYTLEQMKDGNNTISLTDANCYLQPEITSDDLQDKLAEYNRFSDLTITYDFDDRQEVLEPDTISEWITVNEEDDSVEVDRDAVTNWVNENLVKYDTYGSDYMRTFHSTLQGTLEIPGGQYGIYGWLTDVEATVDKLVEYIQNGESVTVEPEYARTGYCRATDDIGDSYAEVDITNQKVFVYKDGELVIESDCVTGMANDPSRETPPGVYKVWSMDRNRILGTMEAYGYEQPVDYWIYFTEIDIGFHDLLRSAYGGEIYKTNGSHGCVNLPLDVAAEMYDTVEIGYPVIVIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYP3</accession>
    <name>R5UYP3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidyl-tRNA hydrolase</fullName>
            <shortName evidence="1">PTH</shortName>
            <ecNumber evidence="1">3.1.1.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pth</name>
        <name evidence="2" type="ORF">BN566_00116</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>an N-acyl-L-alpha-aminoacyl-tRNA + H2O = a tRNA + an N-acyl-L-amino acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:13883"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59874"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:138191"/>
            <dbReference type="EC" id="3.1.1.29"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PTH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.29" evidence="1"/>
    <dbReference type="EMBL" id="CBAR010000068">
        <property type="protein sequence ID" value="CCZ83155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00116"/>
    <dbReference type="EnsemblBacteria" id="CCZ83155">
        <property type="protein sequence ID" value="CCZ83155"/>
        <property type="gene ID" value="BN566_00116"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004045">
        <property type="term" value="F:aminoacyl-tRNA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00462">
        <property type="entry name" value="PTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00083">
        <property type="entry name" value="Pept_tRNA_hydro_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001328">
        <property type="entry name" value="Pept_tRNA_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018171">
        <property type="entry name" value="Pept_tRNA_hydro_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036416">
        <property type="entry name" value="Pept_tRNA_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17224">
        <property type="entry name" value="PTHR17224"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01195">
        <property type="entry name" value="Pept_tRNA_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53178">
        <property type="entry name" value="SSF53178"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00447">
        <property type="entry name" value="pth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01196">
        <property type="entry name" value="PEPT_TRNA_HYDROL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="207" mass="22837" checksum="5B18583971D3AC7C" modified="2013-07-24" version="1">MGIFDLFKQIEGESHKMGKPTWLIVGLGNPGLEYAETRHNAGFLAITQLAKQHEIALSTMKFRSDCGDGMLGETRCFFMKPATYMNNSGEAVAAAAEFYKIPPERVLVLYDDISLPPGKIRIRRKGSSGGHNGIKSIIALLGSEEFPRVKIGVGEKPRKDYNLADWVLGKFPESDQEAMQQAFEQAGKAAELIVNGKIEEAMSQYSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYU7</accession>
    <name>R5UYU7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PfkB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00998</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000073">
        <property type="protein sequence ID" value="CCZ83215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00998"/>
    <dbReference type="EnsemblBacteria" id="CCZ83215">
        <property type="protein sequence ID" value="CCZ83215"/>
        <property type="gene ID" value="BN566_00998"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PfkB" evidence="1">
        <location>
            <begin position="8"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="325" mass="35532" checksum="E3FB1061CE64E93F" modified="2013-07-24" version="1">MKNYDVTALGELLIDFTESGISSQGNPLLEANPGGAPCNVLAMLSKLGKRTAFIGKVGADMFGRQLAETINEVGICTDGLVTDASVPTTLAFVHTFADGEREFSFIRNPGADMMLSKQEVRTDLIRDSKIFHFGTLSSTHAGVREATRFAIDTAIESGAMLSFDPNLREPLWKNLEDAKTEIEYGFSKCNLLKISDNEVEFLFGHSDYDRAARELLEKYSQMQLICITLGGNGSCAYTRNAIAKAPVYDVKPVEKTGAGDTFFGCVLNYVLEHEADKLTKEQLLELLYFANAGASLITTRKGALKVMPNRQEIQDLMNAGIVSKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZ78</accession>
    <name>R5UZ78_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Stage 0 sporulation protein A homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN566_00160</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000146">
        <property type="protein sequence ID" value="CCZ83633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00160"/>
    <dbReference type="EnsemblBacteria" id="CCZ83633">
        <property type="protein sequence ID" value="CCZ83633"/>
        <property type="gene ID" value="BN566_00160"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Response regulatory" evidence="7">
        <location>
            <begin position="3"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="8">
        <location>
            <begin position="128"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="6">
        <location>
            <begin position="128"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="5">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ83633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="226" mass="26134" checksum="17CEFAB29E15542D" modified="2013-07-24" version="1">MYRLLVVDDEANIRNVVREYAEFEEYEVTEAENGMEAVALCREQDFDLIIMDVMMPRLDGFSACKEIKKRKNIPVIMLSARGEEYDKLFGFELGIDDYVVKPFSPKELMARVKAVLNRHHATAEQVSDQRLKFEGLEIDLAGREVYVDGQRATMTPKEYDLLFYLVKNKGLALSRDKLLEAVWGYDFFGDDRTVDTHIKMLRGSLGVYRKFIVTLRGMGYKFDPDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZ92</accession>
    <name>R5UZ92_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01389</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000147">
        <property type="protein sequence ID" value="CCZ83653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83653">
        <property type="protein sequence ID" value="CCZ83653"/>
        <property type="gene ID" value="BN566_01389"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="77" mass="8394" checksum="4414F180DD8AD725" modified="2013-07-24" version="1">MGKIVIKTTRKSNVSKVKVRGFKEFDAAKIAFCTFIGIMSGLNQEDKNLILCAAATIIQDVADKAGSEPEEEKSCKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZC6</accession>
    <name>R5UZC6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01429</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000153">
        <property type="protein sequence ID" value="CCZ83693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83693">
        <property type="protein sequence ID" value="CCZ83693"/>
        <property type="gene ID" value="BN566_01429"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="303" mass="33623" checksum="575807EA1293672D" modified="2013-07-24" version="1">MPTTSQSAKPPYNNQKRATPANACRNCPFLLEFVWILLCRFCLHGNWLALCIQNFRLLVLGVLVAATFFACSKDDILSMHKTGSPCEHLQELPVFVSICLHFTLRILPARQSACPLHLEFPAACPWCLGCSDFFACSKDNTLSIQKTGSPCEFLQELPVSVLICLGFTLQILPARQSACPLHLKFPAACPLSHIPWSQRPFFTCSKDGTPCINTFHTENRQPLRMLAGTARFCFNLSEFYFADSACTAIGLPSASRISGCLPSVTHSLVTTTFFACSKDGTSYMVCTKMLSMMERRPLAPVFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZC7</accession>
    <name>R5UZC7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Beta-glucosidase-related glycosidases</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_01161</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000094">
        <property type="protein sequence ID" value="CCZ83385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01161"/>
    <dbReference type="EnsemblBacteria" id="CCZ83385">
        <property type="protein sequence ID" value="CCZ83385"/>
        <property type="gene ID" value="BN566_01161"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009044">
        <property type="term" value="F:xylan 1,4-beta-xylosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045493">
        <property type="term" value="P:xylan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044993">
        <property type="entry name" value="BXL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026891">
        <property type="entry name" value="Fn3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002772">
        <property type="entry name" value="Glyco_hydro_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036881">
        <property type="entry name" value="Glyco_hydro_3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001764">
        <property type="entry name" value="Glyco_hydro_3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036962">
        <property type="entry name" value="Glyco_hydro_3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42721">
        <property type="entry name" value="PTHR42721"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14310">
        <property type="entry name" value="Fn3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00933">
        <property type="entry name" value="Glyco_hydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01915">
        <property type="entry name" value="Glyco_hydro_3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00133">
        <property type="entry name" value="GLHYDRLASE3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01217">
        <property type="entry name" value="Fn3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52279">
        <property type="entry name" value="SSF52279"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Fn3_like" evidence="4">
        <location>
            <begin position="599"/>
            <end position="667"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="692" mass="75747" checksum="8C39B77CE6F8CF17" modified="2013-07-24" version="1">MYRTYRKEAEALVQQMTVAEQASQLRYDAPAIPRLGIPAYNWWNEGLHGLARAGTATMFPQAIGMAATFSPELVQEMGEVTATEARAMYHACSEQGDRDIYKGLTLWTPNVNLFRDPRWGRGQETYGEDPYLIATLGTAMVHGMQQDGDYLKVAACAKHFAVHSGPESVRHTFDAEVSAKDLEETYLPAFQALVEDAEVESVMGAYNRVNGDPACASPMLMQKLKDWQFDGYFVSDCWAIRDFHTTHKITETATDSIALALKAGCHLNCGCTYVQILLALEEGKITEDDIFQACVRVMQTRFRLGMFAEQEPYADLPITVLACPEHKAKSLAAAERSMVLLRNEQQILPLQAEQLQTIAVIGANADSRAAMVGNYNGTPDRAITFLEGIQRAFSGRVLYAEGCHLFKDRCSELAQPGDRYAEAVAVAKAADVVILCLGLDATLEGEEGDTGNEFASGDKADLRLPEPQRVLLQKIKATGTPIVLVTATGSAICPELETEAWIQAWYPGSEGGTALANLLFGKCSPSGKLPVTFYADAEKLPAFTDYSMQHRTYRYTQENILYPFGYGLTYGTVVCTDLHYENGIAVVTAENQGSRDTEEVIQLYIKSNADCAVPYHSLCGFQRVFLPAGAKKTISIPLAKNAFTSVDEQGTRGVYGTTFTLYAGISQPDEKSQQLTGTAPAMQVISASELHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZN1</accession>
    <name>R5UZN1_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">3-dehydroquinate synthase</fullName>
            <shortName evidence="8">DHQS</shortName>
            <ecNumber evidence="8">4.2.3.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">aroB</name>
        <name evidence="10" type="ORF">BN566_01548</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the conversion of 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) to dehydroquinate (DHQ).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate = 3-dehydroquinate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21968"/>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
            <dbReference type="EC" id="4.2.3.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 1 divalent metal cation per subunit. Can use either Co(2+) or Zn(2+).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="8">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 2/7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the sugar phosphate cyclases superfamily. Dehydroquinate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.4" evidence="8"/>
    <dbReference type="EMBL" id="CBAR010000165">
        <property type="protein sequence ID" value="CCZ83813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01548"/>
    <dbReference type="EnsemblBacteria" id="CCZ83813">
        <property type="protein sequence ID" value="CCZ83813"/>
        <property type="gene ID" value="BN566_01548"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00085"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003856">
        <property type="term" value="F:3-dehydroquinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00110">
        <property type="entry name" value="DHQ_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016037">
        <property type="entry name" value="DHQ_synth_AroB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030963">
        <property type="entry name" value="DHQ_synth_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030960">
        <property type="entry name" value="DHQS/DOIS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01761">
        <property type="entry name" value="DHQ_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001455">
        <property type="entry name" value="DHQ_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01357">
        <property type="entry name" value="aroB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-0170">Cobalt</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 8" id="KW-0520">NAD</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="DHQ_synthase" evidence="9">
        <location>
            <begin position="67"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="105"/>
            <end position="109"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="129"/>
            <end position="130"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="169"/>
            <end position="172"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ83813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="349" mass="37669" checksum="48D44A58F872F1BD" modified="2013-07-24" version="1">MKTITVHASHTYPIYVQHGLLAECGTYIRTATKAAALAVITDDTVESLYGAAVVSALRNAGFRVEIFVFPHGEASKCAETLLQVYDFLCEKQFTRTDAIVALGGGVVGDLAGFAAATYLRGMDFIQIPTTLLAQVDSSVGGKTAIDLKGGKNLVGAFKQPVCVLCDPDTLRTLPSATFSDGMGEVVKYGMIRDAKLFELLAAHNQETIFSVLEDVICTCISIKRDVVEADEFDTGERMILNYGHTLGHAIEGYYHYETYSHGSAVAIGMCLIAEKTCTPEQTAALKACLQAYHLPTSVPVPISDLLPFCGVDKKRAGNQLRYIVCETPGKAEIRTASISEFAAWMEEQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZV8</accession>
    <name>R5UZV8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Helix-turn-helix domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01274</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000119">
        <property type="protein sequence ID" value="CCZ83505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01274"/>
    <dbReference type="EnsemblBacteria" id="CCZ83505">
        <property type="protein sequence ID" value="CCZ83505"/>
        <property type="gene ID" value="BN566_01274"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="13"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="117" mass="13380" checksum="E625AD97043FB12B" modified="2013-07-24" version="1">MKTIQYKEMGARIRAQRERLGYTREQLAEKLDVSTKFCSDIELGIKGISIQTLAKLSDLLCLRTDYILFGEEQPDHTAELEMMDALIQKCPADRQDDLLTIIHAFIHAVSEPQEPTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>R5V004</accession>
    <name>R5V004_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Anthranilate phosphoribosyltransferase</fullName>
            <ecNumber evidence="3">2.4.2.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">trpD</name>
        <name evidence="7" type="ORF">BN566_01657</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the transfer of the phosphoribosyl group of 5-phosphorylribose-1-pyrophosphate (PRPP) to anthranilate to yield N-(5'-phosphoribosyl)-anthranilate (PRA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>diphosphate + N-(5-phospho-beta-D-ribosyl)anthranilate = 5-phospho-alpha-D-ribose 1-diphosphate + anthranilate</text>
            <dbReference type="Rhea" id="RHEA:11768"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:18277"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="3">Binds 2 magnesium ions per monomer.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the anthranilate phosphoribosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.18" evidence="3"/>
    <dbReference type="EMBL" id="CBAR010000173">
        <property type="protein sequence ID" value="CCZ83928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01657"/>
    <dbReference type="EnsemblBacteria" id="CCZ83928">
        <property type="protein sequence ID" value="CCZ83928"/>
        <property type="gene ID" value="BN566_01657"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00041"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004048">
        <property type="term" value="F:anthranilate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01743">
        <property type="entry name" value="GATase1_Anthranilate_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1030.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00211">
        <property type="entry name" value="TrpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005940">
        <property type="entry name" value="Anthranilate_Pribosyl_Tfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000312">
        <property type="entry name" value="Glycosyl_Trfase_fam3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017459">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036320">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035902">
        <property type="entry name" value="Nuc_phospho_transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006221">
        <property type="entry name" value="TrpG/PapA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02885">
        <property type="entry name" value="Glycos_trans_3N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00591">
        <property type="entry name" value="Glycos_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47648">
        <property type="entry name" value="SSF47648"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52418">
        <property type="entry name" value="SSF52418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01245">
        <property type="entry name" value="trpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00566">
        <property type="entry name" value="trpG_papA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="1 3 7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="GATase" evidence="4">
        <location>
            <begin position="4"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_trans_3N" evidence="6">
        <location>
            <begin position="210"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_3" evidence="5">
        <location>
            <begin position="278"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphoribosylpyrophosphate binding" evidence="3">
        <location>
            <begin position="288"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphoribosylpyrophosphate binding" evidence="3">
        <location>
            <begin position="295"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphoribosylpyrophosphate binding" evidence="3">
        <location>
            <begin position="313"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="285"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>anthranilate</name>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="325"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="371"/>
        </location>
        <ligand>
            <name>anthranilate</name>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="430"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ83928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="544" mass="58277" checksum="46473537470C8CFC" modified="2013-07-24" version="1">MILFLDNYDSFTYNLYQYIGELYPDIQVVRNDEITLEEIEAMHPDALLISPGPGYPKDAGISIPAIQKFAGKMPILGICLGHQAIAEAFGGKIVKAEHLMHGKASNIAIDNQSVLFAGLPQVIACGRYHSLIVEEASLPSCLRITARDENGQIMALEHTKFPIYGLQFHPESILSEDGKEILIRFLNLLPGVSIPTKQQTPQQPKKALLPYAEKVLNGGNLTETEAMEAMDLIMSNAATDIQIAEFLTALRMKGETIEEITGFAKGMRAKAKTVPNCKDAIDIVGTGGDCSNSFNISTTSAFIIAAAGAKVAKHGNRSVSSKSGAADVLERLGVKIQTTPEQAANCIHEIGISFLFAQSYHGSMKYVAKARKEMGTRTVFNILGPLANPAETDYILLGVYSDDLIPVMAKVLCGLGIRHAMVVHGEDGFDEISISAPTKVAEIKDGGIFEYTITPEQFGLPLAEKEEVVGGSPEENALITQGILDGTIRDAKRNIVLLNAGCALYVVGQAGSIGDGVALAAEMIDSGAALAKLEELKQFTNALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0A1</accession>
    <name>R5V0A1_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 10">ATP-dependent Clp protease proteolytic subunit</fullName>
            <ecNumber evidence="5 8">3.4.21.92</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Endopeptidase Clp</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">clpP</name>
        <name evidence="11" type="ORF">BN566_01426</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 9">Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Fourteen ClpP subunits assemble into 2 heptameric rings which stack back to back to give a disk-like structure with a central cavity, resembling the structure of eukaryotic proteasomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 10">Belongs to the peptidase S14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.92" evidence="5 8"/>
    <dbReference type="EMBL" id="CBAR010000153">
        <property type="protein sequence ID" value="CCZ83690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01426"/>
    <dbReference type="EnsemblBacteria" id="CCZ83690">
        <property type="protein sequence ID" value="CCZ83690"/>
        <property type="gene ID" value="BN566_01426"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07017">
        <property type="entry name" value="S14_ClpP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00444">
        <property type="entry name" value="ClpP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001907">
        <property type="entry name" value="ClpP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023562">
        <property type="entry name" value="ClpP/TepA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033135">
        <property type="entry name" value="ClpP_His_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018215">
        <property type="entry name" value="ClpP_Ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10381">
        <property type="entry name" value="PTHR10381"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00574">
        <property type="entry name" value="CLP_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00127">
        <property type="entry name" value="CLPPROTEASEP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00493">
        <property type="entry name" value="clpP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00382">
        <property type="entry name" value="CLP_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00381">
        <property type="entry name" value="CLP_PROTEASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5 8" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 8" id="KW-0720">Serine protease</keyword>
    <feature type="active site" evidence="6">
        <location>
            <position position="99"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="99"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 7">
        <location>
            <position position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ83690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="196" mass="21742" checksum="BCFE9143F633699F" modified="2013-07-24" version="1">MSNLVPYVVEQTSRGERSYDIFSRLLNDRIIMLCDQVNDATASLVVAQLLYLESQDSEKDISLYINSPGGSITAGMAIYDTMQYIKCDVSTICIGMAASMGAFLLSSGAKGKRISLPNSEIMIHQPLISGGLSGQCTDIKIQSDHLVRIRQRMNEILAANTGKPIETIERDTERDNYMYAQEALEYGLIDKVMTNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0H9</accession>
    <name>R5V0H9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Endoribonuclease YbeY</fullName>
            <ecNumber evidence="7">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ybeY</name>
        <name evidence="8" type="ORF">BN566_01819</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Single strand-specific metallo-endoribonuclease involved in late-stage 70S ribosome quality control and in maturation of the 3' terminus of the 16S rRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the endoribonuclease YbeY family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="7"/>
    <dbReference type="EMBL" id="CBAR010000186">
        <property type="protein sequence ID" value="CCZ84093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01819"/>
    <dbReference type="EnsemblBacteria" id="CCZ84093">
        <property type="protein sequence ID" value="CCZ84093"/>
        <property type="gene ID" value="BN566_01819"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00009">
        <property type="entry name" value="Endoribonucl_YbeY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023091">
        <property type="entry name" value="MetalPrtase_cat_dom_sf_prd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002036">
        <property type="entry name" value="YbeY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020549">
        <property type="entry name" value="YbeY_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46986">
        <property type="entry name" value="PTHR46986"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02130">
        <property type="entry name" value="YbeY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00043">
        <property type="entry name" value="TIGR00043"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01306">
        <property type="entry name" value="UPF0054"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="7" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6 7" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="7">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ84093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="166" mass="18870" checksum="2F39EE93248EAE13" modified="2013-07-24" version="1">MMRVKVMVKNNQKTIKVPVGIRMLIRRCCQAVLVMEQFPHDAEVSVSFVSNAEIRNLNRIYRKKDSVTDVLSFPLGVDGKYDISKETGCALLGDVVISLETAMRQADLYGHSLEREIGFLTVHSMLHLLGYDHETSPLEARKMREKEEQVLGELGISREETFVSEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0J3</accession>
    <name>R5V0J3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01834</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000187">
        <property type="protein sequence ID" value="CCZ84108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84108">
        <property type="protein sequence ID" value="CCZ84108"/>
        <property type="gene ID" value="BN566_01834"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="54" mass="6411" checksum="D730716A9E399395" modified="2013-07-24" version="1">MPFPDLQTLLQASNSSRQFLLSLPVDVQLQLHEQNEWIHTQADLRRYAAYYHIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0Q9</accession>
    <name>R5V0Q9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01590</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000167">
        <property type="protein sequence ID" value="CCZ83855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83855">
        <property type="protein sequence ID" value="CCZ83855"/>
        <property type="gene ID" value="BN566_01590"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="79" mass="8836" checksum="AEC61F3E5BF377A0" modified="2013-07-24" version="1">MKLPPSKMDGLLEMVGKKLGVSPEQLRSDLESGKLEQAMQNLPKKEAANVQNLLKDQKKVEQLMNTPQAKALYEKLMGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0S6</accession>
    <name>R5V0S6_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glft2_N domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01928</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01928"/>
    <dbReference type="EnsemblBacteria" id="CCZ84203">
        <property type="protein sequence ID" value="CCZ84203"/>
        <property type="gene ID" value="BN566_01928"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040492">
        <property type="entry name" value="GlfT2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17994">
        <property type="entry name" value="Glft2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glft2_N" evidence="1">
        <location>
            <begin position="43"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="636" mass="75441" checksum="622D46F9D645B7E4" modified="2013-07-24" version="1">MRNVKFKIQNLIFRYNLQLEEHWWMMYRGARMQYDPLTSSQYLNPGDFVEFFTYFNSFSLGKWKKYTNVGDIYLHLRCKGHFKLQMFGHYREGRDIKKEMYEPVIYDLKELTDIEMMVPKDSKGQVIGFQIDVLPMTPEERKKTEIAEKKGVKAPDFRFQVESGSWETVVPEEKINDVRISIATTTFKKEDYITRNIGILERELFYSDEPAKNHIRLRIIDNGRTLDPDEFNSEYIHVYPNENVGGAGGFTRGILESISAEDFKATHVLLMDDDVMVLPESFIRTYSLLALVKPQYSERYVSGAMLYFEQMNLQHEDVGYVHDDGSYGPNKRIMEMHRWDCVFENDEDVDFHADSYAGWWYCCIPVKKIDRSHLPVPLFIRGDDVEFSVANHAEFLTLNGICIWHKGFANKFNANLELYMVHRNSLIIQAMSGICKDIDFIKRIQGFFETEIRRLAYNNCDLLLDAVEEFCAGPDFMKTPQGEQIMKSHAAKNEKMRPAAMVYSKPIDFDRVYRKEEKQLTPTQKWWYEVTDNGQKLPDWFLKKDQTAIIAYDWFDDPTKEYFAEQILAVSPFDHTAYLRKRDKQRYQQLKQRYQRVMRYYKQNRKQIEQMYQQAAGTLQSESFWREYLHMPEAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0S7</accession>
    <name>R5V0S7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">50S ribosomal protein L33</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpmG</name>
        <name evidence="5" type="ORF">BN566_01604</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the bacterial ribosomal protein bL33 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000169">
        <property type="protein sequence ID" value="CCZ83875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01604"/>
    <dbReference type="EnsemblBacteria" id="CCZ83875">
        <property type="protein sequence ID" value="CCZ83875"/>
        <property type="gene ID" value="BN566_01604"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00294">
        <property type="entry name" value="Ribosomal_L33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038584">
        <property type="entry name" value="L33_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001705">
        <property type="entry name" value="Ribosomal_L33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018264">
        <property type="entry name" value="Ribosomal_L33_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43168">
        <property type="entry name" value="PTHR43168"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00471">
        <property type="entry name" value="Ribosomal_L33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="SSF57829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01023">
        <property type="entry name" value="rpmG_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00582">
        <property type="entry name" value="RIBOSOMAL_L33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="49" mass="6059" checksum="D9C2B9D1DF00DA92" modified="2013-07-24" version="1">MRVKVTLACTECKRRNYVTKKNKRNNPERIEMNKHCKFCRKHTLHRETK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0U2</accession>
    <name>R5V0U2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metal-dependent membrane protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01619</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000169">
        <property type="protein sequence ID" value="CCZ83890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83890">
        <property type="protein sequence ID" value="CCZ83890"/>
        <property type="gene ID" value="BN566_01619"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071586">
        <property type="term" value="P:CAAX-box protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="176"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="198"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="260"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="307"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="333" mass="37301" checksum="5909CABED12E548B" modified="2013-07-24" version="1">MERTLKKAIRTASNVNSLMLLLFYGLIYAAVFGIQLLIPHLLPEDSTYYEAIYEVIVYFVQYVVIVPVLLFLFRAILGRKIGWNLKDSYRKPQASGGQLFRWCLIALGLVYATSMASQIFFNVIEMVTDVELQAPSMVAEENWVGYLNNIVAFSLYAPIFEEMLFRATLFRNTERFGSWFGVITIGITFGLWHCNYEQFFYTAVLGICAAFLTAKTRSVLPAMAIHFTMNFIGTMQSIAYSGLDTDNLDLEGMLQHPLKMLLLAGMNFLVIGILIAGCVLFIVELVKHRETFRLGNTVPQASGGKKALVYWTAPVTIVCVIVSLAMAIVNAIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0V5</accession>
    <name>R5V0V5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01958</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84233">
        <property type="protein sequence ID" value="CCZ84233"/>
        <property type="gene ID" value="BN566_01958"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="172" mass="19778" checksum="EEE2E7955719DCC1" modified="2013-07-24" version="1">MIKNLKADENNWFLEGTFDSDSLFQGEMSVSIFTDDGATVEDAEACIVQYQQLKKNAAFCANLQEMLAAYFLYMYDAWKEMEIYDEIVVEIEPVKQGYEAGENLLSYLSNPTLLVYPQEMDEIGYGIECDCPWEPEHSCLILVRNEKLLYVGESDGLDPWGEAEDYHCIWES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0X6</accession>
    <name>R5V0X6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00187</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000202">
        <property type="protein sequence ID" value="CCZ84258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84258">
        <property type="protein sequence ID" value="CCZ84258"/>
        <property type="gene ID" value="BN566_00187"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016621">
        <property type="entry name" value="UCP014543"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12646">
        <property type="entry name" value="DUF3783"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="125" mass="14243" checksum="59FB5C23D01C8D51" modified="2013-07-24" version="1">MEKLLLIQITPEDAILNLAQRMHIRTVSVSPNDYQKSIGQLAGFSDQTATHTSDGTGLDGSMLIFCNVKERHQNQILEKLRTMDVSITYKAILTPTNRHWTVFQLYDELHREHCAMAGKEQDDSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5V124</accession>
    <name>R5V124_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Stage III sporulation protein AC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01694</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000177">
        <property type="protein sequence ID" value="CCZ83965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01694"/>
    <dbReference type="EnsemblBacteria" id="CCZ83965">
        <property type="protein sequence ID" value="CCZ83965"/>
        <property type="gene ID" value="BN566_01694"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009570">
        <property type="entry name" value="Spore_III_AC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025664">
        <property type="entry name" value="Spore_III_AC/AD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06686">
        <property type="entry name" value="SpoIIIAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02848">
        <property type="entry name" value="spore_III_AC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="64" mass="6914" checksum="3F1553FD5CD1D5B0" modified="2013-07-24" version="1">MDIDLIFKIAGTGIIVAVLNLVLKRAEREEQAMMTTLAGLIVVLVIIVQEIGGLFETVKSVFGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5V130</accession>
    <name>R5V130_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01699</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000177">
        <property type="protein sequence ID" value="CCZ83970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01699"/>
    <dbReference type="EnsemblBacteria" id="CCZ83970">
        <property type="protein sequence ID" value="CCZ83970"/>
        <property type="gene ID" value="BN566_01699"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024232">
        <property type="entry name" value="SpoIIIAH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12685">
        <property type="entry name" value="SpoIIIAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="159" mass="17288" checksum="AECE52CDB2876A03" modified="2013-07-24" version="1">MKKPTLIIGKKQVILSCLTLMLAVAVYVNYSMSKDDRNLTTAESGVHTYGETEFVNAKAAKTTEVATDAAQEETTAVQENAPASFQPDTEAGAAAMEQLNADDYFAQARLERTSSRDEAVATLQSIMGGGDRTEDELVTDXXQKMNWSPMQLQPLKLPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5V145</accession>
    <name>R5V145_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">CoA-substrate-specific enzyme activase putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_00226</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FldB/FldC dehydratase alpha/beta subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000212">
        <property type="protein sequence ID" value="CCZ84313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00226"/>
    <dbReference type="EnsemblBacteria" id="CCZ84313">
        <property type="protein sequence ID" value="CCZ84313"/>
        <property type="gene ID" value="BN566_00226"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016836">
        <property type="term" value="F:hydro-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002731">
        <property type="entry name" value="ATPase_BadF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018709">
        <property type="entry name" value="CoA_activase_DUF2229"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008275">
        <property type="entry name" value="CoA_E_activase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010327">
        <property type="entry name" value="FldB/FldC_alpha/beta"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01869">
        <property type="entry name" value="BcrAD_BadFG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09989">
        <property type="entry name" value="DUF2229"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06050">
        <property type="entry name" value="HGD-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00241">
        <property type="entry name" value="CoA_E_activ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BcrAD_BadFG" evidence="5">
        <location>
            <begin position="14"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="BcrAD_BadFG" evidence="5">
        <location>
            <begin position="329"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2229" evidence="6">
        <location>
            <begin position="674"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="982"/>
            <end position="1005"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF09989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ84313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="1005" mass="109968" checksum="4AF4CE9448E6D5D8" modified="2013-07-24" version="1">MSEIKLQQKAAYTLGIDVGSTTVKTVILDADHHVLHSTYQRHFSKVKEAVSEQLQAIIKEHPATLFSVHMTGSAGLGLATAAKIPFVQEVHAAFLAVKERYPEADAVIELGGEDAKIIFLTGGVEQRMNGSCAGGTGAFIDQMATLLDVTVDELDQMALQADRTYPIAARCGVFAKSDIQPLLNQGAKRENVAASIYQAVVDQTVSGLAQGRKIQGHVLFLGGPLSFLQGLRNAFMQTLHLDAEHAIFPVDGRTFVAYGCALAAENSADKPVTGEALLKQIESAETNHTVIADKPLFGYQEEYDRFRERHAQNDVVHGDLHTYQGNAYLGVDAGSTTTKVALIGEDNSLLYSFYETNKGQPLECVKRALETLYQKKNPNLKICGSAVTGYGEEMLKAGLGIDFGIVETVAHFRAAEFFCPDVDFIIDIGGQDIKCFQIKNGAIDSIMLNEACSSGCGSFIQAFANALGYDVAEFAKLGLFAERPVDLGSRCTVFMNSSVKQAQKDGATVADISAGLSGSIVKNAIYKVIRAKSPEDLGKHIVVQGGTFLNDAVLRCFEREMGRDVIRPAISGLMGAFGAAIYAKEHTSGSSTLLSLMDLHTLTYRTQNTTCNGCTAHCNLQILRFSNGKRFIAGNRCERGAGVSKKDQIPNLVDWKYETLCSLTDRQPEQPKATVGLPMALHYYDQLPLWYTFFTDLGFAVKLSEESTRKTYYKGQSTIPSDTVCYPAKLAHGHIESLLEQNVDFIFYPCMTYNLDEGDSDNHYNCPVVAYYPELLLANNEKLNEQNFLHPFLDLNRRKHLITVLYACLSDYATKKEIAAALEHGFAALEQYHNQITQTAQEIVRQARAAGKTILVLAGRPYHIDPEINHGIHKLISSLGMAVITEDSIAGLAHTPELQALNQWTYHSRMYRAAQYVTTQPDFQLVQLVSFGCGIDAITTDEVRRILEQSGKLYTQLKIDEISNLGAAKIRLRSLKAAIGADVPPKPAAAPSEVAPKNEKEPVSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>R5V187</accession>
    <name>R5V187_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Imidazoleglycerol-phosphate dehydratase</fullName>
            <shortName evidence="5">IGPD</shortName>
            <ecNumber evidence="5 6">4.2.1.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">hisB</name>
        <name evidence="7" type="ORF">BN566_01729</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:11040"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57766"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="EC" id="4.2.1.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 5 6">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 6/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the imidazoleglycerol-phosphate dehydratase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.19" evidence="5 6"/>
    <dbReference type="EMBL" id="CBAR010000178">
        <property type="protein sequence ID" value="CCZ84000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01729"/>
    <dbReference type="EnsemblBacteria" id="CCZ84000">
        <property type="protein sequence ID" value="CCZ84000"/>
        <property type="gene ID" value="BN566_01729"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00011"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004424">
        <property type="term" value="F:imidazoleglycerol-phosphate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07914">
        <property type="entry name" value="IGPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00076">
        <property type="entry name" value="HisB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038494">
        <property type="entry name" value="IGPD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000807">
        <property type="entry name" value="ImidazoleglycerolP_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020565">
        <property type="entry name" value="ImidazoleglycerP_deHydtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23133">
        <property type="entry name" value="PTHR23133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00475">
        <property type="entry name" value="IGPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00954">
        <property type="entry name" value="IGP_DEHYDRATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00955">
        <property type="entry name" value="IGP_DEHYDRATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5 6" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="4 5 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ84000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="193" mass="20879" checksum="E9C134FCB980C238" modified="2013-07-24" version="1">MREAVIERNTKETQIQVKVVLDGKGVANIHTGIGFFDHMLTALAVHSGISMHIQVAGDLHVDGHHTVEDTGIVLGKAIGEALGDKSGITRYGSAFIPMDESLAFCSLDISNRPFLVFQGTFTNAMIGQYDTCLTEEFFRAFAFQAGITLHINMMYGTNDHHKCEAAFKAVAHALQIAITPTKSGQTLSTKGVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1B8</accession>
    <name>R5V1B8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Predicted membrane protein involved in D-alanine export</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN566_00285</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the membrane-bound acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000222">
        <property type="protein sequence ID" value="CCZ84378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00285"/>
    <dbReference type="EnsemblBacteria" id="CCZ84378">
        <property type="protein sequence ID" value="CCZ84378"/>
        <property type="gene ID" value="BN566_00285"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042121">
        <property type="term" value="P:alginic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024194">
        <property type="entry name" value="Ac/AlaTfrase_AlgI/DltB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028362">
        <property type="entry name" value="AlgI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500217">
        <property type="entry name" value="AlgI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016636">
        <property type="entry name" value="AlgI_DltB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="30"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="104"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="302"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="323"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="352"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="400"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="430"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR016636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ84378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="467" mass="53688" checksum="C79E136EADE6F4E5" modified="2013-07-24" version="1">MVFSSLFFLYLFLPLSLIAYYVAKPRYRNAVLIVCSLVFYAWGEPIFIWLLLMSTLLNYLFGRLISADPESRTAKVSLVLSLVFHLGMLILFKYTGFFIENLNNWFSLSIPVPNFPLPIGISFYTFRAVSYIVDCYWGKVEAEQKFSRFLLYMTFFPVIMQGPIARYETMQNQLAVRRCNPSDLYEGMMRVIIGLGKKVIIADNLGNIVDQFLANGNIEQQTTLGAWYGVIVYAMQIYFDFSGYSDMAIGIARMMGFQLEENFRHPFLCKDITEFWQRWHISLGTFFRDYVLYIPIFGKRRKYGGLFLVWFCTGLWHGASWNFILWGLYYGLFILIELLIGKKRIKKIPLVIRHLYNKIVIVVGFGIFYFEDFGQLGACLKCMFGFGGHGLTDAVLQTSIMTNVFLLVIALIGCFPILELPKRAMQKSPGAYLTLSVCGTILAAVILIVSSILLVNATNRPFLYLRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1C9</accession>
    <name>R5V1C9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">UPF0210 protein RUM_06210</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01771</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000181">
        <property type="protein sequence ID" value="CCZ84045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01771"/>
    <dbReference type="EnsemblBacteria" id="CCZ84045">
        <property type="protein sequence ID" value="CCZ84045"/>
        <property type="gene ID" value="BN566_01771"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007841">
        <property type="entry name" value="UPF0210"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37560">
        <property type="entry name" value="PTHR37560"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05167">
        <property type="entry name" value="DUF711"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="200" mass="20192" checksum="B91AA170F2AFBE13" modified="2013-07-24" version="1">MGQLVGKRASEMLGVEFGIVDLSLAPTPAVGDSVAHILEAMGLEICGTHGTTAALAMLNDAVKKGGVMASSSVGGLSGAFIPVSEDAGMIDAAVDGTLTLSKLEAMTAVCSVGLDMIAIPGDVDPTTISAIIADEMAIGMVNTKTTAVRVIPAIGMKEGDMLEFGGLFGSAPVMPVSKKSSARFINRGGKIPAPMQSLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1E6</accession>
    <name>R5V1E6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Colicin immunity protein/pyocin immunity protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01796</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the colicins ColE2/ColE8/ColE9 and pyocins S1/S2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000186">
        <property type="protein sequence ID" value="CCZ84070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84070">
        <property type="protein sequence ID" value="CCZ84070"/>
        <property type="gene ID" value="BN566_01796"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015643">
        <property type="term" value="F:toxic substance binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030153">
        <property type="term" value="P:bacteriocin immunity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035900">
        <property type="entry name" value="Colicin_E_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000290">
        <property type="entry name" value="Colicin_pyocin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01320">
        <property type="entry name" value="Colicin_Pyocin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47345">
        <property type="entry name" value="SSF47345"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0079">Bacteriocin immunity</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="205" mass="24307" checksum="23F421161DC90EE8" modified="2013-07-24" version="1">MMMYDKQELIKMVQRVIDCEEDEDTIDELLEILDDNLPHPSISDLIYWPPNEEELSAEEMIDIAISYQWKEHQKKCYSSSMKKLIAACKFDKNTSIEMKDILPKDFILSVKPVYSKADIREEVKNHTLNLYDILCSNDFEKQLQVVESLENWLKDSFPNKMFCSYFLYSETMASKFCFHMECPNMDWLSDKKVKSSNDCIRKNIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1F6</accession>
    <name>R5V1F6_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cysteine--tRNA ligase</fullName>
            <ecNumber evidence="11">6.1.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Cysteinyl-tRNA synthetase</fullName>
            <shortName evidence="11">CysRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">cysS</name>
        <name evidence="13" type="ORF">BN566_00315</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys)</text>
            <dbReference type="Rhea" id="RHEA:17773"/>
            <dbReference type="Rhea" id="RHEA-COMP:9661"/>
            <dbReference type="Rhea" id="RHEA-COMP:9679"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78517"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 11">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.16" evidence="11"/>
    <dbReference type="EMBL" id="CBAR010000224">
        <property type="protein sequence ID" value="CCZ84408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00315"/>
    <dbReference type="EnsemblBacteria" id="CCZ84408">
        <property type="protein sequence ID" value="CCZ84408"/>
        <property type="gene ID" value="BN566_00315"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004817">
        <property type="term" value="F:cysteine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006423">
        <property type="term" value="P:cysteinyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00672">
        <property type="entry name" value="CysRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00041">
        <property type="entry name" value="Cys_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015803">
        <property type="entry name" value="Cys-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015273">
        <property type="entry name" value="Cys-tRNA-synt_Ia_DALR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024909">
        <property type="entry name" value="Cys-tRNA/MSH_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032678">
        <property type="entry name" value="tRNA-synt_1_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890">
        <property type="entry name" value="PTHR10890"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09190">
        <property type="entry name" value="DALR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01406">
        <property type="entry name" value="tRNA-synt_1e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00983">
        <property type="entry name" value="TRNASYNTHCYS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00840">
        <property type="entry name" value="DALR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00435">
        <property type="entry name" value="cysS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 11 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="DALR_2" evidence="12">
        <location>
            <begin position="350"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="11">
        <location>
            <begin position="29"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="11">
        <location>
            <begin position="264"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CCZ84408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="464" mass="52698" checksum="5C29E6D91848FD4A" modified="2013-07-24" version="1">MHIYNTLTRKKEELVPIEPNHIRIYACGPTVYNYIHIGNARPICAFDVLRRYLKYRGYEVTYVQNFTDVDDKIIRRANEEGVPASELSERYIQEYKTDAHGLNVMDADIHPKVTENMDLIIDMVKKLVESGHAYESNGDVYFRTTSFSDYGKLSGQPMEELQAGARIDVNDQKENPLDFAVWKAAKPGEPYWESPWGKGRPGWHIECSAMSTHYLGDTLDLHCGGQDLIFPHHENEIAQSEAATGKAFSHYWMHNGYINIDNKKMSKSLGNFFTVREVAEQYGYEVIRYMMIQAHYRSPINYCKELLDACKASLERLYTCRNTLDRAIAAAPAGDCSEAAKELFAQREQQFITAMDDDLNTADGITALFELARELNRMSADATTPKGQLEAGAKLFDSLTDVLGILYQRKQEDAIPAEVQTLIEERAAARKAKNFAEADRLRDAIAALGYRVKETRQGTQIEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1K5</accession>
    <name>R5V1K5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_00350</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000229">
        <property type="protein sequence ID" value="CCZ84443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00350"/>
    <dbReference type="EnsemblBacteria" id="CCZ84443">
        <property type="protein sequence ID" value="CCZ84443"/>
        <property type="gene ID" value="BN566_00350"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025874">
        <property type="entry name" value="DZR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026870">
        <property type="entry name" value="Zinc_ribbon_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12773">
        <property type="entry name" value="DZR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13240">
        <property type="entry name" value="zinc_ribbon_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="zinc_ribbon_2" evidence="6">
        <location>
            <begin position="107"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="DZANK-type" evidence="5">
        <location>
            <begin position="143"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="65"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ84443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="204" mass="22188" checksum="F9B1F72D58AD0DF6" modified="2013-07-24" version="1">MPFFEDIGKKISNMGQTAVEKTKCSTETLRLNNQIREEQRLCKDLYQQIGQQYAQQYETNPDPAFESLMKQLHDAQERITSYQEQIQIKKAAVSQPQQSAYSTGCCCASCGAPLPPDAKFCTTCGAKVELTPPEPVETPSRVCPKCGNQLASDALFCASCGAKFEEPTAAVPPQEATAPTTCPNCGQSLSPDTQFCPICGEKTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1P8</accession>
    <name>R5V1P8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nitroreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00372</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000238">
        <property type="protein sequence ID" value="CCZ84473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00372"/>
    <dbReference type="EnsemblBacteria" id="CCZ84473">
        <property type="protein sequence ID" value="CCZ84473"/>
        <property type="gene ID" value="BN566_00372"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.109.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029479">
        <property type="entry name" value="Nitroreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000415">
        <property type="entry name" value="Nitroreductase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00881">
        <property type="entry name" value="Nitroreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55469">
        <property type="entry name" value="SSF55469"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitroreductase" evidence="1">
        <location>
            <begin position="8"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="174" mass="20003" checksum="D39DF5850B3215E0" modified="2013-07-24" version="1">MSFLELAKKRCSCRNFNDQQVPEETLQYILEAGRVAPTGVNKQPQHILVVRSKEGLAKMKQVTKFYNAQTVLIICTEPDAAWTRQGDDFNIADIDATIVTSHMILAATEKDVASLWICRFWPEIARKEFHIPDNYRPVNILALGYTDDAWKSPERHDTEELRKPLSETVTYETF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1R2</accession>
    <name>R5V1R2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01950</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01950"/>
    <dbReference type="EnsemblBacteria" id="CCZ84225">
        <property type="protein sequence ID" value="CCZ84225"/>
        <property type="gene ID" value="BN566_01950"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043765">
        <property type="entry name" value="DUF5711"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18975">
        <property type="entry name" value="DUF5711"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="391" mass="43759" checksum="3786E23C95B3114D" modified="2013-07-24" version="1">MGSKANFDAVQKRKKQRTKNNLKKLLLFLLIAAFCGYLYLQRDAWIPKLEGIGTRYESVITQNDGTLADGNFPLSITKNADYRAAVVENDLFILNASYLYLYSLHGDLKETRQLAYSKPILKSNDYFALLYENGGTRFRVDRQSGNVYTKTMDQAIITGAISSTGVVALVTESDLYSCVLQIFDKNGKKLYTRNCIERLSDIAFNADSTGCVFTELSAVDGDVTATLKCIQFDTTDYTWESEPLTTLSLALSLTENNHACVIGDTTCAYYDDKGNLESQYDYGGTLENYAVDAGNAAVLIRDEEKRETKLVIFPGSASEPIEIVVNGDAKAVQVYGDRVYMMNAETVIAYNFSGKAVATVRLDSTYDSFLKKDNYLFLLRYDKIDRVDFKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1S0</accession>
    <name>R5V1S0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01960</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84235">
        <property type="protein sequence ID" value="CCZ84235"/>
        <property type="gene ID" value="BN566_01960"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="71" mass="8389" checksum="1BE2022A56FB0067" modified="2013-07-24" version="1">MMMCAYYLNGNKNKRCAFCKYWYDPTNQYIRPQNPKNGIWKFETTARCVCLKTNLEMGAGMQCSKFENKIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5V211</accession>
    <name>R5V211_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">pre-crRNA processing endonuclease</fullName>
            <ecNumber evidence="2">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00263</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRISPR-associated protein Cas5 family. Subtype I-C/Dvulg subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="2"/>
    <dbReference type="EMBL" id="CBAR010000218">
        <property type="protein sequence ID" value="CCZ84350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00263"/>
    <dbReference type="EnsemblBacteria" id="CCZ84350">
        <property type="protein sequence ID" value="CCZ84350"/>
        <property type="gene ID" value="BN566_00263"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043571">
        <property type="term" value="P:maintenance of CRISPR repeat elements"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021124">
        <property type="entry name" value="CRISPR-assoc_prot_Cas5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013422">
        <property type="entry name" value="CRISPR-assoc_prot_Cas5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010155">
        <property type="entry name" value="CRISPR-assoc_prot_Cas5d"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09704">
        <property type="entry name" value="Cas_Cas5d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029950">
        <property type="entry name" value="Cas_CT1134"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01876">
        <property type="entry name" value="cas_Cas5d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02593">
        <property type="entry name" value="CRISPR_cas5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="2" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR029950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="250" mass="29130" checksum="DC24216BA1ACDBCA" modified="2013-07-24" version="1">MSKPTHENIVEFKVYGEDALFSDVLTRTGMEKYSYPIPTYEALKGIMHSIYWKPTIVWYIDAVRVMNRIQTQRRGIRPIHYSGGNDLSYCTYLRDVCYHVRAHFEWNDNRPELEHDRNENKHHNIAKRMIERGGRRDIFLGTRECMGYVEPCAFEEGTGFYDHSGELPYGLFYHGITYADEAVLSEDKGQMTVRFWNPIMRNGVISFVRPEECTIKRHVREMEIKPFGEEHGNFKGLSEFLGGDDVALGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5V236</accession>
    <name>R5V236_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00287</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000222">
        <property type="protein sequence ID" value="CCZ84380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00287"/>
    <dbReference type="EnsemblBacteria" id="CCZ84380">
        <property type="protein sequence ID" value="CCZ84380"/>
        <property type="gene ID" value="BN566_00287"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025945">
        <property type="entry name" value="DHHW"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14286">
        <property type="entry name" value="DHHW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="440" mass="49475" checksum="E9D09C847DC2BC77" modified="2013-07-24" version="1">MICKECGTKLTPEQTTCPNCGALQQQAVPKKRKKSDTASQQNQPQSDEVQMQWQDRLILGATVLVTLAAIAWLIFMLMKPADGNDQKAETAAVETMPSTTAVTTMETTTTTVTTYVTIVTTMDDGAEPIEVVSGILWDHKRAINLFNGYAEQTEAYAEILNQYQKDLGDDVQCYSILAPTNASFYTPAAFQDSTLSSEKDCMDAAEKIFEGVIPIDAYGVLKEHTAEPIYARTDHHWFQLGAYYVARAFAEQADVPFADLTTYKKYVEDDFVGSMAYYTNDYPDLVNSPEEFVYYVPTNDIQTTYYDRDYANGYESDLILDPSAWDNSSYYMVFMCGDDKIVHIQTDCDNDRRLVIFKDSYGNALVPCLTSSFSEIYVCDMRYFNLNAIDFIQEEAKATDVLFCMNVFSANGPNGQDLEVVRTQNAAYDTSSWEDADTEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5V2W0</accession>
    <name>R5V2W0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_00463</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAE transporter (TC 2.A.81) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000257">
        <property type="protein sequence ID" value="CCZ84610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00463"/>
    <dbReference type="EnsemblBacteria" id="CCZ84610">
        <property type="protein sequence ID" value="CCZ84610"/>
        <property type="gene ID" value="BN566_00463"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006512">
        <property type="entry name" value="YidE_YbjL"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06826">
        <property type="entry name" value="Asp-Al_Ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01625">
        <property type="entry name" value="YidE_YbjL_dupl"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="119"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="237"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="263"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="303"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="330"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="388"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="Asp-Al_Ex" evidence="7">
        <location>
            <begin position="25"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Asp-Al_Ex" evidence="7">
        <location>
            <begin position="241"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF06826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ84610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="412" mass="43488" checksum="6264456A149D09E0" modified="2013-07-24" version="1">MKFELLEVAGTHIFSMKAMTLVLFFIFAIIVVGYLVGKISIKGVSLGSAAIFLVALVAGHFKGVAFQDIASWQESLPAVDHYFKLVQNLGLVLFVTSVGLIAGPSFFKNLKKNAKSYVALGGIIIITGCLVCAAITLLVPNINSSMSVGLFAGALTSTPAFAAAQEAVGEANPLYGNVAVGYAIAYPFGVIGVVLFVQIIPKILHANMDEERAKLVSIQSNDAVGGKQKKLFEMDKFGIAAFFLAVMLGLILGSFHIPLGKGSFNLGTTGGPLIIGLIFGHFGRIGKLSLKVDQHVLSLFQELGLILFLIGAGMDGGAEFVAVLKEYGALLFLWGALMTLIPMIVGFLFAKYVLKLSLFNNLGSICGGMTSTPALGTLIKTANTPNVASAYAATYPIALILVVLLAQFLTRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5V2X4</accession>
    <name>R5V2X4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00478</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000257">
        <property type="protein sequence ID" value="CCZ84625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00478"/>
    <dbReference type="EnsemblBacteria" id="CCZ84625">
        <property type="protein sequence ID" value="CCZ84625"/>
        <property type="gene ID" value="BN566_00478"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005229">
        <property type="entry name" value="CHP00255"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013551">
        <property type="entry name" value="DUF1732"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013527">
        <property type="entry name" value="YicC-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30636">
        <property type="entry name" value="PTHR30636"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08340">
        <property type="entry name" value="DUF1732"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03755">
        <property type="entry name" value="YicC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00255">
        <property type="entry name" value="TIGR00255"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YicC_N" evidence="1">
        <location>
            <begin position="1"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1732" evidence="2">
        <location>
            <begin position="207"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="291" mass="33120" checksum="90D2EE79EF597F71" modified="2013-07-24" version="1">MKSMTGFGRGQFTADGVDILAEIRSVNHRFLECTVRLPRAYSFLEEPIKKAVQQVAARGKIEVNLTMQLPESATNTVAVNVELANAYCQALQAANETLQLQDDLTLSTLLRLPDVCTIQKQPMQEEEIWSDTKIALEQALQRFIAMRETEGEKLRQDVLSRLQTITQHLTVVEAEAPTMQQNYYDRLYQKLQTLLEDQSIDAQRLVTEAAVFSEKVAIDEETVRLRSHLQQCADLLEAKEPVGRKLDFLVQEMNREVNTIGSKAQNLSITQLVVEMKSEIEKIREQIQNIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>R5V4H1</accession>
    <name>R5V4H1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">RNA polymerase sigma-70 factor ECF subfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_00787</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000036">
        <property type="protein sequence ID" value="CCZ82994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82994">
        <property type="protein sequence ID" value="CCZ82994"/>
        <property type="gene ID" value="BN566_00787"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="3">
        <location>
            <begin position="14"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="4">
        <location>
            <begin position="116"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ82994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="184" mass="21491" checksum="5F56B240CBE159B3" modified="2013-07-24" version="1">MVKSSQQKEKLHQLYALYEQPLYRIAYAILHQTEQAEDAVSDAFLKIIPHLDQIESPDSPKTKAFLIQVIRNTAINQYRKNAKDAERLTNIDDSTLQVPSPHNEIEQFLQQTEQQESLAQLLQGLSETDRTILFLRCEQEQSFREIAERLSMKEATVRKRFERARKAIQKQEERGISYVKKCSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5V4P1</accession>
    <name>R5V4P1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative macrolide efflux protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00113</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000046">
        <property type="protein sequence ID" value="CCZ83059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00113"/>
    <dbReference type="EnsemblBacteria" id="CCZ83059">
        <property type="protein sequence ID" value="CCZ83059"/>
        <property type="gene ID" value="BN566_00113"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="170"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="220"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="253"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="306"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="348"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="386"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="424" mass="46077" checksum="DD649E0F673885DF" modified="2013-07-24" version="1">MKSFYELRFFLILWLTQAFSSLGSAMTNFALVLWLYQDSGSALKTAMLTVCSYAPYVLLSIFAGVISDRWNKKAVMLVCDSVAAAGTLVIVLFLQLRLLAVWHLYLLNACNGLMNAVQSPASDVATTLLTPKKYYQKTSGLRSFSNSLVSMLTPVLATAFFAFGGLKAVIAFDLSTFAVAFLVLLLWIPIPEVPHTTEQTESCLQAAKAGLVYLRSNRGIFWLILFLSSINLIASMHQAALPARILPQQGETALGIVNSCVGFATLLGSLLAVFCPKPKNRATAICNCLLVAMSTENFLLAFGRNPVIWCIGSIFGWGLIPLMSANYDVLFRSSVPKEMQGRVYAVRNTLQFFTIPVGYFLGGFLVDDVLEPWMAATQSEWLRQLFGTGLGSGAAVLFAGLGVAGVAVCLIFRNSASLRALDSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5V4S9</accession>
    <name>R5V4S9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">LITAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00896</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000059">
        <property type="protein sequence ID" value="CCZ83109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83109">
        <property type="protein sequence ID" value="CCZ83109"/>
        <property type="gene ID" value="BN566_00896"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006629">
        <property type="entry name" value="LITAF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10601">
        <property type="entry name" value="zf-LITAF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="LITAF" evidence="2">
        <location>
            <begin position="6"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="109" mass="11375" checksum="102A2ECDCD741F9C" modified="2013-07-24" version="1">MSKNKNNFKCPKCGGTSYTEYNDKLRNGAIGAAVGAFLLGPIGLLAAGLGTGTDSTGRKCNNCGKKVPNQLSAQEKKARGAAGKSFGSIATNQFKNGYQQAMSQYSDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5V591</accession>
    <name>R5V591_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01075</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83294">
        <property type="protein sequence ID" value="CCZ83294"/>
        <property type="gene ID" value="BN566_01075"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026002">
        <property type="entry name" value="ATC_hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14196">
        <property type="entry name" value="ATC_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="130" mass="14765" checksum="5EE8615A16870B47" modified="2013-07-24" version="1">MNEKMLLTVPKPLLHAVGKSYLNGFRKKAEQHLQRQNQPNFPENDWLITYRNLDDNSFEIDIHRCGFITIAKKYGVEGMLPGICSVDFMISHYMGNGFSRTKTLGAGDECCNCHYELQGTCPLHAPKGMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5V5D8</accession>
    <name>R5V5D8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Type III restriction enzyme res subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01125</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000092">
        <property type="protein sequence ID" value="CCZ83349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01125"/>
    <dbReference type="EnsemblBacteria" id="CCZ83349">
        <property type="protein sequence ID" value="CCZ83349"/>
        <property type="gene ID" value="BN566_01125"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="ResIII" evidence="2">
        <location>
            <begin position="110"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="875" mass="101149" checksum="B658BD0E05D7B61D" modified="2013-07-24" version="1">MAKKTTAFAQEYAFYEQLWTFYVENRGKIRSRYNDLTRKFLAYNDKSENPDAFLREPQFHSLEMYVFIKEFLDNAHMYEIFDDWRNRRNRFSDSSYYSIHKDGQFRFIDLGDDQNEAIFKQMKKFKEDYPNYIYALTMGLGKTILIATCIFYEFLLAKKYPKDKRYCQNALVFAPDKTVLDSLHEIMTFDKTKVVPPEYASVLDSNIKFHFLEDTGTTLHTIDDSKFNIIISNNQKIILKKKHKAEKPTDLLFEGGSLLSTVYGDDEDDDDSDILDDSMLIDNQRFKKLCRLPQLGVYVDEAHHLFGANLEKELRSGTANKTTLRNTINELNKKAHIVACYNYTGTPYVNRQILPEVVYAYGLNESIAHGYLKDADPIAFENVKNEEFLKVVITKFWERYGGQTYEGLMPKLAIFAATVEEATKTVRPAVEKILSELDIPLSSILVNTGDTTVTKDEDIRNFRNLDVQGTEGSQKQFIILVEKGREGWNCRSLLGIALFRSPKSKVFVLQATMRCLRQLSDEQLTATVFLSKENYDILNDELQKNYNMEITDMGKTSKKKKIECHVRVMPPPRKLVVKRIWHEYKLTEKPYSEPIPFGLNELDTSKYTLTMIEKGSLRLNAHEKETNIDYIKDNVQYSEFTLVAEIARYLNASCLLIAKILRESPDGIDTIIEMVNKYNEILHDILIPTIFHALNSVEAVRHSDDVEITLLKAPKDAGYYVFSGKEGLVITKEHNNFTPAEILKSFHADTYCFDSEPEKECFLQYISSPLVKEVYFTGMFTSDQGDLSIQYYDPESGRIRKYYPDFLAKMTDGSYQLIEVKGDNMIDDPVVKAKQIAAEEMATASEMHYLMYAGSKLMKENVLDCPNQYFQKTII</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>R5V5F3</accession>
    <name>R5V5F3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3 4">Single-stranded DNA-binding protein</fullName>
            <shortName evidence="2">SSB</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_01140</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000093">
        <property type="protein sequence ID" value="CCZ83364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01140"/>
    <dbReference type="EnsemblBacteria" id="CCZ83364">
        <property type="protein sequence ID" value="CCZ83364"/>
        <property type="gene ID" value="BN566_01140"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04496">
        <property type="entry name" value="SSB_OBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00984">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000424">
        <property type="entry name" value="Primosome_PriB/ssb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011344">
        <property type="entry name" value="ssDNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10302">
        <property type="entry name" value="PTHR10302"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00436">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002070">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00621">
        <property type="entry name" value="ssb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50935">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="100"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR002070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ83364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="129" mass="14691" checksum="DD194EAC7607496E" modified="2013-07-24" version="1">MLNKFLVSGRMTADPEIKTIGENRLCEFTIACERPKRKGEETAETDFFPCTAWNRNADVIVDWFGKGDMVTLVGSVHNKRYEKDGQKRSAIEVKVEEIHFNGGKKRENTAPTDNGYDNPYAKTEDDPLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5V5K7</accession>
    <name>R5V5K7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cysteine protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01190</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000098">
        <property type="protein sequence ID" value="CCZ83414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01190"/>
    <dbReference type="EnsemblBacteria" id="CCZ83414">
        <property type="protein sequence ID" value="CCZ83414"/>
        <property type="gene ID" value="BN566_01190"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040528">
        <property type="entry name" value="Lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18560">
        <property type="entry name" value="Lectin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="SSF54001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004394885" evidence="1">
        <location>
            <begin position="32"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="domain" description="Pept_C1" evidence="2">
        <location>
            <begin position="85"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="669" mass="74036" checksum="210D466C6043FADE" modified="2013-07-24" version="1">MKFPYKGIFAFCTAVCLFAGSMPISKVAASAKTSPRKAVQADCVSDFADADSEDGYLPLQGVAASFENSDCEQSLYGSAAETDTVPESYDLRDKGIITSVKDQGSDGMCWAFATLGAAESHLLKYYETDLDNINDMDLSEEQVGYFLYTPDPQPLSPTYGDAIIQTKKGASGGNALHASFFLSTVGVQEEAYLPYHGCSGFHSEYQRKQAAYRMTGSETMLSVQTKSERETIKKRMMQNGGIYVAFHSDGANYYDNGTTYAYYQSDSSYYSANHAVLLVGWDDNYAKENFDPKEQPENNGAWLVKNSWGDGKLDDGYFWISYEDTSLGEYASFTFEPREDSGNIYYYDGAGYSVAYSFDSVANVFRAEEDETLSRVGFYQTSYNGNNPKYQIQVYRLSETATDPTDGELLLDTTGHSGGFGYQEITLPETVSLQKNERFSVVLSMKIKKNQTWQNGYLTIEEDFDANNYSMQFSAQPGQSYILDQGSTEWLDATQLTGEKGAFHNVNLHAIMLPKEQEMDTAQLQAIETCAKAANETDIAEVAATILELSKEETIPQGLLNRVTAALMGLLEEQGTITYPDYAYPHAKWGDINEDGVVDVEDAVLVLTTYAKKAAGRENSLRYWQLIQGDVNGTLDDLTVEDAVAILTYYAKKASGQEATFAERQNSVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>R5V5R4</accession>
    <name>R5V5R4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">Protein GrpE</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">HSP-70 cofactor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">grpE</name>
        <name evidence="7" type="ORF">BN566_01238</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 4">Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3 5">Belongs to the GrpE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000111">
        <property type="protein sequence ID" value="CCZ83464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01238"/>
    <dbReference type="EnsemblBacteria" id="CCZ83464">
        <property type="protein sequence ID" value="CCZ83464"/>
        <property type="gene ID" value="BN566_01238"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00446">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.22.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01151">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000740">
        <property type="entry name" value="GrpE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013805">
        <property type="entry name" value="GrpE_coiled_coil"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009012">
        <property type="entry name" value="GrpE_head"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21237">
        <property type="entry name" value="PTHR21237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01025">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00773">
        <property type="entry name" value="GRPEPROTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51064">
        <property type="entry name" value="SSF51064"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58014">
        <property type="entry name" value="SSF58014"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01071">
        <property type="entry name" value="GRPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0346">Stress response</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ83464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="209" mass="23388" checksum="85D81987EE57B3E5" modified="2013-07-24" version="1">MEPNTEKNQQAEAQETAQETAKTAEATAAEETTATESAEQPETAAAEAESTTEEDQHMKKKELKAALEKAEAALADHKDQHLRLMAEYQNYRNRTTEEKKKIYGDAKADCIKSLLTVVDTFERAMDAACSDETYKKGIEMTFSQLQKALEQMGVKEIAEVGVEFDPNLHNAIKQMDDTDFEENKVCQIYQKGYLLGDRLIRPAMVAVSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>R5V5R9</accession>
    <name>R5V5R9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN566_01243</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CBAR010000111">
        <property type="protein sequence ID" value="CCZ83469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01243"/>
    <dbReference type="EnsemblBacteria" id="CCZ83469">
        <property type="protein sequence ID" value="CCZ83469"/>
        <property type="gene ID" value="BN566_01243"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="191"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="10">
        <location>
            <begin position="214"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="9">
        <location>
            <begin position="276"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="487"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ83469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="510" mass="57884" checksum="EBFAB997B6B4873D" modified="2013-07-24" version="1">MKDRAKKSRRFHPLSLRLICTEILAMLLVTVITAILSIVWYKGSVFTTIDKNDRVMATNLVHHYDREKSATETSAEWIPDGYVQDLFDWTTDTSHTFYFLFDETGTCLICSGQVNCNRSEITLNLDILNDVQINGEYFSDTLAMLNPKEPRPMISNGSPVVFTEPNGTTTTYYLFSNTRTSVLDAFTSRTWIYGGLCWMALTTGLIGITLLFLNRSQKPLEQMMQVAQQCAKGNYTEHLNPGSFEEPSYEDMAYSINELAYAAENAENQRKQFVSNVSHELRTPMTIISGYVDGMLDGTVPKNKRMQYLSIVSQEMQRLKILISSMLNLTKFDNGTIEIHYQLFPIQDLAFRTILMFESRLEKRNITLEGLDSEPVQVWGDPDLIGQVIYNLFENATKFVNTGGTITFTFKDEPNSTSIAIRNTGAGISKEELPKIFERFYKSDDSRSHDKTGLGIGLDITKHIVRLHKAQISVSSEEGEYTEFLIRLPKKDAPAESPTENPEKQPKDAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5V6B7</accession>
    <name>R5V6B7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01405</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000148">
        <property type="protein sequence ID" value="CCZ83669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83669">
        <property type="protein sequence ID" value="CCZ83669"/>
        <property type="gene ID" value="BN566_01405"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="160" mass="18784" checksum="1D69A5B301590106" modified="2013-07-24" version="1">MNKVCKRCGQPLPDGWNFYNNKRTGKLTKWTPGQCCRPCYNAINVERRHKKRDAYKSNPQVKAKATCKEPIPLKRYDNIDNCYIYLAACIVRTTMAEYERALKKYNNTPESMHYIERLEGDLLSYYYSILVLQSLDLRRYCINKRKAYGIGVYTTAQDVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>R5V744</accession>
    <name>R5V744_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Adenine DNA glycosylase</fullName>
            <ecNumber evidence="3 12">3.2.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN566_01648</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Adenine glycosylase active on G-A mispairs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolyzes free adenine bases from 7,8-dihydro-8-oxoguanine:adenine mismatched double-stranded DNA, leaving an apurinic site.</text>
            <dbReference type="EC" id="3.2.2.31"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="12">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the Nth/MutY family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.31" evidence="3 12"/>
    <dbReference type="EMBL" id="CBAR010000173">
        <property type="protein sequence ID" value="CCZ83919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01648"/>
    <dbReference type="EnsemblBacteria" id="CCZ83919">
        <property type="protein sequence ID" value="CCZ83919"/>
        <property type="gene ID" value="BN566_01648"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000701">
        <property type="term" value="F:purine-specific mismatch base pair DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03431">
        <property type="entry name" value="DNA_Glycosylase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005760">
        <property type="entry name" value="A/G_AdeGlyc_MutY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044298">
        <property type="entry name" value="MIG/MutY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029119">
        <property type="entry name" value="MutY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42944">
        <property type="entry name" value="PTHR42944"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14815">
        <property type="entry name" value="NUDIX_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="SSF48150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01084">
        <property type="entry name" value="mutY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6 12" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="11 12" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 12" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENDO3c" evidence="13">
        <location>
            <begin position="67"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU365096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CCZ83919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="374" mass="42686" checksum="C7E9A22A219D08FF" modified="2013-07-24" version="1">MPELCQEPHMLPVYEKLIARMKQVRQEQQAILSQMVAPLLDWYAIHKRDLPWRKDQNPYHVWISEIMLQQTRVEAVKGYYQRFLTAFPTIQALAEAAPEQVRKCWEGLGYYTRAKNLQRAAQQILEQYHGEFPTKHEEVLSLAGIGAYTAGAICSICYEQPTPAVDGNVLRVVMRLQDAFDEIDRPDVKRAVTEALKTCYPAGKCGMFTQALMELGALVCVPNGAPHCQECPVAALCRSRKQETQALLPVRKKKAARRKEQRVVWILQCGERYAVCQRPESGLLAGLWEFPNQLGTMTEAEAIQQAKNWGCKPISIEKTAAKKHIFTHIEWKMFGVYLTCAAMPEMFVWKTAAELQAELTLPTAFRQFLQEELQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7H3</accession>
    <name>R5V7H3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glutamyl-tRNA reductase</fullName>
            <shortName evidence="4">GluTR</shortName>
            <ecNumber evidence="4">1.2.1.70</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hemA</name>
        <name evidence="11" type="ORF">BN566_01755</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>(S)-4-amino-5-oxopentanoate + NADP(+) + tRNA(Glu) = H(+) + L-glutamyl-tRNA(Glu) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:12344"/>
            <dbReference type="Rhea" id="RHEA-COMP:9663"/>
            <dbReference type="Rhea" id="RHEA-COMP:9680"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57501"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="EC" id="1.2.1.70"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="domain">
        <text evidence="4">Possesses an unusual extended V-shaped dimeric structure with each monomer consisting of three distinct domains arranged along a curved 'spinal' alpha-helix. The N-terminal catalytic domain specifically recognizes the glutamate moiety of the substrate. The second domain is the NADPH-binding domain, and the third C-terminal domain is responsible for dimerization.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="4">During catalysis, the active site Cys acts as a nucleophile attacking the alpha-carbonyl group of tRNA-bound glutamate with the formation of a thioester intermediate between enzyme and glutamate, and the concomitant release of tRNA(Glu). The thioester intermediate is finally reduced by direct hydride transfer from NADPH, to form the product GSA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glutamyl-tRNA reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.70" evidence="4"/>
    <dbReference type="EMBL" id="CBAR010000180">
        <property type="protein sequence ID" value="CCZ84029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01755"/>
    <dbReference type="EnsemblBacteria" id="CCZ84029">
        <property type="protein sequence ID" value="CCZ84029"/>
        <property type="gene ID" value="BN566_01755"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00316"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008883">
        <property type="term" value="F:glutamyl-tRNA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00087">
        <property type="entry name" value="Glu_tRNA_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000343">
        <property type="entry name" value="4pyrrol_synth_GluRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015895">
        <property type="entry name" value="4pyrrol_synth_GluRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036343">
        <property type="entry name" value="GluRdtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006151">
        <property type="entry name" value="Shikm_DH/Glu-tRNA_Rdtase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05201">
        <property type="entry name" value="GlutR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01488">
        <property type="entry name" value="Shikimate_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000445">
        <property type="entry name" value="4pyrrol_synth_GluRdtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69742">
        <property type="entry name" value="SSF69742"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4 7" id="KW-0521">NADP</keyword>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 4" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GlutR_N" evidence="10">
        <location>
            <begin position="7"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Shikimate_DH" evidence="9">
        <location>
            <begin position="174"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="4 6">
        <location>
            <begin position="47"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="4 6">
        <location>
            <begin position="109"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4 5">
        <location>
            <position position="48"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4 6">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 6">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 7">
        <location>
            <begin position="184"/>
            <end position="189"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for activity" evidence="4 8">
        <location>
            <position position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000445-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000445-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000445-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000445-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ84029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="404" mass="45124" checksum="C2A04EB658CC1A0B" modified="2013-07-24" version="1">MYLQMAGLDYHIASMEQREPFSFSKEQQETILKTLKDSVQGVVLLNTCNRTELYLSSDVPCQPSQWLLQFAKETHPSIESVPFYEKSGIAAARHIIEVACGLHSQVLHEEQIVTQVQQAVGQARQLQTTDAILDTLFRTAVSAGKYALTHISDTEIPVSLAEKAIQTIEQQGFSLSNKIGLVIGNGNMGQLAAQQLMKRDCTVYITLRAHHNSSSIVPFGAIPVPYEKRMQYVAQSDLVISATRSPHYTITAEQLQSIDKKPSYLVDLALPRDIELTCKEIPSVHCLDLDDFSDAAEQHQNMQAALQEVAEKYTADFQAWQQYRAVIPHIAVLKELVVANLLRSTTMDAVRTQPDADKVIQVAAERTIDLIMGNMREWITPEQVEHCCQKIASHSRLPQKGVTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7W9</accession>
    <name>R5V7W9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methyltrans_SAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_01944</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01944"/>
    <dbReference type="EnsemblBacteria" id="CCZ84219">
        <property type="protein sequence ID" value="CCZ84219"/>
        <property type="gene ID" value="BN566_01944"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019614">
        <property type="entry name" value="SAM-dep_methyl-trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10672">
        <property type="entry name" value="Methyltrans_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltrans_SAM" evidence="4">
        <location>
            <begin position="55"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ84219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="290" mass="32525" checksum="2A130A5F0A6C6EFE" modified="2013-07-24" version="1">MRIANQWNDYRILDTSGQEKLEQWGNVTLIRPDPQIIWKTPKEHGMWNHADGHYHRSNAGGGSWDFFRKLPESWNIQYTPLDLKFRIQPTSFKHTGLFPEQAVNWDFVSNLIQNAGRPISVLNLFAYTGGATLACARAGASVCHVDASKGMTQWARENAALSGLSDRPIRWIVDDCEKFVAREIRRNRRYDAIIMDPPSYGRGPGGEIWKLEDCIYDLVQLCAGVLTEQPLFFLINSYTTGLSPSVMGYILGSVVRKRFGGTVTFDEIGLPVAQSGLALPCGSTAIWQAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5V806</accession>
    <name>R5V806_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NADPH-dependent FMN reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00188</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000202">
        <property type="protein sequence ID" value="CCZ84259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00188"/>
    <dbReference type="EnsemblBacteria" id="CCZ84259">
        <property type="protein sequence ID" value="CCZ84259"/>
        <property type="gene ID" value="BN566_00188"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN_red" evidence="1">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="182" mass="20636" checksum="9BDC57930CF3A217" modified="2013-07-24" version="1">MKTLILNGSPRKNGDTAQLLELAKAQLQGTYHVVSAYTADIHPCIDCRHCQKQFDCIFADEMEAVYTYLKDCDRILLASPIYFSELTGSLLNVISRLQPYFYARYFRKEPIPLHIQKAVTVLVGGGTGSPKKAYDTATGIFQLLQVKEQYPFFCSHHTDQIPATADLDFMQTFQKAMHFLNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>R5V8E7</accession>
    <name>R5V8E7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Chaperone protein HtpG</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Heat shock protein HtpG</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">High temperature protein G</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">htpG</name>
        <name evidence="8" type="ORF">BN566_00301</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Molecular chaperone. Has ATPase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the heat shock protein 90 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000224">
        <property type="protein sequence ID" value="CCZ84394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00301"/>
    <dbReference type="EnsemblBacteria" id="CCZ84394">
        <property type="protein sequence ID" value="CCZ84394"/>
        <property type="gene ID" value="BN566_00301"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00505">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019805">
        <property type="entry name" value="Heat_shock_protein_90_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037196">
        <property type="entry name" value="HSP90_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001404">
        <property type="entry name" value="Hsp90_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020575">
        <property type="entry name" value="Hsp90_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11528">
        <property type="entry name" value="PTHR11528"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00183">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002583">
        <property type="entry name" value="Hsp90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00775">
        <property type="entry name" value="HEATSHOCK90"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110942">
        <property type="entry name" value="SSF110942"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00298">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="HATPase_c" evidence="7">
        <location>
            <begin position="24"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="A; substrate-binding" evidence="5">
        <location>
            <begin position="1"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="C" evidence="5">
        <location>
            <begin position="557"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="489"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ84394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="629" mass="72828" checksum="6F2CA53FF1CEF99C" modified="2013-07-24" version="1">MEQKQFKAESKRLLDLMIHSIYTHKEIFLRELISNASDAIDKLYFRSLTDDSVNMTKSDFAIEIIPDKENRTLTIKDNGIGMTKEELEEHLGTIANSGSLQFKSENQMEDDHQIIGQFGVGFYSAFMVASRVTVKSKAFGAEKAYVWQSEGTDGYTIDECDKDTVGTEITLELLPDEEDKEEGAEKYSDYLDQYRIQSLVKRYSDYIRFPIRMEMTKSRKKEDSDEYEEYTELVTLNSMTPLWKKNKNELTDDDYNNFYKDKFNDYSNPLHHALVHNEGTVTYDALLYIPERAPFNYYSKEYEKGLQLYANGVLIMERCEDLLPDYFSFVKGLVDSEDVSLNISREMLQHDAQMRLIAKSVERTIKNELSRMMKNEREQYEKFYQAFGLQLKYGVYSDYGMHKDMLQDLLLFTSSKDQKLTSLAEYVDRMPEDQTCIYYACGESIARIEALPQTELVKDKGYEILYFTDSVDEFAIRMLMKYKDKEFKSVSANDLNLESEEEKKELEQKETESKDMLETMQKALDGKVAKVKLSGRLKSHPVCLSNEGDLSMEMAKTLNAMPGVDQPVQAQTILELNPEHPIYQKLKTLDDEMLGKYSQLLYNQALLIEGMPIADPVAFSNLICELMEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>R5V8H9</accession>
    <name>R5V8H9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ATP synthase subunit b</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">ATP synthase F(0) sector subunit b</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">ATPase subunit I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">F-type ATPase subunit b</fullName>
            <shortName evidence="11">F-ATPase subunit b</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">atpF</name>
        <name evidence="14" type="ORF">BN566_00326</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the F(0) channel, it forms part of the peripheral stalk, linking F(1) to F(0).</text>
    </comment>
    <comment type="function">
        <text evidence="10 11">F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">F-type ATPases have 2 components, F(1) - the catalytic core - and F(0) - the membrane proton channel. F(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). F(0) has three main subunits: a(1), b(2) and c(10-14). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. F(1) is attached to F(0) by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell membrane</location>
            <topology evidence="11">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11 12">Belongs to the ATPase B chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000225">
        <property type="protein sequence ID" value="CCZ84419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00326"/>
    <dbReference type="EnsemblBacteria" id="CCZ84419">
        <property type="protein sequence ID" value="CCZ84419"/>
        <property type="gene ID" value="BN566_00326"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045263">
        <property type="term" value="C:proton-transporting ATP synthase complex, coupling factor F(o)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01398">
        <property type="entry name" value="ATP_synth_b_bprime"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028987">
        <property type="entry name" value="ATP_synth_B-like_membr_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002146">
        <property type="entry name" value="ATP_synth_b/b'su_bac/chlpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005864">
        <property type="entry name" value="ATP_synth_F0_bsu_bac"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00430">
        <property type="entry name" value="ATP-synt_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81573">
        <property type="entry name" value="SSF81573"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01144">
        <property type="entry name" value="ATP_synt_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3 11 12" id="KW-0138">CF(0)</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 11 12" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 11 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 11 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="42"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CCZ84419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="162" mass="18011" checksum="5023B6325F29089C" modified="2013-07-24" version="1">MLEFDIWTIAFSIINILVLFLFLKKFLFGRIQNIMDQRATAVQADLDQAKASKEEAHQLRQQYEDTLSGAKQEASTIIANARTAAKEQGNQITEQAQQEASRMIQNAQKEIDAERQNTLAGAQKEIADLALAAAAKLVEGKMDDAENRNLVDAFLKEEGVNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5V951</accession>
    <name>R5V951_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Serine/threonine protein phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00487</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000257">
        <property type="protein sequence ID" value="CCZ84634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00487"/>
    <dbReference type="EnsemblBacteria" id="CCZ84634">
        <property type="protein sequence ID" value="CCZ84634"/>
        <property type="gene ID" value="BN566_00487"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006470">
        <property type="term" value="P:protein dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PTHR13832"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="SSF81606"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="242" mass="26902" checksum="D65EAA0946E06593" modified="2013-07-24" version="1">MNFATATHIGYVREENQDCVEIRTFPDGVLALVCDGMGGERSGKEASTRATEIFLECMEERCQEPMQTYEIRNAMMAAVSAANSVIYTAAKLNYQNFGMGTTCVAAFWNQEGVTIVNVGDSRAYYYADGVLQPITIDHTLVNFLLFSGKITEEELETHPMKHMLIRAVGVEKTVEADDFFLPYTDGMKILLCSDGLHGFCTEEEMQEILQQEKPAQELADELLQLALEKGGRDNIAIAIITD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>R5V996</accession>
    <name>R5V996_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Acetylornithine aminotransferase</fullName>
            <shortName evidence="2">ACOAT</shortName>
            <ecNumber evidence="2">2.6.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">argD</name>
        <name evidence="4" type="ORF">BN566_00542</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>2-oxoglutarate + N(2)-acetyl-L-ornithine = L-glutamate + N-acetyl-L-glutamate 5-semialdehyde</text>
            <dbReference type="Rhea" id="RHEA:18049"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29123"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
            <dbReference type="EC" id="2.6.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
        <text evidence="2">Binds 1 pyridoxal phosphate per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 4/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="2">May also have succinyldiaminopimelate aminotransferase activity, thus carrying out the corresponding step in lysine biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. ArgD subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.11" evidence="2"/>
    <dbReference type="EMBL" id="CBAR010000267">
        <property type="protein sequence ID" value="CCZ84689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00542"/>
    <dbReference type="EnsemblBacteria" id="CCZ84689">
        <property type="protein sequence ID" value="CCZ84689"/>
        <property type="gene ID" value="BN566_00542"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00109"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003992">
        <property type="term" value="F:N2-acetyl-L-ornithine:2-oxoglutarate 5-aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01107">
        <property type="entry name" value="ArgD_aminotrans_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004636">
        <property type="entry name" value="AcOrn/SuccOrn_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00707">
        <property type="entry name" value="argD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 4" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="2" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 2 3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="2">
        <location>
            <begin position="102"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="2">
        <location>
            <begin position="220"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>N(2)-acetyl-L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>N(2)-acetyl-L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="2">
        <location>
            <position position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="389" mass="41883" checksum="A5F0F40C3CD5FFE0" modified="2013-07-24" version="1">MTTQEKQNQYIMGTYGRFPVVLEQGKQESCQDETGKQYIDFGSGIGTNSLGYCNEAWVNAVCKQAQTLQHTSNLYYTKAQADFAEALCKATGYHDLFFCNSGAEANECAIKLARKYSFDKYGKGRHKILTLVNSFHGRTMATLSATGQDVFHNYFFPFLEGFDYVVANDIADLDAKLDDTVCAILIEYVQGEGGVMALEQDFVDAIYDRCAKRDILVMTDEVQTGMGRTGTLLAGEQFGHHADVVTLAKGIAGGLPMGACLANEKCSGVLGKSAHGSTFGGNPVCCAGGLAVLQTLTADGFLASVQEKAAYLREKLAKLPHVQAVTGLGLMVGIQLDADKKAIDVVNAALEQGLLLLTAKDRVRLLPPLTISKDELDRGVAILADVLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VH19</accession>
    <name>R5VH19_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00666</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000012">
        <property type="protein sequence ID" value="CCZ82861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82861">
        <property type="protein sequence ID" value="CCZ82861"/>
        <property type="gene ID" value="BN566_00666"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ82861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="382" mass="41515" checksum="0B6B57DA9CEF63E9" modified="2013-07-24" version="1">MAVCFIPCRCCFIGRSISVTRSGSVVRIFRRIPIETNDQILTGGVVAQNHAHLCLAPVAVIVIGVLRNNHCNQLHCSVGSCTAPACPTRHPECALIGAVLAYIRICPFPVACNFAVQSCCTRFSIAPAKRCINFAPLTIASCEEIIHKALILVILGSQIQETSCPSLIIFISIRSIKTVCTVCCTLIILCGELVQCLCILQVFCIAEVQVIYCQSCCIFCCNIACTIACPECLTTTLNGFLCFRRTPVAVVIAAAAFANLIAEFRNRVTAQNSAVLEVLAEPIGDFFQVCSLTQRIICILEFIVAPAQCCCGITSRQTKLYVVTCIVETANDFIASVAIATPVKCRSRIYIIAAHISIVEIHGILQQFCVVVRVRRMNRQIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5VH63</accession>
    <name>R5VH63_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Endoglucanase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN566_00698</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000022">
        <property type="protein sequence ID" value="CCZ82896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00698"/>
    <dbReference type="EnsemblBacteria" id="CCZ82896">
        <property type="protein sequence ID" value="CCZ82896"/>
        <property type="gene ID" value="BN566_00698"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002105">
        <property type="entry name" value="Dockerin_1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018087">
        <property type="entry name" value="Glyco_hydro_5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00404">
        <property type="entry name" value="Dockerin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00659">
        <property type="entry name" value="GLYCOSYL_HYDROL_F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="1" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="3 5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004407052" evidence="7">
        <location>
            <begin position="23"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="8">
        <location>
            <begin position="534"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="494"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ82896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="604" mass="67362" checksum="E7DFC17B984F835E" modified="2013-07-24" version="1">MKMWKRWKAVLLAGMMACTAVAASVPAIGTLNAQAEEDTNNDDWLHAVGSRLYDKDGNEVWLTGANWFGMNCTENSPHGLYAADINVFVENIANHGINIVRMPISTELLCSWMNGEPLEVKSMNCYANPELSKNGDGKDPAKNSMEIFDIIMQKMKKYGIKVMVDVHSPAAHNSGHNYNLWYYDASAADADNMAVGAYSGEQITWDMWKDSITWLADKYKNDDTLIGYDLKNEPHGKRGYNGTTCPTDIAKWDDSTDLNNWAYSATECANSILDVNPNALIFVEGVEQYPKTEKGYTYDTADIWQAPADVSPWYGAWWGGNLRGVRDYPIQPDSGTSQIVYSPHDYGPSVYNQTWFDKDFTEQTLLDDYWYDTWAYINAENIAPLLIGEWGGHMDGGKNEKWMTLLRDYMINHHINHTFWGLNPNSGDTGGLLSYDFMTWDTEKYDMFKESLWQTQKTGKFIGLDHQKALGTDGSGISVSEFYKSYASTEGSNLDGGTIVDGNTTKPTTEPTKPSTTTTTTTTTTAAITTEAPITEVIGDIDGDKKLTVSDAVLLNRYLLRKIDGKDAAYTFERGDLNKDGVLNIVDAIAYKQYLSGNIAPPEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5VHQ3</accession>
    <name>R5VHQ3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Exonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00819</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000040">
        <property type="protein sequence ID" value="CCZ83026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00819"/>
    <dbReference type="EnsemblBacteria" id="CCZ83026">
        <property type="protein sequence ID" value="CCZ83026"/>
        <property type="gene ID" value="BN566_00819"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Exonuclease_RNaseT/DNA_pol3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exonuclease" evidence="1">
        <location>
            <begin position="4"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="221" mass="25389" checksum="6E84F210170637FF" modified="2013-07-24" version="1">MRGKKIVLDLEFIPYRRKQEPYCVHEIIEIGAVALDADNEIYDEFQMYVKPQYGTVPPFVTAITGITNEMLVGQVSFSEAMQAFEAWLPQKEPYRFYTWSGSDQHVFQAEAEWKERPLLGNFYGWWVDLQRLHQRIYGFSKPMKLVTALGSMQIYFEGTEHGALADARNTAKILQELSDVRRVKNNFSTSHVTYNIASDHTSGGFTIGRLPIRKTAHSGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>R5VHQ8</accession>
    <name>R5VHQ8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Recombination protein RecR</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">recR</name>
        <name evidence="9" type="ORF">BN566_00824</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">May play a role in DNA repair. It seems to be involved in an RecBC-independent recombinational process of DNA repair. It may act with RecF and RecO.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the RecR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000040">
        <property type="protein sequence ID" value="CCZ83031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00824"/>
    <dbReference type="EnsemblBacteria" id="CCZ83031">
        <property type="protein sequence ID" value="CCZ83031"/>
        <property type="gene ID" value="BN566_00824"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01025">
        <property type="entry name" value="TOPRIM_recR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00017">
        <property type="entry name" value="RecR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000093">
        <property type="entry name" value="DNA_Rcmb_RecR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023627">
        <property type="entry name" value="Rcmb_RecR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023628">
        <property type="entry name" value="Rcmb_RecR_C4-type_Zn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015967">
        <property type="entry name" value="Rcmb_RecR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034137">
        <property type="entry name" value="TOPRIM_RecR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30446">
        <property type="entry name" value="PTHR30446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02132">
        <property type="entry name" value="RecR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13662">
        <property type="entry name" value="Toprim_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111304">
        <property type="entry name" value="SSF111304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00615">
        <property type="entry name" value="recR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01300">
        <property type="entry name" value="RECR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5 7" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="6 7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="1 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Toprim" evidence="8">
        <location>
            <begin position="82"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="7">
        <location>
            <begin position="59"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ83031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="200" mass="22008" checksum="B0EAA2BEF8BEB9EA" modified="2013-07-24" version="1">MADSNALPMVLLAEQFAKLPGIGMKSAQRLAYYLISRPEEEVQAFADAMVHAKKAIHYCGCCQNLTDQVVCPICSDDTRDHGIICVVEAPKDVAAFERTNEYHGVYHVLHGLISPLTGVMPEQLKIRELLARLQSGTVREVIMATNPTVEGDATAMYLAQLLKPMQIKVSRLAFGLPVGGVVEYADEVTLYKALENRSEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5VI76</accession>
    <name>R5VI76_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein conserved in bacteria</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01042</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01042"/>
    <dbReference type="EnsemblBacteria" id="CCZ83261">
        <property type="protein sequence ID" value="CCZ83261"/>
        <property type="gene ID" value="BN566_01042"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007329">
        <property type="entry name" value="FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04205">
        <property type="entry name" value="FMN_bind"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00900">
        <property type="entry name" value="FMN_bind"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN_bind" evidence="2">
        <location>
            <begin position="93"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="FMN_bind" evidence="2">
        <location>
            <begin position="213"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="FMN_bind" evidence="2">
        <location>
            <begin position="320"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="FMN_bind" evidence="2">
        <location>
            <begin position="454"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="542" mass="55895" checksum="E7C20A196DBEBBFC" modified="2013-07-24" version="1">MQGKHTPWASLFGFLPAALLAAFVTISLHDYTAPIFSVEQEDTAVSTQETTANGKKSSSKTAAVTTKAAPTVEPLAEQTGGYKDGTYTGSGTGFSGLITVQVVIEGGKITDITIVSTTDDSPYIDNASALLKTIIATQSTNVDTVSGATYSSVGLISAVRDALQKAGGSSAGDSALPTLAATNSNASGDAPSISAVSEPSSYRDGTYVGTGSGFAGAITVQVTVSDGKISDISVLSASDDSPYLDNAMSLLNHIIALNSTNVDTVSGATFSSVGLIEAVRNALANAGADSGNNAEETTPVEVPKGRFPYPDGVYIGSGEGYRGETTVAVSLKDGTIDNIMVMNTEDDTAYFKRAESLLKQVLQQQTTDLDAISGATFSSEGILEAIADALEQAKQANSGNQTTTVTTAPAETTTTTVVTTKPVETTEPTPSLYIDGEYIGSASCYPDDDEDFLPYDLTVKVCIENDQIISITDVEGFGADYDSANDWYIDRALNGTKKISGIAAQILAAQTTDVDAVSGATCSSDAILAAVQDALQQALREG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJ54</accession>
    <name>R5VJ54_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01285</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000119">
        <property type="protein sequence ID" value="CCZ83516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83516">
        <property type="protein sequence ID" value="CCZ83516"/>
        <property type="gene ID" value="BN566_01285"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="78" mass="9183" checksum="63C92990B7A5D1C0" modified="2013-07-24" version="1">MKIPTLGEARNSVVYYSREKQDCIADLFYNYQNCTGKRKNFWNDLRESLVKSYKSSVVSRRFLWYNGKNCGKEGAFLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJ77</accession>
    <name>R5VJ77_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA(Met) cytidine acetate ligase</fullName>
            <ecNumber evidence="1">6.3.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tmcAL</name>
        <name evidence="3" type="ORF">BN566_01319</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of N(4)-acetylcytidine (ac(4)C) at the wobble position of elongator tRNA(Met), using acetate and ATP as substrates. First activates an acetate ion to form acetyladenylate (Ac-AMP) and then transfers the acetyl group to tRNA to form ac(4)C34.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetate + ATP + cytidine(34) in elongator tRNA(Met) = AMP + diphosphate + N(4)-acetylcytidine(34) in elongator tRNA(Met)</text>
            <dbReference type="Rhea" id="RHEA:58144"/>
            <dbReference type="Rhea" id="RHEA-COMP:10693"/>
            <dbReference type="Rhea" id="RHEA-COMP:10694"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:74900"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TmcAL family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.-" evidence="1"/>
    <dbReference type="EMBL" id="CBAR010000125">
        <property type="protein sequence ID" value="CCZ83551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01319"/>
    <dbReference type="EnsemblBacteria" id="CCZ83551">
        <property type="protein sequence ID" value="CCZ83551"/>
        <property type="gene ID" value="BN566_01319"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01539">
        <property type="entry name" value="TmcAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008513">
        <property type="entry name" value="tRNA(Met)_cyd_acetate_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37825">
        <property type="entry name" value="PTHR37825"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05636">
        <property type="entry name" value="HIGH_NTase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="1" id="KW-0820">tRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="396"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="7"/>
            <end position="20"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="416" mass="46481" checksum="8A72A8A17076CCB1" modified="2013-07-24" version="1">MKITGIICEYNPLHNGHLYQIQQVRKNGAEAIVAVMSGNFMQRGDVAVMDKFTRAKLAVESGVDLVIELPVPYAVAPAEVFALGGVALLSALPNVTEISFGSECGDLELLRQAADACYLCKTEYRDVMQDFLKQGHPYPEVLMHMVEQLYGTEVAEVLLEPNNTLAVEYLNAMHTLKSPLEPYTVQRRGAGHHSLLLGEERPEEGTAGIASATYIRNCIQNGVDCRRTVPEYCYQTLQEMEEAGQIADIHYLERILLYQLRTTSVENLRTIAEIGQGLEHRLYQARSATSLENLLEILQTKRYPLARLRRILIHVLLDIRKSDTKGLPPYGRILAFNDMGRQILRCSKDSRGIPFSHSLKELSKLNPAASRCASLDAKATDIFYLAMPEVGTAAMDYRRKTEPPKPMESAEEEPTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJ96</accession>
    <name>R5VJ96_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01349</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000132">
        <property type="protein sequence ID" value="CCZ83581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01349"/>
    <dbReference type="EnsemblBacteria" id="CCZ83581">
        <property type="protein sequence ID" value="CCZ83581"/>
        <property type="gene ID" value="BN566_01349"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="302" mass="34097" checksum="C9BAE4A15740E8F3" modified="2013-07-24" version="1">MTIQQLKYAVTAAEKGTMSEAAQSLFLSQPSLTAAIKELEKELRITIFHRTNKGVIATNEGEEFLGYARQVLQQVGLIEEKYLDGKQLKQIFSVSAQHYSFAVNAFVDVIQTFGNQSYDFTLRETQTFEIIQDVSRLKSEVGVLYLSRENQAVIQKLIAENGLVFEELFTASPHVFISFKHPLAAKTEITLDDLKPYPYLCFEQGDYNAFYFSEEILSTIPREMTIKVRDRATLFNLAVGLDGYTISTGIISKELNGENIIARPLAIPELIRVGTVTRKNMTLSRLGTAYMEALRRHTCNEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJA2</accession>
    <name>R5VJA2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00136</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000135">
        <property type="protein sequence ID" value="CCZ83591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83591">
        <property type="protein sequence ID" value="CCZ83591"/>
        <property type="gene ID" value="BN566_00136"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="63" mass="7323" checksum="E612751325FF5BF3" modified="2013-07-24" version="1">MEQQKLLDELNYHRADKTAKMLLNLGLITTVEYDKLQELNRQKFSPILADLFPKSLDISSKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>R5VKF1</accession>
    <name>R5VKF1_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Isoprenyl transferase</fullName>
            <ecNumber evidence="2">2.5.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01720</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the condensation of isopentenyl diphosphate (IPP) with allylic pyrophosphates generating different type of terpenoids.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="2">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CBAR010000178">
        <property type="protein sequence ID" value="CCZ83991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01720"/>
    <dbReference type="EnsemblBacteria" id="CCZ83991">
        <property type="protein sequence ID" value="CCZ83991"/>
        <property type="gene ID" value="BN566_01720"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00475">
        <property type="entry name" value="Cis_IPPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01139">
        <property type="entry name" value="ISPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001441">
        <property type="entry name" value="UPP_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018520">
        <property type="entry name" value="UPP_synth-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036424">
        <property type="entry name" value="UPP_synth-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291">
        <property type="entry name" value="PTHR10291"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01255">
        <property type="entry name" value="Prenyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64005">
        <property type="entry name" value="SSF64005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00055">
        <property type="entry name" value="uppS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01066">
        <property type="entry name" value="UPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2 3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="31"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="75"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="203"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="30"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="78"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="249" mass="29038" checksum="8F3D3CB23CE2625A" modified="2013-07-24" version="1">MALPFRRKAVSTDTLPVPEQLPKHIGFIMDGNGRWATKRGMPRKFGHREGAKTFHDLTRYCRSIGIQNISFYVFSTENWKRPKEEVDAIMDLLWQYLNDCDEYLEQEVRMIFIGDKSRLPERIQKRMLELEADSAKYHKMNLLLAINYGGRDEIKHATQLIAEEVQAGKLQISDITEDTISQHLYTKEVPDVDLLIRPSGELRLSNFLIWQCAYAEYYFTNVLWPDFSRAELNKALIAYAKRGRRFGGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5VL34</accession>
    <name>R5VL34_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Oxaloacetate decarboxylase alpha subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00185</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000202">
        <property type="protein sequence ID" value="CCZ84256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00185"/>
    <dbReference type="EnsemblBacteria" id="CCZ84256">
        <property type="protein sequence ID" value="CCZ84256"/>
        <property type="gene ID" value="BN566_00185"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003379">
        <property type="entry name" value="Carboxylase_cons_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02436">
        <property type="entry name" value="PYC_OADA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="1">
        <location>
            <begin position="4"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="466" mass="52392" checksum="EE945E9FB15AF773" modified="2013-07-24" version="1">MRKVQLTETVLRDANQSLMATRLPYADYEDILPVMDQTGYYSIECWGGATFDSCLRYLHEDPWERLRKLRKNLPNNKLQMLLRGQNLLGYKHYHDDVVEKFIELSIKNGIDVIRIFDALNDFRNLETALNATMKYATKNTIASGCISYTQSPVHTVDKYIQMCKELKQMGFQTICLKDMAGTMTPYEAEHLIKGIKDAVGDIPLILHTHSTTGMAYMTLTKAIESGIDVIDTATSCFSGGTSQPATETMYYALQQYGIETGLKEDLINQVNDYFKPIKQKYVDNGMINPKSLATDAQALVYKVPGGMLSNMIANLTDMKAMDKFDQALAEIPAVRKDLGYPPLVTPLSQMVGNQAVTNVLLGERYKQISKEVKNYIKGEYGIAPAPVAKELQEKVLGAGGKPVDCRIEDQKRTGVEFQEAAEALGDLARSEEDVMSYICYPDQTLKFLKDRKAQEENEATYTIEEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5VLM3</accession>
    <name>R5VLM3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Prolipoprotein diacylglyceryl transferase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00045</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Lgt family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000230">
        <property type="protein sequence ID" value="CCZ84446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00045"/>
    <dbReference type="EnsemblBacteria" id="CCZ84446">
        <property type="protein sequence ID" value="CCZ84446"/>
        <property type="gene ID" value="BN566_00045"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008961">
        <property type="term" value="F:phosphatidylglycerol-prolipoprotein diacylglyceryl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042158">
        <property type="term" value="P:lipoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001640">
        <property type="entry name" value="Lgt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30589">
        <property type="entry name" value="PTHR30589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01790">
        <property type="entry name" value="LGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="43"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="85"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="200"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="237"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="263" mass="28993" checksum="583688C14B5733C7" modified="2013-07-24" version="1">MYPILSLFGFSFGTYGICCVIGGALAIWLAIRNGKRFQQDRFAMLEACVWALIGGFLGAKLLYLLVSFSDIVEMFQTSGFNWNTISSLLEGGFVFYGGLLGGLAALLLYLHHFQLPYGVYLSSITPAVPLAHAFGRIGCFFAGCCYGIPYDPPIGICLKNAIGTAPTDIPLFPVQLLESGLDFLLAGLLQLCFRKRTRKTDVIPFYCIGYAGIRLFTERFRYDTERGIYFGLATSEWISLALAICGVLLLLVPSLRRKRASHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>R5VLP8</accession>
    <name>R5VLP8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Formate--tetrahydrofolate ligase</fullName>
            <ecNumber evidence="6">6.3.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Formyltetrahydrofolate synthetase</fullName>
            <shortName evidence="6">FHS</shortName>
            <shortName evidence="6">FTHFS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">fhs</name>
        <name evidence="7" type="ORF">BN566_00365</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-formyltetrahydrofolate + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20221"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57454"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the formate--tetrahydrofolate ligase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.3" evidence="6"/>
    <dbReference type="EMBL" id="CBAR010000238">
        <property type="protein sequence ID" value="CCZ84466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00365"/>
    <dbReference type="EnsemblBacteria" id="CCZ84466">
        <property type="protein sequence ID" value="CCZ84466"/>
        <property type="gene ID" value="BN566_00365"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004329">
        <property type="term" value="F:formate-tetrahydrofolate ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00477">
        <property type="entry name" value="FTHFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01543">
        <property type="entry name" value="FTHFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000559">
        <property type="entry name" value="Formate_THF_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020628">
        <property type="entry name" value="Formate_THF_ligase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01268">
        <property type="entry name" value="FTHFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00721">
        <property type="entry name" value="FTHFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00722">
        <property type="entry name" value="FTHFS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 6" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="65"/>
            <end position="72"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ84466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="556" mass="59653" checksum="5E5A866A9A1A5911" modified="2013-07-24" version="1">MLSDIEIAQQAEMQKIREVAAGLSIEEDDLEYYGHYKAKLSESLFQKLEDKPNGKLILVTAINPTPAGEGKTTVSVGLADALRCIGKKSVVALREPSLGPVFGIKGGAAGGGYSQVVPMEDINLHFTGDMHAITAANNLLCAMLDNHMQQGNVLRIDQRRVMFKRVLDMNDRALRNIVIGLGGKVDGIPRSDSFQITVASEVMAILCLASDLADMKRRLGQILVAYDLDGNPVYAKDLGAEGAMTALLKDALKPNLVQTLAHTPALIHGGPFANIAHGCNSLRATKLALKLGDYCVTEAGFGSDLGAEKFFDIKCRYGGLKPDCVVLVATVRALKYNGGVPKTELTTENLDALKKGIVNLQVHIENMQKYGVPVVVAINRFHTDTEAELQFVKEFCASLGASCELAEVFAKGGEGGKALAETVCKTIEEKPSQFHVLYDTEQTIEEKVNCIAKEIYRADGVIFTAPAKKALAQIVALGYDKLPICVAKTQYSLSDDASQLGHPTDFKITVRDVTLSAGAGFVVVLTGNIMTMPGLPKAPAALRIDCDNNGNITGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>R5VM06</accession>
    <name>R5VM06_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit C</fullName>
            <shortName evidence="6">Asp/Glu-ADT subunit C</shortName>
            <ecNumber evidence="6">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">gatC</name>
        <name evidence="7" type="ORF">BN566_00412</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>ATP + H2O + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + 2 H(+) + L-asparaginyl-tRNA(Asn) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14513"/>
            <dbReference type="Rhea" id="RHEA-COMP:9674"/>
            <dbReference type="Rhea" id="RHEA-COMP:9677"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78515"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 6">
            <text>ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) + L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4 6">Heterotrimer of A, B and C subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the GatC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="6"/>
    <dbReference type="EMBL" id="CBAR010000243">
        <property type="protein sequence ID" value="CCZ84551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00412"/>
    <dbReference type="EnsemblBacteria" id="CCZ84551">
        <property type="protein sequence ID" value="CCZ84551"/>
        <property type="gene ID" value="BN566_00412"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050566">
        <property type="term" value="F:asparaginyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00122">
        <property type="entry name" value="GatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003837">
        <property type="entry name" value="Asp/Glu-ADT_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036113">
        <property type="entry name" value="Asp/Glu-ADT_sf_sub_c"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02686">
        <property type="entry name" value="Glu-tRNAGln"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141000">
        <property type="entry name" value="SSF141000"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00135">
        <property type="entry name" value="gatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ84551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="89" mass="10096" checksum="6DE2261706973A53" modified="2013-07-24" version="1">MKIDIAHIAKLSRLHFEPEQIAKFEKDMESIVAMVDHLPDVSDTLTVDPNNAMRLREDVAKDHKFKREEMLANAPEVQAGCLVVPKTVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>R5VM66</accession>
    <name>R5VM66_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit biogenesis GTPase RsgA</fullName>
            <ecNumber evidence="7">3.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rsgA</name>
        <name evidence="10" type="ORF">BN566_00489</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">One of several proteins that assist in the late maturation steps of the functional core of the 30S ribosomal subunit. Helps release RbfA from mature subunits. May play a role in the assembly of ribosomal proteins into the subunit. Circularly permuted GTPase that catalyzes slow GTP hydrolysis, GTPase activity is stimulated by the 30S ribosomal subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Monomer. Associates with 30S ribosomal subunit, binds 16S rRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TRAFAC class YlqF/YawG GTPase family. RsgA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.-" evidence="7"/>
    <dbReference type="EMBL" id="CBAR010000257">
        <property type="protein sequence ID" value="CCZ84636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00489"/>
    <dbReference type="EnsemblBacteria" id="CCZ84636">
        <property type="protein sequence ID" value="CCZ84636"/>
        <property type="gene ID" value="BN566_00489"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04466">
        <property type="entry name" value="S1_YloQ_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01854">
        <property type="entry name" value="YjeQ_EngC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01820">
        <property type="entry name" value="GTPase_RsgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030378">
        <property type="entry name" value="G_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004881">
        <property type="entry name" value="Ribosome_biogen_GTPase_RsgA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010914">
        <property type="entry name" value="RsgA_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031944">
        <property type="entry name" value="RsgA_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32120">
        <property type="entry name" value="PTHR32120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03193">
        <property type="entry name" value="RsgA_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16745">
        <property type="entry name" value="RsgA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00157">
        <property type="entry name" value="TIGR00157"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50936">
        <property type="entry name" value="ENGC_GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51721">
        <property type="entry name" value="G_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CP-type G" evidence="9">
        <location>
            <begin position="62"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="ENGC_GTPASE" evidence="8">
        <location>
            <begin position="71"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="111"/>
            <end position="114"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="159"/>
            <end position="167"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ84636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="290" mass="31922" checksum="FF9D5BA85F6348D5" modified="2013-07-24" version="1">MMQEKESGRILQCIGGIYTVATDSGTVSCKARGVFRKRGMTPYAGDFVTVEQGVMTEILPRKNSIIRPPLANLDQLLFVTSTYKPVPNLLLLDQFMAVAIYKQIQPVLVLTKLDLQDGAALQKLYTNAGIPMYPVCYDQPDTLEAVRACLRGKVSALTGNSGAGKSTLLNALYPDLQLKVGEISEKLGRGRHTTRQASLYPLDGGYIADTPGFSTFEQEQYARIPKEELADCFPEFAPYQGSCRFQDCAHVCEKGCGVLAAVQDGSIAESRHASYVAMYEIAKKRKDWEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5VQC7</accession>
    <name>R5VQC7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized NAD-dependent dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00592</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000008">
        <property type="protein sequence ID" value="CCZ82787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00592"/>
    <dbReference type="EnsemblBacteria" id="CCZ82787">
        <property type="protein sequence ID" value="CCZ82787"/>
        <property type="gene ID" value="BN566_00592"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042204">
        <property type="entry name" value="2Fe-2S-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="SSF54292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="104" mass="11518" checksum="3A0B7457D41867F1" modified="2013-07-24" version="1">MRISEHPIIESYNKGRKVTFSFDGKELSGYEGESIAAALKAAGVMVHRYTAKQHKPRGIFCAIGRCTDCVMIVNGVPNVRTCVTPLEEGMQIQTQYGVEPKKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5VQD4</accession>
    <name>R5VQD4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00602</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000008">
        <property type="protein sequence ID" value="CCZ82797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00602"/>
    <dbReference type="EnsemblBacteria" id="CCZ82797">
        <property type="protein sequence ID" value="CCZ82797"/>
        <property type="gene ID" value="BN566_00602"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046088">
        <property type="entry name" value="DUF6106"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19601">
        <property type="entry name" value="DUF6106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="171" mass="19071" checksum="5C2705820D63E5AB" modified="2013-07-24" version="1">MDHFAEYLVQKRPTSQDRLKQGLIVAAAVVLSVLIGAISVLTGWLFLLVLVIGAIYGAYYLLTGCYTEYEYTVTNGELDVDKIAGRRKRSHLLTVPVSKFTGFGVYTDQTDDPSDATLFLCSDNTGENAYYADFTTEEYGTARLIFTPNEEMVQTIVLFLPAALKRQFRQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>R5VQE2</accession>
    <name>R5VQE2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Mannan endo-1 4-beta-mannosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN566_00612</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000008">
        <property type="protein sequence ID" value="CCZ82807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00612"/>
    <dbReference type="EnsemblBacteria" id="CCZ82807">
        <property type="protein sequence ID" value="CCZ82807"/>
        <property type="gene ID" value="BN566_00612"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008810">
        <property type="term" value="F:cellulase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005102">
        <property type="entry name" value="Carbo-bd_X2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005087">
        <property type="entry name" value="CBM_fam11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002105">
        <property type="entry name" value="Dockerin_1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045053">
        <property type="entry name" value="MAN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31451">
        <property type="entry name" value="PTHR31451"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03425">
        <property type="entry name" value="CBM_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03442">
        <property type="entry name" value="CBM_X2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00404">
        <property type="entry name" value="Dockerin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="SSF81296"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00448">
        <property type="entry name" value="CLOS_CELLULOSOME_RPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004407254" evidence="7">
        <location>
            <begin position="31"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="8">
        <location>
            <begin position="841"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="796"/>
            <end position="841"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ82807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="909" mass="99923" checksum="73EC7B72E6CD6342" modified="2013-07-24" version="1">MKKWIALATGLSLVLSGFAAAGFQPSKVQAADRTGFVQTDGTQFLLDGSTFYYAGTNNYYLNFKPQYEVDQVMEDAAAMGLKVIRTWGNLDAGVKTDKVNKDGYTVFTDSVDGSGEKEGVYYQYFDADLGRPVVNEGKDGLQKLDYAIYKAEQEGVRLLITFTNNWEAFGGMNQYVKWAKLAGENVTGHDDFYTNETIKGWYKDYIKTLLNHENVYTGVKYKDDPTIFSWELANEPRCESDAGCENHVVENWASEMSDYVKSIDSNHMLAVGDEGFYNYGYNDFPEGDHKYVYHGSSGMDWLSLTNLPNIDYGTIHVYCDQWGLTKEQGNFWFKKHGEDAAAMNKPVIVEEFGWKDKSERADVYNEWFDIFEGNTYEGVEYAGTNYWMLASLTGDGTLYQDYDGYTVYYKGDANGNPTQDACDAIMAHAQRMDAKNTQNSVSPKKANFDIVKPADIQLRTTMELGSVSGVQFGDKMLTEGTDYTVEDNVITISAKVLEGLELGNYKITILTTDGNQPTVLLSVIDSNAETQKKSVIDNFESYADDAELAAAYHRNSSGDSLTVSLDAEHAKNGSYAMKYEYSVADGGAGYCGTTKKLNGADWTGFDGIHFWILSDGSNRDTTIQFIDGAGAYWESIQHVTAETGWQEVKIPFSDFRLQQWGTAAETPTLNGVSEYSIYTGQNGNPGTGVWYFDDIGLYQDGATVPDAEVVESSATFSKQAPADVIFTIITNGHAVTGISEEGEALQQGVDFAINGSQFRFNQSYLETLSEGVHTYHVGFATCPDLVLTITVTGSTVTTTETTTEPSETTTTTTTTETTSTETEVTTGSESETTTTTTDADTDTNYGDVNLDGKVDLVDAITINKYLAGQITLSEQATKNADVNADGSLGDGDSTTLMQFVLMMIPNLPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>R5VRB2</accession>
    <name>R5VRB2_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ion-translocating oxidoreductase complex subunit C</fullName>
            <ecNumber evidence="8">7.-.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Rnf electron transport complex subunit C</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rnfC</name>
        <name evidence="11" type="ORF">BN566_00880</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Part of a membrane-bound complex that couples electron transfer with translocation of ions across the membrane.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="8">Binds 2 [4Fe-4S] clusters per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">The complex is composed of six subunits: RnfA, RnfB, RnfC, RnfD, RnfE and RnfG.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the 4Fe4S bacterial-type ferredoxin family. RnfC subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.-.-.-" evidence="8"/>
    <dbReference type="EMBL" id="CBAR010000054">
        <property type="protein sequence ID" value="CCZ83092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00880"/>
    <dbReference type="EnsemblBacteria" id="CCZ83092">
        <property type="protein sequence ID" value="CCZ83092"/>
        <property type="gene ID" value="BN566_00880"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00461">
        <property type="entry name" value="RsxC_RnfC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010208">
        <property type="entry name" value="Ion_transpt_RnfC/RsxC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011538">
        <property type="entry name" value="Nuo51_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037225">
        <property type="entry name" value="Nuo51_FMN-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026902">
        <property type="entry name" value="RnfC_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43034">
        <property type="entry name" value="PTHR43034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01512">
        <property type="entry name" value="Complex1_51K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13237">
        <property type="entry name" value="Fer4_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13375">
        <property type="entry name" value="RnfC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142019">
        <property type="entry name" value="SSF142019"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01945">
        <property type="entry name" value="rnfC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6 8" id="KW-0408">Iron</keyword>
    <keyword evidence="7 8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-1278">Translocase</keyword>
    <keyword evidence="1 8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="10">
        <location>
            <begin position="348"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="10">
        <location>
            <begin position="387"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="357"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="360"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="363"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="367"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="396"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="399"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="402"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="406"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ83092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="429" mass="46259" checksum="69B3BCACAFD9740F" modified="2013-07-24" version="1">MRKKLSGVKLAHHKNTANTASQPLPLPKRVRIPMSMHIGAPCSPLVKVRDSICVGQKIGDTDAFMAAPIHSGVSGTVKAVSDFRMSNGRTCKCVEIETDGLQTVADTVQPPVISSKEDFLHAIRESGLVGMGGASFPTHVKLAPKQTVDTLCINAAECEPYITSDNRQMVEHPEEVIDGIQQVLHWLNIPHCIIGVESNKPEAIQILTEKAASDNRIQVVTLPSTYPQGAEKVLIYHTLGRIVAEGELPADQGVIVMNVSSVAFVSRYLKTGMPMVQRMVTVDGDCIGNPCNVIVPMGTPVEDVLTFAKCDLERAKKLLYGGPMMGMAIADLQDPILKANNAIVAFSKLEETAPSACIHCGRCIHACPLRLMPPQIAKAYRQKDVAALQALHVTLCMNCGCCTYACPAKRPVAETNQFAKAMAMAAMKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>R5VRF1</accession>
    <name>R5VRF1_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 13 14">UvrABC system protein B</fullName>
            <shortName evidence="13">Protein UvrB</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Excinuclease ABC subunit B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">uvrB</name>
        <name evidence="18" type="ORF">BN566_00924</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 13">The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA(2)B(2) complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11 13 14">Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="13">The beta-hairpin motif is involved in DNA binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13 14">Belongs to the UvrB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000062">
        <property type="protein sequence ID" value="CCZ83137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00924"/>
    <dbReference type="EnsemblBacteria" id="CCZ83137">
        <property type="protein sequence ID" value="CCZ83137"/>
        <property type="gene ID" value="BN566_00924"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009380">
        <property type="term" value="C:excinuclease repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009381">
        <property type="term" value="F:excinuclease ABC activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00204">
        <property type="entry name" value="UvrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001943">
        <property type="entry name" value="UVR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036876">
        <property type="entry name" value="UVR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004807">
        <property type="entry name" value="UvrB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041471">
        <property type="entry name" value="UvrB_inter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024759">
        <property type="entry name" value="UvrB_YAD/RRR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24029">
        <property type="entry name" value="PTHR24029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12344">
        <property type="entry name" value="UvrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17757">
        <property type="entry name" value="UvrB_inter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46600">
        <property type="entry name" value="SSF46600"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00631">
        <property type="entry name" value="uvrb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 13 14" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6 13 14" id="KW-0228">DNA excision</keyword>
    <keyword evidence="9 13 14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8 13 14" id="KW-0267">Excision nuclease</keyword>
    <keyword evidence="4 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 14" id="KW-0742">SOS response</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="16">
        <location>
            <begin position="25"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="17">
        <location>
            <begin position="429"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="domain" description="UVR" evidence="15">
        <location>
            <begin position="618"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Beta-hairpin" evidence="13">
        <location>
            <begin position="91"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00026033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CCZ83137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="655" mass="74249" checksum="E92D7C0662FC75E1" modified="2013-07-24" version="1">MSEFRLHAPYQPTGDQPEAIAELVNGIRAGDHEQTLLGVTGSGKTFTIANVIAQVNKPTLVLAHNKILAAQLCSEFKEFFPENAVEYFVSYYDYYQPEAYVPSTDSYIEKDSSINDEIDKLRHSATTALAERRDVIIVASVSCIYSLGSPEEYRSMVVSVRKGMEKPREQLLKELVAIQYERNDVQFVRNKFRVRGDVVEIFPSNSNHTAIRVEYFGDEIDRICEVDTVTGNVTATLQHAAIFPASHYVVSGEHVEQAISEIETELQERVAYFEANHRLIEAQRIAQRTHYDMEMLREIGFCSGIENYSRILAGRPAGSVPYTLLDHFPNDFLLVVDESHVTLPQVRAMSAGDHARKATLVDYGFRLPSAFDNRPLNFDEFYERIPQAIYVSATPGEFEKQHSSAIVEQVIRPTGLLDPMISVRPVSGQIEDLYSEIQKRVERNERVLITTLTKKMAEDLTAYLEKLSVRVQYLHHDVDTVKRMEIIRDLRLGNFDVLVGINLLREGLDIPEVSLVAILDADKEGFLRSETALIQTIGRAARNANGMVILYADEVTGSMERAITETERRRKKQMAYNTAHHITPKTITKAVRDVIEISTKEEVEEQTAAPKMTEQERKALMETLTAQMKEAAKLLEFEHAAYLRDKIQELRGKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>R5VRY6</accession>
    <name>R5VRY6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Amino acid ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_01103</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000084">
        <property type="protein sequence ID" value="CCZ83322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01103"/>
    <dbReference type="EnsemblBacteria" id="CCZ83322">
        <property type="protein sequence ID" value="CCZ83322"/>
        <property type="gene ID" value="BN566_01103"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010065">
        <property type="entry name" value="AA_ABC_transptr_permease_3TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043429">
        <property type="entry name" value="ArtM/GltK/GlnP/TcyL/YhdX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30614">
        <property type="entry name" value="PTHR30614"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01726">
        <property type="entry name" value="HEQRo_perm_3TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="62"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="85"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="185"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="7">
        <location>
            <begin position="14"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="217" mass="24837" checksum="EFBFDCE76BCB01B3" modified="2013-07-24" version="1">MSFFEIIAQLGDSMLRTLAIFLLTLVFSIPLGMIVCVMRMYGWKPLQWLARIYISVLRGTPLMLQLLVVYFGPYFIFQIPLSTSYRFYAVIIAFSLNYAAYFAEIFRSGIQSIPVGQYEASRILGYNRVQMFLRIILPQVIKRILPAVTNETITLVKDTSLAFAIAYTELFTEVKQIAAAQTSMLPYLAAAIFYYVFNLLVAGLMALVEKKMNYYKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VS39</accession>
    <name>R5VS39_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01138</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000093">
        <property type="protein sequence ID" value="CCZ83362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83362">
        <property type="protein sequence ID" value="CCZ83362"/>
        <property type="gene ID" value="BN566_01138"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="76" mass="8811" checksum="166977C8366BB989" modified="2013-07-24" version="1">MLSMSELAMNPNRKVTTKCYGEVKVWNDREEAQAYFLEAMMNSDGAEHDRYSGIYIQLQNGLDYCTDEDDDEEDES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>R5VSP9</accession>
    <name>R5VSP9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="5">PPIase</shortName>
            <ecNumber evidence="5">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_01320</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 5">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="5"/>
    <dbReference type="EMBL" id="CBAR010000130">
        <property type="protein sequence ID" value="CCZ83552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01320"/>
    <dbReference type="EnsemblBacteria" id="CCZ83552">
        <property type="protein sequence ID" value="CCZ83552"/>
        <property type="gene ID" value="BN566_01320"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000413">
        <property type="term" value="P:protein peptidyl-prolyl isomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024936">
        <property type="entry name" value="Cyclophilin-type_PPIase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044666">
        <property type="entry name" value="Cyclophilin_A-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625">
        <property type="entry name" value="PTHR45625"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001467">
        <property type="entry name" value="Peptidylpro_ismrse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="SSF50891"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="6">
        <location>
            <begin position="1"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ83552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="157" mass="17079" checksum="22EFB17965D4C2B2" modified="2013-07-24" version="1">MVVIEMENGKKIELELYPEIAPITCENFEKLVKQGFYNGLIFHRVIPGFMIQGGCPDGTGMGGPGWNIKGEFAANGVKNDLKHTRGVISMARAMNPNSAGSQFFIMHEDAPHLDGQYAAFGKVVSGMDVVDEIASVQTDYADKPLTPQVMKSVTIVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5VSS9</accession>
    <name>R5VSS9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_01355</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000134">
        <property type="protein sequence ID" value="CCZ83587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01355"/>
    <dbReference type="EnsemblBacteria" id="CCZ83587">
        <property type="protein sequence ID" value="CCZ83587"/>
        <property type="gene ID" value="BN566_01355"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000292">
        <property type="entry name" value="For/NO2_transpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30520">
        <property type="entry name" value="PTHR30520"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01226">
        <property type="entry name" value="Form_Nir_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="72"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="130"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ83587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="228" mass="24845" checksum="4119E2CDAC7E692A" modified="2013-07-24" version="1">MEQTKPNKNKWISFLADGILSGLMLGIGGMVSLSSENRFLGAFLFSLGLFCIVQFKYGLFTGKVGYIVSRDLPYVAETAVTLVGNALGTLIAALLLRQVRFFTSTVSTMDVTISDRVQATVDGKIHDNPLSIFILAIFCGILMFTAVEGSRKCKEEKNFAGSLFVVVMPVMVFIICGFNHCIADMFYYFFAGCPEPSKAIVYFILAIAGNSVGGMIVPLLKKLSNQPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VSZ8</accession>
    <name>R5VSZ8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01398</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000148">
        <property type="protein sequence ID" value="CCZ83662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83662">
        <property type="protein sequence ID" value="CCZ83662"/>
        <property type="gene ID" value="BN566_01398"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="236" mass="27558" checksum="54F2A55B63DCC2DB" modified="2013-07-24" version="1">MLESGYIKLYRSLLNWEWYDDINTKTVFLHLLLTVNIAKRQWHGITVPCGSRVCSYAVLASETRLSVDKVRTAIKHLEATGEITRCKYPKCTVFTVNNYDKFQNVPSISQGDYQDSPEVVPKSSQQNKKIEEDKEDIYLSILDAESQNFPPTLEEIRLFAEQEKIRIDVQKFYDYYTERDWKTKNGNFIRNWKKTLQYWGETEGTPHKGKKQQQETPVSENAEAYASLILNLDEPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>R5VT64</accession>
    <name>R5VT64_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator AraC family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01462</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000158">
        <property type="protein sequence ID" value="CCZ83727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01462"/>
    <dbReference type="EnsemblBacteria" id="CCZ83727">
        <property type="protein sequence ID" value="CCZ83727"/>
        <property type="gene ID" value="BN566_01462"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003313">
        <property type="entry name" value="AraC-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037923">
        <property type="entry name" value="HTH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018062">
        <property type="entry name" value="HTH_AraC-typ_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020449">
        <property type="entry name" value="Tscrpt_reg_HTH_AraC-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02311">
        <property type="entry name" value="AraC_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00032">
        <property type="entry name" value="HTHARAC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51215">
        <property type="entry name" value="SSF51215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00041">
        <property type="entry name" value="HTH_ARAC_FAMILY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="2">
        <location>
            <begin position="180"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="297" mass="34238" checksum="444B9387050C4471" modified="2013-07-24" version="1">MKDLIFSVFPSENFVDLGLYQFGWEKCKPAHSFGPAARNHYLFHYIMSGTGRLMTTDENGQTQSFSIKSRQGFMIFPNQITTYIADKDLPWEYVWVEFDGLRVKSILDACGFSPNHPVYHAKSKDLREEMMNEMIYITQNQNSSPIHLIGHLYLFLDYLARSTASASLSSGSSLRDFYIHEALNYIEHNFQNDITVEDIADVCGLNRSYFGKIFKNAVGKTPQEFLLSYRMLKATELLKLTRLSIRDVSNAVGYANPLHFSRAFRNIYGIPPRDWRNQNQIFLPDPLSDWSDSGYDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5VT92</accession>
    <name>R5VT92_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01497</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000161">
        <property type="protein sequence ID" value="CCZ83762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01497"/>
    <dbReference type="EnsemblBacteria" id="CCZ83762">
        <property type="protein sequence ID" value="CCZ83762"/>
        <property type="gene ID" value="BN566_01497"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001890">
        <property type="entry name" value="RNA-binding_CRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017924">
        <property type="entry name" value="RNA-binding_YhbY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035920">
        <property type="entry name" value="YhbY-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01985">
        <property type="entry name" value="CRS1_YhbY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01103">
        <property type="entry name" value="CRS1_YhbY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75471">
        <property type="entry name" value="SSF75471"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00253">
        <property type="entry name" value="RNA_bind_YhbY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51295">
        <property type="entry name" value="CRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="CRM" evidence="3">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="96" mass="10704" checksum="5A90CCE6DC7D2313" modified="2013-07-24" version="1">MLTSKQRATLRGIASSYETILQVGKNGIGETLIQQVSDALRKRELIKLHVLENSELSAREAAEQLAEATESEVVQVIGSRFVLYKRNPKKPVIDLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5VU70</accession>
    <name>R5VU70_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Penicillin V acylase and related amidases</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01783</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C59 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000184">
        <property type="protein sequence ID" value="CCZ84057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01783"/>
    <dbReference type="EnsemblBacteria" id="CCZ84057">
        <property type="protein sequence ID" value="CCZ84057"/>
        <property type="gene ID" value="BN566_01783"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029132">
        <property type="entry name" value="CBAH/NAAA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02275">
        <property type="entry name" value="CBAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBAH" evidence="3">
        <location>
            <begin position="2"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="324" mass="36652" checksum="AF9EBEF835000F2B" modified="2013-07-24" version="1">MCTAVTYQTNHFYFGRTLDYESSYGEEIVILPRKFPLSFLHRGTVTQHYAIMGIAHVANQYPLFYDAINEKGLCMAGLNFVGNAHYVKSTTEENEVAQYEFIPWILSTCATTAEAKERIASICITDTPFCEQMPVGQLHWMIADKNQTITVEAVADGIKIYENLVGVLTNNPPFPEQLFRLNDFMHLSPKQPQNHFSSTLSLQPYSRGMGALGLPGDLSSQSRFIRAAFVRANSVSGTSEQENVSQFFHILGAVEQQRGCCDVGNGQYEITIYTDCYNAEKGIFYYTTYQNHQISAVHLHNEPLDSDFLIRYPMITGEQIHYQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5VU96</accession>
    <name>R5VU96_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Acyl-coenzyme A synthetases/AMP-(Fatty) acid ligases</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01813</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000186">
        <property type="protein sequence ID" value="CCZ84087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01813"/>
    <dbReference type="EnsemblBacteria" id="CCZ84087">
        <property type="protein sequence ID" value="CCZ84087"/>
        <property type="gene ID" value="BN566_01813"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-binding" evidence="1">
        <location>
            <begin position="38"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding_C" evidence="2">
        <location>
            <begin position="463"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="556" mass="62924" checksum="AE7A8A1C2823E76D" modified="2013-07-24" version="1">MLLDKYLPRIEFDSYEDFKQNYRVNVPETFNFGFDIVDEWAKQEPEKKALVWCDDDGNERTFTFTEISRLSNQTANYFQHLGIRKGTVVMLILRRRWEYWVCAVALHKIGAILIPGSLQLSKKDLVYRGNSAGIHTMICVDDAYVVEQVEAAQSEVPSIQHRVLVAGKRDGWQDFEEEIAKYSDVFPRPTGAAATKANETAIVYFTSGTTGDPKMVQHNHAHPLGHIVTAKYWQQVQENKLHMSVSDSGWAKFGWGKIYGQWICGAVIFAYDMKKFIPTHLLQKMQDYKLTTFCAPPTMYRFMLQEDVKAYDLSSIETFATAGEPLNPEVFNKWKELTGKEIREGFGQTEGSVLIANFPWLTPKPGSTGKPSPLYDIHLITDEGVDAKAGEHGALTMLHLKESYPIGLFTGYYHNPEMTNEILGDGTYCSHDVFEPDEDGYFWFVGRNDDVIKCSGYRIGPFEVESALVAHPAVVECAVTAAPDPIRGNVVKATVVLAKGYEPSEALTKELQNHVKHTTAPYKYPRIIEYVEELPKTLGGKIKRAQIRKEDFAKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5VUQ4</accession>
    <name>R5VUQ4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RES domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00181</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000198">
        <property type="protein sequence ID" value="CCZ84252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84252">
        <property type="protein sequence ID" value="CCZ84252"/>
        <property type="gene ID" value="BN566_00181"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014914">
        <property type="entry name" value="RES_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08808">
        <property type="entry name" value="RES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RES" evidence="1">
        <location>
            <begin position="140"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="370" mass="43054" checksum="816093B88C783C38" modified="2013-07-24" version="1">MESNWLQNEFIWKELRAPYIINYDTDYYETLKDKYEILLNQAIKAKADEESIEIIKNYKKKILEALNAYYSADLSESSTIIRNLIEDIGDDPFAVNTLQESAAFGGSGGEEIQFFRCRTGNPSSSFTSKEMLHLPKEMRAKSGNYRFSIPGNPSLYLSNSSYGCWIETGFPSEINFNVSPVILDGTQKIFNLAVSSRDFFITHDLDLDEIHCWLKLYMLSIATSFRINEKERTFKSEYIISQAIMMACKKIGYDGIAYYSKRVSDEIFSLCAINLVLFVDYDNEYSKITKHIKIDDPFNYALYKQLFPSSHYKNYNLRSVQTGLVTNIGSFDRQYPYRETEFFEFDKFLFTTWRDKPKKGKDQIPWGVDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>R5VV45</accession>
    <name>R5VV45_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">D-aminoacyl-tRNA deacylase</fullName>
            <shortName evidence="1">DTD</shortName>
            <ecNumber evidence="1">3.1.1.96</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Gly-tRNA(Ala) deacylase</fullName>
            <ecNumber evidence="1">3.1.1.-</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dtd</name>
        <name evidence="2" type="ORF">BN566_00294</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">An aminoacyl-tRNA editing enzyme that deacylates mischarged D-aminoacyl-tRNAs. Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS. Acts via tRNA-based rather than protein-based catalysis; rejects L-amino acids rather than detecting D-amino acids in the active site. By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo and helps enforce protein L-homochirality.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a D-aminoacyl-tRNA + H2O = a D-alpha-amino acid + a tRNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13953"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:10124"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59871"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:79333"/>
            <dbReference type="EC" id="3.1.1.96"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>glycyl-tRNA(Ala) + H2O = glycine + H(+) + tRNA(Ala)</text>
            <dbReference type="Rhea" id="RHEA:53744"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:13640"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78522"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">A Gly-cisPro motif from one monomer fits into the active site of the other monomer to allow specific chiral rejection of L-amino acids.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DTD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.96" evidence="1"/>
    <dbReference type="EC" id="3.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="CBAR010000222">
        <property type="protein sequence ID" value="CCZ84387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00294"/>
    <dbReference type="EnsemblBacteria" id="CCZ84387">
        <property type="protein sequence ID" value="CCZ84387"/>
        <property type="gene ID" value="BN566_00294"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051499">
        <property type="term" value="F:D-aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106026">
        <property type="term" value="F:Gly-tRNA(Ala) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043908">
        <property type="term" value="F:Ser(Gly)-tRNA(Ala) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019478">
        <property type="term" value="P:D-amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00563">
        <property type="entry name" value="Dtyr_deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00518">
        <property type="entry name" value="Deacylase_Dtd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003732">
        <property type="entry name" value="Daa-tRNA_deacyls_DTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023509">
        <property type="entry name" value="DTD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10472">
        <property type="entry name" value="PTHR10472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02580">
        <property type="entry name" value="Tyr_Deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69500">
        <property type="entry name" value="SSF69500"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00256">
        <property type="entry name" value="TIGR00256"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0820">tRNA-binding</keyword>
    <feature type="short sequence motif" description="Gly-cisPro motif, important for rejection of L-amino acids" evidence="1">
        <location>
            <begin position="137"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="150" mass="16363" checksum="3B970D95414E3C81" modified="2013-07-24" version="1">MRLIIQRVTQASVTVSGSVVGAINKGYLVLVGIGQNDTEAIVEKMADKMMRLRIFSDENDKINLSLQDVGGQLLLVSQFTLYADCRKGNRPSFIAAGAPKTAEALYEYMIAYCKQTVPVVEHGIFGADMQVSLCNDGPFTIVLDSDEIVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>R5VV82</accession>
    <name>R5VV82_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">ATP synthase subunit c</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">ATP synthase F(0) sector subunit c</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">F-type ATPase subunit c</fullName>
            <shortName evidence="13">F-ATPase subunit c</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Lipid-binding protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">atpE</name>
        <name evidence="15" type="ORF">BN566_00324</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 13">F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation.</text>
    </comment>
    <comment type="function">
        <text evidence="13">Key component of the F(0) channel; it plays a direct role in translocation across the membrane. A homomeric c-ring of between 10-14 subunits forms the central stalk rotor element with the F(1) delta and epsilon subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cell membrane</location>
            <topology evidence="13">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the ATPase C chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000225">
        <property type="protein sequence ID" value="CCZ84417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00324"/>
    <dbReference type="EnsemblBacteria" id="CCZ84417">
        <property type="protein sequence ID" value="CCZ84417"/>
        <property type="gene ID" value="BN566_00324"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045263">
        <property type="term" value="C:proton-transporting ATP synthase complex, coupling factor F(o)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01396">
        <property type="entry name" value="ATP_synth_c_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005953">
        <property type="entry name" value="ATP_synth_csu_bac/chlpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000454">
        <property type="entry name" value="ATP_synth_F0_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020537">
        <property type="entry name" value="ATP_synth_F0_csu_DDCD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038662">
        <property type="entry name" value="ATP_synth_F0_csu_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035921">
        <property type="entry name" value="F/V-ATP_Csub_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10031">
        <property type="entry name" value="PTHR10031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00124">
        <property type="entry name" value="ATPASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81333">
        <property type="entry name" value="SSF81333"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01260">
        <property type="entry name" value="ATP_synt_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00605">
        <property type="entry name" value="ATPASE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 13" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 13" id="KW-0138">CF(0)</keyword>
    <keyword evidence="6 13" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="8 13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 13" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="43"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-synt_C" evidence="14">
        <location>
            <begin position="2"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="site" description="Reversibly protonated during proton transport" evidence="13">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CCZ84417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="68" mass="6666" checksum="4337051C1804F3A6" modified="2013-07-24" version="1">MIAAGIAVLTGLGAGLGIGIATSKATEAIARQPEAAGDINKTLLLGSALAEATAIYGFVVALLLVIMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVA0</accession>
    <name>R5VVA0_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Methionine synthase</fullName>
            <ecNumber evidence="6">2.1.1.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">5-methyltetrahydrofolate--homocysteine methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">BN566_00339</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Catalyzes the transfer of a methyl group from methyl-cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6S)-5-methyl-5,6,7,8-tetrahydrofolate + L-homocysteine = (6S)-5,6,7,8-tetrahydrofolate + L-methionine</text>
            <dbReference type="Rhea" id="RHEA:11172"/>
            <dbReference type="ChEBI" id="CHEBI:18608"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
            <dbReference type="EC" id="2.1.1.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 19">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>methylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:28115"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetH route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the vitamin-B12 dependent methionine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.13" evidence="6"/>
    <dbReference type="EMBL" id="CBAR010000225">
        <property type="protein sequence ID" value="CCZ84432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00339"/>
    <dbReference type="EnsemblBacteria" id="CCZ84432">
        <property type="protein sequence ID" value="CCZ84432"/>
        <property type="gene ID" value="BN566_00339"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00081"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008705">
        <property type="term" value="F:methionine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042558">
        <property type="term" value="P:pteridine-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1240.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003759">
        <property type="entry name" value="Cbl-bd_cap"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006158">
        <property type="entry name" value="Cobalamin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036724">
        <property type="entry name" value="Cobalamin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003726">
        <property type="entry name" value="HCY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036589">
        <property type="entry name" value="HCY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017215">
        <property type="entry name" value="MetH_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036594">
        <property type="entry name" value="Meth_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000489">
        <property type="entry name" value="Pterin-binding_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02310">
        <property type="entry name" value="B12-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02607">
        <property type="entry name" value="B12-binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00809">
        <property type="entry name" value="Pterin_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02574">
        <property type="entry name" value="S-methyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037472">
        <property type="entry name" value="DHPS_mtfrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01018">
        <property type="entry name" value="B12-binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47644">
        <property type="entry name" value="SSF47644"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="SSF51717"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52242">
        <property type="entry name" value="SSF52242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82282">
        <property type="entry name" value="SSF82282"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51332">
        <property type="entry name" value="B12_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51337">
        <property type="entry name" value="B12_BINDING_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50970">
        <property type="entry name" value="HCY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50972">
        <property type="entry name" value="PTERIN_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0846">Cobalamin</keyword>
    <keyword evidence="16" id="KW-0170">Cobalt</keyword>
    <keyword evidence="13 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="8 19 24" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="11 19 24" id="KW-0808">Transferase</keyword>
    <keyword evidence="14 19" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Hcy-binding" evidence="20">
        <location>
            <begin position="1"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="Pterin-binding" evidence="21">
        <location>
            <begin position="310"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="domain" description="B12-binding N-terminal" evidence="23">
        <location>
            <begin position="575"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="domain" description="B12-binding" evidence="22">
        <location>
            <begin position="670"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00025552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00031040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="CCZ84432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="790" mass="84887" checksum="A386DD5828BE100F" modified="2013-07-24" version="1">MAQHNLFQKKPRLYLDGGMGTMLQKSGMPAGEIPEKYNIEHPEQITAIHKAFLAAGSDIVYANTFGANRLKMKKSGYSVQELISAGIACGKAATAGTDAMVALDIGPIGQLLEPLGTLKFEEAYDIFREEIEAGKDADLIVIETMTDLYETKAAVLAAKEHSDKPVFVTMTFEENGRTFTGCTVSAMALTLEGLGVDALGVNCSLGPKELLPVVEEICRWTTLPVIVKPNAGLPDPVTGAFSVLPDDFAEAMAAFAKLGVSVFGGCCGTTPEHLAAAYQKLDSMPVVDRPMPEIPPAICSPSVTIPITEPRIIGERINPTGKKRFQAALKANDIDYILEQAVQQTDAGADILDVNVGLPEIDEKDMMIRTIKALQSIGDTPLQLDSTIPEVLEAALRVYNGKPIVNSVNGEEASLQAILPLVKKYGAAVVGLTLDENGIPKKADDRFAIAKRILERAMAMGIPKQDVYIDCLVLTVSAEQDGAVETLKAMRRVHEELGLRTVLGVSNISFGLPNREAVNRTFLTLAMENGLFLPIMNPNIASMTEAVRAYKVLKGIDRSCMQYISSYTGQTSAKPAATAGAADSSMTLAYAMEHGLKKECGSITAKLLETTEPMEIINNMLIPVLDETGTKFEQGKVFLPQLILTADAAQAAFSVIKETMQQRDQAPVSKGKIVLATVKGDIHDIGKNIVKVLLENYGYTVIDLGKDVPCQAVVDAAIEHQVKLVGLSALMTTTLGSMEETIRLLHEQYPSCQTVVGGAVLTPDYAKQIHATFYAKDAKDTVDVAKQVVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5UX20</accession>
    <name>R5UX20_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00663</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000012">
        <property type="protein sequence ID" value="CCZ82858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82858">
        <property type="protein sequence ID" value="CCZ82858"/>
        <property type="gene ID" value="BN566_00663"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ82858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="70" mass="7791" checksum="39EA90A32E6001E9" modified="2013-07-24" version="1">MKQQPNPTDAEQTDALVDMIEQLMENGSGHLVVSEQDSQEGLRVDTYRSMDCAIGNQACCQPTELMDEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXA7</accession>
    <name>R5UXA7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Anti-sigma factor antagonist</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00738</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the anti-sigma-factor antagonist family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000029">
        <property type="protein sequence ID" value="CCZ82938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00738"/>
    <dbReference type="EnsemblBacteria" id="CCZ82938">
        <property type="protein sequence ID" value="CCZ82938"/>
        <property type="gene ID" value="BN566_00738"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043856">
        <property type="term" value="F:anti-sigma factor antagonist activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003658">
        <property type="entry name" value="Anti-sigma_ant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SSF52091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00377">
        <property type="entry name" value="ant_ant_sig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="STAS" evidence="3">
        <location>
            <begin position="16"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ82938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="105" mass="11915" checksum="E1E79F5FD641E9DB" modified="2013-07-24" version="1">MPVTIQLENPCLTAVLDGEIDHHCARSLREEIDQAILDYEPHQLRLDFEKVTFMDSSGIGLILGRCQQMQARSGHVIIQNPPPHIRRVMRLSGMERIASIEITNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXJ5</accession>
    <name>R5UXJ5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ig-like domain-containing surface protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_00791</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000036">
        <property type="protein sequence ID" value="CCZ82998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00791"/>
    <dbReference type="EnsemblBacteria" id="CCZ82998">
        <property type="protein sequence ID" value="CCZ82998"/>
        <property type="gene ID" value="BN566_00791"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026906">
        <property type="entry name" value="LRR_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13306">
        <property type="entry name" value="LRR_5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004394454" evidence="3">
        <location>
            <begin position="30"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="4">
        <location>
            <begin position="651"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="610"/>
            <end position="650"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ82998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="729" mass="76867" checksum="56DB3E5B681CB21D" modified="2013-07-24" version="1">MKTNHWKRTIAGLLAMSTLFSLSFTAVSAEETTAETTAAATTTTTATTVVEIPETDMPTLSVTEEEAALFKVTPLTIKVGEESVKGLSVSGYKGTDTDVVIPDTLIDKKGNELPVFGIADGAFSGKDTITSVKMPETVTTIGANAFSGCSSLTSVNIPDAVTTLGASAFYACTSLASIHIPESITTIDADTFCSCTSLTEIELPSTLIRIGNEAFCSSGLTNITIPEGVTQVGKEAFAGCSNLVSVSIPGSLEVVSASTFQECFRLQSVTFAEGTKKILDSAFYSCPALDNVVLPSTVRWIGNSAFAYSGISNISLPEGLIELASGNFSHCANLKSITLPSTMTCIKSDVFSYSGLESITVPNTITQISSGAFAFCQNLTSVSLPDSLSDVRKQAFYNCTSLTDINIPASMTLLEAQTFQNCTSLTNVTLPDGLVKIEDYVFYNTGITSITIPDSVTSLGISAFSNCASLESATLGNGLTKVDKWLFRNCSSLKDVTLGENIQKVDNFAFAECGNLETITLPDSVTNIGISAFEKCRSLDDVKLPDGLTTVDKNAFLDCDKLTNVTIPDSVTTIGNQAFGYQTNNDMSTSKKDNFQITGKTGSAAADYANNSGISFNDPDAPTTTTTTDTTDVSGETTETTTVTTDSGTSSENICGDTTMDGKVDLVDAVLLNKFLAGAVTFTDQQVTNANCDQTDGTDTVGEEDTTALIRFVLNMEGYQTLPHISENA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXJ9</accession>
    <name>R5UXJ9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00605</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000008">
        <property type="protein sequence ID" value="CCZ82800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82800">
        <property type="protein sequence ID" value="CCZ82800"/>
        <property type="gene ID" value="BN566_00605"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="85" mass="8605" checksum="C7057AC1A870917C" modified="2013-07-24" version="1">MNEKKNPLQDRSLLVGLLALVISLLSGILLFLHVPVLVCAILAIIGTLGGIASLLLGKSGALPAAVAIGTGLMCVLTFAVENAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXL4</accession>
    <name>R5UXL4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted phosphohydrolases</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00620</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000010">
        <property type="protein sequence ID" value="CCZ82815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00620"/>
    <dbReference type="EnsemblBacteria" id="CCZ82815">
        <property type="protein sequence ID" value="CCZ82815"/>
        <property type="gene ID" value="BN566_00620"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos" evidence="2">
        <location>
            <begin position="48"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ82815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="285" mass="32138" checksum="8936F34E78F86274" modified="2013-07-24" version="1">MKGILIFIAAAVFLLFLFFLCSMLERRRLVIRKQSLHLSQLPQAFDGLRLVQLSDLHKQVYPHGERKLLQAVQACQPDVIVITGDLVSRTVMDFSERQELLTHLRQIAPVYLCLGNHEWDMQPQQIADYRAMIAAAGCELLENQTVSFHRGAETCYLAGASLRIGIYHNADFQYQDLETYSPEDLTHDIGNRRGCTILLAHSPFPAESYFAWGADLTLSGHVHGGIVRIPGIGGLLSPERKLFPKYSKGLYTKDGKYLYVNCGIGKLRLGNPPEVTMLELHSKWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXM7</accession>
    <name>R5UXM7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">Histidinol-phosphatase</fullName>
            <shortName evidence="7">HolPase</shortName>
            <ecNumber evidence="4 7">3.1.3.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN566_00831</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>H2O + L-histidinol phosphate = L-histidinol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14465"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57699"/>
            <dbReference type="ChEBI" id="CHEBI:57980"/>
            <dbReference type="EC" id="3.1.3.15"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 8/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the PHP hydrolase family. HisK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.15" evidence="4 7"/>
    <dbReference type="EMBL" id="CBAR010000040">
        <property type="protein sequence ID" value="CCZ83038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00831"/>
    <dbReference type="EnsemblBacteria" id="CCZ83038">
        <property type="protein sequence ID" value="CCZ83038"/>
        <property type="gene ID" value="BN566_00831"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00013"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004401">
        <property type="term" value="F:histidinol-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010140">
        <property type="entry name" value="Histidinol_P_phosphatase_HisJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004013">
        <property type="entry name" value="PHP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21039">
        <property type="entry name" value="PTHR21039"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02811">
        <property type="entry name" value="PHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="SSF89550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01856">
        <property type="entry name" value="hisJ_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PHP" evidence="8">
        <location>
            <begin position="5"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ83038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="255" mass="29032" checksum="F35E77BB56F2A3A1" modified="2013-07-24" version="1">MFANYHTHTARCHHATGEDKAYVEAAIQNGFQVLGFSDHCPWIFSDGYVSPIRMTPDELDGYVTSLTALKKEYAADITIYIGLESEYVPEMLPEQQKFLQDYPIDYQILGEHFLYPEQRGIYTGKPTDNAAELKHYVDQVIAGMETGNYLYLAHPDLFHFIGDPACYEQEFRRLCVYLKEKQIPIEINQLGLADHRQYPNPAFLKIAESVGNTAIIGCDAHHPSALLDTDSQQACKKLAEQYHLPLMEVLPQLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXN3</accession>
    <name>R5UXN3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00841</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000042">
        <property type="protein sequence ID" value="CCZ83048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83048">
        <property type="protein sequence ID" value="CCZ83048"/>
        <property type="gene ID" value="BN566_00841"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004394456" evidence="1">
        <location>
            <begin position="30"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="218" mass="24525" checksum="D3FF60395863B9D2" modified="2013-07-24" version="1">MKNRWKQWFCCLFAVILALCSLTACGSKKEKGNVSMEDLPFGAVLRDLNTTEIPVEYDSRYFTEEAMTVLSNYFYAIQTEDTALYQEVTLDFYDDYVMQQMFQGLVGLDGLLTQLKSSYTPQQSATGTSTENADADVTFTQINVVECEQSTDSVHSNLDNLYPMLNELSGEEDYCTQHVSDAKFLNLSLSLKSGDNTTTLSDQYVFLLCVDGKYVICS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXN5</accession>
    <name>R5UXN5_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glycerol-3-phosphate dehydrogenase [NAD(P)+]</fullName>
            <ecNumber evidence="7">1.1.1.94</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">NAD(P)H-dependent glycerol-3-phosphate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">gpsA</name>
        <name evidence="15" type="ORF">BN566_00640</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>NAD(+) + sn-glycerol 3-phosphate = dihydroxyacetone phosphate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:11092"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.94"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 12">
            <text>NADP(+) + sn-glycerol 3-phosphate = dihydroxyacetone phosphate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:11096"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.94"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 11">Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.94" evidence="7"/>
    <dbReference type="EMBL" id="CBAR010000012">
        <property type="protein sequence ID" value="CCZ82835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00640"/>
    <dbReference type="EnsemblBacteria" id="CCZ82835">
        <property type="protein sequence ID" value="CCZ82835"/>
        <property type="gene ID" value="BN566_00640"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009331">
        <property type="term" value="C:glycerol-3-phosphate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004367">
        <property type="term" value="F:glycerol-3-phosphate dehydrogenase [NAD+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046167">
        <property type="term" value="P:glycerol-3-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046168">
        <property type="term" value="P:glycerol-3-phosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006650">
        <property type="term" value="P:glycerophospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00394">
        <property type="entry name" value="NAD_Glyc3P_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006168">
        <property type="entry name" value="G3P_DH_NAD-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006109">
        <property type="entry name" value="G3P_DH_NAD-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011128">
        <property type="entry name" value="G3P_DH_NAD-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07479">
        <property type="entry name" value="NAD_Gly3P_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01210">
        <property type="entry name" value="NAD_Gly3P_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000114">
        <property type="entry name" value="Glycerol-3-P_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00077">
        <property type="entry name" value="GPDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 7" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="4 7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 10 11" id="KW-0520">NAD</keyword>
    <keyword evidence="3 7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5 7" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD_Gly3P_dh_N" evidence="13">
        <location>
            <begin position="4"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="NAD_Gly3P_dh_C" evidence="14">
        <location>
            <begin position="178"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7 9">
        <location>
            <begin position="253"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7 8">
        <location>
            <position position="189"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7 10">
        <location>
            <begin position="8"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7 10">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 10">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000114-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000114-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000114-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF07479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CCZ82835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="334" mass="35635" checksum="F1C75608F5FB986B" modified="2013-07-24" version="1">MEHITILGSGGFGLSLALSAYRSGHAVKVWSKFPEELEAIRRDGEHKQKLPGVPIPSEITLTADLEAAISGADLVIFGIPSSFLRATAKLAAPYLHTPMVIVNTGKGLEAGTHYTMSQILQEELPHLPIVTITGPSHAEEVARNVPTTVVAASKDLQAAEYVQKTMSSETLRLYRNSDIIGCEVGGALKNIIALSAGICDGLGCGDNTKAALMTRGMHEITKLGVAMGGQRETFAGLSGIGDLIVTCCSMHSRNRRAGILIGEGVSPEEAVQQVGTVEGYHCCEVAYALAQQMGVSMPITEQLNQVLFHGGNPKDAIYALMCRPQRSEWVEQEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-09-29" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXR0</accession>
    <name>R5UXR0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Bacterial Ig-like domain group 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_00667</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000014">
        <property type="protein sequence ID" value="CCZ82865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82865">
        <property type="protein sequence ID" value="CCZ82865"/>
        <property type="gene ID" value="BN566_00667"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003343">
        <property type="entry name" value="Big_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008964">
        <property type="entry name" value="Invasin/intimin_cell_adhesion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019931">
        <property type="entry name" value="LPXTG_anchor"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02368">
        <property type="entry name" value="Big_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00746">
        <property type="entry name" value="Gram_pos_anchor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49373">
        <property type="entry name" value="SSF49373"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0134">Cell wall</keyword>
    <keyword evidence="3" id="KW-0572">Peptidoglycan-anchor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0964">Secreted</keyword>
    <keyword evidence="2 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004406533" evidence="4">
        <location>
            <begin position="26"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="domain" description="BIG2" evidence="6">
        <location>
            <begin position="70"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="GRAM_POS_ANCHORING" evidence="5">
        <location>
            <begin position="526"/>
            <end position="565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ82865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="565" mass="62059" checksum="F40D9F125E0E32A0" modified="2013-07-24" version="1">MKKWAFLVAMMLLTGSLSTLFPVSATDVPVDTEVTTTLTEIQETTETTTSPAITEPIEDPSSTEVVASDFSVEPSVCNLTVGESCNLNVKFDKEGYDTEHIEYQSADESIATVSKYGLVTAISAGETCIRIFVFDEADNPVKTIPIPVVIAPDDTMSTEQQEQLAALKEKGSHLFGEFIREEKIILGELPPDTARLCFDDVQEILANNTDFQNIYESLLSIAKYPDFVGGSGVTLVEFWLDDYGTEKILLILEQEDVISVKCNADGNISDWKPLYSEEKAETTATTATTETTTTTATVTSDALSEEQAKALAELAEKEHGVFGEFERARASILNKIPEDASRMTLEEAKEFVNSSDSFKEIYDKLSASQPYPDFIGGSGVTKVEYWFDDKGKEKILLILEQEDVIYVDCTKQGDVSKWERLYPVSDVEDSNIKDSNLAGTYKIYNGIEDTVTTESTTTNYKNITQEDFKDWAMKDYATKTGNAPAKAALLETADGNYAITLTDEDGEVLDVYTVNPETATGTNMANEVVSLPQTGYSDIYKVLVGLATLMTVGGGTLIVKTREKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXU0</accession>
    <name>R5UXU0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00925</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000062">
        <property type="protein sequence ID" value="CCZ83138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83138">
        <property type="protein sequence ID" value="CCZ83138"/>
        <property type="gene ID" value="BN566_00925"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="35" mass="4063" checksum="0957C72273A157B0" modified="2013-07-24" version="1">MSDFLWYAKRYAAQTLQEVEQDVRISITRTLSANG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXV3</accession>
    <name>R5UXV3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Spore surface glycoprotein BclB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00940</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000064">
        <property type="protein sequence ID" value="CCZ83153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00940"/>
    <dbReference type="EnsemblBacteria" id="CCZ83153">
        <property type="protein sequence ID" value="CCZ83153"/>
        <property type="gene ID" value="BN566_00940"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="155" mass="16083" checksum="8E1A71BD2949D45F" modified="2013-07-24" version="1">MLNFVIPAGATGASAAESRPSVLAVQDTTEQALTENTPVFFAVNLLQSGTDLTHAAGSNQITVETPGIYQAAFTAEVSPTGTATLPLTISLSLEQGTTILETSSTTMTTAEQSNTLAWNIPFTVTTAPEVLQILSDGTDVILNRATLTINKIGTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXZ7</accession>
    <name>R5UXZ7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">30S ribosomal protein S18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rpsR</name>
        <name evidence="5" type="ORF">BN566_00981</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Binds as a heterodimer with protein S6 to the central domain of the 16S rRNA, where it helps stabilize the platform of the 30S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Part of the 30S ribosomal subunit. Forms a tight heterodimer with protein S6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the bacterial ribosomal protein bS18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000070">
        <property type="protein sequence ID" value="CCZ83198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00981"/>
    <dbReference type="EnsemblBacteria" id="CCZ83198">
        <property type="protein sequence ID" value="CCZ83198"/>
        <property type="gene ID" value="BN566_00981"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00270">
        <property type="entry name" value="Ribosomal_S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001648">
        <property type="entry name" value="Ribosomal_S18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036870">
        <property type="entry name" value="Ribosomal_S18_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13479">
        <property type="entry name" value="PTHR13479"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01084">
        <property type="entry name" value="Ribosomal_S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00974">
        <property type="entry name" value="RIBOSOMALS18"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46911">
        <property type="entry name" value="SSF46911"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00165">
        <property type="entry name" value="S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3 4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="79" mass="9218" checksum="796B8B3781D9F094" modified="2013-07-24" version="1">MERAPRMGGKRPRKKVCMFCVDRVDVIDYKDIAKLRKCMTERAKILPRRVTGTCAYHQRKLTTAIKRARHVALLPYVSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYB7</accession>
    <name>R5UYB7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00803</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000038">
        <property type="protein sequence ID" value="CCZ83010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83010">
        <property type="protein sequence ID" value="CCZ83010"/>
        <property type="gene ID" value="BN566_00803"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="149" mass="16376" checksum="BBABC0FA4D59F6B5" modified="2013-07-24" version="1">MDVNGEYPDETIEKVVEKSVYTRRIGDILPDNLVDLDDAVNLLGIVSFLDSCTAIGAAYTAFLNDAMTADYSYTLQDGTTGTYRERFKDSLYANSVPFAEIADCDQNGRIEKNDADLLVTYYAQAAAGQSPESLINNELSKVVYADITM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYD0</accession>
    <name>R5UYD0_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Putative manganese efflux pump MntP</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">mntP</name>
        <name evidence="10" type="ORF">BN566_00818</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Probably functions as a manganese efflux pump.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cell membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the MntP (TC 9.B.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000040">
        <property type="protein sequence ID" value="CCZ83025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00818"/>
    <dbReference type="EnsemblBacteria" id="CCZ83025">
        <property type="protein sequence ID" value="CCZ83025"/>
        <property type="gene ID" value="BN566_00818"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01521">
        <property type="entry name" value="MntP_pump"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003810">
        <property type="entry name" value="Mntp/YtaF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022929">
        <property type="entry name" value="Put_MntP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35529">
        <property type="entry name" value="PTHR35529"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02659">
        <property type="entry name" value="Mntp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0464">Manganese</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="36"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="68"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="138"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="172"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ83025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="195" mass="21161" checksum="5BCD8D1AEC3E200F" modified="2013-07-24" version="1">MGIGEICLLGVGLSMDAFAVSICKGLQMRKFNWKQALWMGIFFGGFQALMPLTGWLLGALFAKHIMQYSHWIAFVLLAFIGGKMLLESIRQKKEEPSEETAVCPNKVDFRELFLLAVATSIDALAVGVTFSIMPDVNIFLSILLIGSITFLFALAGVKIGNLFGDRFEKQTGILGGCILLAIGVKILLEGLGIIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYD1</accession>
    <name>R5UYD1_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Stage 0 sporulation protein A homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN566_01114</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000089">
        <property type="protein sequence ID" value="CCZ83333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01114"/>
    <dbReference type="EnsemblBacteria" id="CCZ83333">
        <property type="protein sequence ID" value="CCZ83333"/>
        <property type="gene ID" value="BN566_01114"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Response regulatory" evidence="7">
        <location>
            <begin position="2"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="8">
        <location>
            <begin position="124"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="6">
        <location>
            <begin position="124"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="5">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ83333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="225" mass="25643" checksum="AAF69D86E078B358" modified="2013-07-24" version="1">MRLLVVEDEQSLREDITKKLQHSGYEVDACGDGETASEYLAVEYYDLVLLDLNLPKIDGMMLLRNFRKENQETPVLILSARSEINDKVEGLDAGANDYLSKPFHLAELEARIRSLTRRQFIQRDVCLQCGKITYDTKSRIATVDGVAINLTRKETGVLEYLMLHQGRPVSQEELIEHVWDSSVDSFSNSIRVHISALRKKLRAVLGYDPIRNRIGEGYEIEGESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYT9</accession>
    <name>R5UYT9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00129</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000113">
        <property type="protein sequence ID" value="CCZ83473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83473">
        <property type="protein sequence ID" value="CCZ83473"/>
        <property type="gene ID" value="BN566_00129"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="211"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="264"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="290" mass="31879" checksum="132B2B35580324DE" modified="2013-07-24" version="1">MTNGFGKRLVVRLLLTILLTFTVLGTLGSGYAGYLLSSPKVCLSQVEKQQAAAKIHDSVESYFTAQYQTTAIPAETYLNVVTEDWIAERLEEQITSEYQILNGAKLSYQLDMSDLEAALTQFFSDYAEEINYTKDSTYEQKLQETIDNAETELTSQLDVYHFSTMRNANILSRLTGKQLYLWIACGVCGVVSLLLMGILMGLERNGEWGMLYFPGCALFINGVFLILPTAWVLATSYFSGLAIKVPAVYAAVTGVLYHCTRVGLVAGIVLAVLGLIAIVGSTIGRRKAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYY3</accession>
    <name>R5UYY3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01267</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000118">
        <property type="protein sequence ID" value="CCZ83498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83498">
        <property type="protein sequence ID" value="CCZ83498"/>
        <property type="gene ID" value="BN566_01267"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="145" mass="17391" checksum="C5894DC0432F0D66" modified="2013-07-24" version="1">MRICSFPLDGAAIPTIHPEDVYLIKMADEQSMELFVMQEDLLTTYYTCHMSPYLWKRIKRAFSISKNTSNDLWEWKSSGRGDDWYWKRSIPTQYQKELERLILLDQFWAKQWMLQLAFCPSALRLLHQLARKKNPCAPFYKVYRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZ32</accession>
    <name>R5UZ32_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SUF system FeS assembly protein NifU family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01091</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01091"/>
    <dbReference type="EnsemblBacteria" id="CCZ83310">
        <property type="protein sequence ID" value="CCZ83310"/>
        <property type="gene ID" value="BN566_01091"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06664">
        <property type="entry name" value="IscU_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002871">
        <property type="entry name" value="NIF_FeS_clus_asmbl_NifU_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10093">
        <property type="entry name" value="PTHR10093"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01592">
        <property type="entry name" value="NifU_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NifU_N" evidence="1">
        <location>
            <begin position="30"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="142" mass="15734" checksum="0DCE519C00BA4733" modified="2013-07-24" version="1">MANNFYNEILTEHNVRPEFKYDLPDATIKLEGVNPNCGDDIFLNLKLEDGIIQDASFCGDGCAISQASADIMIGMIVGKTKEEALHLSDLFLKMIKGTATEEEVDELEEASALQDISHMPARVKCAVLGWRTLREALEKTTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZ83</accession>
    <name>R5UZ83_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4368 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01121</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000092">
        <property type="protein sequence ID" value="CCZ83345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83345">
        <property type="protein sequence ID" value="CCZ83345"/>
        <property type="gene ID" value="BN566_01121"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025378">
        <property type="entry name" value="DUF4368"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14287">
        <property type="entry name" value="DUF4368"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4368" evidence="2">
        <location>
            <begin position="24"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="4"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="94" mass="10778" checksum="BC929BE8A7422195" modified="2013-07-24" version="1">MELSADYEAEQAELKKRAAALQAELDKSQAATVNAEKFMGIVRKHLAFEELTPTLLREMIEKIVVHECSYDENGTRRQDIEIYYSFVGKIDLPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZC2</accession>
    <name>R5UZC2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted phosphohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01424</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000153">
        <property type="protein sequence ID" value="CCZ83688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01424"/>
    <dbReference type="EnsemblBacteria" id="CCZ83688">
        <property type="protein sequence ID" value="CCZ83688"/>
        <property type="gene ID" value="BN566_01424"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014578">
        <property type="entry name" value="Pesterase_CT488"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF033094">
        <property type="entry name" value="Pesterase_CT488"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos" evidence="2">
        <location>
            <begin position="3"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="229" mass="26526" checksum="C2D1C451E2EA81E1" modified="2013-07-24" version="1">MQLYGIGDLHLSLGVPEKPMDIFHGWENYMQLLEQHWRAMVQPEDTVVLAGDLSWGMTLPQAKADFAFLETLPGQKIILKGNHDYWWNSMKKMQQFFADNGFDSLHILHNNHYAFQQYGICGTRGWVSINGETADAKVLAREVQRLEVSIQSALKEQLTPIVFLHYPPIYGNSYNYEILEVLHRYAITECYYGHIHGKAHQYAFEGVYEETKMHLISGDYIQFKPVKIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZQ4</accession>
    <name>R5UZQ4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Spore germination B3/ GerAC like C-terminal</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_01568</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GerABKC lipoprotein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000165">
        <property type="protein sequence ID" value="CCZ83833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83833">
        <property type="protein sequence ID" value="CCZ83833"/>
        <property type="gene ID" value="BN566_01568"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009847">
        <property type="term" value="P:spore germination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008844">
        <property type="entry name" value="Spore_GerAC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05504">
        <property type="entry name" value="Spore_GerAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0309">Germination</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004406602" evidence="4">
        <location>
            <begin position="22"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="308" mass="34051" checksum="F70A5CB6BC8E26A5" modified="2013-07-24" version="1">MKKSLLSLLLCLSLCCSGCSATQVKDRLYLQSLELSNYRYPTVQLHAFQSEEADCSGSGSTLSAALESAEIPAGKSLFLGHLELLALQEPKFLQQLPDLLQQYRLSSGCKLLYTTRSLAEVNTETLLESLKQEEQNGRIPETDLLSILKERCNSSGTALLPFRITNGFSMVLIASNGTTQTLSNDAIQGLCWLRGNNYPERVSLSTDGQASDFLITSASTRYTAITANGIPIVTVSISIHGTGNAIALRERLEIACEAAERETIQQNQADVIELEACLRQQCYKLYQEQDWNSLLRTVQFQHEFTILP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZQ7</accession>
    <name>R5UZQ7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator DeoR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_01254</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000117">
        <property type="protein sequence ID" value="CCZ83485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01254"/>
    <dbReference type="EnsemblBacteria" id="CCZ83485">
        <property type="protein sequence ID" value="CCZ83485"/>
        <property type="gene ID" value="BN566_01254"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014036">
        <property type="entry name" value="DeoR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001034">
        <property type="entry name" value="DeoR_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018356">
        <property type="entry name" value="Tscrpt_reg_HTH_DeoR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00455">
        <property type="entry name" value="DeoRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08220">
        <property type="entry name" value="HTH_DeoR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00037">
        <property type="entry name" value="HTHLACR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00420">
        <property type="entry name" value="HTH_DEOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00894">
        <property type="entry name" value="HTH_DEOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51000">
        <property type="entry name" value="HTH_DEOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH deoR-type" evidence="4">
        <location>
            <begin position="3"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="248" mass="27406" checksum="32B319E019FA679A" modified="2013-07-24" version="1">MLAEERHALILDLVNQQGNVKLTELCSRLHASQSTIRRDLLELSEQGLLTKVHGGAISRNERFFPVEHNMEEKANLYIAEKTAIARYAASLLEEGDFVYLDAGTTTEKVIDFIPLKKITFVTNGFLHAKRLAQRGFQVYIPAGAVKAATEAIVGAECVLSLQSYNFTKCLMGVNGISLTAGLSTPDKGEASVKKAAIHQSKQVYFLADHSKFDQISAITFAHLNGVQIITDRLPDQKYRSEATIREVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZT0</accession>
    <name>R5UZT0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01593</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000167">
        <property type="protein sequence ID" value="CCZ83858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83858">
        <property type="protein sequence ID" value="CCZ83858"/>
        <property type="gene ID" value="BN566_01593"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010001">
        <property type="entry name" value="BofA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07441">
        <property type="entry name" value="BofA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="84" mass="9258" checksum="88C372000303BCAD" modified="2013-07-24" version="1">MEQIVFWAIWAALAVFMGYFYLHHAHGIRSFLRGTGTGLVALLLLHELGGWIGFAPEVNGFNLMQAGILGVPGVLLMMAVHFFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZW7</accession>
    <name>R5UZW7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 7">Transcriptional regulator MraZ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">mraZ</name>
        <name evidence="10" type="ORF">BN566_01627</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="7">Forms oligomers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
            <location evidence="7">Nucleoid</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the MraZ family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000171">
        <property type="protein sequence ID" value="CCZ83898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01627"/>
    <dbReference type="EnsemblBacteria" id="CCZ83898">
        <property type="protein sequence ID" value="CCZ83898"/>
        <property type="gene ID" value="BN566_01627"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009295">
        <property type="term" value="C:nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16321">
        <property type="entry name" value="MraZ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16320">
        <property type="entry name" value="MraZ_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1550.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01008">
        <property type="entry name" value="MraZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003444">
        <property type="entry name" value="MraZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035644">
        <property type="entry name" value="MraZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020603">
        <property type="entry name" value="MraZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035642">
        <property type="entry name" value="MraZ_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038619">
        <property type="entry name" value="MraZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007159">
        <property type="entry name" value="SpoVT-AbrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037914">
        <property type="entry name" value="SpoVT-AbrB_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34701">
        <property type="entry name" value="PTHR34701"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02381">
        <property type="entry name" value="MraZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89447">
        <property type="entry name" value="SSF89447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00242">
        <property type="entry name" value="TIGR00242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51740">
        <property type="entry name" value="SPOVT_ABRB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SpoVT-AbrB" evidence="9">
        <location>
            <begin position="7"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="SpoVT-AbrB" evidence="9">
        <location>
            <begin position="77"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ83898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="142" mass="15878" checksum="52C5CF51CE2340B3" modified="2013-07-24" version="1">MTSLIGTYYHVIDAKGRMNFPTKLRELLGSSFYVTKGLDAHCLAVYPAEEWERLSQKVAAIPESKGAQLKRWLFSGACEMVPDQQGRILIPQDLRQFAGLEKDVVVIGANNKAEIWNRASWETANAAVDLDEMMSILDTLGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZZ0</accession>
    <name>R5UZZ0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01309</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000121">
        <property type="protein sequence ID" value="CCZ83540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83540">
        <property type="protein sequence ID" value="CCZ83540"/>
        <property type="gene ID" value="BN566_01309"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="182"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="213" mass="23856" checksum="EF994235A158890D" modified="2013-07-24" version="1">MTGWLIAGGILLLLAGLLFTPAVLHLTLQDNAPPVIRLSFLGIPLYRSDRKQHAAKRSAKHKKKRTKKQTSSSKKKKQKQTEEERRSLSEWFDLLHDACSSICKGLRRLTKCIRFRHVSIYIGVGNWDAAACAAEYAKLNTALYPCLAVLGTVCSVAYDGIDVRCCFGQNRTRYYLSGTVRLSLANVLAAAFAFLFSFLWNTLERKQAPSEQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>R5V008</accession>
    <name>R5V008_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-binding protein Hfq</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hfq</name>
        <name evidence="2" type="ORF">BN566_01333</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">RNA chaperone that binds small regulatory RNA (sRNAs) and mRNAs to facilitate mRNA translational regulation in response to envelope stress, environmental stress and changes in metabolite concentrations. Also binds with high specificity to tRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homohexamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Hfq family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000131">
        <property type="protein sequence ID" value="CCZ83565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01333"/>
    <dbReference type="EnsemblBacteria" id="CCZ83565">
        <property type="protein sequence ID" value="CCZ83565"/>
        <property type="gene ID" value="BN566_01333"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01716">
        <property type="entry name" value="Hfq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00436">
        <property type="entry name" value="Hfq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005001">
        <property type="entry name" value="Hfq"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34772">
        <property type="entry name" value="PTHR34772"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17209">
        <property type="entry name" value="Hfq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="SSF50182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02383">
        <property type="entry name" value="Hfq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0346">Stress response</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="79" mass="9014" checksum="1163AE603F537C3A" modified="2013-07-24" version="1">MNKMMNLQDVFLNQARKEKILVTIILMNGFQFKGIVKGFDSYIVILECNGEQNLVYKHAISTLKPTRPIAILDAAEKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5V015</accession>
    <name>R5V015_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transketolase N-terminal subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01343</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000131">
        <property type="protein sequence ID" value="CCZ83575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01343"/>
    <dbReference type="EnsemblBacteria" id="CCZ83575">
        <property type="protein sequence ID" value="CCZ83575"/>
        <property type="gene ID" value="BN566_01343"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02012">
        <property type="entry name" value="TPP_TK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005474">
        <property type="entry name" value="Transketolase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00456">
        <property type="entry name" value="Transketolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00801">
        <property type="entry name" value="TRANSKETOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TRANSKETOLASE_1" evidence="1">
        <location>
            <begin position="17"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS00801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="279" mass="30335" checksum="5AB482944E99E115" modified="2013-07-24" version="1">MEEITKKKLQAIACKVRMGVIEGTYCAKSGHPGGSLSISDLLTYLYFAKMHIDPKQPEMADRDRLVLSKGHCAPALYATLAQRGFFPQEELQSLRHIGAMLQGHPCIHTPGIDMSSGSLGQGISVACGMALAGKLDNAAYKVYTILGDGEIEEGQVWEAAMFASHYHLDNLLAIVDYNGLQIDGQLSEVCSPEPIPEKFAAFGWHVIQMDAHDFDSIEAAFAEAEQIVGQPVVIVQKSVKGKGVSFMENQVGWHGKAPNQAEYEQAMAELTAQLQELEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5V053</accession>
    <name>R5V053_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01697</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000177">
        <property type="protein sequence ID" value="CCZ83968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83968">
        <property type="protein sequence ID" value="CCZ83968"/>
        <property type="gene ID" value="BN566_01697"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014245">
        <property type="entry name" value="Spore_III_AF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09581">
        <property type="entry name" value="Spore_III_AF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="161" mass="17823" checksum="7CE0F0DE9C8CCCE6" modified="2013-07-24" version="1">MERLQVAVLAACVMSMVTGMLQLLRPNEKFDRQLRLLTAGMMLFSILIPLMQGISEIQPAWNFLEPQSVEEESSITEQVQEQLTTQIEQNLEQNLQVSLETHGIVVTDLTVCAHISEEQRIDISSVHVVCDAPGAAKQLLQADLGEEVHLIVEAATSTAEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0E7</accession>
    <name>R5V0E7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01480</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000161">
        <property type="protein sequence ID" value="CCZ83745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01480"/>
    <dbReference type="EnsemblBacteria" id="CCZ83745">
        <property type="protein sequence ID" value="CCZ83745"/>
        <property type="gene ID" value="BN566_01480"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="879"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="919"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="954"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="984"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1075"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1105"/>
            <end position="1128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1390"/>
            <end position="1410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1416"/>
            <end position="1434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="556"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="683"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="211"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="317"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="359"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="517"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="716"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="742"/>
            <end position="756"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="1439" mass="162286" checksum="94F557B384D043B2" modified="2013-07-24" version="1">MANQKFSVEEILKEYQSDEATKGKKESPSGKLETQKMLNHAAGRRNVPPSTEASAEAYSYASSRTRQRTPYSPYRSEMAANINQIKQNRYQRRTDVEESRTAAFQTLELMRPKVSFVRSPAIRPQTTPNPKSDHISDYDGAVLLEQPESEQKPEQPVYQPAIQEMQDSTRAKEKKQAQKSRKPKKRTEQSYQRESLTEPEPEWRKAKTETFPAITPTQTLQETLREAQEAVAKAEAEAAAAVAALKAEQQQPQKKQPISHAFETLLTSPAKQQEKQQKQQQEKKQPVAKRWFSGKKRVPQVNHTAPPSYPEPAEAYRQPVEPQPKPYTPPVQKVEEPKPYTPPVQKVEEPKPYMPPVRKVEEPKPYTPPVQKVEEPKPYTPPVQKVKEPKPYTPPVQKVKEPKPYTTPVKKVEEPKPYTSPVQKVEEPKPYTSPVQKAEEPKPYTPPVQKVEEPKPYTPLVQKMEEPKPHTPPVQKVEEPKPYTPSVQKVEEPKPYTPPVKKVEEPKPYTPPVQEPVQEEVTVSTTSEPLESTPEQKPKKKKWWQWWKKETPSVLKFAQPEESYMQPVQEEPVQPETSYIPPVQEPAQIEESPIITEPVEPTPVQKPKKQKKARKKKEKPSKQQFAQPEESYAPPVQEYTQPEEAYAPPVQEYTQPEEAYMPPVQEYMQPEEPYTPPVQEYTQPEEAYMPPVQEYMQPEESPVATESELVKSTPIQKPKKQKKVRQKKEKKPKKQKSIPVEESTPVPVTNDVPNSESEFAAGYAPAEQPLETDWTTEESSHSGWWDDSESKIPGSSGFDPTEEPAPQPEENESYTARFKKVVVQESTYTEPEQPKKPRKPKKEKTPRVKVQKPVPPEPKSIPNQEAEIRVNINLLRNAVFFRTVSLAVLTVIGAFLAIGESSYTLLYPALSQLMTVRGYCFLHLILGLLILFITFPTVINGMRKLFHREADSDTIAAMPLIPCLLTVLATIFVPEPMQHNLVHIFLPVSSFILLMNSIGKLFIIRRATRNFALLTKNFEKYIVTCVQSETAAEELTRGVVQDYPILATIRKTKQMSDFLRHTYASDLADRFSKKLAPIVAGISLLLAAAITGIRIGVLETPAENIPFFCSVLTMLLTAGCSAGIPLTVNLPLDRASKRLCPHGCTLLGYQSVDDFYDVNSILVPAATLFPKQSITISGIKNFSGFQVEEALLDAASMVYAADSILQNAFAALVEEKDGGMLYQVEDCSYEDNLGICGWIRHRRVLLGNREMMMAHHVEGMPTKVRELEMTSGSQDVLYLSVSGVVAGMFVIDITADPMVKRQMQRLKEEQISVIVKSVDSCITLQRLTTLFGVPDSMLKILPSADNDLYEKETAPLQDVSASTIHDGSFFGTARLLLEARSIKRAATTGLLLQVIAVILGLSICIGYILVNGYDNITSQTLLFFQLVMTGLTLLTTRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0S3</accession>
    <name>R5V0S3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Heavy metal transport/detoxification protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01599</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000169">
        <property type="protein sequence ID" value="CCZ83870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01599"/>
    <dbReference type="EnsemblBacteria" id="CCZ83870">
        <property type="protein sequence ID" value="CCZ83870"/>
        <property type="gene ID" value="BN566_01599"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="SSF55008"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMA" evidence="1">
        <location>
            <begin position="2"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="72" mass="8104" checksum="294BD201CC2070EF" modified="2013-07-24" version="1">MKKTFKLQDLDCANCAAKMERAIQQVEGVISANVSFMTQKLSIEIADENFDSVWKEVKKKAKKANPDVTILD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1K2</accession>
    <name>R5V1K2_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ATP-dependent zinc metalloprotease FtsH</fullName>
            <ecNumber evidence="7">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ftsH</name>
        <name evidence="11" type="ORF">BN566_01870</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
            <orientation evidence="7">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">In the central section; belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="7"/>
    <dbReference type="EMBL" id="CBAR010000192">
        <property type="protein sequence ID" value="CCZ84145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01870"/>
    <dbReference type="EnsemblBacteria" id="CCZ84145">
        <property type="protein sequence ID" value="CCZ84145"/>
        <property type="gene ID" value="BN566_01870"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01458">
        <property type="entry name" value="FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005936">
        <property type="entry name" value="FtsH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="SSF140990"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01241">
        <property type="entry name" value="FtsH_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 7 11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 7 11" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="115"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="AAA" evidence="10">
        <location>
            <begin position="198"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="607"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="616"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="429"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="206"/>
            <end position="213"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="428"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="432"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="504"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ84145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="637" mass="70890" checksum="BB8B36BA7FB71257" modified="2013-07-24" version="1">MTPKKSKGIGAYLLVVVLLLLGVFFITQNIGGKENDTQYSEIMNYFDDYDVKKYTLNLGNGQLKMTLQDNRTITYEVPYISGFLDDVKDYRQEYNAKHPDAPLQMDYYKMKDSSWIWTLLPTILLLVMGILLFVFMMRQANGGGKYTSFGKANIKNQANTRKATFADVAGADEEKHELEEIVDFLKNPKKYTEIGARIPKGVLLLGPPGTGKTLLARAVAGEAGCPFFSISGSDFVEMFVGVGASRVRDLFEQAKKNAPSIIFIDEIDAVGRHRGAGLGGGHDEREQTLNQLLVEMDGFTGKDNVIVIAATNRRDILDPALLRPGRFDRQIVVGYPDVKGREAILRVHTKKRPLAPDVDLATIAKSTVGFTGADLENLVNEASLLAARQNKRAITMQEIQEATIKVIAGPEKKSHKVSEKEKRLTAYHEAGHAVCTYYCDHQDKVHQVSIIPRGMAGGYTMSLPEQDKSFVSKTEMEENIVTLLGGRVAEQLVLDDISTGASNDLERATATARKMVTRYGFSKKLGPIVYGNDQNEVFLGKDLGSSRDYSDRVAGEIDDEVRNIVETAYQKATILLQAHMDQLHLLSKYLILHEKIERDDFEKLMQGKMEASAFEEQPTAPQQPESSSEEQNLPEQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5V2A0</accession>
    <name>R5V2A0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative lipoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00526</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000267">
        <property type="protein sequence ID" value="CCZ84673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84673">
        <property type="protein sequence ID" value="CCZ84673"/>
        <property type="gene ID" value="BN566_00526"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="180" mass="19269" checksum="9ECEF4578EE886D4" modified="2013-07-24" version="1">MNPKKIGITIASALFVLIGGITGVQYLQDSPEESSSSDALTASGSETTVAATETYQTTAATETTTVSMQTTAPAQTESAPAMTLVSDAKGASYYTFRTDALLESHYEKHGIEMGFDSAAAYEAAANAVIANPEVLHKYESEDGDDVYYLADTNEFVVVSVDGYLRTYFEPSAGMAYYERQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>R5V2E3</accession>
    <name>R5V2E3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phosphate transport system permease protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN566_00374</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Part of the binding-protein-dependent transport system for phosphate; probably responsible for the translocation of the substrate across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000238">
        <property type="protein sequence ID" value="CCZ84475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00374"/>
    <dbReference type="EnsemblBacteria" id="CCZ84475">
        <property type="protein sequence ID" value="CCZ84475"/>
        <property type="gene ID" value="BN566_00374"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:inorganic phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011864">
        <property type="entry name" value="Phosphate_PstC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02138">
        <property type="entry name" value="phosphate_pstC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="59"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="107"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="255"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="66"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ84475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="282" mass="29912" checksum="1EE2BF221F4E2990" modified="2013-07-24" version="1">MKNVKENIMRFVFILAACVSIAAVALICIFLFANGIPAIAKIGPLKFLGGTTWNPNQEIFGIFPMIVGSIYATAIAMIVGVPIGLLTSVFLARFCPEKLYKVLKPAVSLLAGIPSIVYGFFGLVVMVPIMQNLTGVSGKNVLTAGILLGFMILPTIIEVSEAQIRAVPNSYYEGSLALGATHERSVYFAMLPAAKSGIMAGVILGIGRAIGETMAVVMIAGNQAVLPKSLVSGVRTMTANIVLEMGYATDLHREALIATGVVLFVFILIINVCFSLVKKGRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>R5V3V9</accession>
    <name>R5V3V9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Helix-turn-helix AraC domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00594</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000008">
        <property type="protein sequence ID" value="CCZ82789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00594"/>
    <dbReference type="EnsemblBacteria" id="CCZ82789">
        <property type="protein sequence ID" value="CCZ82789"/>
        <property type="gene ID" value="BN566_00594"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="1">
        <location>
            <begin position="169"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="287" mass="33360" checksum="CBFE0C7C0BACA09D" modified="2013-07-24" version="1">MKNAYPSYQEFHMHQPFFSFDVKQYEEQVQTTPYTARFYQFSTHKIIDRSQNQDLFMVCIPDGCVDVVFIQHDQQFKLELIGSPLARKPLIVYPDAAYFGIRMKPGMFFPDVPLSIREVTNTETFVPQLSPAVCSLMERLFATEQLSERVAIVQSELAQTEPESCTVPETVQDMLYLMASSNGELEISEMAETLCYSERHLNRMFSKALGYAPKMFARITRFQAALHTMMQQSHEEKSISDYITMFHYADQAHFQRECKEFTGLTPKHFVDYYLHTVCPQQEGKKPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5V440</accession>
    <name>R5V440_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00686</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000022">
        <property type="protein sequence ID" value="CCZ82884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82884">
        <property type="protein sequence ID" value="CCZ82884"/>
        <property type="gene ID" value="BN566_00686"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="292" mass="32515" checksum="DDFDB1CDFA7B6FEC" modified="2013-07-24" version="1">MSVISLLYFLLIVALAVLSFFYTVEVPQKAACCVVLIAISGGALAAMLYSRKQVLTKICSVLLLPFLLPSVLLCFGEWEVLVPLVVTALLAFFLCGCTETIKTVFGTIFLLLYVLGSLAYFLGSSLFSTSMASTVVDSGISPSRKYRYEVINTEDSSNGSTAVTVEPNNYDVNLGVLEFRAEGYERTVSLERPMQEETVVRWETQTRQQITEELLTISEDVTLQLPASDRKALGYDENTTIYLRNLTDQDLERLGVPQNNDVMYVNDQVCFRSYIAILEDYFSPSKHAISAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5V4C3</accession>
    <name>R5V4C3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00759</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000032">
        <property type="protein sequence ID" value="CCZ82959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82959">
        <property type="protein sequence ID" value="CCZ82959"/>
        <property type="gene ID" value="BN566_00759"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ82959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="40" mass="4680" checksum="72E895FA01756DD9" modified="2013-07-24" version="1">MEFEKPQMVILTAETKEEVSYSPERADDRGYEGFGNCCYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5V4Q6</accession>
    <name>R5V4Q6_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">YcxB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00867</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000051">
        <property type="protein sequence ID" value="CCZ83079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83079">
        <property type="protein sequence ID" value="CCZ83079"/>
        <property type="gene ID" value="BN566_00867"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025588">
        <property type="entry name" value="YcxB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14317">
        <property type="entry name" value="YcxB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="YcxB" evidence="2">
        <location>
            <begin position="112"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="179" mass="20630" checksum="2C45C8536D522DAD" modified="2013-07-24" version="1">MILENKTEVTVPAVMAMQKAMNRQGQLARYLIAGLFLLGAIWQIGYLMILGTWIQSYFYGILFCALVAGFLLIMPIWSNKKVLKQVTQQLCNSSAPLINHYYFQPQMLKIRDEGIPDGRDLQLPYSDITRFLQDDTYFFLYVKSTNSTYILKRNAFQPNDDVQVMACLQEKLAHCKGIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>R5V4T3</accession>
    <name>R5V4T3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SsrA-binding protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Small protein B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">smpB</name>
        <name evidence="4" type="ORF">BN566_00901</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Required for rescue of stalled ribosomes mediated by trans-translation. Binds to transfer-messenger RNA (tmRNA), required for stable association of tmRNA with ribosomes. tmRNA and SmpB together mimic tRNA shape, replacing the anticodon stem-loop with SmpB. tmRNA is encoded by the ssrA gene; the 2 termini fold to resemble tRNA(Ala) and it encodes a 'tag peptide', a short internal open reading frame. During trans-translation Ala-aminoacylated tmRNA acts like a tRNA, entering the A-site of stalled ribosomes, displacing the stalled mRNA. The ribosome then switches to translate the ORF on the tmRNA; the nascent peptide is terminated with the 'tag peptide' encoded by the tmRNA and targeted for degradation. The ribosome is freed to recommence translation, which seems to be the essential function of trans-translation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="3">The tmRNA-SmpB complex associates with stalled 70S ribosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SmpB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000059">
        <property type="protein sequence ID" value="CCZ83114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00901"/>
    <dbReference type="EnsemblBacteria" id="CCZ83114">
        <property type="protein sequence ID" value="CCZ83114"/>
        <property type="gene ID" value="BN566_00901"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070929">
        <property type="term" value="P:trans-translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09294">
        <property type="entry name" value="SmpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00023">
        <property type="entry name" value="SmpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023620">
        <property type="entry name" value="SmpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000037">
        <property type="entry name" value="SsrA-bd_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020081">
        <property type="entry name" value="SsrA-bd_prot_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30308">
        <property type="entry name" value="PTHR30308"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01668">
        <property type="entry name" value="SmpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74982">
        <property type="entry name" value="SSF74982"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00086">
        <property type="entry name" value="smpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01317">
        <property type="entry name" value="SSRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="156" mass="17962" checksum="3E52D9F128A4F32C" modified="2013-07-24" version="1">MAQKQTRSKQIADNRAVRHSYFVLEDFEAGIALQGNEVKSIRQGNVNLKDSWCDIKDGTLVLKGMHISPYEKDGLARTDPRRERQLLMHKREILRLFGKIKQDGLTLIPLNLHWSGSNVKVQIGLCKGKKLYDKRQSAAEQAAKRQIDRTMKSQYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>R5V4X1</accession>
    <name>R5V4X1_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA modification GTPase MnmE</fullName>
            <ecNumber evidence="6">3.6.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">mnmE</name>
        <name evidence="6" type="synonym">trmE</name>
        <name evidence="9" type="ORF">BN566_00942</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="6">Binds 1 potassium ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer. Heterotetramer of two MnmE and two MnmG subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. TrmE GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.-.-" evidence="6"/>
    <dbReference type="EMBL" id="CBAR010000069">
        <property type="protein sequence ID" value="CCZ83159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00942"/>
    <dbReference type="EnsemblBacteria" id="CCZ83159">
        <property type="protein sequence ID" value="CCZ83159"/>
        <property type="gene ID" value="BN566_00942"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04164">
        <property type="entry name" value="trmE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00379">
        <property type="entry name" value="GTPase_MnmE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031168">
        <property type="entry name" value="G_TrmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018948">
        <property type="entry name" value="GTP-bd_TrmE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004520">
        <property type="entry name" value="GTPase_MnmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027368">
        <property type="entry name" value="MnmE_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025867">
        <property type="entry name" value="MnmE_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027266">
        <property type="entry name" value="TrmE/GcvT_dom1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12631">
        <property type="entry name" value="MnmE_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10396">
        <property type="entry name" value="TrmE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00450">
        <property type="entry name" value="mnmE_trmE_thdF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51709">
        <property type="entry name" value="G_TRME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 6" id="KW-0630">Potassium</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6 7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TrmE-type G" evidence="8">
        <location>
            <begin position="214"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>(6S)-5-formyl-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57457"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>(6S)-5-formyl-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57457"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>(6S)-5-formyl-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57457"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="224"/>
            <end position="229"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="243"/>
            <end position="249"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="268"/>
            <end position="271"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="449"/>
        </location>
        <ligand>
            <name>(6S)-5-formyl-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57457"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ83159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="449" mass="48207" checksum="125266FB86A09C5F" modified="2013-07-24" version="1">METIAAIATPHAAGGISVIRISGDQAFAVADRIFHSDFGKIPSKMAGYTCAYGTLANLDDGILTAFRAPHSYTGEDVVELSCHGGIYLTQQILDAVLHAGAVPAQPGEFTKRAFCNGKLSLTQAEAVMDVIAASGSRELQFANAMREGAMVRRVHAVQEQLVTVLSGLAAWADYPEEDLPEVAPEALQTSLQQIQQDLKQLLQTKDYGRILKSGVLTVIAGKPNVGKSTLFNLLSGCERSIVTEIAGTTRDVVEECVRLGEVTLRLSDTAGLRETEDRIEQIGVRIAKEKLEQADLILAVFDSRQPLTEEDWALLEQVKSRPVIVILNKSDAGTAQITPEQLPDFPYCITMSAKAGSGLTELEAAVKGLFDRGDVTPELGILANARQTACAETALQMVTDALQALQSGELLDAVTVLLDEAAQALMTLTGERVTEAVVEEVFRHFCVGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5V4X9</accession>
    <name>R5V4X9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00952</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000069">
        <property type="protein sequence ID" value="CCZ83169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83169">
        <property type="protein sequence ID" value="CCZ83169"/>
        <property type="gene ID" value="BN566_00952"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="168" mass="17534" checksum="ACA72B5172030815" modified="2013-07-24" version="1">MQWKPMKEYKAPAYLAKAATLLAATTLLNGCGNLPVTPVDPPQSGTTATTAQTTTALSGEASIPQETTISTPPVTTTTTEAMFEGALTTPQPTTLDGDIIIPQETTTILERTFPVSQSTTTTTTEPMLAGTLTTPQPTTTMTTEPMFEGALTTPQPTTTTTTAAPEQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>R5V4Y8</accession>
    <name>R5V4Y8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RNA polymerase sigma factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN566_00962</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the sigma-70 factor family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000069">
        <property type="protein sequence ID" value="CCZ83179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00962"/>
    <dbReference type="EnsemblBacteria" id="CCZ83179">
        <property type="protein sequence ID" value="CCZ83179"/>
        <property type="gene ID" value="BN566_00962"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030435">
        <property type="term" value="P:sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000943">
        <property type="entry name" value="RNA_pol_sigma70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007630">
        <property type="entry name" value="RNA_pol_sigma70_r4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04545">
        <property type="entry name" value="Sigma70_r4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000770">
        <property type="entry name" value="RNA_pol_sigma-SigE/K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00046">
        <property type="entry name" value="SIGMA70FCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00715">
        <property type="entry name" value="SIGMA70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00716">
        <property type="entry name" value="SIGMA70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="3" id="KW-0749">Sporulation</keyword>
    <keyword evidence="7 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="9">
        <location>
            <begin position="199"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ83179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="234" mass="26491" checksum="2545956A9EAF8443" modified="2013-07-24" version="1">MKSLLQFLRQLQFWLCSGQMHYIHGADTLPPPLSAPEERAMLEALANGNTDAREQLIVHNLRLVVYIAKKFESTGIGLEDLISIGTIGLIKAVNTFCPTKKIKLATYASRCIENEILMFLRKATQYRGEVSIDEPLNIDWDGNELLLSDILGTDADVVSRNIEQESERELLRSAVSELGTREREIMELRFGLVDGKEKTQKEVAQAIGISQSYISRLEKKIIRKLRGTLQQFNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5V4Z5</accession>
    <name>R5V4Z5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00972</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000070">
        <property type="protein sequence ID" value="CCZ83189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00972"/>
    <dbReference type="EnsemblBacteria" id="CCZ83189">
        <property type="protein sequence ID" value="CCZ83189"/>
        <property type="gene ID" value="BN566_00972"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="152"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="193"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="242"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="274" mass="30271" checksum="AB27D5AAA2E89645" modified="2013-07-24" version="1">MGNVLKYEIKKSRFMMLIVGIIIGGLEIAYLIGFFGFKFAQNEKLNDIFTSIWALSTVLLIVAACCSMLAGALYSVLLYNSDIRKKSGYMLFLTPTPTWQWILAKILMGLLATAVLVVVFAVLGIADILLFIPELDDTSFIGQLLIPLRDMFFDNIGTMVLFLLNMILQIVVMITTIYLAISMSKTLLGNKKIGTFVAFVFWIALEILQSAISSLFTLLSNGLFSISIDMSENGLVYNLDTAVPFLLAAMALNLVFAGVCYGVTVHLTDKKMSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5V556</accession>
    <name>R5V556_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">4Fe-4S ferredoxin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_01040</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01040"/>
    <dbReference type="EnsemblBacteria" id="CCZ83259">
        <property type="protein sequence ID" value="CCZ83259"/>
        <property type="gene ID" value="BN566_01040"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12801">
        <property type="entry name" value="Fer4_5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="51"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="115"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="182"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="264"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="199"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ83259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="283" mass="31197" checksum="CAD22563295DBBA5" modified="2013-07-24" version="1">MKKKTIPAKKLHAYIRAAIQVVFFICFPSAFTTAFSAIKSIFTQIGSQAPIAWSSFLTVCLALCAFTIVFGRFFCGYACAFGSLGDAVRSLYVFICKKCKKKPITWKPKKLPLHFLKYGVLLVVGCLCFTGVYSDLKGWNPWDVFSMLRAGNIHLGGYWLGILLFAGILVGMALCERFFCRFLCPMGAVFSLLPMLPIFSVRRKQEQCAKGCSACTRCCPADLTLPEAGSWSVNGECFQCQKCVDICPKKNAHSGIGKLRGNEIWFTILRAVVLGVVLVLAGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>R5V594</accession>
    <name>R5V594_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">S-ribosylhomocysteine lyase</fullName>
            <ecNumber evidence="5">4.4.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">AI-2 synthesis protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Autoinducer-2 production protein LuxS</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BN566_01080</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Involved in the synthesis of autoinducer 2 (AI-2) which is secreted by bacteria and is used to communicate both the cell density and the metabolic potential of the environment. The regulation of gene expression in response to changes in cell density is called quorum sensing. Catalyzes the transformation of S-ribosylhomocysteine (RHC) to homocysteine (HC) and 4,5-dihydroxy-2,3-pentadione (DPD).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-(5-deoxy-D-ribos-5-yl)-L-homocysteine = (S)-4,5-dihydroxypentane-2,3-dione + L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:17753"/>
            <dbReference type="ChEBI" id="CHEBI:29484"/>
            <dbReference type="ChEBI" id="CHEBI:58195"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
            <dbReference type="EC" id="4.4.1.21"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the LuxS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.21" evidence="5"/>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01080"/>
    <dbReference type="EnsemblBacteria" id="CCZ83299">
        <property type="protein sequence ID" value="CCZ83299"/>
        <property type="gene ID" value="BN566_01080"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043768">
        <property type="term" value="F:S-ribosylhomocysteine lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009372">
        <property type="term" value="P:quorum sensing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037005">
        <property type="entry name" value="LuxS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003815">
        <property type="entry name" value="S-ribosylhomocysteinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35799">
        <property type="entry name" value="PTHR35799"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02664">
        <property type="entry name" value="LuxS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01487">
        <property type="entry name" value="LUXSPROTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="SSF63411"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0071">Autoinducer synthesis</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0673">Quorum sensing</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00015130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CCZ83299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="155" mass="17849" checksum="F1E199F0B2F27187" modified="2013-07-24" version="1">MKKIASFTINHDSLTRGMYVSRVDGDVVTYDIRMKTPNNPENDYLEDNALHTFEHLFATYVRNTPYSDEVVYVGPMGCRTGFYFLTRDTLPKETAIRLVQETISYVLQFEGEIPGSKRRECGNYLAHDLAGAKKIAQEIQPILKDWTPEQMTYPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>R5V5M9</accession>
    <name>R5V5M9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA gyrase subunit B</fullName>
            <ecNumber evidence="10">5.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">gyrB</name>
        <name evidence="12" type="ORF">BN566_01208</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterotetramer, composed of two GyrA and two GyrB chains. In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="10">Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the type II topoisomerase GyrB family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the type II topoisomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="10"/>
    <dbReference type="EMBL" id="CBAR010000104">
        <property type="protein sequence ID" value="CCZ83434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01208"/>
    <dbReference type="EnsemblBacteria" id="CCZ83434">
        <property type="protein sequence ID" value="CCZ83434"/>
        <property type="gene ID" value="BN566_01208"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03366">
        <property type="entry name" value="TOPRIM_TopoIIA_GyrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.670">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01898">
        <property type="entry name" value="GyrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002288">
        <property type="entry name" value="DNA_gyrase_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011557">
        <property type="entry name" value="GyrB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001241">
        <property type="entry name" value="Topo_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013760">
        <property type="entry name" value="Topo_IIA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013759">
        <property type="entry name" value="Topo_IIA_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013506">
        <property type="entry name" value="Topo_IIA_bsu_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018522">
        <property type="entry name" value="TopoIIA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034160">
        <property type="entry name" value="TOPRIM_GyrB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00204">
        <property type="entry name" value="DNA_gyraseB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00986">
        <property type="entry name" value="DNA_gyraseB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00433">
        <property type="entry name" value="TOP2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56719">
        <property type="entry name" value="SSF56719"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01059">
        <property type="entry name" value="gyrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00177">
        <property type="entry name" value="TOPOISOMERASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Toprim" evidence="11">
        <location>
            <begin position="427"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CCZ83434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="646" mass="72081" checksum="C05755AD1675C1B6" modified="2013-07-24" version="1">MTEERKEQDLQYDANQIQVLEGLEAVRKRPGMYIGSTGAKGLHHLVYEIVDNAIDEALAGYCTEIVVELLPDDVVRVADNGRGIPVGIHPKEGISAATVVYTILHAGGKFGGGGYKVSGGLHGVGASVVNALSEWLELTVRNGEHIYFQRFERGNYSKPLEQIGDTTETGTEVRFKADGEIFEHTVYDYEVLLKRLREQAFLNAGIRIVFRDLRNADAPREEILHYEGGIRQFVEHIHKTRGLTSLSGDVIYISGMEGDAMAEIALQYNDSYNELVLSFANNIHTVDGGTHEVGFKNALTKVMNEYGKKFNLLKDNTRLLGDDVREGLTAIISVKLTECEFEGQTKGRLGNPAVKPFIEKLVTEKLMNFLEENPAVARAIFDKSISAQKAREAAKKARDLTRRKSAMNNVSLPGKLADCSERGVDGTEIYIVEGDSAGGSAKEGRDRRYQAILPLWGKMLNVEKARIDKVYGNEKLMPIVTALGTSIGEDFDLSKLRYGKVIIMADADVDGSHIRTLLLTFFFRFMRPLITNGNIYIAQPPLFKVSKGKKFRYAFDEAERDAYIAEFAQGDSNTKINVQRYKGLGEMSSEQLWETTMNPETRSMLQVSLEDAELADEIFTILMGDQVTPRKQFIETNARYVQNLDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5V5N4</accession>
    <name>R5V5N4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">S4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01213</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TlyA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000105">
        <property type="protein sequence ID" value="CCZ83439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01213"/>
    <dbReference type="EnsemblBacteria" id="CCZ83439">
        <property type="protein sequence ID" value="CCZ83439"/>
        <property type="gene ID" value="BN566_01213"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004538">
        <property type="entry name" value="Haemolysin_A/TlyA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32319:SF0">
        <property type="entry name" value="PTHR32319:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005578">
        <property type="entry name" value="TlyA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00478">
        <property type="entry name" value="tly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S4" evidence="2">
        <location>
            <begin position="2"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="262" mass="28709" checksum="4BFDC62416FBF7F7" modified="2013-07-24" version="1">MTRLDVLLLQRGLVGSRQRAKNLITNGQIQVDGVVCTKPAKLTEETAALTLLGEDLRYVGRGGLKLEWILEQQNLSLEQATCMDIGASTGGFTDCMLQHGAKKVYAIDVGHGQLADSLRQDERVVCMEGTDIRTLPQTAIAEPIDFISVDVSFISLERILPDICRFLSDTGRAVLLIKPQFEAGRSDVGKNGIVRSKNVHVRVLRTVCMALEQQGLHIQYLCPSPIRGGSGNIEYLVQVQRGNVSPIPYAPEAVAEQAWSQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5V650</accession>
    <name>R5V650_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative recombinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00134</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000135">
        <property type="protein sequence ID" value="CCZ83589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00134"/>
    <dbReference type="EnsemblBacteria" id="CCZ83589">
        <property type="protein sequence ID" value="CCZ83589"/>
        <property type="gene ID" value="BN566_00134"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038109">
        <property type="entry name" value="DNA_bind_recomb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011109">
        <property type="entry name" value="DNA_bind_recombinase_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51737">
        <property type="entry name" value="RECOMBINASE_DNA_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Recombinase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="134" mass="15306" checksum="FCFCF897BDF332AC" modified="2013-07-24" version="1">MYGYRIESGKAVIDENEACILNTMFHNYLNGMSLTEAAETAGLHNCHSQIKKMLQREIYLGDQYYPPIIDKALFALVQTEIEKRSAAHCQRGKKRCIEPSILHDFCMDIPSKQFENPAEQAEYLYSLIGVKENA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5V6E9</accession>
    <name>R5V6E9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyltransferase group 2 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01440</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000154">
        <property type="protein sequence ID" value="CCZ83704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01440"/>
    <dbReference type="EnsemblBacteria" id="CCZ83704">
        <property type="protein sequence ID" value="CCZ83704"/>
        <property type="gene ID" value="BN566_01440"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="225"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="268"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_trans_2-like" evidence="2">
        <location>
            <begin position="5"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="328" mass="37686" checksum="1967DF6BD31ADBB0" modified="2013-07-24" version="1">MKLVSIVVPCYNEEKVLPLFYAEITKVMSQMQEKYPELDFELLFINDGSKDGTLQEFRKMATMDHRVRYVSFSRNFGKEAGMYAGLQHAKGDYVVIMDADLQHPPAFLPQMYEYVKDGEYDCATTRRVSRKGESKIRSWFSRKFYKIMNHISQTEIVDGAQDFRFMTRQMVNAILEMKEYNRFSKGIFSWVGFRTRYIPYENVERAAGTTSWSFKSLFKYSLEGIFAFSTAPLALASLLGIVSCLLAFIMILVVVIKYLAFGDPVQGYATTICVILLMGGLQLFCTGIVGQYLSKTYLETKNRPIYLVQETEEAVQQPDSKANPNRQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5V6V6</accession>
    <name>R5V6V6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Membrane proteins related to metalloendopeptidases</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01574</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000166">
        <property type="protein sequence ID" value="CCZ83839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01574"/>
    <dbReference type="EnsemblBacteria" id="CCZ83839">
        <property type="protein sequence ID" value="CCZ83839"/>
        <property type="gene ID" value="BN566_01574"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011055">
        <property type="entry name" value="Dup_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016047">
        <property type="entry name" value="Peptidase_M23"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01551">
        <property type="entry name" value="Peptidase_M23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51261">
        <property type="entry name" value="SSF51261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M23" evidence="3">
        <location>
            <begin position="150"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="255" mass="27885" checksum="C6C9B90D52D45231" modified="2013-07-24" version="1">MKKGTMKEKQTSKYKGFYTAIGISVLMIGAACVYAYQVTMETNQTLENELSQTAEETQPTTNFIAEIYDAVTTTQPEYDAVAKVKTDVPVETTTEAVEETEPQEEPESEPEETEEVIYSHMLMIPVDGEVAQPFSDGELVKSLTTGTWQTHNGVDILAEQGVPVRAMDNGTVVEVVNDPLWGICVSVDHENGIVSRYCNLSPNVGVQEGDTVESGQTLGTVGDSADIESKMESHLHFEVTKGGDYIDPYAYVQHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5V787</accession>
    <name>R5V787_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mannose-6-phosphate isomerase</fullName>
            <ecNumber evidence="3">5.3.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_01688</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-mannose 6-phosphate = D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:12356"/>
            <dbReference type="ChEBI" id="CHEBI:58735"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="EC" id="5.3.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="4">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mannose-6-phosphate isomerase type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.8" evidence="3"/>
    <dbReference type="EMBL" id="CBAR010000176">
        <property type="protein sequence ID" value="CCZ83959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01688"/>
    <dbReference type="EnsemblBacteria" id="CCZ83959">
        <property type="protein sequence ID" value="CCZ83959"/>
        <property type="gene ID" value="BN566_01688"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004476">
        <property type="term" value="F:mannose-6-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001250">
        <property type="entry name" value="Man6P_Isoase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014628">
        <property type="entry name" value="Man6P_isomerase_Firm_short"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01238">
        <property type="entry name" value="PMI_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036894">
        <property type="entry name" value="PMI_Firm_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="active site" evidence="5">
        <location>
            <position position="197"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR036894-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR036894-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ83959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="325" mass="35950" checksum="24590A0BE51B8C75" modified="2013-07-24" version="1">MTKPEPFLLQPAGKDYLWGGNRLHTLFGKQIPLHPLAETWECSTHPDGCSIVASGVDKGKSLSEVLQQHPEFLGAKTSATGDLPVLVKLIDAAKDLSVQVHPDDAYAQQYEHEPNGKTEMWYILEAEPGASLIYGFSKLMQPEQLDTCLKENTILSYLQKIPVKKGDVFLIPAGTVHAIGAGIVLAEIQQSSNLTYRLYDYNRTDATGKKRPLHIKKAKAVIQFSANQPPQTKQPMQQKDGYRVQCLCKCPYFCTTLLTIQTACDWTDYQPDSDTFQVLLCISGSGTIQTKENALSFQKGDCIFLPAHCSMERLTGTAELLQITG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7H0</accession>
    <name>R5V7H0_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">WYL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01750</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000180">
        <property type="protein sequence ID" value="CCZ84024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01750"/>
    <dbReference type="EnsemblBacteria" id="CCZ84024">
        <property type="protein sequence ID" value="CCZ84024"/>
        <property type="gene ID" value="BN566_01750"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026881">
        <property type="entry name" value="WYL_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13280">
        <property type="entry name" value="WYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WYL" evidence="1">
        <location>
            <begin position="139"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="327" mass="38907" checksum="D3EB10474D243231" modified="2013-07-24" version="1">MEHERIIHILEYLRRNTDETRTVSIKQIQSHLDRNYNMQSVSPLTIRRDIERLTTMGYPITIQRGAHNTYLYQMETEGFTFNEIRFLVDSVSINKYLSVDQKQHLIKKFERLCSESDVRKLISRIQTNDLTPPGLNLLENLEQIHLIIANRQKIDFEYGKQEKDGMKYYYKKRNMIPCQVVFFEDRFYLKCIHAETKEPRTYRIDRMKQIQPGETVSEMPKLPKPKGAILDIFDPKYYETVTLRIKSFLTGEMKEKFGLYLHIIPGETTSEETVIRVTVGISDSFFRWLMRYGSYVELISPESLRKQFQEQLQEVAALYQKKEEESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7N1</accession>
    <name>R5V7N1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Predicted divalent heavy-metal cations transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_01835</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000187">
        <property type="protein sequence ID" value="CCZ84109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01835"/>
    <dbReference type="EnsemblBacteria" id="CCZ84109">
        <property type="protein sequence ID" value="CCZ84109"/>
        <property type="gene ID" value="BN566_01835"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046873">
        <property type="term" value="F:metal ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="67"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="111"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="141"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="180"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="208"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="237"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ84109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="260" mass="27340" checksum="B32A7968C8B103E6" modified="2013-07-24" version="1">MTQVVQGILIPFLGTTLGAACALFLKNNLPTSVQRILTGFAAGIMTAASVWSLLIPALEQESDRGRLAFLPAAIGFLVGIAFLLLLDRLIPHLHMNSKEAEGIPARLKKTTMLVFAVTLHNIPEGMAVGVVYAGVLYGHQASITAAGALALSLGIAIQNFPEGAIISMPLRAEGMRKSKAFLYGTLSGVVEPLGALLTILASGFFIPLMPYLLSFAAGAMLYVVVEELIPEMSEGKHSNVGTISFAVGFVAMMILDTALG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7P5</accession>
    <name>R5V7P5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Glutamate synthase large subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01850</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000189">
        <property type="protein sequence ID" value="CCZ84124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01850"/>
    <dbReference type="EnsemblBacteria" id="CCZ84124">
        <property type="protein sequence ID" value="CCZ84124"/>
        <property type="gene ID" value="BN566_01850"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036485">
        <property type="entry name" value="Glu_synth_asu_C_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69336">
        <property type="entry name" value="SSF69336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="106" mass="12170" checksum="91FDE64B3C617C84" modified="2013-07-24" version="1">MSGGIAYVLDEESDLYTKLNKALVGFSKVTHPEDVAQLKELITEHVARTHSVRGQEILDQFDAYLPKFKKIVPHDYAKMLRSIRQFMDQGLPQEEAKIEAFYVNTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7R9</accession>
    <name>R5V7R9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01879</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000193">
        <property type="protein sequence ID" value="CCZ84154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84154">
        <property type="protein sequence ID" value="CCZ84154"/>
        <property type="gene ID" value="BN566_01879"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="65" mass="7742" checksum="AEFB5A7EE0FBC4FC" modified="2013-07-24" version="1">MKQRYQIITIYEEDHGCEGIPEGEEPMDQVLLRTEAGEEHWTRIADAVLLNQNWNEGDWILWEIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5V944</accession>
    <name>R5V944_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_00477</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the autoinducer-2 exporter (AI-2E) (TC 2.A.86) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000257">
        <property type="protein sequence ID" value="CCZ84624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00477"/>
    <dbReference type="EnsemblBacteria" id="CCZ84624">
        <property type="protein sequence ID" value="CCZ84624"/>
        <property type="gene ID" value="BN566_00477"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002549">
        <property type="entry name" value="AI-2E-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21716">
        <property type="entry name" value="PTHR21716"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01594">
        <property type="entry name" value="AI-2E_transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="31"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="75"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="162"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="232"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="259"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="291"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="322"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ84624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="384" mass="42640" checksum="B8F605B3684DA855" modified="2013-07-24" version="1">MKQWQQKVFGIVLFIAAIFLILANFEESSYAFSFLFELIQPVIVAGVLAFFLHIPMNGFRNFLQKHAKRPHKERFWNTISLVLTLLSVCIVLAAVCMLMIPALIQSIMDIYNKVQHNLPIWLNQLAAYGIDTAMLEEWLKNAEQGQLLQKLIVGVGNWVPTIFSAAASTASVVVDGVVGFILAIYLLMERHTIGRQLNKVLEANLQHTTCQKIRDTGNLICQIFRSFLCGQCLEAVILGTLMFIAFAICGISYAGLIGVLTAVCSFIPFVGAFLACCTGALLNLMIDPWKALLCILVYLVVQFIEGHFIYPKVVGNSVGLSSFWVLVAVLVGQNLFGVFGMILFIPLTSVIYTLLKNDTNRKLKKKAELAQETVGTDSEPHQTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5VGY3</accession>
    <name>R5VGY3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00636</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000012">
        <property type="protein sequence ID" value="CCZ82831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00636"/>
    <dbReference type="EnsemblBacteria" id="CCZ82831">
        <property type="protein sequence ID" value="CCZ82831"/>
        <property type="gene ID" value="BN566_00636"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003772">
        <property type="entry name" value="YceD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02620">
        <property type="entry name" value="YceD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ82831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="166" mass="18411" checksum="573831A99B5FBFA8" modified="2013-07-24" version="1">MKLQLREIFQVTGMHLPVSYSIPLSELEDVHGYTFAEPIAVEGEFVNRAGIVTLNYQVSCRLDAVCDRCLKPFTPSFSYTFSHTVVPSLHSDNPAYDAYLVAENDKIDLNETAISDLLLMLPTKLLCREDCKGLCDICGCDRNVTDCDCLKEKGGANDGCTEEKGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5VH30</accession>
    <name>R5VH30_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative cobalt-precorrin-6A synthase [deacetylating]</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_00673</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000020">
        <property type="protein sequence ID" value="CCZ82871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00673"/>
    <dbReference type="EnsemblBacteria" id="CCZ82871">
        <property type="protein sequence ID" value="CCZ82871"/>
        <property type="gene ID" value="BN566_00673"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002748">
        <property type="entry name" value="CbiD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036074">
        <property type="entry name" value="CbiD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35863">
        <property type="entry name" value="PTHR35863"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01888">
        <property type="entry name" value="CbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111342">
        <property type="entry name" value="SSF111342"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00312">
        <property type="entry name" value="cbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ82871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="284" mass="30319" checksum="C6E08A3936F7AF17" modified="2013-07-24" version="1">MKIGTTDGAVTFQAGEGVGIVTKKGLRVPVGEPAINPVPRTMITEAVRSVIGTRSAVVIISVPNGEQIAERTFNGRLGIVGGISILGTTGVVRPMSEEAVRESLRLELSMCRAEYGTACALVTGYAGETYLKKRYEHAGKIVLCSNYLGYLLDCAEEFGIQTLLLAGVAGKLMKPAANIFYLHSHTAGGQREILCTHAALAGATTAQIQQLYACNTTTEMQELLAKMPFSDVVWQSIAEAALENCQKRTHGTMQIGLILLDRTGTVLAETEQVSQVGKAWCQDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5VHM7</accession>
    <name>R5VHM7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Chloride transporter ClC family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_00794</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000036">
        <property type="protein sequence ID" value="CCZ83001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83001">
        <property type="protein sequence ID" value="CCZ83001"/>
        <property type="gene ID" value="BN566_00794"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005247">
        <property type="term" value="F:voltage-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014743">
        <property type="entry name" value="Cl-channel_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="Cl-channel_volt-gated"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00654">
        <property type="entry name" value="Voltage_CLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81340">
        <property type="entry name" value="SSF81340"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="145"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="214"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="254"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="293"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="321"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="359"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ83001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="411" mass="43267" checksum="9B002DD56708153E" modified="2013-07-24" version="1">MHAIKEKLLPMGLLCLLAILTGAITGVITAGFGRILLGIGTLRTTHLLWFLPFLPLAGALMVWAYQKYGKESSQGMGLIFDAATGKADRIPLRLIPLLIGSTWLTHLCGGSAGREGVAVQLGATVAHWTGSHIPLFQRQKSLIRILTISGMAAGFSGLFHAPLAAVCFALEVLVVGKLEYRALLPAALSAITANIVSIALGLETFQVTLPETVVSPISISMLILGVAAGLTGGVFTFLLKQAKQAAAKWLPNPILRIAICGAGLAILLYLCGKGRYSGLGTNLITLSCNGDTVYWFDFLGKLGFTVLTLAIGFQGGEVTPLFAIGACLGAVLAPLLGIPSVVAVALCYAAVFGSASNTLLAPICIGAEVFGFSYFPYFAVVCTIARLCNGNHSMYGKQTTTPLFFWREDCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5VHT4</accession>
    <name>R5VHT4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00854</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000050">
        <property type="protein sequence ID" value="CCZ83066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00854"/>
    <dbReference type="EnsemblBacteria" id="CCZ83066">
        <property type="protein sequence ID" value="CCZ83066"/>
        <property type="gene ID" value="BN566_00854"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="130" mass="14176" checksum="99EB8C6342AED0E0" modified="2013-07-24" version="1">MQDQNHFFESEDHTMEFDTQDAQDAKIWSVLAYFGILFFLPLVAVPNSAYGKFHANQGLILLIFYVTLTVASWLVRAVLGIIPIIGGILSGLVQLAVSVCIIALIVFGMVQAAQGKAKTLPVIGKFHLIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5VI03</accession>
    <name>R5VI03_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00964</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000069">
        <property type="protein sequence ID" value="CCZ83181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83181">
        <property type="protein sequence ID" value="CCZ83181"/>
        <property type="gene ID" value="BN566_00964"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="597" mass="65388" checksum="EDEE07634E3DB64E" modified="2013-07-24" version="1">MNKNRYQQIMNRIQMPERCEQAILEQIAHNASPVPTRRTWKRNVIAGVAIAACISVIGMVSVSAIQGTNWIHQLFPNSSDDAATAQYASQQAGNISAFSADLDDLDAELIAAFADESNLYYAVHLTPKTENPEKYTDRFTISQTGTFWFNGETNTEFLQGSASETRPDGDGYLFTNKIHLDSGTWENHMTYQAEMQAILYEGEQYETLKQNFGTIGTISITIDLQNKPQTSVYTASGTWNNASGFLNVEQVVLQPLTLTIDGTADNQTNSAKQVSVILKDGSTVAGEYMTSGYRNHGIVTEDLTQVEQFLYLEKPIDPTAATAIQMDDVTIPLTAKADNTILDPASMVATISNFTTTGMDDFTVTPLGVLSDGTNLAMVLNLAPNQPDMLPEETMFIIDVCSLSIDNKPLSELLSSESLNTVYAYSEQQTDGTYTFTCKIQMDAFLNQQADVAFALKTSDDSTLGTVAFHLDNIQTIPSVTYTADGIWKHYVEIQKTYQVFHIQQVTCYPSHIKLAGKMDRDLVTQPESVSVILADGTMVSAVDLQSGKGGLSAGYGVSHNLDGTGWLEFDLPQPIDPNAVTYLKVDQTIIPLTAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VI22</accession>
    <name>R5VI22_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00989</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000070">
        <property type="protein sequence ID" value="CCZ83206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83206">
        <property type="protein sequence ID" value="CCZ83206"/>
        <property type="gene ID" value="BN566_00989"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="304" mass="34353" checksum="AE6398662E290200" modified="2013-07-24" version="1">MVDRCCKFSCRQVHIFWFQSTDFNNICLILVHEIAVVSVFILTESNAIPCRLFAVQSIYNIPAAPAKQLPLIFEQSCIFFHIECLCSRKRLDFQIDAHFQVVSTVEFLLYERGMLPFFFQAEIDISIQIRINAHFNRNADFSIFFKSCKGKGQLAFQTGFHTNSSAGIEIVGVTVCTTQFIFISLYFSAGNASHANLCGIQLGKVTGNASCQIQPQGIIQIQTNCENWNKRNDTEFSADNRFIADVNKSSVRIDDIAVSLEHFFRSHLTGDTFGNVQPFAALFPNGNIEIKCHLRIDICSIVKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>R5VIX8</accession>
    <name>R5VIX8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Chaperone protein DnaJ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">dnaJ</name>
        <name evidence="10" type="ORF">BN566_01240</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Binds 2 Zn(2+) ions per monomer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="6">The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DnaJ family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000111">
        <property type="protein sequence ID" value="CCZ83466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01240"/>
    <dbReference type="EnsemblBacteria" id="CCZ83466">
        <property type="protein sequence ID" value="CCZ83466"/>
        <property type="gene ID" value="BN566_01240"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01152">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012724">
        <property type="entry name" value="DnaJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00684">
        <property type="entry name" value="DnaJ_CXXCXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="SSF46565"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="SSF49493"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="SSF57938"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02349">
        <property type="entry name" value="DnaJ_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 6" id="KW-0235">DNA replication</keyword>
    <keyword evidence="2 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0346">Stress response</keyword>
    <keyword evidence="5 6 7" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 6 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="J" evidence="8">
        <location>
            <begin position="7"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="CR-type" evidence="9">
        <location>
            <begin position="145"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="repeat" description="CXXCXGXG motif" evidence="6">
        <location>
            <begin position="158"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="repeat" description="CXXCXGXG motif" evidence="6">
        <location>
            <begin position="175"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="repeat" description="CXXCXGXG motif" evidence="6">
        <location>
            <begin position="201"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="repeat" description="CXXCXGXG motif" evidence="6">
        <location>
            <begin position="215"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="7">
        <location>
            <begin position="145"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ83466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="387" mass="41756" checksum="A5263B441C2077AA" modified="2013-07-24" version="1">MAEEKRDYYEVLGLQKGASEEEIKKAFKTMARKYHPDLHPGDKEAEAKFKEVNEAYGVLSDADKRARYDQFGHAGVDPNFAGAGGGAGDFGGFGDMGDIFESIFGGFGGFGGGGGRSAAANANAPRRGGDIHSNVRISFMEACQGVKKTISFAHMDACPQCHGTGADSNTKKQVCPECNGRGSVKTNQRTPFGVISSTRVCSRCGGRGEIVTNPCSKCKGSGRIRSTANLELNIPAGINDGETIRISGKGDAGLNGGSAGDVYVTVEMMSHPLFEREGSDIYCDIPITYAQAVLGAEITVPTIDGDVKYTIPEGTQSGTKFRLRGKGVKLLRRDARGDQYVTVNVEVPKKLSKKQKELLQDFDNSLDEKNHSKRENFFDKLKQLRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJA7</accession>
    <name>R5VJA7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Extracellular solute-binding protein family 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01364</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000144">
        <property type="protein sequence ID" value="CCZ83601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01364"/>
    <dbReference type="EnsemblBacteria" id="CCZ83601">
        <property type="protein sequence ID" value="CCZ83601"/>
        <property type="gene ID" value="BN566_01364"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13416">
        <property type="entry name" value="SBP_bac_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004395409" evidence="1">
        <location>
            <begin position="28"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="434" mass="47535" checksum="B5BE1F9D930FFF29" modified="2013-07-24" version="1">MNTKKLLAAVTGVTMLAGMLTACGSSSDSSTASSSDPNTLTIWAWDDNFNVKAANLAKEYYQKEHPDVTVNVVSMAQDDIVQTLNTAFSSGTYKGLPNIVLIEDYRIQNYLNAYPGELRDLSNTVNPDDFMDYKLGVMTQDNKVYGVPFDSGVAALFYRTDYIKEAGYTTDDMQDLTWSEFIQIGKDVKEKTGKYMLTLDPSDLGQIRMMMQSAGAWYVKEDGKTVDIADNEVLKESVKTYQQIIEAGIAEQISGWDPFVGAFQKGDVASVPTGCWIAPTIAAAEDQSGKWAVAPLPRLDNVDSSLNTSNIGGGSWYVLDKVGNGELAENFLAETFASNVDLMNDLVSEINLVSTLKASADAENYKKPNEFFGGQEIFSDFSKWTEEIPTVNYGLYTYSIEDIMSEAVQSILQGADIDETLANTQVQAEAAVLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJJ7</accession>
    <name>R5VJJ7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phosphoribosylglycinamide formyltransferase</fullName>
            <ecNumber evidence="2">2.1.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">5'-phosphoribosylglycinamide transformylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">GAR transformylase</fullName>
            <shortName evidence="2">GART</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">purN</name>
        <name evidence="4" type="ORF">BN566_01447</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the transfer of a formyl group from 10-formyltetrahydrofolate to 5-phospho-ribosyl-glycinamide (GAR), producing 5-phospho-ribosyl-N-formylglycinamide (FGAR) and tetrahydrofolate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>(6S)-10-formyltetrahydrofolate + N(1)-(5-phospho-beta-D-ribosyl)glycinamide = (6S)-5,6,7,8-tetrahydrofolate + H(+) + N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide</text>
            <dbReference type="Rhea" id="RHEA:15053"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57454"/>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="EC" id="2.1.2.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GART family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.2" evidence="2"/>
    <dbReference type="EMBL" id="CBAR010000154">
        <property type="protein sequence ID" value="CCZ83711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01447"/>
    <dbReference type="EnsemblBacteria" id="CCZ83711">
        <property type="protein sequence ID" value="CCZ83711"/>
        <property type="gene ID" value="BN566_01447"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00126"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004644">
        <property type="term" value="F:phosphoribosylglycinamide formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd08645">
        <property type="entry name" value="FMT_core_GART"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01930">
        <property type="entry name" value="PurN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002376">
        <property type="entry name" value="Formyl_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004607">
        <property type="entry name" value="GART"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00551">
        <property type="entry name" value="Formyl_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="SSF53328"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00639">
        <property type="entry name" value="PurN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Formyl_trans_N" evidence="3">
        <location>
            <begin position="2"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="5'-phosphoribosylglycinamide binding" evidence="2">
        <location>
            <begin position="12"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>(6S)-10-formyltetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57454"/>
        </ligand>
    </feature>
    <feature type="site" description="Raises pKa of active site His" evidence="2">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="213" mass="23360" checksum="7CE75FEB8C148B93" modified="2013-07-24" version="1">MKNIIVLVSGGGTNLQALLEAEQAGAFPGGSITCVISSRGDAYALRRAEQFHVKSRVIAKKDYPDFSAYTDAMLLALKEEQADLIVLAGFLTILDERIIAAYPNRIINVHPSLIPAFCGEGFYGLRVHECALKRGVKYSGATVHFVNEIADGGPIILQKPVAVEEGDTPQTLQKRIMEEAEWKLLPEAVRLFCADKLEVHDNKVTLLEQPKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5VK00</accession>
    <name>R5VK00_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">50S ribosomal protein L1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rplA</name>
        <name evidence="10" type="ORF">BN566_01600</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Binds directly to 23S rRNA. The L1 stalk is quite mobile in the ribosome, and is involved in E site tRNA release.</text>
    </comment>
    <comment type="function">
        <text evidence="8">Protein L1 is also a translational repressor protein, it controls the translation of the L11 operon by binding to its mRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 9">Belongs to the universal ribosomal protein uL1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000169">
        <property type="protein sequence ID" value="CCZ83871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01600"/>
    <dbReference type="EnsemblBacteria" id="CCZ83871">
        <property type="protein sequence ID" value="CCZ83871"/>
        <property type="gene ID" value="BN566_01600"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00403">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01318_B">
        <property type="entry name" value="Ribosomal_L1_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005878">
        <property type="entry name" value="Ribosom_L1_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002143">
        <property type="entry name" value="Ribosomal_L1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023674">
        <property type="entry name" value="Ribosomal_L1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028364">
        <property type="entry name" value="Ribosomal_L1/biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016095">
        <property type="entry name" value="Ribosomal_L1_3-a/b-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023673">
        <property type="entry name" value="Ribosomal_L1_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00687">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002155">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56808">
        <property type="entry name" value="SSF56808"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01169">
        <property type="entry name" value="rplA_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01199">
        <property type="entry name" value="RIBOSOMAL_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0678">Repressor</keyword>
    <keyword evidence="7 8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6 8 9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 8" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="4 8" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="8" id="KW-0820">tRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ83871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="231" mass="24706" checksum="811D9554102AB23E" modified="2013-07-24" version="1">MKHGKKYVDSRKAVDASKQYASEEALGLVCENAKAKFDETVEAHIRLGVDSRHADQQVRGAVVLPNGTGKTVRVCVFCKEDKYEAAKAAGADFVGGQDLVDKITKENWMDFDVVIASPDMMGLVGRLGKILGPRGLMPNPKAGTVTPDVAKAINEAKAGKIEYRLDKTNIIHCPIGKASFGADKLVQNFEVLMDAIVKAKPSAAKGTYLKSCTVTSTMGVGVKVSTTKYGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5VKQ8</accession>
    <name>R5VKQ8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative flavoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01827</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000186">
        <property type="protein sequence ID" value="CCZ84101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01827"/>
    <dbReference type="EnsemblBacteria" id="CCZ84101">
        <property type="protein sequence ID" value="CCZ84101"/>
        <property type="gene ID" value="BN566_01827"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12682">
        <property type="entry name" value="Flavodoxin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="1">
        <location>
            <begin position="3"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="158" mass="16817" checksum="22E5A01F9ED6A49C" modified="2013-07-24" version="1">MKTLVAYFSASGITANVAKLLAEAAGADLFEIQPAVPYTNADLNWMDKKSRSTVEMQDKSSRPAIAAPCSNLADYDTIFVGFPIWWYVAPTIINTFLESGDFSGKKIVLFATSGGSGFGKTVENLKDSAPGATIVEGKLLNGTQSVAGLKSWVDSILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5VLB6</accession>
    <name>R5VLB6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Membrane-associated phospholipid phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00254</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000217">
        <property type="protein sequence ID" value="CCZ84341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00254"/>
    <dbReference type="EnsemblBacteria" id="CCZ84341">
        <property type="protein sequence ID" value="CCZ84341"/>
        <property type="gene ID" value="BN566_00254"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="P_Acid_Pase_2/haloperoxi_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="P_Acid_Pase_2/haloperoxidase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="SSF48317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="acidPPc" evidence="2">
        <location>
            <begin position="63"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="181" mass="20018" checksum="95E7C886B729B4B8" modified="2013-07-24" version="1">MQGLTTLDFQILHAIQRIFSCSVLDFLMPKITTLGNGGILWILLAIFLLILPKTRKNGIQLGVGCVGCGLIGNLFLKNVIARNRPCWIEDHTMLIAMPTDFSFPSGHTMVSFAAATVLWHWNRKVGIAAYLLAAVIAFSRLYLFVHFPSDVFAGAVIGILIGIAAIKGTDWMQKKFQKQKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5VLE6</accession>
    <name>R5VLE6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">PF06541 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_00278</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000221">
        <property type="protein sequence ID" value="CCZ84371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00278"/>
    <dbReference type="EnsemblBacteria" id="CCZ84371">
        <property type="protein sequence ID" value="CCZ84371"/>
        <property type="gene ID" value="BN566_00278"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010540">
        <property type="entry name" value="CmpB_TMEM229"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06541">
        <property type="entry name" value="ABC_trans_CmpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="137"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="173"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="240"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="281"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ84371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="340" mass="38537" checksum="7CE6E1ACE4AB407C" modified="2013-07-24" version="1">MSPVKTKTQTIAQPTELTAAPVATLLQHSQDGLDTLNEADQHLQTAYAELAQETLPLDAESRRRHESLREEITVLKNATENYKKCYNTASAMTDELQTRLHDTERSQKKGIVPKPPANATIDFAERKQDHFAQGLNFYKLFLICFSGSFAGVIVELLWCLLRNGYLESRSGLVYGPFNLLYGAGAIALTLALYRFRNRNSWISFLGGFLVGSAVEYACSWGQEMIFGSCSWDYSSMPFNLNGRICLLYSIFWGALGVLWIKTLYPWIAKGILHIPNRIGKILTWLLFAFILFDAVMTVGAVFRWSQRIDGIAATNSLQLFFDVHFPDVRMERIFANMKFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5VLL8</accession>
    <name>R5VLL8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00348</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000229">
        <property type="protein sequence ID" value="CCZ84441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84441">
        <property type="protein sequence ID" value="CCZ84441"/>
        <property type="gene ID" value="BN566_00348"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="254" mass="29214" checksum="40E9F4A1DF57DA71" modified="2013-07-24" version="1">MKCKFCEQELPEGAEKCPNCGIPVRSNQKESPPIGAAMTTYRTPEQVKDAEPIADIEPPEVRYERLERTSRRNRWIALCAILLVLILLVSGYFLFFCGYRATIRRYVKGRDSMSGTRYCEIVPDAFLSKMSSKYSMNRKEIRDCLDGYFNYAKEQMVDDLGSNIEFGYERDEETTYTDEETCSEYEQKIADTYSVTVDIDKVVLANVRIVTSGSNQNATDSLTLTLFKSGSKWYCMDAMEQVDYACQYDGYDLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5VLR5</accession>
    <name>R5VLR5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Helix-turn-helix domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00380</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000239">
        <property type="protein sequence ID" value="CCZ84481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84481">
        <property type="protein sequence ID" value="CCZ84481"/>
        <property type="gene ID" value="BN566_00380"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12844">
        <property type="entry name" value="HTH_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="17"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="83" mass="9573" checksum="51C2A20D62EF7A89" modified="2013-07-24" version="1">MARIIDQQKKNLIGERVRFYRIQRGYSQQQLADKLETLAIYICRGSISRIEDYSRTVTDLELVALAEVLQVSVLDLLPESLFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>R5VM04</accession>
    <name>R5VM04_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">50S ribosomal subunit assembly factor BipA</fullName>
            <ecNumber evidence="3">3.6.5.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">GTP-binding protein BipA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">bipA</name>
        <name evidence="5" type="ORF">BN566_00407</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">A 50S ribosomal subunit assembly protein with GTPase activity, required for 50S subunit assembly at low temperatures, may also play a role in translation. Binds GTP and analogs. Binds the 70S ribosome between the 30S and 50S subunits, in a similar position as ribosome-bound EF-G; it contacts a number of ribosomal proteins, both rRNAs and the A-site tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>GTP + H2O = GDP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="3">Binds to ribosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. BipA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.-" evidence="3"/>
    <dbReference type="EMBL" id="CBAR010000242">
        <property type="protein sequence ID" value="CCZ84546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00407"/>
    <dbReference type="EnsemblBacteria" id="CCZ84546">
        <property type="protein sequence ID" value="CCZ84546"/>
        <property type="gene ID" value="BN566_00407"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03710">
        <property type="entry name" value="BipA_TypA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00849">
        <property type="entry name" value="BipA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035651">
        <property type="entry name" value="BipA_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042116">
        <property type="entry name" value="TypA/BipA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006298">
        <property type="entry name" value="TypA_GTP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908:SF8">
        <property type="entry name" value="PTHR42908:SF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="SSF54980"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01394">
        <property type="entry name" value="TypA_BipA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="3" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Tr-type G" evidence="4">
        <location>
            <begin position="4"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="16"/>
            <end position="21"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="129"/>
            <end position="132"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ84546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="607" mass="67381" checksum="1086BF450ADB0ED5" modified="2013-07-24" version="1">MLREDLRNVAIIAHVDHGKTTLVDEMLKQGGVFRENQAVAERVMDSNALERERGITILAKNTSVRYGDIKINIIDTPGHADFGGEVERVLSMVDGALLLVDAAEGPMPQTRFVLQKALEMNLKIIVVVNKIDRPDARLDEIGDEVLELLMDLDASEEQLENTPFLYCSGRDGTASLDPHGNGTDLIPLFDTIVNYVNPPEGDPEAPLQMLISSIDYNEYVGRIGIGRIARGTIQVNQQVMIGNYHDPETASRNKVVSLLQIQGLQKVPAESATVGDIVWISGISNISIGDTVCAPDCFEPLPFTKISEPTVEMTFCVNDSPFAGQEGKFVTSRQLRDRLMRELLRDVSLRVTETDSTDAFRVAGRGEMHLSILIENMRREGYELGVSTPRVLYHEVDGKRYEPIEEVIIDVPEECVGSVMEKLGTRKAELQDMHPQGSRTRLTFLIPSRGLFGYKSEFLTDTKGEGIMNSMFHGYEPYKGEIPRRNTGSLISFETGDAVTYGLYNAQERGSLFIGAGVPVYEGMVVGASPKTEDLVVNVCKRKHLTNTRASGSDDALRLVPPRIMSLEDCLEFLADDELLEVTPKSLRIRKQILSNQLRAKQRGKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5VM35</accession>
    <name>R5VM35_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00090</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000252">
        <property type="protein sequence ID" value="CCZ84596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84596">
        <property type="protein sequence ID" value="CCZ84596"/>
        <property type="gene ID" value="BN566_00090"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023811">
        <property type="entry name" value="CHP04076"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04076">
        <property type="entry name" value="TIGR04076"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="106" mass="11868" checksum="5AB6A16875BA9C5F" modified="2013-07-24" version="1">MKKCRITALRRTEYPDLMEQYENPLETPCMVSIGQVWVTNGCEKPADFCESAWNTLSPFVMALAHGGENFYDGWMKNPKLVLLSCNDGFRPVSFLVEALEADGGEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5VM46</accession>
    <name>R5VM46_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN566_00464</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the membrane-bound acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000257">
        <property type="protein sequence ID" value="CCZ84611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00464"/>
    <dbReference type="EnsemblBacteria" id="CCZ84611">
        <property type="protein sequence ID" value="CCZ84611"/>
        <property type="gene ID" value="BN566_00464"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042121">
        <property type="term" value="P:alginic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024194">
        <property type="entry name" value="Ac/AlaTfrase_AlgI/DltB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028362">
        <property type="entry name" value="AlgI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500217">
        <property type="entry name" value="AlgI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016636">
        <property type="entry name" value="AlgI_DltB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="109"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="146"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="225"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="326"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="356"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="409"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="436"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR016636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ84611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="465" mass="54115" checksum="F87D735A42645A4B" modified="2013-07-24" version="1">MSFTTIEFMFRFLPIFLIVYYVVPTRYKNMILLIGSFVFYAWGQHFYLLLLMLSIVVNYTFGRLIGERRAQRKPLLILGLIYNFGLLVFFKYTNFFIENINALLTATHIQIPTISVVMPLGISFYTFQVVSYLVDVYRGEQRPVKNIINLGVYIAMFPQVTSGPIGLYSELEPTLLRRHCSVLNLESGLKTFIIGMGAKMLLANPMGTLWAGMSRYGYETLSCPYAWLGAFGYSFQLYFDFAGYSLMAMGLGQMLGLYVPRNFDHPYISGSMAEFWRRWHMTLGRWFKKYLYFPLGGSRCSFAKTIRNTFVVWAFTGLWHGASWNFVLWGLIFFVLLTIERLGLGKLLAKTKVLKHIYVIFLIPLTWVVFALPNMQDIATYFSRLFPFFADNTASFVNTKDVIRALHDYWYLLIASVVFCLPFPSRWYEKHKNSKLVILGLVLIYLMSVYKMQTQTSNPFLYYQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5VMB6</accession>
    <name>R5VMB6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA-binding helix-turn-helix protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00544</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000267">
        <property type="protein sequence ID" value="CCZ84691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00544"/>
    <dbReference type="EnsemblBacteria" id="CCZ84691">
        <property type="protein sequence ID" value="CCZ84691"/>
        <property type="gene ID" value="BN566_00544"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="15"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="79" mass="8821" checksum="438AE09A251440CE" modified="2013-07-24" version="1">MYHATLNPKIVGQVIAAFRKKKGVSQEVLSGLADIGRTHLSAIERGERKPTLETLYRISTALNVSMSDIVKEIERQIEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5VMC2</accession>
    <name>R5VMC2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Stage V sporulation protein AD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00549</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000268">
        <property type="protein sequence ID" value="CCZ84696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00549"/>
    <dbReference type="EnsemblBacteria" id="CCZ84696">
        <property type="protein sequence ID" value="CCZ84696"/>
        <property type="gene ID" value="BN566_00549"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010894">
        <property type="entry name" value="SpoVAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038369">
        <property type="entry name" value="SpoVAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07451">
        <property type="entry name" value="SpoVAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF011570">
        <property type="entry name" value="SpoVAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="344" mass="36073" checksum="3DAB09AF2C31DFCC" modified="2013-07-24" version="1">MANFIGQGVFRCSHPIPILATAAIAGEKEGQGPMSACFDRICSDSYFGQTTWEQAEGALQRETVSLAIEKAGLQPEQLDGILAGDLVNQCTSSSYGLRGLEVPFLGIYGACSTMSEGLLLASLLADSGAGRHFIAATSSHFSTAERQFRFPLSYGAQRTPTAQWTCTASGAAVLAATAETETPCITGGCIGRIQDLGVTDANNMGAAMAPAAADTYIRWFQASGTSPADYDAIVTGDLGIIGSNLFCDLLMKQGYDVTGIHQDCGAKMFDPETQDTHAGGSGCGCAGSLFCGHFYRQLETGTFRRILFAATGALLSPMLIQQGESIPSISHLVEVQGVVSRETT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5VQL3</accession>
    <name>R5VQL3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nitrogen regulatory protein P-II</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00669</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the P(II) protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000016">
        <property type="protein sequence ID" value="CCZ82867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00669"/>
    <dbReference type="EnsemblBacteria" id="CCZ82867">
        <property type="protein sequence ID" value="CCZ82867"/>
        <property type="gene ID" value="BN566_00669"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006808">
        <property type="term" value="P:regulation of nitrogen utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002187">
        <property type="entry name" value="N-reg_PII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017918">
        <property type="entry name" value="N-reg_PII_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30115">
        <property type="entry name" value="PTHR30115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00543">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00340">
        <property type="entry name" value="PIIGLNB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00938">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="SSF54913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00638">
        <property type="entry name" value="PII_GLNB_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51343">
        <property type="entry name" value="PII_GLNB_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="61" mass="6710" checksum="5A3D7A95812F60DB" modified="2013-07-24" version="1">MMNTLPKVYMMTVVPDAEVDGILKLIKQCVYTGTVGDGKIFISTVDECIRIRTNETGDTAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>R5VQQ0</accession>
    <name>R5VQQ0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Cardiolipin synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN566_00699</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000022">
        <property type="protein sequence ID" value="CCZ82897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00699"/>
    <dbReference type="EnsemblBacteria" id="CCZ82897">
        <property type="protein sequence ID" value="CCZ82897"/>
        <property type="gene ID" value="BN566_00699"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008808">
        <property type="term" value="F:cardiolipin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032049">
        <property type="term" value="P:cardiolipin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022924">
        <property type="entry name" value="Cardiolipin_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027379">
        <property type="entry name" value="CLS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025202">
        <property type="entry name" value="PLD-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13091">
        <property type="entry name" value="PLDc_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13396">
        <property type="entry name" value="PLDc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04265">
        <property type="entry name" value="bac_cardiolipin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="38"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="13">
        <location>
            <begin position="243"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="13">
        <location>
            <begin position="422"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CCZ82897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="509" mass="59588" checksum="EF0DABBA623AE614" modified="2013-07-24" version="1">MKKIWKHLFNQNMLYILLMLVQIAFLVLSVLFLNRNYAWVYGALLLLNGLLIVYITNSRKNPTYKNAWLVVIAVLPIFGGLSYLFLKTRRDTRIFFHNYKQQMEQTKPLLTQEPDAARHLFNDSQQVYNLSTYLQHTGFPVYEQTNVTYFPLGEEQFAEMKNRLREAKRFIFIEYFIIAPGKMWNEFLEILIERARAGVEVRLMYDGFGTKFFSTGDFLKIAKQHHIQCRCFNPFRPLLSSAQNNRDHRKILVIDGNIAFNGGTNLADEYINEKVRFGHWKDTAIMLEGAAVWSYTMMFLQLWNMNNPNRKEAYDQYRPTYLPKQTNGGFIQPYGDSPVQDDVKGVMVYLDLISNAKRYVYIETPYLIPDHDLLTALKLAAEKGVDVRIITPHIPDKWYVYQLAWNAYQELLESGVKIFEYTPGFIHAKSFVVDDELAVLGTINLDYRSLYLHFECATLIYHCNVIQTMLADFLKTQRKSQPITLEDCKNRKWYQKCNSYVLGLLAPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>R5VQS3</accession>
    <name>R5VQS3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">L-aspartate oxidase</fullName>
            <ecNumber evidence="4">1.4.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN566_00712</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-aspartate + O2 = H2O2 + iminosuccinate</text>
            <dbReference type="Rhea" id="RHEA:25876"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
            <dbReference type="EC" id="1.4.3.16"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:25877"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (oxidase route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.16" evidence="4"/>
    <dbReference type="EMBL" id="CBAR010000026">
        <property type="protein sequence ID" value="CCZ82912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00712"/>
    <dbReference type="EnsemblBacteria" id="CCZ82912">
        <property type="protein sequence ID" value="CCZ82912"/>
        <property type="gene ID" value="BN566_00712"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00326"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008734">
        <property type="term" value="F:L-aspartate oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044318">
        <property type="term" value="F:L-aspartate:fumarate oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.700.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-binding_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005288">
        <property type="entry name" value="NadB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027477">
        <property type="entry name" value="Succ_DH/fumarate_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42716">
        <property type="entry name" value="PTHR42716"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56425">
        <property type="entry name" value="SSF56425"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD_binding_2" evidence="10">
        <location>
            <begin position="6"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ82912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="484" mass="53956" checksum="8E4AB11194CBB4A6" modified="2013-07-24" version="1">MNHNFDVIIVGGGAAGLYGAIQLPSYCKVLLICKQEMTLCNSSLAQGGIAGVYKSPEDDTPELHKQDTMIAGGFKNNPESVQILVEDAAMAIDSLLKLGADFDRKPDGSLHRTLEGGHCRHRIFHHKDTTGEEIVRTLMRKVKTLPNVTILESAMMVGLQKTSTGFSINVRKDDAFTIYNSHFVLLATGGIGRMYAYTTNSKIATGDGIAFAHDLGAKILNLHLIQFHPTAFNDHHTRECFLISEAVRGEGAYLLNCNKERFMDRYDERLELAPRDVVSHAIIEEKNRTGSDNFFLDISYKDSDFIRNRFPMIYQKVLEQGYDMTKEPIPIYPCQHYLMGGIQVDTNSETTVPHLYAAGECSNTGVHGNNRLASNSLLEALVFSRRAALDIATKLPTVPDAFAEATFPETANTLPMPKGTRTEIRQIMQNSYFVIPHQAELLNGFERIKEIRRELYSEPYAVTPDLIEAKAMATNAYTILKEVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5VR65</accession>
    <name>R5VR65_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SGNH_hydro domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00820</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000040">
        <property type="protein sequence ID" value="CCZ83027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83027">
        <property type="protein sequence ID" value="CCZ83027"/>
        <property type="gene ID" value="BN566_00820"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013830">
        <property type="entry name" value="SGNH_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13472">
        <property type="entry name" value="Lipase_GDSL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SGNH_hydro" evidence="1">
        <location>
            <begin position="9"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="229" mass="26460" checksum="91EDD35A22485EA1" modified="2013-07-24" version="1">MEQTLQIGIFGDSISKGIVLDAETHRYSSLKKEITEWISPSTALHNFSVMGSTIQKGLSIITRHTDQLASYQNVFLEFGGNDCDFDWSAVSERPQAHHEPHTPLKQFEALYTKAIQLIQEKKGNPILLTLPPLEPKRYFQWISRNLHPDNILHWLGGVDTIYRWQEMYNMKVLLLAQKLHVPVVDIRSAFLAQHGYQALLCEDGIHPNRKGHRFIYKTIMQQYHPCLQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>R5VRB6</accession>
    <name>R5VRB6_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ion-translocating oxidoreductase complex subunit B</fullName>
            <ecNumber evidence="5">7.-.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Rnf electron transport complex subunit B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rnfB</name>
        <name evidence="9" type="ORF">BN566_00885</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Part of a membrane-bound complex that couples electron transfer with translocation of ions across the membrane.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="5">Binds 3 [4Fe-4S] clusters.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">The complex is composed of six subunits: RnfA, RnfB, RnfC, RnfD, RnfE and RnfG.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 4Fe4S bacterial-type ferredoxin family. RnfB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.-.-.-" evidence="5"/>
    <dbReference type="EMBL" id="CBAR010000054">
        <property type="protein sequence ID" value="CCZ83097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00885"/>
    <dbReference type="EnsemblBacteria" id="CCZ83097">
        <property type="protein sequence ID" value="CCZ83097"/>
        <property type="gene ID" value="BN566_00885"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00463">
        <property type="entry name" value="RsxB_RnfB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007202">
        <property type="entry name" value="4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010207">
        <property type="entry name" value="Elect_transpt_cplx_RnfB/RsxB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04060">
        <property type="entry name" value="FeS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01944">
        <property type="entry name" value="rnfB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51656">
        <property type="entry name" value="4FE4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="3 5" id="KW-0408">Iron</keyword>
    <keyword evidence="4 5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S" evidence="8">
        <location>
            <begin position="31"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="7">
        <location>
            <begin position="160"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="7">
        <location>
            <begin position="205"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="7">
        <location>
            <begin position="234"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Hydrophobic" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ83097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="284" mass="29761" checksum="10600227E5114F36" modified="2013-07-24" version="1">MEYILPIGIFIVFGIVFGVLLTVISKVFAVKVDERAQKITETLPGANCGACGYAGCADYADAIVNKGAPMNACLPGGAAAASKIGEIMGVAVTAAERKIPVVHCNGTCEATKPKFAFDGVQTCASAKRFYGGTGSCMHGCLGLGDCAAACEKDCIRIQDGLAVICTEECISCGKCAKACPNGLITLRSEKKKVDVLCSSKDMGKTAMQACQHACIGCKKCEKICKFDAIHVIDNHAVIDYDKCRSCGLCVKECPKQCIVKQVGKRPAIVKPKTDAEPAESAAQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5VRC8</accession>
    <name>R5VRC8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Xanthine permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_00899</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000059">
        <property type="protein sequence ID" value="CCZ83112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00899"/>
    <dbReference type="EnsemblBacteria" id="CCZ83112">
        <property type="protein sequence ID" value="CCZ83112"/>
        <property type="gene ID" value="BN566_00899"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015205">
        <property type="term" value="F:nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006043">
        <property type="entry name" value="NCS2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00860">
        <property type="entry name" value="Xan_ur_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="115"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="180"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="204"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="250"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="327"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ83112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="371" mass="38975" checksum="89BA043DF8F10A34" modified="2013-07-24" version="1">MKQKTEHQKETVENIYQLNGTVPIVKAIPFGLQHILAMFVANIAPILIVASACGLSDQNTAMLIQSAMLIAGIGTLVQLFPIFHKIGSGLPIVMGISFTFVSILCFVGTQYGYGAIMGAVLIGGIIEGILGLFAKYWLKLIAPIVSASVVTAIGFSLLSVGANSFGGGSGSEDFGSVQNWILGSVTLLCCILFHIFAKSYWKQLSVLFGLVVGYLLAIAMGAVDFSALKDTSIFAVPHLLPFKMEFHPDAILSVTLIFLVSATETIGDTSALAASGLNRDVTKEETSGSIACDGFVSALSSVFGCLPITSFSQNVGLVAMTKVVNRFTIATGAGIMILAGIFPFFGALLATLPDAVLGGCTNYCCQWLAND</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>R5VRK8</accession>
    <name>R5VRK8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 8">DNA-directed RNA polymerase subunit beta</fullName>
            <shortName evidence="6">RNAP subunit beta</shortName>
            <ecNumber evidence="6 8">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">RNA polymerase subunit beta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Transcriptase subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpoB</name>
        <name evidence="16" type="ORF">BN566_00975</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 8">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6 8">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6 8">The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="6 8"/>
    <dbReference type="EMBL" id="CBAR010000070">
        <property type="protein sequence ID" value="CCZ83192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00975"/>
    <dbReference type="EnsemblBacteria" id="CCZ83192">
        <property type="protein sequence ID" value="CCZ83192"/>
        <property type="gene ID" value="BN566_00975"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032549">
        <property type="term" value="F:ribonucleoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00653">
        <property type="entry name" value="RNA_pol_B_RPB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01321">
        <property type="entry name" value="RNApol_bact_RpoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042107">
        <property type="entry name" value="DNA-dir_RNA_pol_bsu_ext_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019462">
        <property type="entry name" value="DNA-dir_RNA_pol_bsu_external_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007120">
        <property type="entry name" value="DNA-dir_RNAP_su2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037033">
        <property type="entry name" value="DNA-dir_RNAP_su2_hyb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010243">
        <property type="entry name" value="RNA_pol_bsu_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007121">
        <property type="entry name" value="RNA_pol_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007644">
        <property type="entry name" value="RNA_pol_bsu_protrusion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007642">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037034">
        <property type="entry name" value="RNA_pol_Rpb2_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007645">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007641">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014724">
        <property type="entry name" value="RNA_pol_RPB2_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="PTHR20856"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04563">
        <property type="entry name" value="RNA_pol_Rpb2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04561">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04565">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10385">
        <property type="entry name" value="RNA_pol_Rpb2_45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00562">
        <property type="entry name" value="RNA_pol_Rpb2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04560">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02013">
        <property type="entry name" value="rpoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01166">
        <property type="entry name" value="RNA_POL_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6 8" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="3 6 8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RNA_pol_Rpb2_1" evidence="13">
        <location>
            <begin position="47"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="RNA_pol_Rpb2_2" evidence="12">
        <location>
            <begin position="141"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="RNA_pol_Rpb2_2" evidence="12">
        <location>
            <begin position="379"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="RNA_pol_Rpb2_3" evidence="14">
        <location>
            <begin position="510"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="domain" description="RNA_pol_Rpb2_45" evidence="15">
        <location>
            <begin position="588"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="domain" description="RNA_pol_Rpb2_6" evidence="10">
        <location>
            <begin position="717"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="domain" description="RNA_pol_Rpb2_7" evidence="11">
        <location>
            <begin position="1131"/>
            <end position="1205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1235"/>
            <end position="1298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1239"/>
            <end position="1265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="1268"/>
            <end position="1298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF04561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF04565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF10385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CCZ83192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="1298" mass="146162" checksum="9D8A2B1C3B7A3570" modified="2013-07-24" version="1">MVNVTPKQCGKNVRMNFGKINEVLEMPNLIEVQKNSYRWFREEGLKEVFRDVSSITDYNGNLVLNFIDYRIDEKPKYTIAECKEQDATYAAALRVTATLWNKETGEVKESEIYMGDFPLMTESGTFVINGAERVIVSQLVRSPGVYYASEKDRTGKDLFKTTVIPNRGAWLEYEMDSNDVIYVRIDKNRKIALTTFIRAIGIGTDEEIYEMFGDDERLRQTILQKDSTKNTEDALLDVYKKLRPGEPPTVESSLTHLNNLFFDAKRYDLCRFGRYKYNKKLGIASRLAGHTLSRPVINEMTGELLFDAGTTLTYEQAEQIQEAGVKAAFLTVECKEYYVTEAGENAIRFVQREVKVIGNGMVDLQPYVQFDISKYEINEKVSFSVLREILESSSTPEEVEEQVKKRLDELVPKHIILDDIYATISYFLNLCEGVGTVDDIDHLGNRRIRSVGELLQNQFRIGFTRMERVIRERMNIQAQDMDVITPQSLVNIRPITAAIKEFFGSSPLSQFMDQNNPLAELTHKRRLSALGPGGLSRDRAGFEVRDVHYSHYGRMCPIETPEGPNIGLISYLATFARINEYGFIEAPYRRVDKATGVVTDEVVYMTADVEDNYIVAQANEPLTEDGHFANHKVAARYREQILEVEQDRIDFMDVSPKMVVSVATSMIPFLENDDANRALMGSNMQRQAVPLLKTERPFVGTGMEYKAAVDSGVCVLAPCDGVITAVSADTITLLSDETHKEQTFKLEKFKRSNQGTCINQRPIVDAGEHVTKGQVLADGPSTSDGEISLGKNALIGFMTWEGYNYEDAVLLNERLVREDIFTSVHIEEYEIECRDTKLGPEEITRDIPNVGDDALKDLDENGIIRIGAEVHAGDILVGKVTPKGETELTAEERLLRAIFGEKAREVRDNSLKVPHGEAGIIIDVKIFTRENCDELSAGVNKLVRCYIAQKRKISVGDKMAGRHGNKGVVSRILPEEDMPFLPDGTPLDIVLNPLGVPSRMNIGQVLEVHLGMAAKALGWHVMTPVFDGAHEDDIRECFREANRRNAPHRNDPFDLKPMQKVINPKALEFCEDGKFTLYDGRTGEKFDNRVTVGYMHYLKLHHLVDEKIHARSVGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEILTVKSDDTVGRVETYEAIVKGQNVPKPGIPESFKVLIKELQSLGLDVKVLDGEQNEIDLRSNFDDDVTAQVVSYESDDDITDTANYEGMSKDMEDAGMGYDDDKTIANGDPDAEDVPFEDIDDTDEDDYPDDVDDYPFDDDEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5VRX1</accession>
    <name>R5VRX1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transporter major facilitator family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN566_01088</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01088"/>
    <dbReference type="EnsemblBacteria" id="CCZ83307">
        <property type="protein sequence ID" value="CCZ83307"/>
        <property type="gene ID" value="BN566_01088"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="55"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="79"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="108"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="136"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="208"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="245"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="275"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="299"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="330"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="365"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="8">
        <location>
            <begin position="13"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ83307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="394" mass="43014" checksum="A772AFFD2B0DC20D" modified="2013-07-24" version="1">MTKQKMPLKAWLPLIGMTVSAFIFNTSEFMPIGLLSDIAANFGITESHAGLLISVYSWVVTLLSLPLMLLVSKMSYRKLLLGTIALFGIFQVLSGVSASYGMLMVSRIGVACTHAIFWSVASPIAVRLVPEEHQTLAMSMIVTGTSVAMIFGLPLGRVIGLTVGWRMTFLCVAAVAFLVLFYLLFVFPKMSNGTPFQIRQLPQILKRRQLLGIYLITFLVASAYYTGYSYIEPFLKQVAHLADSWITIVLTLFGAAGLLGSFLFSRLYDKHRYAFIRFIIASVAVALLCLRVASISLYLVILVCVLWGMAVTAFNVAFQAETIKAVPEEASAVAMSLFSGIFNLGIGCGTWLGGTVCNQLSIANIGYAGGIIGVLAVLYCIFRMIPLWKAKQKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5VS96</accession>
    <name>R5VS96_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosomal_L7Ae domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01183</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000097">
        <property type="protein sequence ID" value="CCZ83407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01183"/>
    <dbReference type="EnsemblBacteria" id="CCZ83407">
        <property type="protein sequence ID" value="CCZ83407"/>
        <property type="gene ID" value="BN566_01183"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="L30e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_L7Ae/L30e/S12e/Gad45"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="SSF55315"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosomal_L7Ae" evidence="1">
        <location>
            <begin position="6"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="109" mass="11970" checksum="306ED68C2E6FFE12" modified="2013-07-24" version="1">MPLPEKCRNLLTICRKAGKLEAGFARAKDAIQGGYAACILLTADLSEKSRKEVLYFAEKQHVPVVQTDWEMAEVRLLFGQRAGILTICEAGFAQRIQALLQTKSSSSNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>R5VSD7</accession>
    <name>R5VSD7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Exodeoxyribonuclease 7 small subunit</fullName>
            <ecNumber evidence="3">3.1.11.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Exodeoxyribonuclease VII small subunit</fullName>
            <shortName evidence="3">Exonuclease VII small subunit</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">xseB</name>
        <name evidence="4" type="ORF">BN566_01216</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Bidirectionally degrades single-stranded DNA into large acid-insoluble oligonucleotides, which are then degraded further into small acid-soluble oligonucleotides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>Exonucleolytic cleavage in either 5'- to 3'- or 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.11.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterooligomer composed of large and small subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the XseB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.11.6" evidence="3"/>
    <dbReference type="EMBL" id="CBAR010000105">
        <property type="protein sequence ID" value="CCZ83442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01216"/>
    <dbReference type="EnsemblBacteria" id="CCZ83442">
        <property type="protein sequence ID" value="CCZ83442"/>
        <property type="gene ID" value="BN566_01216"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009318">
        <property type="term" value="C:exodeoxyribonuclease VII complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008855">
        <property type="term" value="F:exodeoxyribonuclease VII activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006308">
        <property type="term" value="P:DNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00337">
        <property type="entry name" value="Exonuc_7_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003761">
        <property type="entry name" value="Exonuc_VII_S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037004">
        <property type="entry name" value="Exonuc_VII_ssu_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34137">
        <property type="entry name" value="PTHR34137"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02609">
        <property type="entry name" value="Exonuc_VII_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006488">
        <property type="entry name" value="Exonuc_VII_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116842">
        <property type="entry name" value="SSF116842"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01280">
        <property type="entry name" value="xseB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="62" mass="7080" checksum="53A2BE29AD15A5AE" modified="2013-07-24" version="1">MTFEENLKQLQQVTEQLEQSTLPLEEALALYQKGVQLSDACQTALQEAEKQFSLTVEEEDFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VSE7</accession>
    <name>R5VSE7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01226</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000109">
        <property type="protein sequence ID" value="CCZ83452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83452">
        <property type="protein sequence ID" value="CCZ83452"/>
        <property type="gene ID" value="BN566_01226"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="133" mass="15099" checksum="1E6252B39CE863F4" modified="2013-07-24" version="1">MAYTCTVQEKRTFPVSIGSHIFYVEQYQLTGIRKFAEQTTIAGMSVFTNQAIRSRVLHMEGRFLRTDPPAELLLTLETALQEQTSFTAVLDGVRYRMCRLSQYRIQEDGHSGTVACQLDCLVSQLEAVEEKAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>R5VSS5</accession>
    <name>R5VSS5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Glycosyl hydrolase family 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN566_01350</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000132">
        <property type="protein sequence ID" value="CCZ83582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01350"/>
    <dbReference type="EnsemblBacteria" id="CCZ83582">
        <property type="protein sequence ID" value="CCZ83582"/>
        <property type="gene ID" value="BN566_01350"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030248">
        <property type="term" value="F:cellulose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008965">
        <property type="entry name" value="CBM2/CBM3_carb-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001956">
        <property type="entry name" value="CBM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036966">
        <property type="entry name" value="CBM3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002105">
        <property type="entry name" value="Dockerin_1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001701">
        <property type="entry name" value="Glyco_hydro_9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00942">
        <property type="entry name" value="CBM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00404">
        <property type="entry name" value="Dockerin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00759">
        <property type="entry name" value="Glyco_hydro_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01067">
        <property type="entry name" value="CBM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="SSF48208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49384">
        <property type="entry name" value="SSF49384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51172">
        <property type="entry name" value="CBM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00448">
        <property type="entry name" value="CLOS_CELLULOSOME_RPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004407362" evidence="6">
        <location>
            <begin position="32"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="domain" description="CBM3" evidence="7">
        <location>
            <begin position="588"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="8">
        <location>
            <begin position="753"/>
            <end position="813"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ83582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="813" mass="90822" checksum="0E4A1BC598204EE1" modified="2013-07-24" version="1">MKRNRCKKIAAALLCVVMGCTTLGQGFSAAAADATTSEEEVEINFAKALQMSLYFYDANKCGSGITGGNLEWRGDCHTEDAAVPLKPMGEDYKGTNLSQEFIDAHRDILDPDGDGTIDVAGGMHDAGDHVKFCLPGSYAASTLGWGYYEFRDAYVDSGQQWHIEDILHWFNDYYLKCTYFDENGDILAFCYQVGEGDIDHNYWNAPELQNESLLDFARPAYFATVETPASDMCAGVAASLAVNYLNFKDTEPEYAKRCLNTAVKMYEFAVKTHSEAEDGKTVTSLGYDGGFYTSSYDYDELAWAAVWLYECTGEYDYINDIIAVDENTSGDMGAHPYTGYLKRIIADTGNCWQNIWVHCWDTVWGGVFAKLAPITNTARDWYIFRWNLEYWSGLTHADPGGSDDVLWNLPYDPKTAPFVADTTDTTFLAKTPAGFSMLNNYGSCRYDAAAQLCALVYEKETGDDKFADWAEGQMEYIMGKNPMNRAYIVGYSKNAASHPHHRAAHGSKTLNMDEPADQTHVLWGALVGGPDEKDFHNDQTKDYIYNEVAVDYNAAFVGACAGLYQYYGKAMGNKPVENFPPSEASMKGDIQEIYIDARVMQETDARSQIEISICNDTSLPPRYISTLSARYFFNINEMLKAGQTIDDLDIEIYYDEENANTNGESFATISKPVKWDDNGTYYVEISWDGCAFYGTRVFHFGLIPKMDANYTTHWDPTDDYSRDGLILDEYTRTEKITAYQDGELVWGEEPEGGTVLLGDVNRDGTVNLPDIVLLQKHLCNKATLKKRNAVAADLNADGAITILDAVLLKRKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>R5VTM6</accession>
    <name>R5VTM6_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Glutamate racemase</fullName>
            <ecNumber evidence="2 7">5.1.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">murI</name>
        <name evidence="8" type="ORF">BN566_01606</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Provides the (R)-glutamate required for cell wall biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>L-glutamate = D-glutamate</text>
            <dbReference type="Rhea" id="RHEA:12813"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29986"/>
            <dbReference type="EC" id="5.1.1.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the aspartate/glutamate racemases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.1.1.3" evidence="2 7"/>
    <dbReference type="EMBL" id="CBAR010000169">
        <property type="protein sequence ID" value="CCZ83877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01606"/>
    <dbReference type="EnsemblBacteria" id="CCZ83877">
        <property type="protein sequence ID" value="CCZ83877"/>
        <property type="gene ID" value="BN566_01606"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008881">
        <property type="term" value="F:glutamate racemase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00258">
        <property type="entry name" value="Glu_racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015942">
        <property type="entry name" value="Asp/Glu/hydantoin_racemase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001920">
        <property type="entry name" value="Asp/Glu_race"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033134">
        <property type="entry name" value="Asp/Glu_racemase_AS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004391">
        <property type="entry name" value="Glu_race"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21198:SF2">
        <property type="entry name" value="PTHR21198:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01177">
        <property type="entry name" value="Asp_Glu_race"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53681">
        <property type="entry name" value="SSF53681"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00067">
        <property type="entry name" value="glut_race"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00924">
        <property type="entry name" value="ASP_GLU_RACEMASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0133">Cell shape</keyword>
    <keyword evidence="6 7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4 7" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="10"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="42"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="186"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="7">
        <location>
            <position position="73"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="7">
        <location>
            <position position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="273" mass="29877" checksum="959F8C5C378E3DF9" modified="2013-07-24" version="1">MKNDAIGVFDSGMGGLTTVKELNRLLPHENIIYFGDTARVPYGSRSPETILRYAKEDVAFLRQYQVKMIIAACGTVSSIVGDQPFVTDIPFAGVIYPAVEAACAVTKTKRIGIIGTSATIKSKSYERAILQQHPDFFVATQACPLFVHLVENGYTDFNNPVTRLVAEEYLTPIKEQKVDTLILGCTHYPVIADLIGDIMGRDVALISPGAETAGYAKKCLAEQNLLSDRTEKGKNTYYVSDSVSLFTENAAHFLQEEVSGAVYQSSWQKEAGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>R5VTT6</accession>
    <name>R5VTT6_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tryptophan synthase alpha chain</fullName>
            <ecNumber evidence="7">4.2.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">trpA</name>
        <name evidence="9" type="ORF">BN566_01661</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + H2O + L-tryptophan</text>
            <dbReference type="Rhea" id="RHEA:10532"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.2.1.20"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Tetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the TrpA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.20" evidence="7"/>
    <dbReference type="EMBL" id="CBAR010000173">
        <property type="protein sequence ID" value="CCZ83932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01661"/>
    <dbReference type="EnsemblBacteria" id="CCZ83932">
        <property type="protein sequence ID" value="CCZ83932"/>
        <property type="gene ID" value="BN566_01661"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00044"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004834">
        <property type="term" value="F:tryptophan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04724">
        <property type="entry name" value="Tryptophan_synthase_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00131">
        <property type="entry name" value="Trp_synth_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018204">
        <property type="entry name" value="Trp_synthase_alpha_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002028">
        <property type="entry name" value="Trp_synthase_suA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43406">
        <property type="entry name" value="PTHR43406"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00290">
        <property type="entry name" value="Trp_syntA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00262">
        <property type="entry name" value="trpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00167">
        <property type="entry name" value="TRP_SYNTHASE_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5 7" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ83932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="264" mass="29297" checksum="C066508E1704B2BB" modified="2013-07-24" version="1">MTENRIDRTLQALKQQGKKALIPFVTAGHGGYDRTEQLVLQMEKAGADIIELGVPFSDPIAEGPVIQHASEQALKQGTTLAGIFELVKRLRKKTDMPLLLMMYLNTIFSHGTEEFFRTCQEIGIDGVIVPDMPFEEKDEIQGSADKYHIHNISLVTPASEGRIQMIAEDATGFLYCVSSNGVTGMRTKFQTDFSAFFTPIHKYAKIPCMVGFGISSPEAAKQMSQYCDGVIVGSAIVNMVEQYQEQAPEKVYEFVKSLRTALDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5VU74</accession>
    <name>R5VU74_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01788</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000184">
        <property type="protein sequence ID" value="CCZ84062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01788"/>
    <dbReference type="EnsemblBacteria" id="CCZ84062">
        <property type="protein sequence ID" value="CCZ84062"/>
        <property type="gene ID" value="BN566_01788"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004395476" evidence="1">
        <location>
            <begin position="32"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="295" mass="31952" checksum="71E8BFDDF148D619" modified="2013-07-24" version="1">MKKRIFSMLLASCFLLITCSGCGDTSESSTASTEAATTAETVETAETVQVVQDGMQPIFGSDIKDGTYEITVDSSSTMFRITACQLTVKDGTMTADMTMGGTGYLYLYMGSSEDAEKADESDYIPFTENADGTHTFTVPVEALDSGIACAAFSKKKEIWYDRTLVFRADSLPMDARSDDAYTTVESLQLEDGTYTCSVQLEGGSGRASVTSPATITVKDGTATAEIVWSSSNYDYMKVDDTQYNPTNTEGNSTFEIPVLGFDYAMPVSADTTAMSTPHEIDYTLFFDSTTLEKQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5VUA5</accession>
    <name>R5VUA5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NADPH-dependent FMN reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01823</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000186">
        <property type="protein sequence ID" value="CCZ84097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01823"/>
    <dbReference type="EnsemblBacteria" id="CCZ84097">
        <property type="protein sequence ID" value="CCZ84097"/>
        <property type="gene ID" value="BN566_01823"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN_red" evidence="1">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="205" mass="22587" checksum="3A38E2970A36E1D4" modified="2013-07-24" version="1">MNVLLINGSPHANGNTAVALREMERIFQENQVETEWIQVGNKDIRGCVACYGCTKKGACVFDDLVNETAPKFEACDGMVIGSPVYYASANATLVAFLHRLFYSTPFDKRMKVGASVVAARRGGLSATFDELNKFFTISGMPVASSQYWNSIHGREPGEAKQDAEGLQTMRTLARNMTFLMKSIALGKEAYGLPEQEPRQGTNFIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5VUA7</accession>
    <name>R5VUA7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Alcohol dehydrogenase iron-dependent</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01828</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the iron-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000187">
        <property type="protein sequence ID" value="CCZ84102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01828"/>
    <dbReference type="EnsemblBacteria" id="CCZ84102">
        <property type="protein sequence ID" value="CCZ84102"/>
        <property type="gene ID" value="BN566_01828"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990362">
        <property type="term" value="F:butanol dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08187">
        <property type="entry name" value="BDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001670">
        <property type="entry name" value="ADH_Fe/GldA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044731">
        <property type="entry name" value="BDH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43633">
        <property type="entry name" value="PTHR43633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00465">
        <property type="entry name" value="Fe-ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe-ADH" evidence="3">
        <location>
            <begin position="3"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="333" mass="36775" checksum="053CD6C8D2EC5B09" modified="2013-07-24" version="1">MQECGKTIVEDAGVMPNPTVEKLKEGCERARAGKVDFILAVGGGSVCDYAKAVSVSVHCSEDPWEKFYIREEEPENEIIPVGCVLTMVGTGSEMNAGSVITNHEQKLKIGHVFGEEVLPKFSILNPVFTFTLPKYQMIAGFFDIMSHILEQYFSGTDDNTSDYLMEGLLKSLIHSAKIAVENPKDYEARSNIMWIATWALNTLVEKGKTTDWEVHMIGQAVGAYTDATHGMTLSAVSLPYYRYILQAGLPKFKRYAMNVWDVCPDGKTDEQIAEEGLACMEAYMKQLGLVMNLTDLGVTEDMLEGIADATLIMTGGYQVLTHEDVVAILKQAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5VUG7</accession>
    <name>R5VUG7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Lysophospholipase L1-like esterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01892</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000193">
        <property type="protein sequence ID" value="CCZ84167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01892"/>
    <dbReference type="EnsemblBacteria" id="CCZ84167">
        <property type="protein sequence ID" value="CCZ84167"/>
        <property type="gene ID" value="BN566_01892"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004395729" evidence="2">
        <location>
            <begin position="29"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="291"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="461"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="641" mass="69223" checksum="26B46EC7EE76DFB6" modified="2013-07-24" version="1">MYTKKKGLAVLIAAVTGLSSLAVGTVSAAPAAAENAEAVKILSLGDSITDGYWTSGAYRKYMYHDLEQMGYNIDMVGPKGSNSNTFTYNGQTVSYDDNNAGYSGYAIQEMTTKEHRSGILETIQGTWYNGQNMIAAYQPDIVLLQIGTNDILSNYNDGITDRLENLVNVILQDLDSDSTVFVSTIPDIDAYTRADWLGSYGINAWGSTQEEKDQLMETVTGCIDTYNTSIYNLVLKMQSEGKNVQFADINSVVDYQTDLHDGVHPNEAGYENMGNYWAGLLNDFFQNHGTNPKPVTTTTTTQPAVTTTTTTTTTAKTTTTQPATTKPTTTTSTTKATTTTTAVTVPDGATEIHLTGVKKGESYSLANYPDATAISFTFSGNLQYIGGAFVLGNWTKNQSYNANDLNGNTLTFPIDMTDIEQKTFTFFRWNGGDDIQIEDVTLYCGTAPATTTKVTTTTAKPTTTTTTTTTAKPTTTTTTTTTTAKPTTTTTTTTTTAKPTTTTTTTTTTAKPTTTTTTTTTTAKPTTTTTKATTTTAKVTTTTASHAKKVVLTDVQYDKTYSLEDYHPSDIKEIVLQLDGEVGYGFGGKLVLGGWAVQMDYGVADMKDDKTITFKITNPQDVMTVYGYWGNMKLKSVTLVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5VUY5</accession>
    <name>R5VUY5_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00245</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000215">
        <property type="protein sequence ID" value="CCZ84332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00245"/>
    <dbReference type="EnsemblBacteria" id="CCZ84332">
        <property type="protein sequence ID" value="CCZ84332"/>
        <property type="gene ID" value="BN566_00245"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008484">
        <property type="term" value="F:sulfuric ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00884">
        <property type="entry name" value="Sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="SSF53649"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="95"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="142"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="183"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfatase" evidence="3">
        <location>
            <begin position="275"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="597"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="601"/>
            <end position="618"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="618" mass="70587" checksum="385737B8E9E023B7" modified="2013-07-24" version="1">MESLHKIISALRAKICACNERRNENTLRFTMTNRMLLLLFPIFIVCMAELNQDKYLSKLVIFIADHPTIMLFNILIAGLIFLGALLLFRKGWLAMLLQSVLYMALSITELFKYNTNGNHLIMTDMKLFRSVKSLTSFAYIKITPRLIIFVGIVLAYIVVSFWFNPKFSLHTKRLRRMITGLSCLLACALVILVPTLSTPVYAFFGVDVSKADNTFILNEKFENNGFLAFFMQTGSENIANQLQEPVAYEEDKDSTVAQYLDEVTDQANFKDGVKPNVIEIMSESYADFRVFDELDIPDDVYAGLDEVAAKGVSGTAIAPTYASYTVRTEFELLFGLPVKSLNDPNMPQRMLLERQQPTIPSYYKAQGYSTAYVHPFQGSFYNRRSIYSRFSFDTMIFEDDFTVPVDLYGTYIDDNTVYNQIEQLITDTDEPLYLHATTMQNHQPYNQGTGATEFDNYLQWVKHSSDGLADFIDRLSKLDEPTIVLFIGDHFPSLRGEDGIYAQLGITSENCSTLYEQKYVMWNNFGLDMTKVQPQEEVSVFYLPYVMMQEVGMPKDSFTQSMMDEMPVTPIYSTNYAPGQERNEKLDTLTYDRILGDMLSPDAVEEEEPDSSTNTDTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>R5VV87</accession>
    <name>R5VV87_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ATP synthase gamma chain</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">ATP synthase F1 sector gamma subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">F-ATPase gamma subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">atpG</name>
        <name evidence="10" type="ORF">BN566_00329</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">Produces ATP from ADP in the presence of a proton gradient across the membrane. The gamma chain is believed to be important in regulating ATPase activity and the flow of protons through the CF(0) complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the ATPase gamma chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000225">
        <property type="protein sequence ID" value="CCZ84422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00329"/>
    <dbReference type="EnsemblBacteria" id="CCZ84422">
        <property type="protein sequence ID" value="CCZ84422"/>
        <property type="gene ID" value="BN566_00329"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045261">
        <property type="term" value="C:proton-transporting ATP synthase complex, catalytic core F(1)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12151">
        <property type="entry name" value="F1-ATPase_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00815">
        <property type="entry name" value="ATP_synth_gamma_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035968">
        <property type="entry name" value="ATP_synth_F1_ATPase_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000131">
        <property type="entry name" value="ATP_synth_F1_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023632">
        <property type="entry name" value="ATP_synth_F1_gsu_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11693">
        <property type="entry name" value="PTHR11693"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00231">
        <property type="entry name" value="ATP-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00126">
        <property type="entry name" value="ATPASEGAMMA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52943">
        <property type="entry name" value="SSF52943"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01146">
        <property type="entry name" value="ATPsyn_F1gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00153">
        <property type="entry name" value="ATPASE_GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0139">CF(1)</keyword>
    <keyword evidence="4 9" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="5 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ84422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="297" mass="34147" checksum="C9746CE7EBE7FDD6" modified="2013-07-24" version="1">MSNMREIRERIKSINDIMKITNAMYLISSSKLKKAKKDLAATEPYFDKLLYAMRSILSRAPEDIDMRFFDTRTEIPADKRKKAFIIITADKGMCGSYNHNVIKRAEAEMEQCEYARLFVMGQVGRMYFQRKAREGELDVDAEFIQTTQNPTLYRAMKIAVLMMELFEQKKVDDVYIVYTKMLNSMTFEPKVQQLLPLNVNNFVTSEQDVTRHHEATFYPTVEAVMEHLTPNFMKGLIYGAMVESFCSEQQARMSAMDSATTSAKDMLQTLSLQYNRARQAAITQEITEISSGAEAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVE6</accession>
    <name>R5VVE6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00371</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000238">
        <property type="protein sequence ID" value="CCZ84472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84472">
        <property type="protein sequence ID" value="CCZ84472"/>
        <property type="gene ID" value="BN566_00371"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011737">
        <property type="entry name" value="CHP02206_TP0381"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09529">
        <property type="entry name" value="Intg_mem_TP0381"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="130"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="212"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="246" mass="28254" checksum="DDE848BF711E51CA" modified="2013-07-24" version="1">MSFFTAQEQMPAGKGFALFHGQHVAILLLIGGVLACGVWRYRKSVHRNAWRWGIVMTLCLLEVLKQVVLLTTHQFGKQYLPFDLCGISILFCLLEIWKPCAYFGELLYSLSLPGTLLALFFPNWNRLPLWNFFCIHSFLFHTLLLLLPVLLLADGTLQPNWKRLPLSFLTLAACSVVVAWLNRLWGTNFFFLSQPSKGSPLVWFADWFGASLYWIGFPILIVIIWVLLYVPVGIFQHRKQKSRSCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVF5</accession>
    <name>R5VVF5_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Shikimate kinase</fullName>
            <shortName evidence="10">SK</shortName>
            <ecNumber evidence="3 10">2.7.1.71</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">aroK</name>
        <name evidence="11" type="ORF">BN566_00381</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>ATP + shikimate = 3-phosphoshikimate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13121"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.71"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="10">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the shikimate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.71" evidence="3 10"/>
    <dbReference type="EMBL" id="CBAR010000240">
        <property type="protein sequence ID" value="CCZ84482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00381"/>
    <dbReference type="EnsemblBacteria" id="CCZ84482">
        <property type="protein sequence ID" value="CCZ84482"/>
        <property type="gene ID" value="BN566_00381"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00088"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004765">
        <property type="term" value="F:shikimate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00464">
        <property type="entry name" value="SK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00109">
        <property type="entry name" value="Shikimate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031322">
        <property type="entry name" value="Shikimate/glucono_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000623">
        <property type="entry name" value="Shikimate_kinase/TSH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023000">
        <property type="entry name" value="Shikimate_kinase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01202">
        <property type="entry name" value="SKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01128">
        <property type="entry name" value="SHIKIMATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9 10" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 10 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="10"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ84482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="176" mass="20097" checksum="9D3E9D80235FC0F5" modified="2013-07-24" version="1">MTLFLCGFMGCGKTALGKALAKRLELPLVDMDDLIVEREGMTIPEIFDQKGEPYFRKVESKLVEELAHTPQIVSCGGGVMTNPLNALIVRQCDGVIVYLQQKFAVCYERIKDDPNRPIVQSHTKEELQRIFDNRERWYQVNATYSVLAGETPEESADRVMRILKKWSPAFLEQQSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVV6</accession>
    <name>R5VVV6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted metal-dependent enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00510</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000266">
        <property type="protein sequence ID" value="CCZ84657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00510"/>
    <dbReference type="EnsemblBacteria" id="CCZ84657">
        <property type="protein sequence ID" value="CCZ84657"/>
        <property type="gene ID" value="BN566_00510"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010787">
        <property type="entry name" value="DUF1385"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07136">
        <property type="entry name" value="DUF1385"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="119"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="228"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="255"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="77"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="327" mass="37250" checksum="50148200B89C8B62" modified="2013-07-24" version="1">MKQTTKPEQAKHRSKIGGQALIEGVMMKGVHTGAMACRLPDGTIDVETWEEHNGKEMPWYRKCPFVRGSFNFVISMRDGYRCLMKSAEKQMTEEEKENEPQEEMNRFERWLNDKLGDKVFGIIMSISMVIGVAFAVFAFIFLPKFLIGLLITHTAIPDDSRFVRSLLEGVLKIIIFLGYMAFTGCMKDIRRTYEYHGAEHKTIACFEAGDPLTVENVKKHCRFHPRCGTSFIFITLIVSILVMCCVPLTVVWQRVLVSILLLPLVVGISYEFIRIAGRSDSWITRALSFPGLQIQRITTREPDASQIECAIAALKPCIPDNLEEDEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5UWR8</accession>
    <name>R5UWR8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Integral membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00573</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000007">
        <property type="protein sequence ID" value="CCZ82768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82768">
        <property type="protein sequence ID" value="CCZ82768"/>
        <property type="gene ID" value="BN566_00573"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="140" mass="15652" checksum="BA76F392D14E2917" modified="2013-07-24" version="1">MHTQQDSDAALQNNLTISEKSVVPYTHSSGYAIASLILGIISSALACVPILSTICSILGIIFVIKVRKREKVFHNGLVTAGLILCICGFVLSLLFLAYWIWYLFVFLCTVETQPHHEAPPPMPKYHGHPAKFLLSFYFQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5UWW5</accession>
    <name>R5UWW5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Stage II sporulation protein P</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00618</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000009">
        <property type="protein sequence ID" value="CCZ82813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00618"/>
    <dbReference type="EnsemblBacteria" id="CCZ82813">
        <property type="protein sequence ID" value="CCZ82813"/>
        <property type="gene ID" value="BN566_00618"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010897">
        <property type="entry name" value="Spore_II_P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07454">
        <property type="entry name" value="SpoIIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02867">
        <property type="entry name" value="spore_II_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004394429" evidence="1">
        <location>
            <begin position="35"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="374" mass="41727" checksum="D7D40016DCCE9C93" modified="2013-07-24" version="1">MRRKRRHRRKKRIWGVAVCLLGILCSVPAWHVSADPQKEPESQSDTVDTALQNVRSEGCVPLPVWNPLQEKAEQITMQAAETQLADSGQVEHPSVDASVVPYPTSWNIQETCDVIRTTYENYTGDAFFTLPNGGMVKNKTDHSNAELLAESELPPAFRIAVNDQPQVLIMHTHTTETYEPEVREHYDPQFSYRTTDCSKNVVAVGDAITAELQAAGIGVIHSDAVHDYPSYNGAYDRSAETVRGILAEYPSIKVVLDIHRDAICSDTTLSQPIVTIDGKEAAQIMIISGCDDGTMNMPNYLMNFRFACRLQSQMEQQYPNFTRPILFDYRHYNQDLTTGSLLIEVGSHGNTVEQAVYSGHLIGKSLAEVLLDLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5UWZ5</accession>
    <name>R5UWZ5_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Polysacc_synt_C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_00643</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000012">
        <property type="protein sequence ID" value="CCZ82838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00643"/>
    <dbReference type="EnsemblBacteria" id="CCZ82838">
        <property type="protein sequence ID" value="CCZ82838"/>
        <property type="gene ID" value="BN566_00643"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024923">
        <property type="entry name" value="PG_synth_SpoVB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029303">
        <property type="entry name" value="Polysacc_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002797">
        <property type="entry name" value="Polysacc_synth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01943">
        <property type="entry name" value="Polysacc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14667">
        <property type="entry name" value="Polysacc_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038958">
        <property type="entry name" value="PG_synth_SpoVB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="40"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="90"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="120"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="244"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="297"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="350"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="393"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="447"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="481"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="506"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="Polysacc_synt_C" evidence="7">
        <location>
            <begin position="391"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ82838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="546" mass="58135" checksum="635A1B929D3C237C" modified="2013-07-24" version="1">MKTQTFLKGSFILFLSALITKALGALFKIPLTMLLGGVGMGYFGCAYGLFLPIYALSITGLSTAVASLVAKEHGAGHIQNVRKIRRTARLLFAGVGLLLSGFIWLFAVPFARSTAADASAAYAIRMMAPAAFFGCLAAVERGYYEGLCNMYPTAISQAVESIAKVACGLLFSHYVFTHETQVLAYFPAGTPILPIAAAAAVLGVTLSTACGVLYFFFRNLGGDGLPKAASSSASLSIGSLMRSLFQIMLPVALGSFVTNLTSLIDLCTMMRCFDHLQQTQAAALFQKFGAVAAESTFPALVYGAFSGMALTVFNLVPSVTNMFGKSVLPCAAQSWAKGQKEAVQKQLQSVLFLTALIALPAAGGLFCLSDSILTLLYPGQETACRIAAESLRSLLPGMVFLCFTMPLFSILQGIGRADLPVKYMLPCVAVKLLGNILFLQIPACSLLGAGLSTSLCYALLFGMTLFSLCRMLGMSLRGLKPILPLCYAATMSITTAYLFRNLTRTMPLPVMLLCSVGSAVVMYGVVLLLLEKTANRLREGLCFRMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXJ4</accession>
    <name>R5UXJ4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Lyzozyme M1 (1 4-beta-N-acetylmuramidase)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_00600</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 25 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000008">
        <property type="protein sequence ID" value="CCZ82795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82795">
        <property type="protein sequence ID" value="CCZ82795"/>
        <property type="gene ID" value="BN566_00600"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003796">
        <property type="term" value="F:lysozyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016998">
        <property type="term" value="P:cell wall macromolecule catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009253">
        <property type="term" value="P:peptidoglycan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002053">
        <property type="entry name" value="Glyco_hydro_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018077">
        <property type="entry name" value="Glyco_hydro_fam25_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01183">
        <property type="entry name" value="Glyco_hydro_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00641">
        <property type="entry name" value="Glyco_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51904">
        <property type="entry name" value="GLYCOSYL_HYDROL_F25_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004406527" evidence="5">
        <location>
            <begin position="30"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="96"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="139"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="186"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="242"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="149"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ82795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="519" mass="56515" checksum="F5253FB24A2EE82E" modified="2013-07-24" version="1">MKLQWWKRSCSVLLAAAMGYFSIGMPVQAEEVPILEEQPEQLEPEATEAIEIEADAVEDPNVDVTEGSLLQAAIDAGYMENGKFVLTFYAKKASGTPIELTDAEINYYDVDGNGSIDVNDAVCFLTMYARKAVGLDPVPPEGWPLTPPNPNESEATETTTTTTTTTTTEETTTTTTIPTTTTITTTTTLPTTTTTTTLPTTTSTTTTAAKVTTTQKTTKASTTTAKVTTQKITTMAGTTTATGKTTAQSGTTTATGKTTAQSGTTTATGKTTTKARTTTVTTKATTTTAKTTAKPAATTTVITYATKPGIGAGAIFEGVDVSVYQGNIDWNKAKADGIEFAIMRAGYGKYVSQKDKYFDQNMKNAKAAGLPCGVYWFSYALTPEDAIKEADACYEVIKNYKLEYPVSFDMETESQMKLPKETVAQIIEAFCGRMESYGYYTTLYTYASFLNYKVEDRIFDKYDIWVAHYNTNKPAFNRNYGLWQYSCTGSVWGITGNVDRDYVYLDYERIIKNAHLNGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>R5UY04</accession>
    <name>R5UY04_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Type III pantothenate kinase</fullName>
            <ecNumber evidence="6">2.7.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">PanK-III</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Pantothenic acid kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">coaX</name>
        <name evidence="7" type="ORF">BN566_00745</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the phosphorylation of pantothenate (Pan), the first step in CoA biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>(R)-pantothenate + ATP = (R)-4'-phosphopantothenate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16373"/>
            <dbReference type="ChEBI" id="CHEBI:10986"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29032"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.33"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>NH4(+)</name>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
        </cofactor>
        <cofactor evidence="6">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="6">A monovalent cation. Ammonium or potassium.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 6">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 1/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the type III pantothenate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.33" evidence="6"/>
    <dbReference type="EMBL" id="CBAR010000030">
        <property type="protein sequence ID" value="CCZ82945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00745"/>
    <dbReference type="EnsemblBacteria" id="CCZ82945">
        <property type="protein sequence ID" value="CCZ82945"/>
        <property type="gene ID" value="BN566_00745"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00241">
        <property type="reaction ID" value="UER00352"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004594">
        <property type="term" value="F:pantothenate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01274">
        <property type="entry name" value="Pantothen_kinase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004619">
        <property type="entry name" value="Type_III_PanK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34265">
        <property type="entry name" value="PTHR34265"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03309">
        <property type="entry name" value="Pan_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00671">
        <property type="entry name" value="baf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 6" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="4 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0630">Potassium</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="107"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="109"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="6"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>a monovalent cation</name>
            <dbReference type="ChEBI" id="CHEBI:60242"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ82945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="254" mass="26964" checksum="3403D47E14128C2A" modified="2013-07-24" version="1">MILAIDIGNTNIVLGGFEGETILFIERISTNQAATDLEYASDVKTALDVHNIDPKEVQGAILSSVVPSVTNTFQRAVQKYLNVSVTVVEPGMKTGLSIVINNPAQLGSDLVVGAVAGIQEYSLPLIIIDMGTATTLSVINRNKQYLGGIIMPGVGVSHDSLIARTAQLPKIALEPPKHVIGKNTIDCMKSGLLFGTACSLDGLIDRIQAELGEPCTVVATGGLATVIVPLCKHPMILDDDLLLKGLMILYHRNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5UY27</accession>
    <name>R5UY27_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Geranylgeranyl reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00765</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000032">
        <property type="protein sequence ID" value="CCZ82965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00765"/>
    <dbReference type="EnsemblBacteria" id="CCZ82965">
        <property type="protein sequence ID" value="CCZ82965"/>
        <property type="gene ID" value="BN566_00765"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD_binding_3" evidence="1">
        <location>
            <begin position="4"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="299" mass="33580" checksum="BA638F1E1D69C8FF" modified="2013-07-24" version="1">MIYDAIIIGAGVAGLTCAMKLKQLGKSCIILEKRSLTTAKACGGGITNNALSLLSTLGIHTNEFLNADAKIITKVNQSFPDGKTEIYYYKNSSTKIKHSVGIRRKKFDEILLSHAAKNKIRIITGFDTNQIINNNYIHINDYIGKNLIYATGSIQQCYPSTHLKTFGISTELKAEMNVSDNTFYFFIDKSYYGGYAWIFPNGTNCWNIGVWQRNHFKLLKENFKKFYTTILTKKITHFTSMELPKSGSIHCGNKEIYIKHNNVYYIGECIGFASEQNGEGIYQAILSGISAAMDLYNTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYF4</accession>
    <name>R5UYF4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Acetyltransferase GNAT family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01129</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000092">
        <property type="protein sequence ID" value="CCZ83353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01129"/>
    <dbReference type="EnsemblBacteria" id="CCZ83353">
        <property type="protein sequence ID" value="CCZ83353"/>
        <property type="gene ID" value="BN566_01129"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="8"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="189" mass="22217" checksum="9671B025FC49BE11" modified="2013-07-24" version="1">MIIETERLVLRPFTEEDAGDVLEYLREPAVNCFACMKLNFLEEAKAEMQKRCGETEYYFAITLKDTGKVIGEIEAYPETGEQHSDENDPKDTFSPCWMLNKDYQGKGYAYEAAHAFFDYLFKDKGARRIYAYTEDYNTASQHLCEKLGMRREGLFQEFISFVNNPDGTPRYENTYQYAILKREWFNNQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYK0</accession>
    <name>R5UYK0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ig-like domain-containing surface protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_00892</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000059">
        <property type="protein sequence ID" value="CCZ83105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00892"/>
    <dbReference type="EnsemblBacteria" id="CCZ83105">
        <property type="protein sequence ID" value="CCZ83105"/>
        <property type="gene ID" value="BN566_00892"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026906">
        <property type="entry name" value="LRR_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13306">
        <property type="entry name" value="LRR_5"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004406561" evidence="3">
        <location>
            <begin position="32"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="4">
        <location>
            <begin position="619"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="588"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="701" mass="76527" checksum="51CC748FAA13AFE0" modified="2013-07-24" version="1">MKVTSRKTIAMLSAGILTLSCIPAEMFPVSATSAVTENALQLGAPVTIDNNLVCKVLEQKEDETFLLKITDVKDTTITSLTFPEEYDGYPIQFVELGDSVFKNCTNLTEITLPDEISAIPQQTFYGCTSLTTVNASNVTEIESEAFRNCTSLTTLNVSENVVKMGADALYHTAIFNEQYDEDGNEAVIKYFNNWVIGLKSNLSKEEKDIVIQEGTVGIANSSIRYINSVQLPSTLKYIGDKAFQNQYPYINLSEMNLPEGLISIGSYAFESTDLKEITIPSTVESVGSYAFSKTKIETVVVESGVKEINDYAFNDCSNLKTVTIGDNVQKMGTYVFSNDTALTDVTIGDGTEEIGERAFEGDRALLNVTIGEGLKELQYRIFAKCTFLKKIDLPDSLTFISNGAFSDSALMENGLSPLKYADNWLIGYNTNHTDDSVVTVKKGTVGIASEALYKHQMETLNLPDSLKYINSGAISYTSNLKTVTLPQGLERIESDAFKLSGLSEITIPESVEYIGDAAFYQEPWNSNFEKITILNPNCEIYDSYNSYTLIGKMNVFTIYGFNVSTAHEYANHNDRNFVALDAVETTTTTEATTTTTTTTTTTTTAPVTTTAPTTTTTEAELMQGDVDGDDVISVSDAVDVLKIYAMSAAGMDVSDFTAAQFQAADVDGDGVVAVSDAVYLLTYYARKAAGIDVTWDDIVNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYS7</accession>
    <name>R5UYS7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Heat-inducible transcription repressor HrcA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">hrcA</name>
        <name evidence="7" type="ORF">BN566_01237</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Negative regulator of class I heat shock genes (grpE-dnaK-dnaJ and groELS operons). Prevents heat-shock induction of these operons.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the HrcA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000111">
        <property type="protein sequence ID" value="CCZ83463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01237"/>
    <dbReference type="EnsemblBacteria" id="CCZ83463">
        <property type="protein sequence ID" value="CCZ83463"/>
        <property type="gene ID" value="BN566_01237"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00081">
        <property type="entry name" value="HrcA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002571">
        <property type="entry name" value="HrcA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021153">
        <property type="entry name" value="HrcA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023120">
        <property type="entry name" value="WHTH_transcript_rep_HrcA_IDD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34824">
        <property type="entry name" value="PTHR34824"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01628">
        <property type="entry name" value="HrcA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005485">
        <property type="entry name" value="HrcA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00331">
        <property type="entry name" value="hrcA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0678">Repressor</keyword>
    <keyword evidence="3 5" id="KW-0346">Stress response</keyword>
    <keyword evidence="4 5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HrcA" evidence="6">
        <location>
            <begin position="62"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ83463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="298" mass="33386" checksum="207939ECC92EBD7D" modified="2013-07-24" version="1">MAVLEELGYLEQPHTSAGRVPTYSGYRFYIDEVVEQGSLSDAERKRLDAMLGDESQLTEDLLIQSATTALSEVTKCATVVSDFAPKYSVISKVEVIPTGKRLYVVLLITSNGTIKNKACRLEFDLTEENMQFFRNFMEENLQGVSISDLSEERMEQMITAVSAYMMALSPLVQGVCEMSRDLQKNKVYVSGEQNLLSCQDLNQAEVVQFISHKNELRGLLDDSFKGVKVVFGEEGDRFVIGNSSMIVSKYQKGEQAVGSLGIIGPMRLDYAKIIPYIEYFTEKLSHLISEEDDEADNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYZ8</accession>
    <name>R5UYZ8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Succinate dehydrogenase/fumarate reductase flavoprotein subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_01056</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01056"/>
    <dbReference type="EnsemblBacteria" id="CCZ83275">
        <property type="protein sequence ID" value="CCZ83275"/>
        <property type="gene ID" value="BN566_01056"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008152">
        <property type="term" value="P:metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.700.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-binding_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037099">
        <property type="entry name" value="Fum_R/Succ_DH_flav-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015939">
        <property type="entry name" value="Fum_Rdtase/Succ_DH_flav-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030664">
        <property type="entry name" value="SdhA/FrdA/AprA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027477">
        <property type="entry name" value="Succ_DH/fumarate_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632">
        <property type="entry name" value="PTHR11632"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02910">
        <property type="entry name" value="Succ_DH_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46977">
        <property type="entry name" value="SSF46977"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56425">
        <property type="entry name" value="SSF56425"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD_binding_2" evidence="3">
        <location>
            <begin position="13"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Succ_DH_flav_C" evidence="4">
        <location>
            <begin position="439"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="564" mass="61961" checksum="D6257F6F75F9A312" modified="2013-07-24" version="1">MGDTVKTERLQTDVLVIGGGTAGCYAALTIAEQNAAVSVLICEKAHIKRSGCLAAGVNALNAYITKGRVPQDYVDYAKKDADGIVREDLLLTMSERLNAVTAKLEDLGLVLLKDADGNYVTRGNRNLKINGENIKPILADAVSALPNTTVLNRVNIVDFAVKDNRICGAFGIGIENDTFYVIEANAVLIATGGAAGLYKPNNPGFSRHKMWYPPFNTGAGYAMGIRAGAEMTTFEMRFIALRCKDTIAPTGTLAQGVGAKQINSHGEVYETKYGLTTSERVYGTVNENVEGRGPCYLRTEGITPEQDESLLKAYLNMAPSQTLKWIESGKNPSQQNVEIEGTEPYIVGGHTASGYWVDTHRATTVTGLFAAGDVAGGCPQKYVTGALAEGEIAACSALEFLKQHTAAPIAESSVTEHAEEVQRFLTQSGSRFTTEQLEEAMQTVMDAYAGGIKTNYRFNEKQLKIADEKIVQLMQLSEQLHADDFQELMYIYELRERLVVCRSVIAHLKARQETRWHSFAENLDYPEKDDVHWRKYVNSKLENGELKIIFRDLVEGGQTYEHCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZ68</accession>
    <name>R5UZ68_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">4Fe-4S binding domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_01116</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000089">
        <property type="protein sequence ID" value="CCZ83335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01116"/>
    <dbReference type="EnsemblBacteria" id="CCZ83335">
        <property type="protein sequence ID" value="CCZ83335"/>
        <property type="gene ID" value="BN566_01116"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12801">
        <property type="entry name" value="Fer4_5"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="85"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="134"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="188"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="234"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="259"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ83335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="307" mass="34289" checksum="C8C6CD41AB15C89D" modified="2013-07-24" version="1">MAKKKQRNKKKVHLLARFRGWIQAGATLLTNIHLPNFLNGSLNQGKGKYACVPGLNCYSCPGAAGSCPIGAFQAVVGSSKFKFSYYITGMLILFGVLLGRFICGFLCPFGWFQELLHKIPSPKFSTKKLKPLRYLKYGILLVMVILLPIVIVNEMGMGDPFFCKYLCPQGVLEGALPLSITNEGIRSALGVLFSWKAFVLLTVIVGSVFFYRPFCKWLCPLGAFYALLNRVSLFQMKVDKDKCVSCGACEKACKMDVDVTKHPNHAECIRCGMCMKSCPTNAIRYQYGFEKSSKTKKLTTEKKEFQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZD4</accession>
    <name>R5UZD4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF58 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01166</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000094">
        <property type="protein sequence ID" value="CCZ83390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01166"/>
    <dbReference type="EnsemblBacteria" id="CCZ83390">
        <property type="protein sequence ID" value="CCZ83390"/>
        <property type="gene ID" value="BN566_01166"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002881">
        <property type="entry name" value="DUF58"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01882">
        <property type="entry name" value="DUF58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="domain" description="DUF58" evidence="2">
        <location>
            <begin position="194"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="387" mass="43897" checksum="F1DA9313CCC74A30" modified="2013-07-24" version="1">MWYTKLIYLVLLIIGLFFSVLYEGIFSMLLLGVLILLPLTQLWLVHHVRRRLSVSLSLAQTEMGRGTSQKLYCTLQNKSHFPLNYCHVTLNLQQVATKETEQLELQLPLQGNFAATVSFQFAAMHCGKLRISVKKCRIADYFHLFSCSVRKCTAAEGIVVPSEQAGSLSMPNLQRSEMEDSVNYDPNRPGDDNTELFGIREFRDTDNPKRIHWKRSSREETLFVKEYSRPLEKQCAIWIDRTQTKAMQITGAKVDAQMEAAHALACILMRQQVPVVLFWNTEQGTQHETIETLEQLQRCMVQVLESPIPTESTGFQPVSEPATYAELFCITHAAELDTNAYRRTAEQILVLSTDETVLQQAAVFPISMETLLETLAAVCNPGTERSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZN5</accession>
    <name>R5UZN5_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Stage 0 sporulation protein A homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN566_01244</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000111">
        <property type="protein sequence ID" value="CCZ83470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01244"/>
    <dbReference type="EnsemblBacteria" id="CCZ83470">
        <property type="protein sequence ID" value="CCZ83470"/>
        <property type="gene ID" value="BN566_01244"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3 8 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Response regulatory" evidence="9">
        <location>
            <begin position="5"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="10">
        <location>
            <begin position="130"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="8">
        <location>
            <begin position="130"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="7">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ83470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="232" mass="26624" checksum="9D16C51292824BDC" modified="2013-07-24" version="1">MALEKVLVVDDDPNICDVLMMYLENEGYSVILSYDGEEALVKFNALKPDIVLLDVMLPSMDGWQVCREIRKKSSVPIIMITAKGDTFDKVLGLELGADDYIVKPFDTKEVVARIKAIARRVSAAPAETEVREVRYDKLSVNMTRYELRVNGKVVDTPPKELELLFYLASNPNRVYTRDQLLDEVWGFEYYGDSRTIDVHIKRLREKLEGVSDKWALKTVWGVGYKFESEEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZZ8</accession>
    <name>R5UZZ8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00132</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000124">
        <property type="protein sequence ID" value="CCZ83550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00132"/>
    <dbReference type="EnsemblBacteria" id="CCZ83550">
        <property type="protein sequence ID" value="CCZ83550"/>
        <property type="gene ID" value="BN566_00132"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="118" mass="12869" checksum="47DB4B14DDD0AC4D" modified="2013-07-24" version="1">MRELFLQIVYGRSQSAFSEGGLVVGAGLEDLGKGLRSQVGTMYGTLAKGPRYLEMTDGYVTGLALNEDDEIIGYKFVNFGKMMDFIKAGDDANTAFEKAQGQYGRVADAVKIVDPRKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5V072</accession>
    <name>R5V072_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01386</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000147">
        <property type="protein sequence ID" value="CCZ83650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01386"/>
    <dbReference type="EnsemblBacteria" id="CCZ83650">
        <property type="protein sequence ID" value="CCZ83650"/>
        <property type="gene ID" value="BN566_01386"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="7"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="74" mass="8329" checksum="039A69416402DDC9" modified="2013-07-24" version="1">MLIAESFKKTRIRHELTQVQLAKALGISDKMVSQIENGFRQPSAEILRNMAEQLGCSADEILGVQQQEKATDRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0C3</accession>
    <name>R5V0C3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00173</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000179">
        <property type="protein sequence ID" value="CCZ84013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84013">
        <property type="protein sequence ID" value="CCZ84013"/>
        <property type="gene ID" value="BN566_00173"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="122"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="167" mass="19062" checksum="39F58317705393A6" modified="2013-07-24" version="1">MAINIEDIMSEIRSDIQEKGLNSSMLSFADVPCDANPENPTDAYDPDTLRSNVQYVSTQYQMQPYRPLAGNPVMVFFKKVLRRLMRFYIEPLAQEQTYFNANTAQALQEMELYIIDTQAHSNAALAERIADLELQQKNSRMQIDALTKQVDALQAQLHQTETKEAAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0Q1</accession>
    <name>R5V0Q1_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">Cell shape-determining protein MreC</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4 5">Cell shape protein MreC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_01580</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in formation and maintenance of cell shape.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the MreC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000166">
        <property type="protein sequence ID" value="CCZ83845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01580"/>
    <dbReference type="EnsemblBacteria" id="CCZ83845">
        <property type="protein sequence ID" value="CCZ83845"/>
        <property type="gene ID" value="BN566_01580"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042177">
        <property type="entry name" value="Cell/Rod_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042175">
        <property type="entry name" value="Cell/Rod_MreC_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007221">
        <property type="entry name" value="MreC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34138">
        <property type="entry name" value="PTHR34138"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04085">
        <property type="entry name" value="MreC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038471">
        <property type="entry name" value="MreC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0133">Cell shape</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="66"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00032089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR038471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ83845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="324" mass="35060" checksum="64467920650D27F2" modified="2013-07-24" version="1">MGDFFKRNRLRFLLCILALLIGVLLYAAMQGGQTTGVMGVVLNPLRQVTQTVSKQAERLVTAFTNQTSYQTENDRLKEENAALKNKLLDYEDTKQQLTELEKFMGIKKEHADYVLSDPCSIIGYVTNDPFHSFIINKGSDDGIELQDPVVTAEGIVGIISSVSNTYSTVETICSPKLSIGAMSATGSDTGILEGEISLAADYQCRMIYLERDTKLQEGDLIKTSGTGGLFPQGYLIGSVDHLELMDSGLSKYAVLNPAVNFDALTSVMVILDYDGKGAETIPEDQTAVQTTVTTGTTTLAETTTETTTTVTVLTTETEAANGQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0U8</accession>
    <name>R5V0U8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">30S ribosomal protein S7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpsG</name>
        <name evidence="10" type="ORF">BN566_01624</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center, probably blocks exit of the E-site tRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 30S ribosomal subunit. Contacts proteins S9 and S11.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the universal ribosomal protein uS7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000171">
        <property type="protein sequence ID" value="CCZ83895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01624"/>
    <dbReference type="EnsemblBacteria" id="CCZ83895">
        <property type="protein sequence ID" value="CCZ83895"/>
        <property type="gene ID" value="BN566_01624"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.455.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00480_B">
        <property type="entry name" value="Ribosomal_S7_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000235">
        <property type="entry name" value="Ribosomal_S5/S7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005717">
        <property type="entry name" value="Ribosomal_S7_bac/org-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020606">
        <property type="entry name" value="Ribosomal_S7_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023798">
        <property type="entry name" value="Ribosomal_S7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036823">
        <property type="entry name" value="Ribosomal_S7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11205">
        <property type="entry name" value="PTHR11205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00177">
        <property type="entry name" value="Ribosomal_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002122">
        <property type="entry name" value="RPS7p_RPS7a_RPS5e_RPS7o"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47973">
        <property type="entry name" value="SSF47973"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01029">
        <property type="entry name" value="rpsG_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00052">
        <property type="entry name" value="RIBOSOMAL_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6 8" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="6" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Ribosomal_S7" evidence="9">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ83895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="156" mass="17928" checksum="C12E1586B0A7FDD9" modified="2013-07-24" version="1">MPRRGKIAKRDVLEDPIYKSKLVTRLINNVMVDGKKGVAQKIVYGAFDIVSEKTGKEALEMFEQAMENVMPVLEVKARRMGGATYQVPMEVRPERRQTLGLRWITKYSRLRNEKTMKERLAGEILDALNNTGGACKKRDDTHKMAEANKAFAHYRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>R5V197</accession>
    <name>R5V197_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphoribosyl-AMP cyclohydrolase</fullName>
            <shortName evidence="7">PRA-CH</shortName>
            <ecNumber evidence="7">3.5.4.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">hisI</name>
        <name evidence="9" type="ORF">BN566_01734</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the hydrolysis of the adenine ring of phosphoribosyl-AMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>1-(5-phospho-beta-D-ribosyl)-5'-AMP + H2O = 1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:20049"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58435"/>
            <dbReference type="ChEBI" id="CHEBI:59457"/>
            <dbReference type="EC" id="3.5.4.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="7">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 3/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the PRA-CH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.19" evidence="7"/>
    <dbReference type="EMBL" id="CBAR010000178">
        <property type="protein sequence ID" value="CCZ84005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01734"/>
    <dbReference type="EnsemblBacteria" id="CCZ84005">
        <property type="protein sequence ID" value="CCZ84005"/>
        <property type="gene ID" value="BN566_01734"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00008"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004635">
        <property type="term" value="F:phosphoribosyl-AMP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.810">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01021">
        <property type="entry name" value="HisI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026660">
        <property type="entry name" value="PRA-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002496">
        <property type="entry name" value="PRib_AMP_CycHydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038019">
        <property type="entry name" value="PRib_AMP_CycHydrolase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01502">
        <property type="entry name" value="PRA-CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141734">
        <property type="entry name" value="SSF141734"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="5 7 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PRA-CH" evidence="8">
        <location>
            <begin position="33"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ84005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="115" mass="13352" checksum="43AF8107D2011984" modified="2013-07-24" version="1">MIKIDCNHLDAYFEKGDLIPAIVYEVNTKTVLMLAYMNRESLKKTLETGYTWFWSRSRQELWNKGATSGHLQKVISMYSDCDNDTLLVNVEQTGVACHTGSYSCFFREMYEAEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1C2</accession>
    <name>R5V1C2_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">WYL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01761</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000181">
        <property type="protein sequence ID" value="CCZ84035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84035">
        <property type="protein sequence ID" value="CCZ84035"/>
        <property type="gene ID" value="BN566_01761"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026881">
        <property type="entry name" value="WYL_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13280">
        <property type="entry name" value="WYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WYL" evidence="1">
        <location>
            <begin position="144"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="323" mass="38513" checksum="317CFB69422EE0CF" modified="2013-07-24" version="1">MESAKNCCTLEIFYRALRGEWISVKKIAEEYQVSTKTIGRYINEIQQFLSENRELMQNAELLYSHKEKAYYLHADDFLENKELFALVKVVLGCRCFQKEDLLNLIAKLKRMTTPKDREQFENLIRKELYHYHEIKSDCNMVMDYLWKLIQAIEDKQILTITYYKMNREQVKRKIRPASILFSEYYFYLIAYDADDETCRAKYFRVDRIVSIVTHRERFSLKREYDFDEGDLREKNQFMFPGKTEKIRFSFSGLSVQAILDRLPTAKVVEKNGKESIIEAEVNHGRGIIMYLLSQGAWVKVLSPQSLVDEMREELQKMQSFYQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1H5</accession>
    <name>R5V1H5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01836</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000187">
        <property type="protein sequence ID" value="CCZ84110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01836"/>
    <dbReference type="EnsemblBacteria" id="CCZ84110">
        <property type="protein sequence ID" value="CCZ84110"/>
        <property type="gene ID" value="BN566_01836"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024523">
        <property type="entry name" value="DUF3793"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12672">
        <property type="entry name" value="DUF3793"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="190" mass="22070" checksum="33A337ABBAF60DE9" modified="2013-07-24" version="1">MSVSECVKFEQKLAYFMAPSLLGIKCASLMSINKTEFSVLEHLERFNARAHAKGLKLRLLCDCTNRALILLYQETLLQQQLQNPQVQLILRQQGYAEPVNLEAALQQLSERIRCSGEFPHEIGVFLGYPVEDVIGFMENKGKNYVLCKYWKVYHHAERAKHTFERYDRCRAFLCNQLNQGYDLYQALKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1I7</accession>
    <name>R5V1I7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_01851</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000189">
        <property type="protein sequence ID" value="CCZ84125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01851"/>
    <dbReference type="EnsemblBacteria" id="CCZ84125">
        <property type="protein sequence ID" value="CCZ84125"/>
        <property type="gene ID" value="BN566_01851"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00181"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016639">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006537">
        <property type="term" value="P:glutamate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028261">
        <property type="entry name" value="DPD_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006005">
        <property type="entry name" value="Glut_synth_ssu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14691">
        <property type="entry name" value="Fer4_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01317">
        <property type="entry name" value="GOGAT_sm_gam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0314">Glutamate biosynthesis</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fer4_20" evidence="6">
        <location>
            <begin position="23"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Pyr_redox_2" evidence="5">
        <location>
            <begin position="153"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF14691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ84125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="495" mass="55031" checksum="2EABAC7B9F9C5895" modified="2013-07-24" version="1">MGKATGFLEYERVENPGQEPLERIKTYREFHTPLNPEERQKQGARCMDCGVPFCQNGKPIMGMVSGCPLNNLCPEWNELVYKGRMEDAARRLLLTNCFPEFTSRVCPALCEKACTCGLYGDPVTVKENEYAIIESAYESGFMKPRIPKVRTGKKVAVIGSGPSGLAAALQLNRRGHSVTVYEREDRVGGLLMYGIPNMKLEKTVIDRRIALMEQEGVVFRTGVDVGKDISAEELQKQYDAVVLACGSSNPRNINAPGRESKGIYYAVEFLKATTKSLINSNLSDGYYISAKYKNVIVIGGGDTGNDCVGTAIRHGCKSVIQLEMMPKLPDQRAENNPWPEWPRICKTDYGQEEAIAVFGHDPRIYQTTVKRFIPNEEGHLKAVETIKLQSVRDEKTGRMRMEEIPGSEQILEADLVLIAAGFLGAQSYVADAFGVNLNARTNVATETPDSYQTNVEKVFTAGDMHRGQSLVVWAIREGREAARAVDEYLMHYSNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1K9</accession>
    <name>R5V1K9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Endoglucanase</fullName>
            <ecNumber evidence="10">3.2.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN566_01880</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>Endohydrolysis of (1-&gt;4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;4)-beta-D-glucosidic linkages in beta-D-glucans containing (1-&gt;3)- and (1-&gt;4)-bonds.</text>
            <dbReference type="EC" id="3.2.1.73"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8 10">Belongs to the glycosyl hydrolase 9 (cellulase E) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.4" evidence="10"/>
    <dbReference type="EMBL" id="CBAR010000193">
        <property type="protein sequence ID" value="CCZ84155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01880"/>
    <dbReference type="EnsemblBacteria" id="CCZ84155">
        <property type="protein sequence ID" value="CCZ84155"/>
        <property type="gene ID" value="BN566_01880"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110165">
        <property type="term" value="C:cellular anatomical entity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008810">
        <property type="term" value="F:cellulase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042972">
        <property type="term" value="F:licheninase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02850">
        <property type="entry name" value="E_set_Cellulase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008264">
        <property type="entry name" value="Beta_glucanase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004197">
        <property type="entry name" value="Cellulase_Ig-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003305">
        <property type="entry name" value="CenC_carb-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002105">
        <property type="entry name" value="Dockerin_1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="GH16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008263">
        <property type="entry name" value="GH16_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001701">
        <property type="entry name" value="Glyco_hydro_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033126">
        <property type="entry name" value="Glyco_hydro_9_Asp/Glu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018221">
        <property type="entry name" value="Glyco_hydro_9_His_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02018">
        <property type="entry name" value="CBM_4_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02927">
        <property type="entry name" value="CelD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00404">
        <property type="entry name" value="Dockerin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00722">
        <property type="entry name" value="Glyco_hydro_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00759">
        <property type="entry name" value="Glyco_hydro_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00737">
        <property type="entry name" value="GLHYDRLASE16"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="SSF48208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="SSF49899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="SSF81296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01034">
        <property type="entry name" value="GH16_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00592">
        <property type="entry name" value="GH9_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00698">
        <property type="entry name" value="GH9_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8 10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4 10" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="6 8 10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 8 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8 10" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005145538" description="Endoglucanase" evidence="10">
        <location>
            <begin position="34"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="13">
        <location>
            <begin position="720"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="12">
        <location>
            <begin position="795"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="781"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="646"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="689"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="698"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CCZ84155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="1021" mass="112043" checksum="E6B1DF7FAB5E18CC" modified="2013-07-24" version="1">MHRQIFQKSIAAIAAGAIAATSFAVLPSFSASAATTILSSDFSNGSSGWSTYKASGGSCSMGVENGKLALTVNSVGTLNYSVQVGYDVVPLYQNGVYRLKYDISSTEDCTVEQMIQQNGGTYQSYTWKGLDLTAETQTVDYTFTMKQETDIMSKLVFNCGYEGKDVAPHTIYLDNVSLELIDDSKVDYTSFQPYEPSIITDQVGYQPNSKKTAVFRDVTSETTFSVVNADTKQTVYTGTLSDSINNYPAKETEWTGDFSAVTEPGSYYITCGDLDQSYTFTISDDVYDTLLTDSVKMLYLQRCGTEIEDDTFGHVACHNTKATIYGTSETIDVNGGWHDAGDYGRYVVPGAKTVADLLYAYAASPSSYGDATGIPESGNGVPDILDETRYELEWMLKMQDAKSGGVHHKVTCENFPGYVMPENETQPLIVTPISTTATADFCASMAMASEFYQNVDKDFANTCLAAAKKAWDFLEENPKLIYENPEDISTGAYEDTSDTDERYWAAMQMYRATGDESYLQNFIGSAAKTGMDWSTVGDYGNIAILTMANPNQTMYAKAKKAVLKEADQFVSTAKSSPYGVSVVNFNWGSNMTVANAGVILGLAYQLTDDTSYLNVSASNLHYLLGSNPIGECFVTGFGTVSPQNPHHRPSMAKKQAMHGMVVGGVNSNLEDSAAKAYLATTAPAKCYVDHSESYSTNEIAIYWNSPLTYLISLNNANTGNTGTVGDVNQDGAVNVADVILLQKYLIRKATLTAEQGVNADMTKDNIVNVVDLCLLKKNVLKNNSNPSQPDQPTKPDQPSTNKPDPNATMYANFRAGSTEEFIASDGWTNGNPFDCFWKASNATFKDNALNLTIDKDPTGKYHYIGAEYRTNDFYSYGYYETSMKAIKNDGVVSSFFTYTGPSDNNPWDEIDVEVLGKDTTKVQFNYYTNGVGNHEYMYDLGFDASEGYHTYGFDWQKDSITWYVDGKAVYKATSNIPSTPGKIMMNVWPGIGVDDWLKPFDGTTPLTAKYQWVTYNENGHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1M1</accession>
    <name>R5V1M1_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ArAE_1_C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01895</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000193">
        <property type="protein sequence ID" value="CCZ84170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01895"/>
    <dbReference type="EnsemblBacteria" id="CCZ84170">
        <property type="protein sequence ID" value="CCZ84170"/>
        <property type="gene ID" value="BN566_01895"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010343">
        <property type="entry name" value="ArAE_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021062">
        <property type="entry name" value="ArAE_1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038323">
        <property type="entry name" value="ArAE_1_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06081">
        <property type="entry name" value="ArAE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11728">
        <property type="entry name" value="ArAE_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="ArAE_1_C" evidence="2">
        <location>
            <begin position="154"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="342" mass="39445" checksum="AE593764F1D4E488" modified="2013-07-24" version="1">MNLTFLKKIPWLKILKMTVGAVLAMELAGLLHLEYAASAGVIAFLTIQDTRQETSSSALRRGIAFCIMTLLCLPIFSLLGNRPWAFGIFFPLFLLICFTCRMEDSIAVNTVLATHYMAAGGVSLPMLKNECGLLLVGVGLGILLNSFMPNNLRRIQAKQSELDTELQQILHRMAIHVLKTEHPDYDGSCFHRTDQLLTEMEQETIRFMRNHYLEQDLYFMQYLMMRREQCEILRMIYRQICKLTLIPTQAKPLSDYLQTISDQYHETNDAVALLEELDALHRFYQEQPLPETREQFENRAILYAILSDLRSLLQIKQQFSVSLPKKGRGSSSFYRKQNFKTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1M5</accession>
    <name>R5V1M5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00357</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000236">
        <property type="protein sequence ID" value="CCZ84458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84458">
        <property type="protein sequence ID" value="CCZ84458"/>
        <property type="gene ID" value="BN566_00357"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="281"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="327" mass="37053" checksum="06475ACA16984875" modified="2013-07-24" version="1">MKKQLLHTYRFVFLCMLIVVVGLCLVAGYSVRVLDQNQVIQQVDTNMEAAKVRLDRDAEASATMMEDLRNEYASKTRVVAMLLEEQKDWSSENETILEELRVVIDADQISVSNETGILIASTDFSSTGKRAYRAFLSHTTDPVFTDVVFTMQNDKPMILAASALGSGKGLIQVQYPAESMLTQSQENDVSAIVADMPFSDMGISAIIDTDTGKYVSHTQVERCGTDSDYDESLFSGKKGKVDLLRQHQRYMIRYQTHENYILLVDIPYREMDNARGMVIKWVFASGLILLLVATLSMRMGYFYLKKKSPELFPKEPSDWAEQQKQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5V266</accession>
    <name>R5V266_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Chitobiose phosphorylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00317</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000224">
        <property type="protein sequence ID" value="CCZ84410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00317"/>
    <dbReference type="EnsemblBacteria" id="CCZ84410">
        <property type="protein sequence ID" value="CCZ84410"/>
        <property type="gene ID" value="BN566_00317"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11755">
        <property type="entry name" value="GH94N_ChBP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009342">
        <property type="entry name" value="Carb-bd_put_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033432">
        <property type="entry name" value="GH36_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037828">
        <property type="entry name" value="GH94N_ChBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037018">
        <property type="entry name" value="Glyco_hydro_65_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010383">
        <property type="entry name" value="Glyco_hydrolase_94"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17167">
        <property type="entry name" value="Glyco_hydro_36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06165">
        <property type="entry name" value="Glyco_transf_36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01068">
        <property type="entry name" value="CBM_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="SSF48208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="SSF74650"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CBM_X" evidence="2">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="794" mass="88800" checksum="F4B91CF750BD153A" modified="2013-07-24" version="1">MQYGYFDLANKEYVITRPDTPSAWANYLGSPEYGAIISNNAGGYSFVKSGANGRITRYRFNSNMALPGRYIYLRDNDANDYWSASWQPVGKPLDTYKSTCRHGTAYTVISAEYSGIRSDVTYYVPDGATHEVWRAIITNNSDRPRNLSAFGFVEFTNEDNYEQDQVNLQYSLFISRTAFEQNKMMQYISEYSEKDATSRFFGMVGAPITAYNGSLEHFIGSYRTYSNPIAVESGHCDNELNYNSNSCGALQTELTLQPGESKELIFVLGRKTSAEADEILHTYETAGKVDAEVAALKQFWHEKLNHFSVETPSEAFNNMINVWNAYQCFITFIWSRAASFIYCGLRNGYGYRDTVQDIQGIIHLDPELAAEKIRFMLSAQVDNGGGLPLVKFNHNAGHENTPDDPEYVKETGHPSYRADDALWLFPTIAKYVGESGNAAFYDEVIVYANGGEDTVYDHLKRAIQFSMERLGDHSMPAGLHADWNDCLRLGKKGESTFVAFQLYYAMTMMKKLAAERNDAVYVDYLEDAQAKLAQTLQTCWDEDRFIRGIREDGVIVGAKKDPEANMWLNPQSWSVISGFASKEQADTAMQSVADILDTPYGAKLLEPPYRHHYFDGALMHIFNPDTKENGGIFSQSQGWLILAEALLGHGNAAFRYFTESSPAAMNDKAEIRVMEPYVHGQFTESSASPYAGRAHVHWLTGTASTVMVGCVEGICGMRPDADGLAIRPSIPAEWDGMKMHKVFRGKQLNIIVQNPNHVESGVCALTLNGEALQGDYVPAEKLQDTNEIVVTLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>R5V291</accession>
    <name>R5V291_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 9 11">Uracil-DNA glycosylase</fullName>
            <shortName evidence="9">UDG</shortName>
            <ecNumber evidence="4 9 11">3.2.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ung</name>
        <name evidence="13" type="ORF">BN566_00342</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 9 11">Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9 11">
            <text>Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil.</text>
            <dbReference type="EC" id="3.2.2.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9 11">Belongs to the uracil-DNA glycosylase (UDG) superfamily. UNG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.27" evidence="4 9 11"/>
    <dbReference type="EMBL" id="CBAR010000225">
        <property type="protein sequence ID" value="CCZ84435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00342"/>
    <dbReference type="EnsemblBacteria" id="CCZ84435">
        <property type="protein sequence ID" value="CCZ84435"/>
        <property type="gene ID" value="BN566_00342"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004844">
        <property type="term" value="F:uracil DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd10027">
        <property type="entry name" value="UDG-F1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00148">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002043">
        <property type="entry name" value="UDG_fam1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018085">
        <property type="entry name" value="Ura-DNA_Glyclase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005122">
        <property type="entry name" value="Uracil-DNA_glycosylase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11264">
        <property type="entry name" value="PTHR11264"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03167">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00986">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="SSF52141"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00628">
        <property type="entry name" value="ung"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00130">
        <property type="entry name" value="U_DNA_GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9 11" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8 9 11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7 9 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDG" evidence="12">
        <location>
            <begin position="49"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9 10">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CCZ84435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="225" mass="25538" checksum="27B4BFDC665428BA" modified="2013-07-24" version="1">MVHIGNDWDDLLQDAIQSESYQHLRQFLIQEYRTHTIYPNMYDIFNAFQYTPYADVKVVILGQDPYHNPNQAHGLSFSVHKGVTPPPSLVNIFKELRDDTGIDNLGKHGDLTEWAKHGVLLLNTVLTVRAGAANSHRGKGWEQLTDTVIDKLNEREKPVVFLLWGNNAKAKQQRITAPQHLVLTAAHPSPLSAHYGFFGCRHFSKTNAFLIEHGIEPVDWRIDPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>R5V2M4</accession>
    <name>R5V2M4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">30S ribosomal protein S17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rpsQ</name>
        <name evidence="6" type="ORF">BN566_00066</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">One of the primary rRNA binding proteins, it binds specifically to the 5'-end of 16S ribosomal RNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3 4">Belongs to the universal ribosomal protein uS17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000241">
        <property type="protein sequence ID" value="CCZ84520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00066"/>
    <dbReference type="EnsemblBacteria" id="CCZ84520">
        <property type="protein sequence ID" value="CCZ84520"/>
        <property type="gene ID" value="BN566_00066"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01345_B">
        <property type="entry name" value="Ribosomal_S17_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019984">
        <property type="entry name" value="Ribosomal_S17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000266">
        <property type="entry name" value="Ribosomal_S17/S11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019979">
        <property type="entry name" value="Ribosomal_S17_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744">
        <property type="entry name" value="PTHR10744"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00366">
        <property type="entry name" value="Ribosomal_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00973">
        <property type="entry name" value="RIBOSOMALS17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03635">
        <property type="entry name" value="uS17_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00056">
        <property type="entry name" value="RIBOSOMAL_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 4 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 3 5" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ84520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="85" mass="9923" checksum="85838D8F5B5C96FC" modified="2013-07-24" version="1">MAERNLRKTRVGRVVSDKMDKTVVVAIVDNVKHPLYKKIVKRTVRLKAHDENNACKVGDRVEVMETRPLSKDKRWRVVEIIEKAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5V2P8</accession>
    <name>R5V2P8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Leucine-rich repeat (LRR) protein-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00406</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000242">
        <property type="protein sequence ID" value="CCZ84545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00406"/>
    <dbReference type="EnsemblBacteria" id="CCZ84545">
        <property type="protein sequence ID" value="CCZ84545"/>
        <property type="gene ID" value="BN566_00406"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026906">
        <property type="entry name" value="LRR_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13306">
        <property type="entry name" value="LRR_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004394752" evidence="2">
        <location>
            <begin position="29"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="226" mass="23962" checksum="8E47C2D96EDBBD73" modified="2013-07-24" version="1">MMQQWKRSMWAALLTLATLFSFTGCSSSDDESSESSVDLSNIASAMITEGTTEALTTDYAYIMNDDGTISVSGYTGSDTDLTVPGLIDGYVVTGIADHAFEANADIKSITLPNGLIAIGESAFSDCSSLTTVVIPDTVGTIRRGAFISCTALTTLDIPASVTEIMEEAFTGCAALKDLTVESSTVEFTNWWVDTDEIPSDLTITCPKDSKIAAWAEKNNVATEPLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>R5V2Y2</accession>
    <name>R5V2Y2_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN566_00488</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CBAR010000257">
        <property type="protein sequence ID" value="CCZ84635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00488"/>
    <dbReference type="EnsemblBacteria" id="CCZ84635">
        <property type="protein sequence ID" value="CCZ84635"/>
        <property type="gene ID" value="BN566_00488"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006468">
        <property type="term" value="P:protein phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005543">
        <property type="entry name" value="PASTA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03793">
        <property type="entry name" value="PASTA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00740">
        <property type="entry name" value="PASTA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51178">
        <property type="entry name" value="PASTA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="346"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="13"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="PASTA" evidence="10">
        <location>
            <begin position="377"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="PASTA" evidence="10">
        <location>
            <begin position="447"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="domain" description="PASTA" evidence="10">
        <location>
            <begin position="519"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="299"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="686"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="300"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="320"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ84635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="709" mass="78458" checksum="95594DC06DDFB16A" modified="2013-07-24" version="1">MDNYIGKRLDGRYEITELIGQGGMANVYKATDVLDNRIVAVKILKREFSESDEFLRRFRNESKAIAVLSHPNIVKIYDMGFSEKLQYIVMEYIDGITLKEYIDSERVLNWKDAVHFIIQILRALQHAHNRGIVHRDIKPQNIMLLTDGTIKVMDFGIAKFAREESMTATDQAIGTVHYISPEQARGDVTDEKSDIYSVGVMFYEMLTGQKPFDTDNPVSIAVMHIKNIPVRPRDINPNIPSGLEEIIMHAMEKDAENRYQTASDMIRDIEAFKQNNQIIFGYYSVPLVQQRAAENGRMQYGMPPQQSVAPMQPQQQPEYLPEEPDEDEDDYEEPDEYEPEPKQKSLVVPILTAVTVVVIVVAAVFIVALLTNPNLLRKSVEVPNLIGMTYDDAVKNYDKDIVLSVEEEDYNDDYAAGTIYNQSEDAGTRVDRGDNNKIRVAVAVSKGTQMISVPEVKGYSEAEAKNMLTDLGFQVHEIQEASSSDDGIEKGNCIRTDPAYGTELKVGSNIDLYVSTGIAVDEITVPQFVGMTQADAETECARINLKIASVEEVDSDKPQGEIVEQSLKAYSATNAGSDISFKISNGSAPEGEINYGMYLPSDITGKYTVDFTMDGKVIQSYPFVAETAGGMISIPVKGTGQKQVTVSVTGESTGMTARLGVYTFDFTTGSFTAISEDFESAFAAVTPQHTEAAPAATEAPAAQSEESQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>R5V3W6</accession>
    <name>R5V3W6_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Phenylalanine--tRNA ligase beta subunit</fullName>
            <ecNumber evidence="14">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Phenylalanyl-tRNA synthetase beta subunit</fullName>
            <shortName evidence="14">PheRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">pheT</name>
        <name evidence="19" type="ORF">BN566_00604</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + H(+) + L-phenylalanyl-tRNA(Phe)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="14">Binds 2 magnesium ions per tetramer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 14">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="14"/>
    <dbReference type="EMBL" id="CBAR010000008">
        <property type="protein sequence ID" value="CCZ82799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00604"/>
    <dbReference type="EnsemblBacteria" id="CCZ82799">
        <property type="protein sequence ID" value="CCZ82799"/>
        <property type="gene ID" value="BN566_00604"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00769">
        <property type="entry name" value="PheRS_beta_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02796">
        <property type="entry name" value="tRNA_bind_bactPheRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00283">
        <property type="entry name" value="Phe_tRNA_synth_beta1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005146">
        <property type="entry name" value="B3/B4_tRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005121">
        <property type="entry name" value="Fdx_antiC-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036690">
        <property type="entry name" value="Fdx_antiC-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045060">
        <property type="entry name" value="Phe-tRNA-ligase_IIc_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004532">
        <property type="entry name" value="Phe-tRNA-ligase_IIc_bsu_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020825">
        <property type="entry name" value="Phe-tRNA_synthase-like_B3/B4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041616">
        <property type="entry name" value="PheRS_beta_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002547">
        <property type="entry name" value="tRNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033714">
        <property type="entry name" value="tRNA_bind_bactPheRS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005147">
        <property type="entry name" value="tRNA_synthase_B5-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947">
        <property type="entry name" value="PTHR10947"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03483">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03484">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03147">
        <property type="entry name" value="FDX-ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01588">
        <property type="entry name" value="tRNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17759">
        <property type="entry name" value="tRNA_synthFbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00873">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00874">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00896">
        <property type="entry name" value="FDX-ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54991">
        <property type="entry name" value="SSF54991"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00472">
        <property type="entry name" value="pheT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51483">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51447">
        <property type="entry name" value="FDX_ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50886">
        <property type="entry name" value="TRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 14" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 14 19" id="KW-0436">Ligase</keyword>
    <keyword evidence="10 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12 14" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 15" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 15" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="TRNA-binding" evidence="16">
        <location>
            <begin position="39"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="B5" evidence="18">
        <location>
            <begin position="408"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="FDX-ACB" evidence="17">
        <location>
            <begin position="704"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="457"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="463"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="466"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="467"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CCZ82799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="797" mass="87527" checksum="12A25A644BD90A4B" modified="2013-07-24" version="1">MNLSMKWLSDYVTLDTTVKDFCAAMTMSGSKVEVATEEGSEIKNVVVGKLLSVVPHENSDHLVVCQVDVGQEQPIQIVTGAPNVKAGDIVPVALCGAELPNGVKIKKGKLRGVESNGMLCSLGELGLTVHDFPYAIADGIFLIQEDCQIGQDIHEAIGLNDTSVEFEITSNRPDCLSVTGLAREAAATYHVPLNLKKPTFQGIDGNIQDALQVEIQNKEKCPRYAAGIVKNVKIAPSPRWMRERLRASGVRPINNLVDITNYVMLEYGQPMHAFDLRYVKDGKIVVRNAAEGETITTLDGVTRTLSPEMLVIADAEKPIAVAGVMGGEYSGIMEDTNTVVFESAYFEPVQVRRTAKKLGMRTDASARYEKGLDPEGCLRTLERAFELVELLGAGEPVRTHIDLNYNQKQRNRIPFDPDWINKFLGTDISREEMCDTMKMLEIEVDGDTCISPSFRIDLERPADLAEEVARIYGYNNIPSTVIKGIANASLTPKQKFRRTLENATVAVGCYGILTYSFISPKYFDKIALPADSSLRKTVVISNPLGEDTSVMRTTTLPSMLETLSLNYKNRNAAVALYEIGKEYLPTAPDKLPEEPDRLTIGMYGDDADFFTLKGMVETILETAGLHDCTYKACGTDAPFDEICALHPGRSAVIYAGETPIGYLGEVHPTVQKNYDIGTRTYVAKLLIDEMQPLAQTEITYQPLPKFPAITRDLSLVCADEVPVGDLQAAMKNAVGNILEQITLFDVYKGEQIAAGMKSVSFSIRMRSHEGTLTDEQADAAMKRVLKALKEHGATLRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>R5V3Z2</accession>
    <name>R5V3Z2_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glycerol-3-phosphate acyltransferase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Acyl-PO4 G3P acyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Acyl-phosphate--glycerol-3-phosphate acyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">G3P acyltransferase</fullName>
            <shortName evidence="10">GPAT</shortName>
            <ecNumber evidence="10">2.3.1.275</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Lysophosphatidic acid synthase</fullName>
            <shortName evidence="10">LPA synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">plsY</name>
        <name evidence="11" type="ORF">BN566_00639</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the transfer of an acyl group from acyl-phosphate (acyl-PO(4)) to glycerol-3-phosphate (G3P) to form lysophosphatidic acid (LPA). This enzyme utilizes acyl-phosphate as fatty acyl donor, but not acyl-CoA or acyl-ACP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>an acyl phosphate + sn-glycerol 3-phosphate = a 1-acyl-sn-glycero-3-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:34075"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:57970"/>
            <dbReference type="ChEBI" id="CHEBI:59918"/>
            <dbReference type="EC" id="2.3.1.275"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="10">Lipid metabolism; phospholipid metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Probably interacts with PlsX.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the PlsY family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.275" evidence="10"/>
    <dbReference type="EMBL" id="CBAR010000012">
        <property type="protein sequence ID" value="CCZ82834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00639"/>
    <dbReference type="EnsemblBacteria" id="CCZ82834">
        <property type="protein sequence ID" value="CCZ82834"/>
        <property type="gene ID" value="BN566_00639"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00085"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043772">
        <property type="term" value="F:acyl-phosphate glycerol-3-phosphate acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01043">
        <property type="entry name" value="PlsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003811">
        <property type="entry name" value="G3P_acylTferase_PlsY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30309">
        <property type="entry name" value="PTHR30309"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02660">
        <property type="entry name" value="G3P_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01207">
        <property type="entry name" value="G3P_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00023">
        <property type="entry name" value="TIGR00023"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="1 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2 10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 10" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="9 10" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="120"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ82834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="229" mass="24905" checksum="8A0639971ABC4C86" modified="2013-07-24" version="1">MLVIAILLTAVVSYLLGSCNSAIIVGKLIQKKDIRDYGSHNAGLTNTLRCFGKGCAALTLIGDLGKGIVAVLLSKGIFHLLQVGFTDAQDLQLVGYIAGIFAILGHVFPLYYGFKGGKGVLVGVSVFLVIDWRVFCILIGVFAIVLILSKYVSLSSMIAAGCCPIVTFLMQHFRAEPLPLWYQLVNTAMAALMGGWVIFMHRSNMERLKNGTENRFSFHRSADTTKPKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5V407</accession>
    <name>R5V407_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00654</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000012">
        <property type="protein sequence ID" value="CCZ82849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00654"/>
    <dbReference type="EnsemblBacteria" id="CCZ82849">
        <property type="protein sequence ID" value="CCZ82849"/>
        <property type="gene ID" value="BN566_00654"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="101"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="153"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="183"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="206"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="229"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="254"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="287"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="321"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="348"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="371"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="394"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="421"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="445"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="475"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="527"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="554"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="579"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="605"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="629"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ82849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="669" mass="75133" checksum="E3BF65F548EB4D0E" modified="2013-07-24" version="1">MSPEEEKVQRLEQRIAQLEQALQEKNAPASLQPTVSPTIPQQETPPTIPPPAPEIPPMQTTQPTPPPPSFQPNIPWTSQAQTMQSVPPPQQMDTEQKFGKIVMGILASIFIFAAILIGYAFLPSILQMVGLFVIGAGVSAAGLYGISKKKYPYFFLSVAGCGAGILYLSILLSYWYFSVVGKWAMYGMLLLWAVFVLWMSRKSVPLFRLIGQCGIVLSLLFAFRQLNVLETGLIFFFFVLVSALYQFADRRIGIACQLSTLLCNAVSLLILTVLFTVTLRRVLHTPMFGLLLAEGILLMLYTLAQLIWYRMRMRPAEQSGMGYFFTALSGLLLVQLQAQWFFFFWDTGKYLLSALTAVAVWMLYDRKQEESALRNLMACICSILAFLWLLPLSILPALLGWVLLEAALFVMWHVRKQNGCLYLGYVLFLFGIRLILSKISVLDDITLQYGAWVAIDIAVTLYLRHCRSHRGMEILSLCVTGWLMVEGWCLLLLEGLFRSVAAFWHAIGSLFRQTGSDTAIVIPWDTILLAAFVVLIFLLNVPYLLRKYPNRNGVSVYLGIRMTLLGAVVSGAFFQRAAWSSGLLTLLSFGMLLVGFHFHQKGLRIYSLIAMMVFLCKLLLLDLSYQTPLLWAGAFLICGLLCFGISFAYNRIGQKHIESAEPMKQKGEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5V4L8</accession>
    <name>R5V4L8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CttA_N domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00817</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000040">
        <property type="protein sequence ID" value="CCZ83024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00817"/>
    <dbReference type="EnsemblBacteria" id="CCZ83024">
        <property type="protein sequence ID" value="CCZ83024"/>
        <property type="gene ID" value="BN566_00817"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040959">
        <property type="entry name" value="CttA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18244">
        <property type="entry name" value="CttA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CttA_N" evidence="2">
        <location>
            <begin position="287"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="481" mass="50514" checksum="11BF6755BFD6C35B" modified="2013-07-24" version="1">MDNAAQLRFAFASNDVNNTNNAKTADGSAIGELVYDIPDAETVKSIADQYGISLVTGTDDEGNEVSYYEFPLTWSEAVGEHGETATQCGSYVDGALVEIPYDQYTRRDGTICVVVPSETTTTAATTTTAEVTTTTEAVTTAAVDTTTEVPATTTTAAATTTTEAATTTTEAATTTTEAATTTTEAATTTTEAVTTTTEAATTTTEAATTTTEAATTTTEAVTTTTEAATTTTEAATTTTEAATTTTEAATTTTEATTTSTTEATTTTSATTTQPQPNVTTIVFELADPNFYFSVDEREFKATDLFKSVTLISTDADGKIVSQEDIIDKVNFNGATPKSTYVQADKYYAGTIQAYYNNGTENVLIPDATPTVYIGVKGDADLNGLEDVPDAVSILTYYAKIAAGQQGIVFNEDPMLNKLAYFLADVDTESKAGENTDGKLMEVNDAVYVLTYYAKKAAGQGPTWPDVIPSLKSLEGSMWYEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5V4Q1</accession>
    <name>R5V4Q1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00862</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000051">
        <property type="protein sequence ID" value="CCZ83074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83074">
        <property type="protein sequence ID" value="CCZ83074"/>
        <property type="gene ID" value="BN566_00862"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017259">
        <property type="entry name" value="UCP037672"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12650">
        <property type="entry name" value="DUF3784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="116" mass="12630" checksum="34DECC494257835F" modified="2013-07-24" version="1">MGDWIGFSIMLLFSIGCYVLSGFFLHGKGACLIAGYNTMSKKEQEQYDKDKLCRSTGYGMLIIGIALTLLSCVILLTEIIPVLPEWTLIAGNIGFCSIVALVTLGNLLFSNTKAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>R5V4U5</accession>
    <name>R5V4U5_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Carbamoyl-phosphate synthase pyrimidine-specific large chain</fullName>
            <ecNumber evidence="6">6.3.5.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Carbamoyl-phosphate synthetase ammonia chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">BN566_00916</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 ATP + H2O + hydrogencarbonate + L-glutamine = 2 ADP + carbamoyl phosphate + 2 H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:18633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the CarB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.5" evidence="6"/>
    <dbReference type="EMBL" id="CBAR010000062">
        <property type="protein sequence ID" value="CCZ83129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00916"/>
    <dbReference type="EnsemblBacteria" id="CCZ83129">
        <property type="protein sequence ID" value="CCZ83129"/>
        <property type="gene ID" value="BN566_00916"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00115"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01424">
        <property type="entry name" value="MGS_CPS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1030.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006275">
        <property type="entry name" value="CarbamoylP_synth_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005480">
        <property type="entry name" value="CarbamoylP_synth_lsu_oligo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036897">
        <property type="entry name" value="CarbamoylP_synth_lsu_oligo_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CbamoylP_synth_lsu-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005483">
        <property type="entry name" value="CbamoylP_synth_lsu_CPSase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011607">
        <property type="entry name" value="MGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036914">
        <property type="entry name" value="MGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033937">
        <property type="entry name" value="MGS_CPS_CarB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02787">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02142">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00098">
        <property type="entry name" value="CPSASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01096">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00851">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48108">
        <property type="entry name" value="SSF48108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52335">
        <property type="entry name" value="SSF52335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01369">
        <property type="entry name" value="CPSaseII_lrg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51855">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0436">Ligase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15" id="KW-0464">Manganese</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ATP-grasp" evidence="18">
        <location>
            <begin position="17"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="18">
        <location>
            <begin position="483"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="domain" description="MGS-like" evidence="19">
        <location>
            <begin position="742"/>
            <end position="877"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00014301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00030577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CCZ83129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="877" mass="97615" checksum="714AAE6B75A3C70E" modified="2013-07-24" version="1">MLRDICKNGLRLSPITQVLIEKCISGWKEIEFEVIRDRKGNVITVCSMENFDPVGIHTGDSIVIAPAVTLTDREYQMLRTAAINIISELKVEGGCNCQFALHPTSMQYAVIEVNPRVSRSSALASKATGYPIAKVATRIAIGYTLDEIINQVTGKTYACFEPALDYVVVKYPKWAFDKFVYAKRTLGTQMKATGEVMAIGTSFEQAIMKAVRSIELGVDSMNMKKYAKMSLDEIMEHLKVVDDERAFQVFEALKRGVTVEELHEKTMIDCWFLNKLLNLVHLEQWLADGTLTEQKYKLAKQYGYLDSTIERMSGQKCPMHQHAVYKMVDTCAGEFKAETPYFYSTYDEENEALQFMERTASGKKKVIVFGSGPIRIGQGIEFDYCSVHCVWTLKEMGYEAIICNNNPETVSTDFDTGDRLYFDPLTKEDVANIVQTEQPYGVVVQFGGQTAINLTRYLSDMGVKILGTTADDIDAAEDRERFDELLAKCNIPRPAGLTVMNTEEALAAAKQLGYPVLMRPSYVLGGQNMIIAHSDKDIVEYMAIIMSHMIENPVLIDKYMMGTEVEVDAICDGTDFLIPGIMEHVERAGIHSGDSISIYPAQNLNQKVTDTIVRYTGLLAKELHAVGLINIQYVVYNNEVYVIEVNPRSSRTVPYISKVTGIPMVDLATKIMLGETLKSLGYESGLYPSGDYVAVKVPVFSFEKLQDVDTMLGPEMKSTGECLGIGRTFEDALLKGLIAAGYDLKKEGGVLISVRDSDKQEIIPIADRFSRMGFELYGTSGTANVLNHNMIATNLVRKISEGEPNTITLLESGKIHYMISTSEKGRMPARDSVKMRRKSVERSICCLTAIDTASALSKILESGRSIDDVELVDITKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5V528</accession>
    <name>R5V528_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Tetracenomycin polyketide synthesis O-methyltransferase TcmP</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01010</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000080">
        <property type="protein sequence ID" value="CCZ83229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01010"/>
    <dbReference type="EnsemblBacteria" id="CCZ83229">
        <property type="protein sequence ID" value="CCZ83229"/>
        <property type="gene ID" value="BN566_01010"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007213">
        <property type="entry name" value="Ppm1/Ppm2/Tcmp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016874">
        <property type="entry name" value="TcmP-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04072">
        <property type="entry name" value="LCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028177">
        <property type="entry name" value="Polyketide_synth_Omtfrase_TcmP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="271" mass="30408" checksum="0E4DB579B2FE9A06" modified="2013-07-24" version="1">MGTEKIKPELNGVAETMLLTFYARAQYSKSKKHAFYDAKAVELVERLDYDFSSASKDVAMGGGTIARTLVFDELVRDFLQKHPDAVGVNIACGLDTRVYRMDTGVCTWYNLDLPETIAVRDPIFQEQGRISTIGCSVLDPAWADAVTKRGTMLFIIEGLTMYLSEADIAAMLKIIHDHFDNAYVMMETTSPFWVKKQSIEASVAATGAKFTWGANSFAEMESLAPGFVSVKDDNILRGMVQLYPIYRLVSWMPMIRKMAEKILIFQKKSFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5V5U9</accession>
    <name>R5V5U9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01263</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000117">
        <property type="protein sequence ID" value="CCZ83494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01263"/>
    <dbReference type="EnsemblBacteria" id="CCZ83494">
        <property type="protein sequence ID" value="CCZ83494"/>
        <property type="gene ID" value="BN566_01263"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013196">
        <property type="entry name" value="HTH_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028349">
        <property type="entry name" value="PafC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026881">
        <property type="entry name" value="WYL_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08279">
        <property type="entry name" value="HTH_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13280">
        <property type="entry name" value="WYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016838">
        <property type="entry name" value="PafC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH_11" evidence="1">
        <location>
            <begin position="6"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="WYL" evidence="2">
        <location>
            <begin position="132"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="307" mass="34877" checksum="3BBE7C5589D4A21A" modified="2013-07-24" version="1">MKIDRLIGILSVLLQREQVTAFELAEKFEVSHRTILRDMDALCCAGIPIVTEKGRNGGIRIMEGYKLDKTVLLQSEMQAILAGLQSLDSISGTHRYRQLMEKLSVSTAKTIPAEHPVLIDLSAWDSGAVSQKIEQIQAAMEQNCKISFSYFAPSGTTSREIEPYHLIFQWGHWYVWGYCDLRQDYRLFKLTRITDLQIEPEQRAARSVPAYTPDPMLPEEPAVPVLVRFSAAVKWRVMDDFGTEALQTEPDGSLLVSVCWQDLQALYWRLLAYGEQVEILEPPEARADFAEMLRKILKKYAETSGGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5V6A0</accession>
    <name>R5V6A0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01385</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000147">
        <property type="protein sequence ID" value="CCZ83649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83649">
        <property type="protein sequence ID" value="CCZ83649"/>
        <property type="gene ID" value="BN566_01385"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="52" mass="5648" checksum="3F0130975B9F071F" modified="2013-07-24" version="1">MNQDEQKPCCVNCHISGVPLYLGLDGKQHCADHIGLLLPDSPKQGTKQKGCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5V6C2</accession>
    <name>R5V6C2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phage capsid protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01410</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000148">
        <property type="protein sequence ID" value="CCZ83674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01410"/>
    <dbReference type="EnsemblBacteria" id="CCZ83674">
        <property type="protein sequence ID" value="CCZ83674"/>
        <property type="gene ID" value="BN566_01410"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024455">
        <property type="entry name" value="Phage_capsid"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05065">
        <property type="entry name" value="Phage_capsid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01554">
        <property type="entry name" value="major_cap_HK97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="4"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="462" mass="50086" checksum="0D290008A7D98185" modified="2013-07-24" version="1">MLKALLLRNKIDSKKAELAELRTAAAELEKREKELESDINEAKTEEEKAVVEKAVNQFEQDKAENEKSISELETEIADMEKELDAVEQKQQTPQIEGNSGDEIRKGKVKMEARVKFFGMNVQERDAFFANDAVKSWLERVREMGKNQRSITGAELLIPEVALDLIKETTLKYSKLYKHVNVKSVPGKARQNVMGAIPEAIWTEMCSTLNELNLTFNNVEVDGYKVGGFIAICNAVLEDSDIALATEIISALGQAIGYALDKAILYGTGTKMPLGIVTRLTQAAKPSGYSTTARAWANLTTSNVLAISGKTDAALFKELVIASGNAKADYSHGEMFWAMNEKTFTKLVANALTINAAGAIVTGQNGTMPVIGGAIEKLSFIPDDVIIGGYGDLYLLAERAGTAISQSEHARFIEDQTVFKGTARYDGLPVIAEGFVAIGIGGTKPTANAVTFAEDTANKVTGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5V6P7</accession>
    <name>R5V6P7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Dipeptidase putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_01529</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000162">
        <property type="protein sequence ID" value="CCZ83794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01529"/>
    <dbReference type="EnsemblBacteria" id="CCZ83794">
        <property type="protein sequence ID" value="CCZ83794"/>
        <property type="gene ID" value="BN566_01529"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016805">
        <property type="term" value="F:dipeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010964">
        <property type="entry name" value="M20A_pepV-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="SSF55031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01887">
        <property type="entry name" value="dipeptidaselike"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00758">
        <property type="entry name" value="ARGE_DAPE_CPG2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00759">
        <property type="entry name" value="ARGE_DAPE_CPG2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0224">Dipeptidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="M20_dimer" evidence="4">
        <location>
            <begin position="245"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="449" mass="48678" checksum="FCAF9A5CD683353D" modified="2013-07-24" version="1">MLPEFVKQKIEENKSAMLDTLQTLIGIPSVATAEPENGTPYGTNCAKALDFMLQTAKSFGLHTTNFDYHVGVADTSTDTAPYLGILAHLDVVPVMEENWHTPPFTATIQNDCIYGRGAIDDKGPAVAALYAIKAIADAGIQLKKPVRLLLGCNEENGSTDLEYYNQKDTMPPLVFTPDGSYPLISIEKGMMRIQFEAPATKDSILLLKAGTAPNAVPAQAVCVLPALYHKLLPENCNIIANSDFVKLTWNGQAAHASTPETGNNAITGLLELLAKLPDFPMCKALSACFPHGCTDGSNLGIACEDAESGALTCICSQLEIQGDKWVGMIDIRYPISTTKEAIFQQVQKRLQETGCSCKIIIQNDPHVVPKESPFVQTLLRIYEEETGEKGECIAIGGGTYVHDIPGGVAFGAEFPNWDYHMHGDDEFVPIEHLMKDVRMMAAAILELCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5V710</accession>
    <name>R5V710_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01618</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000169">
        <property type="protein sequence ID" value="CCZ83889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83889">
        <property type="protein sequence ID" value="CCZ83889"/>
        <property type="gene ID" value="BN566_01618"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="34" mass="3989" checksum="7F0F8ECF80B241F6" modified="2013-07-24" version="1">MVDIFVAFCYTKNGRIADMGLLPVSMQWMTEKEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>R5V723</accession>
    <name>R5V723_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal RNA small subunit methyltransferase H</fullName>
            <ecNumber evidence="6">2.1.1.199</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">16S rRNA m(4)C1402 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">rRNA (cytosine-N(4)-)-methyltransferase RsmH</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rsmH</name>
        <name evidence="7" type="ORF">BN566_01628</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>cytidine(1402) in 16S rRNA + S-adenosyl-L-methionine = H(+) + N(4)-methylcytidine(1402) in 16S rRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42928"/>
            <dbReference type="Rhea" id="RHEA-COMP:10286"/>
            <dbReference type="Rhea" id="RHEA-COMP:10287"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74506"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.199"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the methyltransferase superfamily. RsmH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.199" evidence="6"/>
    <dbReference type="EMBL" id="CBAR010000171">
        <property type="protein sequence ID" value="CCZ83899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01628"/>
    <dbReference type="EnsemblBacteria" id="CCZ83899">
        <property type="protein sequence ID" value="CCZ83899"/>
        <property type="gene ID" value="BN566_01628"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071424">
        <property type="term" value="F:rRNA (cytosine-N4-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01007">
        <property type="entry name" value="16SrRNA_methyltr_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002903">
        <property type="entry name" value="RsmH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023397">
        <property type="entry name" value="SAM-dep_MeTrfase_MraW_recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11265">
        <property type="entry name" value="PTHR11265"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01795">
        <property type="entry name" value="Methyltransf_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004486">
        <property type="entry name" value="MraW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81799">
        <property type="entry name" value="SSF81799"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00006">
        <property type="entry name" value="TIGR00006"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 6 7" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="6">
        <location>
            <begin position="33"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ83899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="315" mass="34715" checksum="903575880069386C" modified="2013-07-24" version="1">MTFQHVPVLLQETLSGLNIQPEGIYVDGTVGGAGHACEIAKQLHGAGRLIGLDRDPDAVAVATQRLAPYPATVIHANYDEMAQVLQRLEIPAVDGILLDLGVSSHQLDTEERGFSYHTDAPLDMRMSQSGMSAADVVNTWSQEELTRILFAYGEESFARAIARNICQARQEQPITTTLQLAEIVKSSVPQRVRRAKNPCKQTFQAIRIAVNDEFGHLERALTAAFSCLKPGGRLAIITFHSLEDRMVKQQFAKWCQGCTCPPDFPKCVCGKVPQGKLVNRKPIVADAAELEENRRSRSAKLRVIERSAAIDESNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>R5V752</accession>
    <name>R5V752_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Indole-3-glycerol phosphate synthase</fullName>
            <shortName evidence="11">IGPS</shortName>
            <ecNumber evidence="4 11">4.1.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">trpC</name>
        <name evidence="13" type="ORF">BN566_01658</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate + H(+) = (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:23476"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58613"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="EC" id="4.1.1.48"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 4/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the TrpC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.48" evidence="4 11"/>
    <dbReference type="EMBL" id="CBAR010000173">
        <property type="protein sequence ID" value="CCZ83929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01658"/>
    <dbReference type="EnsemblBacteria" id="CCZ83929">
        <property type="protein sequence ID" value="CCZ83929"/>
        <property type="gene ID" value="BN566_01658"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00043"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004425">
        <property type="term" value="F:indole-3-glycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00331">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00134_B">
        <property type="entry name" value="IGPS_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045186">
        <property type="entry name" value="Indole-3-glycerol_P_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013798">
        <property type="entry name" value="Indole-3-glycerol_P_synth_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001468">
        <property type="entry name" value="Indole-3-GlycerolPSynthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22854">
        <property type="entry name" value="PTHR22854"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00218">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00614">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9 11" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="7 11" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="10 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="IGPS" evidence="12">
        <location>
            <begin position="3"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CCZ83929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="259" mass="28989" checksum="0FD27740A347813D" modified="2013-07-24" version="1">MILDEIVGKRKEQLARELQRTDRETMRKLALRTEREVYSFPDALKTGRLSIIAEVKKASPSKSVICADFHPVEQAAAYERAGADAISCLTEEHYFQGSSDYFRAIREAVSIPMLRKDFIIDTYQIDEARVMGADAILLIAAILDAPTMRHFYEYATELGLHCLFEAHNEAEMEQVLACGAKICGINNRNLKNFVVDLKTTERLAAMVPKDCILVSESGMQTHEDLAAVRKYGADAVLIGETLMRSGDVAVAMQQLRENL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5V773</accession>
    <name>R5V773_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Heterodisulfide reductase subunit C-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_01678</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000175">
        <property type="protein sequence ID" value="CCZ83949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01678"/>
    <dbReference type="EnsemblBacteria" id="CCZ83949">
        <property type="protein sequence ID" value="CCZ83949"/>
        <property type="gene ID" value="BN566_01678"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13183">
        <property type="entry name" value="Fer4_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="10"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ83949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="126" mass="14395" checksum="CE5DECB71B1B9232" modified="2013-07-24" version="1">MADWKEQVLRTSGVNPLKCMRCGKCSATCPSYDAMEYHPHQFVYMVEKGNIEPLMQSESIYKCLSCFACLERCPRNVEPAKLVEAVRLAVIRQQGQNHLKPEQIPAMLDEDLPQQAIVSAFRKYSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7Y2</accession>
    <name>R5V7Y2_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Magnesium transporter MgtE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN566_01959</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Acts as a magnesium transporter.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the SLC41A transporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01959"/>
    <dbReference type="EnsemblBacteria" id="CCZ84234">
        <property type="protein sequence ID" value="CCZ84234"/>
        <property type="gene ID" value="BN566_01959"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.60.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006668">
        <property type="entry name" value="Mg_transptr_MgtE_intracell_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038076">
        <property type="entry name" value="MgtE_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006669">
        <property type="entry name" value="MgtE_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006667">
        <property type="entry name" value="SLC41_membr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036739">
        <property type="entry name" value="SLC41_membr_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43773">
        <property type="entry name" value="PTHR43773"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01769">
        <property type="entry name" value="MgtE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03448">
        <property type="entry name" value="MgtE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00924">
        <property type="entry name" value="MgtE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161093">
        <property type="entry name" value="SSF161093"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00400">
        <property type="entry name" value="mgtE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0129">CBS domain</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="291"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="331"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="369"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="395"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="448"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="8">
        <location>
            <begin position="145"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="8">
        <location>
            <begin position="209"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ84234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="469" mass="52196" checksum="65D220C5A40B367C" modified="2013-07-24" version="1">MEKMLATMTTKPNYESEIIKIVKGNGSPKVILNQLENYHGSDIADVIEQLNEQERKKIFRICPAEMLAEAFSHLEETQAGAYLNEIPLKKVTEILAELETDAAVEILHTIGKEKRELIIDALDEDIRKEIQLIASFDEDEIGSKMTTNCIILRENLTVKDAMKELVRQAEENDNISTLFVVDDTDCFYGAIALKDLITARSHVPLEHLIATSFPYVYANETIDACMEQLKDYSEDLIPVLDNQNKLLGVITAQSLIDVVDEEMGEDYAKLAGLTAEEDLNEPLKQSMKKRLPWLFALLLLGLLVSVVVGAFEKVVAQLTLIMAFQSLILDMSGNVGTQSLAVTIRVLMDENLTFQKKLKLIGKEMRVGFFNGLLLGVLSFVMVGLYIMLFKHKTILFSFAISGCIGVSLILAMLISSAVGTLIPLFFKKIRVDPAVASGPLITTVNDLIAVVTYYGMSWILLLNILKLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>R5V825</accession>
    <name>R5V825_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator MarR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00023</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000208">
        <property type="protein sequence ID" value="CCZ84284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00023"/>
    <dbReference type="EnsemblBacteria" id="CCZ84284">
        <property type="protein sequence ID" value="CCZ84284"/>
        <property type="gene ID" value="BN566_00023"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12802">
        <property type="entry name" value="MarR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00598">
        <property type="entry name" value="HTHMARR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="10"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="160" mass="18681" checksum="055A7434E59D88F6" modified="2013-07-24" version="1">MKKQVQPESPFRLFPPLHQLCTLMTRYCNRSRGIREQEQLTGVHGWLIHFLYDQTEPVYQRDIEKRLCIRRSTVTATLNRMEKNGLIVRESVPEDARLKRIVLTEKAISLHNDVEKECQFFESVLTAGISMEDLEQFQKTLQVMCKNLTDYESKGGNSTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5V8J4</accession>
    <name>R5V8J4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Methylenetetrahydrofolate reductase</fullName>
            <ecNumber evidence="10">1.5.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN566_00341</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2 10">
            <text>(6S)-5-methyl-5,6,7,8-tetrahydrofolate + NAD(+) = (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:19821"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:18608"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.5.1.20"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>(6S)-5-methyl-5,6,7,8-tetrahydrofolate + NADP(+) = (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:19817"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:18608"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.5.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3 10">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 10">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 10">Belongs to the methylenetetrahydrofolate reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.20" evidence="10"/>
    <dbReference type="EMBL" id="CBAR010000225">
        <property type="protein sequence ID" value="CCZ84434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00341"/>
    <dbReference type="EnsemblBacteria" id="CCZ84434">
        <property type="protein sequence ID" value="CCZ84434"/>
        <property type="gene ID" value="BN566_00341"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106312">
        <property type="term" value="F:methylenetetrahydrofolate reductase NADH activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106313">
        <property type="term" value="F:methylenetetrahydrofolate reductase NADPH activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00537">
        <property type="entry name" value="MTHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029041">
        <property type="entry name" value="FAD-linked_oxidoreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003171">
        <property type="entry name" value="Mehydrof_redctse-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004620">
        <property type="entry name" value="MTHF_reductase_bac"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02219">
        <property type="entry name" value="MTHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51730">
        <property type="entry name" value="SSF51730"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00676">
        <property type="entry name" value="fadh2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0274">FAD</keyword>
    <keyword evidence="6 10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="8 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ84434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="291" mass="32178" checksum="B11B68B808C01015" modified="2013-07-24" version="1">MKIAPLFSEKTVFSFEVFPPKKTSPIETVYQTLEELQDLHPDFISVTLGAGGSGNGENTIAIARRLQEQYGITSMSHLPCIHCTKEKIDALLETFLESGIENILALRGDRVPELEPEHDFAHASDLITYIREKHPNFDIAGACYPECHTEATSLEEDIANLKKKVDAGATHLISQLFFDNEAFFHFQAEARKAGITVPIEAGIMPVTNSKQITRMVSMCGASLPRKFTRIIQKYEDNPTALQDAGIAYAIAQIIELLAAGVDGIHLYTMNNPYVARKISEAISGVLRATQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>R5V8W1</accession>
    <name>R5V8W1_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">50S ribosomal protein L29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpmC</name>
        <name evidence="7" type="ORF">BN566_00065</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the universal ribosomal protein uL29 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000241">
        <property type="protein sequence ID" value="CCZ84519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00065"/>
    <dbReference type="EnsemblBacteria" id="CCZ84519">
        <property type="protein sequence ID" value="CCZ84519"/>
        <property type="gene ID" value="BN566_00065"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00374">
        <property type="entry name" value="Ribosomal_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001854">
        <property type="entry name" value="Ribosomal_L29/L35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036049">
        <property type="entry name" value="Ribosomal_L29/L35_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018254">
        <property type="entry name" value="Ribosomal_L29_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00831">
        <property type="entry name" value="Ribosomal_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46561">
        <property type="entry name" value="SSF46561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00012">
        <property type="entry name" value="L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00579">
        <property type="entry name" value="RIBOSOMAL_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="11"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ84519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="67" mass="7604" checksum="0089C53F62FBE040" modified="2013-07-24" version="1">MKASEVRAMPVDELNEKLVDLKEELFNLRFQLAANQLENTARIKAVKKDIARVKTALRAAELQSAQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5V926</accession>
    <name>R5V926_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00457</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000255">
        <property type="protein sequence ID" value="CCZ84604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84604">
        <property type="protein sequence ID" value="CCZ84604"/>
        <property type="gene ID" value="BN566_00457"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="88" mass="8622" checksum="91BA834F535E1248" modified="2013-07-24" version="1">MEVMSEYGCKSISVSKATSVNVNAASPDGLVCGSVVVVCAEVVVSAGVESVAVVVPVVVVVVSVVVVVVAVVVAVVAGVVVGVTVPWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5V939</accession>
    <name>R5V939_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Cna protein B-type domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN566_00472</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000257">
        <property type="protein sequence ID" value="CCZ84619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00472"/>
    <dbReference type="EnsemblBacteria" id="CCZ84619">
        <property type="protein sequence ID" value="CCZ84619"/>
        <property type="gene ID" value="BN566_00472"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026466">
        <property type="entry name" value="Fim_isopep_form_D2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032334">
        <property type="entry name" value="GramPos_pilinBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019931">
        <property type="entry name" value="LPXTG_anchor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041033">
        <property type="entry name" value="Prealbumin-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00746">
        <property type="entry name" value="Gram_pos_anchor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16569">
        <property type="entry name" value="GramPos_pilinBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17802">
        <property type="entry name" value="SpaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04226">
        <property type="entry name" value="RrgB_K2N_iso_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="1" id="KW-0134">Cell wall</keyword>
    <keyword evidence="3" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0572">Peptidoglycan-anchor</keyword>
    <keyword evidence="3" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0964">Secreted</keyword>
    <keyword evidence="2 7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004406829" evidence="7">
        <location>
            <begin position="33"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="525"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="domain" description="GramPos_pilinBB" evidence="9">
        <location>
            <begin position="230"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="SpaA" evidence="10">
        <location>
            <begin position="398"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="GRAM_POS_ANCHORING" evidence="8">
        <location>
            <begin position="510"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="206"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="206"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ84619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="553" mass="57988" checksum="029008DD8E1E388E" modified="2013-07-24" version="1">MKNTKRARRAAAFAAAVVMAACAAIPMGSSFSASAVATENTISFTGQEGTGTHTYQAYMIFSGTAVANGFGGAAELQGIQWANSTGAAEFLTALKDDATIGTDFADCTTAAAVADVLKGYSVNSAKAKAFADLAIANKEKLKAVGDAGSTITAAEDGYYVIEETGVTAGTSTMTAHLLGVYDASAGAEITVKSSLPTFEKKIMDSNDSGTTVQDGSTNGAWQDSADHDFGDKVPFQLTGTLPGDYASYKQYKMVFHDNLQDGVYGSPENVKVYYKHGDTTKEISSDKYTISNPNTADDGFADTHGDKTCNLEINIANLKTATGDVTLQAGDKIIVEYQAELLTAANMGEAGNWNSGYLEYSNNPYHTGEGDTTSKTPEDMVVDFTYQLAVNKVDNALKPLKGAGFTLYKYNGTVYEAVGTEITGVTTFEFKGVDAGQYKLVETTTPKGFNTVDELEFIVKAEHDETSDTPTLTSLTVVDTDGNSLTATNTEAGKFVVTKKDGLAATQIINNSGSTLPSTGGIGTTMFYVGGGVLVAGAGVILITKKRAKKDAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5V964</accession>
    <name>R5V964_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00502</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000265">
        <property type="protein sequence ID" value="CCZ84649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84649">
        <property type="protein sequence ID" value="CCZ84649"/>
        <property type="gene ID" value="BN566_00502"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004406859" evidence="2">
        <location>
            <begin position="27"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="246"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="274" mass="28175" checksum="964BE6477A2997BD" modified="2013-07-24" version="1">MKRIIKGVLAAALVATALTAGTISFAANTEVSAIEGVTATLTGSLDDSNFAYYGVGKTNKNIESTTANISSDGEYVISWTVSGNASTVDYLYLDLNFPSSINTQPIKDTFPDLNIVVTEVKTGSTIVDYKMGTNAVNYDYLNGGLDSVRVTLCDQSTANNGYLSKQTAIGQTLSVTFQVSGLGDDTTTTPVTTTTMPVQTTPIQTTMATTLPVTQPATPVVTTTAVVAAGSSSTGGIPTPTQTADLSVGAIVIGGVAAVSLAGAAFTVTRRKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>R5VGY8</accession>
    <name>R5VGY8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">LysR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00641</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000012">
        <property type="protein sequence ID" value="CCZ82836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00641"/>
    <dbReference type="EnsemblBacteria" id="CCZ82836">
        <property type="protein sequence ID" value="CCZ82836"/>
        <property type="gene ID" value="BN566_00641"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ82836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="308" mass="36006" checksum="F46EE713F12A0701" modified="2013-07-24" version="1">MLDYRIYTFISLCETRSYTKTAEQLHISQPSVSQHIKALEQHYGCDLFYFEGRNLKLTKEGRYLYQKEVGIMTNEKEIESYIEQMANGKHLRIGMTPSVSESFVPKYLAAYIRKNAEIHFQFLVDDMQQLRGNLANGTLDVVFSEQFFLNGSIFEQHIYHQEQLTLVSDPATAARLYGRSVSAILKETLLLPPEGSGLREIIMHWLNSRSMALTDFERVIVVNSITMIREMLAQQIGVSILFESNVREDVQNRRLQRLQMDDFFLWGNFQFVYLKESLSKDLYTDFFQDFQNMVTEHAHLPTVPYQFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>R5VH50</accession>
    <name>R5VH50_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RNA polymerase sigma factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN566_00688</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the sigma-70 factor family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000022">
        <property type="protein sequence ID" value="CCZ82886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00688"/>
    <dbReference type="EnsemblBacteria" id="CCZ82886">
        <property type="protein sequence ID" value="CCZ82886"/>
        <property type="gene ID" value="BN566_00688"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030435">
        <property type="term" value="P:sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000943">
        <property type="entry name" value="RNA_pol_sigma70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007630">
        <property type="entry name" value="RNA_pol_sigma70_r4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04545">
        <property type="entry name" value="Sigma70_r4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000770">
        <property type="entry name" value="RNA_pol_sigma-SigE/K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00046">
        <property type="entry name" value="SIGMA70FCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00715">
        <property type="entry name" value="SIGMA70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00716">
        <property type="entry name" value="SIGMA70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="3" id="KW-0749">Sporulation</keyword>
    <keyword evidence="7 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="9">
        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ82886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="231" mass="26794" checksum="923822587713A1BA" modified="2013-07-24" version="1">MFLSALFQQLLCFFLHVETSGAFRKPLSQKEELRCFQQMRAGDALAREKLILHNMRLVAHIIKKYYTNQTEQEDLLSIGTVGLMKAVSTFDYEKGARFATYASRCIENEILMHFRTCRKQGDAISMAEPIDTDKDGNQLTLLDIIEDGCDIQEQVASQMQIQQLYQFLQQKLTPREVEILVHRYGLYGQIPMTQREVAKKMGISRSYVSRLEKRAIAMLRKQYDQTPFSSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5VH84</accession>
    <name>R5VH84_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cellobiose phosphorylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00102</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000024">
        <property type="protein sequence ID" value="CCZ82911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00102"/>
    <dbReference type="EnsemblBacteria" id="CCZ82911">
        <property type="protein sequence ID" value="CCZ82911"/>
        <property type="gene ID" value="BN566_00102"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11754">
        <property type="entry name" value="GH94N_CBP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009342">
        <property type="entry name" value="Carb-bd_put_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033432">
        <property type="entry name" value="GH36_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037825">
        <property type="entry name" value="GH94N_CBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037018">
        <property type="entry name" value="Glyco_hydro_65_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010383">
        <property type="entry name" value="Glyco_hydrolase_94"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17167">
        <property type="entry name" value="Glyco_hydro_36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06165">
        <property type="entry name" value="Glyco_transf_36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01068">
        <property type="entry name" value="CBM_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="SSF48208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="SSF74650"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CBM_X" evidence="2">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ82911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="817" mass="92547" checksum="9E42B833CB6C5F61" modified="2013-07-24" version="1">MKFGHFDDVNKEYVIQNPKTPYPWINYLGTQAFFSLISNTAGGYHFYKDARLRRITRYRYNNVPVDMGGRYFYIKDNDTIWSPGWAPVKTELDSYECRHGMGYTVITGKKNGLSAEVTFFVPQDYNGEIQSVVLKNESNEAKTFKLFSFVEWCLWNAQDDSTNFQRNFNTGEVEIVDSTIFHKTEYKERRNHFAFYTVNDTIDGYDTDRESFLGLYNGFENPQVVAAGKASNSVADGWSPIASHYKEITLQPGESKQLVFILGYVENPVEEKFNADGSMNKSRAYAMMEQYNTPEKVAAGLAALKEMWNNLLSKYTVKTPDDKMNRMVNIWNQYQCMVTFNMSRSASYFESGIGRGMGFRDSNQDLLGFVHQIPDRARERLIDLASTQFEDGGCYHQYQPLTKKGNNEIGGDFSDDPLWMILSVAAYIKESGDYSILDAKVPYDNDENKAKTMMDHLEKSFYHVFENVGPHGLPLAMRADWNDCINLSCFSSEPGESFQTSTSPKYGEDKYSKIAESVMVAALFTYAGPEYVAMCRLKGDDAAADKAQAAVDKMKDNIMKYGWDGEWFLRAYDDFGKKMGSHECEEGKIFIEPQGFAVMGGCGKETGADLKALESVDKWLNSEHGLSLNQPAFTKYYIEYGEISTYPAGYKENAGIFCHNNAWIICAEAFVGHGDKAFEYYSKIAPAYREEKYSDLHRTEPYVYAQMIAGKDAKRHGEAKNSWLTGTAAWNFVAVSQYILGIIPDWNGLKVDPSIPHEWDGFTVSRQFRGNTYEITVKNPKHICKGVQSVTVDGKQIEGNVLPVFDGGVHTVEVVMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5VHT7</accession>
    <name>R5VHT7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Conserved protein/domain typically associated with flavoprotein oxygenases DIM6/NTAB family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00859</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000051">
        <property type="protein sequence ID" value="CCZ83071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83071">
        <property type="protein sequence ID" value="CCZ83071"/>
        <property type="gene ID" value="BN566_00859"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016646">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-NH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002563">
        <property type="entry name" value="Flavin_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01613">
        <property type="entry name" value="Flavin_Reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00903">
        <property type="entry name" value="Flavin_Reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavin_Reduct" evidence="2">
        <location>
            <begin position="12"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="183"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="205" mass="22826" checksum="BD6D1861A7BD4047" modified="2013-07-24" version="1">MAKQHWKGSVLLAPVPPVLVSCGTMEHPNILTIAWTGVLNTHPPMVSISVRPTRYSYPILMEQREFVINLPTSAMCRETDFCGCRSGKDIDKFQACHFTPEPAQTVSAPLIAECPVSLECKIQSTQLLGTHELMLAEITAVNVEERYVDSKGKLNLQQMGLLAYAHGEYFSLGRKLGDFGYSVRKKKKTPKKPPQNKKQLPSQKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5VHU7</accession>
    <name>R5VHU7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Fructose-2 6-bisphosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00879</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000053">
        <property type="protein sequence ID" value="CCZ83091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00879"/>
    <dbReference type="EnsemblBacteria" id="CCZ83091">
        <property type="protein sequence ID" value="CCZ83091"/>
        <property type="gene ID" value="BN566_00879"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="SSF53254"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="206" mass="23087" checksum="290BA6EB77A9CDD6" modified="2013-07-24" version="1">MKGYRISLIRHGRTEANDKGIYIGRTDYPLSQKGISELYNKLDEFEYPHVGKVYSSPLQRCTETAEILFPDSSVQIVENLIEMDFGDFEGKSADELVKKPEFLEWLKGGADSRPPKGESLQEVQLRLFKALRTIILDMMQNDILHTAVVTHAGIITNLIAGFGLPKIDPKELRPAPGEGYDILVTASLWQRSGAFEILGMTPYANN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>R5VHW9</accession>
    <name>R5VHW9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Dihydroorotase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_00913</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the reversible cyclization of carbamoyl aspartate to dihydroorotate.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the metallo-dependent hydrolases superfamily. DHOase family. Class I DHOase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000061">
        <property type="protein sequence ID" value="CCZ83126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00913"/>
    <dbReference type="EnsemblBacteria" id="CCZ83126">
        <property type="protein sequence ID" value="CCZ83126"/>
        <property type="gene ID" value="BN566_00913"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004151">
        <property type="term" value="F:dihydroorotase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006221">
        <property type="term" value="P:pyrimidine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01317">
        <property type="entry name" value="DHOase_IIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004722">
        <property type="entry name" value="DHOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002195">
        <property type="entry name" value="Dihydroorotase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00483">
        <property type="entry name" value="DIHYDROOROTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="6">
        <location>
            <begin position="61"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ83126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="332" mass="36547" checksum="B27E4665717AE38A" modified="2013-07-24" version="1">MPNTKPTIDTPETVQYILDKAAPTGVKVHPIGCITNGMKGESLCDYPALMQAGICAISDDGRPVENAEQMRLALEQARDLHLPVISHCEDLSIIHGGIVNKGAVSEQLQVQGMDRASEDYITAREIILAGSVHGAIHIAHVSTKGSVEIVRLAKREGIAVTCETCPHYFLLTEEKVLSKDADYRMSPPLRTEEDRKAVLEGVLDGTIDCIVTDHAPHTPEEKSDFYHAPNGVIGMETSLAATLTLYHQNLLTLPDIVRLMADRPREILHLPKQTLEAGAPANLTLVDLNKEWIVDPNKLHSKARNAVFKQMQFRGKAVLTMTDGVIRYRDKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>R5VHX2</accession>
    <name>R5VHX2_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Dihydroorotate dehydrogenase</fullName>
            <shortName evidence="9">DHOD</shortName>
            <shortName evidence="9">DHODase</shortName>
            <shortName evidence="9">DHOdehase</shortName>
            <ecNumber evidence="9">1.3.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">pyrD</name>
        <name evidence="11" type="ORF">BN566_00918</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the conversion of dihydroorotate to orotate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(S)-dihydroorotate + A = AH2 + orotate</text>
            <dbReference type="Rhea" id="RHEA:18073"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
            <dbReference type="ChEBI" id="CHEBI:30864"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="9">Binds 1 FMN per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Pyrimidine metabolism; UMP biosynthesis via de novo pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the dihydroorotate dehydrogenase family. Type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.-.-" evidence="9"/>
    <dbReference type="EMBL" id="CBAR010000062">
        <property type="protein sequence ID" value="CCZ83131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00918"/>
    <dbReference type="EnsemblBacteria" id="CCZ83131">
        <property type="protein sequence ID" value="CCZ83131"/>
        <property type="gene ID" value="BN566_00918"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00070"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004152">
        <property type="term" value="F:dihydroorotate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04740">
        <property type="entry name" value="DHOD_1B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00224">
        <property type="entry name" value="DHO_dh_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033888">
        <property type="entry name" value="DHOD_1B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005720">
        <property type="entry name" value="Dihydroorotate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024920">
        <property type="entry name" value="Dihydroorotate_DH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012135">
        <property type="entry name" value="Dihydroorotate_DH_1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001295">
        <property type="entry name" value="Dihydroorotate_DH_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01180">
        <property type="entry name" value="DHO_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000164">
        <property type="entry name" value="DHO_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01037">
        <property type="entry name" value="pyrD_sub1_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00911">
        <property type="entry name" value="DHODEHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00912">
        <property type="entry name" value="DHODEHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6 9" id="KW-0288">FMN</keyword>
    <keyword evidence="8 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7 9" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHO_dh" evidence="10">
        <location>
            <begin position="4"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="9">
        <location>
            <begin position="69"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="9">
        <location>
            <begin position="191"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="9">
        <location>
            <position position="129"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="45"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="242"/>
            <end position="243"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ83131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="303" mass="31911" checksum="E905B02139CCBDAA" modified="2013-07-24" version="1">MTDLSVQVAGVPFKNPIIPASGVFGYGHEYEELFPLSKLGGIATKGTTITPRPGNPSPRIAETPSGMLNSVGLQNPGIDAFIERELPYLLTKDTVILANIAGSTIEECVTIAERLDQTDVHMIELNISCPNVKKGGAAFGVHCDSAERITAAVRQATKKPLVVKLSPNVTDITEIAKAVEAAGADAVSLINTLLGMRIDIRSKRPILRNNVGGMSGPAVFPIAVRMVWQVSNAVSIPVIGMGGVDSGAAAMEMMLAGASAVQVGAAIFTDPYAPVRIVEELQALLEEQHIADVKELIGAVKPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5VIE1</accession>
    <name>R5VIE1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01112</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000089">
        <property type="protein sequence ID" value="CCZ83331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01112"/>
    <dbReference type="EnsemblBacteria" id="CCZ83331">
        <property type="protein sequence ID" value="CCZ83331"/>
        <property type="gene ID" value="BN566_01112"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026350">
        <property type="entry name" value="GxxExxY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13366">
        <property type="entry name" value="PDDEXK_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="516" mass="60596" checksum="B65EE1AD1201E0CB" modified="2013-07-24" version="1">MERYFNITGCCNPQEHYMVNLDSRLAQIKKMVDRGQYFCINRGRQYGKTTLIMALESYLEKEYIVVSMDFQGLSTPEFSTEGMFVKAFARELWLDRRTQHFMNHDIQTAMKEIRSSSAYTMADLFVCLSEWCMEAEKKIVLMIDEVDQASNNQVFLDFLAQLRFYYLKRNKRPTFQSVILAGVHDIRNLRQKIRPDAEHKHNSPWNIASNFDIDMSFSVSDIAGMLTEYEQDHHTGMDIVKLSQLIYDYTSGYPVLVSSICKYMDENQSWNDLSFENAVKYLVKEKNPLVESLINKLEDDKNLRNLLYNVLFLGQKISYNIDNTVIENAAMYGFISNTNDCVSVANRIFETRIYNWFISQEATDNRMYSEGVNDKNQFIQGNQLNMEKILEKFILHFNDIYGSQPDKFKEEDGRKLFLLYLRPIINGTGNYYIEAQTRDQKRTDVIVDYLGKQYIIELKIWRGEEYNTEGEKQIAEYLNYYHLEKGYLLSFNFNKNKKSGMKTIQMNGKTVIEAVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5VIK9</accession>
    <name>R5VIK9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">C_GCAxxG_C_C family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01162</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000094">
        <property type="protein sequence ID" value="CCZ83386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01162"/>
    <dbReference type="EnsemblBacteria" id="CCZ83386">
        <property type="protein sequence ID" value="CCZ83386"/>
        <property type="gene ID" value="BN566_01162"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010181">
        <property type="entry name" value="CGCAxxGCC_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09719">
        <property type="entry name" value="C_GCAxxG_C_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01909">
        <property type="entry name" value="C_GCAxxG_C_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="153" mass="16429" checksum="C27B9DF258EC0D10" modified="2013-07-24" version="1">MSIYTEKAVALFREGYNCAQAVTGAFAEPFGMSQEDALRFSEGMGAGMGRLRLTCGAVSAMALLAGLKLSNGKPNDLTTRKEVYAVVQEMTKAFQEKNGSIICAELLGAARPKHETAQPEARTAAYYQKRPCVGCVEDCAEIVEQYLCGAGEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5VIS4</accession>
    <name>R5VIS4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Radical SAM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01205</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000103">
        <property type="protein sequence ID" value="CCZ83431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83431">
        <property type="protein sequence ID" value="CCZ83431"/>
        <property type="gene ID" value="BN566_01205"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040085">
        <property type="entry name" value="MJ0674-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43075">
        <property type="entry name" value="PTHR43075"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="241" mass="27139" checksum="D59CE9E876A82C5B" modified="2013-07-24" version="1">MRCCFCQNRQISQEWFGKAISVSQLSEILLRLQEQGAETLDLVTGSHYAPWIAESLQQVKPQLHIPVVWNSSGYDSPEILSLLNGLVDVYLPDWKYVDNATAEQYSGISDYCTVTEQAISEMVRQVGTPVLNHGILQHGVLIRHLILPGQRHASMALLRKIAATFPDGQVWVSLMGQYTPPETPLPDRHLNRRLTTMEYQSVLKVAQELELEGFSQDLTSARKTYIPSFSLEGLDEPSQKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>R5VIZ0</accession>
    <name>R5VIZ0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_01245</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000114">
        <property type="protein sequence ID" value="CCZ83476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01245"/>
    <dbReference type="EnsemblBacteria" id="CCZ83476">
        <property type="protein sequence ID" value="CCZ83476"/>
        <property type="gene ID" value="BN566_01245"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="44"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="130"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="335" mass="38595" checksum="825C72F6C7FB0666" modified="2013-07-24" version="1">MQHKKRARILLQFFWEQRMVGLFLILAISIFALVFSMYDIKLEAILYASMLCLTAGLLFEGVHLISYLRRHTEQQKRLQGLPVFYSELPPPRTLAEQDLQAMVQKLGEQYTAVTTDWQRQQKESLDYYSAWVHQIKTPISSMKMILQQEDTEENHLLSAELFRIEQYTEMALQYLRLDSKTNDFVFQQYDLDSIIRQAIRKYAPQFILRKIRLIYEPVSMTILTDEKWMLFLLEQLLSNAIKYTPHGSVTISVTPEKVLQVADTGIGIAPEDLPRIFEKGFTGYNGRADKKSTGLGLYLCQQAAKKISVSLSVTSEVGKGSVFSVHLDIPPLQVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5VIZ7</accession>
    <name>R5VIZ7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Inorganic pyrophosphatase</fullName>
            <ecNumber evidence="5">3.6.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Pyrophosphate phospho-hydrolase</fullName>
            <shortName evidence="5">PPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ppa</name>
        <name evidence="6" type="ORF">BN566_01250</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the hydrolysis of inorganic pyrophosphate (PPi) forming two phosphate ions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>diphosphate + H2O = H(+) + 2 phosphate</text>
            <dbReference type="Rhea" id="RHEA:24576"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="EC" id="3.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.1" evidence="5"/>
    <dbReference type="EMBL" id="CBAR010000116">
        <property type="protein sequence ID" value="CCZ83481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01250"/>
    <dbReference type="EnsemblBacteria" id="CCZ83481">
        <property type="protein sequence ID" value="CCZ83481"/>
        <property type="gene ID" value="BN566_01250"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004427">
        <property type="term" value="F:inorganic diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006796">
        <property type="term" value="P:phosphate-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00412">
        <property type="entry name" value="pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00209">
        <property type="entry name" value="Inorganic_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008162">
        <property type="entry name" value="Pyrophosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036649">
        <property type="entry name" value="Pyrophosphatase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10286">
        <property type="entry name" value="PTHR10286"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00719">
        <property type="entry name" value="Pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50324">
        <property type="entry name" value="SSF50324"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="5">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ83481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="182" mass="20735" checksum="CEC7714C71EBD9C0" modified="2013-07-24" version="1">MNIWHDIEPERIYPTDFTAVVEISKGSNMKYELDKKTGMLSLDRVLFTATYYPMNYGFIPRTYGNDNDPLDVLILCSAPIQPMTLVRCYPIGVMTMEDSGMGDEKIIAIPYSDPTYMSYTSVDELPKHIFQELKHFFSVYKQLEGKKTEVNDIGGPTEAIAIIQEAMDSYQKKFGSAQNQEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJ68</accession>
    <name>R5VJ68_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">50S ribosomal protein L28</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpmB</name>
        <name evidence="5" type="ORF">BN566_01305</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the bacterial ribosomal protein bL28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000121">
        <property type="protein sequence ID" value="CCZ83536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01305"/>
    <dbReference type="EnsemblBacteria" id="CCZ83536">
        <property type="protein sequence ID" value="CCZ83536"/>
        <property type="gene ID" value="BN566_01305"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.170.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00373">
        <property type="entry name" value="Ribosomal_L28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034704">
        <property type="entry name" value="L28p-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026569">
        <property type="entry name" value="Ribo_L28/L24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037147">
        <property type="entry name" value="Ribo_L28/L24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001383">
        <property type="entry name" value="Ribosomal_L28"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00830">
        <property type="entry name" value="Ribosomal_L28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143800">
        <property type="entry name" value="SSF143800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00009">
        <property type="entry name" value="L28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="61" mass="6903" checksum="BEA9DB85867D8259" modified="2013-07-24" version="1">MAKCEFCGKGVTFGIKVSHSHRRSNRTWKPNVKRVKAVVKGTPCHVYVCSRCLRSGKVERV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJE3</accession>
    <name>R5VJE3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH cro/C1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01382</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000147">
        <property type="protein sequence ID" value="CCZ83646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83646">
        <property type="protein sequence ID" value="CCZ83646"/>
        <property type="gene ID" value="BN566_01382"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="10"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="132" mass="14633" checksum="DD92C23C0D4E0D1F" modified="2013-07-24" version="1">MNNAQLAGIIKELCKSRGTSVSKMLSECGIRKSLIYDLEKRDWTPSISVAEQIANYFDCSVDYLLGRTDNPTVSGNNISNFDTTINGTQANIIQGAETDLDEIKVALSKLTSSNRHRAIADMLDLLEKYIEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJF6</accession>
    <name>R5VJF6_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">Single-stranded DNA-binding protein</fullName>
            <shortName evidence="2">SSB</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_01397</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000148">
        <property type="protein sequence ID" value="CCZ83661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01397"/>
    <dbReference type="EnsemblBacteria" id="CCZ83661">
        <property type="protein sequence ID" value="CCZ83661"/>
        <property type="gene ID" value="BN566_01397"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04496">
        <property type="entry name" value="SSB_OBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00984">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000424">
        <property type="entry name" value="Primosome_PriB/ssb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011344">
        <property type="entry name" value="ssDNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10302">
        <property type="entry name" value="PTHR10302"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00436">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00621">
        <property type="entry name" value="ssb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50935">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="105"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ83661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="189" mass="21176" checksum="D6C62AD23667DDAB" modified="2013-07-24" version="1">MINKVILMGRLCADPELKTTQSGIAVCRFRIAVNRQYSKSSEKKADFINIVSWRQQAEFVSRYFRKGSMIIVEGKLQNADYTDNNGVKHYAMDVQADNVTFGESKTAQNAAQSDYNSQPQNYQPTPQTAYSEPQTPSYSNPMQDVVNQSQNVVHTYEADVKNAGKSTPEIDLDDLSDFQTILGDGDVPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5VK54</accession>
    <name>R5VK54_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rqc2 homolog RqcH</fullName>
            <shortName evidence="1">RqcH</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rqcH</name>
        <name evidence="3" type="ORF">BN566_01645</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the ribosome quality control system (RQC). Recruits Ala-charged tRNA and directs the elongation of stalled nascent chains on 50S ribosomal subunits, leading to non-templated C-terminal Ala extensions (Ala tail). The Ala tail promotes nascent chain degradation. May add between 1 and at least 8 Ala residues. Binds to stalled 50S ribosomal subunits.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Associates with stalled 50S ribosomal subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NEMF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000172">
        <property type="protein sequence ID" value="CCZ83916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01645"/>
    <dbReference type="EnsemblBacteria" id="CCZ83916">
        <property type="protein sequence ID" value="CCZ83916"/>
        <property type="gene ID" value="BN566_01645"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00844_B">
        <property type="entry name" value="RqcH_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008532">
        <property type="entry name" value="NFACT_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043682">
        <property type="entry name" value="RqcH_bacterial"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05670">
        <property type="entry name" value="NFACT-R_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="NFACT-R_1" evidence="2">
        <location>
            <begin position="466"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="582" mass="65649" checksum="70AC83D708CCBECB" modified="2013-07-24" version="1">MALDGAFLYAVRQELSGLIGSRIEKIHQPAREEILISLRNMEGSHKILISASADRARVHLTQQSIDNPPAPPMFCMLLRKRLGNGKLLAIRQDGLERVLYFDFSCVNTLGDVVQLTLACEIMGKYSNLILIDETGKVVDSIKRVDAEMSRKRLVLPGIAYALPPAEPRLNFLTDSMKTIQAAITAQTGALPKILLKTLEGVSPVLVREWAFYAGNGEECRAESLTAVQLKRLLEIMQQTKERLLQQDCVFTVAATKEGQLKDFSFLPLHQYGTLLITKTVPSACAVLDYFYAERDRYARVRQRANDLFHLLLHATERIQRRIAAQTEERTQCAEKDTFRRKADLLSANLYRLKKGDTVAELEDFYEPDCPMVSIPLDVRLTPPQNAQRYYQQYKKACTAETVLTEQIAKGKAELAYLESVLDALTRAETEADMEQLRQELIEQGYLRKTSRNRRPVKIQQPLSVTATDGTQILIGRNNLQNDRLTLKTAAKTDVWLHTQNIPGSHVIICTHGETPSEQTILEAAQLAAWYSKAQQSAQVPVDYCLVKYVKKPVGAKPGMVIFTNQRTLYVTPRQTLEEEETI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>R5VKH8</accession>
    <name>R5VKH8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DNA polymerase III PolC-type</fullName>
            <shortName evidence="11">PolIII</shortName>
            <ecNumber evidence="11">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">polC</name>
        <name evidence="15" type="ORF">BN566_01725</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Required for replicative DNA synthesis. This DNA polymerase also exhibits 3' to 5' exonuclease activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the DNA polymerase type-C family. PolC subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="11"/>
    <dbReference type="EMBL" id="CBAR010000178">
        <property type="protein sequence ID" value="CCZ83996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01725"/>
    <dbReference type="EnsemblBacteria" id="CCZ83996">
        <property type="protein sequence ID" value="CCZ83996"/>
        <property type="gene ID" value="BN566_01725"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00356">
        <property type="entry name" value="DNApol_PolC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011708">
        <property type="entry name" value="DNA_pol3_alpha_NTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040982">
        <property type="entry name" value="DNA_pol3_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024754">
        <property type="entry name" value="DNA_PolC-like_N_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029460">
        <property type="entry name" value="DNAPol_HHH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006054">
        <property type="entry name" value="DnaQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Exonuclease_RNaseT/DNA_pol3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004013">
        <property type="entry name" value="PHP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003141">
        <property type="entry name" value="Pol/His_phosphatase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006308">
        <property type="entry name" value="PolC_gram_pos"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044923">
        <property type="entry name" value="PolC_middle_finger_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11490">
        <property type="entry name" value="DNA_pol3_a_NII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07733">
        <property type="entry name" value="DNA_pol3_alpha"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17657">
        <property type="entry name" value="DNA_pol3_finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14579">
        <property type="entry name" value="HHH_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02811">
        <property type="entry name" value="PHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00481">
        <property type="entry name" value="POLIIIAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00573">
        <property type="entry name" value="dnaq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01405">
        <property type="entry name" value="polC_Gram_pos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 11" id="KW-0235">DNA replication</keyword>
    <keyword evidence="9 11" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="8 11" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="7 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 11" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4 11" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="POLIIIAc" evidence="14">
        <location>
            <begin position="343"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="Exonuclease" evidence="13">
        <location>
            <begin position="432"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="174"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="174"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CCZ83996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="1450" mass="163248" checksum="45B88364B0D74AE9" modified="2013-07-24" version="1">MKGVHPMETLRFSDFLDSYIQTMPDSMQDGMLYRLTYAENLTRLTFYVTFPQLVPANDLFSLEKTLQQALEIETVRIAPNYAATLCQTAYFPELLQLLKREQPVVNGFFDHAQIQQDGQNWTITLKNGGYEIVQRYHVAEQLQNLLQRLFSVSIKVEILGEAAVSTESYQQMQETAEKTVSKHTEQLQATAPTKSSDKAEPAKSKPVALSVELENVVPGSATLIKGRTIRDNPISITEALGREGQRSVILADVFAMEVREVRGERTVATYQVTDYENSVLVKLFDTTENLKKIPLSEIKKGSTLLLLGRIDYDNFAREMVLKPDSIMMVQRKKKEDHAEKKRVELHCHTAMSQMDAITEAGKLVNRAHEWGHPAIAITDHGIAQAFPDAMNAWNATKDPNFKIIYGCEAYVVNDLQKKTIIDESDPRSLQDEIIIFDVETTGLSYTKDRLTEIGAVKLRNLQIVDTFSIMVNPGKPIPPKIVELTGITDQMVANAPGEREAIQQFMEFCGSEKPVLAAHNARFDTSFIRNACERQNISFSFVTLDTLILSQVMLPNIAKHKLDSVAKALKLGKFDHHRACNDAQMLAKIYVKLVSQLIQEKGLQTLAELNTKTEEIDVKKLKSYHQIILVRNQAGLKNLYKLISYGHLKYFYRKPLLPKSVLQEHREGLIFGSACEAGELFRALVENRPHEEIVELAKFYDYLEIQPDLNNAFMMRNGTAANREELRDYNRAIVKLGEELNMPVCATCDVHFMDPGDAKFREILQAGQGYDDAKFQPPLYFRTTDEMLEEFSYLGKEKAYEVVVENTNRIADCIEHIRPIPKGTFTPNIDGAEEDLTRITNEKAKEIYGDPLPELVEKRLNRELGSIIKHGFAVLYIIAQKLVWDSVAHGYEVGSRGSVGSSFVASMAGISEVNPLAPHYVCPKCKHSEFITDGSVGSGFDLPAKKCPKCGTEMNRDGHDIPFETFLGFDGDKAPDIDLNFSSEYQSSAHRYTEELFGRDNVFKAGTIAAVADKTAYGYVKKYLDERGITCSPTEINRLSIGCTGIKRTTGQHPGGMVVVPNDYDVYDFTPVQHPADSADSDVITTHFDFHSLHDTILKLDELGHVVPTMYRHLENITGLDIREVPTTDPRVIRMCTHAEELGVSGKDIYCKTGSLGIPEMGTGFTIQMLLDSNPTKFSDFLQVSGLSHGTDVWLGNAKDLIDNGTCTISDVIGTRDSIMTYLLHKGVEPKQAFQIMEDTRKGKAPKTFTPERIQMLKDHNVPDWYIDSCLKIKYMFPKAHAAAYVTAAIKLGWFKLYRPLAYYAVYFSTRGDDFDVELVMQGKEAVHNRIEELREKGNERTKKESDQLDLLYIINEMLCRGLDFLPIDLYRSHASQYLVEDEKIRIPFSAISGIGLSAANALYEAAQKRDFISIEEFQNRSGASKTVIETLEKLNAFGNLPKSSQMTLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5VKQ5</accession>
    <name>R5VKQ5_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH merR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01822</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000186">
        <property type="protein sequence ID" value="CCZ84096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01822"/>
    <dbReference type="EnsemblBacteria" id="CCZ84096">
        <property type="protein sequence ID" value="CCZ84096"/>
        <property type="gene ID" value="BN566_01822"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000551">
        <property type="entry name" value="MerR-type_HTH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13411">
        <property type="entry name" value="MerR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00422">
        <property type="entry name" value="HTH_MERR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50937">
        <property type="entry name" value="HTH_MERR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH merR-type" evidence="1">
        <location>
            <begin position="3"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="138" mass="16175" checksum="3D3B252C929DBF81" modified="2013-07-24" version="1">MFVITIKEVSERYDLSQDTLRYYERVGMIPPVHRTASGIRDYQPDDLGWIELAKCMRGAGLPVEAIIEYVKLTQEGEHTIPARLQLLTEQLPARLQLLTEQRELLLEQKEKIDTTLKRLNYKIERYEIAVETGELKWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>R5VKU1</accession>
    <name>R5VKU1_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cyclic-di-AMP phosphodiesterase</fullName>
            <ecNumber evidence="1">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_01866</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Has phosphodiesterase (PDE) activity against cyclic-di-AMP (c-di-AMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3',3'-c-di-AMP + H2O = 5'-O-phosphonoadenylyl-(3'-&gt;5')-adenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54420"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:71500"/>
            <dbReference type="ChEBI" id="CHEBI:138171"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="2">For phosphodiesterase activity, probably binds 2 Mn(2+) per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GdpP/PdeA phosphodiesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="1"/>
    <dbReference type="EMBL" id="CBAR010000192">
        <property type="protein sequence ID" value="CCZ84141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01866"/>
    <dbReference type="EnsemblBacteria" id="CCZ84141">
        <property type="protein sequence ID" value="CCZ84141"/>
        <property type="gene ID" value="BN566_01866"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106409">
        <property type="term" value="F:cyclic-di-AMP phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014528">
        <property type="entry name" value="GdpP/PdeA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF026583">
        <property type="entry name" value="YybT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="SSF64182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0464">Manganese</keyword>
    <keyword evidence="1 3" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="37"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="DHH" evidence="4">
        <location>
            <begin position="345"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="DHHA1" evidence="5">
        <location>
            <begin position="522"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="351"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="355"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="357"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="423"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="447"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="502"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR026583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR026583-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ84141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="655" mass="73063" checksum="3971FC75A98CAE54" modified="2013-07-24" version="1">MKKKLRHILLLITGILTLLSIAVPLVATTLLYGSCRTALLFVPALLLSILFVVQLFFVFRRRYPLNRLRTDMSSAETPVLEQLTVPVVLVQENGDVFWYNTQFAKQILQDAGKGKQLIQRLLHLHTYAAQQQDQIVEFQDRSYKIHSMSCAAQKDTPALHAFQFNDVTELLQWKRDFASAKPCVLILVIDSYDDILQYAKESEKAQVSAEVEKVLESFMTGTNGIIRKIENDMFYAVVETRHLRVMMQEKFKVLDDARKILVNGRIHITFSIGVGFGANSLEESEKFAHQSLDMALGRGGDQAAVKTENGFCFFGGTSKGVEKKSKTKIRSVATALQELIENADQIFLMGHRFGDLDAIGSACGLAGAIDMMKKPVSIVVNQKNCLANQLIQYMKKSERPPEFIEPEEAMERVSSNSLLIVVDTHNKDILESVELYEACRYVAVIDHHRKNVNFIENAVLFHHEPYASSASEMVTEIIQYFRLDGEIPAANADALLAGITLDTKNFVMRTGVRTFEAAAYLRKIGADTIQVKSLFSSTISMYQMRSQIVAHAEIYHKSAISAVKMQDADTRVLAAQAADELLSIQGVEASFVLFPDQNGFSISARSLGGMNVQLIMEELGGGGHQTMAATQIAGVTLQEAKERLLQVLEAYEEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>R5VKX0</accession>
    <name>R5VKX0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN566_01901</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000193">
        <property type="protein sequence ID" value="CCZ84176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01901"/>
    <dbReference type="EnsemblBacteria" id="CCZ84176">
        <property type="protein sequence ID" value="CCZ84176"/>
        <property type="gene ID" value="BN566_01901"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05398">
        <property type="entry name" value="NT_ClassII-CCAase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032810">
        <property type="entry name" value="CCA-adding_enz_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002646">
        <property type="entry name" value="PolA_pol_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032828">
        <property type="entry name" value="PolyA_RNA-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01743">
        <property type="entry name" value="PolyA_pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12627">
        <property type="entry name" value="PolyA_pol_RNAbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13735">
        <property type="entry name" value="tRNA_NucTran2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PolyA_pol" evidence="6">
        <location>
            <begin position="26"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="PolyA_pol_RNAbd" evidence="7">
        <location>
            <begin position="174"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA_NucTran2_2" evidence="8">
        <location>
            <begin position="300"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="368"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ84176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="457" mass="52093" checksum="46D234E61DFD48C5" modified="2013-07-24" version="1">MNEQITIPADGIWILNRLQEHGYVGYAVGGCVRDALLHRTPHDWDFCTSATPEQIQACFSDVPCLTIGSQHGTIGVLRNHTVYEITTFRKETTYHDHRHPDAVQFADSLQTDLQRRDFTINAMAYHPETGIIDPFHGAADLQKRQLRCVGVPTERFTEDALRILRAVRFAAVYGLTIQPETAAAMERTAPFLKWVSAERILSECDRIVTAQYAAATLAAYCSIWFRLFPEMQPLQGFQQYHPRHAYDVWEHSLLALSHSDQQDTVLCWAALLHDIGKPACFHRDAAGIGHFYGHPAESAALAKQICKRLKMDRKRTDAIVALITAHEVQLLPQRSYLLRMLRKYGAVQAKRLLTLRKADLQAQVPAYAEQDLQKLEQMQQLLQELLQEQACFQLKDLAIRGNDLLAMGIPKGVLIGKLLQDALEQVIRGKLPNEREALLQWVQERWNRRKQHQNPTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5VKY7</accession>
    <name>R5VKY7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Teichoic-acid-transporting ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_01921</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01921"/>
    <dbReference type="EnsemblBacteria" id="CCZ84196">
        <property type="protein sequence ID" value="CCZ84196"/>
        <property type="gene ID" value="BN566_01921"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03220">
        <property type="entry name" value="ABC_KpsT_Wzt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015860">
        <property type="entry name" value="ABC_transpr_TagH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="24"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="258"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="61"/>
            <end position="68"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ84196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="430" mass="48637" checksum="1834E80F14D24301" modified="2013-07-24" version="1">MEPIIDASHVYIRYLLGDFRDIGLKEWTIQKVKRERRVESRWSVEDVSFTLEKGDFLGIIGANGAGKSTILKAICGILPPAKGSIQVNGNISALLELGTGFDSDLNLIENIYMRGALLGYEKNFITERIDEIIEFAELKEFQFYKYKQLSSGMRSRLAFAIACMVDPEILILDEVLSVGDGGFRRKSEQKMMEVIQSGATTLFVSHSVAQMKRLCNKVLWLDMGKQIAFAKNKQESEKILTCYSNYMKERAINPAAKPSLTLPAPAKKTEKPTAPKKPATKPTPVAKPQKRLASDIQYEIYSKAYIDILNEMVLHPETVDQFKAYFQDKHITHLAIYGESMVLKTIAVLLKDAGVSVDYLLENGKKSSEYAKTIHSRDIHKYPDTQCILIGDVRFEKNILEKVHKRTKIPVITISEAMHYKKDSKKNTTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VL16</accession>
    <name>R5VL16_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01956</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84231">
        <property type="protein sequence ID" value="CCZ84231"/>
        <property type="gene ID" value="BN566_01956"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="98" mass="11732" checksum="8F3D4BD0DFDD0B37" modified="2013-07-24" version="1">MMFMNGEYVKTIEDLKRCLSLEELVYNYYSGELEIWLRKIGETEKADQLQHIQKDNAYLLVQLYAVFGWNPELSDEEIRRSEDFSDRMQREKIASIES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5VLA2</accession>
    <name>R5VLA2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Asparagine synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN566_00234</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the asparagine synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000213">
        <property type="protein sequence ID" value="CCZ84321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00234"/>
    <dbReference type="EnsemblBacteria" id="CCZ84321">
        <property type="protein sequence ID" value="CCZ84321"/>
        <property type="gene ID" value="BN566_00234"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004066">
        <property type="term" value="F:asparagine synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006529">
        <property type="term" value="P:asparagine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01991">
        <property type="entry name" value="Asn_Synthase_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00712">
        <property type="entry name" value="AsnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006426">
        <property type="entry name" value="Asn_synth_AEB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001962">
        <property type="entry name" value="Asn_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033738">
        <property type="entry name" value="AsnB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00733">
        <property type="entry name" value="Asn_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001589">
        <property type="entry name" value="Asn_synthetase_glu-h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01536">
        <property type="entry name" value="asn_synth_AEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0061">Asparagine biosynthesis</keyword>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 5" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="8">
        <location>
            <begin position="2"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="5">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="355"/>
            <end position="356"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for beta-aspartyl-AMP intermediate formation" evidence="7">
        <location>
            <position position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ84321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="629" mass="71813" checksum="4D69AE2EFBA2D5AE" modified="2013-07-24" version="1">MCGFTGFTNHIDNSNAVLQEMLNRIKHRGPDAEGTYIDEDIALGHRRLSIIDVSDAGTQPLYSADGNLALLFNGEIYNYKTIRQALIEKGYQFATQTDSEVLIYGYQEYGTALLQKLRGMFSFVIWNKKEKTLFGARDFFGIKPLYYAQMGETFLFGSEIKSFLPHPHFKKVLNTAALENYLTFQYSPCTETFFKGVYKLPPAHYFIYKNGKLDIQQYWEIEFHADEKPSLDDWVNQISDTFHNSVEAHKIADVEVGSFLSSGVDSSYVAAIADVDKTFTVGFGKEERYNEIGWAKGFSEAIGKKNYSKVIEPEEYWAHLPMIQYHMDEPLADPAAVALYFVCNLASQSLKVVLSGEGADEIFGGYNVYSDPGSTSYEKLPRGLRRGIMHIAESLPAHRGVNFFVRKGKDLEERFIGNAYMFTPKDRKALLKIHTNAPDPMAITKPYYDQVKDQDDVTKMQYLDLHLWMAGDILLKADKMSMANSLELRVPFLDKEVMALAQRIPTRYRVIHKPSESGGTPYITKYAMRLAAKKDTPPQTAKTAAKKKLGFPVPIRVWLKEDTYYNMVKNTFEGNAAQQFFHTEKLVQLLDDHRAGKADNSRKIWTVYMFLVWYHVYFESDVIPTKPNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>R5VLI5</accession>
    <name>R5VLI5_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="2">PPIase</shortName>
            <ecNumber evidence="2">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00313</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 2">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="2"/>
    <dbReference type="EMBL" id="CBAR010000224">
        <property type="protein sequence ID" value="CCZ84406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00313"/>
    <dbReference type="EnsemblBacteria" id="CCZ84406">
        <property type="protein sequence ID" value="CCZ84406"/>
        <property type="gene ID" value="BN566_00313"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000413">
        <property type="term" value="P:protein peptidyl-prolyl isomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044666">
        <property type="entry name" value="Cyclophilin_A-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625">
        <property type="entry name" value="PTHR45625"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="SSF50891"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="3">
        <location>
            <begin position="47"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="250" mass="26857" checksum="2C6347C87F6E9D6B" modified="2013-07-24" version="1">MKQIPALLLSSVLLLEGLTGCGSQQETAVKNFTAPEQGEELVVIHVKDYGTIKIKVFSDLLPKACENFVTHAKDGYYDGLPFHRVIENFMIQGGDPKGDGTGGESIWGGKFDGGVDDSLIHVRGAVAYANSGGTDTDGSQFYIVTGGAVSMDTLKQYEEHGDGSYTKEQKELYTTIGGAPWLDGKYTVFGQVVDGLDVAITISEQETDSNNKPYTSIIMDSVTVEPYDGSELHWYASSATEPSETSDDAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>R5VLK5</accession>
    <name>R5VLK5_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 10">Adenylosuccinate synthetase</fullName>
            <shortName evidence="8">AMPSase</shortName>
            <shortName evidence="8">AdSS</shortName>
            <ecNumber evidence="8 10">6.3.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">IMP--aspartate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">purA</name>
        <name evidence="11" type="ORF">BN566_00333</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>GTP + IMP + L-aspartate = GDP + 2 H(+) + N(6)-(1,2-dicarboxyethyl)-AMP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15753"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="6.3.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8 10">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 10">Belongs to the adenylosuccinate synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.4" evidence="8 10"/>
    <dbReference type="EMBL" id="CBAR010000225">
        <property type="protein sequence ID" value="CCZ84426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00333"/>
    <dbReference type="EnsemblBacteria" id="CCZ84426">
        <property type="protein sequence ID" value="CCZ84426"/>
        <property type="gene ID" value="BN566_00333"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00075">
        <property type="reaction ID" value="UER00335"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004019">
        <property type="term" value="F:adenylosuccinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03108">
        <property type="entry name" value="AdSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.170.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00011">
        <property type="entry name" value="Adenylosucc_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018220">
        <property type="entry name" value="Adenylosuccin_syn_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033128">
        <property type="entry name" value="Adenylosuccin_syn_Lys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042109">
        <property type="entry name" value="Adenylosuccinate_synth_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042110">
        <property type="entry name" value="Adenylosuccinate_synth_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042111">
        <property type="entry name" value="Adenylosuccinate_synth_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001114">
        <property type="entry name" value="Adenylosuccinate_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11846">
        <property type="entry name" value="PTHR11846"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00709">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00788">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00184">
        <property type="entry name" value="purA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01266">
        <property type="entry name" value="ADENYLOSUCCIN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00513">
        <property type="entry name" value="ADENYLOSUCCIN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 8 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 8 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 8 10" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="IMP binding" evidence="8">
        <location>
            <begin position="13"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="region of interest" description="IMP binding" evidence="8">
        <location>
            <begin position="38"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="298"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="13"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="41"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="12"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="40"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="330"/>
            <end position="332"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="413"/>
            <end position="415"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ84426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="424" mass="46108" checksum="9A8946815945F946" modified="2013-07-24" version="1">MPANIIVGSQWGDEGKGKIIDIMASRAEVVVRATGGNNAGHTVVNHGEVYKLHLIPSGILYPDTLCLIGAGVVLDPKDFISELDNLESRGISTANLKIDPRAHVVMPWHIELDGLSEKFRGNSDIGTTKRGIGPTYMDKYERCGLRMYDLVHPEIFAQKARTTGELKNKIITAVYGGTAFDLDAVIAEYIEYGKRLAPYIADVSVLTYEADKAGKTIMFEGAQATLLDIDFGTYPYVTSSHPISAGACIGSGVGPKMITNIIGVAKAYTTRVGKGPFPTELNDETGDLIRNKGGEFGTTTGRPRRTGWFDAVIVRHSVRTNGLDGLAINKLDTLSGLKTLKMCVGYEKPDGTILKDFPPALEELADCKPIYEEFEGFSEDISSCKTFDELPEACKKYIARLEEVCGCKVVMVGNGPDRSQILER</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>R5VMA3</accession>
    <name>R5VMA3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphocarrier protein HPr</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_00529</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The phosphoryl group from phosphoenolpyruvate (PEP) is transferred to the phosphoryl carrier protein HPr by enzyme I. Phospho-HPr then transfers it to the PTS EIIA domain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000267">
        <property type="protein sequence ID" value="CCZ84676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00529"/>
    <dbReference type="EnsemblBacteria" id="CCZ84676">
        <property type="protein sequence ID" value="CCZ84676"/>
        <property type="gene ID" value="BN566_00529"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00367">
        <property type="entry name" value="PTS-HPr_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1340.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000032">
        <property type="entry name" value="HPr-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035895">
        <property type="entry name" value="HPr-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001020">
        <property type="entry name" value="PTS_HPr_His_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002114">
        <property type="entry name" value="PTS_HPr_Ser_P_site"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00381">
        <property type="entry name" value="PTS-HPr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00107">
        <property type="entry name" value="PHOSPHOCPHPR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55594">
        <property type="entry name" value="SSF55594"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01003">
        <property type="entry name" value="PTS_HPr_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51350">
        <property type="entry name" value="PTS_HPR_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00369">
        <property type="entry name" value="PTS_HPR_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00589">
        <property type="entry name" value="PTS_HPR_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0598">Phosphotransferase system</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HPr" evidence="6">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ84676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="90" mass="9935" checksum="8B84BC210BCE0A51" modified="2013-07-24" version="1">MYSKDVMVQNQVGLHARPATFFIQKANEFRSSIWIEKEERRVNAKSLLGILSLGIIGGTQIKIIADGADEQAAVNALVELVESGFSEETR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5VQJ3</accession>
    <name>R5VQJ3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00657</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000012">
        <property type="protein sequence ID" value="CCZ82852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82852">
        <property type="protein sequence ID" value="CCZ82852"/>
        <property type="gene ID" value="BN566_00657"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ82852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="602" mass="67237" checksum="81EEFB839DE59DA8" modified="2013-07-24" version="1">MDKKQCERMFEQMQPPKAVVQRLQQRIHAEEAASLAEQQPNRSIQTLHTTTAPPPPKRIRCSWQKLSGGVVAAVFLLSIGTVIGYHLKHDEQVFVPSSESTTEASETPKATTQSLVLQYQGQNLREMAVVNPDRVEVQDTLGEGSLMGVDGATTVSVYQVYLKDTATIEESVVAAEWNGTQYLFVSVPDVVRTFKDYPIIENWISSGSFTFLDEDQITERTYENYTNEELSPLFTFLQNNADSINFSSGLSSDADVPEEQVQMTDRFTWDYAITISSDCLDFSGVLYFDVGDGVWKMDLLDRQAYFLSTPEILTEMLSEVAASHAVVPNTQNGNASENSTSATGELLGRTAIDGSNPKNTTIQDLQPQLLNQPFSFQQVSGSLVDSYTGMSSDGTGAIEEIRSLTTFQIDLATRTKDVLRTVESPPDSQSDSSETATWEYIYANQQKLSMEQVWTANNWYTIYPDLQCYTVLNAGTDEWTNYDTAVNMLADGTVFEDGIFYLGQEFSARSEDLEGVVVPQNLFVFGTVSYLNRDCYLVGKYEDGSITRYLIDMEQLIALKRESVGADFQKIDYFKEILFDEEAVPVPEVEESSLTAGMQYLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VQU0</accession>
    <name>R5VQU0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00727</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000029">
        <property type="protein sequence ID" value="CCZ82927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82927">
        <property type="protein sequence ID" value="CCZ82927"/>
        <property type="gene ID" value="BN566_00727"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ82927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="108" mass="12160" checksum="8679438D4844CDDF" modified="2013-07-24" version="1">MKSSSRSRSKQAVTYMEVYGANRVLYRVTASNAHRNQKLSVVYGIFLQDLRSGETAEIHAFSNSLEKTVCFVNDLIQKQVQPYQVYNAALRQLSLEVPFLQNGSILGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>R5VR47</accession>
    <name>R5VR47_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC-type multidrug transport system ATPase and permease components</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN566_00795</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000038">
        <property type="protein sequence ID" value="CCZ83002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00795"/>
    <dbReference type="EnsemblBacteria" id="CCZ83002">
        <property type="protein sequence ID" value="CCZ83002"/>
        <property type="gene ID" value="BN566_00795"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="PTHR24221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="140"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="168"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="258"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="27"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="345"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="378"/>
            <end position="385"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ83002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="585" mass="65572" checksum="DCF05A42D44CF248" modified="2013-07-24" version="1">MPKKTAKEPQNWKRIWQMIRPYRGWMIASMVLAAFTVAFTLYIPILVGDAIDAMVEAGKVSFAIITPILWKIAILAVLTALFQWVMQTMHNKITYQVVRDFRTAAFEKIQKLPLSYLDRHPTGETVSRVITDVDQFADGLLMGFTQLFTGVFTLIGTLLFMLVLDWKISLVVILMTPLSILFAKSLSGHIYKMFRAQSEARAEQTAMVNEVIGNQKVIQAFHHEEATMEQFQENNKKLQETSLRASFFSSLINPGTRFIYAVVYAAVGLIGALSVLSGRITIGGLSCFLNYTNQYTKPFNEITGVLTELQNAVACANRLFNLLDEPEETPDAPDAVDRKTVDGNVTIQDVDFSYVPEKPLIEHLNLQAKSGQRIAIVGPTGCGKTTLINLLMRFYEVNSGAILIDGVETKHMTRHSLRQNFGMVLQETWLKSGTIRENICMGKPDATEEEMILAAKAAHAHTFIQQLPQGYDTEIRDGGNLSQGQKQLLCIARVMLCLPPMLILDEATSSIDTRTEVRIQEAFATMMKGRTSFIVAHRLSTIREADVILVMKDGHIIEQGNHETLLEKNGFYAKLYRSQFVQTAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>R5VRE2</accession>
    <name>R5VRE2_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Orotidine 5'-phosphate decarboxylase</fullName>
            <ecNumber evidence="7">4.1.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">OMP decarboxylase</fullName>
            <shortName evidence="7">OMPDCase</shortName>
            <shortName evidence="7">OMPdecase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pyrF</name>
        <name evidence="9" type="ORF">BN566_00914</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>H(+) + orotidine 5'-phosphate = CO2 + UMP</text>
            <dbReference type="Rhea" id="RHEA:11596"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57538"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="EC" id="4.1.1.23"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the OMP decarboxylase family. Type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.23" evidence="7"/>
    <dbReference type="EMBL" id="CBAR010000061">
        <property type="protein sequence ID" value="CCZ83127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00914"/>
    <dbReference type="EnsemblBacteria" id="CCZ83127">
        <property type="protein sequence ID" value="CCZ83127"/>
        <property type="gene ID" value="BN566_00914"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00120"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004590">
        <property type="term" value="F:orotidine-5'-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01215">
        <property type="entry name" value="OMPdecase_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018089">
        <property type="entry name" value="OMPdecase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011995">
        <property type="entry name" value="OMPdecase_type-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001754">
        <property type="entry name" value="OMPdeCOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43375">
        <property type="entry name" value="PTHR43375"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00215">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00934">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02127">
        <property type="entry name" value="pyrF_sub2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00156">
        <property type="entry name" value="OMPDECASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="6 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 7" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OMPdecase" evidence="8">
        <location>
            <begin position="16"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ83127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="306" mass="32702" checksum="531C4CD2B1604083" modified="2013-07-24" version="1">MAFDRLIKRIQETGNPSVAGLDPKLDYVPAFIREKHFAADGMTLKAAANAIKEFNFALIDALADVVPAIKPQAAYYEMYGYEGVRVLAETIAYAQSKHLFVITDGKRNDIGATMQAYATAHLGSVTVGTETLEPFGADALTVNGYLGTDGIRPLLDICAERDKGIFVLVKTSNPSSGELQDQKIGENTVYDVMGDMCETWGAEQIGASGYSAVGAVVGATYPEQLKELRVRLPHTFFLVPGYGAQGGGAAGVAAAFDQNGLGAIVNSSRGIMCAYQKEADCPAEDFAGAARREAIRMREDLCSHIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5VRJ1</accession>
    <name>R5VRJ1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00960</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000069">
        <property type="protein sequence ID" value="CCZ83177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00960"/>
    <dbReference type="EnsemblBacteria" id="CCZ83177">
        <property type="protein sequence ID" value="CCZ83177"/>
        <property type="gene ID" value="BN566_00960"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="463" mass="51096" checksum="C6B44346EE6FB5F3" modified="2013-07-24" version="1">MKTAKNPEKKRFRLFSYLFNTRMGTMIFSIITAIFLWFAISINLYPTTPRTISHIPLTVDIAGTAAELNGYSVIDYSVKEVTVQIEGNRSQVGNLDANDMIATPVVENVTSSGVKTLDISVSSKDGTQFTVKSISPKSVNVRFDKMVTKKYPIQGDIGKITSSSTCVINTEDVKVTPNEVEVTGPETIMNRISYAAAVIPKERELSATTILTTDQLEFRDENDTPVESTYLEYTLSNYAVEIPVQYTQTLDITYQLRNVPTNFDEAYLRSLLKQSEDHITLATSDANLDAETAFSIGQISLSDIGIGYSKDFPLEIPENYENLSGVTSVNLALDASNLVEKEFVVTDISIMNAPTTYNMTVDTSQLTVKMIGPKNQIEHLSAADLTVTVNLLGAPQMEGESVSFSYTPTISCEKWDRVWAVGDYTNKVYVKGVKVDTPVVTTTVTTPERVTVHTTETTTTTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>R5VRL4</accession>
    <name>R5VRL4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3 4">Single-stranded DNA-binding protein</fullName>
            <shortName evidence="2">SSB</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_00980</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000070">
        <property type="protein sequence ID" value="CCZ83197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00980"/>
    <dbReference type="EnsemblBacteria" id="CCZ83197">
        <property type="protein sequence ID" value="CCZ83197"/>
        <property type="gene ID" value="BN566_00980"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04496">
        <property type="entry name" value="SSB_OBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00984">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000424">
        <property type="entry name" value="Primosome_PriB/ssb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011344">
        <property type="entry name" value="ssDNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10302">
        <property type="entry name" value="PTHR10302"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00436">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002070">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00621">
        <property type="entry name" value="ssb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50935">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="128"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="128"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR002070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ83197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="179" mass="19840" checksum="843B0B0466FE3DD3" modified="2013-07-24" version="1">MLNKVILMGRLCADPDFRQTQSGIAVCRIRVAIDRPYTSKSTGERQADFINVVCWRQQAEFVSRYFHKGSMIIVEGRLQNSDYTDGNGVKHYSMEVQADNVTFGETRAAAQANGNYTAPSNQSGYQYQTQYQQQPSAPQAAPQQPAAAPAPQQQNNSVPIQLGDLSDFEEILSDGEVPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5VRP9</accession>
    <name>R5VRP9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein (Competence-and mitomycin-induced)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01013</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000080">
        <property type="protein sequence ID" value="CCZ83232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01013"/>
    <dbReference type="EnsemblBacteria" id="CCZ83232">
        <property type="protein sequence ID" value="CCZ83232"/>
        <property type="gene ID" value="BN566_01013"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036653">
        <property type="entry name" value="CinA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008136">
        <property type="entry name" value="CinA_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02464">
        <property type="entry name" value="CinA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142433">
        <property type="entry name" value="SSF142433"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00199">
        <property type="entry name" value="PncC_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CinA" evidence="2">
        <location>
            <begin position="24"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="8"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="189" mass="20092" checksum="5122B1388DEE9929" modified="2013-07-24" version="1">MIKQRYDFDEVRKIYQENQKQLDNLVQNIVQLSCENGQTIATAESCTGGLLSQKITAVPGASQMFALGICTYTNEMKTAILHVPESTLHTYTAVSPQTAIAMANGVAALSHADLALSVTGYAGPGGGTSEHPVGTVFLGVAYHGTAFAVHLPFDQLGVSTREGVRQGTAVCAFGIAREILMEESVCRNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5VSI5</accession>
    <name>R5VSI5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cohesin domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01256</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000117">
        <property type="protein sequence ID" value="CCZ83487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83487">
        <property type="protein sequence ID" value="CCZ83487"/>
        <property type="gene ID" value="BN566_01256"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008965">
        <property type="entry name" value="CBM2/CBM3_carb-bd_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49384">
        <property type="entry name" value="SSF49384"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004407356" evidence="2">
        <location>
            <begin position="38"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="344" mass="35316" checksum="508A9568974D1B19" modified="2013-07-24" version="1">MKSKTTKGIAVLLACFLGMAAVGCSGDNDSSSKNSDAAEVSAATLPVETDAQGEAVTDASGNEVLASGATDFMVKEIEFTWGNQNGSSDSSQDATEATDSNGNAVTTAAGGSSNAATPANGNASNGGGNNASNGNSASNGNSEVVTSYAAVTDDSGNKVVDDNGKVVTEVVTTPSSNGGAVVDPNQNAASSYTEKLDTFQAYWMDMTKSQDIVFNGDMINVTFKVKETTPDGNYVIHLSDSQYNSEFANWDAKTLVPSVTNGCITVGDATPEAQTATNASDFVLTADSVTAKQGDEVTVSFRIADNPGMVAFVLRFQYDCNALEIVSAEVGDDCADALRNGLNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5VSJ2</accession>
    <name>R5VSJ2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Amine oxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01261</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000117">
        <property type="protein sequence ID" value="CCZ83492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01261"/>
    <dbReference type="EnsemblBacteria" id="CCZ83492">
        <property type="protein sequence ID" value="CCZ83492"/>
        <property type="gene ID" value="BN566_01261"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="556" mass="63622" checksum="CF04408081BDF607" modified="2013-07-24" version="1">MNLFGNPQKINRDLQFFCEASPKLKNVYQIKEGITIIKNVVIIGAGPAGLTAAYELLQRAPKEYSITILEESNAIGGISKTVKYKNNRMDIGGHRFFSKDQQVMDWWQKMMPRQGKPSFDDIKLHREKKLAAGGPDPEKTDRVMLVRNRVSRIYYKHKFFDYPVSLKWDTIKNMGFATTVAAGCSYLKSTIGKKEESSLENFYINRFGKKLYSMFFEGYTEKLWGRHPREISADWGSQRVKGLSITAVIKDMFSKLNPNKTNRKVETSLIEEFYYPKYGPGQLWEITASEIEKMGGKILMNSEVTQIHTDKTGKILSLTYKQDGKEQEIAGDLFISSMSLKDLVAGMNDVPNNIAAIAKGLPYRDFVTVGLLVDKLNLKNETNIPTLGNIVPDCWIYVQDVGVKLGRIQIFNNWSPYMVEDPEHTVWIGLEYFCTEGDSFWNMDDKACINMAIKELVSMGIISSPSDVKDSHREKVKKAYPAYFDTYAQIDDLIAYLNQFENLYCVGRNGQHRYNNMDHSMATSFEAVDNIISGKKDKKNIWSVNTEKEYHEEKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>R5VSP6</accession>
    <name>R5VSP6_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nicotinate-nucleotide diphosphorylase (carboxylating)</fullName>
            <ecNumber evidence="4">2.4.2.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Quinolinate phosphoribosyltransferase [decarboxylating]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BN566_01316</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the catabolism of quinolinic acid (QA).</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from quinolinate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the NadC/ModD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.19" evidence="4"/>
    <dbReference type="EMBL" id="CBAR010000122">
        <property type="protein sequence ID" value="CCZ83547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01316"/>
    <dbReference type="EnsemblBacteria" id="CCZ83547">
        <property type="protein sequence ID" value="CCZ83547"/>
        <property type="gene ID" value="BN566_01316"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00331"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004514">
        <property type="term" value="F:nicotinate-nucleotide diphosphorylase (carboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01572">
        <property type="entry name" value="QPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004393">
        <property type="entry name" value="NadC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027277">
        <property type="entry name" value="NadC/ModD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036068">
        <property type="entry name" value="Nicotinate_pribotase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037128">
        <property type="entry name" value="Quinolinate_PRibosylTase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002638">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022412">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32179">
        <property type="entry name" value="PTHR32179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01729">
        <property type="entry name" value="QRPTase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02749">
        <property type="entry name" value="QRPTase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006250">
        <property type="entry name" value="NadC_ModD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51690">
        <property type="entry name" value="SSF51690"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00078">
        <property type="entry name" value="nadC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="QRPTase_N" evidence="12">
        <location>
            <begin position="21"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="QRPTase_C" evidence="11">
        <location>
            <begin position="111"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="10">
        <location>
            <begin position="132"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="10">
        <location>
            <begin position="240"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="10">
        <location>
            <begin position="261"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR006250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR006250-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CCZ83547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="282" mass="30684" checksum="7106C51A5F8448FB" modified="2013-07-24" version="1">MLLQPFYIDKIITTALEEDIHYVDVTTDYLLDDEHTSEAYYLAKDTGVLCGIDIAKRVFELAGGNVTMEILKKDGETVQKGDILARMKGSTKTLLKGERTALNLLQHMSGIATATAECVALVAGTRAQITDTRKTLPGLRQLQKYAVTVGGGKNHRYNLSDGAMLKDNHIDAYGSITGAVTALRKKIGHMVKIELEVRNLDELREALAAGADIIMLDNMSCDEMKKAVAINDGKVLLEASGNVTKDNIRSVAETGVDIISLGALTHSVKCFDISMRIQTVKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>R5VSR2</accession>
    <name>R5VSR2_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pyridoxal phosphate homeostasis protein</fullName>
            <shortName evidence="1">PLP homeostasis protein</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_01335</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Pyridoxal 5'-phosphate (PLP)-binding protein, which is involved in PLP homeostasis.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the pyridoxal phosphate-binding protein YggS/PROSC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000131">
        <property type="protein sequence ID" value="CCZ83567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01335"/>
    <dbReference type="EnsemblBacteria" id="CCZ83567">
        <property type="protein sequence ID" value="CCZ83567"/>
        <property type="gene ID" value="BN566_01335"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02087">
        <property type="entry name" value="PLP_homeostasis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001608">
        <property type="entry name" value="Ala_racemase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011078">
        <property type="entry name" value="PyrdxlP_homeostasis"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10146">
        <property type="entry name" value="PTHR10146"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01168">
        <property type="entry name" value="Ala_racemase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004848">
        <property type="entry name" value="YBL036c_PLPDEIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="SSF51419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00044">
        <property type="entry name" value="TIGR00044"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ala_racemase_N" evidence="4">
        <location>
            <begin position="13"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1 2">
        <location>
            <position position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR004848-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="231" mass="25973" checksum="5B15984FC0F809F0" modified="2013-07-24" version="1">MENSFDYITENYNRIYNEIEAAKAKYRKPSDHVDFMAVTKTVAPEAVNHAIHCGISLLGENRVQEYLSKRDFYDPSATVHFIGHLQTNKVKYIIEDMALIHSVDSVHLAKEIDRQAARVGKVQEILIEVNIGDETSKSGIAPEQLVPLLEEVATFQNLKVKGLMTIPPAGENEKFLSKMQDLYLDIRSKNMDNIDMDILSMGMSGDYAKAIQYGATLVRIGSALFGARVYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VSY3</accession>
    <name>R5VSY3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01383</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000147">
        <property type="protein sequence ID" value="CCZ83647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83647">
        <property type="protein sequence ID" value="CCZ83647"/>
        <property type="gene ID" value="BN566_01383"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="63" mass="7112" checksum="DA36E2FC7374209A" modified="2013-07-24" version="1">MKSNEKKDTLYLCDPQKNTECPKGNCQIPDGCFLTIKKEFAVTDKNGKPIIGIELHQEQSSQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5VT23</accession>
    <name>R5VT23_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Threonine synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_01423</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the threonine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000153">
        <property type="protein sequence ID" value="CCZ83687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01423"/>
    <dbReference type="EnsemblBacteria" id="CCZ83687">
        <property type="protein sequence ID" value="CCZ83687"/>
        <property type="gene ID" value="BN566_01423"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1380.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029144">
        <property type="entry name" value="Thr_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037158">
        <property type="entry name" value="Thr_synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004450">
        <property type="entry name" value="Thr_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14821">
        <property type="entry name" value="Thr_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00260">
        <property type="entry name" value="thrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thr_synth_N" evidence="6">
        <location>
            <begin position="6"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="PALP" evidence="5">
        <location>
            <begin position="108"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="4">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR604450-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF14821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ83687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="501" mass="55561" checksum="2893DB113ABEC127" modified="2013-07-24" version="1">MVVMYYKSTRDSSVRVESAVAISQGISADGGLFVPEQIPSITKEELAALADKTYAQRAAFVFSRYLTDFTEAELVYCTESAYNTKRFETENIAEIAHLFEGTYMLELWHGPTCAFKDMALQILPYFLTTAMKKLQLKEKIVILVATSGDTGKAALEGFKDVEGTEIMVFYPEDGVSDMQKRQMVTQEGSNVNVCAVKGNFDDCQSGVKKIFTDKTVKEELAAGNMRFSSANSINWGRLVPQIVYYVSSYVTLVQQGELQMGETLNVVVPTGNFGNILAAYYAKQMGVPLGKLICASNINKVLTDFINTGVYNRNREFYATCSPSMDILISSNLERLLYLLTGEDDAQIREWFTALSETGTYEVTDAVKTALQKDFFGGFCDDAATKATIHQVYETYGYTCDTHTAVAVKVYQDYRKATGDTTKTLIASTASPYKFSPSVLEAIAKEPVSGDEYAMVDQLHELSNLPIPAALADLKNKERRFTSCIDKNDLQSFVLQQLHLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5VTG8</accession>
    <name>R5VTG8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01562</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000165">
        <property type="protein sequence ID" value="CCZ83827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01562"/>
    <dbReference type="EnsemblBacteria" id="CCZ83827">
        <property type="protein sequence ID" value="CCZ83827"/>
        <property type="gene ID" value="BN566_01562"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13508">
        <property type="entry name" value="Acetyltransf_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="150" mass="17273" checksum="4296374137DB0229" modified="2013-07-24" version="1">MIEIQQVSEQKKQYLPLLLLGDEQESMIDRYLEQGTLYVMQKAGNPIAVCVILRASETEWEVKNLAVAVSEQGKGYGRKFLQFLEQQLAGTAEWLILGTGDSPKTIPFYQKCGFVPYRRIPHFFLEHYDHPMIEDGVLLDDMVYLRKSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>R5VU62</accession>
    <name>R5VU62_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 7">LL-diaminopimelate aminotransferase</fullName>
            <shortName evidence="7">DAP-AT</shortName>
            <shortName evidence="7">DAP-aminotransferase</shortName>
            <shortName evidence="7">LL-DAP-aminotransferase</shortName>
            <ecNumber evidence="4 7">2.6.1.83</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">dapL</name>
        <name evidence="9" type="ORF">BN566_01773</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in the synthesis of meso-diaminopimelate (m-DAP or DL-DAP), required for both lysine and peptidoglycan biosynthesis. Catalyzes the direct conversion of tetrahydrodipicolinate to LL-diaminopimelate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>(2S,6S)-2,6-diaminoheptanedioate + 2-oxoglutarate = (S)-2,3,4,5-tetrahydrodipicolinate + H(+) + H2O + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:23988"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57609"/>
            <dbReference type="EC" id="2.6.1.83"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 7">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (aminotransferase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. LL-diaminopimelate aminotransferase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.83" evidence="4 7"/>
    <dbReference type="EMBL" id="CBAR010000182">
        <property type="protein sequence ID" value="CCZ84047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01773"/>
    <dbReference type="EnsemblBacteria" id="CCZ84047">
        <property type="protein sequence ID" value="CCZ84047"/>
        <property type="gene ID" value="BN566_01773"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00466"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010285">
        <property type="term" value="F:L,L-diaminopimelate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033362">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate, diaminopimelate-aminotransferase pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01642">
        <property type="entry name" value="DapL_aminotrans_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019942">
        <property type="entry name" value="DapL/ALD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43144">
        <property type="entry name" value="PTHR43144"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03542">
        <property type="entry name" value="DAPAT_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="8">
        <location>
            <begin position="35"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="7">
        <location>
            <begin position="108"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="7">
        <location>
            <begin position="236"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="282"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="378"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="7">
        <location>
            <position position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ84047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="401" mass="44634" checksum="FA9835E319129D61" modified="2013-07-24" version="1">MPTINENFLKLEKNYLFINIAKKVNAFLKEHPEADLIRMGIGDVTLPIAPVCVEAMKKGADEMGVKETFKGYEDSGTGYDFLKQAIADYYQQYGVTLSLDEIRVNDGAKSDCGNIVDIFGDDNVVMVTDPAYPVYVDSNKMDGRTIIYANSNESNGFAAMPDFSVHADLIYLCSPNNPTGSVYTKEQLKTWIDYAKQNKAIIIYDAAYEAFITQPDIPHSIFEVEGARTCAIEMCSLSKTAGFTGMRCGYTVIPNELTVTASDGTVVSISQIWGRRQGSKFNGVSYPVQCAAAAVFTEEGRKQIQKNIAYYQENAAIISKTMDELGIPYTGGVNSPYIWFQCPNHMSSWEFFDEMLHKIAVVGTPGEGFGKNGDGWFRLTAFGDRERTKEAMERFKKMLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>R5VUA0</accession>
    <name>R5VUA0_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">GTPase Era</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">era</name>
        <name evidence="13" type="ORF">BN566_01818</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">An essential GTPase that binds both GDP and GTP, with rapid nucleotide exchange. Plays a role in 16S rRNA processing and 30S ribosomal subunit biogenesis and possibly also in cell cycle regulation and energy metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 9 10">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Era GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000186">
        <property type="protein sequence ID" value="CCZ84092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01818"/>
    <dbReference type="EnsemblBacteria" id="CCZ84092">
        <property type="protein sequence ID" value="CCZ84092"/>
        <property type="gene ID" value="BN566_01818"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04163">
        <property type="entry name" value="Era"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00367">
        <property type="entry name" value="GTPase_Era"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030388">
        <property type="entry name" value="G_ERA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005662">
        <property type="entry name" value="GTP-bd_Era"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42698">
        <property type="entry name" value="PTHR42698"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="SSF54814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00436">
        <property type="entry name" value="era"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51713">
        <property type="entry name" value="G_ERA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50823">
        <property type="entry name" value="KH_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 7 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5 7 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Era-type G" evidence="12">
        <location>
            <begin position="3"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="KH type-2" evidence="11">
        <location>
            <begin position="202"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="G1" evidence="9">
        <location>
            <begin position="11"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="region of interest" description="G2" evidence="9">
        <location>
            <begin position="37"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="G3" evidence="9">
        <location>
            <begin position="58"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="G4" evidence="9">
        <location>
            <begin position="120"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="G5" evidence="9">
        <location>
            <begin position="150"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="11"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="58"/>
            <end position="62"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="120"/>
            <end position="123"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CCZ84092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="299" mass="33788" checksum="7885716C1E7444B0" modified="2013-07-24" version="1">MTKTVFVTISGRTNVGKSSLLNAIVGERIASVSNKPQTTRTRITGIKTVGEIQMVFMDTPGLHKPKNKLSTYMLQAAKEAVNDMDVVLFVADCTRQPTEQELEFLQPLKASKTKVMLVLNKIDLLEEKNVLMQRIAEWNAAFPFAESFPISVSENDGVDLVEEAVCHCAIPAPHYFPDDKITDQPEQVLIAEMIREQVLRLLRDEVPHGVAVAVERLSERDDKDLLDIDATIYCERESHKGILIGKKGAMLKRIASQARAQLEQFFQIQVNLQCWVKVKEDWRNRDGLIRNFGLNNLDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>R5VUH3</accession>
    <name>R5VUH3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Radical SAM protein TIGR01212 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_01902</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01902"/>
    <dbReference type="EnsemblBacteria" id="CCZ84177">
        <property type="protein sequence ID" value="CCZ84177"/>
        <property type="gene ID" value="BN566_01902"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039661">
        <property type="entry name" value="ELP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032432">
        <property type="entry name" value="Radical_SAM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005911">
        <property type="entry name" value="YhcC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135">
        <property type="entry name" value="PTHR11135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135:SF1">
        <property type="entry name" value="PTHR11135:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16199">
        <property type="entry name" value="Radical_SAM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01091">
        <property type="entry name" value="uncharacterized_CHP01210-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01212">
        <property type="entry name" value="TIGR01212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Elp3" evidence="7">
        <location>
            <begin position="24"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ84177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="304" mass="34363" checksum="67E7F5B96EC8E607" modified="2013-07-24" version="1">MWDGGRRYYARFEYDQKQFHCRVMKAVVDGGFSCPNLDGTKGTGGCIFCDGGSGYFTHSGSIAEQLQTERTRIHAKFPTAKLIAYFQAHTNTYAPLSILKERYETALQQPDVIGLSIGTRPDCLPEDVLDYLEELSHRTVLTVELGLQTFQPKTAMLIHRCYENDIFLSAFSNLKARGIRTCVHLINGLPTETIEQMLDNARQLGQLQPDAVKLQLLHVIQDTPLEKWYQDGRLSTLDQETYVALIAEQLTYFPPETVIERLTGDGDARTLVAPLWSRNKRGVLNAIAAYQKSHNLVQGMRFSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>R5VUM7</accession>
    <name>R5VUM7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ATPases with chaperone activity ATP-binding subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_01952</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01952"/>
    <dbReference type="EnsemblBacteria" id="CCZ84227">
        <property type="protein sequence ID" value="CCZ84227"/>
        <property type="gene ID" value="BN566_01952"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001270">
        <property type="entry name" value="ClpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028299">
        <property type="entry name" value="ClpA/B_CS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041546">
        <property type="entry name" value="ClpA/ClpB_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17871">
        <property type="entry name" value="AAA_lid_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00300">
        <property type="entry name" value="CLPPROTEASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00871">
        <property type="entry name" value="CLPAB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="6">
        <location>
            <begin position="143"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="AAA" evidence="6">
        <location>
            <begin position="475"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="domain" description="ClpB_D2-small" evidence="7">
        <location>
            <begin position="646"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="61"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="355"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="61"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ84227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="741" mass="83429" checksum="BCF0B05A5767BE80" modified="2013-07-24" version="1">MVCAKELNIPQISEYMKHMGITDEELEQLSDQMMDMMDGDSFEMGGSGTLPPFLQNMMKNMPNMPLFSGDNQNEGEETLPSEDLTAEPPKPSKKEKRKKKKDLKFLNNYCTNLSQKAEEGKMDAIIGRDKEIERVVQILCRRTKNNPCLIGEPGVGKTAIAEGIAQRIAAGQVPFYLKGKQVYLLDLTSLVAGTQFRGQFESRIKGLVNEVKQEGNIILFIDELHNLVGAGNSEGSMNAANILKPALSRGEVQVIGATTFEEYRKYIEKDAALERRFQPVTVKEPTVEDTIEVLNGIKKYYEQHHRVHISDAMVRLCARLAERYITDRFLPDKAIDLMDESCACTCLRSPEISEYDTEKANLEQLEVKEKQMEEQTEEVDYEALAKLKGDMIRAKDRLAELQKKVDQVQVTEADLAKVIALWTGIPASKIQETEYTKLIALEDHLKARVIGQDEAVSALAKAIKRTRVQLTNKRRPASFIFVGPTGVGKTELVKVLSEELFDDNDPLIRLDMTEFMEKHAVARMIGSPPGYVGYDEAGQLTEKVRRKPYSVILFDEIEKAHPDVMNILMQILDEGKIDDAQGRTVSFANTIICMTSNAGSTDKSIGVGFNRTEEEVTKERAMKALREFLRPEFIGRIDEIIVFNKLTKDDYAKIAGLMLNEMKEPLLEKQITLNVTEDACKLIAEKAYGKTYGAREIRRVIRQEVEDQIAECIIHNANTLRALTITAKDGKLVVQDEEVIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVL5</accession>
    <name>R5VVL5_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 10">Adenylate kinase</fullName>
            <shortName evidence="8">AK</shortName>
            <ecNumber evidence="8 10">2.7.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">ATP-AMP transphosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">ATP:AMP phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Adenylate monophosphate kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">adk</name>
        <name evidence="12" type="ORF">BN566_00078</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>AMP + ATP = 2 ADP</text>
            <dbReference type="Rhea" id="RHEA:12973"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Purine metabolism; AMP biosynthesis via salvage pathway; AMP from ADP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8 10">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent ATP hydrolysis. Some bacteria have evolved a zinc-coordinating structure that stabilizes the LID domain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 9">Belongs to the adenylate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3" evidence="8 10"/>
    <dbReference type="EMBL" id="CBAR010000241">
        <property type="protein sequence ID" value="CCZ84532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00078"/>
    <dbReference type="EnsemblBacteria" id="CCZ84532">
        <property type="protein sequence ID" value="CCZ84532"/>
        <property type="gene ID" value="BN566_00078"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00588">
        <property type="reaction ID" value="UER00649"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:adenylate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044209">
        <property type="term" value="P:AMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01428">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00235">
        <property type="entry name" value="Adenylate_kinase_Adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006259">
        <property type="entry name" value="Adenyl_kin_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000850">
        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033690">
        <property type="entry name" value="Adenylat_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007862">
        <property type="entry name" value="Adenylate_kinase_lid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="PTHR23359"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05191">
        <property type="entry name" value="ADK_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00094">
        <property type="entry name" value="ADENYLTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01351">
        <property type="entry name" value="adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00113">
        <property type="entry name" value="ADENYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="5 8 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="ADK_lid" evidence="11">
        <location>
            <begin position="123"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="NMP" evidence="8">
        <location>
            <begin position="30"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="LID" evidence="8">
        <location>
            <begin position="122"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="10"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="57"/>
            <end position="59"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="85"/>
            <end position="88"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="132"/>
            <end position="133"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CCZ84532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="210" mass="22496" checksum="FC3B9DBE888D07E8" modified="2013-07-24" version="1">MNLILLGAPGAGKGTQAEVISEALSIPQISTGNMLREAVKNGTEYGLKAKAAMDSGALVSDEIVIGILKDRIAQPDCKNGFILDGFPRTVPQAEALDKMGVTIDKVLEIYVPDETIKERVSGRRVCEGCGASYHIKYKPTKVEGVCDKCGAATVIRKDDKPETVLDRLAVYHAQTEPLKDYYAKQGKLDTVVGQEELSDTTKLTLAAVGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVR2</accession>
    <name>R5VVR2_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine-type D-Ala-D-Ala carboxypeptidase</fullName>
            <ecNumber evidence="4">3.4.16.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">BN566_00460</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the peptidase S11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.4" evidence="4"/>
    <dbReference type="EMBL" id="CBAR010000256">
        <property type="protein sequence ID" value="CCZ84607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00460"/>
    <dbReference type="EnsemblBacteria" id="CCZ84607">
        <property type="protein sequence ID" value="CCZ84607"/>
        <property type="gene ID" value="BN566_00460"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009002">
        <property type="term" value="F:serine-type D-Ala-D-Ala carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015956">
        <property type="entry name" value="Peniciliin-bd_prot_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018044">
        <property type="entry name" value="Peptidase_S11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012907">
        <property type="entry name" value="Peptidase_S11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037167">
        <property type="entry name" value="Peptidase_S11_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001967">
        <property type="entry name" value="Peptidase_S11_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07943">
        <property type="entry name" value="PBP5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00768">
        <property type="entry name" value="Peptidase_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00725">
        <property type="entry name" value="DADACBPTASE1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00936">
        <property type="entry name" value="PBP5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69189">
        <property type="entry name" value="SSF69189"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 18" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="9" id="KW-0133">Cell shape</keyword>
    <keyword evidence="11" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 16" id="KW-0732">Signal</keyword>
    <keyword evidence="15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004395518" description="Serine-type D-Ala-D-Ala carboxypeptidase" evidence="16">
        <location>
            <begin position="34"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="418"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="PBP5_C" evidence="17">
        <location>
            <begin position="308"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="481"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="481"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR618044-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CCZ84607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="542" mass="61046" checksum="4A70D435BDE82F72" modified="2013-07-24" version="1">MRKKWNILQRLLPLLLTFGILLSFCSLPAKAFAFTPPFTIQSESGIVLNLDTNMIIYEKNADKQQMPAHLAQIVTALVVLDACDDLDGTKITMSQNPMSYFYDYTNQEDVRYADIMAGDTFSVREYLYAMLLESSCESAEILADYFGDGNEVQFVKKMNEKAAEIGCVGTNFTNPTGLYDSYQITTARDMLKITQYALQNDTFKEIATTASYVPTSKNANHPASNSLKWMHTNTMMDETDQYYYKGAAGIKTGNLESYGRNIITMATKGDISYLLILMKAPFDDEDGELQYYHMEDATNLLDWAFKDFSYVTLVKNDEELAEVPVESAAGNDYVLVRPESDCITLWCTDVDTSAIQKNINLQENVHAPIEKGQQLGTLELKFSGEVIDTVPLVSVSSVKRSTVKQNLNALREFPHSKMMLYSILVATALTLIYIARCANAAYQCRKKAIEEKSVHLIPKAVRIENNNQKNWKRSSDTVFYHRPEPSEQSQSQSTKSATKKNPQATQKPRTTTATTATTRKRSTSPSQKTMPKRSDTSYTRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVV1</accession>
    <name>R5VVV1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00505</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000266">
        <property type="protein sequence ID" value="CCZ84652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84652">
        <property type="protein sequence ID" value="CCZ84652"/>
        <property type="gene ID" value="BN566_00505"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="270"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="293"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="330"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="368"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="416"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="453"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="483"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="508"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="821" mass="93435" checksum="4841264735016269" modified="2013-07-24" version="1">MGITICSGVGFLAAVGCAGLYDRKSSFLKPFVCGTVFYFFCYILWSMLLFVTNQFTLFRAVLGTAVVLVALLTAAALLLRRKHGTLLRLLHWDESIRTVWIPLVIVLVALPLILTKNELFGMGQDQGVYQVVAINLMNGLTDRQQDFAEYYNLSSADQTEFADAVRNYLMGYDIVPEEYPDTIYDRNISPVSGIYHGIPTYAAMLALFGKLFGISHMIHLETVIWVCMIFLVSFVCRQMQWKRCTEFLACAVCAASPIVIWVAKSSLTELFLAVILLSFVLLITAPNRSHGLSILPVAVFACYHVSIYTMIPLFVVLYGALYLFRRKREYAVLMLATIPLYLASYFAMRHVQPFYTMNNYRDLFVGGITVYNITTVVTVVCIAAFLICGVYCWILFRREKTFDAAAFLEQAQQKPWVKVIFILFMIAPVAYVLLKAFSRGMVGELRYLTLTGYAIQTGFFLLPIVCIIALFRMKFVLEKPETMVLYAMFLYCILVYSAFLRFEIQYYYYYARYLAPFVPIAVLFAAMILDKYNWKLTVPILTAGLLVVAPFDKFLSREKDDTCMEWSILMELGEKIEAGDCVVVDTDLMPQLYLPLRSMTGAAMYPELENAVEQVDNLSEEYDTVYYLTTDTAMLQYSDSYRCVYRNTVHHSEDDLNHNGKLFPMALGFLKYEEPICLYQYTANRMSYRAADCYETEYVGFGALEGDFCWSVAEDASVFCTLHPDNYLMQFEMGCTIPLEQLGKKELTVSVYVNNALAGELVIDESNNGGTLSLPISSLLLLDDTQNQVMLRCELWNASTVTPNDKRQLGFPLKSLRFIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>R5UX29</accession>
    <name>R5UX29_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cobalamin biosynthesis protein CobD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">cobD</name>
        <name evidence="11" type="ORF">BN566_00670</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 10">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cell membrane</location>
            <topology evidence="2 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the CobD/CbiB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000019">
        <property type="protein sequence ID" value="CCZ82868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00670"/>
    <dbReference type="EnsemblBacteria" id="CCZ82868">
        <property type="protein sequence ID" value="CCZ82868"/>
        <property type="gene ID" value="BN566_00670"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015420">
        <property type="term" value="F:ABC-type vitamin B12 transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048472">
        <property type="term" value="F:threonine-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00024">
        <property type="entry name" value="CobD_CbiB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004485">
        <property type="entry name" value="Cobalamin_biosynth_CobD/CbiB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34308">
        <property type="entry name" value="PTHR34308"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03186">
        <property type="entry name" value="CobD_Cbib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 10" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ82868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="174" mass="19145" checksum="E264175FDA4FABAD" modified="2013-07-24" version="1">METVAENTSDGEIAPLFYLFLGGVPLGFFYKAVNTLDSMVGYRNETYEYFGKAAAKLDDFWNWLPARISAWLMMATAVLLRLDGKQALQIYLRDRRKHLSPNSAQTESVAAGAMGIQLGGTHVYFGKVVEKPTIGDAIRHAQPTDILTANKLLFGSSLLFAGLVGGLLLWLSLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5UX72</accession>
    <name>R5UX72_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative stage IV sporulation protein YqfD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00705</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000022">
        <property type="protein sequence ID" value="CCZ82903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00705"/>
    <dbReference type="EnsemblBacteria" id="CCZ82903">
        <property type="protein sequence ID" value="CCZ82903"/>
        <property type="gene ID" value="BN566_00705"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010690">
        <property type="entry name" value="YqfD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06898">
        <property type="entry name" value="YqfD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="400" mass="45311" checksum="9B1BE149E5D88B5A" modified="2013-07-24" version="1">MKRQAAEKRGLWGQVFGVVRIAAQGKKGTAFFDALRAKHIRCFGQQIEKDTVQGVIYAKDCKKMRKLAEQYGMVVTETPCKTLQFFLWRHRLRFGIPIGMILASCFLIYCCNVVMLIEVQGNTTISDEKICAVLEECGVKRGSFMGTVPFRRCEHQLRAAIPELCRVAMRHTGNRLVVEVTETPEEVAPLEKRVPCNIVSAYDAEITSVTVRSGQLLCAVKEPVQKGTLLVSGVQIDEEGNIRLRHAMADIEGIYQIEETFSCAYEQTIRTSSGREQTKTSLDFFTLELPLSKSQKPYAYGQTMTERTPLTIFGKSLPIAICQETNREYTETILQFTPEEAKQDLEQQIQRYERNFLTDVEILEKQVTDTKKETVLCSTVSYTLKGEIGISKEIFAKTEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXA2</accession>
    <name>R5UXA2_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable pectate lyase C</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_00733</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the polysaccharide lyase 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000029">
        <property type="protein sequence ID" value="CCZ82933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00733"/>
    <dbReference type="EnsemblBacteria" id="CCZ82933">
        <property type="protein sequence ID" value="CCZ82933"/>
        <property type="gene ID" value="BN566_00733"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039448">
        <property type="entry name" value="Beta_helix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041253">
        <property type="entry name" value="CBM77"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002105">
        <property type="entry name" value="Dockerin_1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002022">
        <property type="entry name" value="Pec_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13229">
        <property type="entry name" value="Beta_helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18283">
        <property type="entry name" value="CBM77"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00404">
        <property type="entry name" value="Dockerin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00544">
        <property type="entry name" value="Pectate_lyase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00656">
        <property type="entry name" value="Amb_all"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="SSF51126"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="2 3 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004394582" description="Probable pectate lyase C" evidence="5">
        <location>
            <begin position="34"/>
            <end position="1275"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="6">
        <location>
            <begin position="1205"/>
            <end position="1275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="560"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1034"/>
            <end position="1071"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00016512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ82933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="1275" mass="135158" checksum="09DF44A8000E4742" modified="2013-07-24" version="1">MKHTFSFKKGMAAALVAAVTAVASLPSQMLGFAADAVPAADATWEENLSLLASSSVSITKSAGYFEGAYAEWQPVSGADGYNVYCDGVQIDSMLIRQYKDGHLRADALGLKAGSHTLKVVPVKGTSELSGAAEATVSVEANDRSGFGFVKGTSSGAYNEDGTLREDAVVVYVTNENKDTVTASLNAEGKGDVTVTGVQAIINAYKKGKETRPLCLRIIGNITDPTALTKGDLYVDTAKAGMTIEGVGNDAVLNGFGLVMKNCSNIEVRNLGFMNCNSSEGDDCGLQQGNDHIWVHNCDFFYGDAGSDADQVKGDGALDTKKSTYVTHSYNHFWDNGKCNLQGMKSETETNYITYHHNWYDHSDSRHPRIRTCSVHSYNNYFDGNAKYGVGVTMGASAFVENNYFRNCKDPMMSSGQGTDALGEGTFSGEPGGIIKAYNNVIVGASSYIPYAENNTSFDAYEVSSPSETVPSSVTALSGGSTYNNFDTDSSIMYSYTADAPEDVPSIVTAHAGRVDGGDLQWKFDNAVDDTSYAVNQELKNALVAYKSSIVHIGSGFSDSGNNPQVTETKPATTTTSTTTTTITTTKGGVVTTVPAGTTTKPTPVTGALYCAPNAKGVGTMDDPMDIYEAIQKVSAGQTIYLLDGTYELSEMILIDSSNNGAENAYKTISAYPGATVKLDFSGQGAADGSKRGVVLDGDYWKFYGFEITKAADNGMLLAGSHNRIENMVFNDNQDTGLQISRYNTNAATIADWPSYNLILNCTSKNNCDNASMENADGFAAKLTCGEGNVFDGCMAYNNSDDGWDLFAKAATGPIGVVTIQNCVAFRNGFTEFGEGYGNCDGNGFKLGGSGIGSAHVVKNCLSFENLHCGFTDNNNPKLGTLINCTAINNNGEGTGKPNFSCYRCTDPGCDFDNLMSYYDASIFLSDAKLKGGASNDKYVGTYNNGVYYNSGYYLVESDTAITNGAKIGTKFAGPTASDFIALTKAPEQGTDFHKVWRNADGSLNLGGLYETKTDGKYGTMGYHLSNSDTPIVTTTTTSGQNPTTTTTTTTVKPTTTTSGKQSETPTPSGAQIHDFTANGKNSSFYTITGNLATNKGTVSYNGLTLTQSLKMESATSIGFTNTAKGDLTLVFVEPNATVKVDGTKYTANGDGIIQVSVSAGTHTITKADTANLYYMVYADQGGTVVTTTTTATATTTTTTTTINEEGLNYGDVNLDGVVDLADCITVNKYLADVIVLSDIAQKNADVDRDNNVGDKDVSYLMKFVLNSDEVPDLPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXF6</accession>
    <name>R5UXF6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_00005</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000001">
        <property type="protein sequence ID" value="CCZ82750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00005"/>
    <dbReference type="EnsemblBacteria" id="CCZ82750">
        <property type="protein sequence ID" value="CCZ82750"/>
        <property type="gene ID" value="BN566_00005"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="148"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="173"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ82750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="199" mass="21464" checksum="1A8705B2A256AEF8" modified="2013-07-24" version="1">MIHMVADGKFAGYLVLSDTVRTESAGMIAQLKDLNVQPVLLTGDHENAAAAIASQLQMTEVHTNCLPEDKMRWIADEQQKQESVCMMGDGVNDAPALKKANVGIAMGGIGSDIAVDAADIALVDDEVKELPHLLGLSKRMMVTIKCNLTFSMSLNFLAIILAMTGILNPVIGALVHNAGSVLVILNSAWLLKWKRRNEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXG1</accession>
    <name>R5UXG1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative peptidoglycan-binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00560</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000003">
        <property type="protein sequence ID" value="CCZ82755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00560"/>
    <dbReference type="EnsemblBacteria" id="CCZ82755">
        <property type="protein sequence ID" value="CCZ82755"/>
        <property type="gene ID" value="BN566_00560"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.101.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002477">
        <property type="entry name" value="Peptidoglycan-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036366">
        <property type="entry name" value="PGBDSf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013693">
        <property type="entry name" value="SpoIID/LytB_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01471">
        <property type="entry name" value="PG_binding_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08486">
        <property type="entry name" value="SpoIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="SSF47090"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SpoIID" evidence="2">
        <location>
            <begin position="27"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="PG_binding_1" evidence="1">
        <location>
            <begin position="188"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="PG_binding_1" evidence="1">
        <location>
            <begin position="286"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="PG_binding_1" evidence="1">
        <location>
            <begin position="383"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ82755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="466" mass="52895" checksum="B82730F0D13C30A0" modified="2013-07-24" version="1">MPKGEPYIPETITVHLGRPDADAMNVEVPFADYIKNVASSEIYPTWPEAALRANIYAIITFALNRIYTEWYRSRGYPFDITDSTQYDQKYTYGREVFENINRLVDEQLNTYIRRQGTIEPLFAAFCNGTTVTCEGLSQWGTVGLAEQGYSPYDILTYYYGDSIDLVQNVPVQTSMQSYPGFPLALGYSGEDVRLLQIQLNRISRNYPAIPKIGEITGSFGYLTQEAVLKFQEIFNLSPTGTVDEATWYRIDYIYTSVKRLAELDSEGIAQGEFPLQYEEELHIGMQGQNIRALQYYLAMVGAYYANVEPVPITGYFGEQTETSVKSFQRTYGLPETGVVDRATWYDLYRAYMGILDSMPPLAEETEEIVPFPGIVLQEGVTSPYVRILQEYLNYVGQTYPEIGSVRVTGYFGPMTRSAITTFQRLFGLVETGTANVATWNDLTSVYSDLKYGYQKQPYQNPGYIIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXG9</accession>
    <name>R5UXG9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00570</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000005">
        <property type="protein sequence ID" value="CCZ82765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82765">
        <property type="protein sequence ID" value="CCZ82765"/>
        <property type="gene ID" value="BN566_00570"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="72" mass="7463" checksum="D64292D7218C1B3E" modified="2013-07-24" version="1">MPSKYNRTLEPSYPESKNVFAEAVTYGFGFAVVVTTGLAVVATVVVVVTVVVAVVVAFVVAAVVVKSMVVVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXH7</accession>
    <name>R5UXH7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00580</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000007">
        <property type="protein sequence ID" value="CCZ82775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00580"/>
    <dbReference type="EnsemblBacteria" id="CCZ82775">
        <property type="protein sequence ID" value="CCZ82775"/>
        <property type="gene ID" value="BN566_00580"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="112" mass="11551" checksum="C424A1879EA53B7A" modified="2013-07-24" version="1">MADPTFQNGMTPGGTDKNGMAVASLVLGIIAVCCCCVSGLSLVLGILAIVFSILSRKNPAKSGLGTAGLVLGIIASVLAIISIIVAQTDWYQTTVTQQLIDTFGDQYADMLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXP0</accession>
    <name>R5UXP0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00645</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000012">
        <property type="protein sequence ID" value="CCZ82840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82840">
        <property type="protein sequence ID" value="CCZ82840"/>
        <property type="gene ID" value="BN566_00645"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ82840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="174" mass="19290" checksum="AE1CB8B9062AB43A" modified="2013-07-24" version="1">MATTNHSPLDRYMVTEKYIVTRGTALHFPPKIFGKLVTSKDTIYGVVVDMPMSPTLLGTLVMYINGAANLYFNNGAEYIGAAQKYQTVAQPARIAVASAPRLLPEAVKTTATDLPNAQHHYIYLISKNGIYKKDLRLANLPNESKETQSFFYLYQQVMGALQNAQVKDRSNAGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXP2</accession>
    <name>R5UXP2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Surface antigen BspA-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00851</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000047">
        <property type="protein sequence ID" value="CCZ83063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00851"/>
    <dbReference type="EnsemblBacteria" id="CCZ83063">
        <property type="protein sequence ID" value="CCZ83063"/>
        <property type="gene ID" value="BN566_00851"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026906">
        <property type="entry name" value="LRR_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13306">
        <property type="entry name" value="LRR_5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="178"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="457" mass="50305" checksum="3A2EB164BBAD7A5F" modified="2013-07-24" version="1">MAFSYGFFNAKNLDRVYTAEHFTSYLSSIICDGIQDTYGECFSITPAGGFQLRIGSGKAWIQGHYFQNDNGYILDLSQYADSSLPRYVTVGISCDTQESVRSVQIEVLAGTPAVAPFIPSFSNNGTKTTLTLCQVRVNGGSSGITASNITDCREDEELCGYCRCILGKCRVTEMLTKMEQTNAYLKQLQKRLDDMSNQVADLQTKVDDLTGGEVSETGICGENVYYVLYANGKLLLRGTGATYDYKAESTVFGSNLNIKNIVVSNGITSLGDNLFYHCENAVAVDLPSTLTSIGDNTFSQFEAERTINGLTAVTIPQTVTAIGQHAFEQNAITEIIIPASVKTWGDYAFSGCRKLTTARIECSLIGSFAFSWCDLLDGLTISTNCKNFGSNLFTYCEKLTAITYEGTKEQWNAITKPTNWMASDAKTNYHNGYLQRINCIDGAFVWNSKNMDWEEET</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXQ3</accession>
    <name>R5UXQ3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cytidylate kinase</fullName>
            <shortName evidence="8">CK</shortName>
            <ecNumber evidence="8">2.7.4.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Cytidine monophosphate kinase</fullName>
            <shortName evidence="8">CMP kinase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">cmk</name>
        <name evidence="10" type="ORF">BN566_00871</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP + CMP = ADP + CDP</text>
            <dbReference type="Rhea" id="RHEA:11600"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 8">
            <text>ATP + dCMP = ADP + dCDP</text>
            <dbReference type="Rhea" id="RHEA:25094"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57566"/>
            <dbReference type="ChEBI" id="CHEBI:58593"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.25"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the cytidylate kinase family. Type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.25" evidence="8"/>
    <dbReference type="EMBL" id="CBAR010000051">
        <property type="protein sequence ID" value="CCZ83083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00871"/>
    <dbReference type="EnsemblBacteria" id="CCZ83083">
        <property type="protein sequence ID" value="CCZ83083"/>
        <property type="gene ID" value="BN566_00871"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036430">
        <property type="term" value="F:CMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036431">
        <property type="term" value="F:dCMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006220">
        <property type="term" value="P:pyrimidine nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02020">
        <property type="entry name" value="CMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00238">
        <property type="entry name" value="Cytidyl_kinase_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003136">
        <property type="entry name" value="Cytidylate_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011994">
        <property type="entry name" value="Cytidylate_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02224">
        <property type="entry name" value="Cytidylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00017">
        <property type="entry name" value="cmk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidylate_kin" evidence="9">
        <location>
            <begin position="6"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="10"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ83083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="223" mass="25151" checksum="DBEBE1AE88EDDF91" modified="2013-07-24" version="1">MKTMNVAIDGPAGAGKSSIARELAAELGFIYVDTGALYRAIALYTQQHNLCNKQDIIRQLPKIDLKIKFIGGTQCILLNGEDVTDDIRTPEISMLASKVSAIPEVRDFLFDLQQKIAKEHHVIMDGRDIGTVVLPDADLKIFLTASPEERASRRYLELQEKPDAPTYEQILQDIKKRDYQDTHREIAPLRQAEDAVLIDTTNMGFHEVCDALLELMEERLDLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXX3</accession>
    <name>R5UXX3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CHAP domain./Lectin C-type domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00720</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000029">
        <property type="protein sequence ID" value="CCZ82920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00720"/>
    <dbReference type="EnsemblBacteria" id="CCZ82920">
        <property type="protein sequence ID" value="CCZ82920"/>
        <property type="gene ID" value="BN566_00720"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="SSF56436"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="89"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ82920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="241" mass="27311" checksum="BAE3E8E5F9FA7E32" modified="2013-07-24" version="1">MNLFKKSDEPVFSKNLEQIDSIQSSQKPSSKKPLLIAVVIIVICLLLVAGSLTALFWLHHTNTTDDAVREPSQTTVRSDIPDSALVNPQNGHSYFAYTDSVSWIDAKTACESLGGHLATISDAQEQTFVEQLSQTCSERTNFWLGGYYDRNRDIWKWVDGTPFTYTNWDSWDNGETEFSQPDNHTGNEWYIRFGKSDQTYETWVEHAGCWNDIANRAGDADDDVPLDSFGFLCEWDSTNNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5UY38</accession>
    <name>R5UY38_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">FAD dependent oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00775</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000034">
        <property type="protein sequence ID" value="CCZ82975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00775"/>
    <dbReference type="EnsemblBacteria" id="CCZ82975">
        <property type="protein sequence ID" value="CCZ82975"/>
        <property type="gene ID" value="BN566_00775"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="domain" description="DAO" evidence="2">
        <location>
            <begin position="6"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ82975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="375" mass="40466" checksum="C41E9140335A649C" modified="2013-07-24" version="1">MTKTADVIVIGSGVIGCAAAYYMAKKGMSVLVLDQDESVGNGGSSRNGGGVRQSGRDPRELPLAIYGVENVWPTLSDELGVDVEYHKEGNLRLGKTETHKQILTGLTEKAVACGLNVRMIDAEEVRKINPYLSEEVTCASWCPTDGHANPMTTTLGFYKNARALGVVFHMGEKVVKLEKVHGKLRRVCTKTTVYEADQVLVAAGYASRFLTQTVGIDVPMRKELIEALVTEAEPKMFPQMLGTADADFYGHQTNHGSFVFGGESGMESTVLDNGTNRTSSLTAPCICRGIMKYVPKLADAKIVRTWAGYEDLSIDGIPVISKVEEVPGLLLACGFTGHGFGISPVVGQLLAQLAAGETPMLSLQEFRYDRFHAAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>R5UY85</accession>
    <name>R5UY85_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sulfate transport system permease protein CysT</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN566_01069</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Part of the ABC transporter complex (TC 3.A.1.6.1) involved in sulfate/thiosulfate import.</text>
    </comment>
    <comment type="function">
        <text evidence="8">Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">The complex is composed of two ATP-binding proteins (CysA), two transmembrane proteins (CysT and CysW) and a solute-binding protein (CysP).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01069"/>
    <dbReference type="EnsemblBacteria" id="CCZ83288">
        <property type="protein sequence ID" value="CCZ83288"/>
        <property type="gene ID" value="BN566_01069"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015419">
        <property type="term" value="F:ABC-type sulfate transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011865">
        <property type="entry name" value="CysT_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005667">
        <property type="entry name" value="Sulph_transpt2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30406">
        <property type="entry name" value="PTHR30406"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30406:SF8">
        <property type="entry name" value="PTHR30406:SF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00969">
        <property type="entry name" value="3a0106s02"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02139">
        <property type="entry name" value="permease_CysT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0764">Sulfate transport</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="59"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="136"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="241"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="59"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU366001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ83288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="277" mass="30018" checksum="C90BFD7ABA61EABF" modified="2013-07-24" version="1">MKQKSKRVIPGFGISMGVTLTILSIVVLIPLASLVVYTAQLSPSEIIETLTRPRVLASFRVSLLTAFIASAINAVMGVILAWVLVRYKFPLKRILDGMIELPFALPTAVAGIALTSLTADTGLIGKFFARFGIHIAYTRVGITFALIFIGIPFVVRTVQPVLEKLDPSYEEAAGVLGASRTKIFWSVVFPEIRPAVITGFGLAFGRCLGEYGSVVFIAGNQPFQTEITPLIIMSELQEYDYASATAIALVMLIVAFLILFLSNWVQARSSKKLKGGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYJ1</accession>
    <name>R5UYJ1_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ion-translocating oxidoreductase complex subunit E</fullName>
            <ecNumber evidence="7">7.-.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Rnf electron transport complex subunit E</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rnfE</name>
        <name evidence="8" type="ORF">BN566_00883</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Part of a membrane-bound complex that couples electron transfer with translocation of ions across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">The complex is composed of six subunits: RnfA, RnfB, RnfC, RnfD, RnfE and RnfG.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the NqrDE/RnfAE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.-.-.-" evidence="7"/>
    <dbReference type="EMBL" id="CBAR010000054">
        <property type="protein sequence ID" value="CCZ83095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00883"/>
    <dbReference type="EnsemblBacteria" id="CCZ83095">
        <property type="protein sequence ID" value="CCZ83095"/>
        <property type="gene ID" value="BN566_00883"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022900">
        <property type="term" value="P:electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00478">
        <property type="entry name" value="RsxE_RnfE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003667">
        <property type="entry name" value="NqrDE/RnfAE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010968">
        <property type="entry name" value="RnfE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02508">
        <property type="entry name" value="Rnf-Nqr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006102">
        <property type="entry name" value="NQR_DE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01948">
        <property type="entry name" value="rnfE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="96"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="167"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="222" mass="23689" checksum="88D9556C653726C1" modified="2013-07-24" version="1">MNYGKEFTKGLIRENPTLVSLLGMCPTLAVTTGAFNGIGMGLSTAFVLIFSNLVISLLRKVIPEQVKLPCYIVIIATFVTLIQFLLHGFVPALYTNLGAFLELITVNCIILGRAEMFASKNTPLPSILDGAGMGLGFTLALFLMGSIREIFGSGSWFGLSIPFMQGHTMSLFVMPAGGFFVLGIIIAVVCRLSKKKPPKKIGCSACPNAAACRAANCEEVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYL6</accession>
    <name>R5UYL6_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8 9">Translation initiation factor IF-2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">infB</name>
        <name evidence="12" type="ORF">BN566_01184</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 8 9">One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 9">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000097">
        <property type="protein sequence ID" value="CCZ83408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01184"/>
    <dbReference type="EnsemblBacteria" id="CCZ83408">
        <property type="protein sequence ID" value="CCZ83408"/>
        <property type="gene ID" value="BN566_01184"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03702">
        <property type="entry name" value="IF2_mtIF2_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00100_B">
        <property type="entry name" value="IF_2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044145">
        <property type="entry name" value="IF2_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006847">
        <property type="entry name" value="IF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000178">
        <property type="entry name" value="TF_IF2_bacterial-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015760">
        <property type="entry name" value="TIF_IF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023115">
        <property type="entry name" value="TIF_IF2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036925">
        <property type="entry name" value="TIF_IF2_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381">
        <property type="entry name" value="PTHR43381"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11987">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04760">
        <property type="entry name" value="IF2_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52156">
        <property type="entry name" value="SSF52156"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00487">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 8 9" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="11">
        <location>
            <begin position="373"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="76"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="239"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="G-domain" evidence="8">
        <location>
            <begin position="376"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="80"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="107"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="190"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="382"/>
            <end position="389"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="428"/>
            <end position="432"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="482"/>
            <end position="485"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CCZ83408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="871" mass="96139" checksum="A546F8C28BA648B3" modified="2013-07-24" version="1">MTTKKQKLSDVAKTLQMTPNDLIALFSELQNGTEKKSSSTLTEAEVNLVLEYVTQHQQVTDGFAAYFATKNSTREEVAQQSMKKGNEKKAAHQQQNQGKKQPQDRRRPQQQNAAGNNHANNNNGNGNGNPNNSNSAAKKPQQAASNQKNQPQAAAQPKKQPAAAKPASQPNNNNNNNHPEHHKKKQKQQPQAQKRGERLHVEVELSSDTATTTEKRRTVDTRGSYVDLDKYNQRYEQIAPAGKYSKDNYSTKKQKINQKSAQRNKQKYSNKRETEQDRLRRLELEKARKQQLKVRIPESIVVSELASRLKVTATEVIKKLMGLGVMASINEEIDFDTACLVAEELGAKVEKEVIVTIEERLIEEEDESDNTEERSPVVVVMGHVDHGKTSILDYIRHANIAAGEAGGITQHIGAYQVTCNGKEITFLDTPGHEAFTAMRARGANITDIAILVVAADDGIMPQTVESINHAKAAGVSLIVAINKMDKEGANPDRVKEELTKYDLVCEDWGGDVMCVPVSAKTGEGIDELLEDVLLIAEMQELKANPNRRAKGAVIEARLDKGRGPVATLLVQNGTLHTGDVILAGTSVGKVRVMTNDKGTVVHEAGPSVPVEITGLAEVPAAGDVFDVVEDERLAKTLVEQRKHEAKQAKFSEYQKVTLDNLFSQIAEGEMKELAIIVKADVQGSVEAVKQSLEKIQNDEVRVRVIHGGVGAINESDVMLADASNAIIIGFNVRPDPLAEETAARDHVEIRTYRVIYDAIEDVETAMKGMLAPKFREVVMGRIEVRQVYKISSVGAVAGAYVLSGKVTRQCQIRVVRDGIVIAEDKMSSLKRFKDDVKEVTEGYECGITLEKFRDFKEGDIFEAFIMEEYQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYP9</accession>
    <name>R5UYP9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NAD kinase</fullName>
            <ecNumber evidence="5">2.7.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">ATP-dependent NAD kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">nadK</name>
        <name evidence="7" type="ORF">BN566_01212</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + NAD(+) = ADP + H(+) + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:18629"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the NAD kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.23" evidence="5"/>
    <dbReference type="EMBL" id="CBAR010000105">
        <property type="protein sequence ID" value="CCZ83438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01212"/>
    <dbReference type="EnsemblBacteria" id="CCZ83438">
        <property type="protein sequence ID" value="CCZ83438"/>
        <property type="gene ID" value="BN566_01212"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019674">
        <property type="term" value="P:NAD metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006741">
        <property type="term" value="P:NADP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00361">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017437">
        <property type="entry name" value="ATP-NAD_kinase_PpnK-typ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002504">
        <property type="entry name" value="NADK"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01513">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="SSF111331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 5" id="KW-0520">NAD</keyword>
    <keyword evidence="3 5" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="285"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="285"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="69"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="69"/>
            <end position="70"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="143"/>
            <end position="144"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="183"/>
            <end position="188"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ83438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="308" mass="34209" checksum="166567355C8DA5A4" modified="2013-07-24" version="1">MKAVVFPNLKKDPALSCAREVCDVLQACNITPIADPELRALFFDKSFVQYQPLLEAVQEAEFAIAIGGDGTILRCASHLIHTNTKLLGINTGRLGFMAALEPSQIQEIARLRTGDYRVSSRMLLCGDLLDLHGHVVQHCTALNDITIARQYAHVVDFSVYRNDVLLGEYRADGVLFSTPTGSTAYALSAGGPIIEPEFSCIEMTLICPHSLSARPILFSPESRLCVSLQEQDVRDVYISADGAPPICLDAYHRMEIYRSPHEISLIDLSGNTFFDSLNRKMMQSLKGESEKEKVTKWEKKSDTPQSWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYZ0</accession>
    <name>R5UYZ0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Carbohydrate binding module (Family 6)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01272</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000119">
        <property type="protein sequence ID" value="CCZ83503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01272"/>
    <dbReference type="EnsemblBacteria" id="CCZ83503">
        <property type="protein sequence ID" value="CCZ83503"/>
        <property type="gene ID" value="BN566_01272"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004394499" evidence="1">
        <location>
            <begin position="36"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="262" mass="29816" checksum="B6AF7E3A367CC720" modified="2013-07-24" version="1">MKAPQKILAVGVASLLCFGSVCTELSLPNTTATLAASTASTLADFPSSYQSAADWIWTNRIEREQSTVRRNTLFDQIIAGNGELHYVVRWQSYKTVTLEQRRQFQQLVEDSVNAWTDWLRGYDDWPYDHVTVKIVGWAVLDRNCLLDLQPDEVVYDSLISDYDSTYDTSNGYETIPNKLPSAPSERSRMNHFMDTNYVYPVERFDMYLWATQGFPSIGGCGGDWGQRXXXGDWGQRLSDDAYLNMLNGTNLHVLEHELGHGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZ10</accession>
    <name>R5UZ10_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_01302</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000121">
        <property type="protein sequence ID" value="CCZ83533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01302"/>
    <dbReference type="EnsemblBacteria" id="CCZ83533">
        <property type="protein sequence ID" value="CCZ83533"/>
        <property type="gene ID" value="BN566_01302"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="5"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="41"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="234" mass="25683" checksum="4DF13B19690F2485" modified="2013-07-24" version="1">METLLQVQEICKYYRMGEVQITAVDHVSFSVKKGEFVVIVGASGAGKTTVLNLLGGMDTCDSGSILVDGTDITKLRKRKLAAYRRFDIGFVFQFYNLIPNLTAVENVELAGHISRDAMPAAEALQYVGLGDRLRHFPAQLSGGEQQRVSIARALAKRPKLLLCDEPTGALDYETGKSVLKLLQDTCRDTQMTVLLITHNQAITPMADRVITMKNGKIAAMQQNAEPVPVSEIAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZ87</accession>
    <name>R5UZ87_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01126</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000092">
        <property type="protein sequence ID" value="CCZ83350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83350">
        <property type="protein sequence ID" value="CCZ83350"/>
        <property type="gene ID" value="BN566_01126"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="72" mass="8412" checksum="2A2BE1B1FFEBC0CA" modified="2013-07-24" version="1">MEMDYGARLRELRQAKKFSIYRLHEITGLSQGHISGLEKAKTSLRLKPCSDFFLLWAFRSLSFSMRTMKSPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZB9</accession>
    <name>R5UZB9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01156</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000093">
        <property type="protein sequence ID" value="CCZ83380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83380">
        <property type="protein sequence ID" value="CCZ83380"/>
        <property type="gene ID" value="BN566_01156"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="214" mass="24930" checksum="A177E1773002E2B2" modified="2013-07-24" version="1">MLLAADFLLYIVRWCSIIASHSTKSNVKIVIAGIFFIFLFLYLFSKILFWTETGSFHRTITKTDAVEFVIECDPFDVLAVAKELEPYAKKYTDVEVELKYTEYSFKGNEITAMYGFKGTKYRKGRGETIQFYLDIDKHTLYEISYFAAGGKEFSSFPSQYFNANINIMDEYEKIKNSNPDFDSIEEIQFILSNNQCESLYKQKRTTRSLLLCLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZX4</accession>
    <name>R5UZX4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">3-isopropylmalate dehydrogenase</fullName>
            <ecNumber evidence="7">1.1.1.85</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">3-IPM-DH</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Beta-IPM dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">BN566_01289</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R,3S)-3-isopropylmalate + NAD(+) = 4-methyl-2-oxopentanoate + CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:32271"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17865"/>
            <dbReference type="ChEBI" id="CHEBI:35121"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.85"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.85" evidence="7"/>
    <dbReference type="EMBL" id="CBAR010000121">
        <property type="protein sequence ID" value="CCZ83520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01289"/>
    <dbReference type="EnsemblBacteria" id="CCZ83520">
        <property type="protein sequence ID" value="CCZ83520"/>
        <property type="gene ID" value="BN566_01289"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00072"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003862">
        <property type="term" value="F:3-isopropylmalate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004429">
        <property type="entry name" value="Isopropylmalate_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42979">
        <property type="entry name" value="PTHR42979"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="15" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0432">Leucine biosynthesis</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0520">NAD</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Iso_dh" evidence="18">
        <location>
            <begin position="1"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00019276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00030010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00033138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CCZ83520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="225" mass="24779" checksum="84BC18872C1990E1" modified="2013-07-24" version="1">MMVRELTGGIYFGERGRRTGKYGEEAYDTECYSRMEIERIAKVAFETAQKRNKNVISIDKANVLESSRLWREVVHEVAKDYPDVTCTDMLVDNAAMQLVKNPRQFDVVVTSNMFGDILSDEASQITGSIGMLPSASLGSTKRGLYEPIHGSAPDIAGQNKANPIATILSAAMMLRYSFGLMDEAKAIEDAVDKYLEAGYRTADLAKEGVTPLSCTECTAKIIELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5V047</accession>
    <name>R5V047_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein conserved in bacteria</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01692</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000177">
        <property type="protein sequence ID" value="CCZ83963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01692"/>
    <dbReference type="EnsemblBacteria" id="CCZ83963">
        <property type="protein sequence ID" value="CCZ83963"/>
        <property type="gene ID" value="BN566_01692"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045735">
        <property type="entry name" value="Spore_III_AA_AAA+_ATPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19568">
        <property type="entry name" value="Spore_III_AA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="3">
        <location>
            <begin position="30"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="181" mass="19659" checksum="D2AA0065FD1C53E1" modified="2013-07-24" version="1">MRNISGLNIRLARQVKDCAKELAAKLCSQRLTSILLAGTVGSGKTTMLRDLCRILGQTYRLCIIDSRGELAAVQNSVPQYEIGLHTDVYDGFPRAEGAVMALRVMAPDGLVCDEISTAEDAAALMQVYGCGVQILATAHAANEQDLYRRTALRTLLEAGVFEYLVLLRQGSVQKIQRVAAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5V094</accession>
    <name>R5V094_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01416</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000148">
        <property type="protein sequence ID" value="CCZ83680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01416"/>
    <dbReference type="EnsemblBacteria" id="CCZ83680">
        <property type="protein sequence ID" value="CCZ83680"/>
        <property type="gene ID" value="BN566_01416"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="129" mass="14709" checksum="35CAA43776E33EC9" modified="2013-07-24" version="1">MGVSKTITIDGVDVQFKASAAIPRLYRLQFRRDLFHDFADLQKSVDDEKEKDSEASGLNPEILETFENVAYMMAKHADPKGVPGTAEEWLEQFSMFSIYEILPELLELWNANLQTQVQSKKNIARLTAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0A5</accession>
    <name>R5V0A5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01431</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000153">
        <property type="protein sequence ID" value="CCZ83695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01431"/>
    <dbReference type="EnsemblBacteria" id="CCZ83695">
        <property type="protein sequence ID" value="CCZ83695"/>
        <property type="gene ID" value="BN566_01431"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="9"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="153" mass="17892" checksum="265B45EAD75A5BE0" modified="2013-07-24" version="1">MQALPETKRLFLREWTMQDLDAWAAILSDAEVMRYYPKPFDRQKVQDWIAWNLENYRTDGFGLWAVHLRATGELLGDCGITMQQIHRQHLPEIGFHMKKSVWGQGYATEAAKACLAWGMQQTDFPAFYCYQKSTNLPSQERNFSQEHKTPCDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0D2</accession>
    <name>R5V0D2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phosphoglycerate mutase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01754</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000180">
        <property type="protein sequence ID" value="CCZ84028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01754"/>
    <dbReference type="EnsemblBacteria" id="CCZ84028">
        <property type="protein sequence ID" value="CCZ84028"/>
        <property type="gene ID" value="BN566_01754"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="SSF53254"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="193" mass="22314" checksum="51AC4738DCDCE5EB" modified="2013-07-24" version="1">MQILFFRHSMTAGNFERRYLGCKTDEPLCPEGIAFATQQAKQISFPLPEQVFVSPMRRCRETASILFPNLKQQIHPDLRECDFGMFEGKNYRELSNCSEYQAWIDSGGTLPFPDGESREQFLTRSCAAVSEIVQTCKSDRIAIVAHGGTMMAVFSACEMHHKSYFDWKIPHCEPFLCEVFRKNPLQLQWKERN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0E5</accession>
    <name>R5V0E5_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Diaminopimelate epimerase</fullName>
            <shortName evidence="8">DAP epimerase</shortName>
            <ecNumber evidence="4 8">5.1.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">PLP-independent amino acid racemase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">dapF</name>
        <name evidence="10" type="ORF">BN566_01774</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>(2S,6S)-2,6-diaminoheptanedioate = meso-2,6-diaminoheptanedioate</text>
            <dbReference type="Rhea" id="RHEA:15393"/>
            <dbReference type="ChEBI" id="CHEBI:57609"/>
            <dbReference type="ChEBI" id="CHEBI:57791"/>
            <dbReference type="EC" id="5.1.1.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; DL-2,6-diaminopimelate from LL-2,6-diaminopimelate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the diaminopimelate epimerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.1.1.7" evidence="4 8"/>
    <dbReference type="EMBL" id="CBAR010000182">
        <property type="protein sequence ID" value="CCZ84048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01774"/>
    <dbReference type="EnsemblBacteria" id="CCZ84048">
        <property type="protein sequence ID" value="CCZ84048"/>
        <property type="gene ID" value="BN566_01774"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00025"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008837">
        <property type="term" value="F:diaminopimelate epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00197">
        <property type="entry name" value="DAP_epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018510">
        <property type="entry name" value="DAP_epimerase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001653">
        <property type="entry name" value="DAP_epimerase_DapF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31689">
        <property type="entry name" value="PTHR31689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01678">
        <property type="entry name" value="DAP_epimerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00652">
        <property type="entry name" value="DapF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01326">
        <property type="entry name" value="DAP_EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6 8" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="73"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="214"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="224"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="72"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="72"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="223"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Could be important to modulate the pK values of the two catalytic cysteine residues" evidence="8">
        <location>
            <position position="165"/>
        </location>
    </feature>
    <feature type="site" description="Could be important to modulate the pK values of the two catalytic cysteine residues" evidence="8">
        <location>
            <position position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ84048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="283" mass="31313" checksum="7651DEE92CC01E1B" modified="2013-07-24" version="1">MLTFSKMHGIGNDYIYINCFQETVTDPEKLSIFMSNVRFGVGSDGLVLILPSEVADFRMRIFNADGSEAMMCGNATRCVGKYVYEHGMTDKTEVSLETNSGIKYLTLYVNEATNKVDAVKVDMGKAILKPAEIPVADDGDRFIAKPVVVDGVSYDMTCVSMGNPHAVVFLPEIDSLDLEKIGPSFEHHPLFPNRVNTEFIRVIDDHTLQMRVWERGSGETFACGTGACAAAVASVLNGYCKREQEILIHLRGGDLRIIYHNDESVTMIGPATFIFEGKMEYPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0H2</accession>
    <name>R5V0H2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01809</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000186">
        <property type="protein sequence ID" value="CCZ84083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84083">
        <property type="protein sequence ID" value="CCZ84083"/>
        <property type="gene ID" value="BN566_01809"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="110" mass="12392" checksum="373614118D27253F" modified="2013-07-24" version="1">MLVFIGLILVYGLLFAILAVSKAILDKDKKPPTSSKSSHTQSSSRKPAYPVKTVPKQRRSTVDEDAWILLQSRKKSAPKPARRSSKPAKPRPKFHDPDCECEDCEEREDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0J7</accession>
    <name>R5V0J7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01839</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000187">
        <property type="protein sequence ID" value="CCZ84113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01839"/>
    <dbReference type="EnsemblBacteria" id="CCZ84113">
        <property type="protein sequence ID" value="CCZ84113"/>
        <property type="gene ID" value="BN566_01839"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="5"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="35"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="234" mass="24936" checksum="6E05C6FAC1FD053A" modified="2013-07-24" version="1">MMKRTIIRIDEAACIGCGACAEACHEGAIGMVNGKAKLLREDYCDGLGDCLPACPTNAITFEEREAPAYNEQAVLAAKAAKAAAKPLPCGCPGTQAKQLKRTAAPAVCPQGETESCLAQWPVQIQLISPVAPYLQGADLLIAADCTAYAYHDFHEQFMRNRITLIGCPKLDTVDYAEKLTEIFATCSIRSITVVRMEVPCCGGLVQAVQRALTASGAEIPCEVVILSTDGRRKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0N7</accession>
    <name>R5V0N7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phosphoenolpyruvate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01883</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000193">
        <property type="protein sequence ID" value="CCZ84158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84158">
        <property type="protein sequence ID" value="CCZ84158"/>
        <property type="gene ID" value="BN566_01883"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="710" mass="82952" checksum="7CB8E796C26FB91A" modified="2013-07-24" version="1">MIIDFVNYTLYHPGAEASILFHLHEYGLRVPRFFCIPEQYEPEELDAYFAMHFQDVLQYHVWMTATIVVSKNKTDRTEWMPNCKADLPVYTNVKKYALYRNIERILESRESYRQKIAAAESVSPEQVQFHIIIQEARTFTEFAVLNIIQQDGILNETQIQIGCGWNSELVWNDTPTITMCYHNTDQLLYYGLPAGTTPPEDDFLRKLEKAAQILFTKFDIQMQVSIFVDPESHHLFALTVRQTTLPSEESILFDSRSILSAFYPTTCTPLMGSYMQNMYNHVIHSFFKTTLHLFDRPYSVSDDMDDIVIYINGRLYFCVEHWNKLLYYFPTFRKKQSFKNVYALLHDRDGIRSWMVHRYIDYIGRRLMRSDFKVVQEKLQLLENRVQVYQSYDLSRMSLSELNQYYKRFQNTNIACWHYTLLSVLNSRISIQHLKKSLRKTHPNLPSGIQYQYAAGMLPMPDWAGKKLRKKIRCTQKSETYRKQFLKLQDQLVKLLADLFLAAGNRLYEAGSISDVDDVHYLVLKEVLASMENPSIQWKDNRSAWRREAYRWYLNLPNYTEMVLQKKCIGQQEEPLTHMVFPSGSGKTQGRPCMPGLSKGDILIYDGKQPLPEEQAAGKIIITAHLSKFLLSLPLKGLILEDELTCEQVSTQANPRVCFPVLSGVRGACSLFQHTQQIYLNGWTGEITASREQTSEKTKTLWSEYRSRHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0P1</accession>
    <name>R5V0P1_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dockerin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01888</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000193">
        <property type="protein sequence ID" value="CCZ84163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01888"/>
    <dbReference type="EnsemblBacteria" id="CCZ84163">
        <property type="protein sequence ID" value="CCZ84163"/>
        <property type="gene ID" value="BN566_01888"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="3">
        <location>
            <begin position="940"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="258"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="865"/>
            <end position="921"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="1012" mass="109739" checksum="ECCEA3AE84FDF6DF" modified="2013-07-24" version="1">MCCNARENDVSVYRPQSVCGKPYTIYVYEQKGKAMKKITKIISFCAAVTMAATAVPASSLLMAQAADVAKITVGKDAAPIVVNPGDKEVEIPLYMETTRDVEGISFGFVTTGEGKATKELLNANSDTYIVAEPADAFVSLGKWEKNPAGLSWGVPSSGKAVVKASDVSPDEPFISLYYNIPDEKTVTTIAQNNGLKKVNDGDKAYYDFPLDIDRESMNSKDGKLFTWIGPNNTHYEDEAEVSNTVIRVYVTPDAPDVTTTTATTTTTAQTTTTTVTTTTDDTKDDTLPQLNVNGGKDLFVEPGQQEAEIPLYIKNPIDTTALSFGFKLDAATVALLDSSNYVTHEASDPFVSLGKWEKNPKGLSWGVASSGKTVVKASDISDDEAIVSLYYNIPNKATVQKIADDNSLELKTGTYNGKTVKYYEFPLQFDRERLNSKLGHIVEWSSATDSHVKANYVGNNLCVVMETVSSDNPEINVNKDEIVIVDAGQKEAEIPLYMKNAEDTVGITVAFKTDAEGKPTQELLNKNSDTYVIAEPADVFVSLGKWENNPKGLSWGVPSNGKTTALASDISEEEAFFSLYYNIPSKEDVESIAEANGLEEKKDGEGSTYYDFPLVFEREKLNNKDGKLLDWIGPNDVSIRERAIYQDNILRVYTNRKNITQVDTRVEVSGEETTDASGNTVDSHKIFVEPGQQEAEVPLKVYDAVDATAVSFAFQTDGEGAATQKLLNSEGAYQDGFYAFADALLNLGKPLDNNAELIWGISGDGVKIATADSMPDGSDILSIYYNIPNVTADDLAAMGGKLVKADDPDNIKGKDYYEFPLNFDKEKKNSRGGFALEWITKDEEQVDVTYVDGSICVLVPEETTTTTTTTTTTTTTTTTTTTTTTTAKPTTTTTKATTTTTTTTTAKPTTTTTTTTTTAKPTTTTTKATTTATTTTKKPLVPLYGDVNVNGQVTIVDVVLLNKAIAGKVTLDEQAFLNADCGNVDQVLDEHDLNALMQYLVGYVQQLPGEMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0Q5</accession>
    <name>R5V0Q5_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">UDP-N-acetylmuramate--L-alanine ligase</fullName>
            <ecNumber evidence="4 12">6.3.2.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">UDP-N-acetylmuramoyl-L-alanine synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">murC</name>
        <name evidence="16" type="ORF">BN566_01585</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Cell wall formation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>ATP + L-alanine + UDP-N-acetyl-alpha-D-muramate = ADP + H(+) + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine</text>
            <dbReference type="Rhea" id="RHEA:23372"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:70757"/>
            <dbReference type="ChEBI" id="CHEBI:83898"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the MurCDEF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.8" evidence="4 12"/>
    <dbReference type="EMBL" id="CBAR010000167">
        <property type="protein sequence ID" value="CCZ83850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01585"/>
    <dbReference type="EnsemblBacteria" id="CCZ83850">
        <property type="protein sequence ID" value="CCZ83850"/>
        <property type="gene ID" value="BN566_01585"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008763">
        <property type="term" value="F:UDP-N-acetylmuramate-L-alanine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00046">
        <property type="entry name" value="MurC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004101">
        <property type="entry name" value="Mur_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000713">
        <property type="entry name" value="Mur_ligase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005758">
        <property type="entry name" value="UDP-N-AcMur_Ala_ligase_MurC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01225">
        <property type="entry name" value="Mur_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02875">
        <property type="entry name" value="Mur_ligase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="SSF53244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01082">
        <property type="entry name" value="murC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6 12" id="KW-0132">Cell division</keyword>
    <keyword evidence="8 12" id="KW-0133">Cell shape</keyword>
    <keyword evidence="11 12" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 16" id="KW-0436">Ligase</keyword>
    <keyword evidence="12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 12" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mur_ligase" evidence="13">
        <location>
            <begin position="11"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_M" evidence="15">
        <location>
            <begin position="118"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_C" evidence="14">
        <location>
            <begin position="319"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="120"/>
            <end position="126"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CCZ83850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="481" mass="52650" checksum="FE15F171591C2C26" modified="2013-07-24" version="1">MNKEILNCKKHIHFIGIGGSGMYPLVQILHTKGYYITGSDNNESETLQAVRDMGIPVMMGQRAENIEGADLIVYTAAIMADNPELIAAKEAAKHGVDLMERSELLGLVTSWYDNAICIAGTHGKTTTTSMTTQILFDAGADPSAVIGGKLACIHGSGRCGNSDIMTCEACEFVDTFLKLYPDIAVLLNIDADHLDYFGTVENIIRSFHKFAENTSKVVIYNGDDEKTCKAVEGITGKELITFGEKEGNDYVATNIIHVSGLETHFDVLYHGELLGTVVLHIAGVHNVLNALAAIAAVRYVGISFEDAVKGLGNFHGAGRRFDKKGEACGVTVVDDYGHHPTELTVTLQAAMEMPFHAVWAVFQPFTYSRTAVLLDDFAKALSIPTHAVLTDIMGSREKNTYNIFTRQLGEKIPGAVWFPQDETDPESNDERKYKNFADVCQYVCDHVEPGDLVITMGCGDINKVNKMILEELQRREQAASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1G1</accession>
    <name>R5V1G1_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Endonuclease MutS2</fullName>
            <ecNumber evidence="7">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">mutS2</name>
        <name evidence="11" type="ORF">BN566_01816</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Endonuclease that is involved in the suppression of homologous recombination and may therefore have a key role in the control of bacterial genetic diversity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DNA mismatch repair MutS family. MutS2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="7"/>
    <dbReference type="EMBL" id="CBAR010000186">
        <property type="protein sequence ID" value="CCZ84090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01816"/>
    <dbReference type="EnsemblBacteria" id="CCZ84090">
        <property type="protein sequence ID" value="CCZ84090"/>
        <property type="gene ID" value="BN566_01816"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045910">
        <property type="term" value="P:negative regulation of DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03280">
        <property type="entry name" value="ABC_MutS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00092">
        <property type="entry name" value="MutS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005747">
        <property type="entry name" value="MutS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS_family"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002625">
        <property type="entry name" value="Smr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036063">
        <property type="entry name" value="Smr_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="PTHR11361"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF14">
        <property type="entry name" value="PTHR11361:SF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01713">
        <property type="entry name" value="Smr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005814">
        <property type="entry name" value="MutS_YshD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00463">
        <property type="entry name" value="SMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160443">
        <property type="entry name" value="SSF160443"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="SSF48334"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01069">
        <property type="entry name" value="mutS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50828">
        <property type="entry name" value="SMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 7" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Smr" evidence="10">
        <location>
            <begin position="720"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="688"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="512"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="688"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="331"/>
            <end position="338"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ84090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="795" mass="88109" checksum="4F57E3671EE3B24B" modified="2013-07-24" version="1">MNPNERTLELDKILELLANEAANDLTREQARHLQPQTDLAVVQAELEKTDSALALAVQFGTPPFYAFQDMCTSLQRAKSGAKLSLKDLLEIARILRQVQALSDWYDHCSGVETPLDDLFSRLAPVPFLLEKLDRCILSEEELADAASPALADIRRKILRSGQRLRETLDKMVRNQDVQKCLQDARVTMRDGRFVLPVKAEHRGQVQGLIHDTSASGQTLFIEPLAVVEANNDMRLLESLEQEEIERIITELCADCGTWADAIIADHAVCAELNLYFAKANLAAKMHAFRPIVTDDGVLDLKNARHPLIPAEKVVPISFSLGREQKTLIITGPNTGGKTVALKTAGLLTLMAMCGLLIPAAEGSQISVFETVFANIGDTQSIEQNLSTFSAHMKQVIKILQQADDRSLVLLDELGSGTDPVEGAALAVAIIERLHNNGAKMMITTHYQELKLYATEHPEVENASCEFDLVHLKPTYRLILGSPGKSNAFAITEGLGMDPSIIQQAQTLVSEENVRFERAVEKLEQTRQTLEEQLQAIQVEKQQADEAAAKAKQELEEIRAERAEKIEQARLQAMQIVEQTRAESNALLEELDKLRKEKERATFSEDVISAKSSSKRRFRHMYETANPLEEAAATDASYQLPRALKVGDTVMVADTKQKGKVVSLPDSSNRLLVQFGAMKTKMQLERLRLVNSQPEKQPQKKNAPRTGKVSAKTERSGSMELDIRGCTCDDGVYQMDAFLDRAVLSHVSTVTIIHGKGTGLLRKAVHQRLKQLKYVKSFRLGVFGEGEDGVTIVTLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1I2</accession>
    <name>R5V1I2_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GTP cyclohydrolase 1</fullName>
            <ecNumber evidence="6">3.5.4.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">GTP cyclohydrolase I</fullName>
            <shortName evidence="6">GTP-CH-I</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">folE</name>
        <name evidence="8" type="ORF">BN566_01846</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>GTP + H2O = 7,8-dihydroneopterin 3'-triphosphate + formate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17473"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58462"/>
            <dbReference type="EC" id="3.5.4.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 6">Cofactor biosynthesis; 7,8-dihydroneopterin triphosphate biosynthesis; 7,8-dihydroneopterin triphosphate from GTP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homopolymer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the GTP cyclohydrolase I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.16" evidence="6"/>
    <dbReference type="EMBL" id="CBAR010000187">
        <property type="protein sequence ID" value="CCZ84120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01846"/>
    <dbReference type="EnsemblBacteria" id="CCZ84120">
        <property type="protein sequence ID" value="CCZ84120"/>
        <property type="gene ID" value="BN566_01846"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00848">
        <property type="reaction ID" value="UER00151"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003934">
        <property type="term" value="F:GTP cyclohydrolase I activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035998">
        <property type="term" value="P:7,8-dihydroneopterin 3'-triphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.286.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00223">
        <property type="entry name" value="FolE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043133">
        <property type="entry name" value="GTP-CH-I_C/QueF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043134">
        <property type="entry name" value="GTP-CH-I_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001474">
        <property type="entry name" value="GTP_CycHdrlase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018234">
        <property type="entry name" value="GTP_CycHdrlase_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020602">
        <property type="entry name" value="GTP_CycHdrlase_I_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11109">
        <property type="entry name" value="PTHR11109"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01227">
        <property type="entry name" value="GTP_cyclohydroI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00859">
        <property type="entry name" value="GTP_CYCLOHYDROL_1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5 6 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 6" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="GTP_cyclohydroI" evidence="7">
        <location>
            <begin position="9"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ84120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="190" mass="20860" checksum="341119A3965763A6" modified="2013-07-24" version="1">MAIDTEAIAYHVKGILKALGEDPEREGLKETPERVARMYAEVFAGIAYSNHEIAEMFGKTFSKPETDAGQTVVTMKDISVFSYCEHHMALMYDMHVNVSYVPHGKVLGLSKVARICDMAAKRLQLQERLGQDIAEIIMEAAGTPDVGVVITGSHSCMTARGIRNVPAVTTTTTFCGAYETDVTLQQRVMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1I5</accession>
    <name>R5V1I5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ROK family protein (Putative glucokinase)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00335</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000225">
        <property type="protein sequence ID" value="CCZ84428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00335"/>
    <dbReference type="EnsemblBacteria" id="CCZ84428">
        <property type="protein sequence ID" value="CCZ84428"/>
        <property type="gene ID" value="BN566_00335"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000600">
        <property type="entry name" value="ROK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18964">
        <property type="entry name" value="PTHR18964"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00480">
        <property type="entry name" value="ROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01125">
        <property type="entry name" value="ROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="313" mass="32931" checksum="1949CABF6CCB2B80" modified="2013-07-24" version="1">MKYYIGIDLGGTNIVAGVVNEAYEILAKASVKTNLPRPEQEIAADMAKVAKEAAEKAGLTMDQIEWVGIGTPGIANSETGIIEYSNNLGFVNTPMVKYIEDALGKKAFIENDANAAAYGEYVAGAAKGAKNAVCITLGTGVGGGIVIDGKIYSGSNFGGAEIGHTVISVDGPQCTCGRKGCFEVYSSATGLIRMTKEALAEHPDSMMKEEEHISGRTAFNYMRKGDAAAKEVVDKYIKYLAAGITNTLNIFQPDVLCIGGGVCNEGDPLLLPMKEIVARENYTRNSKKNAEIVIAKLGNDAGIIGAAFLGNAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1L1</accession>
    <name>R5V1L1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Dockerin type I repeat protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN566_00047</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 26 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000230">
        <property type="protein sequence ID" value="CCZ84448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00047"/>
    <dbReference type="EnsemblBacteria" id="CCZ84448">
        <property type="protein sequence ID" value="CCZ84448"/>
        <property type="gene ID" value="BN566_00047"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016985">
        <property type="term" value="F:mannan endo-1,4-beta-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006080">
        <property type="term" value="P:substituted mannan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002105">
        <property type="entry name" value="Dockerin_1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022790">
        <property type="entry name" value="GH26_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000805">
        <property type="entry name" value="Glyco_hydro_26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40079">
        <property type="entry name" value="PTHR40079"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00404">
        <property type="entry name" value="Dockerin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02156">
        <property type="entry name" value="Glyco_hydro_26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00739">
        <property type="entry name" value="GLHYDRLASE26"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51764">
        <property type="entry name" value="GH26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004406656" evidence="6">
        <location>
            <begin position="23"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="domain" description="GH26" evidence="7">
        <location>
            <begin position="179"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="8">
        <location>
            <begin position="562"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="532"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="533"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="353"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ84448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="625" mass="70341" checksum="C01AABD2457F3873" modified="2013-07-24" version="1">MHTWVRKGIALAVSGVLTIGMAASLPFSSLADAADEDIIFQAEGEDLDGATTWTDIYGQQFPGYSGSGFCYLTGETLTFEVEAPEEGMYEFTTRSAQILDKDGREQTISINGSEFMMKMPYADSWTDFSFGIHRLKKGTNKIQILPRYGYGAYDTITVKKADLPELSVSPTLSDSKATSETQGLMNYLCDVYGKHMLSGQQEIYGGGHTESSPNGYSGADLQGYETEFEYIKKNFGDYPAIRGFDYMNYNPLYGWDDQTTERIIEWGTERNGIPTVCWHINVPKNFANYELGDAVDWQKCTYKPDETDFDTSKAIVEGTKEYEYVMLTIKTLAAELKKVQDAGVPIIFRPYHEAEGNTNTDGSASWFWWGKSGAEVYKKLWKQLYTTLTEEYGIHNLIWEYNSYDYSTSPQWYPGDDCVDIVGYDKYNCVYNRHDGKTSGPNEDAISSTFYTLVNLTNGKKLVSMPENDTVPSLENIEIEKANWLYFCIWYDNGSDNFLSGTDKNNPETLKEMYQSDYCITLSELPDWKNYKNGGDTPTTTTATTDSGSETTTTVTTTTGTTEVVIGDVNSDGVINVVDAMLLKRYLLAEHAEDATYNTVWDWNQDETCDVLDVVGLTKFLLRKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1Q7</accession>
    <name>R5V1Q7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cobalt transport protein ATP-binding subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_01945</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01945"/>
    <dbReference type="EnsemblBacteria" id="CCZ84220">
        <property type="protein sequence ID" value="CCZ84220"/>
        <property type="gene ID" value="BN566_01945"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03225">
        <property type="entry name" value="ABC_cobalt_CbiO_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015856">
        <property type="entry name" value="ABC_transpr_CbiO/EcfA_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030947">
        <property type="entry name" value="EcfA_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04520">
        <property type="entry name" value="ECF_ATPase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="7">
        <location>
            <begin position="5"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="44"/>
            <end position="51"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ84220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="292" mass="32230" checksum="53941A2C46977C69" modified="2013-07-24" version="1">METQAQAKQLWFHYTDENGERTSPDILKDISLSIDKGDFVALLGHNGSGKSTFAKHMNAILLPSAGTMTVDGIDTKDESQRFTLRSHVGMVFQNPDNQIVATIVEEDVAFGPENLGIPPQEIRERVDDALKAVEMYDYREHAPSQLSGGQKQRVAIAGIIAMRPDCIVLDEPTAMLDPSGRREVMNTIHMLNREYGITIVLITHYMEEAAQANRVVVMDDGKVVLDNTPKEVFSHVETLKKIGLDVPQVTELAYRLQQAGCPVDVHMISEEECVASLTKLLNGKTGGAAWQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1S6</accession>
    <name>R5V1S6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00014</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000197">
        <property type="protein sequence ID" value="CCZ84245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00014"/>
    <dbReference type="EnsemblBacteria" id="CCZ84245">
        <property type="protein sequence ID" value="CCZ84245"/>
        <property type="gene ID" value="BN566_00014"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="94" mass="10702" checksum="65339095D3E9D7F8" modified="2013-07-24" version="1">MEQNFLFYKGYPLVRSGDCFYYGYMCEPHVIMIRVTKKDTSGVIPIASELQVYDLSTDENKSLRTAKRPSLYAALDLAATWLSRGLPDEQTQNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1U5</accession>
    <name>R5V1U5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Small GTP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00199</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000204">
        <property type="protein sequence ID" value="CCZ84270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00199"/>
    <dbReference type="EnsemblBacteria" id="CCZ84270">
        <property type="protein sequence ID" value="CCZ84270"/>
        <property type="gene ID" value="BN566_00199"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023873">
        <property type="entry name" value="FeFe-hyd_GTPase_HydF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041606">
        <property type="entry name" value="HydF_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040644">
        <property type="entry name" value="HydF_tetramer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18128">
        <property type="entry name" value="HydF_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18133">
        <property type="entry name" value="HydF_tetramer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03918">
        <property type="entry name" value="GTP_HydF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G" evidence="1">
        <location>
            <begin position="16"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="HydF_dimer" evidence="2">
        <location>
            <begin position="176"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="HydF_tetramer" evidence="3">
        <location>
            <begin position="278"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF18133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="404" mass="45025" checksum="3E7BE24F36566E65" modified="2013-07-24" version="1">MEAGLQQTPSADRIHIAFFGRRNAGKSSLVNAITNQTMSIVSEIQGTTTDPVRKAMELLPLGAVVIIDTPGLDDEGTLGSLRMQKTKEILHRTDLAVIVINSTIGKTELETKLEADLQQQQIPFLEVYNQCDLHMPEQLPDRAIAVSAKTGFHISELKEKMAVCYQKQAPKANRSLLGNFVQQDDIILLVTPIDESAPKGRMILPQVQAIRSILDEEAICLVVQPQQLQRTLLLLKQPPKLVVTDSQAFQQVNQIVPPEIALTSFSILYAHYHDVLQKAVTAAATLEYLQNHDQILISEGCTHHRQCNDIGTVKLPNWIQTYTGKTLDFTWTSGNAFPDDLTPYRLIVHCGGCMLQPRVIQYRKELAAKAGVPFTNYGTLIAYVHGILRRSVSIFPELQDFLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1X0</accession>
    <name>R5V1X0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Monogalactosyldiacylglycerol synthase C-terminal domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_00213</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000209">
        <property type="protein sequence ID" value="CCZ84300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00213"/>
    <dbReference type="EnsemblBacteria" id="CCZ84300">
        <property type="protein sequence ID" value="CCZ84300"/>
        <property type="gene ID" value="BN566_00213"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009247">
        <property type="term" value="P:glycolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009695">
        <property type="entry name" value="Diacylglyc_glucosyltr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007235">
        <property type="entry name" value="Glyco_trans_28_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04101">
        <property type="entry name" value="Glyco_tran_28_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06925">
        <property type="entry name" value="MGDG_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MGDG_synth" evidence="6">
        <location>
            <begin position="18"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_tran_28_C" evidence="5">
        <location>
            <begin position="208"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF06925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ84300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="383" mass="42512" checksum="E06D628CCFE70740" modified="2013-07-24" version="1">MNEHCNILILSVTAGNGHNSAAAALKEEFDRQGVNCHIMDLFQYLSPLAAKLISDGYDQLTKRFRLAWKIGYGVALHDTTFKIPPRLIASTSNLQSMQQYLIDQKITHVVFTHPFAGVLLQTLKQYQAIAIPTFGILTDFTIHPYWWDCTENDYFILPHADLIPAAEKNGFQRKQLKDFGIPVSSAFARHPEKMQVRTMLGMPVNLPTVLVMGGGVGYGNLVKTVQEIDEHAKHPLQTIVVCGRNTESRDELLQLQASGSFSHIVHIYGFTHQVSLLMAASDCIITKPGGITTSEALSASLPIVVSKPIPGQEEKNVEFLQQYDAICVPEKYRFSGMYAADLLFDSVRLKQLTQHAAQVAKPHAAADICSFVRECKSMVALRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1Y7</accession>
    <name>R5V1Y7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">50S ribosomal protein L13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rplM</name>
        <name evidence="5" type="ORF">BN566_00238</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself. It is important during the early stages of 50S assembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000213">
        <property type="protein sequence ID" value="CCZ84325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00238"/>
    <dbReference type="EnsemblBacteria" id="CCZ84325">
        <property type="protein sequence ID" value="CCZ84325"/>
        <property type="gene ID" value="BN566_00238"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00392">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01366">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005822">
        <property type="entry name" value="Ribosomal_L13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005823">
        <property type="entry name" value="Ribosomal_L13_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036899">
        <property type="entry name" value="Ribosomal_L13_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545">
        <property type="entry name" value="PTHR11545"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00572">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002181">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52161">
        <property type="entry name" value="SSF52161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01066">
        <property type="entry name" value="rplM_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ84325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="142" mass="15871" checksum="1E19BD72FF6829C6" modified="2013-07-24" version="1">MATTMPKAGEVSRKWYIIDAAGKPLGRVAAKAAHILRGKHKPTFAPHVDCGDFVIVINCDQAVLTGKKLEQKYYYRHSGWIGGLKQTQYKVMMEERSDEAMQIAIKGMLPHNSQGAAQLTRLRTYKGAEHKQAAQKPEVCEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1Z7</accession>
    <name>R5V1Z7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00414</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000244">
        <property type="protein sequence ID" value="CCZ84553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84553">
        <property type="protein sequence ID" value="CCZ84553"/>
        <property type="gene ID" value="BN566_00414"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="201" mass="23845" checksum="47FBBB78D34F6963" modified="2013-07-24" version="1">MQRNRTNRFQVFGDIFPHKSIATGRATGEDTVGIFQRNRKPVDFWLYHKFRIGKLLFAASQKRRYFCKRKDILQAHHLDGMLHLFKGTGCHTTYPLGRRGRQLQRGKRCFQFPQLPHHLIIRKIRNGRVIQYIIPIIMGFQFFGKLRNPFFGCCKFHGSSFLPVVDSAVFHYTTQRLKIQPDIRFFFQRRDICFLCPVIIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5V204</accession>
    <name>R5V204_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Membrane protein involved in the export of O-antigen and teichoic acid</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_00424</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000246">
        <property type="protein sequence ID" value="CCZ84563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00424"/>
    <dbReference type="EnsemblBacteria" id="CCZ84563">
        <property type="protein sequence ID" value="CCZ84563"/>
        <property type="gene ID" value="BN566_00424"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029303">
        <property type="entry name" value="Polysacc_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002797">
        <property type="entry name" value="Polysacc_synth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01943">
        <property type="entry name" value="Polysacc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14667">
        <property type="entry name" value="Polysacc_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="83"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="111"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="140"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="209"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="248"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="293"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="331"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="362"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="387"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="419"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="443"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="Polysacc_synt_C" evidence="7">
        <location>
            <begin position="335"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ84563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="473" mass="53512" checksum="14E90225931BD96D" modified="2013-07-24" version="1">MNQYKRLAANTMIFAIGSFGSKILTLFLTKLYTANINPADISTKELLEQTANFLIPIVTFSITDAILRYGLTRNYDKGKIFTSSYLVLGLGVCVMLALSPLMQLLPYTSGYLWLLMLYVFMSSLRSIHSQFVRARGMVTLFAFDGILATITLFIFNVIFIALLNMGITGFLLSVICSDFLSAVFLWWIADLRKYLHRKYISPKMTRLMIRFSIPLIPTTLLWLVTGFSDRLFLRYMDGPPNLVGDTAAGIYGIASKLPNLISTVSTVFYQAWNMSAILENDSANRGHFYKQVFSAYESFLVIASSVLIVFVRPFSDLLISTSTYTEYADAYRYTPILIISVFMMCLNQFFSSIYAASQHTSHSFWTSLVAAIVNLILNWLLIGALGISGAGFATFVSYLVSYLIRVVDTKRYVPFYIDHTRFVLNLILMFAMTFVTIQTPPAWHVIDGVLLTFTVLYNFKDLVSTVRRVLKRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>R5V224</accession>
    <name>R5V224_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA-specific adenosine deaminase</fullName>
            <ecNumber evidence="3">3.5.4.33</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">tadA</name>
        <name evidence="5" type="ORF">BN566_00444</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the deamination of adenosine to inosine at the wobble position 34 of tRNA(Arg2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>adenosine(34) in tRNA + H(+) + H2O = inosine(34) in tRNA + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:43168"/>
            <dbReference type="Rhea" id="RHEA-COMP:10373"/>
            <dbReference type="Rhea" id="RHEA-COMP:10374"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:82852"/>
            <dbReference type="EC" id="3.5.4.33"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="3">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytidine and deoxycytidylate deaminase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.33" evidence="3"/>
    <dbReference type="EMBL" id="CBAR010000247">
        <property type="protein sequence ID" value="CCZ84583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00444"/>
    <dbReference type="EnsemblBacteria" id="CCZ84583">
        <property type="protein sequence ID" value="CCZ84583"/>
        <property type="gene ID" value="BN566_00444"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052717">
        <property type="term" value="F:tRNA-specific adenosine-34 deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002100">
        <property type="term" value="P:tRNA wobble adenosine to inosine editing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00972">
        <property type="entry name" value="tRNA_aden_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016192">
        <property type="entry name" value="APOBEC/CMP_deaminase_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028883">
        <property type="entry name" value="tRNA_aden_deaminase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14437">
        <property type="entry name" value="MafB19-deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="SSF53927"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00903">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="2 3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="4">
        <location>
            <begin position="6"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="3">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ84583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="162" mass="18110" checksum="6C76E2403CF4E9A2" modified="2013-07-24" version="1">MQELYVPQERYMRRALELAEYAASLGEVPVGAVVVHKPMNRIIGEGWNLRESGKSPLAHAEIMAIDQASRTLGGWRVIDSAIYVTLEPCPMCCGAILHARLDHLVFGAYDPKGGAVASVESMFDLPYNYHPTIEGGFLQEDCATVLTQFFRELRRKKKEQAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5V231</accession>
    <name>R5V231_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyltransferases involved in cell wall biogenesis</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00282</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000222">
        <property type="protein sequence ID" value="CCZ84375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00282"/>
    <dbReference type="EnsemblBacteria" id="CCZ84375">
        <property type="protein sequence ID" value="CCZ84375"/>
        <property type="gene ID" value="BN566_00282"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="268"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_trans_2-like" evidence="2">
        <location>
            <begin position="6"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="321" mass="36412" checksum="E19E28FB0C9B5BC2" modified="2013-07-24" version="1">MQKLYLVIPCYNEEEVLPETSKQLRAKMETLMAAGKISKESRIVFVNDGSKDRTWEIIESLHKENPLFQGIKLSRNKGHQNALLAGLMTVKEHCDMAISMDADLQDDIDAIDGMLEKYAEGCDIVYGVRNARDTDTAFKRKTAEGYYKVMRLLGADIVYNHADYRLMSKRALDGLEQFKEVNLFLRGVVPMIGYKSDKVYYARKERFAGESKYPLKKMLAFAWEGLTSLSVKPIRMIMTLGSVIFLVSIAMLIYSVVRHAMGATMVGWTSTVVSIWAIGGLQLLAIGIIGEYIGKVYLETKQRPKFIVEEYLKDEDAEKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5V275</accession>
    <name>R5V275_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Permeases of the drug/metabolite transporter (DMT) superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00491</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the EamA transporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000257">
        <property type="protein sequence ID" value="CCZ84638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00491"/>
    <dbReference type="EnsemblBacteria" id="CCZ84638">
        <property type="protein sequence ID" value="CCZ84638"/>
        <property type="gene ID" value="BN566_00491"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="40"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="125"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="180"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="210"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="240"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="264"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="3">
        <location>
            <begin position="8"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="291" mass="32572" checksum="749A74F92BD910E9" modified="2013-07-24" version="1">MQLSSRTKGTCFLIFSAFSFAWMNAFVRLAGDLSFIQKSFFRNLIALLVALIMLFKERQPLHCDKKSAGALLLRATMGTVGIFCNFYAVDHLPISDASMLNKMSPFFSVLLAWLLLKERITWKQICIICGAFIGSLFIIKPTFSNLSLFPSCIGLLGGFGAGFAYTMVRYLGTRHVPKAWIIFLFSAFSCLVALPWLLLHYEPMTWKQLLSLIGAGLAAAGGQFGVTNAYFYAAPKDISVYDYTQIIFAALLGFFLFQQVPDRYSILGYCIIIGMAVCMFFYHKRAESHTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5V297</accession>
    <name>R5V297_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Alkyl_sulf_C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00521</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000267">
        <property type="protein sequence ID" value="CCZ84668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00521"/>
    <dbReference type="EnsemblBacteria" id="CCZ84668">
        <property type="protein sequence ID" value="CCZ84668"/>
        <property type="gene ID" value="BN566_00521"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029229">
        <property type="entry name" value="Alkyl_sulf_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036527">
        <property type="entry name" value="SCP2_sterol-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14864">
        <property type="entry name" value="Alkyl_sulf_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55718">
        <property type="entry name" value="SSF55718"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alkyl_sulf_C" evidence="2">
        <location>
            <begin position="25"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="228" mass="24071" checksum="0BD39E1021687074" modified="2013-07-24" version="1">MTFFEMFDAAKAGFANADVSGLNGHVAIQIEVTEDGCGIFYAEVTDGVLNVQPYDYRDNTAAVTLPHSTLFALLRRETTLPEAVAQEKAFVQGCMESAAKLFAAVPAPKKAEAPKKEAAPAEEKKPACCKATAAETKKETTCKAAATPVIEEKKEAATTPVKATAPVVEEKKEAAATTAKAAAPVVEEKKEAATATTTTTTAPKTAAAKTVNRKATSKPMTKHNNRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5V2F8</accession>
    <name>R5V2F8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00384</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000240">
        <property type="protein sequence ID" value="CCZ84485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84485">
        <property type="protein sequence ID" value="CCZ84485"/>
        <property type="gene ID" value="BN566_00384"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="65" mass="7497" checksum="EC77CB0F5B7123C8" modified="2013-07-24" version="1">MTVKQSAAQIHTFYIIKDFGTFCNAESSDFLSLWNKSYFLHKMCGLFLCILFLFPKFTTKGCLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5V2M8</accession>
    <name>R5V2M8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">30S ribosomal protein S8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpsH</name>
        <name evidence="7" type="ORF">BN566_00071</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the 30S ribosomal subunit. Contacts proteins S5 and S12.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the universal ribosomal protein uS8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000241">
        <property type="protein sequence ID" value="CCZ84525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00071"/>
    <dbReference type="EnsemblBacteria" id="CCZ84525">
        <property type="protein sequence ID" value="CCZ84525"/>
        <property type="gene ID" value="BN566_00071"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01302_B">
        <property type="entry name" value="Ribosomal_S8_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000630">
        <property type="entry name" value="Ribosomal_S8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035987">
        <property type="entry name" value="Ribosomal_S8_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11758">
        <property type="entry name" value="PTHR11758"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00410">
        <property type="entry name" value="Ribosomal_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56047">
        <property type="entry name" value="SSF56047"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00053">
        <property type="entry name" value="RIBOSOMAL_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ84525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="132" mass="14374" checksum="6792FDD62BBEE94B" modified="2013-07-24" version="1">MQITDTIADILTRIRNASSAKHATVDVPASNVKKAITQILLDEGYIKSFQVVEDGKQGIIRITLKYTDSKAPVITGLRRVSKPGLRIYSSCADMPKVRKGLGIAIVSTSKGIMTDKKARELNVGGEVLAYVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5V2S7</accession>
    <name>R5V2S7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00436</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000247">
        <property type="protein sequence ID" value="CCZ84575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84575">
        <property type="protein sequence ID" value="CCZ84575"/>
        <property type="gene ID" value="BN566_00436"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="607"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="607"/>
            <end position="622"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="805" mass="87046" checksum="79BA717EDBD16093" modified="2013-07-24" version="1">MDDFKNNLPESDHTGFAPPQNDATPTSTPANDAAAASAANPFQQPAPSADATTTPPQPETPPSFSQTPPPFQQPDFSLNGQIVGAKSKKKMKGWKIAVISVISAVVVLGAGTGIAYAASDTVKNFAKMNFSSESDYYQWVIEKNTKDWGRSVSDRYDSSLSRSKEEPQSNLSMNITLSEEGQKKLQEELNSDVDTDETITLTSAAIAFSGKAEADKMGGTVTITLNGEDVLEGNAVMDANNIYLQIPSLSDQYLSMTWQDVETLTEEEYDLSDEFDSSIDSSALNSSSLFLTPEVLEDRISPSDLNSMFQRYGEIIADSDATIALLKNQELNIADTTFSYTALEATYQGDELKTVVKELLEEVKEDSTISHYFAASDLMTEDEFQSNLQEALDNWNDTTVTESATVTLYVDPTGTIRGCRIADPDDADSSLLECYAGKDGKQIGVYFDVNGLNVSGVLSEGSKDTYSGTITVALEENSVLLDLEDFKIVDDERGYCSGSLTASTEDEPNLFTLSLDSDGKSQTFSVDWAVGGTNYATISCTLSTDKGDDLALNIPSDSLDLSDDATKDAYFGDGTQTKDCLKKIATALGIQDADNFADSIYDEINGGDDLNTQSSDSDITTLPADSDRPVEDGVETEVPTDTTDTTDSSDNEQRIDADLSIAAITYNGGKLFRFYENNMDLIKLVDCSVDPTIESQDYNWYSNSDATLTLGLENMSTSPTDAQACNIFEVEVYEGAPSDIRVGVVGIGSSVADLEAQFNVDIVPEAETASSYVVSIYDTVSNGQKAYSFYIENGRVTAFDLVDYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5V2U0</accession>
    <name>R5V2U0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00451</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000250">
        <property type="protein sequence ID" value="CCZ84590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84590">
        <property type="protein sequence ID" value="CCZ84590"/>
        <property type="gene ID" value="BN566_00451"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="233" mass="27429" checksum="B21E041034FF4906" modified="2013-07-24" version="1">MANRSETDKAHEKICEVTAEYFFTHKKEAFFSLSTQEIYEEVKKYASNAVNYATLKFPGFLQVRKIWISFLSENHQYTPGERLTVAVLYRLIYIYKYYDYGIIKDILEYNHYHVQMHDGDPIICVIPLLPEKISDIYAKNIIHGKKEAMIRWNFIRNLCKEIKKINPSLILAVIPQVNRMSFMCKTGKINCQIHDLPPIYDSLCIFIKNTPEGQNLIRNFPISHSALERKRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>R5V315</accession>
    <name>R5V315_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Signal recognition particle protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Fifty-four homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ffh</name>
        <name evidence="11" type="ORF">BN566_00523</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="7">The SRP-RNC complex is targeted to the cytoplasmic membrane.</text>
    </comment>
    <comment type="domain">
        <text evidence="7">Composed of three domains: the N-terminal N domain, which is responsible for interactions with the ribosome, the central G domain, which binds GTP, and the C-terminal M domain, which binds the RNA and the signal sequence of the RNC.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the GTP-binding SRP family. SRP54 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000267">
        <property type="protein sequence ID" value="CCZ84670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00523"/>
    <dbReference type="EnsemblBacteria" id="CCZ84670">
        <property type="protein sequence ID" value="CCZ84670"/>
        <property type="gene ID" value="BN566_00523"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048500">
        <property type="term" value="C:signal recognition particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00306">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036891">
        <property type="entry name" value="Signal_recog_part_SRP54_M_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004125">
        <property type="entry name" value="Signal_recog_particle_SRP54_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004780">
        <property type="entry name" value="SRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022941">
        <property type="entry name" value="SRP54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564">
        <property type="entry name" value="PTHR11564"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564:SF7">
        <property type="entry name" value="PTHR11564:SF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02978">
        <property type="entry name" value="SRP_SPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47446">
        <property type="entry name" value="SSF47446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00959">
        <property type="entry name" value="ffh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00300">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 7" id="KW-0733">Signal recognition particle</keyword>
    <feature type="domain" description="SRP54" evidence="10">
        <location>
            <begin position="268"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="447"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="305"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="108"/>
            <end position="115"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="189"/>
            <end position="193"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="247"/>
            <end position="250"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ84670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="468" mass="51538" checksum="639BD37834E8A048" modified="2013-07-24" version="1">MAFEGLSDKLGNVFKKLRSHGKLTEADVKEAMREVRMALLEADVSYKVVKDFVAKVSARAVGEEVLNSLTPAQQVVKIVNEELCALMGNNNARINFPSKPPCVIMFCGLQGSGKTTHAAKLAKMLKKEGHYPLLVACDIYRPAAIHQLQVVGERAGVKVFEMGQISPMIIAKEAMKFAKDHGHDVVILDTAGRLHIDLELMEELKQLKELTHPDEIMLVVDAMTGQDAVNVAKAFDDALGIDSVLMSKLDSDTRGGAALSVLAVTGKPIKYVGMGEKLDDFEPFHPERMASRILGMGDVLTLIEKAESTVNEKDARKLAQKLEENKFDMNDLLDQLRQIQKMGSLKSIIGMLPGVGDKMKDVDLDDRQFVRIEAMITSMTKAERAKPSIINPSRKRRIAAGSGTKVEEINRLLKQFEQMQKMMKQFGSKGGKLGRRARKQLAGMNLEQLQNSIPGNIPGVPPGLMPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5V3X6</accession>
    <name>R5V3X6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted xylanase/chitin deacetylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00619</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000009">
        <property type="protein sequence ID" value="CCZ82814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00619"/>
    <dbReference type="EnsemblBacteria" id="CCZ82814">
        <property type="protein sequence ID" value="CCZ82814"/>
        <property type="gene ID" value="BN566_00619"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045493">
        <property type="term" value="P:xylan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="SSF88713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51677">
        <property type="entry name" value="NODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="2" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0858">Xylan degradation</keyword>
    <feature type="domain" description="NodB homology" evidence="1">
        <location>
            <begin position="96"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="281" mass="31984" checksum="3A1CDAF37B743D94" modified="2013-07-24" version="1">MHRSHKGIWFLILDGCLILLAFLLTKGCPQTVHAETPVSLPVLMYHSIAPGTESDYQIRPETFAADLSYLNDAGYTSVSSAELIRYVNGTGDLPEQPILLTFDDGFYNNYSLALPLLEQYDMCAIVSVVGQFTDELAPESPHEDSYSYLTWEDLQALLDSGRIELGSHTYQMHNHTDRCGCRIQENEPEEQYHDTLLSDLGLLQTRFQEELQIEPVVFAYPYGFLCSESNPVLRELGIQMTLNCREQGNMITRDPECLYGLNRYNRFGNCETAVWMQRIAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5V4E8</accession>
    <name>R5V4E8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Amino acid transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_00779</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000034">
        <property type="protein sequence ID" value="CCZ82979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00779"/>
    <dbReference type="EnsemblBacteria" id="CCZ82979">
        <property type="protein sequence ID" value="CCZ82979"/>
        <property type="gene ID" value="BN566_00779"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="9"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="154"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="191"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="222"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="275"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="320"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="346"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="385"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="416"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ82979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="471" mass="51135" checksum="6F9EF163D30D61FF" modified="2013-07-24" version="1">MAAQQEKKLGLGSVVSISVGLIIATSCLVSLGQGAGTIGVTFIFAMVIACILNMTTVASLSELNALMPNTTGGLAQYTLACMGPFPTIISMVGGYILCNVLSCGVEASIFSYAMSDTFSFLHIPSLGYVLIVTTLLMIANLFGVDIFAKLQDVVAFLLVGSMLLMGIIGAIGIGFGKKIDQPYTMATDFKSIVSMTAVAFWLFIGAEYVIPVSKSVKNAKRNVPLGMMIGLGLICIVQSILVFGFHNYVPWAELADSPAPHLLYGMNLLGTFGKIWMTLVAAFAVISTQNSTVNALASICQGMAKMNMMPQVFAKTNRRGVPYVGVVFVSVMIFVFSALSNNSSDAISFLILVGSVFWMISYILAHMDVLILRKRFPKAPRSFKVPFGPAIPVIGILGTVYMILNISTDTAERNAIWLVTGGTFLLLGIYAFFWIKYKMKMPVFKSVPMEKVMAMENDMYYTIRKRRGIWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5V4G3</accession>
    <name>R5V4G3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Multidrug export protein MepA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_00782</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family. MepA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000036">
        <property type="protein sequence ID" value="CCZ82989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00782"/>
    <dbReference type="EnsemblBacteria" id="CCZ82989">
        <property type="protein sequence ID" value="CCZ82989"/>
        <property type="gene ID" value="BN566_00782"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13143">
        <property type="entry name" value="MATE_MepA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045070">
        <property type="entry name" value="MATE_MepA-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006603">
        <property type="entry name" value="DinF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0046">Antibiotic resistance</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="54"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="90"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="166"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="190"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="233"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="270"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="311"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="359"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="391"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="415"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ82989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="452" mass="48892" checksum="9345E058FF8F90EC" modified="2013-07-24" version="1">MTKTIRPPSTLSAFLHYVSLNMLAMLGTSCYILADTFFVANGIGEDGLTALNLVLPLFNLMNATGLMIGIGGATKYAIHKARGEQEAGSQIFFHALLLSGVASVGFMLMGLLGADPICRWLGSNAATHDKMVVYLRTLYCFAPLFLCNQLFAAFVRNDGAPNRAMLGTMLGTLGNIVLDYLFIFPLGMGMLGAVLATACAPLIGITIQSFYFRKPTCQIRVRPCRLRWQMFQPIFLLGGAEWITEVASGIVIFCFNFVVLRKAGNTGVAAYGIIANIALVAACLCTGIAQGVQPLLSRSYGKSDLPEMKKLLRMTICTALGFAAVLYGVLFGFTEPIVRLFNSTGNSTLQAMAETGTRIYFVAFCFVGVNIATSAFFAAVDRPAEAFCISLLRSGVLLLPLVFLFAAVWDMNGVWASYPVAELGTCLVAVCCQVRYWQNQQKQKGKIVEDDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5V4W2</accession>
    <name>R5V4W2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cobalamin synthesis protein P47K</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_00936</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIMIBI class G3E GTPase family. CobW subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000064">
        <property type="protein sequence ID" value="CCZ83149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00936"/>
    <dbReference type="EnsemblBacteria" id="CCZ83149">
        <property type="protein sequence ID" value="CCZ83149"/>
        <property type="gene ID" value="BN566_00936"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011629">
        <property type="entry name" value="Cbl_biosynth_CobW-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036627">
        <property type="entry name" value="CobW-likC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003495">
        <property type="entry name" value="CobW/HypB/UreG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02492">
        <property type="entry name" value="cobW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07683">
        <property type="entry name" value="CobW_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="cobW" evidence="3">
        <location>
            <begin position="5"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="CobW C-terminal" evidence="4">
        <location>
            <begin position="287"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="211"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="374" mass="41737" checksum="87988A3BDB196E2E" modified="2013-07-24" version="1">MTKVDIFSGFLGAGKTTLIKKLIAEAYAGEQLVLIENEFGEIGIDGGFLQDAGVNITEMNSGCICCSLVGDFGNALQKVLDQYHPDRILIEPSGVGKLSDVIRAVQNLHNAEIHLESFTTVVDAKKCKMYQKNFGEFFNNQIEHASCLILSHTAGLAESKLEDCVQRLRALNTTAPIVTTDWEQLSGEQLLAAMNQTETLDSALKNLLEETEHEHEHDHEHEHEHHHHDHDDHDHDESCGCHDHDHHHDHDEHDHDHDHGESCTCGCHDHDHDHEHHHHHHADEVFTSWGMETAKTYTAEEIQTALEQLEDAEKYGMILRAKGIVAGADGKWIHFDYVPGTPDVRTGSASTTGRLCVIGSHIQEDALAALFRTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>R5V519</accession>
    <name>R5V519_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01002</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000074">
        <property type="protein sequence ID" value="CCZ83219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83219">
        <property type="protein sequence ID" value="CCZ83219"/>
        <property type="gene ID" value="BN566_01002"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="294" mass="33711" checksum="86794F161FCC315E" modified="2013-07-24" version="1">MELLQLEYFYAVAKTQHVTHTAEQLHIAQPALTQSIHRLEKELGVPLFHRSGRNIALTEYGVYLRDALKPILENLHELPEVLQEMANVRKRTIRVNVLAASTLVTHALISYQKSHSGLNFRLIQNSQCEDADITIFTRSFFQQPADGRGRYQIFTERIFIAVPQDSPYARCESVRLKDFAHQNFISLSGSRSIRSICDRYCQHAGFTPNIIFESDSPDTVKNLIAGGLGVGFWPHYTWGQENMDQIVLLPISEPTCQRDLVVHLHRHAVEHAEAVDFFQFLSRYLLELKGQKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5V5I5</accession>
    <name>R5V5I5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Secretion protein HlyD family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01170</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000094">
        <property type="protein sequence ID" value="CCZ83394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01170"/>
    <dbReference type="EnsemblBacteria" id="CCZ83394">
        <property type="protein sequence ID" value="CCZ83394"/>
        <property type="gene ID" value="BN566_01170"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004406727" evidence="3">
        <location>
            <begin position="26"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="148"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="106"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="585" mass="64732" checksum="1ABD9F0DB43C28C5" modified="2013-07-24" version="1">MKRKKILAISIVCVLLVGGGSVGGAAYYKKSKNEKRVVNVTPVVNMMGYDYGSEKTIDGTVITGSKQNVMLEKNQVVQSVLVKKGDKVTVGTPLIAYDTTALKLAVQEKKNAVDAAEDDLRQARKELERLQSLHPAEDQVYDMIDDYDEPEEPDLEEDPTAGEVVAPVFPTTEPTTTDETEPTGEESTTESTDVTEAPTEEPTQPDDGHLHSIMKDASQGDGSMEKPYDFYCESDTIVGADLLLELQQSSSYARFIVPGDGYRWLVQGSALPETLTDWAVNQGISVAEDGMVSVDFKQVSFGIFQMQGTGGSSSAEDWGDEDFDFDDDFWDDSDSGGESEDYMYSAQELSNMIQKQQSKIKTLEFTKKSADLDYEVAQKKQDDGMERSLIDGVVTEIHSDLTEKEAETKPYLVIQGSGGLQVKGTISEWNLNQLQIGTEIQAMSYDTGDSFTMTVTGVDDIPETDGSAPYDENPSSSVYTFEAETSEQHDIAVGSWLSLSLQEAADTSTFYLPLQYVRKENGQYYVMKANDQNRLEKQWIQTGKIRYGSEIEIKSGVSETDRLCFPYGKDVKEGVRTKDTDDVQW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5V5Y4</accession>
    <name>R5V5Y4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01273</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000119">
        <property type="protein sequence ID" value="CCZ83504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83504">
        <property type="protein sequence ID" value="CCZ83504"/>
        <property type="gene ID" value="BN566_01273"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="184" mass="20283" checksum="E456735E33C17B5C" modified="2013-07-24" version="1">MNWYARWDESANDMVERDASTLQFSAVGNAAAPVTTTTRATTRPTTTTTTTTTKKTTTTKKATTTKKTTATTKKTTTTTKTTTTTTKPSSGKKVVLKNVSYRTAFDLTPYNNQKIRFITLQFQGSIPNSGGCLVLGNCWNEQHPIAYSDLQNGTITFQIQNPQDVMTIYNYYGLGSLQAVTLNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>R5V611</accession>
    <name>R5V611_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Nuclease SbcCD subunit D</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">sbcD</name>
        <name evidence="12" type="ORF">BN566_01298</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'-&gt;5' double strand exonuclease that can open hairpins. It also has a 5' single-strand endonuclease activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 9">Heterodimer of SbcC and SbcD.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the SbcD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000121">
        <property type="protein sequence ID" value="CCZ83529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01298"/>
    <dbReference type="EnsemblBacteria" id="CCZ83529">
        <property type="protein sequence ID" value="CCZ83529"/>
        <property type="gene ID" value="BN566_01298"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00840">
        <property type="entry name" value="MPP_Mre11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041796">
        <property type="entry name" value="Mre11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004593">
        <property type="entry name" value="SbcD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026843">
        <property type="entry name" value="SbcD_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12320">
        <property type="entry name" value="SbcD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00619">
        <property type="entry name" value="sbcd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7 9" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="9" id="KW-0235">DNA replication</keyword>
    <keyword evidence="9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6 9" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos" evidence="10">
        <location>
            <begin position="1"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="SbcD_C" evidence="11">
        <location>
            <begin position="265"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF12320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CCZ83529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="379" mass="43400" checksum="07D7516ED0AC8CEC" modified="2013-07-24" version="1">MNILHMSDLHLGRRFYNLSFLPIQQALLQEVLQIIDDQQVTVLLLCGDIYDKPVPPADAVQLLDWFLTQLAERSVHVCMISGNHDSPERLQFGSDLLQKNQIHISGTFSGKMETVSLSDDYGTLTISMLPYVKLAQLSVQFPEQSFATLDDGIRAVLEQIPPDPNHRNLLLYHGFVLHNGAAPEESDSETQLGGLQLVSDTLFDAYDYVALGHIHKPQWVQHDRIRYSGSLMKYSFSECLQQKSVTLLKWNEKDNCTITTIPLHPKQEMRILRGTLEQLLEHATPSEDFIRAELTDLELIPNAIEKLRVVYPNTLELSYIERERLRQPAAATEAVHVEEQSLLDLVTEFYENVYHYPLREHPEELALMTELVEEEQESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5V696</accession>
    <name>R5V696_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Helix-turn-helix family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01380</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000147">
        <property type="protein sequence ID" value="CCZ83644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01380"/>
    <dbReference type="EnsemblBacteria" id="CCZ83644">
        <property type="protein sequence ID" value="CCZ83644"/>
        <property type="gene ID" value="BN566_01380"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="144" mass="16061" checksum="FDD83E7F146EE851" modified="2013-07-24" version="1">MYNTQLTYDRIDEMSKLRGISIGKINEICSLSKNAISNAAKSEYGMKAKNIVLISEILDVSTDCLLGRTDNPNVSGNNIIQSSNVINGNNGNHSPLTVTETEKENNCKEIEVLLEKMPRSKQLRAIADVLDLLEEEYINEDDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5V739</accession>
    <name>R5V739_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GTP cyclohydrolase 1 type 2 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01643</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GTP cyclohydrolase I type 2/NIF3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000172">
        <property type="protein sequence ID" value="CCZ83914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01643"/>
    <dbReference type="EnsemblBacteria" id="CCZ83914">
        <property type="protein sequence ID" value="CCZ83914"/>
        <property type="gene ID" value="BN566_01643"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002678">
        <property type="entry name" value="DUF34/NIF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036069">
        <property type="entry name" value="DUF34/NIF3_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13799">
        <property type="entry name" value="PTHR13799"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01784">
        <property type="entry name" value="NIF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102705">
        <property type="entry name" value="SSF102705"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00486">
        <property type="entry name" value="YbgI_SA1388"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="265" mass="28822" checksum="2216BD777EB26DC7" modified="2013-07-24" version="1">MRTVQDVYSIINTFSPFSTQEKWDNSGLLVGTGSMPVHKVYVTLDISNETIAAAQELGADLMVAHHPIIFSPLKQLSPDSPVWKLAAANMAAICVHTPLDRARGGINDRLHQLLQEPLQLSGAMQTPEADCNGIGFGWADQSNIDWNTKDLAEALRCVLGCTVVRYAPIDRPIRKIYVGCGSCASMLEDAAAWGCDAMITGDVKHDRWYAAKNLGIALFDCGHYHTEQIAAQILTEQIQAALPDLPCICDPHGDPVCYAMGGEQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5V766</accession>
    <name>R5V766_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_01673</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000174">
        <property type="protein sequence ID" value="CCZ83944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01673"/>
    <dbReference type="EnsemblBacteria" id="CCZ83944">
        <property type="protein sequence ID" value="CCZ83944"/>
        <property type="gene ID" value="BN566_01673"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="300" mass="33904" checksum="FB9ED285EEA4BA35" modified="2013-07-24" version="1">MILAHQLTKRFEDFTALRSLSCEIEKGCIYGMVGSNGAGKSTFLRLLTGVYQPDEGSISIDGKPVWNNPEVKQHIAYVPDELYFLNGASMNRMADLYAACYPSFDRSYYQQRTKQFELNPKKSLNTFSKGMRRQAATILALSIRPDYIFFDETFDGLDPVMRNLVKKLICEDVLERNATAIITSHSLRELEDTCDQLALLHKGGLVLESDVQNLKTEQFKIQIAFPDDYGESRFTAIPDMKIRHFSKQGAVANLIVTGNRETIVAALQAMHPTILDVLPLSLEEVFTYEMEALGYAFDIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7C3</accession>
    <name>R5V7C3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Uridylate kinase</fullName>
            <shortName evidence="6">UK</shortName>
            <ecNumber evidence="6">2.7.4.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Uridine monophosphate kinase</fullName>
            <shortName evidence="6">UMP kinase</shortName>
            <shortName evidence="6">UMPK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">pyrH</name>
        <name evidence="8" type="ORF">BN566_01718</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the reversible phosphorylation of UMP to UDP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>ATP + UMP = ADP + UDP</text>
            <dbReference type="Rhea" id="RHEA:24400"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.22"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="6">Inhibited by UTP.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 6">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; UDP from UMP (UMPK route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the UMP kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.22" evidence="6"/>
    <dbReference type="EMBL" id="CBAR010000178">
        <property type="protein sequence ID" value="CCZ83989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01718"/>
    <dbReference type="EnsemblBacteria" id="CCZ83989">
        <property type="protein sequence ID" value="CCZ83989"/>
        <property type="gene ID" value="BN566_01718"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00159">
        <property type="reaction ID" value="UER00275"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033862">
        <property type="term" value="F:UMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04254">
        <property type="entry name" value="AAK_UMPK-PyrH-Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01220_B">
        <property type="entry name" value="PyrH_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011817">
        <property type="entry name" value="Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015963">
        <property type="entry name" value="Uridylate_kinase_bac"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005650">
        <property type="entry name" value="Uridylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02075">
        <property type="entry name" value="pyrH_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 6" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AA_kinase" evidence="7">
        <location>
            <begin position="6"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="11"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="132"/>
            <end position="139"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="236" mass="25362" checksum="729B4744E8784C2B" modified="2013-07-24" version="1">MGLKYKRVVLKLSGEALAGNQKTGLDFNVINEICASIKKCVDAGAEIGIVVGGGNFWRGRSSGNMDRTRADHIGMLATTMNALAVADAMEALGVDVRVQTAISMQQIAEPYIRNKAISHLKKGRVVIFGCGTGSPFFSTDTAATLRAVEIEADILLKATMVDGVYDKDPHKYTDAVRYEKITFSDVLSSALAVMDSTAASMCRDNKMPMLVFDLNRPDNIYDAIMGEPVGTVVSEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7V7</accession>
    <name>R5V7V7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">MurB family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_01929</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01929"/>
    <dbReference type="EnsemblBacteria" id="CCZ84204">
        <property type="protein sequence ID" value="CCZ84204"/>
        <property type="gene ID" value="BN566_01929"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007516">
        <property type="entry name" value="Co_F420_Hydgase/DH_bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007525">
        <property type="entry name" value="FrhB_FdhB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007345">
        <property type="entry name" value="Polysacch_pyruvyl_Trfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04432">
        <property type="entry name" value="FrhB_FdhB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04422">
        <property type="entry name" value="FrhB_FdhB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04230">
        <property type="entry name" value="PS_pyruv_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="397"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="433"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ84204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="915" mass="103709" checksum="985463E11D45400B" modified="2013-07-24" version="1">MSKKYDVGILGVWIGCNYGSIATYYALHQVIASMGKSILMIDKPILSENDVERKETHARRFGREHYHISKQYCLEDLKELNELCDGFVIGSDQVWNYGISRDFGKMFYLDFAEEQKKKIAYAVSFGHDVDFAPAEERVKIADYMSRFDGISVREADGVRLCRDDYGILAKQVLDPVFVADPSIYQGLIEKSSHREEEPFLASYILDPTPEKREALLHLSKELGGIKIINMLDGLPWLFEKNRKLMNLPNCVENLQVEDWLYYLSHAKFVVTDSCHGASFALIFKKNFIAIANKKRGYSRFKSLAELMHFESHLVTDVNQILTDKKLLEPIDYAAVEATILSERSRCYAWLQEILNQPKKTTETLKLENIIGSPNSNSPHAISVLNRKPIQKKSISASAITSNLPVIECAGCGACVSICPVKALELKPDEHGLYRAVLNEKTCINCGKCLKVCPSYRLPSNANTTTPNCYAVIASDAELLKKSSSGGVFSLLAKKAFEQQGAVGGAAWKEDLSVEHILIDQESDMPKLRKSKYLQSYLGNIDAQVKKRLQQGQFVLFSGCPCQIAGLRAYLGKDYENLITIDLLCGNSPSAGFFQKYLQDAFGDQVASYEFRNKAQGWNSDCVSIQLKSGEQIIRRGKAEDAYQSVYHNHTMCPPHCQNCKYQQLPRFGDITIGDFWGISKRMPEFDYTKGVSVVICNNEKGQAFFDQISEQEFTLKKEVPLKWIGGNGYALEGKHNYASPYRDAFFDKVRTEPFAKAVKLAFDQQNLGAIPSEFLNPLQFHSQQIGFQYDLYTWEQHLIQGRLVLIPKEQQSKPGNYASMPLAMHLQGGKRYHLFMRFKIQTESQWLNLHIKEAGSKRLQIIHRHKVQPNDAKEWVTISKDFVPNNDIYSEFMVGAMQIAGEGRFLAIDYIYITD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>R5V8B2</accession>
    <name>R5V8B2_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glutamate 5-kinase</fullName>
            <ecNumber evidence="7">2.7.2.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Gamma-glutamyl kinase</fullName>
            <shortName evidence="7">GK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">proB</name>
        <name evidence="9" type="ORF">BN566_00271</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + L-glutamate = ADP + L-glutamyl 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:14877"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58274"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the glutamate 5-kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.11" evidence="7"/>
    <dbReference type="EMBL" id="CBAR010000220">
        <property type="protein sequence ID" value="CCZ84364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00271"/>
    <dbReference type="EnsemblBacteria" id="CCZ84364">
        <property type="protein sequence ID" value="CCZ84364"/>
        <property type="gene ID" value="BN566_00271"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00359"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004349">
        <property type="term" value="F:glutamate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04242">
        <property type="entry name" value="AAK_G5K_ProB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00456">
        <property type="entry name" value="ProB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041739">
        <property type="entry name" value="G5K_ProB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001057">
        <property type="entry name" value="Glu/AcGlu_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011529">
        <property type="entry name" value="Glu_5kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005715">
        <property type="entry name" value="Glu_5kinase/COase_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019797">
        <property type="entry name" value="Glutamate_5-kinase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000729">
        <property type="entry name" value="GK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00474">
        <property type="entry name" value="GLU5KINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01027">
        <property type="entry name" value="proB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00902">
        <property type="entry name" value="GLUTAMATE_5_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 7" id="KW-0641">Proline biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AA_kinase" evidence="8">
        <location>
            <begin position="8"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="174"/>
            <end position="175"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="216"/>
            <end position="222"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ84364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="270" mass="28967" checksum="BE3AAC9E3AB16C02" modified="2013-07-24" version="1">MIDFTKKKRIVIKLGTSTLTHKTGKLNIRRMTNLVQVLSDLHNAGKEILLVSSGAIGMGVGKLNLPERPKDTPSKQAAAAVGQCELMHIYDDMFSKYSITVAQILLTKTIVQNPERRQHVQNTIERLLQLGVIPILNENDTVAIDELELEIGENDSLSAVIATVADADLLVILSDIDGLFSADPRVHQDAEVVPLVSAIDDSILQMAGGAGSTLGTGGMATKLNAAQIATENGIDMVIMNGKNPDLLYDLFDNKQVGTLFLAKNGKGVTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>R5V8M0</accession>
    <name>R5V8M0_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Polyribonucleotide nucleotidyltransferase</fullName>
            <ecNumber evidence="7">2.7.7.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Polynucleotide phosphorylase</fullName>
            <shortName evidence="7">PNPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pnp</name>
        <name evidence="9" type="ORF">BN566_00358</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>phosphate + RNA(n+1) = a ribonucleoside 5'-diphosphate + RNA(n)</text>
            <dbReference type="Rhea" id="RHEA:22096"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the polyribonucleotide nucleotidyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.8" evidence="7"/>
    <dbReference type="EMBL" id="CBAR010000237">
        <property type="protein sequence ID" value="CCZ84459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00358"/>
    <dbReference type="EnsemblBacteria" id="CCZ84459">
        <property type="protein sequence ID" value="CCZ84459"/>
        <property type="gene ID" value="BN566_00358"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004654">
        <property type="term" value="F:polyribonucleotide nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01595">
        <property type="entry name" value="PNPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015847">
        <property type="entry name" value="ExoRNase_PH_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012162">
        <property type="entry name" value="PNPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015848">
        <property type="entry name" value="PNPase_PH_RNA-bd_bac/org-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036456">
        <property type="entry name" value="PNPase_PH_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11252">
        <property type="entry name" value="PTHR11252"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03726">
        <property type="entry name" value="PNPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03725">
        <property type="entry name" value="RNase_PH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005499">
        <property type="entry name" value="PNPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46915">
        <property type="entry name" value="SSF46915"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="SSF54791"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="SSF55666"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03591">
        <property type="entry name" value="polynuc_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="S1 motif" evidence="8">
        <location>
            <begin position="624"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="487"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="493"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ84459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="701" mass="76893" checksum="C3201AB6362F075D" modified="2013-07-24" version="1">MFEKYRKFETTFAGRPLVVETGKTCELSNGSCWVRYGETVVMANVTASAKPREGVDFFPLSVDYEERMYAVGRIPGSFTKREGKPSEKAILTSRMVDRPIRPLFPKDMRNDVSVVMTVLAVDPDCSPEITGMLATSIAISISDIPWNGPIAGISVGLVDGEIVLNPTLEQRAHNDLRLTVAGTMEKIVMIEAGANEIPEQQMLDAILAGHAEIKKMVAFINDIKNEIGKPKFEFQSMELDHDLFAAIEAMVGEQVKVALDTNDKNERDARMQPIIDAVHEKYDPEYPEQGAMLDECLYKLQKKIVRNWLYEGKRVDGRGIDEIRPLAAEVGLLPRVHGSGLFTRGQTQVLTIATLGPLSDSQKIDGLDEETTKRYMHQYNFPSYSVGETKPSRGPGRREIGHGALAEKALVPVLPSVEEFPYAMRLVSEVLSSNGSTSQGSICGSTLALMDAGVPLKAPVAGISCGLITKPDSDEFMTMVDIQGLEDFFGDMDFKVGGTKNGITAIQVDIKVDGLTPAIIAEAFEKTRKARLYILDEIMLKAIPEPRKAVNEYAPKMFQISIPVDKIREVIGQGGKVIQKITAECEVKIDINEDGSVFVSGMKLENCQKALQIINTIAHDPEVGAIYKGKVVSIKPFGAFVEIAPGKDGLVHISKLDNSRVENVEDVVSMGDEIVVKVMEVKDGKISLSRKDALAELQAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>R5V8W3</accession>
    <name>R5V8W3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">30S ribosomal protein S14 type Z</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rpsZ</name>
        <name evidence="7" type="synonym">rpsN</name>
        <name evidence="8" type="ORF">BN566_00070</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Binds 16S rRNA, required for the assembly of 30S particles and may also be responsible for determining the conformation of the 16S rRNA at the A site.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Part of the 30S ribosomal subunit. Contacts proteins S3 and S10.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the universal ribosomal protein uS14 family. Zinc-binding uS14 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000241">
        <property type="protein sequence ID" value="CCZ84524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00070"/>
    <dbReference type="EnsemblBacteria" id="CCZ84524">
        <property type="protein sequence ID" value="CCZ84524"/>
        <property type="gene ID" value="BN566_00070"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01364_B">
        <property type="entry name" value="Ribosomal_S14_2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001209">
        <property type="entry name" value="Ribosomal_S14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043140">
        <property type="entry name" value="Ribosomal_S14/S29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023053">
        <property type="entry name" value="Ribosomal_S14_Z"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19836">
        <property type="entry name" value="PTHR19836"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00253">
        <property type="entry name" value="Ribosomal_S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 7 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 7" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="3 7" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="7">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ84524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="61" mass="7085" checksum="7E0D2DD84D374689" modified="2013-07-24" version="1">MAKKAMKLKQQKTPKFSTRAYTRCKLCGRPHAYLRKYGICRVCFRNLAHEGKIPGVKKASW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>R5V8X5</accession>
    <name>R5V8X5_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">30S ribosomal protein S4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpsD</name>
        <name evidence="10" type="ORF">BN566_00085</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit.</text>
    </comment>
    <comment type="function">
        <text evidence="6">With S5 and S12 plays an important role in translational accuracy.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 30S ribosomal subunit. Contacts protein S5. The interaction surface between S4 and S5 is involved in control of translational fidelity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the universal ribosomal protein uS4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000241">
        <property type="protein sequence ID" value="CCZ84539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00085"/>
    <dbReference type="EnsemblBacteria" id="CCZ84539">
        <property type="protein sequence ID" value="CCZ84539"/>
        <property type="gene ID" value="BN566_00085"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01306_B">
        <property type="entry name" value="Ribosomal_S4_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022801">
        <property type="entry name" value="Ribosomal_S4/S9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001912">
        <property type="entry name" value="Ribosomal_S4/S9_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005709">
        <property type="entry name" value="Ribosomal_S4_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018079">
        <property type="entry name" value="Ribosomal_S4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831">
        <property type="entry name" value="PTHR11831"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831:SF4">
        <property type="entry name" value="PTHR11831:SF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00163">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01390">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01017">
        <property type="entry name" value="rpsD_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00632">
        <property type="entry name" value="RIBOSOMAL_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Ribosomal_S4" evidence="9">
        <location>
            <begin position="3"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="S4" evidence="8">
        <location>
            <begin position="88"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ84539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="198" mass="22584" checksum="3588A07AC330AD8F" modified="2013-07-24" version="1">MAVSREPILKRCRYLGISPMVMGISKESNRNPGANNRKKLSEYGMQLKEKQKLKFIYGVGEKQFYHYFEIATKMEGKAGDNLVTCLESRLDSVVFRMGLALTRREARQLVTHKHFTVNGVRVDIPSYRVKVGDVIALHDTSKSSQKFKDVVEQTADRTVPLWLDVNKEAFSATVTRMPVPEDLDYEVAAHLIVELYSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>R5VGZ4</accession>
    <name>R5VGZ4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tryptophan--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Tryptophanyl-tRNA synthetase</fullName>
            <shortName evidence="8">TrpRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">trpS</name>
        <name evidence="10" type="ORF">BN566_00646</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the attachment of tryptophan to tRNA(Trp).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + H(+) + L-tryptophanyl-tRNA(Trp)</text>
            <dbReference type="Rhea" id="RHEA:24080"/>
            <dbReference type="Rhea" id="RHEA-COMP:9671"/>
            <dbReference type="Rhea" id="RHEA-COMP:9705"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78535"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8 9">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.2" evidence="8"/>
    <dbReference type="EMBL" id="CBAR010000012">
        <property type="protein sequence ID" value="CCZ82841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00646"/>
    <dbReference type="EnsemblBacteria" id="CCZ82841">
        <property type="protein sequence ID" value="CCZ82841"/>
        <property type="gene ID" value="BN566_00646"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004830">
        <property type="term" value="F:tryptophan-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006436">
        <property type="term" value="P:tryptophanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00806">
        <property type="entry name" value="TrpRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00140_B">
        <property type="entry name" value="Trp_tRNA_synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002306">
        <property type="entry name" value="Trp-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024109">
        <property type="entry name" value="Trp-tRNA-ligase_bac-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01039">
        <property type="entry name" value="TRNASYNTHTRP"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00233">
        <property type="entry name" value="trpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="short sequence motif" description="'HIGH' region" evidence="8">
        <location>
            <begin position="15"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="8">
        <location>
            <begin position="198"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="14"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="22"/>
            <end position="23"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>L-tryptophan</name>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="150"/>
            <end position="152"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="198"/>
            <end position="202"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ82841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="334" mass="37492" checksum="12D83501B961F2A3" modified="2013-07-24" version="1">MEEGKKKLIFSGIQPTGTFTLGNYIGAIRNWGPLQDEYRCVYSVVDMHAITVRQDPAKLRQNTLQAYALLLACGIDLEKSILFIQSHVRTHAELSWILGCNTQFGELSRMTQFKDKSAKHADDVNAGLFTYPVLMAADILAYNADLVPVGVDQKQHLELTRNIAQRFNQRYGEFFTLPEPYIPPVGAKVMSLQEPTKKMSKSDENPNACILILDDRDTIIRKFKRAVTDSDSTVRYAEGKDGINNLMTIYSSLTGKDYAAIEQEFAGKGYGDFKMAVGETVADHLQPIQDEFKKLFADKAYLKQCYTDGAAKALAYSQRIVSKVYHKVGFVDAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>R5VHN0</accession>
    <name>R5VHN0_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">Triosephosphate isomerase</fullName>
            <shortName evidence="3">TIM</shortName>
            <shortName evidence="3">TPI</shortName>
            <ecNumber evidence="3 4">5.3.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Triose-phosphate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">tpiA</name>
        <name evidence="5" type="ORF">BN566_00799</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in the gluconeogenesis. Catalyzes stereospecifically the conversion of dihydroxyacetone phosphate (DHAP) to D-glyceraldehyde-3-phosphate (G3P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:18585"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="5.3.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 4">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 4">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the triosephosphate isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.1" evidence="3 4"/>
    <dbReference type="EMBL" id="CBAR010000038">
        <property type="protein sequence ID" value="CCZ83006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00799"/>
    <dbReference type="EnsemblBacteria" id="CCZ83006">
        <property type="protein sequence ID" value="CCZ83006"/>
        <property type="gene ID" value="BN566_00799"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00189"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004807">
        <property type="term" value="F:triose-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00311">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00147_B">
        <property type="entry name" value="TIM_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035990">
        <property type="entry name" value="TIM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022896">
        <property type="entry name" value="TrioseP_Isoase_bac/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000652">
        <property type="entry name" value="Triosephosphate_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020861">
        <property type="entry name" value="Triosephosphate_isomerase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21139">
        <property type="entry name" value="PTHR21139"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00121">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51351">
        <property type="entry name" value="SSF51351"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00419">
        <property type="entry name" value="tim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00171">
        <property type="entry name" value="TIM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51440">
        <property type="entry name" value="TIM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 3 4" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="3 4" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="2 3 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="3">
        <location>
            <begin position="13"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="3">
        <location>
            <begin position="237"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="active site" description="Electrophile" evidence="3">
        <location>
            <position position="98"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="170"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="255" mass="27472" checksum="8DBA43E714593697" modified="2013-07-24" version="1">MNKALRKAIIAGNWKMNKNRKEAAELIEALKPIAADKTCGVIICVPFTNLETALRLTEGTNIEVGAENVHFEEKGAFTGEISADMLTEMGVKYVIVGHSERRQYFGETDETVRKRTTAALKAGMTVILCVGETLEERELGITEEVVAKQVKVALHDACEKCIKNVIIAYEPVWAIGTGKTATAEQAEEVCGFIRKTVAGLFGQAVADGMTIQYGGSMNEKNCAELLAKEDIDGGLIGGASLVAEKFGVLLEEASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5VHY1</accession>
    <name>R5VHY1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative Rrf2 family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00933</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000064">
        <property type="protein sequence ID" value="CCZ83146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83146">
        <property type="protein sequence ID" value="CCZ83146"/>
        <property type="gene ID" value="BN566_00933"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000944">
        <property type="entry name" value="Tscrpt_reg_Rrf2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33221">
        <property type="entry name" value="PTHR33221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02082">
        <property type="entry name" value="Rrf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51197">
        <property type="entry name" value="HTH_RRF2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="140" mass="15374" checksum="C32C390217D4E2B8" modified="2013-07-24" version="1">MQFSSRLTIATHILLCIEIFKDDYKITSNFLAGSIHVNPVIVRNILGLLSSAGIVEIKAGIGGASLAKSPDEITMLDVFKAVEKEEALFHFHENPNANCPVGRNVHAVLDSKLDNIQTAMENELAKITLSQLVKETKEKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5VHY3</accession>
    <name>R5VHY3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ThiF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00938</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000064">
        <property type="protein sequence ID" value="CCZ83151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00938"/>
    <dbReference type="EnsemblBacteria" id="CCZ83151">
        <property type="protein sequence ID" value="CCZ83151"/>
        <property type="gene ID" value="BN566_00938"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008641">
        <property type="term" value="F:ubiquitin-like modifier activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="PTHR10953"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="SSF69572"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ThiF" evidence="1">
        <location>
            <begin position="10"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="253" mass="27167" checksum="2880E75844431FCE" modified="2013-07-24" version="1">MLQPIDSQRYLRQMTLSGFGEAGQAALQAARVLLIGVGGLGCPAALYLAAAGVGTIGLMDGDTVSLLNLHRQILYTDSEIGMPKVTCAANALHRCNSSVTIQTYPVMADADNLPELVRQYDIVLDCTDHLPTKFLINDVCVAERVAYVHGGILAYQGQIFTYVPEETCPCYRCLFEAPPEEGAVPTCKEVGVLGAVAGVIGNLQALEAIRYLTHIGELLTGRLLSFDFATMKTRTVSFPKNPHCIGCGTTERS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VI71</accession>
    <name>R5VI71_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01037</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83256">
        <property type="protein sequence ID" value="CCZ83256"/>
        <property type="gene ID" value="BN566_01037"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="79" mass="9250" checksum="78A8B160A2F199C9" modified="2013-07-24" version="1">MLLKKLVYANQIETFFTFFQFSELFQKLLNTNPFLKKETVQRIAFRQDVPLKSSLFRSFSALQIPFVSSVESDKLLPAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5VIG0</accession>
    <name>R5VIG0_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">WYL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01122</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000092">
        <property type="protein sequence ID" value="CCZ83346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83346">
        <property type="protein sequence ID" value="CCZ83346"/>
        <property type="gene ID" value="BN566_01122"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026881">
        <property type="entry name" value="WYL_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13280">
        <property type="entry name" value="WYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WYL" evidence="1">
        <location>
            <begin position="36"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="223" mass="26637" checksum="835EC0852154900D" modified="2013-07-24" version="1">MVEKLLSYAGVRRSEIVRDNIVFYNNHKHSNQDIYENIEQIELAIRQKKQVSFYYFDLDEKRNRVYRKEKKRYITDPVALVFSEDNYYLVAYSQKYQNAVNYRVDRMDTVEVEEEPICEDALIKKRKTETYTEQVFKMYNGEAETVTLEFAPELLGAVYDKFGEDLEIRHCDGGWLKIKITVQISPPFFGWVFQFKDRMRIVEPSRVADEYIDRIKSAVNDNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5VIQ9</accession>
    <name>R5VIQ9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ABC-type sugar transport system periplasmic component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01195</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000102">
        <property type="protein sequence ID" value="CCZ83421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01195"/>
    <dbReference type="EnsemblBacteria" id="CCZ83421">
        <property type="protein sequence ID" value="CCZ83421"/>
        <property type="gene ID" value="BN566_01195"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008643">
        <property type="term" value="P:carbohydrate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01547">
        <property type="entry name" value="SBP_bac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="418" mass="46014" checksum="BEBA29E3CF6FA42E" modified="2013-07-24" version="1">MDYKSIDLETGDSNLKADLKVITHRTDLIDTTFEEYAQEFQKLYPNVTISFEGITNYADDMTTRLTSGDWGDICMIPTTVNKSEFGTYFEPICNYDDMKDSYEFLGDKMFDGKVYGIASMGNAMGIVYNKSVFEKAGISELPTTPDAFLDDLKQIKDKTDAIPLYTNYAAGWTLTAWDSYIGGTATGDADFMNNTIVHQANPFSKRSDETGPYAVYNTLYEAVSEGLTEDDPTTTDWEGCKSQINNGKIGCMVLGSWAIIQMQEAGDHADDIGYMPFPITVDGKQYASASADYAYGINVNSTADEKKASAAFIKWLTEKSGYAYDQGGIPIVKGETYPETLSAFTNVTLVMDNPTPTGEEDLYANINNGSELSLNSDPTHVARVIESAIDHSETMDEIADSWNEAWTSAQESNGVTPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJ06</accession>
    <name>R5VJ06_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TVP38/TMEM64 family membrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01255</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TVP38/TMEM64 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000117">
        <property type="protein sequence ID" value="CCZ83486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01255"/>
    <dbReference type="EnsemblBacteria" id="CCZ83486">
        <property type="protein sequence ID" value="CCZ83486"/>
        <property type="gene ID" value="BN566_01255"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032816">
        <property type="entry name" value="SNARE_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015414">
        <property type="entry name" value="TMEM64"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12677">
        <property type="entry name" value="PTHR12677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09335">
        <property type="entry name" value="SNARE_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="25"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="99"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="215"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU366058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="245" mass="27165" checksum="486E8A8A8CBEEDA6" modified="2013-07-24" version="1">MKEKETTNAMSCPTKSPEASARWKMILSGVIFLVLVVAVTILMIPVMRLMMQEGGKEQLIAKIQSYGIFAPLLFVLLQVIQIVVAFIPGGPIPMIGGAVFGMVPGILLSLAGSFLGTAIVYYLVQWIGRPLLNLFVKEEHLERFSFLTNRRKMERIVFLLFLCPGLPKDALTYIVAFNKTIPPLPLFFLTTIARFPAMALTVKMGSSLWEGNWKMTALVVGILILIAVAGGLIRWHHKKKHGKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5VKP0</accession>
    <name>R5VKP0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">PF08719 domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01802</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + H2O = 2,5,6-triamino-4-hydroxypyrimidine + D-ribose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:23436"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58614"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:137796"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-amino-6-(5-phospho-D-ribosylamino)uracil + H2O = 5,6-diaminouracil + D-ribose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:55020"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:46252"/>
            <dbReference type="ChEBI" id="CHEBI:58453"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000186">
        <property type="protein sequence ID" value="CCZ84076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01802"/>
    <dbReference type="EnsemblBacteria" id="CCZ84076">
        <property type="protein sequence ID" value="CCZ84076"/>
        <property type="gene ID" value="BN566_01802"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd15457">
        <property type="entry name" value="NADAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012816">
        <property type="entry name" value="NADAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037238">
        <property type="entry name" value="YbiA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08719">
        <property type="entry name" value="NADAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143990">
        <property type="entry name" value="SSF143990"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02464">
        <property type="entry name" value="ribofla_fusion"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADAR" evidence="3">
        <location>
            <begin position="7"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="164" mass="18740" checksum="021001C7C844B5F5" modified="2013-07-24" version="1">MKTVIGFHRPEEINGSFSNWYLSDFVVDGIKYSSMEQYMMHKKALLFKDTEIASQILCTSNPGEIKNLGRCVSNYNNSVWNGVRQLIVYKGLLEKFRQNDDLSQKLLETGEAILAECAVHDLIWGIGISMTDPARFNIHAWKGQNLLGYALMMVREELHHTPSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>R5VKQ1</accession>
    <name>R5VKQ1_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">DNA repair protein RecO</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6 7">Recombination protein O</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">recO</name>
        <name evidence="9" type="ORF">BN566_01817</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in DNA repair and RecF pathway recombination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the RecO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000186">
        <property type="protein sequence ID" value="CCZ84091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01817"/>
    <dbReference type="EnsemblBacteria" id="CCZ84091">
        <property type="protein sequence ID" value="CCZ84091"/>
        <property type="gene ID" value="BN566_01817"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00201">
        <property type="entry name" value="RecO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022572">
        <property type="entry name" value="DNA_rep/recomb_RecO_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003717">
        <property type="entry name" value="RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042242">
        <property type="entry name" value="RecO_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33991">
        <property type="entry name" value="PTHR33991"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02565">
        <property type="entry name" value="RecO_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11967">
        <property type="entry name" value="RecO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="SSF57863"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00613">
        <property type="entry name" value="reco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4 7" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="5 7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA_rep/recomb_RecO_N" evidence="8">
        <location>
            <begin position="2"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00033409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF11967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ84091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="262" mass="30221" checksum="75E8B5F847B53C0E" modified="2013-07-24" version="1">MLTKRCLILQERQAGDAGRFLDILTKTEGVLEVFVRGSKKANSKHLAATQPFAYSKLCLAEKNGRYYVESAEPIHLFYGLREHLTQLALAAYFFDLIRYAVPTGQQQNFILRLTLNTLYYLTTGNRSEAFLKSVFELRFLSELGMMPDVLVCPVCMEYLPQSLFYSARTGHFYCQKCYTPKEEDYAVPMSLGGLQAIRHIVLTEPERLFQFRLGAETQQAVNQFSETFVRYQLDYQPQTLQFYKRLLSTQPPLPRPSSTTDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>R5VLY3</accession>
    <name>R5VLY3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">50S ribosomal protein L14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rplN</name>
        <name evidence="6" type="ORF">BN566_00067</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 5">Binds to 23S rRNA. Forms part of two intersubunit bridges in the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Part of the 50S ribosomal subunit. Forms a cluster with proteins L3 and L19. In the 70S ribosome, L14 and L19 interact and together make contacts with the 16S rRNA in bridges B5 and B8.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the universal ribosomal protein uL14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000241">
        <property type="protein sequence ID" value="CCZ84521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00067"/>
    <dbReference type="EnsemblBacteria" id="CCZ84521">
        <property type="protein sequence ID" value="CCZ84521"/>
        <property type="gene ID" value="BN566_00067"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.150.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01367">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036853">
        <property type="entry name" value="Ribosomal_L14_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000218">
        <property type="entry name" value="Ribosomal_L14P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005745">
        <property type="entry name" value="Ribosomal_L14P_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019972">
        <property type="entry name" value="Ribosomal_L14P_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761">
        <property type="entry name" value="PTHR11761"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00238">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01374">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50193">
        <property type="entry name" value="SSF50193"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01067">
        <property type="entry name" value="rplN_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00049">
        <property type="entry name" value="RIBOSOMAL_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3 4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 5" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ84521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="122" mass="13462" checksum="E8D2327D58C35F0B" modified="2013-07-24" version="1">MIQMQTYLKVADNTGAKELMCIRVLGGTRRRYANIGDVVVASVKKATPGGVVKKGDVVKAVIVRSAKGLRREDGTYIRFDENAAVIIKEDKNPKGTRIFGPVARELRDKDYMKILSLAPEVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5VM98</accession>
    <name>R5VM98_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">30S ribosomal protein S16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rpsP</name>
        <name evidence="4" type="ORF">BN566_00524</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the bacterial ribosomal protein bS16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000267">
        <property type="protein sequence ID" value="CCZ84671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00524"/>
    <dbReference type="EnsemblBacteria" id="CCZ84671">
        <property type="protein sequence ID" value="CCZ84671"/>
        <property type="gene ID" value="BN566_00524"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00385">
        <property type="entry name" value="Ribosomal_S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000307">
        <property type="entry name" value="Ribosomal_S16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023803">
        <property type="entry name" value="Ribosomal_S16_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12919">
        <property type="entry name" value="PTHR12919"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00886">
        <property type="entry name" value="Ribosomal_S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54565">
        <property type="entry name" value="SSF54565"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00002">
        <property type="entry name" value="S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="80" mass="8990" checksum="8277E4FF918745BE" modified="2013-07-24" version="1">MAVKIRLRRMGAKKAPFYRIVVADERYPRDGRFIEEIGSYDPTKNPAAVVVDADKVKQWLGNGAQPTDSVKCILKKEGIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5VQC3</accession>
    <name>R5VQC3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alcohol dehydrogenase class IV</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00587</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000008">
        <property type="protein sequence ID" value="CCZ82782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00587"/>
    <dbReference type="EnsemblBacteria" id="CCZ82782">
        <property type="protein sequence ID" value="CCZ82782"/>
        <property type="gene ID" value="BN566_00587"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd08179">
        <property type="entry name" value="NADPH_BDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001670">
        <property type="entry name" value="ADH_Fe/GldA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018211">
        <property type="entry name" value="ADH_Fe_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039697">
        <property type="entry name" value="Alcohol_dehydrogenase_Fe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034802">
        <property type="entry name" value="NADPH_BDH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11496">
        <property type="entry name" value="PTHR11496"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00465">
        <property type="entry name" value="Fe-ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00913">
        <property type="entry name" value="ADH_IRON_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe-ADH" evidence="2">
        <location>
            <begin position="7"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ82782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="403" mass="43795" checksum="F1526E0BF373EE43" modified="2013-07-24" version="1">MSRFTIPRDLYHGKGSLEELKNLQGKKAMVVVGGGSMKRFGFLDQAVAYLKEAGMEVALIEGVEPDPSVTTVLKGAEKMREFEPDWIVAMGGGSPIDAAKAMWAFYEYPDTKFEDLCTPFNFPTLRTKAKFCAIPSTSGTATEVTAFSVITDYDKGIKYPLADFNITPDIAIVDSALAETMPKKLVAHTGMDAMTHAIEAYVSTLHCNYTDAVALHAIKMIHKELKKSFDGCMTARAEMHDAQCLAGMAFSNALLGIVHSMAHKTGAAYTGDHIIHGCANAMYLPKVIKYNAKNEEAAERYAQIADHIGLGGTTTAEKVDALIAELRSMNDQLDIPQAIKFYGAGGVKAETSIIDEAEFLEKLPTVAANAIGDACTGSNPRQPSQEEMEKLLKACYYDLEIDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5VQI7</accession>
    <name>R5VQI7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00652</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000012">
        <property type="protein sequence ID" value="CCZ82847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82847">
        <property type="protein sequence ID" value="CCZ82847"/>
        <property type="gene ID" value="BN566_00652"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="176" mass="20316" checksum="99BA3ABB84766A31" modified="2013-07-24" version="1">MINNIHYEEALNNILIVVIAAAVVLVLVMLLGSKKSRFRKKLSAFFADDTVGTAKHPMEFNREYYITGEKNMRKPAKTKGATRTEMVMLVQKVDERRRTIDPNGTSKLYNEYYELIFKTRKGEILHIVTTKVVYKEVPFNQQGSLTFRGEQFVKFKYLGGEITEAKTHYNAEVINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>R5VQL8</accession>
    <name>R5VQL8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Precorrin-6Y C5 15-methyltransferase (Decarboxylating) CbiT subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_00674</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000020">
        <property type="protein sequence ID" value="CCZ82872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00674"/>
    <dbReference type="EnsemblBacteria" id="CCZ82872">
        <property type="protein sequence ID" value="CCZ82872"/>
        <property type="gene ID" value="BN566_00674"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd11644">
        <property type="entry name" value="Precorrin-6Y-MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012818">
        <property type="entry name" value="CbiE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014008">
        <property type="entry name" value="Cbl_synth_MTase_CbiT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="SSF53790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02467">
        <property type="entry name" value="CbiE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02469">
        <property type="entry name" value="CbiT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TP_methylase" evidence="3">
        <location>
            <begin position="3"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="Methyltranfer_dom" evidence="4">
        <location>
            <begin position="250"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ82872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="402" mass="43635" checksum="37D3697FAB1D5521" modified="2013-07-24" version="1">MHKLWLIGGGSGQPDYLLPLAKQVLGQADCVIASERFLKLAAGKKQIPMKQLSVLLEQLPTLLQTDSVAILVSGDPLLYSFYRTVRNRYPDWDITVIPGIGSLQLLGAKFGLTMEDAFISSLHGKPYTAGSIACAVAQHALTFFFCSAKDGVRQIAQALCQYQLSDTTMYIGADLTYETEQTWSGAPEQFCSAENPALCVTAVRNPNPKPIGAAVFLPDDAFLRNGAPMTKEEVRAVIISKLRLQPDSIVWDLGAGTGSVSITCAKCCPYGQVYAVEYQEQALDILRQNCAYLQAENVTVIAGRAETVLSELPVPDCIFIGGSNGAFPEILRQIQQLPKPVRLVVSAVTLETQAEVTQLLQDAPQLEIIPVFSGQSRKVGRYHVLQPYHPVLLFACTGGKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5VRA4</accession>
    <name>R5VRA4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">1-acyl-sn-glycerol-3-phosphate acyltransferases</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00870</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000051">
        <property type="protein sequence ID" value="CCZ83082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00870"/>
    <dbReference type="EnsemblBacteria" id="CCZ83082">
        <property type="protein sequence ID" value="CCZ83082"/>
        <property type="gene ID" value="BN566_00870"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="PlsC" evidence="3">
        <location>
            <begin position="42"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="208"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="251" mass="28931" checksum="F57F3332A3AA02EB" modified="2013-07-24" version="1">MYNHIVAFFYRLAYYIVRFAFFFYYDIQFEGRENAPPKTESAIFASNHRSYLDPVLVAMGAPHPFNYIAKEELFKHKVFARFIWFMGAFPATWKADPNYDMLSEAVKRLDNNRHLTIFPEGTRHTDGKVGSGKSGVCVLAAKSGKPVIPVGLIFNSNNLHFRSKICVRLGKPIYASDYGLTPESTPREMRQMKQDIMQAIRELVEENPPFPIKHDQPHKQSAVDRHRAERLETIHHQEEAAAEQAAKEQQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>R5VRH2</accession>
    <name>R5VRH2_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 7">Pyruvate, phosphate dikinase</fullName>
            <ecNumber evidence="4 7">2.7.9.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN566_00118</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>ATP + phosphate + pyruvate = AMP + diphosphate + H(+) + phosphoenolpyruvate</text>
            <dbReference type="Rhea" id="RHEA:10756"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.9.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 7 10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the PEP-utilizing enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.9.1" evidence="4 7"/>
    <dbReference type="EMBL" id="CBAR010000068">
        <property type="protein sequence ID" value="CCZ83157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00118"/>
    <dbReference type="EnsemblBacteria" id="CCZ83157">
        <property type="protein sequence ID" value="CCZ83157"/>
        <property type="gene ID" value="BN566_00118"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050242">
        <property type="term" value="F:pyruvate, phosphate dikinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006090">
        <property type="term" value="P:pyruvate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008279">
        <property type="entry name" value="PEP-util_enz_mobile_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018274">
        <property type="entry name" value="PEP_util_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000121">
        <property type="entry name" value="PEP_util_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036637">
        <property type="entry name" value="Phosphohistidine_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002192">
        <property type="entry name" value="PPDK_AMP/ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010121">
        <property type="entry name" value="Pyruvate_phosphate_dikinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_Kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22931">
        <property type="entry name" value="PTHR22931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00391">
        <property type="entry name" value="PEP-utilizers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02896">
        <property type="entry name" value="PEP-utilizers_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01326">
        <property type="entry name" value="PPDK_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000853">
        <property type="entry name" value="PPDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="SSF51621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52009">
        <property type="entry name" value="SSF52009"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01828">
        <property type="entry name" value="pyru_phos_dikin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00370">
        <property type="entry name" value="PEP_ENZYMES_PHOS_SITE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PPDK_N" evidence="12">
        <location>
            <begin position="18"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="PPDK_N" evidence="12">
        <location>
            <begin position="306"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="PEP-utilizers" evidence="11">
        <location>
            <begin position="425"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="PEP-utilizers_C" evidence="13">
        <location>
            <begin position="523"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="8">
        <location>
            <position position="458"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="835"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="564"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="620"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9 10">
        <location>
            <position position="749"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="770"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="771"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="772"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9 10">
        <location>
            <position position="773"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000853-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000853-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000853-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CCZ83157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="878" mass="96700" checksum="2DFF0F1A4A64D6AE" modified="2013-07-24" version="1">MANKWVYMFSEGNMTMRNLLGGKGANLAEMTTIGLPVPQGFTITTEACTQYYEDGRKINDEIMTQVMDGVKKMEEINGKKFGDKKNPLLVSVRSGARASMPGMMDTILNLGLNDDVVAAMIEGNPDPAFERFVYDSYRRFIQMFSDVVMEVGKKYFEQLIDKMKEEKGVKFDVELTAADLKTLAEQFKAEYKKQLGSDFPSDPVEQLKLAIEAVFRSWDNPRANVYRRDNDIPYSWGTAVNVMPMVFGNLNNESGTGVAFTRDPATGEKKLMGEFLINAQGEDVVAGVRTPMPIAQMEQEFPEAYAEFIQVCKTLEDHYHDMQDMEFTVENKKLYMLQCRNGKRTAQAALQIACDLVDEKHKTEEEAVLMIDPRNLDTLLHPQFDAKALKAATPIGKGLGASPGAACGKVVFTADDAEAWNAKGEKVVLVRLETSPEDITGMKASQGILTVRGGMTSHAAVVARGMGTCCVSGCGDIKMDEENKKFELAGKTFTEGDYISIDGSTGNIYDGIIPTVDASITGTFGRVMGWADKYRTMKVRTNADTPADAKKARELGAEGIGLCRTEHMFFEADRIAAFREMICSDTVEEREAALAKIEPMQQGDFEALYEALEGNPVTIRFLDPPLHEFVPTEEEDIKKLADAQGKTVDQIKAIIASLHEFNPMMGHRGCRLAVTYPEIAKMQTKAIIKAAINVKAKHPDWTVKPEIMIPLVGDVKELKFVKKVVVETADAEIAAAGSDLTYEVGTMIEIPRAALTADEIAKEADFFCFGTNDLTQMTYGFSRDDAGKFLGAYYDAKIFESDPFAKLDQTGVGKLMKMAIELGKPVNSKLHCGICGEHGGDPTSVEFCHEIGLDYVSCSPFRVPIARLAAAQAAIKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5VRV4</accession>
    <name>R5VRV4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Listeria/Bacterioides repeat</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01073</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01073"/>
    <dbReference type="EnsemblBacteria" id="CCZ83292">
        <property type="protein sequence ID" value="CCZ83292"/>
        <property type="gene ID" value="BN566_01073"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041219">
        <property type="entry name" value="Phage_lysozyme2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18013">
        <property type="entry name" value="Phage_lysozyme2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004407335" evidence="1">
        <location>
            <begin position="28"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="Phage_lysozyme2" evidence="2">
        <location>
            <begin position="33"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="414" mass="46099" checksum="357CDEA50E725C07" modified="2013-07-24" version="1">MKSKKKWIAAMCSILLSSSMLTPAAFAVEVQSNEEMIYTFLTEELQLNAAVASGILANLYQESGFEPTASCIDSNHQISYGICQWNGARYADLQAFCSENGYAYDSLDGQLAYLENDLLTTYAPYYYDTLLNDFENSEDGAYEAAYSWAAIYEVCSTLYRSGRAELARDVFFPSYESYLPPEESPSDVGSDFYTTLQVGDDSKTLSMTAEKQVQFQTESTPTQWHVSKQDDGSYFLMQSEGRYYLNGQTDLLTVSEEPSRWWIYQTENGYVLQDAETEQVLQDSLALQDTDIETGTAFSMHPLQAPATAPLTVKTDTTQAIFQWTAAADTDSYSLTIWNGTDSTQTPLQNDNCLQGNSFRTTLPAGTYTAVLKQINAAGTTTGEPVTFTITEPILKTKRSKADGFHFDKDKQLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5VT51</accession>
    <name>R5VT51_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">IMP_cyclohyd domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01448</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000154">
        <property type="protein sequence ID" value="CCZ83712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01448"/>
    <dbReference type="EnsemblBacteria" id="CCZ83712">
        <property type="protein sequence ID" value="CCZ83712"/>
        <property type="gene ID" value="BN566_01448"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003937">
        <property type="term" value="F:IMP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006188">
        <property type="term" value="P:IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020600">
        <property type="entry name" value="IMP_cyclohydrolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036795">
        <property type="entry name" value="IMP_cyclohydrolase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07826">
        <property type="entry name" value="IMP_cyclohyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75569">
        <property type="entry name" value="SSF75569"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IMP_cyclohyd" evidence="1">
        <location>
            <begin position="15"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="236" mass="26067" checksum="C61E2DBED6507A3C" modified="2013-07-24" version="1">MKTNNLFDTLKQNAYPGRGIVLGTSADGASAVAAYFIMGRSENSRNRVFVTDGDGIRTQAFDPSKMVDPSLIIYAPVRVLGDTTIVTNGDQTDTVYDQMLAGKTFEESLRIREFEPDAPNYTPRISGIIERKDGYQYALSILKSADGNPDSCQRYTFTYSNPIAGVGHFIHTYQGDGNPLPSFEGEPEKVAIEGDIDTFTNAVWDSLNPENKVSLFVRFIDLKTGKAETRIVNKNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5VTB5</accession>
    <name>R5VTB5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01517</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000161">
        <property type="protein sequence ID" value="CCZ83782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83782">
        <property type="protein sequence ID" value="CCZ83782"/>
        <property type="gene ID" value="BN566_01517"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023804">
        <property type="entry name" value="DUF3792_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12670">
        <property type="entry name" value="DUF3792"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04086">
        <property type="entry name" value="TIGR04086_membr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="29"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="91"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="126"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="150" mass="15915" checksum="A7836F4AD6EF8915" modified="2013-07-24" version="1">MHPKRCSSCPPPPPCKKRPVRRTCWQSPVWSTVLAVLAGLAVVLLLLLLFSLLISNIDASEELVAAMGCVSLCAGSFTAGFVAAKRRRCKGLLIGLACGIAMYLVTFLIGLLLLQSFAAAGTFSKLIFMVLCSCIGGVVGVNTKCRVPRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VTI3</accession>
    <name>R5VTI3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01572</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000166">
        <property type="protein sequence ID" value="CCZ83837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83837">
        <property type="protein sequence ID" value="CCZ83837"/>
        <property type="gene ID" value="BN566_01572"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="269" mass="30998" checksum="03ED9F397A54FBF3" modified="2013-07-24" version="1">MKHMQSMALAIVRCYSLFKKSRGMRLLGGISCAVLCSSLIGCLPTVAETVEINGKSYRTGFYGDCYLMDDLPLSGVEYELDEITYRALQHPKFDFWYNGADSVMYCAEDQWEAAKTYYADDQQYFYYCSVGVTIKDQTEPTLYDISGKVDTQKFKELLEFGEGNEYIPFAYGKNSEQERKTVTMPLPDNKQRLIFYRESKDGCFCSYRGSHFYVVDGQLYLTYQYQMGRKEWIATPVPEALSNYFCELVKDFDEINKETATNEGKSTKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5VTR5</accession>
    <name>R5VTR5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Hydrid cluster protein-associated redox disulfide domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01641</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000172">
        <property type="protein sequence ID" value="CCZ83912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01641"/>
    <dbReference type="EnsemblBacteria" id="CCZ83912">
        <property type="protein sequence ID" value="CCZ83912"/>
        <property type="gene ID" value="BN566_01641"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3910.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023883">
        <property type="entry name" value="CHP03980_redox-disulphide"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015077">
        <property type="entry name" value="DUF1858"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038062">
        <property type="entry name" value="ScdA-like_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39341">
        <property type="entry name" value="PTHR39341"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08984">
        <property type="entry name" value="DUF1858"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140683">
        <property type="entry name" value="SSF140683"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03980">
        <property type="entry name" value="prismane_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1858" evidence="1">
        <location>
            <begin position="5"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="76" mass="8004" checksum="5D5BA83521BC9BE3" modified="2013-07-24" version="1">MAVTITKDTIIGDILDIAPETAPLFMAIGMHCLGCPASRGETVEEACMVHGIEVEELLEELNKQIASSTAATTTEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>R5VTU0</accession>
    <name>R5VTU0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Asparagine synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN566_01666</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the asparagine synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000173">
        <property type="protein sequence ID" value="CCZ83937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01666"/>
    <dbReference type="EnsemblBacteria" id="CCZ83937">
        <property type="protein sequence ID" value="CCZ83937"/>
        <property type="gene ID" value="BN566_01666"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004066">
        <property type="term" value="F:asparagine synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006529">
        <property type="term" value="P:asparagine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01991">
        <property type="entry name" value="Asn_Synthase_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00712">
        <property type="entry name" value="AsnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006426">
        <property type="entry name" value="Asn_synth_AEB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001962">
        <property type="entry name" value="Asn_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033738">
        <property type="entry name" value="AsnB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00733">
        <property type="entry name" value="Asn_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001589">
        <property type="entry name" value="Asn_synthetase_glu-h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01536">
        <property type="entry name" value="asn_synth_AEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0061">Asparagine biosynthesis</keyword>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 5" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="8">
        <location>
            <begin position="2"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="5">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="376"/>
            <end position="377"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for beta-aspartyl-AMP intermediate formation" evidence="7">
        <location>
            <position position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ83937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="611" mass="69885" checksum="DB27928C257981D0" modified="2013-07-24" version="1">MCGITGMVTWQEDVARYQADLKRMQAVLSNRGPDQQGIYQDTHCAMAHTRLAVIDVERGKQPMTRQTATETYTLVYNGELYNTPELREELQKEGAVFTTHCDTEVVLQAYLHWGAACTERFNGIFAFAVWEQQAQRLFLARDRIGVKPLFYAEIPGGLVFASEIKALLQHPAVPHTIDKHGIAQILLLGPGRSAGCGVFQTIKEVKRAHCGYATETGISLAPYWKLQAKPHTDNMEQTIEQVRELVKDAATRQMVSDVPIGTFLSGGLDSSLLSSLANQVLKKRGEVLHTFSVDYKDNDRYFQKSHFQPNSDPDYIRAMMNYLQCQHHWTVLDTPQLAAALIPAMQARDLPGMVDVDSSLYLFCGAIKEQVTVALSGECADELFGGYPWYRDPNIRERDGFPWAQSTDYRMQFLREEFAEQIDGAAFVSQAYQETIQQADALPEDSPLERRMRQMMLLNLDWFMQTLLDRKDRMSMANALEVRVPFCDYRIAEYLYNVPWEMKEYKGYEKGLLRAAMQDYLPEKVLWRKKSPYPKTHNPNYLQAVRAMLAAILQDPKAPLFQLVKREAIEQLLLQPSGVQWYGQLMAYPQTIAYFVQMNAWLQAYDVKIVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VU88</accession>
    <name>R5VU88_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01803</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000186">
        <property type="protein sequence ID" value="CCZ84077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84077">
        <property type="protein sequence ID" value="CCZ84077"/>
        <property type="gene ID" value="BN566_01803"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="201" mass="22987" checksum="EEB9930CE4C7BD10" modified="2013-07-24" version="1">MNVLIENGKIICKRKIFSKEIFPTELEKIIVTNSGTVLQFKNHSKPIAAKNKLNPLLDDLSCLIKNQISYEDTTQETCGYPENQISELLHRTKSHAETIANKILSNHLGKEYAVHIEIVGEPLYSMLVFRLKKEEQVLYQHPSYEKIEKLGVDTALDAMDLTFLIKWDASCKQGLYGITQEVENQKELEAYIVSLIEDKFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>R5VV02</accession>
    <name>R5VV02_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN566_00260</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CBAR010000217">
        <property type="protein sequence ID" value="CCZ84347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00260"/>
    <dbReference type="EnsemblBacteria" id="CCZ84347">
        <property type="protein sequence ID" value="CCZ84347"/>
        <property type="gene ID" value="BN566_00260"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="10">
        <location>
            <begin position="189"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="9">
        <location>
            <begin position="256"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="226"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ84347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="478" mass="54138" checksum="0B1A7DCF82C1AF7A" modified="2013-07-24" version="1">MTIKKRLFLSNLFMLLIPAMVSVLILAVSLLFFVNVVYKQVMDETVREENTVHLEHLLMEQAKEFLQTKHEVLTDSHLYQTVEKYVGTQEIQLQIYQGDAQICSMGTSTQALASQSLQELSSSTPNGSLYMEQAEIEGISYTIYLFFSAPVKESAWNELIVKYWILVLFVLIVAAVLVTNQFLTKFVFRKIEKPLDLLVSGVHEIQNGNLTYRIVYKEKDEFLPVFKDFNQMAERLQQSIEQIEREERNRKELIAGISHDLRTPLTSIKAYIEGLIEGIAQTPERQQKYMQTIREKANDIDRMVDKLFLFSKLDLGDSPFYPEPISVAEALNSLISENAEAYAKQGMTITLEEIPEACTVYADPVQFRNAITNLLENSLKYKDAPEVQVRIRCTETDSDATIIVSDNGPGVPEEALPKLFDVFYRSDPARSNPQSGSGLGLAITAKILERFGGSIRAEQETPKGLRILLTIPKKGVET</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVA5</accession>
    <name>R5VVA5_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein-export membrane protein SecF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">secF</name>
        <name evidence="11" type="ORF">BN566_00344</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. SecDF uses the proton motive force (PMF) to complete protein translocation after the ATP-dependent function of SecA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Forms a complex with SecD. Part of the essential Sec protein translocation apparatus which comprises SecA, SecYEG and auxiliary proteins SecDF. Other proteins may also be involved.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the SecD/SecF family. SecF subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000225">
        <property type="protein sequence ID" value="CCZ84437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00344"/>
    <dbReference type="EnsemblBacteria" id="CCZ84437">
        <property type="protein sequence ID" value="CCZ84437"/>
        <property type="gene ID" value="BN566_00344"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015450">
        <property type="term" value="F:protein-transporting ATPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043952">
        <property type="term" value="P:protein transport by the Sec complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01464_B">
        <property type="entry name" value="SecF_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022813">
        <property type="entry name" value="SecD/SecF_arch_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022645">
        <property type="entry name" value="SecD/SecF_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005665">
        <property type="entry name" value="SecF_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30081">
        <property type="entry name" value="PTHR30081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02355">
        <property type="entry name" value="SecD_SecF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01755">
        <property type="entry name" value="SECFTRNLCASE"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="179"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="207"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="261"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="285"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="10">
        <location>
            <begin position="145"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ84437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="332" mass="36348" checksum="A826691FB9B344E9" modified="2013-07-24" version="1">MAEKEKQFKHYPVDVKVRNFCGKSKLFFLLTLILVVIAIASSFTGVDIALEFKGGTIITYSYEGEAVDKSAISSELTDLIGTSVNVQDGESLDGGSKTLTLSFTSNEGLTVDRQAEVTDAIQSRYPDANVQLLDSNDVSARSGSDFFAKCIVAAIFAAVILILYIAFRFKQISGWSAGVCAIIGLLATLVITYGSVVLMGFEIDSNFMAVILTLLGYAVNDTIVIYDRIRENQEKMPGMYIEDLVNTSSSQSLRRSIRTSLTTLFAMVAVVVVSAIMGLDSIQHFAIPMAVGILVGTYHSICFVPSLWVWWQKRRGVETLKVIPKKKKKATE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVB8</accession>
    <name>R5VVB8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase_C39_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00046</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000230">
        <property type="protein sequence ID" value="CCZ84447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84447">
        <property type="protein sequence ID" value="CCZ84447"/>
        <property type="gene ID" value="BN566_00046"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039564">
        <property type="entry name" value="Peptidase_C39-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13529">
        <property type="entry name" value="Peptidase_C39_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004407404" description="Peptidase_C39_2 domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_C39_2" evidence="2">
        <location>
            <begin position="77"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="266" mass="29760" checksum="7FE0C7E57B4C66F8" modified="2013-07-24" version="1">MKHTIGLMLSGLAATILFCACTASPESVAVKISQDESEELSILAETTVTMTQQTIQTTTTTERKPDLVEEVPAEHVLDVDVIYQRPELPTGCEITSLTALLQYLGFDVDKTTMAEDFLPCSYDTAKNTLDDAYIGLPQASDGFGCYAPVLVKTADAYLKTQDSEKQGLDLTGSSEETICRYIASGYPVVMWITIGLYDVYEEEGWTTEDGRTVMWCDLEHCVLLHGYDKENDLVYVCDPLNGDVTYSMERYFQIYEDMHKRAMTIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVG1</accession>
    <name>R5VVG1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative enoyl-(Acyl-carrier-protein) reductase II</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00386</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000240">
        <property type="protein sequence ID" value="CCZ84487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00386"/>
    <dbReference type="EnsemblBacteria" id="CCZ84487">
        <property type="protein sequence ID" value="CCZ84487"/>
        <property type="gene ID" value="BN566_00386"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018580">
        <property type="term" value="F:nitronate monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04730">
        <property type="entry name" value="NPD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017569">
        <property type="entry name" value="Enoyl_ACP_red-II_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004136">
        <property type="entry name" value="NMO"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03060">
        <property type="entry name" value="NMO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03151">
        <property type="entry name" value="enACPred_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NMO" evidence="1">
        <location>
            <begin position="3"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="NMO" evidence="1">
        <location>
            <begin position="84"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="308" mass="32361" checksum="892B96CD966F5EA5" modified="2013-07-24" version="1">MKTKITELFGCKYPIFQGGMAWIAESTLASAVTNAGGVGIIAGGSAPIDYLREQICICKEKVGDKPFGVNIMLMSPNAEDLAQLVIDEKVPMITTGAGNPGKYMAAWKEAGIKVVPVVPSVALARRMERSGADAIIAEGMESGGHIGQNTTMCLVPQVVDAVSVPVIAAGGIADGRGIAAAFMLGAEGVQMGTRFLATTECQIHENYKNLVLKAKDTDNAVTGVFSGSPCRGVKTKFTKKLLELEKKAAPPEDFEALTVGSLRKAVVDGNVEEGSFLCGLIAGMVHDVKPCAEVIQEMMGQAETLLNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVG7</accession>
    <name>R5VVG7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00391</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000240">
        <property type="protein sequence ID" value="CCZ84492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00391"/>
    <dbReference type="EnsemblBacteria" id="CCZ84492">
        <property type="protein sequence ID" value="CCZ84492"/>
        <property type="gene ID" value="BN566_00391"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013114">
        <property type="entry name" value="FabA_FabZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30272">
        <property type="entry name" value="PTHR30272"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07977">
        <property type="entry name" value="FabA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="145" mass="16030" checksum="B6165EC518E0F675" modified="2013-07-24" version="1">MAEVLLNQEQIKEIIPHRDPFLLIDEVLSMEVGKKVVARKYIKEDDFWFKGHFPEKPVTPGVLMIEMLAQAGAVCILSQPEFKGKIALFAGIDKAKFRKQVVPGDVLDLEVEIISQRGPIGIGKAVASVDGKKAVTCEIKFAVEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVM2</accession>
    <name>R5VVM2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Scaffoldin C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_00088</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000241">
        <property type="protein sequence ID" value="CCZ84542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84542">
        <property type="protein sequence ID" value="CCZ84542"/>
        <property type="gene ID" value="BN566_00088"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008965">
        <property type="entry name" value="CBM2/CBM3_carb-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002102">
        <property type="entry name" value="Cohesin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00963">
        <property type="entry name" value="Cohesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49384">
        <property type="entry name" value="SSF49384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004395760" evidence="4">
        <location>
            <begin position="28"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="5">
        <location>
            <begin position="230"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="207"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="207"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ84542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="304" mass="31434" checksum="553CA088AC4BC3EE" modified="2013-07-24" version="1">MKTKRTMAGILAMAMAASALAFNPVFAAGEIGISAEKVSAKAGAEFTLAVSLKDVPSTGVGGCEFSLAYDSSIVTVTGVTAGTIAQTGADDQESAFAGEAPSFDSNFSAAGVIDVIYSTGLTDSAYMVKQDGVFLTITGTVNESAKVGDVSDFKIQPIDRETTPGSGTKNDQIVFASIAEDGTVSSYDTVVSDGSVTVVGDSETTTAVTTTTGSDSTTTETKETTTESESDINYGDANLDGKVDLTDAITLNKYLAGVLTASDQAVKNMNVYLDSNVDDKDTTYLMKFILNVDGYQDLPITPEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5UWQ7</accession>
    <name>R5UWQ7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Dockerin type I repeat protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00563</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000003">
        <property type="protein sequence ID" value="CCZ82758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00563"/>
    <dbReference type="EnsemblBacteria" id="CCZ82758">
        <property type="protein sequence ID" value="CCZ82758"/>
        <property type="gene ID" value="BN566_00563"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1380.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003343">
        <property type="entry name" value="Big_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009045">
        <property type="entry name" value="Hedgehog_sig/DD-Pept_Zn-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008964">
        <property type="entry name" value="Invasin/intimin_cell_adhesion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003709">
        <property type="entry name" value="Pept_M15B"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02368">
        <property type="entry name" value="Big_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02557">
        <property type="entry name" value="VanY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00635">
        <property type="entry name" value="BID_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49373">
        <property type="entry name" value="SSF49373"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55166">
        <property type="entry name" value="SSF55166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="BIG2" evidence="3">
        <location>
            <begin position="147"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ82758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="426" mass="46554" checksum="BEED21344364AFDC" modified="2013-07-24" version="1">MISHAATADDAWRPVVQPNRPQQSAASEEPLPRLQKRPVVSHAMPEQEDRDLEESWPDVDTLEEIPDEPPQEERRRLPKGRKTLAIIIAVYALILVAGFAARFIINAVQKNKPEDASSSVSSSSQVDDSSTPDVTAAETTEKAAAGDAEIVSITPDETYLAVLEDGTATIKVSLSAHGAANGSSLQWTSSDEKIATVDENGAVKGVAAGTCTISVSAKNDPSVSADVDVAVRHLEEKDGCTYVDGILIVNKTYSLPESYDPAGLTDETQTALDKMIEDAYAAGLNLYDASDYRSYSDQISVYQEYCARDGWEQADTYSARPGHSEHQTGMVIDFNDINSDFDDTAEAQWLAENCANYGFIVRFPEGKEPITGYKYESWHVRYVGVETAKEIMSRGICLEEYLGVDSEYKNTWPEDPCNPESGVSEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5UWT7</accession>
    <name>R5UWT7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00593</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000008">
        <property type="protein sequence ID" value="CCZ82788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82788">
        <property type="protein sequence ID" value="CCZ82788"/>
        <property type="gene ID" value="BN566_00593"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ82788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="142" mass="16627" checksum="5F34175A7A722289" modified="2013-07-24" version="1">MSFWISSILGKSAMGNKKQKVHENESGSHAPLHLSNLFFVYGSIIAWHLSLLLKKSDIMKPLFFFFHCRKTEKFRIGNFIFESPSKKSHTKIRNHSRAIDKIRHHFYNRRYSKTFLPERSLFDEKCLSILSGISYASTVLFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5UX08</accession>
    <name>R5UX08_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 8">UDP-glucose 4-epimerase</fullName>
            <ecNumber evidence="5 8">5.1.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN566_00653</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>UDP-alpha-D-glucose = UDP-alpha-D-galactose</text>
            <dbReference type="Rhea" id="RHEA:22168"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="EC" id="5.1.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 8">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 8">Carbohydrate metabolism; galactose metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 8">Belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.2" evidence="5 8"/>
    <dbReference type="EMBL" id="CBAR010000012">
        <property type="protein sequence ID" value="CCZ82848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00653"/>
    <dbReference type="EnsemblBacteria" id="CCZ82848">
        <property type="protein sequence ID" value="CCZ82848"/>
        <property type="gene ID" value="BN566_00653"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00214"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003978">
        <property type="term" value="F:UDP-glucose 4-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006012">
        <property type="term" value="P:galactose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd05247">
        <property type="entry name" value="UDP_G4E_1_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005886">
        <property type="entry name" value="UDP_G4E"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01179">
        <property type="entry name" value="galE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7 8" id="KW-0520">NAD</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-bd_dom" evidence="9">
        <location>
            <begin position="5"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ82848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="341" mass="37205" checksum="58A6171812EBCE13" modified="2013-07-24" version="1">MGEILVTGGAGFIGTHTCIALLQAGYSVVILDNLCNAKQESLLHLQEITGKSIPFYHMDIRNADGLRQIFQTHHISAVLHFAGLKAVGESVQQPLRYYQNNIGGMMTLLEELQKTDCRTLIFSSSATVYGTQHTAPYAETVTPLSASNPYGETKIISEQLLQDLCQADSDWHITALRYFNPIGAHESGLLGEEPHGIPSNIAPYLALVASGQLPYFRVYGNDYDTPDGTGVRDYVHVMDLAEGHVSALQYSMQHAGFSVFNLGTGSGCSVLELANMYEAVSGKPIPLKIFPRRAGDVATSYADVSKAKTVLGWTAKRNLQQMCADSWRFIQKQAQKGADSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>R5UX87</accession>
    <name>R5UX87_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3-isopropylmalate dehydratase small subunit</fullName>
            <ecNumber evidence="3">4.2.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Alpha-IPM isomerase</fullName>
            <shortName evidence="3">IPMI</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Isopropylmalate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">leuD</name>
        <name evidence="5" type="ORF">BN566_00718</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate</text>
            <dbReference type="Rhea" id="RHEA:32287"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:35121"/>
            <dbReference type="EC" id="4.2.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterodimer of LeuC and LeuD.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the LeuD family. LeuD type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.33" evidence="3"/>
    <dbReference type="EMBL" id="CBAR010000029">
        <property type="protein sequence ID" value="CCZ82918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00718"/>
    <dbReference type="EnsemblBacteria" id="CCZ82918">
        <property type="protein sequence ID" value="CCZ82918"/>
        <property type="gene ID" value="BN566_00718"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00071"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003861">
        <property type="term" value="F:3-isopropylmalate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01577">
        <property type="entry name" value="IPMI_Swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01032">
        <property type="entry name" value="LeuD_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033940">
        <property type="entry name" value="IPMI_Swivel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011824">
        <property type="entry name" value="LeuD/DmdB_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011827">
        <property type="entry name" value="LeuD_type2/HacB/DmdB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02084">
        <property type="entry name" value="leud"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02087">
        <property type="entry name" value="LEUD_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0432">Leucine biosynthesis</keyword>
    <keyword evidence="2 3" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aconitase_C" evidence="4">
        <location>
            <begin position="37"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ82918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="164" mass="17725" checksum="D2BFB871972B3655" modified="2013-07-24" version="1">MKFKGTVIKYGDNVDTDVIIPARYLNTIDKKELASHCMEDLDTTFTQRVKPGDIMVAGENFGCGSSREHAPIAIKESGISLVIAKSFARIFYRNSINIGLAIVECPEAAEAIEEGHTVEADLDNGILLDQTTGKSFHTAPFPPFIQTIITNGGLIASIKKGAVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXK3</accession>
    <name>R5UXK3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00610</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000008">
        <property type="protein sequence ID" value="CCZ82805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82805">
        <property type="protein sequence ID" value="CCZ82805"/>
        <property type="gene ID" value="BN566_00610"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ82805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="217" mass="25343" checksum="1228379303806F71" modified="2013-07-24" version="1">MKKHTYSIVLTDTVVQEVDRLAAQKGTNRSTMMNQILADYFSCKTPEKQMASVFSLIEQQMQNVFRVQLQASDAMISLHSALQYKYRPTVRYRVELLRIPTENSIGWLKISCRTQNQALLHSMQQFFRFWMHLELQTYPAYRTVPELYVLTENGMVRRLLRQNLETEEEVADAISSYIRQFDRLLKQYFAGLQQQVPAEALQAALEQSYLQMQAGLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXY3</accession>
    <name>R5UXY3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Peptide chain release factor 2</fullName>
            <shortName evidence="6">RF-2</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">prfB</name>
        <name evidence="8" type="ORF">BN566_00966</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="6">Methylated by PrmC. Methylation increases the termination efficiency of RF2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the prokaryotic/mitochondrial release factor family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000069">
        <property type="protein sequence ID" value="CCZ83183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00966"/>
    <dbReference type="EnsemblBacteria" id="CCZ83183">
        <property type="protein sequence ID" value="CCZ83183"/>
        <property type="gene ID" value="BN566_00966"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016149">
        <property type="term" value="F:translation release factor activity, codon specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00094">
        <property type="entry name" value="Rel_fac_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005139">
        <property type="entry name" value="PCRF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000352">
        <property type="entry name" value="Pep_chain_release_fac_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045853">
        <property type="entry name" value="Pep_chain_release_fac_I_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004374">
        <property type="entry name" value="PrfB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03462">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00472">
        <property type="entry name" value="RF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00937">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75620">
        <property type="entry name" value="SSF75620"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00020">
        <property type="entry name" value="prfB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00745">
        <property type="entry name" value="RF_PROK_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0488">Methylation</keyword>
    <keyword evidence="5 6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RF_PROK_I" evidence="7">
        <location>
            <begin position="245"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="modified residue" description="N5-methylglutamine" evidence="6">
        <location>
            <position position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="372" mass="42160" checksum="84A5C88D26B26E03" modified="2013-07-24" version="1">MVLLDELRVTMSDYQKDMDELYEVLGVAAATERYNVLQEEIAKEGFWDDLEHSQKVLQESKTLENRINSYKKMQSELEDIITLIELSIEEGETESTPDMNAECDAFIKKLEEMKLASLLTGEYDHSNAILTFHAGAGGTEAQDWAEMLYRMYNRWAERHHFKVTTLDYLDGDEAGLKSASILIEGENAYGFLKSESGVHRLVRVSPFDASGRRHTSFSAVEVMPEIDDNMEVDIRPEDIKMDVFRSSGAGGQHINKTSSAVRLTHLPTGIVVSCQTQRSQFQNKEYAMKMLKSKLVEIKEREHLDKIADIKGVQKEIAWGAQIRSYVFMPYTLAKDHRTSFENGNIQAVMDGDLDGFINAYLKALSSGTLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5UY01</accession>
    <name>R5UY01_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00986</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + O-phospho-L-tyrosyl-[protein] = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:10137"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:82620"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metallo-dependent hydrolases superfamily. CpsB/CapC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="3"/>
    <dbReference type="EMBL" id="CBAR010000070">
        <property type="protein sequence ID" value="CCZ83203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00986"/>
    <dbReference type="EnsemblBacteria" id="CCZ83203">
        <property type="protein sequence ID" value="CCZ83203"/>
        <property type="gene ID" value="BN566_00986"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016667">
        <property type="entry name" value="Caps_polysacc_synth_CpsB/CapC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39181">
        <property type="entry name" value="PTHR39181"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19567">
        <property type="entry name" value="CpsB_CapC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016557">
        <property type="entry name" value="Caps_synth_CpsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="SSF89550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="237" mass="27235" checksum="BFB6F655BFFD7BA5" modified="2013-07-24" version="1">MIDFHCHILPKMDDGSDSINTSLAMLREEHTQGVNRVVLTPHFYCEEESIEAFLQRRKKAFSALQTAISDQPHADDIPELCLGAEVAMSSALAQMDLQPLCISGTNVLLLELPYYRRFRLKDVETIINRNDIQVVFAHIERFSRLWNKETFAAVMELPVYKQINCSSLCHARWRQRRQLLRWLSDGEVHLLGTDAHNLTSRPVNFQAAAQLLQRKQRTAELETCMELAEQLTEDGVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYA5</accession>
    <name>R5UYA5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01089</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83308">
        <property type="protein sequence ID" value="CCZ83308"/>
        <property type="gene ID" value="BN566_01089"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="409" mass="48041" checksum="6CF43B6400A27798" modified="2013-07-24" version="1">MNPTEEFEIYQFLCKEAEAYVSLSQLLKDAHLTMEAASSAVADFRKQFQDGLQHWQISNQNPAAISLGLLQALQEHPGDLQLSFLYAAWITDRGLLFDWHPENRSEEQDIRVWLKQSKTVSEFQAFLQEQAIQKAQLPIFKQAAKKFQRNAEKGTDERDAIFYLSIQNPFFYAGRKSTAYVENIGELLRLLNGNPQLQAAKPYIIFAILSRKHGMMMNREHFIPNLQAAFQYQDYHIDFDNGKNFDNYQCYIELYETLRQFYRDDSNVNLPFCDYCFANLCPLSRWYYENCEPNEQIPMNIQQKAEMITAMIFPDLYDDSEDVEMEAIPEEWEEQLAEHPEWLTEFLQACAAETDRMAVVSHLWTEPPEQQLQQAAEQVFYFYAKDFLRKQIWEAAEWLLPDFETISSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYC0</accession>
    <name>R5UYC0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Amino acid ABC transporter periplasmic amino acid-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_01104</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the bacterial solute-binding protein 3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000084">
        <property type="protein sequence ID" value="CCZ83323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01104"/>
    <dbReference type="EnsemblBacteria" id="CCZ83323">
        <property type="protein sequence ID" value="CCZ83323"/>
        <property type="gene ID" value="BN566_01104"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018313">
        <property type="entry name" value="SBP_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001638">
        <property type="entry name" value="Solute-binding_3/MltF_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00497">
        <property type="entry name" value="SBP_bac_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00062">
        <property type="entry name" value="PBPb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01039">
        <property type="entry name" value="SBP_BACTERIAL_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004394478" evidence="4">
        <location>
            <begin position="25"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="PBPb" evidence="5">
        <location>
            <begin position="42"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ83323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="271" mass="29713" checksum="AF0CADB06E33C09C" modified="2013-07-24" version="1">MMWMKRLVAATAAIAMLGALSSCGSKDDSSTANNGNGLSDNKFVVGFDAAFPPYGYQDDSGEYVGFDLDLAQEVCSRNSWELVKMPIDWDSKDMELNSGTISCIWNGFTMNGREDDYTWSDPYVDNSQVFVVKSDSGITTFDDLSGKTVAVQTDSSAEAALNEEDNAALKDSFKELLVVGEYNTAFLNLESNAVDAIAMDIGVAKYQLESRDGDFTILDQPLAAEQYAVGFKKGNETLRDQVQNTLNEMKSDGTFKTIAEKWDLQDSVILD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYD6</accession>
    <name>R5UYD6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sucrose-6-phosphate hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01119</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000089">
        <property type="protein sequence ID" value="CCZ83338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01119"/>
    <dbReference type="EnsemblBacteria" id="CCZ83338">
        <property type="protein sequence ID" value="CCZ83338"/>
        <property type="gene ID" value="BN566_01119"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.115.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001362">
        <property type="entry name" value="Glyco_hydro_32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013148">
        <property type="entry name" value="Glyco_hydro_32_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00251">
        <property type="entry name" value="Glyco_hydro_32N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00640">
        <property type="entry name" value="Glyco_32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="SSF75005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyco_hydro_32N" evidence="2">
        <location>
            <begin position="47"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="261" mass="30392" checksum="3A6FBE01BB347869" modified="2013-07-24" version="1">MTGEPPKPSCHFSIKLKKSAYSISSRRIKQKSNTTSDKCSTFTVPLKAPNCWINDPNGFIWYKGQYHLFYQCFPYAPQWGRMHWGHAVSKDLVNWEKKGIALYPTKIDDRSGCFSGSAVEQDRTLYLFYTGVNYLEENPEDINQCRNDQFLAAQMMISSDDGMTFDNVKRKKTVIPPITEKQIGSKTNTRDPKVWRGKDVWYLVLGSSVDHKGRLLIYKSSDLQNWTYLSHVKKDNFGWMWECPDYFEMDRVRLMKTEIAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYK4</accession>
    <name>R5UYK4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00897</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000059">
        <property type="protein sequence ID" value="CCZ83110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83110">
        <property type="protein sequence ID" value="CCZ83110"/>
        <property type="gene ID" value="BN566_00897"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="66" mass="7569" checksum="CA407D204987D64D" modified="2013-07-24" version="1">MAKGCFRRTDRVCALCRHWNGAMGSTTIQPVLGGRFQVEMKEKETCYQRTGQTQATFACSKFEPRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYV1</accession>
    <name>R5UYV1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01003</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000075">
        <property type="protein sequence ID" value="CCZ83220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83220">
        <property type="protein sequence ID" value="CCZ83220"/>
        <property type="gene ID" value="BN566_01003"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="65" mass="7284" checksum="5A6BD662635756BA" modified="2013-07-24" version="1">MYIRYTNYKTGMQLGENDSVLMSLQEFLDGGKDLEDVPVTITPDGEPPTEETEEEPDVSEEEAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYZ1</accession>
    <name>R5UYZ1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Tetratricopeptide repeat protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01046</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01046"/>
    <dbReference type="EnsemblBacteria" id="CCZ83265">
        <property type="protein sequence ID" value="CCZ83265"/>
        <property type="gene ID" value="BN566_01046"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025117">
        <property type="entry name" value="DUF4037"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025191">
        <property type="entry name" value="DUF4125"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13228">
        <property type="entry name" value="DUF4037"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13526">
        <property type="entry name" value="DUF4125"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13176">
        <property type="entry name" value="TPR_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4037" evidence="1">
        <location>
            <begin position="393"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="768" mass="87158" checksum="7B254268D091C035" modified="2013-07-24" version="1">MQVQEILQQLDTLLKNSRLQEADVFLKEQIAAAEQAKDWQTALTLYNDQIGFFRDCGRFPESLAVGEKALECVEKLHLKQTIAHATTLLNVANAQRAAGMQEAALNSYQQVKQLYDALLSPTDSRVASYYNNLSLLYQEMGDWKASCTALEQALQIVTQQPGQEGRVAISKTNLASSLLHLHRVQDALPLLKSALSFFDGLSPSDFHYSAALAAMADAQYQLQDYEQAVWYYEAALSEMELHMGRGAAYQIVQENADHAYEKLGGKPIRKGLELCRQYYETFGKPMLQRMFPDIWEQLTIGLAGEGSECFGYDDAYSQDHDFGAGFCIWVPDEMAEAKITALQQAYNLLPKTYCGITRKTMPQGEHRVGVCRTSDFYQRLLGISGVPKTEQEWLQIEEAQLAAATNGVLFKDSNQAFSKIRNQLQQGYPEAVRLRRLAQETAWMAQRGQYNVPRLLQRNDSLTTMLAFSHFAESAMRAAHLCARNYAPYYKWLLHSTEQLPQGAELAALLQKSTTLPLKQWETEIIAPVCAIIAKQMKEQGISTQEESYMQVHAEAAAQQAALLEQKEVLVEKIVRLEFSEFDKVKNEGVRAGCQDDWETFSIMRKSQYLTWELPMLQQYWAELQSASQRGWNLLTEKYARMMASTAPEAYAALKKELPPVSEQKQALCEAIISIQVSWMEDFAKQYPNLAGQARKIHTSEDTEWQTSYETYLRGELLTYSEDLLQAYGAWIVTLYRAGKNLAEQIMTNTVHFYQYLSLEAAEKQVQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZ66</accession>
    <name>R5UZ66_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Predicted permeases</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_00145</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the auxin efflux carrier (TC 2.A.69) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000146">
        <property type="protein sequence ID" value="CCZ83618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00145"/>
    <dbReference type="EnsemblBacteria" id="CCZ83618">
        <property type="protein sequence ID" value="CCZ83618"/>
        <property type="gene ID" value="BN566_00145"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004776">
        <property type="entry name" value="Mem_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03547">
        <property type="entry name" value="Mem_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="66"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="127"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="160"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="200"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="231"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="286"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="308" mass="33462" checksum="8339AD9E2B017AF9" modified="2013-07-24" version="1">MAGIILQQAIIMLILNVVGMIAYYTGIVSREGGKQLSNVVLEIVTPVIIVHAYLEEEYDSRLVVNLLLTFVLAAASYVIAILISMLCFREKSAGKLAPIERFSATYSNCGFMGIPLASALFGAEGVFYVTAFLTIFFLFSWTHGVMQLTQTKDFKKALKVLRSPTVIGIAIGLILYFVQLPMPGLLMDTMGYISDLNTPLAMIASGVSVAQANLLGALCTKRIYWVSFVKLLVIPLAVMAMCLCLPFVSMEVRTVVLLLTAAPAAAMCTLQCQVHGLNDIYASQIFAATTILSMATMPLLIQIFSMLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZ75</accession>
    <name>R5UZ75_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00122</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000090">
        <property type="protein sequence ID" value="CCZ83340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83340">
        <property type="protein sequence ID" value="CCZ83340"/>
        <property type="gene ID" value="BN566_00122"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025466">
        <property type="entry name" value="DUF4317"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14199">
        <property type="entry name" value="DUF4317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="415" mass="46979" checksum="2EC64A2AB0D91AE7" modified="2013-07-24" version="1">MNKKDLRELKKNFSDTSDLFTMNHVVTAFVDAEKNIRCQNSQAYHDIPQEEAFCYWKTFKKVLSGSLGKGLLEYEFPKEAYEEGAPQQILYQALNTKLKDESDVSILLDQIVRQMDATSMYAILVAHCTYTVFNKTRNDEKNPYDSLDYSFLAVAICPVEVRTDGLIYNETDNEIMKKIDTDRIVAEVPSDGFLYPTFTGRGPDVNHVLYYTRKPKEVNVSLVEQVLGCKFTYTAQQEKEQFQEMLQDILGEELNYNTITAVNERIQSVAEDPEPSADLPVIDDIQIRDILLDAGVSEEKAEEMQTAYREKVQNHPLSADNLVDKKTVITAPGISVQVKKDFTSCVHLQNVDGHRYLMIDLDDPDIQVNGMSVEIPETTLPPTSEEAMLEQLGTDFVSLESTDSAADEPDSDLPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZF6</accession>
    <name>R5UZF6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Endo-1 4-beta-xylanase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_01181</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000096">
        <property type="protein sequence ID" value="CCZ83405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01181"/>
    <dbReference type="EnsemblBacteria" id="CCZ83405">
        <property type="protein sequence ID" value="CCZ83405"/>
        <property type="gene ID" value="BN566_01181"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045493">
        <property type="term" value="P:xylan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010502">
        <property type="entry name" value="Carb-bd_dom_fam9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002105">
        <property type="entry name" value="Dockerin_1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044846">
        <property type="entry name" value="GH10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031158">
        <property type="entry name" value="GH10_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001000">
        <property type="entry name" value="GH10_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31490">
        <property type="entry name" value="PTHR31490"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06452">
        <property type="entry name" value="CBM9_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00404">
        <property type="entry name" value="Dockerin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00331">
        <property type="entry name" value="Glyco_hydro_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00633">
        <property type="entry name" value="Glyco_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00591">
        <property type="entry name" value="GH10_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51760">
        <property type="entry name" value="GH10_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3 8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0858">Xylan degradation</keyword>
    <feature type="domain" description="GH10" evidence="6">
        <location>
            <begin position="1"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="7">
        <location>
            <begin position="564"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="638" mass="69926" checksum="CF124FFF7BD8CDCA" modified="2013-07-24" version="1">MQVYGDQSYILKAFEYARKYAPAGCKLFYNDYNEYNTAKMNYIVSDILKPLIAAGTIDGMGMQSHISMSTPTIEQYKKAMQTYSDLGLEIQVTELDVSQKSNAVADQLELAQRYQDCFKMYKEMKASGVNLSAVVIWGITDSTSWIGGYPLLFDKDYQAKPSYYAVVDTDSEVEKIQTMNAYQYASDADLEQAFAIQTPQTLQRLSTDGNTSTSDMTFKAAWNADGLVIRVSNLDPGLSSLSVYGSTTDQQGELLKMVTDFDGKDSMDISVPLTGKAGSIVYLDLVAESNVTAYVQKTDGTYEDVHFHSLYAWNDMDFIKQFMVNGIITKMVPTCGIVTLKAQPKSATATYGTPTIDGTIDSVWDDAPTIDVNTFSVGNGATGTAKMLWDENNLYVLTEVTDPVLSKSSANAYEQDTVEVFFDENNHKTDYYETDDLQARVNYDNEKTITDGLSTDRFVSATAKTDKGYLVEMAIPFGIAPFKNGQVLGFDVQVNDDGTGDGKRTAISNWNDLTGMGYTSTAGYGVLTLTGGSSETTTSATTTTGTSTTTTTTATTVTTTDTLENINYGDVNLDGVVDLADSIVLNKYLAQVLTLSDMALKNADVNTDQNLTEKDTTILMRFILQDKDYPELPHVDAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZG4</accession>
    <name>R5UZG4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Exopolyphosphatase-related proteins</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01186</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000097">
        <property type="protein sequence ID" value="CCZ83410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01186"/>
    <dbReference type="EnsemblBacteria" id="CCZ83410">
        <property type="protein sequence ID" value="CCZ83410"/>
        <property type="gene ID" value="BN566_01186"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="SSF64182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHH" evidence="1">
        <location>
            <begin position="17"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="DHHA1" evidence="2">
        <location>
            <begin position="232"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="319" mass="34706" checksum="45AA87AF421E929A" modified="2013-07-24" version="1">MNIAETAAQLQTIQQAMILIHQSPDGDCIGSGYALAALLRQMGCHAVVRCSDPIPERYAFLAVEETPDTVDEDAAVILSVDVADRKLLGDLNEPYGGRVALAIDHHIMHRSFAKECCLYPKAAAACEIVYAIAKELPVKLTPQIATCLYTGMATDTGCFQFDNVTPHTLRIVADLMEQFPEIHYAWINRAMFAVKSMGRLRVEQKLIDQLHTSCNGKCVMICITLAFMEQYGLDPLDLDGLAGFPLQVEGAEVGITLKERDPNVFKVSMRSARTVDVAAICKEFGGGGHIKAAGCLIEGTAESVMQQLQTAVERSLAES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZK6</accession>
    <name>R5UZK6_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CbiA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01523</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000162">
        <property type="protein sequence ID" value="CCZ83788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01523"/>
    <dbReference type="EnsemblBacteria" id="CCZ83788">
        <property type="protein sequence ID" value="CCZ83788"/>
        <property type="gene ID" value="BN566_01523"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CbiA" evidence="1">
        <location>
            <begin position="8"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="221" mass="24607" checksum="C9D39CFE7F9F86F8" modified="2013-07-24" version="1">MKRITLITGHYGSGKTNFAVNLALRQAAEGKKVTVVDLDIVNPYFRTADFAELFEQNGIHLEKTLYANTSLDIPAISFDLARLAYEEECLIIDVGGDDAGATALGRYAEMLRSYQAELDMWYVVNRYRYLTAQPQEALTLLYEVQQAARLQPTGIVNNSNLGSETTAELILEAVPYAKTIAEAAGLPLICTTYREDLPVQVENGFPVQVYVKPVWEQDDAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZM0</accession>
    <name>R5UZM0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01538</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000164">
        <property type="protein sequence ID" value="CCZ83803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01538"/>
    <dbReference type="EnsemblBacteria" id="CCZ83803">
        <property type="protein sequence ID" value="CCZ83803"/>
        <property type="gene ID" value="BN566_01538"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004406587" evidence="2">
        <location>
            <begin position="30"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="503" mass="53561" checksum="7CB61847FD39493B" modified="2013-07-24" version="1">MNKLTRTLALLAALAMTATAFVGCGDDKNSSSSSSEAATTVAEESSEETSAAEESSEETSAEESSEEASDSDATASTELGAVSMPDTGDALTVLCWTDTDLNAMIPVVEKAYPDMAGKIKYQNVGSAGTEAMEQYLTYFSSGSDVDLYICDADWVLSYENNDENSAPLESVGITADMYSDAYSYTVAMGTDQNGVLKGASWQAAAGGYCYRTDLAEQYLGVKTPEEMQAKVADWDTFWATAKEVYDASGNKTAMADTLGGVWRAYSAGNRSTAWVQDGKITADNAKACLGDFINMAKTNYDAGYIGTVDQWTDDWYAIGQADGAMADATMGYFFPSWSLAAGGQLESAEGGESGSTYGKYAITEGPTGWFWGGSWICVSPNCDNGTFAGQFIKAMTVDDATMKEYCESHSDFVNNKSVMKEVVEGGKNSNPLFKDGQDQFAVLYDSADAIDLDGIATQYDGTINSKLQDAVTAYCTGDLADEQACYDEFLDTVASAISDITVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZW3</accession>
    <name>R5UZW3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CobW/P47K family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01279</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000119">
        <property type="protein sequence ID" value="CCZ83510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83510">
        <property type="protein sequence ID" value="CCZ83510"/>
        <property type="gene ID" value="BN566_01279"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011629">
        <property type="entry name" value="Cbl_biosynth_CobW-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07683">
        <property type="entry name" value="CobW_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobW C-terminal" evidence="1">
        <location>
            <begin position="28"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="138" mass="15574" checksum="60784BB35CE5B9C4" modified="2013-07-24" version="1">MYLFDKVQTTSRATHYFMHIHLPDERIPEILQAMLNDPNCGTIYRIKGALPLKQNCWLSINLTQKKAELQTVSNGQSKGALPLQQNRWLSINLTQKKAELQTVSNGQSVLIVIGDQLNRAALDAHLKAFNTDPDYVSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5V040</accession>
    <name>R5V040_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF6472 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01687</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000176">
        <property type="protein sequence ID" value="CCZ83958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01687"/>
    <dbReference type="EnsemblBacteria" id="CCZ83958">
        <property type="protein sequence ID" value="CCZ83958"/>
        <property type="gene ID" value="BN566_01687"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045525">
        <property type="entry name" value="DUF6472"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20076">
        <property type="entry name" value="DUF6472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6472" evidence="1">
        <location>
            <begin position="6"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="59" mass="7286" checksum="90B53233EBD16CB9" modified="2013-07-24" version="1">MQNQICCETCAYYTYDEEWECYVCEQNLDEDEYVRLVEGHYRSCPYYRDGDCYKIARKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5V081</accession>
    <name>R5V081_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01396</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000148">
        <property type="protein sequence ID" value="CCZ83660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83660">
        <property type="protein sequence ID" value="CCZ83660"/>
        <property type="gene ID" value="BN566_01396"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="SSF52980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="185" mass="21717" checksum="0B5BD1619B06AE01" modified="2013-07-24" version="1">MTDRKAFAKKVKVLCDTREQCNQHIIQYLHANGIATESRKLDFGDYSFEINGKCFERSCIVERKGSVDELFGNFVHDRERIQKEFDAAAKNAQHMELILEGVTSEEELKAFEIPEKQMIAQNRKVKRIGETVYFALRSLRSGNRCGLQVSFVRKEDTAKKLLEIFYYYYRNYERAVAPLRKEQEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0C9</accession>
    <name>R5V0C9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Diaminopimelate decarboxylase</fullName>
            <shortName evidence="2">DAP decarboxylase</shortName>
            <shortName evidence="2">DAPDC</shortName>
            <ecNumber evidence="2 3">4.1.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">lysA</name>
        <name evidence="6" type="ORF">BN566_01460</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Specifically catalyzes the decarboxylation of meso-diaminopimelate (meso-DAP) to L-lysine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>H(+) + meso-2,6-diaminoheptanedioate = CO2 + L-lysine</text>
            <dbReference type="Rhea" id="RHEA:15101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:57791"/>
            <dbReference type="EC" id="4.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 2 3">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 3">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; L-lysine from DL-2,6-diaminopimelate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Orn/Lys/Arg decarboxylase class-II family. LysA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.20" evidence="2 3"/>
    <dbReference type="EMBL" id="CBAR010000158">
        <property type="protein sequence ID" value="CCZ83725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01460"/>
    <dbReference type="EnsemblBacteria" id="CCZ83725">
        <property type="protein sequence ID" value="CCZ83725"/>
        <property type="gene ID" value="BN566_01460"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00027"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008836">
        <property type="term" value="F:diaminopimelate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06828">
        <property type="entry name" value="PLPDE_III_DapDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02120">
        <property type="entry name" value="LysA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009006">
        <property type="entry name" value="Ala_racemase/Decarboxylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002986">
        <property type="entry name" value="DAP_deCOOHase_LysA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022643">
        <property type="entry name" value="De-COase2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022644">
        <property type="entry name" value="De-COase2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000183">
        <property type="entry name" value="Orn/DAP/Arg_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02784">
        <property type="entry name" value="Orn_Arg_deC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00278">
        <property type="entry name" value="Orn_DAP_Arg_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01181">
        <property type="entry name" value="DAPDCRBXLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01179">
        <property type="entry name" value="ODADCRBXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50621">
        <property type="entry name" value="SSF50621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="SSF51419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01048">
        <property type="entry name" value="lysA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 3" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="2 3" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 3" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Orn_Arg_deC_N" evidence="5">
        <location>
            <begin position="40"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="Orn_DAP_Arg_deC" evidence="4">
        <location>
            <begin position="299"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="2">
        <location>
            <begin position="291"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="335"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="363"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="391"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="2">
        <location>
            <position position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ83725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="433" mass="48002" checksum="E9C1A31BD86F4F4D" modified="2013-07-24" version="1">MFVSEDLQVNAQGHLTIGGQDTVELAKQYGTPLYVMDEDLIRKHCRSFKQSMDKFYQGKGLVCYASKAFSCKAIYRVMQEEGLGVDVVSGGELYTAMQTGFDSSKICFHGNNKTTEELQMALDYQVGRIIVDNIYELHQLEQMCQEQGKTARIMFRIKPGVDAHTHNFIRTGQIDSKFGFALETGEAMEAVKEALQCKSLHLCGLHCHIGSQILDTNGFEAAAEVMMQFIATIQKELHITIEELNLGGGFGICYTEQDDPIPYEQYMEAVSKVIHRCSEEYGVPQPFILIEPGRSIVAPAGITLYTVGGKKVIPNIRTYVSIDGGMCDNPRYILYQSAYDAIVANKADQPKDTKVTIAGKCCESGDLIGENMPLQACEPGDIIAVCATGAYNYSMSSNYNRLQKPAVVFVKDGTSRVVVKRETLQDLVRNDCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0L4</accession>
    <name>R5V0L4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Acetyltransferase (GNAT) family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01858</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000191">
        <property type="protein sequence ID" value="CCZ84133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01858"/>
    <dbReference type="EnsemblBacteria" id="CCZ84133">
        <property type="protein sequence ID" value="CCZ84133"/>
        <property type="gene ID" value="BN566_01858"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="15"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="153" mass="17418" checksum="358267609D863C80" modified="2013-07-24" version="1">MEYILGTIADIPALIDMRTAYILEDFGELSPEDEAVMRKTLPDYFSEHLNRDCIAFLAKDGETVVSVALLMILQKPCHPKMIHGKMGEVLSVYTLPAYRRQGIAQHNMQRLVDYARANGLDYVNLSATKAGYPVYQKVGFSERQSHYTEMQIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0N1</accession>
    <name>R5V0N1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Thiamine monophosphate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01878</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000193">
        <property type="protein sequence ID" value="CCZ84153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01878"/>
    <dbReference type="EnsemblBacteria" id="CCZ84153">
        <property type="protein sequence ID" value="CCZ84153"/>
        <property type="gene ID" value="BN566_01878"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00564">
        <property type="entry name" value="TMP_TenI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036206">
        <property type="entry name" value="ThiamineP_synth_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022998">
        <property type="entry name" value="ThiamineP_synth_TenI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02581">
        <property type="entry name" value="TMP-TENI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51391">
        <property type="entry name" value="SSF51391"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TMP-TENI" evidence="1">
        <location>
            <begin position="5"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="198" mass="21717" checksum="80FC33B8F1B70835" modified="2013-07-24" version="1">MKILCITNQALCKGDYLQQLQKIAAAKPDAMILREKQLSETAYQALAEQVQRICQEQGVQLICNHQISVAERLSLPVQISVSQLEQVSALSVPFGVSVHKLEEAKQAEQAGASWVIAGHVFATACKPDLSPRGLSFLQEIKRAVSIPVYGIGGIHVQNAAQVQAIGADGICMMSDWMQAEEPEQLMQQLRAIKPEEQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0W4</accession>
    <name>R5V0W4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase</fullName>
            <ecNumber evidence="5">2.7.8.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Phosphatidylglycerophosphate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">BN566_00012</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">This protein catalyzes the committed step to the synthesis of the acidic phospholipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a CDP-1,2-diacyl-sn-glycerol + sn-glycerol 3-phosphate = 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycero-3'-phosphate) + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12593"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:60110"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="2.7.8.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Phospholipid metabolism; phosphatidylglycerol biosynthesis; phosphatidylglycerol from CDP-diacylglycerol: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.5" evidence="5"/>
    <dbReference type="EMBL" id="CBAR010000197">
        <property type="protein sequence ID" value="CCZ84243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00012"/>
    <dbReference type="EnsemblBacteria" id="CCZ84243">
        <property type="protein sequence ID" value="CCZ84243"/>
        <property type="gene ID" value="BN566_00012"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00084">
        <property type="reaction ID" value="UER00503"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008444">
        <property type="term" value="F:CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006655">
        <property type="term" value="P:phosphatidylglycerol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004570">
        <property type="entry name" value="Phosphatidylglycerol_P_synth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000847">
        <property type="entry name" value="Phos_ph_gly_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00560">
        <property type="entry name" value="pgsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="14" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 16 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="12"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="129"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00014944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00033018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CCZ84243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="191" mass="20732" checksum="73570C8B76973574" modified="2013-07-24" version="1">MNLPNKLTCLRVLLIPVFLIFLYIGAIPCHFLLALVVFAAASITDALDGKIARARGLVTNFGKFLDPLADKVLVITALLAFIELDKMQISAIPVMIIVAREFMVSGLRMLTASEGVVVAAGIWGKLKTAFTMVTIVAILIYCSLTGDFNCAIPEAVKTWGLNGLLWISTVLTIISGCGYLKGYWSYINTDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0Z4</accession>
    <name>R5V0Z4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein related to enterotoxins of other Clostridiales</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_00202</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000205">
        <property type="protein sequence ID" value="CCZ84273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84273">
        <property type="protein sequence ID" value="CCZ84273"/>
        <property type="gene ID" value="BN566_00202"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018337">
        <property type="entry name" value="Cell_wall/Cho-bd_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039564">
        <property type="entry name" value="Peptidase_C39-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01473">
        <property type="entry name" value="Choline_bind_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19127">
        <property type="entry name" value="Choline_bind_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13529">
        <property type="entry name" value="Peptidase_C39_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51170">
        <property type="entry name" value="CW"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004394707" evidence="3">
        <location>
            <begin position="34"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="repeat" description="Cell wall-binding" evidence="2">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="repeat" description="Cell wall-binding" evidence="2">
        <location>
            <begin position="291"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="repeat" description="Cell wall-binding" evidence="2">
        <location>
            <begin position="372"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_C39_2" evidence="4">
        <location>
            <begin position="462"/>
            <end position="595"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ84273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="621" mass="68193" checksum="4ACF426D4E084461" modified="2013-07-24" version="1">MAQKQYSRSIARLVLVAGMCFTAIGLCSVSASAIDNTELLQPVAIKEDSLQPIGQSVLLADVTEENWYEGTSGSLYYCYADGTYATGITTLPNEETYLFALDGTLKTGWQTIDGVRYYFDLTTGQKQFGWISHSNRLYYVFPETGKQVGEAVIDGTPYVFDEFGVLQTGIYTCEDGTIAGCAEDATPYTGFVTDETSQATYYFNENGIAQTGFQIVDGATYYLGEDGVLRTGLTSIDDSVYDFGTDGVMQTGLVTMEDGTVLFFGADGKQAMGLQTVDGKTYYFNDLGMAVNTKQTIDGKTYYFGADGSMQTGLVTLNKDVYYFGKDGNMQTGFVTMEDGTVLFFGADGKQAMGLQTVNGKTYYFSDLGMAVNAKQTIDGKTYYFGADGSMQTGKVTLQDGSYYFGKNGEQVTGWLTLDGSEYYFDLTTGKMATGTVTLPDGTYIFDENGVLQKKQNGISYDVPYYNQADPRWGSTYIGTKTIAQVGCLVTSLAMTQSYHTDTEILPDEMRYQMQFDNNSILWAPIIALGYDLEGCSGLSTYAFCQRLYTLLEEHGPVIVGSDNVYGGSHYVVVTGCTNTDTSTLKTSDFTMNDPGFSNRTTLDEHLAAYPTWFRFFYMNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5V173</accession>
    <name>R5V173_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00251</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plasmid mobilization pre family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000216">
        <property type="protein sequence ID" value="CCZ84338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00251"/>
    <dbReference type="EnsemblBacteria" id="CCZ84338">
        <property type="protein sequence ID" value="CCZ84338"/>
        <property type="gene ID" value="BN566_00251"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001668">
        <property type="entry name" value="Mob_Pre"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01076">
        <property type="entry name" value="Mob_Pre"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="126"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="205"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="299" mass="34200" checksum="1BB6D77BF204BE5A" modified="2013-07-24" version="1">MQIGKMEDSGYGTEDFQLVADCLKEYMESFQDRNPNFYVFNAVLHMDEATPHLHIDYIPVGHYKRGQDTQNGIAQALKEMSFGEGKQAIARWRAAEVEVLNKNCLEHGIKPLAPEKARGTLEVEEYKEQRRQADELAEQNAQVKAELGEKREMLKAVKEKTAKLAEIDSIETGKTVFGGKVTVSQEDWQNITDLAKKEVASQKQTKKLRKERDEAVRERDELKAKLSAVSSELATYKKAEEQKGLFTREKLKKEAKRISREDELSRELRKAKAFISACGLSSDFQQYKYNSTTRKNVLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1B1</accession>
    <name>R5V1B1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GMP synthase [glutamine-hydrolyzing]</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00280</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000222">
        <property type="protein sequence ID" value="CCZ84373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00280"/>
    <dbReference type="EnsemblBacteria" id="CCZ84373">
        <property type="protein sequence ID" value="CCZ84373"/>
        <property type="gene ID" value="BN566_00280"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003922">
        <property type="term" value="F:GMP synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001674">
        <property type="entry name" value="GMP_synth_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00958">
        <property type="entry name" value="GMP_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GMP_synt_C" evidence="1">
        <location>
            <begin position="10"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="102" mass="11636" checksum="A6C649BA575493EB" modified="2013-07-24" version="1">MQDADYIFREEIAKAGLDRSIHQYFAVLTNMRSVGVMGDGRTYDYTLALRGVTTTDFMTADFARIPYEVLETVSARIVNECKCINRVVYDVTTKPPATIEWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-09-29" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1B2</accession>
    <name>R5V1B2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01746</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000180">
        <property type="protein sequence ID" value="CCZ84020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84020">
        <property type="protein sequence ID" value="CCZ84020"/>
        <property type="gene ID" value="BN566_01746"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="48"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="228"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="260"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="305"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="333"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="361" mass="39935" checksum="71912B2BC561675B" modified="2013-07-24" version="1">MLQLFRKKQQFLAVSYATILHCLVDFCCIYLMMQLVFPACRLTSLSNWLLYTMLYNFIAFAGQLPVGIFGDWLQRNMNLIAVGLLFLLVAYCSALFGFPLLLIVLLLGIGNACFHVGGGRAAMEWKENACQPVGIFVATGAFGVFGGKWISGNGMNWIAPMIVVLSMALLIQLIFCRNSEERNLPKQEFSMKPMQGSWSLLMAFCCIFVVVLRSFAGSVMHFPWAKNWLALFLTLAIVLGKAGGGILADRFGIRPTIIGSLTACALLFLGANQFAICGLLAVFLFNMTMPITLSLLGMQFPHAKGMAFGALTFALFVGLVPSYFCNINDFAKPWIYCLLAVLSLVILYAPMLRNAKKRNIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1B5</accession>
    <name>R5V1B5_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase</fullName>
            <ecNumber evidence="4">2.4.2.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">N(1)-alpha-phosphoribosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN566_01751</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5,6-dimethylbenzimidazole + nicotinate beta-D-ribonucleotide = alpha-ribazole 5'-phosphate + H(+) + nicotinate</text>
            <dbReference type="Rhea" id="RHEA:11196"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15890"/>
            <dbReference type="ChEBI" id="CHEBI:32544"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:57918"/>
            <dbReference type="EC" id="2.4.2.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Nucleoside biosynthesis; alpha-ribazole biosynthesis; alpha-ribazole from 5,6-dimethylbenzimidazole: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CobT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.21" evidence="4"/>
    <dbReference type="EMBL" id="CBAR010000180">
        <property type="protein sequence ID" value="CCZ84025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01751"/>
    <dbReference type="EnsemblBacteria" id="CCZ84025">
        <property type="protein sequence ID" value="CCZ84025"/>
        <property type="gene ID" value="BN566_01751"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00061">
        <property type="reaction ID" value="UER00516"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008939">
        <property type="term" value="F:nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02439">
        <property type="entry name" value="DMB-PRT_CobT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1610.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003200">
        <property type="entry name" value="Nict_dMeBzImd_PRibTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017846">
        <property type="entry name" value="Nict_dMeBzImd_PRibTrfase_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023195">
        <property type="entry name" value="Nict_dMeBzImd_PRibTrfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036087">
        <property type="entry name" value="Nict_dMeBzImd_PRibTrfase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02277">
        <property type="entry name" value="DBI_PRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52733">
        <property type="entry name" value="SSF52733"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03160">
        <property type="entry name" value="cobT_DBIPRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="7 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ84025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="351" mass="37451" checksum="4A4E9F771FE196D8" modified="2013-07-24" version="1">MTLSECCMQIQPLDEMAMQAAQQHWNQLAKPLHSLGRLEDMIVQLAGIHRSAQLEQRKKVVLIFCADNGIVEEGVTQTGQEVTATVAENFTKGIATVNSLAQVCQADVMPIDIGIARDMTCNGIINRKIAYGTKNFAKEPAMTRTEAEQAILTGIQLVREKKEQGYNLIITGEMGIGNTTTSSAVFSILSQITPELVTGKGAGLSKAGIQRKIQVIQAAIQKYQPDPEDVIDVLSKVGGLDICGMMGAFLGGAIYRVPVLIDGFISAVAANCAVRYAPACKPFLYASHCSAESAGKLALEALQLPAYLECGMCLGEGTGAVIGAKLFDFALAAYTEAASFQQAQVTPYQPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1D2</accession>
    <name>R5V1D2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00295</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000222">
        <property type="protein sequence ID" value="CCZ84388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00295"/>
    <dbReference type="EnsemblBacteria" id="CCZ84388">
        <property type="protein sequence ID" value="CCZ84388"/>
        <property type="gene ID" value="BN566_00295"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043721">
        <property type="entry name" value="DUF5662"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18907">
        <property type="entry name" value="DUF5662"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="187" mass="22151" checksum="7A47639596F665C0" modified="2013-07-24" version="1">MLRNLWHHFLLITRHRHKVIAHCKKAGILWQGLRHDLSKYSPTEFIPGVKYYQGNRSPNEKEREILGYSLAWLHHKGRNRHHFEYWTDYNPVEKKVMPVKMPARFLVEMFCDRLAASKIYKGEQYTDQAPLEYFQNSTARKNGCLHPETERQLMQLLEILAKEGEPAAFATARALLKAEKQQKKKEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1R4</accession>
    <name>R5V1R4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">3-oxoacyl-[acyl-carrier-protein] reductase</fullName>
            <ecNumber evidence="6">1.1.1.100</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_00387</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the NADPH-dependent reduction of beta-ketoacyl-ACP substrates to beta-hydroxyacyl-ACP products, the first reductive step in the elongation cycle of fatty acid biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>a (3R)-hydroxyacyl-[ACP] + NADP(+) = a 3-oxoacyl-[ACP] + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:17397"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:9945"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:78827"/>
            <dbReference type="EC" id="1.1.1.100"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 6">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.100" evidence="6"/>
    <dbReference type="EMBL" id="CBAR010000240">
        <property type="protein sequence ID" value="CCZ84488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00387"/>
    <dbReference type="EnsemblBacteria" id="CCZ84488">
        <property type="protein sequence ID" value="CCZ84488"/>
        <property type="gene ID" value="BN566_00387"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008202">
        <property type="term" value="P:steroid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011284">
        <property type="entry name" value="3oxo_ACP_reduc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01830">
        <property type="entry name" value="3oxo_ACP_reduc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="6" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 6" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0753">Steroid metabolism</keyword>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="154"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="62"/>
            <end position="63"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="154"/>
            <end position="158"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR611284-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR611284-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU366074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ84488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="246" mass="25972" checksum="9F3CBF7EA313D293" modified="2013-07-24" version="1">MATALITGASQGIGACIAERLAKDGYNIAINHYPSDGDKANAEKVAETCRSYGVEVALFPANVADYAQCEEMVKAVKKQFGGIDALVNNAGITKDGLLLRMSEEQYDAVIAVNQKSVFNMMKLVGAVMLRQKHGRIVSLASVAGLYGNPGQMNYSASKAAIIGMTKTAAKELGSRGITVNAVAPGFIKTPMTDALTDEQRDKMLGLVAMGRYGQPEEIASVVSFLCSDDASYVTGQVIEISGGLMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1R9</accession>
    <name>R5V1R9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Biotin carboxylase</fullName>
            <ecNumber evidence="5">6.3.4.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Acetyl-coenzyme A carboxylase biotin carboxylase subunit A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_00392</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">This protein is a component of the acetyl coenzyme A carboxylase complex; first, biotin carboxylase catalyzes the carboxylation of the carrier protein and then the transcarboxylase transfers the carboxyl group to form malonyl-CoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + hydrogencarbonate + N(6)-biotinyl-L-lysyl-[protein] = ADP + H(+) + N(6)-carboxybiotinyl-L-lysyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13501"/>
            <dbReference type="Rhea" id="RHEA-COMP:10505"/>
            <dbReference type="Rhea" id="RHEA-COMP:10506"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83144"/>
            <dbReference type="ChEBI" id="CHEBI:83145"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.14"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Acetyl-CoA carboxylase is a heterohexamer of biotin carboxyl carrier protein, biotin carboxylase and the two subunits of carboxyl transferase in a 2:2 complex.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.14" evidence="5"/>
    <dbReference type="EMBL" id="CBAR010000240">
        <property type="protein sequence ID" value="CCZ84493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00392"/>
    <dbReference type="EnsemblBacteria" id="CCZ84493">
        <property type="protein sequence ID" value="CCZ84493"/>
        <property type="gene ID" value="BN566_00392"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00655">
        <property type="reaction ID" value="UER00711"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003989">
        <property type="term" value="F:acetyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004075">
        <property type="term" value="F:biotin carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001295">
        <property type="term" value="P:malonyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004549">
        <property type="entry name" value="Acetyl_CoA_COase_biotin_COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CbamoylP_synth_lsu-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="SSF51246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00514">
        <property type="entry name" value="accC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0092">Biotin</keyword>
    <keyword evidence="5" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="1 5" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="2 4 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="7">
        <location>
            <begin position="2"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="6">
        <location>
            <begin position="121"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU365063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ84493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="454" mass="50327" checksum="507AE3828A69EDF1" modified="2013-07-24" version="1">MRFDTVLIANRGEIAVRIIRACREQNLGCVSVYSTADRDALHVKFADRAVCIGAPATADSYLRMDSIIEACRQTGATAVHPGFGFLSENAEFAKLCRENDITFIGPSPECIAMLGDKAQAKETMKAAGVPVIPGSEGALHSIEEAKEIAKKIGYPVLVKASAGGGGRGIRRVDSEEQLEEQLHVAQEEAKKFFGDDTVYLEKLIVGPHHVEVQIIADEYGNTVALGERDCSMQRRNQKVLEESPSPLMTPELRDAMQKAAVAAAKACGYCNAGTIEFLVDHERNFYFMEMNTRIQVEHPVTEWVTGIDLVQTQLRIAQGEPLPFTQEDITLRGHAIECRINAENPDCNFRPSPGTIQALHLPGGMGVRIDSAIYQGYTIPPYYDSMIAKLIVHAPTRKEAIQKMRWALSEFLVDGVETNIDFQLRLLKTEAFEQGTYDNAFLMHWLEDSKHKQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1T0</accession>
    <name>R5V1T0_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glutamate--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Glutamyl-tRNA synthetase</fullName>
            <shortName evidence="8">GluRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">gltX</name>
        <name evidence="12" type="ORF">BN566_00019</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu)</text>
            <dbReference type="Rhea" id="RHEA:23540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9663"/>
            <dbReference type="Rhea" id="RHEA-COMP:9680"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.17"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.17" evidence="8"/>
    <dbReference type="EMBL" id="CBAR010000197">
        <property type="protein sequence ID" value="CCZ84250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00019"/>
    <dbReference type="EnsemblBacteria" id="CCZ84250">
        <property type="protein sequence ID" value="CCZ84250"/>
        <property type="gene ID" value="BN566_00019"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004818">
        <property type="term" value="F:glutamate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006424">
        <property type="term" value="P:glutamyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00808">
        <property type="entry name" value="GluRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00022">
        <property type="entry name" value="Glu_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045462">
        <property type="entry name" value="aa-tRNA-synth_I_cd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020751">
        <property type="entry name" value="aa-tRNA-synth_I_codon-bd_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008925">
        <property type="entry name" value="aa_tRNA-synth_I_cd-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004527">
        <property type="entry name" value="Glu-tRNA-ligase_bac/mito"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033910">
        <property type="entry name" value="GluRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19269">
        <property type="entry name" value="Anticodon_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48163">
        <property type="entry name" value="SSF48163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00464">
        <property type="entry name" value="gltX_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="tRNA-synt_1c" evidence="10">
        <location>
            <begin position="5"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="Anticodon_2" evidence="11">
        <location>
            <begin position="335"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="8">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="8">
        <location>
            <begin position="252"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF19269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CCZ84250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="483" mass="54592" checksum="573667D806A1D3A3" modified="2013-07-24" version="1">MEKRVRTRFAPSPTGYMHIGNLRTALYAYLLAKKYDGDFILRIEDTDQERYVEGAVDIIYNTLRIAGLKWDEGPDIGGPVGPYVQSERMGMFKGYAEELVKSGHAYYCFCGKERLEEVRKIQEASHIAPMYDRHCRNLTPEEIQEKLDAGVPYVIRQKMPLDGTTTFHDEVYGDITVENSTLDDQILIKTDGMPTYNFANVVDDHLMGITHVIRGNEYLSSAPKYNLLYQAFGWDVPVYVHCSPVMKDKTHKLSKRNGDASFQDLLEKGYLPEAIINFIALLGWAPTGEQEIFTMDELIQAFDISGISKSPALFDPVKLKAINGEYIRKMTPEAFAEKAIPYIRQVVHSETADLQLLCHVLQPRTELFTDIPEQVDFIDALPDYDVELYRHKKMKTTPETAKEALTEILPVLEGISDWTVDSIHAAVFARIGELGVKNGWMLWPLRTALSGKPFTPGGGIELCAILGKDETLKRVQDGINRLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1Y1</accession>
    <name>R5V1Y1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00228</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000212">
        <property type="protein sequence ID" value="CCZ84315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00228"/>
    <dbReference type="EnsemblBacteria" id="CCZ84315">
        <property type="protein sequence ID" value="CCZ84315"/>
        <property type="gene ID" value="BN566_00228"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010697">
        <property type="entry name" value="YspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06908">
        <property type="entry name" value="YpsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="235" mass="27127" checksum="14DB14B78D16A61F" modified="2013-07-24" version="1">MEPVAFVLNADYPQRTTFLQFPGKTACFSGHREDALPLGETVVGYAATDLLKTLLAGCIEQAVQDGYRYFLDGLAEGVDLWAADDLIYRKQHGTEVLHLIAVEPCLHYLESRRSGREAMLHYVEHCEAVVTMPYQGKFSFLRRNDYMLEHSWRLICVIHKTSGGTAYTLKRAIRERKEIACISMENRDLLFQFAMHFLETGEKVPQTAAERFTYYHGHPERLRLCQSLLKRYAKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>R5V245</accession>
    <name>R5V245_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Large-conductance mechanosensitive channel</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">mscL</name>
        <name evidence="11" type="ORF">BN566_00297</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homopentamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the MscL family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000222">
        <property type="protein sequence ID" value="CCZ84390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00297"/>
    <dbReference type="EnsemblBacteria" id="CCZ84390">
        <property type="protein sequence ID" value="CCZ84390"/>
        <property type="gene ID" value="BN566_00297"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00115">
        <property type="entry name" value="MscL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019823">
        <property type="entry name" value="Mechanosensitive_channel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001185">
        <property type="entry name" value="MS_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037673">
        <property type="entry name" value="MSC/AndL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036019">
        <property type="entry name" value="MscL_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30266">
        <property type="entry name" value="PTHR30266"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01741">
        <property type="entry name" value="MscL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01264">
        <property type="entry name" value="MECHCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81330">
        <property type="entry name" value="SSF81330"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00220">
        <property type="entry name" value="mscL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01327">
        <property type="entry name" value="MSCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 10" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="83"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ84390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="156" mass="17322" checksum="149A5917E034F84C" modified="2013-07-24" version="1">MATETKDPQKKSNAITRFLGEFKEFISKGNVLDMAVGVVVGSAFTAIVNALVDNVLMPLVGAVIAGINFQDLGFHIPWGNHPYINIGAFLSAVITFLITAFCVFLIVKAVNTMRNLTFKKKEEEKKEEPEKVAPDIALLTEIRDLLKQQQNEETKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5V2G5</accession>
    <name>R5V2G5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Acetyl-CoA carboxylase biotin carboxyl carrier protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00389</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000240">
        <property type="protein sequence ID" value="CCZ84490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00389"/>
    <dbReference type="EnsemblBacteria" id="CCZ84490">
        <property type="protein sequence ID" value="CCZ84490"/>
        <property type="gene ID" value="BN566_00389"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="92" mass="9700" checksum="7D061460FC663014" modified="2013-07-24" version="1">MENNHMNLEFIQKLADLVADKNLGEITVQNGDSTITVKGKKAAAQTVVESLPVAAAAAPLAAAPAVAAPAXXLRLWLHLLQKPKQLLRLASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5V412</accession>
    <name>R5V412_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00659</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000012">
        <property type="protein sequence ID" value="CCZ82854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82854">
        <property type="protein sequence ID" value="CCZ82854"/>
        <property type="gene ID" value="BN566_00659"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="173"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="353"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="353"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="399"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ82854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="455" mass="49674" checksum="FE8280D6AAD8E30D" modified="2013-07-24" version="1">MKKKKSLSILGIVAAVLLLIVVLITLTLNVLFSQHDTPKIFGNYVYLMESDSMEVKPSTDGSTTIDASASIHAKTAVICKAYDGSEPLSKNNAILCVLAPEDTSSDKDKEGMAIRRILNIASDEETGIMKYYPTTMQVDTIGTEPAITVDSVRGICRYESAQLYSFIQFATGFSGIIVLLALPCVLLIALLIVALVRRSANKREDDAYAFEESYDELPGEEDYNYDDEDYEDEDFVNDVPQTTYQTRSGNTGTYNTGSYNMDSSYPGYGNTAVQNAQTSSFETKKSSISQNFERKAVNPNSAFQKAKTMQFQAQKNVPIPEQPYESVASQQPETNGYVGKYGADAPVAEPVQEAPQQEQTYHGAHEAPSGSSSTGSKTFLSSTGTFKRPNVDDIFGNDPFATRSTSTSRTPQRSTTAKPSAARPAAKPASRRPSSYDTASVDDLVAMIENQKKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>R5V623</accession>
    <name>R5V623_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Stage 0 sporulation protein A homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN566_01318</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000123">
        <property type="protein sequence ID" value="CCZ83549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01318"/>
    <dbReference type="EnsemblBacteria" id="CCZ83549">
        <property type="protein sequence ID" value="CCZ83549"/>
        <property type="gene ID" value="BN566_01318"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Response regulatory" evidence="9">
        <location>
            <begin position="2"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="10">
        <location>
            <begin position="124"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="8">
        <location>
            <begin position="124"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="7">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ83549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="225" mass="25626" checksum="81E53690213FB7D0" modified="2013-07-24" version="1">MNLLIVEDEVQLADALAEILQRNKYHVDTVYNGEDGLHYASTGIYDCIVLDIMLPILDGISVLRMLRKQKISTPVLLLTAKSDVADKINGLDSGADDYLTKPFVTGELLARIRALTRRTGEVAVDGLQFHNLRLDSQNYTLSYGQEQIKLSLKEYQIMEIFMSNPKQIVSKEFFIEKIWGYESDVEYNNIEVYISFLRKKLQSIHANISIKTARGIGYFLEEQPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5V638</accession>
    <name>R5V638_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">HAD-superfamily hydrolase subfamily IIB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01342</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000131">
        <property type="protein sequence ID" value="CCZ83574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01342"/>
    <dbReference type="EnsemblBacteria" id="CCZ83574">
        <property type="protein sequence ID" value="CCZ83574"/>
        <property type="gene ID" value="BN566_01342"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000150">
        <property type="entry name" value="Cof"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00099">
        <property type="entry name" value="Cof-subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="269" mass="30305" checksum="148E1B04C0F08054" modified="2013-07-24" version="1">MKNDVWLFSDLDGTLLPSDKQIPDDLRDSIQQFVQQGGHFTIATGRTIESARTYWEQLSVKEPMILFNGAMIYDPVSEQILYQQPLCASAREMAAAVLKEFPQAGSEILCADGTYVIQNTIYEQQHVAICQVTPKYCTLETMPDIPWMKILFAVDPSDMPRLQAFIQAQNWTDVDFIQSSDSFYEMLPKQVSKGSALVEYRKRFHLESPSIVVAAGDYENDRAMLEAADYGVAPANAQPAIQAIADHVLTHTCDQKAILELLLWIQSKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5V658</accession>
    <name>R5V658_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01362</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000144">
        <property type="protein sequence ID" value="CCZ83599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01362"/>
    <dbReference type="EnsemblBacteria" id="CCZ83599">
        <property type="protein sequence ID" value="CCZ83599"/>
        <property type="gene ID" value="BN566_01362"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="371" mass="39141" checksum="4F0C0B255703BA72" modified="2013-07-24" version="1">MKKKSPKVYKVKTGNTKSSSVHNVLSAVVMIAAAGLVGFVGYSIAKPFIQDDAATSDSSLQMNAAVTTQATEAATTSETTTATTAVPAAAGSTVELKTTPETTATSVVWATTTGVSTDIQMAGTAFTHESTTQATATASGSDETTGTAANETQTTTTEAVKKEGVPLANFSDAKSAQWLDRSVLESADTFTAALEQVSGTKAVVVPMKLQGGWLNYASTVSYVAGTYICDGTMTASEIANLAAEKGFSVYALISVTNDNQFPELYKDASIPLIDGSGRWLDNSVEKGGKPWMTPYSETTVSYMTAIIQELDNAGFSAAICQDFVYPSFWESDRDFIDVDAYFGADRYKAMQDLADAMVNASKNMTVVLDMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>R5V6H6</accession>
    <name>R5V6H6_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ABC transmembrane type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_01464</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000158">
        <property type="protein sequence ID" value="CCZ83729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01464"/>
    <dbReference type="EnsemblBacteria" id="CCZ83729">
        <property type="protein sequence ID" value="CCZ83729"/>
        <property type="gene ID" value="BN566_01464"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="75"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="108"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="156"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="202"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="261"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="71"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ83729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="293" mass="32872" checksum="E0299F30F4690FDF" modified="2013-07-24" version="1">MKTKKVASAKDRTFLIITIPIVALFFLFNTLPLITGAFYSFTNYRGYGDFDFVGFRNYMDLFTDSRVGHSYLFTFKYAIVGTILINVLSLLLAMGLNSKIRCKSLLRGMYFVPNILGGLIIGYIFSFIFTYIIPAVAKAMHIGFLENSILASEKHAWIGVLVVGVWQAVAMNTIIYISGLQTIPTDIYEAGDIDGANPWQKFWKLTLPMLMPFVSINLVLSTKNMLMVFDQIMSLTKGGPAQSTESISYLIYRNGMDGGQFGYQCANAVIFFIVIVAFSVIQLKMTNKKEEQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5V793</accession>
    <name>R5V793_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Stage III sporulation protein AB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01693</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000177">
        <property type="protein sequence ID" value="CCZ83964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01693"/>
    <dbReference type="EnsemblBacteria" id="CCZ83964">
        <property type="protein sequence ID" value="CCZ83964"/>
        <property type="gene ID" value="BN566_01693"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014198">
        <property type="entry name" value="Spore_III_AB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09548">
        <property type="entry name" value="Spore_III_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="118"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="166" mass="18984" checksum="490F47E04A4E1A7F" modified="2013-07-24" version="1">MLKGIGLLCLVLCGGICGFLAANRIRQKQEQLVHLAAFLHELEIRMWCHGGTLQELFQQLSEQETYQTFSFLSNTLIAMQQVSFTEAWKTSVQADKELSPDSQQMLCELGEELGISDLYTQKEILERYRSRMQQMEAEQRETSRKKIRLYQSMGLLAGMAVAILLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7G5</accession>
    <name>R5V7G5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative SAM-dependent methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00174</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000179">
        <property type="protein sequence ID" value="CCZ84014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00174"/>
    <dbReference type="EnsemblBacteria" id="CCZ84014">
        <property type="protein sequence ID" value="CCZ84014"/>
        <property type="gene ID" value="BN566_00174"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006342">
        <property type="entry name" value="FkbM_mtfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05050">
        <property type="entry name" value="Methyltransf_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01444">
        <property type="entry name" value="fkbM_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_21" evidence="1">
        <location>
            <begin position="125"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="308" mass="34624" checksum="D73EA58B537B31B4" modified="2013-07-24" version="1">MLNQLKNRYRQWQYRRICRTLQLPPQLIQDLPTLANQTQQSGAAVQQLQTTMQQTLSRLQQDDLTTLSLLERVTKLERAASSQGSHNMDLNMLMKTTYSQSGEDAILAYLFAVLGIPFAKCTYLDLGANRPKEMSNTYFFYEQGATGTLIEANPALIPALQKERSGDVILNRCIAPKSGDVIPFHVMNVDGLSTPEDVTELLANHPDLKLLETVNVETVSVNDLFAQMHAVPVLVNIDIEGMEMEILRSIDFAKYRPLVLILEMIPYSKKLPVGQKNPEILAFLQEKGYVEYAFTGINSIFIDAAQVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7K0</accession>
    <name>R5V7K0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01795</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000186">
        <property type="protein sequence ID" value="CCZ84069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84069">
        <property type="protein sequence ID" value="CCZ84069"/>
        <property type="gene ID" value="BN566_01795"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="175" mass="20666" checksum="DA1059C321E158BE" modified="2013-07-24" version="1">MSHLNLTVELAEKSNFCNRYLELCSRYSESLLMDTTDLRQRYPRSLANITKKVVDTKLVKSILNELGYPYKYFGGEKFYRLYLLKKEKYEVWFHLELYKNSIFDGHLDPHVYIIYNEEYFAGGTFPCIFDSSGGIKPTKYMLRPAYTGEVLPELIKELIDLAFSYVREFEKLKEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7Q2</accession>
    <name>R5V7Q2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01859</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0473 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000191">
        <property type="protein sequence ID" value="CCZ84134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01859"/>
    <dbReference type="EnsemblBacteria" id="CCZ84134">
        <property type="protein sequence ID" value="CCZ84134"/>
        <property type="gene ID" value="BN566_01859"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009711">
        <property type="entry name" value="UPF0473"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06949">
        <property type="entry name" value="DUF1292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="112" mass="13150" checksum="27106AB71E6BFFCE" modified="2013-07-24" version="1">MSEERNEFTPDLFTLEDEDGNEQVFELLDVLEYEDNTYYALVPYYGDDEDALAQDDGEFVILKTEEVNGEEMLVSIEEDAEYETIGNKFLERLNEIYGDEDDEDEDDDEIFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7R5</accession>
    <name>R5V7R5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uridine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01874</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000192">
        <property type="protein sequence ID" value="CCZ84149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01874"/>
    <dbReference type="EnsemblBacteria" id="CCZ84149">
        <property type="protein sequence ID" value="CCZ84149"/>
        <property type="gene ID" value="BN566_01874"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006083">
        <property type="entry name" value="PRK/URK"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00485">
        <property type="entry name" value="PRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PRK" evidence="1">
        <location>
            <begin position="48"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="311" mass="35849" checksum="E344ACE7BA4D4D62" modified="2013-07-24" version="1">MQTIFLSDLKAALLEHPQAWITQMEQQHWAQLRTIAEEIAEHRRTCPIILLSGPSGSGKTITAMMLESILDGMGLETHTLSMDHYFKSITQEQLKLAEIGEFDLESPERVDIPFLNQQLKAIRDCEPVELPRYDFRISSRVPSGRILQRKPEELVILEGIHALNPSVITLSDHDAARCYVNVQTALRTDHDQLIPPTYVRLLRRMIRDLKYRKRSLTETIRMHHTVQVGEDTFITPYRFLAQYEVNTFLPYETFAYTAVLEQIPEAVSIARTIPELKAILEEQVPFSLDWIPETALVREFIGGSVYYQESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7S8</accession>
    <name>R5V7S8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ABC transporter substrate-binding protein family 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01889</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000193">
        <property type="protein sequence ID" value="CCZ84164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01889"/>
    <dbReference type="EnsemblBacteria" id="CCZ84164">
        <property type="protein sequence ID" value="CCZ84164"/>
        <property type="gene ID" value="BN566_01889"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030678">
        <property type="entry name" value="Peptide/Ni-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000914">
        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PTHR30290"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002741">
        <property type="entry name" value="MppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004394742" evidence="1">
        <location>
            <begin position="28"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="domain" description="SBP_bac_5" evidence="2">
        <location>
            <begin position="79"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="555" mass="62938" checksum="B5C98A7BE0EF5610" modified="2013-07-24" version="1">MNHLLKKCLCLCLPVVLLSGCTSPFQKEESDGSGYLFTYTLLGNPENLDPLLATDASSKLILRNMMQGLLEENPDGTLSNGVADSYTMQDDGLLYTFHLRDDCYWYFDENQNEQIDDGETWKVTAQDFVYAFQRMFLAETRSPYRETFSCLLNATEIMQGTVDASQIGVTAQDDQTLIFQLAESNSRFPELLATTAALPCNETFFQQTKGRYGLDETSVISNGSFYLRKWFYDPYGSDNVIYMQCNSANDSKEADRKIYPSDLTFLIRKKQAQAEEAFDSEQSDILTTSIYPKQELAQPDVYTVNSYSSMTLGLVFNPTNTAYANTNIRKALSESIDRSNIGADSDGDLTGASGIVPPDTRVDAVRYRDNVPEPSTTYQNETAKQDFEKGLQELKMDSLPSDQILVCPELMDCSYLHEILQNWQKTFEFYIGIEEVTASEYQDRLESGNYGIALYGVTGSENNPASVLSAFCSDQNPFYQNESFDAMIQQTTTCNNAAQLTDLCMQMENQLLSEYSFIPVYYKKTYLVYRNENRDIFYDPYSGIIDFRIVKYFGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7V0</accession>
    <name>R5V7V0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Fe-S iron-sulfur cluster assembly protein NifU family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01919</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01919"/>
    <dbReference type="EnsemblBacteria" id="CCZ84194">
        <property type="protein sequence ID" value="CCZ84194"/>
        <property type="gene ID" value="BN566_01919"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06664">
        <property type="entry name" value="IscU_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017787">
        <property type="entry name" value="NIF_FeS_clus_asmbl_NifU-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002871">
        <property type="entry name" value="NIF_FeS_clus_asmbl_NifU_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10093">
        <property type="entry name" value="PTHR10093"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01592">
        <property type="entry name" value="NifU_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03419">
        <property type="entry name" value="NifU_clost"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NifU_N" evidence="1">
        <location>
            <begin position="4"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="143" mass="15357" checksum="B6AB59A018AF31E1" modified="2013-07-24" version="1">MALYSEKVMDHFLHPRNVGVLEDADGVGEVGNAKCGDIMKIYLKIDNDVISDVKFETFGCGSAIASSSMATEMIKGKPVSEALQLTNKAVAEALDGLPAHKLHCSVLAEEAIKSALKDYYDKNGIAYDAAQFPNCEDCAHCAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7X7</accession>
    <name>R5V7X7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Oxidoreductase aldo/keto reductase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01954</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01954"/>
    <dbReference type="EnsemblBacteria" id="CCZ84229">
        <property type="protein sequence ID" value="CCZ84229"/>
        <property type="gene ID" value="BN566_01954"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NADP_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="SSF51430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="143" mass="16425" checksum="E62B11E254261034" modified="2013-07-24" version="1">MYFCSQKCFCTQHFYQVIFKINILIRINIDEKTDSALLDFCKNEQIQVEVYFPIAHREALKNSVITKMAAKYGVSAAQLCIRYGLQLGTIALPKTANPEHMKNNADIDFVITDIDFQTLLQMEELRDYGAYNIFPVFSGKPLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>R5V8F4</accession>
    <name>R5V8F4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Alpha-1,4 glucan phosphorylase</fullName>
            <ecNumber evidence="10">2.4.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN566_00306</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Allosteric enzyme that catalyzes the rate-limiting step in glycogen catabolism, the phosphorolytic cleavage of glycogen to produce glucose-1-phosphate, and plays a central role in maintaining cellular and organismal glucose homeostasis.</text>
    </comment>
    <comment type="function">
        <text evidence="8">Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>[(1-&gt;4)-alpha-D-glucosyl](n) + phosphate = [(1-&gt;4)-alpha-D-glucosyl](n-1) + alpha-D-glucose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:41732"/>
            <dbReference type="Rhea" id="RHEA-COMP:9584"/>
            <dbReference type="Rhea" id="RHEA-COMP:9586"/>
            <dbReference type="ChEBI" id="CHEBI:15444"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="EC" id="2.4.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 10">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the glycogen phosphorylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.1" evidence="10"/>
    <dbReference type="EMBL" id="CBAR010000224">
        <property type="protein sequence ID" value="CCZ84399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00306"/>
    <dbReference type="EnsemblBacteria" id="CCZ84399">
        <property type="protein sequence ID" value="CCZ84399"/>
        <property type="gene ID" value="BN566_00306"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008184">
        <property type="term" value="F:glycogen phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102250">
        <property type="term" value="F:linear malto-oligosaccharide phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102499">
        <property type="term" value="F:SHG alpha-glucan phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011833">
        <property type="entry name" value="Glycg_phsphrylas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000811">
        <property type="entry name" value="Glyco_trans_35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035090">
        <property type="entry name" value="Pyridoxal_P_attach_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11468">
        <property type="entry name" value="PTHR11468"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00343">
        <property type="entry name" value="Phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000460">
        <property type="entry name" value="Pprylas_GlgP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02093">
        <property type="entry name" value="P_ylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00102">
        <property type="entry name" value="PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6 9 10" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="9">
        <location>
            <position position="628"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000460-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ84399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="785" mass="88324" checksum="230107ECCA056707" modified="2013-07-24" version="1">MQKDQLKEQLTQALAGIAPKNATPQQLHQAIGDVVMHNIAENWSKSTQAHETNRHACYFSMEFLVGRAVFNNLLCLGCYKEVETALQEMGASLASLEEIEDAALGNGGLGRLAACFLDSAATLNLPLDGYGIRYKYGLFKQSIVDGFQKEEPDNWMQYGDAWSVRCEKDAVLVHFNGQTVKAVPYDMPVIGCKTKHIGTLRLWQAEPVQTFDFDLFNQQKYLEAAAETVYAEDISRVLYPNDDTWDGKKLRLKQQYFFCAASLSDILKKHKATYGTLDNLADKLAVQLNDTHPVISIPELIRLLMLDDSKRTFADAFAIAQKVFRYTNHTVMPEALEKWDCHLVEELLPELYPIIVQINEAMLTELYQKNIPLEQIAKMKIIQNGMVHMAHLAVYASSYTNGVAAIHTQILKDTVLADWYKVWPERFLNETNGITQRRWLALCNQELSGLLTELAGSDGWMTDLKQLKALETKGSPEVLERFCSIKQTKKQQLADFIAAKEDVTIDPNSIFDIQIKRLHEYKRQLLNAFSILYLYEGIKDGSIQNFTPTTFLFGAKSAPGYRRAKAIIKFINEVGKLIANDPDVNQLIKVVFVSNYNVSYAEKLVAAADVSEQISTAGTEASGTGNMKLMLNGAVTLGTYDGANVEIVEEAGEDCNYIFGAKVEELETILPQYDSRKIFSENPKIRRVVERLIDGTLSDGGSGDFRELYYALLDGASWHAPDHYYLLGDLESYVAAKLRCNQDYQNRMDFAAKQWKNICNAGKFSSDRTIAGYAKDIWQIDPVTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>R5V935</accession>
    <name>R5V935_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">Glutamate dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN566_00467</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4 8">Belongs to the Glu/Leu/Phe/Val dehydrogenases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000257">
        <property type="protein sequence ID" value="CCZ84614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00467"/>
    <dbReference type="EnsemblBacteria" id="CCZ84614">
        <property type="protein sequence ID" value="CCZ84614"/>
        <property type="gene ID" value="BN566_00467"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004352">
        <property type="term" value="F:glutamate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:cellular amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05313">
        <property type="entry name" value="NAD_bind_2_Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminiacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006095">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033524">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006096">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006097">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_dimer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014362">
        <property type="entry name" value="Glu_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033922">
        <property type="entry name" value="NAD_bind_Glu_DH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00208">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02812">
        <property type="entry name" value="ELFV_dehydrog_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000185">
        <property type="entry name" value="Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00082">
        <property type="entry name" value="GLFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00839">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="SSF53223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00074">
        <property type="entry name" value="GLFV_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ELFV_dehydrog" evidence="9">
        <location>
            <begin position="207"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="383"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalysis" evidence="7">
        <location>
            <position position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ84614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="452" mass="49017" checksum="0CCAA1CD7627E2D7" modified="2013-07-24" version="1">MVFTFKNQYLQGVYDKTAKCYANEPEFLQAVGEVLQSLEPVVAKDPSYETNGVIDRIVEPERMISFRVSWVDDNGKVQVNHGYRCQFNSAIGPYKGGLRLHPSVCPSVIKFLGFEQTFKNSLTTLPMGGGKGGSDFDPKGKSDGEVMRFCQSFMTELCKHIGADTDVPAGDIGTGAREIGYMFGQYKRLRNEFTGVLTGKALSYGGSLARTEATGYGLLYFTEEMMSVMKNDTVKGKTIVVSGSGNVAIYATEKATQLGGKVVALSDSNGYIYDPNGINLDVVKQIKEVERGRIKEYVNRVEGATYTEGCKGIWTIKCDIALPCATQNEIDEESAKILVANGTKVVAEGANMPSTPEAIAVFQKSGLLFGPAKAANAGGVATSGLEMSQNSERLSWTFEEVDAKLKGIMINIFHNAYDRSKEYGVEGDLVVGANIAGFLKVADAMKWQGVAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5V989</accession>
    <name>R5V989_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Flavin_Reduct domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00532</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000267">
        <property type="protein sequence ID" value="CCZ84679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00532"/>
    <dbReference type="EnsemblBacteria" id="CCZ84679">
        <property type="protein sequence ID" value="CCZ84679"/>
        <property type="gene ID" value="BN566_00532"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016646">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-NH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002563">
        <property type="entry name" value="Flavin_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01613">
        <property type="entry name" value="Flavin_Reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavin_Reduct" evidence="1">
        <location>
            <begin position="24"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="170" mass="19483" checksum="23AD1105BBE0E0AB" modified="2013-07-24" version="1">MQFEQKTLSEIQWNPFDMIGKQWYLLTAGGLSDYNTMTASWGFMGEMWGKPSFLCGVRTNRHTLPFMEQNDCFSVSFFEESQRAALQFCGTHSGRDCDKAKETGLTPVELDGVATFAEAKLVLICKKQYTDMLKPEGFLIPETYQRWYNTDPLHKLFVGEILAAYERTDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>R5V9A3</accession>
    <name>R5V9A3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Uridine phosphorylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_00552</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PNP/UDP phosphorylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000271">
        <property type="protein sequence ID" value="CCZ84699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00552"/>
    <dbReference type="EnsemblBacteria" id="CCZ84699">
        <property type="protein sequence ID" value="CCZ84699"/>
        <property type="gene ID" value="BN566_00552"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016763">
        <property type="term" value="F:pentosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009164">
        <property type="term" value="P:nucleoside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018016">
        <property type="entry name" value="Nucleoside_phosphorylase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="SSF53167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01232">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PNP_UDP_1" evidence="5">
        <location>
            <begin position="18"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ84699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="257" mass="27809" checksum="D323334E7854D482" modified="2013-07-24" version="1">MQATIPFHLQCDPKKIARYVLLPGDPGRVPKIAAYLEQAVLVSQNREFTIYSGLLDGVPVSVASTGIGGASAAIAMEELVQCGADTFLRVGTCGGMQPELIPGTLILPNGAVRMEGTSREYMPIAFPAVPDFSLLQHLAAAADSLQYSYQIGVVQCKDSFYGQHDPERMPVKQELLSNWAAWKAGGVLASEMESAALFVTAAVLHVRCATVLHMIWNQEREEVSAETAQLDPDTSIRVAIAALRMQIAADRKTEEKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>R5VHB3</accession>
    <name>R5VHB3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">RNA polymerase sigma factor sigma-70 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN566_00736</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000029">
        <property type="protein sequence ID" value="CCZ82936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00736"/>
    <dbReference type="EnsemblBacteria" id="CCZ82936">
        <property type="protein sequence ID" value="CCZ82936"/>
        <property type="gene ID" value="BN566_00736"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000943">
        <property type="entry name" value="RNA_pol_sigma70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007624">
        <property type="entry name" value="RNA_pol_sigma70_r3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007630">
        <property type="entry name" value="RNA_pol_sigma70_r4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04539">
        <property type="entry name" value="Sigma70_r3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04545">
        <property type="entry name" value="Sigma70_r4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00046">
        <property type="entry name" value="SIGMA70FCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="7">
        <location>
            <begin position="7"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r3" evidence="6">
        <location>
            <begin position="84"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4" evidence="8">
        <location>
            <begin position="161"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ82936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="214" mass="23961" checksum="376B16A03867B24C" modified="2013-07-24" version="1">MNAPTAEEHLGLVHLCANRFRGRGMEYEDLYSAGCIGLLKAVKAFDCTRGVQFSTYAVPVILGEIKRLFRDGGTVKVSRSLKEHAMQIQQLQEQFQQKHGRAATLTELAERSGLSEEVIAESLCAAQPPLSLTTAAEEAPLEIPVPAPDTELENLFALRQVLQILPQADRKLLQYRYFQNLTQTETAKRLGISQVQVSRKEKKILSRLRQELLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5VHI8</accession>
    <name>R5VHI8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_00784</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000036">
        <property type="protein sequence ID" value="CCZ82991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00784"/>
    <dbReference type="EnsemblBacteria" id="CCZ82991">
        <property type="protein sequence ID" value="CCZ82991"/>
        <property type="gene ID" value="BN566_00784"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="6"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ82991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="233" mass="25868" checksum="F55481500E4EFD13" modified="2013-07-24" version="1">MATSALQFSHVTLGYENIITTSDLNLTIPQGDYLCIIGENGSGKSTFVKNLLGLLKPLSGEITLTGDWKRSDIGYLPQQTPAQRDFPASVREIVRSGFLNQMKHRPFYTAAEKNAAQQAMEKLGILPLQNRCYRELSGGQQQRVLLARALCAAQKLLILDEPTTGLDPVASEELYQVLQHLNCTESITIIMVTHDVERAVYYAKHILHLGQKTYTYDTAEAYRHAAASRQGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5VHU4</accession>
    <name>R5VHU4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Metallo-beta-lactamase superfamily protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00874</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000051">
        <property type="protein sequence ID" value="CCZ83086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00874"/>
    <dbReference type="EnsemblBacteria" id="CCZ83086">
        <property type="protein sequence ID" value="CCZ83086"/>
        <property type="gene ID" value="BN566_00874"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07731">
        <property type="entry name" value="ComA-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035681">
        <property type="entry name" value="ComA-like_MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="Lactamase_B" evidence="2">
        <location>
            <begin position="65"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="322" mass="34389" checksum="D3B2710EA7BE3554" modified="2013-07-24" version="1">MAATQKSKQPAAKNGKQKANQQKEKIIGLIALIVVAFAGGAKVFGCAPAVDSNASATVSFLDVGQGDSVLIQAAGKTVLIDGGERDQGSVVLDDLKKYGVTTIDYMIASHPHSDHIGGLVDVLEDAAVSSDLTVSMVLLPEVPDKDVPTTRVYEAFLDGIEDNHIDPTFVSEKQTLNLGGSTTLELIAPPAGKTYSSLNDYSMAAYLNCGGKTFLFTGDAEKDEEADWLESGVFDGMHADVFKAGHHGSRTSSSEALLEQIQPTYAVISCGTDNSYGHPHEEALDRLGVYCDKIYRTDLDGTVICKVEDGELKWSFDKEYAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>R5VHZ0</accession>
    <name>R5VHZ0_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribosomal RNA small subunit methyltransferase G</fullName>
            <ecNumber evidence="2">2.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">16S rRNA 7-methylguanosine methyltransferase</fullName>
            <shortName evidence="2">16S rRNA m7G methyltransferase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rsmG</name>
        <name evidence="3" type="ORF">BN566_00944</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Specifically methylates the N7 position of a guanine in 16S rRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily. RNA methyltransferase RsmG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CBAR010000069">
        <property type="protein sequence ID" value="CCZ83161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00944"/>
    <dbReference type="EnsemblBacteria" id="CCZ83161">
        <property type="protein sequence ID" value="CCZ83161"/>
        <property type="gene ID" value="BN566_00944"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070043">
        <property type="term" value="F:rRNA (guanine-N7-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00074">
        <property type="entry name" value="16SrRNA_methyltr_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003682">
        <property type="entry name" value="rRNA_ssu_MeTfrase_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31760">
        <property type="entry name" value="PTHR31760"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02527">
        <property type="entry name" value="GidB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003078">
        <property type="entry name" value="GidB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00138">
        <property type="entry name" value="rsmG_gidB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="2">
        <location>
            <begin position="127"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="235" mass="26935" checksum="BE0BEA1C4C4B30A3" modified="2013-07-24" version="1">MKWERAEKMFAHSGLSLTEGQFHQFSTYQEILVETNRRMNLTTITEDAEVWRKHFLDSCLPFQYVEIPKGAAVIDVGTGAGFPGIPMRILRPDLRLTMLDSLQKRVHFLESVCQALQIPVHCVHARAEFGGKQPDLREQFDVACARAVAAMPLLCEYCLPFVKVGGMFLALKGPNETVEDAMEHMKRLGGELEETFLYSLEQNEARRLFVIRKVRETPKQYPRSPKQLQNRKHSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5VI16</accession>
    <name>R5VI16_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">30S ribosomal protein S6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpsF</name>
        <name evidence="6" type="ORF">BN566_00979</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Binds together with S18 to 16S ribosomal RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the bacterial ribosomal protein bS6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000070">
        <property type="protein sequence ID" value="CCZ83196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00979"/>
    <dbReference type="EnsemblBacteria" id="CCZ83196">
        <property type="protein sequence ID" value="CCZ83196"/>
        <property type="gene ID" value="BN566_00979"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00473">
        <property type="entry name" value="bS6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00360">
        <property type="entry name" value="Ribosomal_S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000529">
        <property type="entry name" value="Ribosomal_S6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020814">
        <property type="entry name" value="Ribosomal_S6_plastid/chlpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035980">
        <property type="entry name" value="Ribosomal_S6_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014717">
        <property type="entry name" value="Transl_elong_EF1B/ribosomal_S6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21011">
        <property type="entry name" value="PTHR21011"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01250">
        <property type="entry name" value="Ribosomal_S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54995">
        <property type="entry name" value="SSF54995"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00166">
        <property type="entry name" value="S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ83196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="97" mass="10953" checksum="C92F46BC0CFD86C6" modified="2013-07-24" version="1">MAKLNAKYELMAVYSLAKGEEVAKELVEKFKSKIEAAATLDEVNEWGKRKLAYPINDEPDGYYALYSFTATPDFPAELERVLNITDGVLRFLVTVTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>R5VI27</accession>
    <name>R5VI27_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transmembrane permease MsmF</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_00994</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000073">
        <property type="protein sequence ID" value="CCZ83211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00994"/>
    <dbReference type="EnsemblBacteria" id="CCZ83211">
        <property type="protein sequence ID" value="CCZ83211"/>
        <property type="gene ID" value="BN566_00994"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="96"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="157"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="200"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="249"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="59"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ83211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="289" mass="32180" checksum="717FE411234F23D9" modified="2013-07-24" version="1">MSFFGPALVLMTVFLFIPMILTLIYSFTDYFALNPKLTHFIGLENFKNIFKDDLFTTAFGNTIKFVLIIVPLQTLGALGLALLINKVTVCKKYFKVAFFIPVVMSLAVVSNLWMQIYSPEGILNTILSNFGIDAQPFIYGANQALPSIALMSVWQGVGYQMIIFLGGLQAINPALYEAAEMDHASAWSKFKNITLPELKPLCVFVFITVTIGAFRMIVQPMVMTGGGPSHSTYTIVYDIYETGTVNWEIGLASAMAIVFTVFVVILTIIQTLLTKDKEEKHENKKAKNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>R5VIJ4</accession>
    <name>R5VIJ4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN566_01152</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000093">
        <property type="protein sequence ID" value="CCZ83376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01152"/>
    <dbReference type="EnsemblBacteria" id="CCZ83376">
        <property type="protein sequence ID" value="CCZ83376"/>
        <property type="gene ID" value="BN566_01152"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="68"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="168"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="252"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="281"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="27"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="343"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="186"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="376"/>
            <end position="383"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ83376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="593" mass="65425" checksum="ACC552EA6A37B837" modified="2013-07-24" version="1">MKKQPSNLSRLMQYAGSHRYLTYASWILSAGSALLALVPFWYLWKIIAEVIDVMPDFSKAEHLIQNGWLAVLFAVLSFVVYVCGLMCSHLSAFRVATNLRLQMLHHLAELPLGFTQQFGSGKLRKTINESSGATETYLAHMLPDTANAIATPIGLIALLLFFDWKLGLLSLIPVAAGFLVMTSMTGENMKKKMEEYQNALDEMSNEAVEYVRGIPVVKTFGQTVFSFQRFKQSIDRYKTWTIAYTKDLRVPMMLYTTVINGVFAVLIAAALVMTRNGVTDIFLLNLLFYIIITPVISLTMTKMMFMSENGMIVADALKRVDTILDAAPLFNNAAGTVPTDASIQLQHVSYSYDGKRNALDDVSLTIPSGKTAAFVGSSGSGKTTAANLISRFFDTQKGAVLIGGVNVKEIPKEQLMETISFVFQNSRLIKGTILDNIKMGKPDATEQEVRAALEQAQCMDILEKLPNGLQTVIGTGGIYLSGGEQQRIAIARTILKNAPILIFDEATAFADPDNEAKVQAAISALAKDKTILIIAHRLSTITHADYIFVLEEGKLIESGTAEELQRKNGVFQKMWDAYQTSVQWKVSKEGQTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5VIW5</accession>
    <name>R5VIW5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phage capsid family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01230</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000109">
        <property type="protein sequence ID" value="CCZ83456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01230"/>
    <dbReference type="EnsemblBacteria" id="CCZ83456">
        <property type="protein sequence ID" value="CCZ83456"/>
        <property type="gene ID" value="BN566_01230"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="320" mass="34902" checksum="F6F5C6C10CAED0E5" modified="2013-07-24" version="1">MYQNLKLEKGLYHLTNKSFVQALEALDPSAQYADTPLAGLDAYERQLKRFDIRISGAQCDRVEKFFTSTESAVLFPEFVRRAVQQGIDASMLSDLTAVETHTDSSQYLGCTITETDAYGTVAQGAAMTSSTILEATTALTLNKYGRLVKASYEAVRRQRLDVFAVLLKSVGMQLGQALMQQAITTLTTTVGSTTTKAGSALAYSDLATLYGKFSTYDMNLLLVSPKNAAAILAMSEMEDVVPVEQGQVRLPFGTILCKVPQMSDTVILGVDKNFALEMVTGSNLILETDRLIENQLEAISVSLQCGFRVLTKNAVHKMTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJ71</accession>
    <name>R5VJ71_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sporulation protein YtfJ</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01310</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000121">
        <property type="protein sequence ID" value="CCZ83541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01310"/>
    <dbReference type="EnsemblBacteria" id="CCZ83541">
        <property type="protein sequence ID" value="CCZ83541"/>
        <property type="gene ID" value="BN566_01310"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014229">
        <property type="entry name" value="Spore_YtfJ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39162">
        <property type="entry name" value="PTHR39162"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09579">
        <property type="entry name" value="Spore_YtfJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF021377">
        <property type="entry name" value="YtfJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="145" mass="15271" checksum="A0EFC5F0579D33C7" modified="2013-07-24" version="1">MPESNSKPSKTTIPNLMDVTMEKIRDMVDVETIIGDPITVGTDVTIIPISKVSYGFASGGSDLPAKVPKDLFGGGTGAGISIQPVGFLVVQNGDVRLLQMTEGNGDTANNLIRTLPEVISKVSGLVKSKKKPNKSSDDLNTDEKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJ84</accession>
    <name>R5VJ84_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase</fullName>
            <ecNumber evidence="10">2.8.4.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN566_01329</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i(6)A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.3" evidence="10"/>
    <dbReference type="EMBL" id="CBAR010000130">
        <property type="protein sequence ID" value="CCZ83561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01329"/>
    <dbReference type="EnsemblBacteria" id="CCZ83561">
        <property type="protein sequence ID" value="CCZ83561"/>
        <property type="gene ID" value="BN566_01329"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035596">
        <property type="term" value="F:methylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005839">
        <property type="entry name" value="Methylthiotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020612">
        <property type="entry name" value="Methylthiotransferase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013848">
        <property type="entry name" value="Methylthiotransferase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038135">
        <property type="entry name" value="Methylthiotransferase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006463">
        <property type="entry name" value="MiaB_methiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01938">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00919">
        <property type="entry name" value="UPF0004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00273">
        <property type="entry name" value="(dimethylallyl)adenosine_tRNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01061">
        <property type="entry name" value="methylthiotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00089">
        <property type="entry name" value="TIGR00089"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51449">
        <property type="entry name" value="MTTASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01278">
        <property type="entry name" value="MTTASE_RADICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MTTase N-terminal" evidence="12">
        <location>
            <begin position="31"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="13">
        <location>
            <begin position="171"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="TRAM" evidence="11">
        <location>
            <begin position="404"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CCZ83561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="476" mass="54105" checksum="FC6F4AAF7B5623A1" modified="2013-07-24" version="1">MQETTNLQKTMSIQACTERVQKIVAVSYPCAKAYVHSFGCQLNVSDGEKIKGLLQSMGFSLTETHEDADVIVFNTCAVRESAEDRVYGMLGVMKKYKETHPRTILILSGCMGSEPKTLEKVRAHYSFIDILMGTASIGELPRLLATYYDTKKFVYDMDSTAMPSEQMQQVRNSGFKASVPIMYGCNNFCTYCIVPYVRGRERSRQPEVILREVQHLIQDGYKEIMLLGQNVNSYGKDLEQPISFAELLRKINAMEGEFVIRFMSSHPKDATPELLDTILSCEKVGQHLHLPVQCGSDAILSAMNRHYTVEQYLNIVDYLRERRPDFSLTSDLIVGFPNESEADFQGTLDLIQRVQYDNLYTFIYSKRSGTKAANMPDATSDAEKRQRMERLLTLQREIATKNYKRFLGRTMRVLVEGDSKRGDGWLFGKSNEAIIVEFMGNRSLIGTFVSVQITESKNWAVSGRLLDDERKEAVQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJ90</accession>
    <name>R5VJ90_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Isoleucine--tRNA ligase</fullName>
            <ecNumber evidence="11">6.1.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Isoleucyl-tRNA synthetase</fullName>
            <shortName evidence="11">IleRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ileS</name>
        <name evidence="16" type="ORF">BN566_01339</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 11">Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile)</text>
            <dbReference type="Rhea" id="RHEA:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:9666"/>
            <dbReference type="Rhea" id="RHEA-COMP:9695"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78528"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 11">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.5" evidence="11"/>
    <dbReference type="EMBL" id="CBAR010000131">
        <property type="protein sequence ID" value="CCZ83571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01339"/>
    <dbReference type="EnsemblBacteria" id="CCZ83571">
        <property type="protein sequence ID" value="CCZ83571"/>
        <property type="gene ID" value="BN566_01339"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004822">
        <property type="term" value="F:isoleucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006428">
        <property type="term" value="P:isoleucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07960">
        <property type="entry name" value="Anticodon_Ia_Ile_BEm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02002">
        <property type="entry name" value="Ile_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033708">
        <property type="entry name" value="Anticodon_Ile_BEm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002301">
        <property type="entry name" value="Ile-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023585">
        <property type="entry name" value="Ile-tRNA-ligase_type1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010663">
        <property type="entry name" value="Znf_FPG/IleRS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06827">
        <property type="entry name" value="zf-FPG_IleRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00984">
        <property type="entry name" value="TRNASYNTHILE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="SSF50677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00392">
        <property type="entry name" value="ileS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 11 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 11 12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="tRNA-synt_1" evidence="13">
        <location>
            <begin position="40"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="domain" description="Anticodon_1" evidence="15">
        <location>
            <begin position="700"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="domain" description="zf-FPG_IleRS" evidence="14">
        <location>
            <begin position="904"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="11">
        <location>
            <begin position="70"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="11">
        <location>
            <begin position="614"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="573"/>
        </location>
        <ligand>
            <name>L-isoleucyl-5'-AMP</name>
            <dbReference type="ChEBI" id="CHEBI:178002"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="617"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="907"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="910"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="927"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="930"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF06827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CCZ83571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="936" mass="106271" checksum="7ACB852A3813DA43" modified="2013-07-24" version="1">MKTNWEEPDTDMAQDYNATLNLPKTAFPMRGNLPKREPDVLGKWEEQDLYEKMVQKNQGRPMYVLHDGPPYANGEIHLGTALNKVLKDFIIKYKNMSGFCAPYVPGWDTHGLPIELKALKSIGVENGAISPVELRKHCKNFAMKHVQNQMKQFKRLGSLGHYCDPYLTLKPEYEARQVEVFGEMAKRGYMYKGLKPVYWCPDCQTALAEAEIEYENDPCDSIYVKFQVMDDKGVFSKLGIDPKQVYFVIWTTTTWTIPGNLAVCVGPDYEYTLVKAGSEYYCMAVELVAPTMKAAGITDYETIGTFMGSELEHIQTKHPLYDRNSMVIVGDHVTLESGTGCVHTAPGYGVEDFEVCKNYDEIGILVCVDAHGKQTAEAGEFEGMDTQQANKAIAAKLVECGAMLATERIVHQYPHCWRCHQPIIYRATEQWFCSVKGFKDDAIRAIESVQWIPEWGEERIKGMVRDRSDWCISRQRTWGVPIPIIYCKNCKKPIINDTTIKNIADLFRAEGADAWWTKDVKDFIDSSVTCECGCKEFTKEYDIMDVWFDSGVSHTAVMEQYDCLRWPADLYLEGADQYRGWFQSSLLTSVVYKGKAPYKAVCTHGWVVDGEGRKMSKSLGNGIMPDEIIQQYGADILRLWVASSDYHSDIRISKAILQQLSDAYKKIRNTARYILGNLGNGAGFNPDTDMVSDDQLHELDRWALIRLDRLIDKVHEGYTNFDFHVVFHSIHNFCTTDMSNFYLDIIKDRLYCEKETSVARRAAQTTMYRILRGMTLLLAPVLSFTAEEIWGFMPHGAADDTESVFLNQMPEKSGLVADDAFTEKWNLIYQTRLDVNKMLEEKRNEKVIGKSLEAAVEIAVADDAAYAILSENRELLEKVLIVSSVTVTKTDAAENQYLITTAAGEKCERCWMYSTTVGEDKTHLTLCARCAHVISE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJI0</accession>
    <name>R5VJI0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ATP-dependent Clp protease ATP-binding subunit ClpX</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_01427</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the ClpX chaperone family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000153">
        <property type="protein sequence ID" value="CCZ83691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01427"/>
    <dbReference type="EnsemblBacteria" id="CCZ83691">
        <property type="protein sequence ID" value="CCZ83691"/>
        <property type="gene ID" value="BN566_01427"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010603">
        <property type="entry name" value="Znf_CppX_C4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038366">
        <property type="entry name" value="Znf_CppX_C4_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06689">
        <property type="entry name" value="zf-C4_ClpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00994">
        <property type="entry name" value="zf-C4_ClpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51902">
        <property type="entry name" value="CLPX_ZB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="ClpX-type ZB" evidence="6">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ83691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="271" mass="29822" checksum="AFD45B4C3C6C4234" modified="2013-07-24" version="1">MSERKETCCNFCGKPESLVDSMLSAGDVHICNECVCYCYEMLYGPTPQSRAKSGKKSKSSAKQIQLKKPADMKKILDEYVIGQDAAKISLSVAVYNHYKRILSQDDGEDVELQKSNVLLLGPTGVGKTFLAQTLARILDVPFAIADATTITEAGYVGEDVENVLLRLIQAADFDVEAAERGIIYIDEIDKISRKSENTSLTRDVSGEGVQQALLKIIEGTVSNVPPQGGRKERFPLYRRRADGNIRIRNLSASIPRIFCSSAVVHLTGWKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJL9</accession>
    <name>R5VJL9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative superoxide reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01471</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000159">
        <property type="protein sequence ID" value="CCZ83736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83736">
        <property type="protein sequence ID" value="CCZ83736"/>
        <property type="gene ID" value="BN566_01471"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036073">
        <property type="entry name" value="Desulfoferrodoxin_Fe-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49367">
        <property type="entry name" value="SSF49367"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="10"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="194" mass="22036" checksum="DC6C6001A0F819D9" modified="2013-07-24" version="1">MDAQEIGTMIQRLREQQNLTQKTLAERLAVSDKTISKWETGRGLPDMALFQPLAEALQVSVSELFSGVQIVNQNRSENLKKMGFYVCPICGNVLYSMGEGVFSCCGISLPKLQAEPATDTHTRTIQQVEYDYVVSMEHPMEKTHFLSFFAYVTSDRILFVKCYPEQNPMVRFPMMGHGTIYSYCNRHGLFQVSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJM7</accession>
    <name>R5VJM7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cell envelope-related function transcriptional attenuator common domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_01481</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LytR/CpsA/Psr (LCP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000161">
        <property type="protein sequence ID" value="CCZ83746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83746">
        <property type="protein sequence ID" value="CCZ83746"/>
        <property type="gene ID" value="BN566_01481"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004474">
        <property type="entry name" value="LytR_CpsA_psr"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03816">
        <property type="entry name" value="LytR_cpsA_psr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00350">
        <property type="entry name" value="lytR_cpsA_psr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="295"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="LytR_cpsA_psr" evidence="4">
        <location>
            <begin position="357"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="10"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="41"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="64"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="601" mass="66696" checksum="FE8B40D65C8E08CF" modified="2013-07-24" version="1">MRKQRNTDAEETQFVSASEIAAKRSTDQLDTQQLDAVQTEYRDRNPYRREKQELHGTPMTPEQEAASYREQTYNSNYGNASQQPYNNGYGNASQQPYNNGYRNASQQPYNNGYGNAQQQPYNNGYGNAPQQPYNNDYGNAPQQPYNNGYGNAPQQPYNNGYGNAPQQPYNNGYGNAPQQPYNNGYSNAPQQPYNNSYSNAPQQPHYNTYNNNAPRQNYAGGQQHSGSSYRGTATTASPRPQRQQAAPPPQKPPVSSQRSTSQRTAPAEPVRQKAARTRSPKSRVGGGILGKAIRAVLWVIIIVFVLYSAVSLIGILRMHRETAENRANISDVMDAAYVSNVLLIGTDSRDLSQERGRSDSMILASINKKTRELTLTSFLRDSYVYIDDEYGYGKLNAAYSYGGAGLLMDTIESNYGVRIDDYILISFAACANVIDAVGGVKLDLTDEEADAVNEILISEVNELMGDDRNDDLLDCGGKQKLDGKQALSYSRIRYVGNADFERASRQRTVMTQVLKKAAVNPVAMARIFVTALPELSTNLSVGKSYGYTLRAPILLIGYQLKTQQIPADDTFYGDDVDGESVLRVDCDANRQVLKDTIYRKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJN4</accession>
    <name>R5VJN4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">Phosphate acyltransferase</fullName>
            <ecNumber evidence="3 4">2.3.1.274</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Acyl-ACP phosphotransacylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Acyl-[acyl-carrier-protein]--phosphate acyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Phosphate-acyl-ACP acyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">plsX</name>
        <name evidence="5" type="ORF">BN566_01491</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the reversible formation of acyl-phosphate (acyl-PO(4)) from acyl-[acyl-carrier-protein] (acyl-ACP). This enzyme utilizes acyl-ACP as fatty acyl donor, but not acyl-CoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>a fatty acyl-[ACP] + phosphate = an acyl phosphate + holo-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:42292"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:14125"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:59918"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:138651"/>
            <dbReference type="EC" id="2.3.1.274"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism; phospholipid metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer. Probably interacts with PlsY.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="4">Associated with the membrane possibly through PlsY.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PlsX family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.274" evidence="3 4"/>
    <dbReference type="EMBL" id="CBAR010000161">
        <property type="protein sequence ID" value="CCZ83756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01491"/>
    <dbReference type="EnsemblBacteria" id="CCZ83756">
        <property type="protein sequence ID" value="CCZ83756"/>
        <property type="gene ID" value="BN566_01491"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00085"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043811">
        <property type="term" value="F:phosphate:acyl-[acyl carrier protein] acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00019">
        <property type="entry name" value="PlsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003664">
        <property type="entry name" value="FA_synthesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012281">
        <property type="entry name" value="Phospholipid_synth_PlsX-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30100">
        <property type="entry name" value="PTHR30100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02504">
        <property type="entry name" value="FA_synthesis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002465">
        <property type="entry name" value="Phsphlp_syn_PlsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00182">
        <property type="entry name" value="plsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="4" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="334" mass="35639" checksum="8310A968A79743B7" modified="2013-07-24" version="1">MNILIDAFGGDHAPLEVIQGAVRAVQELGVTVTLVGAENQIRQCAEEHHLSLDGLQILHAPAVFDIHEEPSTLLKQHADSSMAVGMQALHDGNGDAFVSAGSTGALLMGATFLVKRIRGVKRAALAPILPTATTPFMLLDAGANNDCRPEMLVQFAVMGSAYMERVMGIPQPKVKLLNVGAEETKGRELDLEAYRLLEQAPVHFCGNIEARELPFGVADVVVADGFTGNIALKLYEGMGKFMGSQLKENIFAGANGKLSALLVLPKIKALTKKMDYKAVGGAMMLGVQKPVVKAHGSSDATAFFNAIRQARDCVAGDMIAAMTKFMQQEQVKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJU3</accession>
    <name>R5VJU3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Arginine ABC transporter ATP-binding protein ArtM</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_01546</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000165">
        <property type="protein sequence ID" value="CCZ83811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01546"/>
    <dbReference type="EnsemblBacteria" id="CCZ83811">
        <property type="protein sequence ID" value="CCZ83811"/>
        <property type="gene ID" value="BN566_01546"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015424">
        <property type="term" value="F:ABC-type amino acid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030679">
        <property type="entry name" value="ABC_ATPase_HisP-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039085">
        <property type="entry name" value="ABC_ATPase_HisP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="240" mass="26844" checksum="790C3DC41F0226FA" modified="2013-07-24" version="1">MIRVENLHKYFGDLKVLKGINEHIQEGEVVAVIGPSGSGKSTFLRCLNLLETPTDGKIFVDDEQINAPKVDVNRIRQKMGMVFQQFNLFPHLTVMENLTLAPVLLKKMTKEQAVEKGKELLKRVGLAEKAESYPNQLSGGQKQRIAIARALEMNPEIMLFDEPTSALDPEMVGEVLDVMKDLAKSGMTMVVVTHEMGFAREVATRVLFMDQGIVMESGKPEDVFGNPQNERTKQFLSKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJV2</accession>
    <name>R5VJV2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Mg2+ and Co2+ transporters</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_01556</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000165">
        <property type="protein sequence ID" value="CCZ83821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01556"/>
    <dbReference type="EnsemblBacteria" id="CCZ83821">
        <property type="protein sequence ID" value="CCZ83821"/>
        <property type="gene ID" value="BN566_01556"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046873">
        <property type="term" value="F:metal ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045861">
        <property type="entry name" value="CorA_cytoplasmic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045863">
        <property type="entry name" value="CorA_TM1_TM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002523">
        <property type="entry name" value="MgTranspt_CorA/ZnTranspt_ZntB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01544">
        <property type="entry name" value="CorA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143865">
        <property type="entry name" value="SSF143865"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144083">
        <property type="entry name" value="SSF144083"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="251"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="286"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ83821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="309" mass="35488" checksum="6F664FB42B96DAEE" modified="2013-07-24" version="1">MISYYKTVQNHLVECSTPEPGSWVSVVDPTAQERKMLIEEFGLDSDFIKSALDEEESSRVEREDTQTLIIIDTAVSEIEKNDTLVFYTMPMSIIIKDQLVFTISLRNNKVIEDLIDGRLRNIQTSFRTQFVLRLIMQLTSTYLVYLKQIDKASNTLERELRKAVKNKQLLQMMELEKSLVYFSTSLKSNDATMNKILRGKLMKLYEDDQDLLEDVLIELKQAEEMASIYSSILANMVDACSSVISNNVNMVMWQLTIVTIILAIPTMVFSFYGMNTLDLPFAEHTWFPTVVSIVATLLTALILLKYKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>R5VK05</accession>
    <name>R5VK05_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">D-alanine--D-alanine ligase</fullName>
            <ecNumber evidence="7">6.3.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">D-Ala-D-Ala ligase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">D-alanylalanine synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ddl</name>
        <name evidence="11" type="ORF">BN566_01605</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Cell wall formation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + 2 D-alanine = ADP + D-alanyl-D-alanine + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:11224"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57416"/>
            <dbReference type="ChEBI" id="CHEBI:57822"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="8">Binds 2 magnesium or manganese ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the D-alanine--D-alanine ligase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.4" evidence="7"/>
    <dbReference type="EMBL" id="CBAR010000169">
        <property type="protein sequence ID" value="CCZ83876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01605"/>
    <dbReference type="EnsemblBacteria" id="CCZ83876">
        <property type="protein sequence ID" value="CCZ83876"/>
        <property type="gene ID" value="BN566_01605"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008716">
        <property type="term" value="F:D-alanine-D-alanine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00047">
        <property type="entry name" value="Dala_Dala_lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000291">
        <property type="entry name" value="D-Ala_lig_Van_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005905">
        <property type="entry name" value="D_ala_D_ala"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011095">
        <property type="entry name" value="Dala_Dala_lig_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011127">
        <property type="entry name" value="Dala_Dala_lig_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07478">
        <property type="entry name" value="Dala_Dala_lig_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01820">
        <property type="entry name" value="Dala_Dala_lig_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039102">
        <property type="entry name" value="Ddl/VanB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01205">
        <property type="entry name" value="D_ala_D_alaTIGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00843">
        <property type="entry name" value="DALA_DALA_LIGASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00844">
        <property type="entry name" value="DALA_DALA_LIGASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 7" id="KW-0133">Cell shape</keyword>
    <keyword evidence="6 7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 7 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="10">
        <location>
            <begin position="143"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR039102-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ83876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="357" mass="39378" checksum="A3E99EE10BF4E569" modified="2013-07-24" version="1">MAKTRVAVLFGGVSSEHEVSLMSATNVIRSIPKEKYEVICIGITKKGRWLYFPGDVSEIASGAWETNPDCTAAIISPDPIHKGIITIENGETYNKKIDVVFPVLHGRNGEDGRLQGLLELAKLPYVGCGTLSSALCMDKCMTHTVLDYNGIRTAKWACVSQREIGKMEETCQAIAETLGFPVFVKPANAGSSVGVNKATDMETLREAIQIAFSHDCKVVIEEFIDGKELEVAVFGYDAPFASYVGEIQPAAEFYDYEDKYITGTSQLFIPARITDAQSDMLRETAVKAYKALGCKGLSRVDFFLQKDGGIILNEINTLPGFTNISMYPKLMEHLGMSQEYLVDKLIEQAMENAERMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VK79</accession>
    <name>R5VK79_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Addiction module antitoxin RelB/DinJ family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01665</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000173">
        <property type="protein sequence ID" value="CCZ83936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83936">
        <property type="protein sequence ID" value="CCZ83936"/>
        <property type="gene ID" value="BN566_01665"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="72" mass="8304" checksum="D78709AC6BE33260" modified="2013-07-24" version="1">MSTKEQLMQELDTLTETQLKALLLFIHSFQTESSIPNEETAAALKEVSEMKQHPERYPGYTDVDTMMKDLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>R5VKM5</accession>
    <name>R5VKM5_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 13">Multifunctional fusion protein</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="13">Aspartate--tRNA ligase</fullName>
                <ecNumber evidence="13">6.1.1.12</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="13">Aspartyl-tRNA synthetase</fullName>
                <shortName evidence="13">AspRS</shortName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="12">Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B</fullName>
                <shortName evidence="12">Asp/Glu-ADT subunit B</shortName>
                <ecNumber evidence="12">6.3.5.-</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="12" type="primary">gatB</name>
        <name evidence="13" type="synonym">aspS</name>
        <name evidence="15" type="ORF">BN566_01777</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11 12">Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="function">
        <text evidence="13">Catalyzes the attachment of L-aspartate to tRNA(Asp) in a two-step reaction: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>ATP + H2O + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + 2 H(+) + L-asparaginyl-tRNA(Asn) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14513"/>
            <dbReference type="Rhea" id="RHEA-COMP:9674"/>
            <dbReference type="Rhea" id="RHEA-COMP:9677"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78515"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 12">
            <text>ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) + L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp)</text>
            <dbReference type="Rhea" id="RHEA:19649"/>
            <dbReference type="Rhea" id="RHEA-COMP:9660"/>
            <dbReference type="Rhea" id="RHEA-COMP:9678"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5 12">Heterotrimer of A, B and C subunits.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the GatB/GatE family. GatB subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the class-II aminoacyl-tRNA synthetase family. Type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.12" evidence="13"/>
    <dbReference type="EC" id="6.3.5.-" evidence="12"/>
    <dbReference type="EMBL" id="CBAR010000182">
        <property type="protein sequence ID" value="CCZ84051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01777"/>
    <dbReference type="EnsemblBacteria" id="CCZ84051">
        <property type="protein sequence ID" value="CCZ84051"/>
        <property type="gene ID" value="BN566_01777"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050566">
        <property type="term" value="F:asparaginyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004815">
        <property type="term" value="F:aspartate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006422">
        <property type="term" value="P:aspartyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02075">
        <property type="entry name" value="Asp_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00121">
        <property type="entry name" value="GatB"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
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    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
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    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
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    <dbReference type="InterPro" id="IPR004413">
        <property type="entry name" value="Apn/Gln-ADT_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017959">
        <property type="entry name" value="Asn/Gln-tRNA_amidoTrfase_suB/E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006075">
        <property type="entry name" value="Asn/Gln-tRNA_Trfase_suB/E_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018027">
        <property type="entry name" value="Asn/Gln_amidotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003789">
        <property type="entry name" value="Asn/Gln_tRNA_amidoTrase-B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004523">
        <property type="entry name" value="Asp-tRNA_synthase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042114">
        <property type="entry name" value="GatB_C_1"/>
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    <dbReference type="InterPro" id="IPR023168">
        <property type="entry name" value="GatB_Yqey_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017958">
        <property type="entry name" value="Gln-tRNA_amidoTrfase_suB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659">
        <property type="entry name" value="PTHR11659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02934">
        <property type="entry name" value="GatB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02637">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00845">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="SSF55931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89095">
        <property type="entry name" value="SSF89095"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00458">
        <property type="entry name" value="aspS_nondisc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00133">
        <property type="entry name" value="gatB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01234">
        <property type="entry name" value="GATB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II" evidence="14">
        <location>
            <begin position="659"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="region of interest" description="Aspartate" evidence="13">
        <location>
            <begin position="714"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="692"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="735"/>
            <end position="737"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="735"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="879"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="882"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="886"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="927"/>
            <end position="930"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CCZ84051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="956" mass="107609" checksum="FDB039B4FDA83D43" modified="2013-07-24" version="1">MSSYIPVIGLEIHAELLTKSKVFCTCSAEFGGSPNSRCCPVCTGMPGTLPVINQTAVQYAVKAGFALGCNINKFSVFDRKNYFYPDLPKAYQISQLQRPLCIDGLVEIETEKGKKNIRINRIHLEEDAGKLIHDDFNAVSLADYNRCGIPLIEIVTEPDIGSAEEAKAFVEKISLLLQYAGVCDCKMEQGSLRCDVNISIMKPEDKELGTRAEIKNLNSLKAITRAINYEIKRQSRLLDAGKRVIQETRRFNDNKGETTSMRSKENAHDYRYFPEPDILQVNFTDEMLDNIKNMLPELPHKRLQRYIHSYGLSEADAKIIVNQKRVSDFFEDAMKTYAAPKSLANFIIGELMRRINLGEVTMETLPFSASEFAKLVEMADTEKVSKNDAKKILRAMVETGEDAESIAKKQGMLIVNDLAKAKEQIAAILSANETAVVQYQNGEQKVFGFLMGQCTKALRGICTPKVIKETLETALKQPVAAKETVAEETAAKTETAPEEVAYTEYHNDAAYRPTVKDKILQVDGNTLLPEFDYEAARNQIGEEISLKACVHKIRQMSSFAFIILRTGRYLMQAIYDPSVCAEKMDGIREGNFVSVTGVVKENEKAVGGLELQLKHLTVISASNEEVPLKIFNPKLGCSLDINLDNRAVSLRNVNERAIFKIQEGVANGFRQFMLSQGFTEIHTPKIVAQGAEGGANIFHLEYFDKPAFLNQSPQFYKQTAVAFFDRVFEIAPVYRAEKHATSRHINEYIGLDFEMGYIDSMYDVMAMETATIRYIFSYLQEHYAHELEMLHADVPVVKEIPCVTLLEAKEILGNKGSKNKLDLEPEDEVALCEYAKKTFDSDFIFVTHFPSSKPPFYAMNSKEDPRLAYKFDLLFRGLEITSGGQRIHNYQEQVDKIIAQGLNPDDFHYYLEAHKYGLPPHGGLGIGLERFVMKLLGLSNVRQASMFPRDINRLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5VKX8</accession>
    <name>R5VKX8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative efflux protein MATE family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01911</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84186">
        <property type="protein sequence ID" value="CCZ84186"/>
        <property type="gene ID" value="BN566_01911"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006603">
        <property type="entry name" value="DinF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="210"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="252"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="297"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="331"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="374"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="402"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="432"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="464" mass="50919" checksum="6AA2B32A528628E7" modified="2013-07-24" version="1">MTTNTKAKPRKADKMRSTPPHQLLLKMGIPMICSMVLQAMYNIVDSAFVSNMPENGEAALNALTLAFPMQMLMIAVSIGTGVGVNALVSKSLGQGDTEKASRTAGNGGFLAVLIFLLCVFFGIFGVAPYIHLQTKDFLIADMGISYLRICCILSVGIIFFSIYEKLLQASGNPIFSTIAQISGAVCNILLDPILIYGWCGLPELGVRGAAYATVLGQILSFLLAWIFHCKVDKQISNKLQYWKPNSTIIREIYAIGLPAILAQALMSVMTYGMNLILGSVNTALVTAYGLYYKIQQFILFAAFGLRDAITPVLSFYHGMRSRARIRYGIRYGMLYTWIIMLAGTLLIEVLAIPFAKLFGLSGETEQLCIEAMRIISISFLFAGTNIALQGIFQALDSGLESLIISVCRQILFVLPVAWLFSLTAKANFNQSWLIWLTFPIAELVSMGIGFGCLHRIQKTKIAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>R5VL03</accession>
    <name>R5VL03_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Holliday junction ATP-dependent DNA helicase RuvA</fullName>
            <ecNumber evidence="8">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ruvA</name>
        <name evidence="10" type="ORF">BN566_01941</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvA stimulates, in the presence of DNA, the weak ATPase activity of RuvB.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Forms a complex with RuvB.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the RuvA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="8"/>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01941"/>
    <dbReference type="EnsemblBacteria" id="CCZ84216">
        <property type="protein sequence ID" value="CCZ84216"/>
        <property type="gene ID" value="BN566_01941"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009379">
        <property type="term" value="C:Holliday junction helicase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14332">
        <property type="entry name" value="UBA_RuvA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00031">
        <property type="entry name" value="DNA_helic_RuvA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013849">
        <property type="entry name" value="DNA_helicase_Holl-junc_RuvA_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003583">
        <property type="entry name" value="Hlx-hairpin-Hlx_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000085">
        <property type="entry name" value="RuvA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011114">
        <property type="entry name" value="RuvA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036267">
        <property type="entry name" value="RuvA_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07499">
        <property type="entry name" value="RuvA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01330">
        <property type="entry name" value="RuvA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00278">
        <property type="entry name" value="HhH1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46929">
        <property type="entry name" value="SSF46929"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="SSF47781"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00084">
        <property type="entry name" value="ruvA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6 8" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 8 10" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0742">SOS response</keyword>
    <feature type="domain" description="HhH1" evidence="9">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="HhH1" evidence="9">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ84216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="204" mass="21602" checksum="A1871B0E796D0CA0" modified="2013-07-24" version="1">MIDSLHGTLIAKEMNMAVVECGGVGYACRISYQTYSKLCAVGEEVFLYTILSVSMREGNLELYGFLSAQERRCFSMLTGVGSVGGKAALAILSELTPERLALVVASGDSKTLTKVKGIGTKMAQRIVLELKDKIAKEQGGVLSAANLSAEVAPPAESEVAEALSALTVLGYTQLEVMPILAKLPAELSATELIKETLKAIGKRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5VLF7</accession>
    <name>R5VLF7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">HAD-superfamily phosphatase subfamily IIIC/FkbH-like domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00288</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000222">
        <property type="protein sequence ID" value="CCZ84381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00288"/>
    <dbReference type="EnsemblBacteria" id="CCZ84381">
        <property type="protein sequence ID" value="CCZ84381"/>
        <property type="gene ID" value="BN566_00288"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010037">
        <property type="entry name" value="FkbH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010033">
        <property type="entry name" value="HAD_SF_ppase_IIIC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01686">
        <property type="entry name" value="FkbH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01681">
        <property type="entry name" value="HAD-SF-IIIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="599" mass="68729" checksum="E3EDC1DCB75C849E" modified="2013-07-24" version="1">MKELQVPFDSAWILKNRRKIKKALLADGTPRMEKKIAVLGGSTTNDIVSMLELFLLDNGIQPSFYQSEYARYWQDAMFENPELEAFQPDLIFIHTTSRNITEFPEDVYCPAETVEAALERQYTHFTQMWDRLAEGYHCPIIQNNFELPAYRLLGNRDASDIHGRINFITRLNCKFYDYANTHTSFYIHDIQYLSACYGLDAWAEPSYWYLYKYALSVPAIPDFAYNLTRIIRSIYGKNKKVLALDLDNTLWGGVIGDDGQEGIEIGHETSMGQAYEELQGYLKQQKKIGVLLTVCSKNEPENALLGLNHPSGVLKPDDFVTIKANWEPKDRNLVETAAELNLLPESIVFVDDNPAERAIVSGQIPGVAVPEFDCVEDCLRQLDRAGYFEVTSLSSDDAKRNAMYQANAQRAQMQKTFADYTDYLNSLEMQGTIRDFEPIYLQRIVQLTNKSNQFNLTTKRYTQSEMETVAASDRYIRLYGRLEDKFGDNGIVSVVIGEKVDDALHMDLWLMSCRVLKRDMEFAMLDELVRQCREQGIQNIVGYYYKTAKNAMVKDLYGTFGFTKTKDMENGDSVWELNVADYTNKNHVITVNQKECAEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>R5VLG9</accession>
    <name>R5VLG9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Proline--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Prolyl-tRNA synthetase</fullName>
            <shortName evidence="8">ProRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">proS</name>
        <name evidence="10" type="ORF">BN566_00298</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro)</text>
            <dbReference type="Rhea" id="RHEA:14305"/>
            <dbReference type="Rhea" id="RHEA-COMP:9700"/>
            <dbReference type="Rhea" id="RHEA-COMP:9702"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78532"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.15"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.15" evidence="8"/>
    <dbReference type="EMBL" id="CBAR010000223">
        <property type="protein sequence ID" value="CCZ84391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00298"/>
    <dbReference type="EnsemblBacteria" id="CCZ84391">
        <property type="protein sequence ID" value="CCZ84391"/>
        <property type="gene ID" value="BN566_00298"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004827">
        <property type="term" value="F:proline-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006433">
        <property type="term" value="P:prolyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00778">
        <property type="entry name" value="ProRS_core_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01571">
        <property type="entry name" value="Pro_tRNA_synth_type3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002316">
        <property type="entry name" value="Pro-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004499">
        <property type="entry name" value="Pro-tRNA-ligase_IIa_arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016061">
        <property type="entry name" value="Pro-tRNA_ligase_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017449">
        <property type="entry name" value="Pro-tRNA_synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033721">
        <property type="entry name" value="ProRS_core_arch_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43382">
        <property type="entry name" value="PTHR43382"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09180">
        <property type="entry name" value="ProRS-C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01046">
        <property type="entry name" value="TRNASYNTHPRO"/>
    </dbReference>
    <dbReference type="SMART" id="SM00946">
        <property type="entry name" value="ProRS-C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64586">
        <property type="entry name" value="SSF64586"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00408">
        <property type="entry name" value="proS_fam_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II" evidence="9">
        <location>
            <begin position="45"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ84391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="481" mass="54426" checksum="854F70DC5DC0FA3C" modified="2013-07-24" version="1">MAKNNQKMVDAITSMDEDFAQWYTDIVKKAELSSYTSVKGCMVIRPYGYAIWEQMQHILDETFKETGHENVCMPMFIPESLLQKEKDHVEGFAPEVAWVTHGGSEKLEERLCVRPTSETLFCDHYAEIVHSYRDLPKLYNQWVSVVRWEKTTRPFLRSREFLWQEGHTIHATAEEALAETEQMLNVYADFCEKSLAMPVVKGRKTESDKFAGAVATYAIEALMHDGKALQAGTSHYFGDGFAKAFNIQFTDKNNTLQYPHQTSWGVTTRLIGAIIMTHGDNNGLVLPPAVAPIQVIIVPVAMHKEGVLDKAKALEAQLKAAGIRVKVDDTDNSPGWKFAQYEMKGVPVRLEIGPKDIEQDQCVLVTRHNREKTFCPLSQLVETVQERLQAVHDGMYEKALENRKNRTYVCKNMEEIQQVLAEKGDGFVEAMWCGDEACEDKVKEVTGVGSRCIPLEQKHHADTCVCCGKPAKHMVLWAKAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5VLL0</accession>
    <name>R5VLL0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_00338</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the autoinducer-2 exporter (AI-2E) (TC 2.A.86) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000225">
        <property type="protein sequence ID" value="CCZ84431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00338"/>
    <dbReference type="EnsemblBacteria" id="CCZ84431">
        <property type="protein sequence ID" value="CCZ84431"/>
        <property type="gene ID" value="BN566_00338"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002549">
        <property type="entry name" value="AI-2E-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21716">
        <property type="entry name" value="PTHR21716"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01594">
        <property type="entry name" value="AI-2E_transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="81"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="178"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="248"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="275"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="307"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="338"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="420"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="420"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="471"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ84431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="489" mass="54535" checksum="0F4F8311AA249F3F" modified="2013-07-24" version="1">MPKRKRNSINTPFARTIIFSGVILLLVAFLLMRLETVSTWIGTILSTLRPVIIGAVITLVLHTPVRRLEGFLKNITKGKRFPCSAVAVILSYLLLFSVLAGIVCIIVPQFSQSLVDFADQFTIYFINFQKFLQTYSTKGQQLYDLMQQVGLDFAQLKSWLTDLSVTVSSYVPNLMEKIGTWATSLVRIVADCFIGLIFSLYMLSGRKRLKAQAKRILQKAFSKENYHRISHYGNITFEIFSNFVGGQLMDACILGLLCFIGMSIFHFQYPLLISVIIGLTNMIPIVGPIVGTIPGALILLLVEPKQAIWFVVFVIVIQQIDSNLIYPRVVGGSVGLPAMWVLFAVIVGGGLFGVLGMILGVPMMSLIYVILREKTLPEGEVPPSPLQMPERTAMEQRANEWFGKMRDTVFTTVTDTVKRNWQHHEEDDDSATEEPTEKDMKSAESTVSETPISVILEQTDEEGCEIHPAASESESTDSDSPEQPPEQNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>R5VLQ3</accession>
    <name>R5VLQ3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Uracil phosphoribosyltransferase</fullName>
            <ecNumber evidence="3 11">2.4.2.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">UMP pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">UPRTase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">upp</name>
        <name evidence="12" type="ORF">BN566_00370</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the conversion of uracil and 5-phospho-alpha-D-ribose 1-diphosphate (PRPP) to UMP and diphosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>diphosphate + UMP = 5-phospho-alpha-D-ribose 1-diphosphate + uracil</text>
            <dbReference type="Rhea" id="RHEA:13017"/>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Binds 1 Mg(2+) ion per subunit. The magnesium is bound as Mg-PRPP.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="11">Allosterically activated by GTP.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uracil: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the UPRTase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.9" evidence="3 11"/>
    <dbReference type="EMBL" id="CBAR010000238">
        <property type="protein sequence ID" value="CCZ84471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00370"/>
    <dbReference type="EnsemblBacteria" id="CCZ84471">
        <property type="protein sequence ID" value="CCZ84471"/>
        <property type="gene ID" value="BN566_00370"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00574">
        <property type="reaction ID" value="UER00636"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004845">
        <property type="term" value="F:uracil phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044206">
        <property type="term" value="P:UMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006223">
        <property type="term" value="P:uracil salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01218_B">
        <property type="entry name" value="Upp_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034332">
        <property type="entry name" value="Upp_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005765">
        <property type="entry name" value="Ura_phspho_trans"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01091">
        <property type="entry name" value="upp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="5 11 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11 12" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="5-phospho-alpha-D-ribose 1-diphosphate binding" evidence="11">
        <location>
            <begin position="130"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Uracil binding" evidence="11">
        <location>
            <begin position="198"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>uracil</name>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CCZ84471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="208" mass="22551" checksum="AA47A561DCFCDE77" modified="2013-07-24" version="1">MALHIVTHPLVQHKLSLMRNKGTGVKEFRELTEETAMLICYEAMRDLPLKTVKLETPQGWSETKIISGKKLAFVPILRAGLGLIEGVTSLIPAAKIGHIGIFCEPGTHDAVKYYSKMPDDIQEREVVVIDAMLATGSSAIAAIQDLKDMGVKNIKFLCILCSPEGKNALEAAHPDVDIFCAGMDEGLNEDGYIVPGLGDAGDRMFGTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VM80</accession>
    <name>R5VM80_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00504</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000266">
        <property type="protein sequence ID" value="CCZ84651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84651">
        <property type="protein sequence ID" value="CCZ84651"/>
        <property type="gene ID" value="BN566_00504"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="91" mass="10611" checksum="8BCE81DD254B0A55" modified="2013-07-24" version="1">MMQVEVKSGTMTKDEIQYYILLVKKRWGTRLPADLRLILEKTDAEVQITFDPPIDRQVYRSTDYLVNDVQKLNRGKFAEHADKVMHVTATV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5VQD1</accession>
    <name>R5VQD1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00597</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000008">
        <property type="protein sequence ID" value="CCZ82792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82792">
        <property type="protein sequence ID" value="CCZ82792"/>
        <property type="gene ID" value="BN566_00597"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004395593" evidence="2">
        <location>
            <begin position="24"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="181"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ82792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="212" mass="22380" checksum="6A761DDA4DD1E91A" modified="2013-07-24" version="1">MKIPMIGFAFTMTACFLMTGCSGVGVGGGSTTSSNQLNTAFTTGVTMELEEMTAEGTMQRVGAGEWNVSFSEPSQLAGVRLDFQKGEVTASYKGLAFSVPQTALPAKSILYQFILAADQLAEESTLQGTSKDGGMVLSGDLEAGSYELTMTAEGVPAIFSMKNQNAVLTFHDFVSNGAVSESETSIESELQTTEPALQTESTELETTLESQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>R5VQN7</accession>
    <name>R5VQN7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ATPases with chaperone activity ATP-binding subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN566_00689</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the ClpA/ClpB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000022">
        <property type="protein sequence ID" value="CCZ82887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00689"/>
    <dbReference type="EnsemblBacteria" id="CCZ82887">
        <property type="protein sequence ID" value="CCZ82887"/>
        <property type="gene ID" value="BN566_00689"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1780.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036628">
        <property type="entry name" value="Clp_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004176">
        <property type="entry name" value="Clp_R_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001270">
        <property type="entry name" value="ClpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018368">
        <property type="entry name" value="ClpA/B_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028299">
        <property type="entry name" value="ClpA/B_CS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041546">
        <property type="entry name" value="ClpA/ClpB_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17871">
        <property type="entry name" value="AAA_lid_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02861">
        <property type="entry name" value="Clp_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00300">
        <property type="entry name" value="CLPPROTEASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81923">
        <property type="entry name" value="SSF81923"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51903">
        <property type="entry name" value="CLP_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00870">
        <property type="entry name" value="CLPAB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00871">
        <property type="entry name" value="CLPAB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Clp R" evidence="8">
        <location>
            <begin position="4"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="658"/>
            <end position="685"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ82887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="750" mass="83154" checksum="BF9D494B2643B7F1" modified="2013-07-24" version="1">MRYTDGFTGKAILALKEAICAAEELGHTYIGSEHLLLGLLEEGSNAAAAMLAAQHITAQRVRNALVELVGRGIPAQVQEDCLTPALSEIMQQAKAFSKEDGRSLAGTEHLLRSILRTPCCTAVAILKKLGVSLNQLGAVSRQETSCHLRMEERRTERISKKTLPNLFQYGKWMTDPALLHQYDPVIGREAELQQMVQILTRRTKNNPCLVGDAGVGKTAIVEGVAQKILRGEVPEALLPQHIFSLDLPALLSGAKYRGDFEERFRNCLQEATQNEQIILFIDELHTIVGAGAAEGAIDAANMLKPQLARGEVKLIGATTTEEFRKYIEKDSALERRFQPIQVLEPSPEACFSILCGLRETYANFHHIEIPDAILRQAVSCSVRYLHDRYLPDKAIDLLDEACSRARIRCEQEHVSCPVVTESDLAEIVSMRIGIPVQKITTAQQQRLMTLEQELQQQIIGHTAAIRQLSAALIRARTGLREENRPIGCFLFTGPTGVGKTALAKAAALHLFDDKDSLIRFDMSEYMEKHTVSKLIGAPPGYVGYEEGGTLCERVRKRPYCVILFDEIEKAHPDICNILLQIMEDGVLSDSNGRKTDFTNALLILTSNLGGTGSKPPAGFVQTTQATSETALRQYFSPEFLGRLDAIIPFQPLTQEELCQIAEKQLQNLQKRMEQLHVQLHFTQEAVQQLAHAPDTGRYGARPMRRYLTEAVETQLAQQLLQKTIAAGDQVLLSANEHTFSLTKETTTVHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5VR61</accession>
    <name>R5VR61_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA replication protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00815</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000039">
        <property type="protein sequence ID" value="CCZ83022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00815"/>
    <dbReference type="EnsemblBacteria" id="CCZ83022">
        <property type="protein sequence ID" value="CCZ83022"/>
        <property type="gene ID" value="BN566_00815"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002611">
        <property type="entry name" value="IstB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01695">
        <property type="entry name" value="IstB_IS21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="180"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="336" mass="38674" checksum="E3C59DEF8C3078D0" modified="2013-07-24" version="1">MNERYLQQALSAISVRRNNAQTIQQQHLAEIAQTVPEIMEINQQLFHTSQDLIQIIRSGENVAERTQRLAQQNQQGQAMIRKLLVQHGYPQNYLDIVYQCPDCNDTGYANGTFCHCLLKLAGKLATKELNKSAQVQLCRFDTFSLDYYRGKTTEHGSDCYTAMQKILAYCQNYAQYFHKSSPSILMFGKTGLGKTHLSLSIASVALERGYSVLYDSVINFLRKIEREHFGKEHPETDTMELLLTCDLLILDDLGTEFDSSFYQSTIYNLINTRLNRSLPTIISTNLDFNGISNRYEERITSRIYANYSILRFVGQDVRIMKAQQAFRKEKERGNRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5VR88</accession>
    <name>R5VR88_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00850</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000047">
        <property type="protein sequence ID" value="CCZ83062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00850"/>
    <dbReference type="EnsemblBacteria" id="CCZ83062">
        <property type="protein sequence ID" value="CCZ83062"/>
        <property type="gene ID" value="BN566_00850"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029432">
        <property type="entry name" value="Sipho_Gp37"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14594">
        <property type="entry name" value="Sipho_Gp37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="373" mass="42549" checksum="D354B7E98E9E4A33" modified="2013-07-24" version="1">MLIEVYQMTAAENTVSITLEAVCDAFSSFLWDIEYFQCGQFELYIAATPETVAVFQTGRLVGRKDDTEHYGLIESVRIQTDAENGDYLTVSGHFLMILLSRRIIYPRMVIKEQTSYGEIIHTAIHKNCLQQNERFLPGLQLGEITGDCWKQETHLQISYANLMEWIYKICELVGGTANISLVETKPNSRTYQMVFTLSEGVDRSILQDTYPHVIFSDAFHNLLTFDYLKNAAAQQNAAYTLGAGEGEARKRAFCTIDPEPTQWERYEVYVDARDLSEETQNDAGESITIPEEEYLKMLEERGRENFLPVEEISESSITATSTQEQYPQDYQVGDLVTVQQTRFGLSQNRIRLIGMIESFDQNGRSLTPTFQEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5VRY0</accession>
    <name>R5VRY0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glucose-1-phosphate adenylyltransferase GlgD subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01098</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial/plant glucose-1-phosphate adenylyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01098"/>
    <dbReference type="EnsemblBacteria" id="CCZ83317">
        <property type="protein sequence ID" value="CCZ83317"/>
        <property type="gene ID" value="BN566_01098"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008878">
        <property type="term" value="F:glucose-1-phosphate adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011831">
        <property type="entry name" value="ADP-Glc_PPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011832">
        <property type="entry name" value="GlgDAde_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43523">
        <property type="entry name" value="PTHR43523"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43523:SF6">
        <property type="entry name" value="PTHR43523:SF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02092">
        <property type="entry name" value="glgD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NTP_transferase" evidence="2">
        <location>
            <begin position="18"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="374" mass="40945" checksum="4AC3EDA118B4223E" modified="2013-07-24" version="1">MGMNNKVLGLIFASINDTNVIDLTKLRTMGSIPFGGRYRLIDFPLSNMVNSGISEVGVITKSNYGSLLDHLGSGREWDLTGKTGGLHLLPPFSHVGGGIYQGRLEALNNVREFVEHSKSEYVVLSNCDVVTNIDFNAALQQHIDTEADITIIYSKGFYSKEKNPSTNVISVDENHTVKEVLITPAITGECNVSLEMFIMRTEFLKKIVQEAASKSQHSLVRDVLQGRKDEFVIKGYEHKGVFTKIDGLAAYYQANLSLLQADTRAGLFQESAPIYTKVGDNAPVKYGLESSVKNSLIADGCVIEGSVENCVLFRGVRIGKDAVVKNSVLMQGTHVGEKCNIERVIADKNVDISSDKVLTGSDDFPLYLSKNGKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5VSF7</accession>
    <name>R5VSF7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transcriptional regulator BadM/Rrf2 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01236</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000110">
        <property type="protein sequence ID" value="CCZ83462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01236"/>
    <dbReference type="EnsemblBacteria" id="CCZ83462">
        <property type="protein sequence ID" value="CCZ83462"/>
        <property type="gene ID" value="BN566_01236"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000944">
        <property type="entry name" value="Tscrpt_reg_Rrf2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33221">
        <property type="entry name" value="PTHR33221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02082">
        <property type="entry name" value="Rrf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00738">
        <property type="entry name" value="rrf2_super"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51197">
        <property type="entry name" value="HTH_RRF2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="144" mass="16150" checksum="FB557D483233C0B1" modified="2013-07-24" version="1">MKISTKGRYALRMMLDLAIHQNEGFVSLKDIADRQGISKKYLEQIVPLLNRTGMLRTNRGYQGGYRLAKSPDAYTVGEILRLTEGDLAPVACLQGDTVNCERADHCITLPVWKGLYHVINDYLDHITLQDVLNQAPEQGGVFCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>R5VSG2</accession>
    <name>R5VSG2_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glycine--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Glycyl-tRNA synthetase</fullName>
            <shortName evidence="8">GlyRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">glyQS</name>
        <name evidence="10" type="ORF">BN566_01241</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the attachment of glycine to tRNA(Gly).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly)</text>
            <dbReference type="Rhea" id="RHEA:16013"/>
            <dbReference type="Rhea" id="RHEA-COMP:9664"/>
            <dbReference type="Rhea" id="RHEA-COMP:9683"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78522"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.14"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.14" evidence="8"/>
    <dbReference type="EMBL" id="CBAR010000111">
        <property type="protein sequence ID" value="CCZ83467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01241"/>
    <dbReference type="EnsemblBacteria" id="CCZ83467">
        <property type="protein sequence ID" value="CCZ83467"/>
        <property type="gene ID" value="BN566_01241"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004820">
        <property type="term" value="F:glycine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006426">
        <property type="term" value="P:glycyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00774">
        <property type="entry name" value="GlyRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00253_B">
        <property type="entry name" value="Gly_tRNA_synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027031">
        <property type="entry name" value="Gly-tRNA_synthase/POLG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022961">
        <property type="entry name" value="Gly_tRNA_ligase_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033731">
        <property type="entry name" value="GlyRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002315">
        <property type="entry name" value="tRNA-synt_gly"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10745">
        <property type="entry name" value="PTHR10745"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01043">
        <property type="entry name" value="TRNASYNTHGLY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00389">
        <property type="entry name" value="glyS_dimeric"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II" evidence="9">
        <location>
            <begin position="151"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="218"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="327"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="203"/>
            <end position="205"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="213"/>
            <end position="218"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="287"/>
            <end position="288"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="331"/>
            <end position="334"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ83467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="462" mass="53575" checksum="522516CEEF6A0ECD" modified="2013-07-24" version="1">MLTNQEKSMDKIVDLCKSRGFVYAGSEIYGGLANTWDYGPLGVELKNNIKRAWWQKFVQECPYNVGLDSAILMNPQTWVASGHLGGFSDPLMDCKACKTRHRADNLIEDFDGTNPAGWSNEEMMNYIREKQIPCPNCGKSDFTDIRQFNLMFKTFQGVTEDSKSELYLRPETAQGIFVDFANIQRTTRKKVPFGVGQIGKSFRNEITPGNFIFRVREFEQMELEFFCKPDTDLEWFQYWRGYCKDFLLNLGIKEENLRLRDHDAEELCFYSKATTDFEYRFAFGWGELWGVADRTNYDLTQHMNHSGKSMEYFDPEENRKYIPYVIEPSLGVERLFLSIVTEAYDEEVVDAEKNDTRVVMHFHPALAPYKAAILPLSKKLSGKAQEVYAQLSKKFMIDFDDTGSIGKRYRRQDEIGTPFCITYDFDTLEKDNCVTVRNRDTMEQVRIPIADLEAYLSEKVAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5VT01</accession>
    <name>R5VT01_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01403</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000148">
        <property type="protein sequence ID" value="CCZ83667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83667">
        <property type="protein sequence ID" value="CCZ83667"/>
        <property type="gene ID" value="BN566_01403"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="5"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="165" mass="19619" checksum="007F8BE0EFA6AB9A" modified="2013-07-24" version="1">MNDIEKKLEALKAEFLGKLEALRKEAEAQKKQEEPKPWKPEAGEEYFFVNNDLSIYCFCNYNGEEDRYNFEIGNCFRTGERAEQVAEKMRLLLRLEQLHDMICPDYVPDWNNDGELKFCLAHHCDDDCWFVDYFNVTQYPTVYFDTEENAQKAAEILNKEMRESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5VT11</accession>
    <name>R5VT11_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01413</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000148">
        <property type="protein sequence ID" value="CCZ83677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01413"/>
    <dbReference type="EnsemblBacteria" id="CCZ83677">
        <property type="protein sequence ID" value="CCZ83677"/>
        <property type="gene ID" value="BN566_01413"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="109" mass="13104" checksum="42A4C73C9C04AD66" modified="2013-07-24" version="1">MTYTDIAKMMEQMHLPFAYHHFERGKAPPLPYFVFYYDGRSDFSADNHAYQKIVEVTLELYSNQKDFKSESQIESVLERNEIVYDKTEEYISSEKMFEQIYEFELLLEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5VT17</accession>
    <name>R5VT17_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF2344 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01418</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000151">
        <property type="protein sequence ID" value="CCZ83682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01418"/>
    <dbReference type="EnsemblBacteria" id="CCZ83682">
        <property type="protein sequence ID" value="CCZ83682"/>
        <property type="gene ID" value="BN566_01418"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018768">
        <property type="entry name" value="DUF2344"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10105">
        <property type="entry name" value="DUF2344"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03936">
        <property type="entry name" value="sam_1_link_chp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2344" evidence="1">
        <location>
            <begin position="7"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="221" mass="25139" checksum="5C7921A52437635A" modified="2013-07-24" version="1">MSEKFEKLRFRVAKTGRAKYISHLDFVRCVQRAIRRSGLPVHYTNGFHPHMEAVFATALPLGMESIGEVLEIRFAEPVEPEAARTCLQASLPEGMKLLKVYQPVHKMGELAYVRYRVTIPSEQPERLFEQWHAFLQQPEVLVEKKSKRGMRTVDLLPQIDCQAEQVIVGALVLEWMLSVGQPESVNPNLIVTAFTLFAGVSAEQVQICRTQLCTAERTEFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5VTD2</accession>
    <name>R5VTD2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Fibronectin type III domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01532</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000164">
        <property type="protein sequence ID" value="CCZ83797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01532"/>
    <dbReference type="EnsemblBacteria" id="CCZ83797">
        <property type="protein sequence ID" value="CCZ83797"/>
        <property type="gene ID" value="BN566_01532"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002105">
        <property type="entry name" value="Dockerin_1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00404">
        <property type="entry name" value="Dockerin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14200">
        <property type="entry name" value="RicinB_lectin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="SSF50370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ricin B-type lectin" evidence="1">
        <location>
            <begin position="34"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="2">
        <location>
            <begin position="138"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="200" mass="22570" checksum="33247B5C78B3A2FE" modified="2013-07-24" version="1">MSVNMNLKVQKYLTAESNNNVDVKSKISRFGSPEQVWKFTRKEDGSYKITNCKNKLALDSTETTEPKANVYTYQDCNNECQSWDLFEQSDSYFLKSKYTECVMDIDGGKTDEGTNVQMYTKNDTAAQAFQILKIENYTIKIPGDLNQDGTLSLTDLIPLQQSLLRQTTLTADQAQLADYNGDGSINVLDLMLLKRALLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VU84</accession>
    <name>R5VU84_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01798</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000186">
        <property type="protein sequence ID" value="CCZ84072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84072">
        <property type="protein sequence ID" value="CCZ84072"/>
        <property type="gene ID" value="BN566_01798"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="41" mass="4895" checksum="2131ED5CB0415D2A" modified="2013-07-24" version="1">MIFGFLQAIMDSAVIQLVRDWIFQLLSKIQQEYNDYNLITD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>R5VUG3</accession>
    <name>R5VUG3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ZT_dimer domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_01887</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000193">
        <property type="protein sequence ID" value="CCZ84162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01887"/>
    <dbReference type="EnsemblBacteria" id="CCZ84162">
        <property type="protein sequence ID" value="CCZ84162"/>
        <property type="gene ID" value="BN566_01887"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027470">
        <property type="entry name" value="Cation_efflux_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036837">
        <property type="entry name" value="Cation_efflux_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16916">
        <property type="entry name" value="ZT_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160240">
        <property type="entry name" value="SSF160240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="SSF161111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="25"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="ZT_dimer" evidence="7">
        <location>
            <begin position="230"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF16916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ84162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="401" mass="45028" checksum="C81E82089A73C145" modified="2013-07-24" version="1">MLTNFFVNRMIPDPKNIENPSVRKAYGTLSSVVCMVCNILLFSIKYLAGSLSGSIAILSDAFNNLSDCANCLITFFGYRMAAKPADKGHPFGHGRMEYLTSLLIAVLIAIMGVELLRDSFDKLRHPAPVTFRWLVLGILLLSILIKLWMALFNQKLGKQCRSSAILATAKDSYSDVLATTSTLIVLVVSRFTDFPLDGWMGILVSLFILKTAYDIIKDTVDDLLGKPADAALVKQICQLIRSHPEILGIHDLVIHNYGMGKRIGSCHVEVSSHSDFVAIHDLVDNIEHQIQNQLQINMTIHMDPMELDNEQINACRSMLLHILHQLDPHLHMHDFRMVAGETHTNLIFDLVVPFDCVYKNDQLKEMIESALAAQPIQYYTIITFDREYTAIDSETDSGENF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5VUH7</accession>
    <name>R5VUH7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01907</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01907"/>
    <dbReference type="EnsemblBacteria" id="CCZ84182">
        <property type="protein sequence ID" value="CCZ84182"/>
        <property type="gene ID" value="BN566_01907"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="158"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="216"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="242" mass="27314" checksum="43F77B95D20F64BA" modified="2013-07-24" version="1">MFSLYKKELQSYFLSPAAYAVLAFFTLTFSLTMIMDISAASSLYEFSFTSLFYTNLFYLAILFPVLTMRSFSEERKNGTEVLLLSSPISVTKIVLAKFLATATIFLIMLAVSLFYPIVTALNGHVILANLICTYVGFYLFGLVCIALGILISSFTESIILSIIMSEAAIIILLLMDNIGTYSFFSNLPILSDLLHWLSNQQKFITFSNGYMQVSDLFAYCTEIAVFLILTVISVEKRRWSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVK6</accession>
    <name>R5VVK6_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8">30S ribosomal protein S3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rpsC</name>
        <name evidence="13" type="ORF">BN566_00063</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 8">Binds the lower part of the 30S subunit head. Binds mRNA in the 70S ribosome, positioning it for translation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Part of the 30S ribosomal subunit. Forms a tight complex with proteins S10 and S14.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 10">Belongs to the universal ribosomal protein uS3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000241">
        <property type="protein sequence ID" value="CCZ84517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00063"/>
    <dbReference type="EnsemblBacteria" id="CCZ84517">
        <property type="protein sequence ID" value="CCZ84517"/>
        <property type="gene ID" value="BN566_00063"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.32">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01309_B">
        <property type="entry name" value="Ribosomal_S3_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005704">
        <property type="entry name" value="Ribosomal_S3_bac-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001351">
        <property type="entry name" value="Ribosomal_S3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036419">
        <property type="entry name" value="Ribosomal_S3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018280">
        <property type="entry name" value="Ribosomal_S3_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00189">
        <property type="entry name" value="Ribosomal_S3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="SSF54814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54821">
        <property type="entry name" value="SSF54821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01009">
        <property type="entry name" value="rpsC_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50823">
        <property type="entry name" value="KH_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00548">
        <property type="entry name" value="RIBOSOMAL_S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 8 10" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 8 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 8" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="KH type-2" evidence="12">
        <location>
            <begin position="39"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="213"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CCZ84517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="265" mass="30079" checksum="F0DC1C931537487E" modified="2013-07-24" version="1">MGQKVNPHGLRVGVIKDWDSRWFANKADFGDTLVEDFNVRKFIKKNLYAAGVPKIEIERFADKVRIHIHCAKPGIVIGRGGAEIEKLRTQLEKMMGKQVYVNIVEVKQPDMDAQLVAEKIALDLENRISFRRAMKQAIGRAMRLGAKGIKTKVSGRLGGAEIARSESYHEGTIPLQTIRADIDYGTAEAHTTYGRLGVKVWIYKGEVLKGDAARAAEQREKVERKSRDNRNGDRRDDRRRRDNRNNGDRRNGGFRRGEKREGGKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVS3</accession>
    <name>R5VVS3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sortase (Surface protein transpeptidase)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00475</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000257">
        <property type="protein sequence ID" value="CCZ84622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00475"/>
    <dbReference type="EnsemblBacteria" id="CCZ84622">
        <property type="protein sequence ID" value="CCZ84622"/>
        <property type="gene ID" value="BN566_00475"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005754">
        <property type="entry name" value="Sortase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023365">
        <property type="entry name" value="Sortase_dom-sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04203">
        <property type="entry name" value="Sortase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63817">
        <property type="entry name" value="SSF63817"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01076">
        <property type="entry name" value="sortase_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="63"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="244" mass="27115" checksum="78C848FC9980C866" modified="2013-07-24" version="1">MLAGAVLLIAALSLVLWNWHESNQGGESAKKIRDTLHSALEEEEQNGNGFPIATTIVSIPEKQTTTDTQSVVGTVPKQDSESGSVSGVETSRPQIADAWDAYQTTTGTNPAKEQEIWAEIDSDWYIGILTIPTLQVEVPIFSEYVFDQLPYTPCRYAGSYLTDNMIVAAHNYDSHFGRIRKLDSGDRIDFTDVTGTVYHYQVIQTEILPDTAVEEMEQGNWDLTLFTCTLSGTQRVTVRCERIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVS8</accession>
    <name>R5VVS8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">DNA-directed RNA polymerase subunit omega</fullName>
            <shortName evidence="10">RNAP omega subunit</shortName>
            <ecNumber evidence="2 10">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">RNA polymerase omega subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9 10">Transcriptase subunit omega</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">rpoZ</name>
        <name evidence="11" type="ORF">BN566_00480</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Promotes RNA polymerase assembly. Latches the N- and C-terminal regions of the beta' subunit thereby facilitating its interaction with the beta and alpha subunits.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the RNA polymerase subunit omega family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="2 10"/>
    <dbReference type="EMBL" id="CBAR010000257">
        <property type="protein sequence ID" value="CCZ84627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00480"/>
    <dbReference type="EnsemblBacteria" id="CCZ84627">
        <property type="protein sequence ID" value="CCZ84627"/>
        <property type="gene ID" value="BN566_00480"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.940.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00366">
        <property type="entry name" value="RNApol_bact_RpoZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003716">
        <property type="entry name" value="DNA-dir_RNA_pol_omega"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006110">
        <property type="entry name" value="Pol_omega/Rpo6/RPB6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036161">
        <property type="entry name" value="RPB6/omega-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01192">
        <property type="entry name" value="RNA_pol_Rpb6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01409">
        <property type="entry name" value="RNA_pol_Rpb6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63562">
        <property type="entry name" value="SSF63562"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00690">
        <property type="entry name" value="rpoZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10 11" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="6 10" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ84627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="72" mass="8238" checksum="C01FEBFB0E565EE9" modified="2013-07-24" version="1">MSMLRPSLNQLMTKNESCYSVVVGIAKRAREIADELYDQKKELKEKPVQTAVDELAAGKYRIIESEELNHNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVZ2</accession>
    <name>R5VVZ2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00555</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000272">
        <property type="protein sequence ID" value="CCZ84702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00555"/>
    <dbReference type="EnsemblBacteria" id="CCZ84702">
        <property type="protein sequence ID" value="CCZ84702"/>
        <property type="gene ID" value="BN566_00555"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005235">
        <property type="entry name" value="YmdB-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36303">
        <property type="entry name" value="PTHR36303"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13277">
        <property type="entry name" value="YmdB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004789">
        <property type="entry name" value="DR1281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="68"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="39"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR004789-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR004789-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="254" mass="27972" checksum="9A9F777868625FCF" modified="2013-07-24" version="1">MHILLIGDVVGNSGCCFLQERLPQLKRTHHVDLTIVNGENSAQGNGITKHSMEQLFSAGADIITTGNHCFQRREALSIYEKETILRPLNFPDSCPGHGCTIWDNGPYQVAVINLMGTAYMDPLDNPFTVIEEFLPTLPTKNIIVDFHAEATAEKKAMGHFLTGKVSAVIGTHTHVQTADEAILNHHTGYLTDVGMTGGEYSILGVTVKPALDRFRFRFAQRFTEAPAPCFGNGVLLELDTSCGKCTKIERLIFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>R5UWU2</accession>
    <name>R5UWU2_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ADP-dependent (S)-NAD(P)H-hydrate dehydratase</fullName>
            <ecNumber evidence="6">4.2.1.136</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">ADP-dependent NAD(P)HX dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">nnrD</name>
        <name evidence="7" type="ORF">BN566_00598</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(6S)-NADHX + ADP = AMP + H(+) + NADH + phosphate</text>
            <dbReference type="Rhea" id="RHEA:32223"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.136"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate</text>
            <dbReference type="Rhea" id="RHEA:32235"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.136"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the NnrD/CARKD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.136" evidence="6"/>
    <dbReference type="EMBL" id="CBAR010000008">
        <property type="protein sequence ID" value="CCZ82793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00598"/>
    <dbReference type="EnsemblBacteria" id="CCZ82793">
        <property type="protein sequence ID" value="CCZ82793"/>
        <property type="gene ID" value="BN566_00598"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052855">
        <property type="term" value="F:ADP-dependent NAD(P)H-hydrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046496">
        <property type="term" value="P:nicotinamide nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01171">
        <property type="entry name" value="YXKO-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01965">
        <property type="entry name" value="NADHX_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017953">
        <property type="entry name" value="Carbohydrate_kinase_pred_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000631">
        <property type="entry name" value="CARKD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01256">
        <property type="entry name" value="Carb_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00196">
        <property type="entry name" value="yjeF_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01050">
        <property type="entry name" value="YJEF_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51383">
        <property type="entry name" value="YJEF_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 6" id="KW-0520">NAD</keyword>
    <keyword evidence="3 6" id="KW-0521">NADP</keyword>
    <keyword evidence="1 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="200"/>
            <end position="204"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ82793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="296" mass="31299" checksum="300F0211DE16DB2B" modified="2013-07-24" version="1">MQELSWKTICGYFPKRNRNSHKGTFGTLLCITGSNRMPGACALSTLAALRSGAGLVKVATTERNLPILASQIYEAVYLPMQTTETGEICWEKNAVVLQPALKQASAVLIGCGLGNTAATQELLRNVVNLVECPLIMDADGLNALASCIDIMQKQKPNWILTPHPGEMARLSGTTTAAVQANRPQCAAEFCKQFSGTLVLKGAGTIIQQGTTAIQNPTGNPGMSRGGSGDVLAGMIAAFAAQGLSPYDAACAGAYLHGLAGDFAAARYSEQAMLPQDLLHCLPEVFLKLEQERTAKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5UX78</accession>
    <name>R5UX78_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">YigZ family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00710</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IMPACT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000022">
        <property type="protein sequence ID" value="CCZ82908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00710"/>
    <dbReference type="EnsemblBacteria" id="CCZ82908">
        <property type="protein sequence ID" value="CCZ82908"/>
        <property type="gene ID" value="BN566_00710"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023582">
        <property type="entry name" value="Impact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015796">
        <property type="entry name" value="Impact_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001498">
        <property type="entry name" value="Impact_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036956">
        <property type="entry name" value="Impact_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015269">
        <property type="entry name" value="UPF0029_Impact_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020569">
        <property type="entry name" value="UPF0029_Impact_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16301">
        <property type="entry name" value="PTHR16301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09186">
        <property type="entry name" value="DUF1949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01205">
        <property type="entry name" value="UPF0029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="SSF54980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00257">
        <property type="entry name" value="IMPACT_YIGZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00910">
        <property type="entry name" value="UPF0029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UPF0029" evidence="2">
        <location>
            <begin position="17"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1949" evidence="3">
        <location>
            <begin position="138"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ82908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="217" mass="23488" checksum="CEAC36B1745CFC6C" modified="2013-07-24" version="1">MGYRTIAQAVTASFIEKKSEFIATLVPVQTADAAIACIEEVRKANRKARHNVYAYILRENHMTRYSDDGEPQGTAGVPVLNVLQKQNLTDVCCVVTRYFGGILLGGGGLVRAYSHSAALAVEAAQIQTKISCHPVTLKMAYSLYGKVSYQLPQLPILQQNADFGDTVTLSLLVPAGLTASFQADMIELTNGEISIQVGEQLDADFAEVADRLPPELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5UX82</accession>
    <name>R5UX82_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HD domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00713</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000026">
        <property type="protein sequence ID" value="CCZ82913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00713"/>
    <dbReference type="EnsemblBacteria" id="CCZ82913">
        <property type="protein sequence ID" value="CCZ82913"/>
        <property type="gene ID" value="BN566_00713"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="1">
        <location>
            <begin position="52"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="201" mass="23228" checksum="736B115DB370255B" modified="2013-07-24" version="1">MTHQETFIQIYQEHIHRRGADQLLAWLQSDASDFFTAPSSTRYHGSYEGGLVEHSLNVYHCLVDYLARPRTKELYGMDYSDETVALVALMHDICKVNFYVVDYRNAKNEQGVWEKVPYYTIRDEMPYGHGEKSVYMLSGFLRLSREEAFAIRYHMGFSGPEDRNQIGRALEMFPLALALNVADMEASYFLEGTPDKSGSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXH3</accession>
    <name>R5UXH3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GTP diphosphokinase</fullName>
            <ecNumber evidence="3">2.7.6.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_00575</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">In eubacteria ppGpp (guanosine 3'-diphosphate 5'-diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate</text>
            <dbReference type="Rhea" id="RHEA:22088"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:142410"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; ppGpp biosynthesis; ppGpp from GTP: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the relA/spoT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.5" evidence="3"/>
    <dbReference type="EMBL" id="CBAR010000007">
        <property type="protein sequence ID" value="CCZ82770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00575"/>
    <dbReference type="EnsemblBacteria" id="CCZ82770">
        <property type="protein sequence ID" value="CCZ82770"/>
        <property type="gene ID" value="BN566_00575"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00908">
        <property type="reaction ID" value="UER00884"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008728">
        <property type="term" value="F:GTP diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015970">
        <property type="term" value="P:guanosine tetraphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05399">
        <property type="entry name" value="NT_Rel-Spo_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01668">
        <property type="entry name" value="TGS_RSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004811">
        <property type="entry name" value="RelA/Spo_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045600">
        <property type="entry name" value="RelA/SpoT_AH_RIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007685">
        <property type="entry name" value="RelA_SpoT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033655">
        <property type="entry name" value="TGS_RelA/SpoT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13291">
        <property type="entry name" value="ACT_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19296">
        <property type="entry name" value="DUF5913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04607">
        <property type="entry name" value="RelA_SpoT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00954">
        <property type="entry name" value="RelA_SpoT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="SSF81271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00691">
        <property type="entry name" value="spoT_relA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="6">
        <location>
            <begin position="58"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="7">
        <location>
            <begin position="403"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="5">
        <location>
            <begin position="676"/>
            <end position="750"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ82770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="751" mass="86190" checksum="B5BF2B6917496CA8" modified="2013-07-24" version="1">MMNDQSVLQGIPDNVTISDLSKYIVRTNRNYDLPIIVSAYEFAFQAHGDQKRSSGEPYIVHPLSVAWILVSLGMDTECVCAGLLHDVVEDTGHTLDDICKLFGQNIAQLVDGVTKLVQMKQMVLNKEEQQMESVRKMLFAMSKDIRIIIIKLADRLHNMRTLHFLPPEKRSRISKETLNVYAPMADQLGIWKIKEELSDRAIMFLDPCGCYEIEKELQLKDEDAKNFIEKIQQELRKGLEKSTVIKNPPEVTGRVKSLYSIYRKVYAAIRKEDMHEISEVYDKYAARIIVDTVEECYVAMSVVGMLYQPLPNRTKDYIAMPKPNGYRSIHMTVYAYGLPFEVQIRTHAMHQAAEYGIVAHWKYKQGETSGNMDQWTNWARKLIEEQQNSDDMEEFVRNLKNDARQDVGILTPKGKPIFLPKGSTPIDFAYRIHTEVGNRMIGAKVNGKLVPLDQPLQIGQICEIECSKDPNKGPNRDWLNIVKTTEARSKIRSWLKKERREENIQTGRKTLENELRRNHIRMENDEKLTELLHDEMIRYKCETLDDFLASVGYCGINVSNLIPRLKDRYDKLYKKQEEEKPLSAENMPVYVQKKEDDSYVIVDQIDNLPNHCANCCHPVPGDEIVTFTTRGYGLAIHRADCPNYLRDLKSGDPERIGRWNKAKWTETAAAAPIVASLDVLAPDRIGLLMDVIKIVVDSRMMIVHSRSYNLKNGNAVCEITVRLNGNDQLQQLMDKLKRIPGVISVERTKKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXR3</accession>
    <name>R5UXR3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6017 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00890</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000059">
        <property type="protein sequence ID" value="CCZ83103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00890"/>
    <dbReference type="EnsemblBacteria" id="CCZ83103">
        <property type="protein sequence ID" value="CCZ83103"/>
        <property type="gene ID" value="BN566_00890"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046059">
        <property type="entry name" value="DUF6017"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19481">
        <property type="entry name" value="DUF6017"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6017" evidence="2">
        <location>
            <begin position="217"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="344" mass="39356" checksum="CC676575BF506E2E" modified="2013-07-24" version="1">MSAKKIKKVKTMPSSVCRVNKNANYTVMSNYHLRSTNLSLKAIGLLSKVLSLPENWDYSISGLTAICKEKETAIKAALDELKHWGYLTVTKLMPNETKSGRIEYVYDFYEYSEKDSPDADRDDDDTYDEDEETSAAEVNSIKKAPQGAEKQGIENLPLEILPVEIQEVENQGQINTKKIIKKNQILSDQVSINQSHSVSGKAVENDEKQTDGRIDGYICEKEIYTEVVKNNIDFSYFAEWLGDEEEAEEIVQMIVRRICSRKKTERICGQDFPREVVKSAMLKVDINVLENAIEQMKRTDNVRNYESYLISTLFNEANGKRFKENAEGRWAEYAVKRDFGGYDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5UY80</accession>
    <name>R5UY80_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">O-acetylhomoserine sulfhydrylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01064</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 3">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the trans-sulfuration enzymes family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01064"/>
    <dbReference type="EnsemblBacteria" id="CCZ83283">
        <property type="protein sequence ID" value="CCZ83283"/>
        <property type="gene ID" value="BN566_01064"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00614">
        <property type="entry name" value="CGS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001434">
        <property type="entry name" value="CGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="2">
        <location>
            <position position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR001434-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="410" mass="44393" checksum="D46D38A1A28DFC01" modified="2013-07-24" version="1">MEFNTALLHQQFDSDTQNGATIPPIYQVSAFAQSSAEQLEKVFNNKAAGFAYTRISNPTVDAFERRVASMEKGIGAVACSSGMAAVTMALLNILEAGDEVIAGSGLFGGTIDLFGDLKAFGITTHFVESVTAEAVAPLLNEKTKVVFTELIGNPKLDVVDLKKVSALVHEHGVPLIIDSTTATPYLLHTFDFGADVIVHSSSKYINGGGNSISGVIIDSGNFHWDPERYHSFAEYKKYGKFAYLAKLRNGIWRNFGSCLAPMNAFMNTVGLETLGLRMERICSNAKQLAEYLESRDDVTVNYPALKSSPYYDLVQTQFGGKGGAILTLRAGSKEKAFKLINALKYAVNATNIGDVRTLVIHPASTIYTHSSEQQKASAGVYDDSIRISVGIEDINDLIADFAQAIDQSKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYC7</accession>
    <name>R5UYC7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01109</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000087">
        <property type="protein sequence ID" value="CCZ83328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83328">
        <property type="protein sequence ID" value="CCZ83328"/>
        <property type="gene ID" value="BN566_01109"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="64" mass="7234" checksum="D0BD5BD8FBCDDEB3" modified="2013-07-24" version="1">MEFLFADYTQKTEALVTGIRLQNEAFDIYELQLLGLDKNNSCSYTNSGTATISDTELQELLHTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYH8</accession>
    <name>R5UYH8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH_17 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01149</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000093">
        <property type="protein sequence ID" value="CCZ83373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83373">
        <property type="protein sequence ID" value="CCZ83373"/>
        <property type="gene ID" value="BN566_01149"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041657">
        <property type="entry name" value="HTH_17"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12728">
        <property type="entry name" value="HTH_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH_17" evidence="1">
        <location>
            <begin position="21"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="72" mass="8267" checksum="032DEC3C1615F96F" modified="2013-07-24" version="1">MDSIPLEVVYKNMYSEYPEILEVKDLCEILGYGKKKVYQLIKEGQLKKIPCGRTIKVAKATIIDFVLQCAQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYT2</accession>
    <name>R5UYT2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Trypsin-like serine proteases typically periplasmic contain C-terminal PDZ domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01242</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000111">
        <property type="protein sequence ID" value="CCZ83468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01242"/>
    <dbReference type="EnsemblBacteria" id="CCZ83468">
        <property type="protein sequence ID" value="CCZ83468"/>
        <property type="gene ID" value="BN566_01242"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001940">
        <property type="entry name" value="Peptidase_S1C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13180">
        <property type="entry name" value="PDZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00834">
        <property type="entry name" value="PROTEASES2C"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="SSF50156"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="SSF50494"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="3">
        <location>
            <begin position="389"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="9"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="37"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="490" mass="52743" checksum="89D0F775C90B5684" modified="2013-07-24" version="1">MEEHPFSPIPQQQPAPQQQSPAPPPYTQVSSYAVPSPTPEQPQPAAQVPPPPVYPPNFGQPQPSAPYPPQQPIYPQMQPMQPMPAPKQKKRRTKQKPSPEQARLTKLAWIAIWLAFLLLVYCIASDLFRYFEMDGQTPTLQQSQSSTLNIQYQERPVSDAITQPDENGAYTVAGVAEAVAPSIVEITAASGSMPTSSGSGIILSEDGYIVTNAHVLQNSDTFWVTLNDSDEAISADCIGMDTKTDLAVLKINRTGLPAATIGDSDSLLVGEEVVAIGNPAGLTGTVTNGIVSALHRKIKSASTGFEMDCIQTNAAISPGNSGGALVNLYGQIVGITSSKYTNAYSGSAYEGLGFAISINAAMPILEELSSQGYVSGRVRIGITFRSLDITEVQEEFHETYQLSDRTQVSGLWISEIAEDCDISNTELQVGDVIERVNGTETADYDQLNAVLSDCKAGDSLTADCCRYDENGKKSSFTITFQLMEDRSGNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYV2</accession>
    <name>R5UYV2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphotransferase System HPr (HPr) Family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01252</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000116">
        <property type="protein sequence ID" value="CCZ83483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01252"/>
    <dbReference type="EnsemblBacteria" id="CCZ83483">
        <property type="protein sequence ID" value="CCZ83483"/>
        <property type="gene ID" value="BN566_01252"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00367">
        <property type="entry name" value="PTS-HPr_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1340.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000032">
        <property type="entry name" value="HPr-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035895">
        <property type="entry name" value="HPr-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00381">
        <property type="entry name" value="PTS-HPr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00107">
        <property type="entry name" value="PHOSPHOCPHPR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55594">
        <property type="entry name" value="SSF55594"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01003">
        <property type="entry name" value="PTS_HPr_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51350">
        <property type="entry name" value="PTS_HPR_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HPr" evidence="1">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="88" mass="9329" checksum="0FFC68B27262B11D" modified="2013-07-24" version="1">MVSKTVTVINEQGLHMRPAGILAKAVAAHKDCKVTLHANGKTIAAKAVMQIMAACMKKGCEVEIVCDGANEQAVLDEISKLFEDGFGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZ09</accession>
    <name>R5UZ09_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DNA sulfur modification protein DndD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01297</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000121">
        <property type="protein sequence ID" value="CCZ83528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01297"/>
    <dbReference type="EnsemblBacteria" id="CCZ83528">
        <property type="protein sequence ID" value="CCZ83528"/>
        <property type="gene ID" value="BN566_01297"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038729">
        <property type="entry name" value="Rad50/SbcC_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025662">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13476">
        <property type="entry name" value="AAA_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00675">
        <property type="entry name" value="SIGMA54_INTERACT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_23" evidence="2">
        <location>
            <begin position="6"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="264"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="346"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="500"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="638"/>
            <end position="743"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="931" mass="105871" checksum="E5D263BE08752CF0" modified="2013-07-24" version="1">MKPLHLELYGFGAFAEPCKISFTDWQDGEVFLITGETGAGKTTIFDAICFALYGTASGSERESRQFRSQYLTDSTAETKVRLEFLDNGKRYLVERNPAYERQKQRGIGTVEQKANATLYELLPNGEKTCICSGFKTVTCEITERIGMESTQFQQIVMIAQGEFRRFLLSNSIEKGQILSKLFHTESCGRIRDRLKQRYDAKTQEISMQTISIAAKLKTVAPHEATRKQQYQELLAEEKTVATSPICCQLLEEDCRERLEQLPLLEKEQERFQQSVDQLVQQMEQGRRHNQNCFRLEQQQKELQQLQQRQTVAAELLERAEINAVQIPEWQDAAAQLERSVPEYQHLADLQKELLILRKQSQQLQQEQAQKKATLEQIQQACAMLQKEVEEHAACAAELESTKTKMVQKQQALQVLQQLQQQFAAVRQAEADCAVLEQQAQQAHQQYYQVEKPKYDAMEQQFFQSMAGNLAAQLQAGAACPVCGSLEHPHPAEQTQQTVSEQQFQRARKQQEAALQAMQSQKSQLEKQQAVCAQLQTQKQQLLRQHKLSEQTTAETLEAMLKKIRSGISALTKQQKQLQEQLQQRTEQQAALKRKQEALPILQNAVTAQEQAVQALLQKITGKRTECDYVQNSLQYGTLQEAQQQLLCFRQKIEQAQLQQKRAQTQVQELEKQLAACQDACSRLEAEIAGKPLYDTAGAEQTLLEFRRQLSQKQREIAMQKADLQQVQETVAAVRLSLKTLEQASQTWMTYKELYSMMNGTNTEHGGERISLERYVQAYYFTRILEHANQKLDQLSNGRYQLIRRKEEDRKNISSGLNLNVLDQYTGTERDVKTLSGGETFLASLSLALGLSEAVQQQSGCVQINAMFIDEGFGSLDEMALENAIQLLQQLSGHDCMVGIISHVSALAERFDAQIRVEKTPCGSRAVCVGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZ46</accession>
    <name>R5UZ46_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Stage II sporulation protein E (SpoIIE)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01101</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01101"/>
    <dbReference type="EnsemblBacteria" id="CCZ83320">
        <property type="protein sequence ID" value="CCZ83320"/>
        <property type="gene ID" value="BN566_01101"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07228">
        <property type="entry name" value="SpoIIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="SSF81606"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="PPM-type phosphatase" evidence="2">
        <location>
            <begin position="420"/>
            <end position="624"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="629" mass="69641" checksum="C7B0460C4A10CAEF" modified="2013-07-24" version="1">MNGTAQDFLLYGMTACLTGTTAWFLHTVLLGLQNQQKIQLKSATGCAAAIVYILCIAALTSFDYPFMNFGRILGIAVTLLAARRFRYMGGVICGALTACGTMLCAGELGMPLLFLPITGLLSGYLVDRGIGLMTIAFFLINALAQLTVGLQVITFSSIGDLFLGCIAFLLLNNICFDRWLITANSSYDSTMQTISTRLQFMSEAIHSVREDTSEIAALLQKQKPEPVKEAVCDRICAGCKAQHHCWELQHTQTDAAFSEMERQPVSTLPEVPESLSDCLHTKQLQQQFYREKRRVQMETMQISRMDASRHILFEQLQATEDLIASVGDRMTVRYSTELTDRICCFLERYGYDFDYVIAYYTAKERLVVELYCKSRSIGSCMPAICHMLSESLGIALQELEPVRTRSTMRYRMCQAMRYQLEQYTISIPATEEAEMSGDTSIRFQDGTGCTYIVLSDGMGTGANAAIESKMTAEMFRKLICSGISDMAAVRLMNGLMVTKSAGEAFATLDAARVDLDEGTLTLLKAGAASTLIRQGNTILRVCAPTFPIGSTAVSDLYEKQILLSENDVFVMVSDGIHETEYTFIKELLLSTDDIRKMAEEICAKADIFSGGKHRDDITVTVVKLCRNAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZD5</accession>
    <name>R5UZD5_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">Histidinol-phosphatase</fullName>
            <shortName evidence="7">HolPase</shortName>
            <ecNumber evidence="4 7">3.1.3.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN566_01439</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>H2O + L-histidinol phosphate = L-histidinol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14465"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57699"/>
            <dbReference type="ChEBI" id="CHEBI:57980"/>
            <dbReference type="EC" id="3.1.3.15"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 8/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the PHP hydrolase family. HisK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.15" evidence="4 7"/>
    <dbReference type="EMBL" id="CBAR010000154">
        <property type="protein sequence ID" value="CCZ83703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01439"/>
    <dbReference type="EnsemblBacteria" id="CCZ83703">
        <property type="protein sequence ID" value="CCZ83703"/>
        <property type="gene ID" value="BN566_01439"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00013"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004401">
        <property type="term" value="F:histidinol-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010140">
        <property type="entry name" value="Histidinol_P_phosphatase_HisJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004013">
        <property type="entry name" value="PHP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003141">
        <property type="entry name" value="Pol/His_phosphatase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21039">
        <property type="entry name" value="PTHR21039"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02811">
        <property type="entry name" value="PHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00481">
        <property type="entry name" value="POLIIIAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="SSF89550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01856">
        <property type="entry name" value="hisJ_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="POLIIIAc" evidence="8">
        <location>
            <begin position="4"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ83703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="281" mass="31632" checksum="E36FCEEFD20AD7C3" modified="2013-07-24" version="1">MFGIDCHTHSGNSPDGTGTVGGLCRRAISLGLEALAITEHVELNRWHSQGYYGTTPRNDEEEYSYYKRMEKAMKDNHAAKEAYGNRLHIISGIELGQIPCDFGLADSVMQDNRLDFVIASLHELKNKPDFYFLDYRTEDVPKLLEQYFSELLTLCQWGKFDVLGHMTYPLRYIEGEAGIPVDLSAYEEQIHECLKALVNNGCGLELNTSGLRQKYGKPFPTLEYIKLYREMGGESLSIGSDAHCAEDVGSGIKEAIAIAKEAGFSYLCYYLEREPVYIPIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZE4</accession>
    <name>R5UZE4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">AICAR transformylase/IMP cyclohydrolase PurH (Only IMP cyclohydrolase domain in Aful)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01449</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000154">
        <property type="protein sequence ID" value="CCZ83713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01449"/>
    <dbReference type="EnsemblBacteria" id="CCZ83713">
        <property type="protein sequence ID" value="CCZ83713"/>
        <property type="gene ID" value="BN566_01449"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003937">
        <property type="term" value="F:IMP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004643">
        <property type="term" value="F:phosphoribosylaminoimidazolecarboxamide formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024051">
        <property type="entry name" value="AICAR_Tfase_dup_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024050">
        <property type="entry name" value="AICAR_Tfase_insert_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002695">
        <property type="entry name" value="PurH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11692">
        <property type="entry name" value="PTHR11692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01808">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00798">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="SSF53927"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="393" mass="43962" checksum="3AB31B0399C3FD0F" modified="2013-07-24" version="1">MASEMQLKYGCNPNQKPSRIFMADNSDLPITVLNGKPGYINLLDAFNGWQLVRELKQATGYCAATSFKHVSPAGAAIGKPLSDTLKKIYFVDDLGELSPLACAYARARGADRMSSYGDFIALSDVCDVPTAKMIQREVSDGIIAPGYEPEALEILKSKRKGTYNIIQIDPSYEPAALERKQVFGVTFEQGRNNLKIDESMLENIVTENKNLPEDKKHDLLISLITLKYTQSNSVCYVKDGQAIGIGAGQQSRIHCTRLAGNKADIWWLRQHPKVMGLQFVDGIRRPDRDNAIDVYISDEYMDVLADGAWENIFKVKPEVFTKEEQKAWLAQNTDVCLGSDAFFPFGDNIERAHKSGVTYIVEPGGSIRDDHVIMTCNKYHMVMAFCGIRFFHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZM2</accession>
    <name>R5UZM2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Hypothetical elongation factor G</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01234</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000109">
        <property type="protein sequence ID" value="CCZ83460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83460">
        <property type="protein sequence ID" value="CCZ83460"/>
        <property type="gene ID" value="BN566_01234"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="139" mass="16030" checksum="AAE2FFEF73EC4D54" modified="2013-07-24" version="1">MPRRVQQGDGLFRQGQDSLLGKDGNPSLPFQWVGIQKGIFMVDSALFADDTCLKQQCLRKRCFSGIYMGENANDGILIHIDSNPICFVVSFILCVKKGWFYYSIFYALRQCCAVYFVGTAHWNDYKIFVYCFDFRRCIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZT9</accession>
    <name>R5UZT9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01597</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000169">
        <property type="protein sequence ID" value="CCZ83868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83868">
        <property type="protein sequence ID" value="CCZ83868"/>
        <property type="gene ID" value="BN566_01597"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004394843" evidence="1">
        <location>
            <begin position="23"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="168" mass="18735" checksum="BDAB8A28A34FFE20" modified="2013-07-24" version="1">MKKVLGLVMAVAVLGGTLTGCGSSGGGSGKDGYSSAEKAAVAYWEAYYTLGTDKMMKLMPAEAKEHWEDELSLTSEQLTAYMEQELEKKSDIIKTFKSVSVDEQEELSIDRIQNFNENYLGEVGIKADEAVRCICTIYDDTGHIDDYRVTVYQYKGKWYSHSADAFFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5V001</accession>
    <name>R5V001_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01323</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000130">
        <property type="protein sequence ID" value="CCZ83555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83555">
        <property type="protein sequence ID" value="CCZ83555"/>
        <property type="gene ID" value="BN566_01323"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="143" mass="16325" checksum="9EF1C9D46C87EF39" modified="2013-07-24" version="1">MKQAEISVLNGLYKNAAVGKDAIFCVLEHTQDPDLRKELCTQLDYYETQQQKIRQQMKTFYQYPKELGTLAKVCSSASIRLHCLGGADNHQIAKLMVEGTNMGLIQLSQLMNRHANLTDSVRKQGAEMMQQEEAYLNRLKPYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>R5V033</accession>
    <name>R5V033_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 10 11">Ribulose-phosphate 3-epimerase</fullName>
            <ecNumber evidence="7 10 11">5.1.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">rpe</name>
        <name evidence="15" type="ORF">BN566_01363</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the reversible epimerization of D-ribulose 5-phosphate to D-xylulose 5-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10 11">
            <text>D-ribulose 5-phosphate = D-xylulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:13677"/>
            <dbReference type="ChEBI" id="CHEBI:57737"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="EC" id="5.1.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10 13">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="10 13">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Carbohydrate degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 10 11">Belongs to the ribulose-phosphate 3-epimerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.1" evidence="7 10 11"/>
    <dbReference type="EMBL" id="CBAR010000144">
        <property type="protein sequence ID" value="CCZ83600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01363"/>
    <dbReference type="EnsemblBacteria" id="CCZ83600">
        <property type="protein sequence ID" value="CCZ83600"/>
        <property type="gene ID" value="BN566_01363"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00113">
        <property type="reaction ID" value="UER00532"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004750">
        <property type="term" value="F:D-ribulose-phosphate 3-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019323">
        <property type="term" value="P:pentose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00429">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02227">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026019">
        <property type="entry name" value="Ribul_P_3_epim"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000056">
        <property type="entry name" value="Ribul_P_3_epim-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11749">
        <property type="entry name" value="PTHR11749"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00834">
        <property type="entry name" value="Ribul_P_3_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001461">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01163">
        <property type="entry name" value="rpe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01085">
        <property type="entry name" value="RIBUL_P_3_EPIMER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01086">
        <property type="entry name" value="RIBUL_P_3_EPIMER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="13" id="KW-0170">Cobalt</keyword>
    <keyword evidence="9 10 11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="13" id="KW-0464">Manganese</keyword>
    <keyword evidence="8 10 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="10 14">
        <location>
            <begin position="143"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="10">
        <location>
            <begin position="176"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="10 14">
        <location>
            <begin position="198"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10 12">
        <location>
            <position position="36"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10 12">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10 14">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 13">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 13">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 13 14">
        <location>
            <position position="67"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10 13">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR001461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR001461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001461-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CCZ83600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="219" mass="23846" checksum="9C9C4C0BB56B1B4D" modified="2013-07-24" version="1">MQKIQVSASILSADMCNLEAVVRTLETSGTDMLHFDVMDGRFVDNISFGIPVLEAIRKKTDLFLDVHLMISDPLHYAERFAKAGANGITFHLESESDPQKTIDAIHACGIPAGIVIKPNTPAEAVLPYLPSVEMVLVMTVEPGFGGQSFLWEMLKKVQFLRQKAAEQGLSVSIQVDGGVNAETAPEAVKAGANNLVVGSYLFRQENLKTAVDFFHQLAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5V038</accession>
    <name>R5V038_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Saccharopine dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01368</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000144">
        <property type="protein sequence ID" value="CCZ83605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01368"/>
    <dbReference type="EnsemblBacteria" id="CCZ83605">
        <property type="protein sequence ID" value="CCZ83605"/>
        <property type="gene ID" value="BN566_01368"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032095">
        <property type="entry name" value="Sacchrp_dh-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005097">
        <property type="entry name" value="Sacchrp_dh_NADP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16653">
        <property type="entry name" value="Sacchrp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03435">
        <property type="entry name" value="Sacchrp_dh_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sacchrp_dh_NADP" evidence="1">
        <location>
            <begin position="5"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Sacchrp_dh_C" evidence="2">
        <location>
            <begin position="142"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="401" mass="44796" checksum="CEF1D2EB39DD940F" modified="2013-07-24" version="1">MGKALIIGAGGVAGVVVHKCCQNSEVFTEICIASRTKSKCDALKAAVEAKGTKTVVTTAQVDANKVDELVALIEREKPDIVINVALPYQDLTIMDACLATKVDYVDTANYEPEDTAKFEYKWQWAYRERFEKAGITALLGSGFDPGVTGVFCAYAQKHYFDEIHEIDILDCNGGDHGYPFATNFNPEINIREVSAKGSYILDGKWVETEPMEIKRVYNFDEVGEKDMYLLHHEELESLALNIKGIHRIRFFMTFGQSYLTHLRCLENVGMTSIEPILYEGKEIVPLQFLKAVLPDPASLGPRTVGKTNIGCICQGVKDGKEVSYYVYNVCDHQECYKEVGSQAVSYTTGVPAMIGAMMVMTGKWKKPGVYNVEEFDPDPFMDALNKWGLPWKENFHPTLVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0F6</accession>
    <name>R5V0F6_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO</fullName>
            <ecNumber evidence="10">2.1.1.74</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Folate-dependent tRNA (uracil-5-)-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Folate-dependent tRNA(M-5-U54)-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">trmFO</name>
        <name evidence="12" type="ORF">BN566_01490</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + H(+) + NADH + uridine(54) in tRNA = (6S)-5,6,7,8-tetrahydrofolate + 5-methyluridine(54) in tRNA + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:16873"/>
            <dbReference type="Rhea" id="RHEA-COMP:10167"/>
            <dbReference type="Rhea" id="RHEA-COMP:10193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74447"/>
            <dbReference type="EC" id="2.1.1.74"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + H(+) + NADPH + uridine(54) in tRNA = (6S)-5,6,7,8-tetrahydrofolate + 5-methyluridine(54) in tRNA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:62372"/>
            <dbReference type="Rhea" id="RHEA-COMP:10167"/>
            <dbReference type="Rhea" id="RHEA-COMP:10193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74447"/>
            <dbReference type="EC" id="2.1.1.74"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the MnmG family. TrmFO subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.74" evidence="10"/>
    <dbReference type="EMBL" id="CBAR010000161">
        <property type="protein sequence ID" value="CCZ83755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01490"/>
    <dbReference type="EnsemblBacteria" id="CCZ83755">
        <property type="protein sequence ID" value="CCZ83755"/>
        <property type="gene ID" value="BN566_01490"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030698">
        <property type="term" value="F:5,10-methylenetetrahydrofolate-dependent tRNA (m5U54) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047151">
        <property type="term" value="F:methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase (FADH2-oxidizing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01037">
        <property type="entry name" value="TrmFO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002218">
        <property type="entry name" value="MnmG-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040131">
        <property type="entry name" value="MnmG_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004417">
        <property type="entry name" value="TrmFO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11806">
        <property type="entry name" value="PTHR11806"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11806:SF2">
        <property type="entry name" value="PTHR11806:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01134">
        <property type="entry name" value="GIDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00137">
        <property type="entry name" value="gid_trmFO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 10" id="KW-0274">FAD</keyword>
    <keyword evidence="4 10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9 10" id="KW-0520">NAD</keyword>
    <keyword evidence="8 10" id="KW-0521">NADP</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="GIDA" evidence="11">
        <location>
            <begin position="8"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="12"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CCZ83755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="448" mass="49334" checksum="B3429950FA2E1F36" modified="2013-07-24" version="1">MTEQRKQAIVIGGGLAGCEAAWQLAQNGIAVTLYEQKPVHYSPAHHRNGLAELVCSNSLKADRLASAAGLLKAEMERMGSLLIPCARKTSVPAGGALAVDRDAFSDLVTEQIRSHPLITLETATIDEIPAGNVVVATGPLTEGKLAEQIEQLCGTRLRFFDAAAPIVKYESLDAEKVFFAARYGRGTADYINCPMNQEEYEAFYEALVNAERAELHDCDKNFFRVYEGCMPIEILASRGKDTMRFGPLKPVGLTDPRTGRRPYAVIQLRRENQEGTMYNIVGFQTNLKFGEQKRVFSMIPGLEHAEFVRYGVMHRNSFLDSPRLLGADYALRSNPNCFFAGQMTGVEGYIESAASGILAGQNLARRMQGKAPLILPRETMMGALAAYISDETVAQFQPMGCNMGILPELPERIRDKREKYQAYADRALAALDAYLQQTAENTTEGEGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0H6</accession>
    <name>R5V0H6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01814</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000186">
        <property type="protein sequence ID" value="CCZ84088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84088">
        <property type="protein sequence ID" value="CCZ84088"/>
        <property type="gene ID" value="BN566_01814"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="291" mass="33473" checksum="65D1098008E1C9BF" modified="2013-07-24" version="1">MEANQTQTAAFADLSLSAGTIAELQTYLLAVANYYHILPIEKLYSILCEQNPEFLSKEDFLSFLQAQKTQPTCYQILKPGELFEGVPANLNLLWNGMLVHECLLVDQDECYAFLEFSEGKPYHVPEKELLLQYATEEFYEETPEVTAMRAVFADMGMSPARQESELKNYILFAQENAELPEVFDDIQRRKLTFNDRTLRKFVRAYRNLHNHLPLPLIHGCTPVELLEVFPEMPGEVLYWTEDVYKTPEVQKRMEQLVSAAATQPKQSLADFMQSQIDLKKMLEDVPGQMPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0P6</accession>
    <name>R5V0P6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN566_01893</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000193">
        <property type="protein sequence ID" value="CCZ84168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01893"/>
    <dbReference type="EnsemblBacteria" id="CCZ84168">
        <property type="protein sequence ID" value="CCZ84168"/>
        <property type="gene ID" value="BN566_01893"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015114">
        <property type="term" value="F:phosphate ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005436">
        <property type="term" value="F:sodium:phosphate symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044341">
        <property type="term" value="P:sodium-dependent phosphate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003841">
        <property type="entry name" value="Na/Pi_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004633">
        <property type="entry name" value="NaPi_cotrn-rel/YqeW-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030147">
        <property type="entry name" value="NptA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026022">
        <property type="entry name" value="PhoU_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10010">
        <property type="entry name" value="PTHR10010"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10010:SF46">
        <property type="entry name" value="PTHR10010:SF46"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02690">
        <property type="entry name" value="Na_Pi_cotrans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01895">
        <property type="entry name" value="PhoU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00704">
        <property type="entry name" value="NaPi_cotrn_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="50"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="85"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="121"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="186"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="252"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="286"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="PhoU" evidence="8">
        <location>
            <begin position="356"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="PhoU" evidence="8">
        <location>
            <begin position="464"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ84168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="601" mass="66275" checksum="04B6B304247DFDBC" modified="2013-07-24" version="1">MDIFSFLTLFGGLAFFLYGMNVMSSGLEKLAGGKLEVILQKITSNKWKSLLFGVGITAAVQSSSAVTVMLVGLVNSGIMQLGQTIGILMGSNIGTTLTVWLLALSGISGESVFLKLIKPESFSPVLALIGIIMIMTAKHNKTKDIGRILVGFSVLFTGMMMMGESVEPLKDSPSFSHILTAFNNPLVGVLAGAIITAVIQSSAASIGILQSLSITGHITYGMAIPIIMGQNIGTCVTALISSIGVNRSARRVAVIHIAFNCIGTIILLPILYLVNAFVDFVFMDWTISYAGIALVHTIFNVVVTFLLLPFSKLLEKLAYLVIKQLPEETQKEQLILPGEILLNTPAVAIEACRTSVNDMARLAEDTILKALSLHDNYSESVAHAVSKQEKLLDQYEDKLNAYIVRISKHSIASSDNRTVSKMLHGIGNFERIGDHALNVAQSAQEMHDKQISFSTEAKSELQAMRAALTETVHLAFDAFCKDDLNLAHQVEPMEEVIDTFNMRMKKKHIERLQKAACTVELGYIFQDLLTNFERISDHCSNIAGCLIEIEENRDMHEYLHDLKKSDESFQEQYHANLERYMMQLQQFMEQNQQTTEPVKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0Q0</accession>
    <name>R5V0Q0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01898</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000193">
        <property type="protein sequence ID" value="CCZ84173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01898"/>
    <dbReference type="EnsemblBacteria" id="CCZ84173">
        <property type="protein sequence ID" value="CCZ84173"/>
        <property type="gene ID" value="BN566_01898"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="352" mass="41016" checksum="C20AD6F5778238F4" modified="2013-07-24" version="1">MQHYTFPYTEVFHCTAGDCPDSCCKGWEIVIDPKTLQTYQQTCSPIGETIRSTLTKNEDDEWMFQNREDGNCPFWNAEKLCDLQIQAGEHLLCDTCRQFPRIPQDYTLFTEHVLSLACPEAARLVLTQTEPFQAIPLTQAEPSEPVNEAELPYSLNWMNCLLETRAVLLQLFQETSIPLAEALCYGLHYVQQFQDWIEEKTDDFPELQPIPSKPVFLHHESVQHLYDTLEWMTPEWADWMQQIQQHPVTDADLQAFAKVAEPWIPAFRNFCSYYLYLYWLQALNTGNALIQYQKLLTALHEIAIVSAWKLRETHTYTLTDCMIIVQRYAKEVMHDAENIEKIEDVLYNLGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0S1</accession>
    <name>R5V0S1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01923</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84198">
        <property type="protein sequence ID" value="CCZ84198"/>
        <property type="gene ID" value="BN566_01923"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="79" mass="8770" checksum="3A3AA4D8EDBE6D92" modified="2013-07-24" version="1">MWVRTQNEDALYHVAAVRAVKMISERTYSVIGILLGASTSDSVVLGEYKDYATAKAEVDHMDALLMEGTSSVYHMTKEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0W1</accession>
    <name>R5V0W1_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Cell division protein FtsZ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ftsZ</name>
        <name evidence="9" type="ORF">BN566_01639</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 5">Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer. Polymerizes to form a dynamic ring structure in a strictly GTP-dependent manner. Interacts directly with several other division proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="4">Assembles at midcell at the inner surface of the cytoplasmic membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the FtsZ family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000172">
        <property type="protein sequence ID" value="CCZ83910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01639"/>
    <dbReference type="EnsemblBacteria" id="CCZ83910">
        <property type="protein sequence ID" value="CCZ83910"/>
        <property type="gene ID" value="BN566_01639"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000917">
        <property type="term" value="P:division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043093">
        <property type="term" value="P:FtsZ-dependent cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051258">
        <property type="term" value="P:protein polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02201">
        <property type="entry name" value="FtsZ_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00909">
        <property type="entry name" value="FtsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000158">
        <property type="entry name" value="Cell_div_FtsZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020805">
        <property type="entry name" value="Cell_div_FtsZ_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045061">
        <property type="entry name" value="FtsZ/CetZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024757">
        <property type="entry name" value="FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30314">
        <property type="entry name" value="PTHR30314"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12327">
        <property type="entry name" value="FtsZ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00423">
        <property type="entry name" value="CELLDVISFTSZ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="SSF52490"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="SSF55307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00065">
        <property type="entry name" value="ftsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01135">
        <property type="entry name" value="FTSZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 5 9" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 4 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 4 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0717">Septation</keyword>
    <feature type="domain" description="Tubulin" evidence="7">
        <location>
            <begin position="15"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin_C" evidence="8">
        <location>
            <begin position="209"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="319"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="319"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="23"/>
            <end position="27"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="110"/>
            <end position="112"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ83910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="380" mass="40112" checksum="790563B4623C561E" modified="2013-07-24" version="1">MTDFIYEDAFEPDVNIKVIGVGGGGGNALNCMVASGVQDIEYIAINTDAKALNNSKATTRIQIGAKLTKGRGAGNKPAIGQQSAEENKEEIEAALKGADMVFITAGMGGGTGTGAAPVVAKIAQEAGILTVAVVTKPFAFEREQKMAQAEKGIMELKKYVDSLIVIPNEKLLEGMDKPLTMRESFALADDILKTGVKSISDLIVEEGYINLDFADVSTIMKGAGYAHLAIGHGSGKDKAKEAANAVIASPLLETSIAGAKRLLINITMSEDILSSDVDNATKMITDKAADNVEFIFGTAFKEDMQDEMTITVIAAGFDEETDDSEEAEEPAAEEAAAPEEPEAQPQPQPQPQQTQAAANGGKKPADPDDLMAIFEILDRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5V157</accession>
    <name>R5V157_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Aspartate carbamoyltransferase</fullName>
            <ecNumber evidence="3">2.1.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN566_00236</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>carbamoyl phosphate + L-aspartate = H(+) + N-carbamoyl-L-aspartate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20013"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:32814"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="EC" id="2.1.3.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the aspartate/ornithine carbamoyltransferase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.2" evidence="3"/>
    <dbReference type="EMBL" id="CBAR010000213">
        <property type="protein sequence ID" value="CCZ84323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00236"/>
    <dbReference type="EnsemblBacteria" id="CCZ84323">
        <property type="protein sequence ID" value="CCZ84323"/>
        <property type="gene ID" value="BN566_00236"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00116"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004070">
        <property type="term" value="F:aspartate carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:cellular amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002082">
        <property type="entry name" value="Asp_carbamoyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020542">
        <property type="entry name" value="Asp_carbamoyltrfase_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036792">
        <property type="entry name" value="Asp_carbatrfase_reg_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405:SF16">
        <property type="entry name" value="PTHR11405:SF16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02748">
        <property type="entry name" value="PyrI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="SSF53671"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57825">
        <property type="entry name" value="SSF57825"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00670">
        <property type="entry name" value="asp_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00097">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="OTCace_N" evidence="8">
        <location>
            <begin position="8"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="OTCace" evidence="7">
        <location>
            <begin position="155"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="PyrI_C" evidence="9">
        <location>
            <begin position="326"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ84323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="365" mass="40730" checksum="24D75D4F7D4DB1F3" modified="2013-07-24" version="1">MTTSKHPHLIDLNDYPVSWWKEVVDLGEKIKNNPEVYSGACHNKIMATLFYEPSTRTQMSFQTAMLRLGGRLIGFDNPANSSVSKGETLKDTTKIVSNYADILVMRHPVAGAAKAAALSADCPVINAGDSGHLHPTQTLTDLLTLKCEKGRLDHLTIGLCGDLINGRTVHSLIKAMTCFEGNQFILISTPELKLPSYVKDILTASGCPFREVASLEEVLPELDVLYMTRIQQERFASREAYLAQKDTYILTVKKLKAAKPDMIILHPLPRVDEIEVAVDEDPRAMYFNQAWYGMYVRMALIITMLDLNKKDNPTILLRGTTVKGAACKNPNCITCSESYLPKSFLELGNGLLECEYCNERLLLER</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1K0</accession>
    <name>R5V1K0_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ribonuclease J</fullName>
            <shortName evidence="9">RNase J</shortName>
            <ecNumber evidence="9">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">rnj</name>
        <name evidence="13" type="ORF">BN566_01865</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">An RNase that has 5'-3' exonuclease and possibly endonuclease activity. Involved in maturation of rRNA and in some organisms also mRNA maturation and/or decay.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer, may be a subunit of the RNA degradosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. Bacterial RNase J subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="9"/>
    <dbReference type="EMBL" id="CBAR010000192">
        <property type="protein sequence ID" value="CCZ84140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01865"/>
    <dbReference type="EnsemblBacteria" id="CCZ84140">
        <property type="protein sequence ID" value="CCZ84140"/>
        <property type="gene ID" value="BN566_01865"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' exoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01491">
        <property type="entry name" value="RNase_J_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011108">
        <property type="entry name" value="RMMBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004613">
        <property type="entry name" value="RNase_J"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042173">
        <property type="entry name" value="RNase_J_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030854">
        <property type="entry name" value="RNase_J_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041636">
        <property type="entry name" value="RNase_J_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07521">
        <property type="entry name" value="RMMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17770">
        <property type="entry name" value="RNase_J_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004803">
        <property type="entry name" value="RnjA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00649">
        <property type="entry name" value="MG423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7 9" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="9" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Lactamase_B" evidence="12">
        <location>
            <begin position="80"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="9 10">
        <location>
            <begin position="422"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR004803-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CCZ84140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="613" mass="67517" checksum="67A7674FFE89F2B6" modified="2013-07-24" version="1">MATKEGKEGKAAALPKKRNYRRRTPKQNSGEQPAKQARGRAKTVTRAAAFSAAQSRSNHKQEVRRTPVRIIPLGGLNEIGKNMTVFECSNDMFILDCGLAFPDGDMPGVDIVIPDFTYVEANRDKVRGIVLTHGHEDHIGGLAYLLKKINVPVYGTKLTLGLVKGKLEEHGLANSVKLCEVQPRRTVKMGCMGVEFIKVNHSIPGAVGMAIHTPAGIIVHTGDFKVDYSPVDDDIIDLARFGELGSRGVLALMADSTNAERSGFTRSERTVGESFDKLFKQATGKRIIIATFSSNIHRIQQIVDNAQRSNRKVAVFGRSMVNVVNIALEMGYLKIPKGMLIDIDEMNRFPDERVVLITTGSQGEPMSALTRMAMNSHKKVVITPNDFIIISANPIPGNEKHVTRVVNELMRSGAEVIYEDVHVSGHACQEELKLIHALTKPKFFIPVHGEYKHLVKHAQIAYSMGMPESNVIIGSIGDVIETDGVSMKIVTQVPSGRVMVDGLGVGDVGSIVLRDRKHLAQDGLIIVVIGIERTANLIVSGPDIISRGFVYVRESEELMEEARQILLDTLQHCSAQELKEWGTLKNKLRDVLSDYIYGKTKRSPMILPIIMEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1L9</accession>
    <name>R5V1L9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative agmatine deiminase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00352</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000231">
        <property type="protein sequence ID" value="CCZ84453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00352"/>
    <dbReference type="EnsemblBacteria" id="CCZ84453">
        <property type="protein sequence ID" value="CCZ84453"/>
        <property type="gene ID" value="BN566_00352"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004668">
        <property type="term" value="F:protein-arginine deiminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009446">
        <property type="term" value="P:putrescine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007466">
        <property type="entry name" value="Peptidyl-Arg-deiminase_porph"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31377">
        <property type="entry name" value="PTHR31377"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04371">
        <property type="entry name" value="PAD_porph"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="79" mass="8607" checksum="0D77111CFD1B5D6D" modified="2013-07-24" version="1">MDLWDGEPTRTPGERLAAYVNFDISNGVVIVPQFNDPMDAVAVSILQDCFPTRTVVPIAAREILIGGGNIHCITQQIPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1M9</accession>
    <name>R5V1M9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01905</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01905"/>
    <dbReference type="EnsemblBacteria" id="CCZ84180">
        <property type="protein sequence ID" value="CCZ84180"/>
        <property type="gene ID" value="BN566_01905"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025641">
        <property type="entry name" value="DUF4340"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14238">
        <property type="entry name" value="DUF4340"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4340" evidence="1">
        <location>
            <begin position="77"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4340" evidence="1">
        <location>
            <begin position="252"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="467" mass="52708" checksum="9473B63BEBB6E21D" modified="2013-07-24" version="1">MKKQVIVILSLVLLVAITLGAYLFVRNNNQEKQKKESEDAAALQLVDFNSSNINKIEIQTPDGDDYTAVIGAESNVWELTEDTDFHINVYFLNSVAGSLCNLTATENLGVATEDIKKQYELDNPYTITLSTDDGSRTLYTGKLTATKEYYYIMSDKSDDVFLVEAKYGDYLRPNKNSLKSIYVQTNQNSQIDQMKLIHNGETVFDLKKKDATSWKMVSPIESDFVNVLNINNLQTNIMQLIIDKFGEEHVTEDQYAEYGFDDPAYTFYFSQEDGTETTIYAKDYDTESAQFVECLQKETGQIFYCAANYISFLLAEPADFLSDVIYDADMADVASVQVHIDGETDLNMELNSENKQYTVNDIDVAALGTDAVDALTTLYDNLTGLTTSTLDLTDNVPTEEEPEITLTYNLKDGTTEVITFLAKDKESYYVLRNGEYSGFLAKRTAFTSRNGILEFYHRLEKTIGMTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5V214</accession>
    <name>R5V214_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00434</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000247">
        <property type="protein sequence ID" value="CCZ84573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84573">
        <property type="protein sequence ID" value="CCZ84573"/>
        <property type="gene ID" value="BN566_00434"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="168"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="43"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="202" mass="23571" checksum="F2A8911DAE946F4D" modified="2013-07-24" version="1">MEEHTEQKRVMADTYMEEVWEQQAVKNWSKPMHNFGVRQNAFNQSADQRIRALESENLQLRTQIRTLMQQTEELSQIAQRQKERLRQEEQRNREIFGRVAAMLDRMRNDLDRVRLSTQLNSNAIDRLMLEDEAEPEAVEEPLTTMEEPAEEVLETLEEAETVETVAAVESIEPEAEPEQVTESIDEPVAPETTIKPKDSYVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5V220</accession>
    <name>R5V220_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00041</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000219">
        <property type="protein sequence ID" value="CCZ84360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84360">
        <property type="protein sequence ID" value="CCZ84360"/>
        <property type="gene ID" value="BN566_00041"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="111" mass="12504" checksum="CD49DC401D24A7CF" modified="2013-07-24" version="1">MLAADEKQTALQTIQAVVTAIAQNHYAAIPTLLSRSDLPMEELQGEIERVLRFQKTTVAPFSEAFADFYEDEDGEGFEIDYRLTESLILTFEGFQREGTLQIVLEDVTAEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>R5V267</accession>
    <name>R5V267_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Primosomal protein N'</fullName>
            <ecNumber evidence="10">3.6.4.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">ATP-dependent helicase PriA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">priA</name>
        <name evidence="12" type="ORF">BN566_00481</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Involved in the restart of stalled replication forks. Recognizes and binds the arrested nascent DNA chain at stalled replication forks. It can open the DNA duplex, via its helicase activity, and promote assembly of the primosome and loading of the major replicative helicase DnaB onto DNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the primosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the helicase family. PriA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.-" evidence="10"/>
    <dbReference type="EMBL" id="CBAR010000257">
        <property type="protein sequence ID" value="CCZ84628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00481"/>
    <dbReference type="EnsemblBacteria" id="CCZ84628">
        <property type="protein sequence ID" value="CCZ84628"/>
        <property type="gene ID" value="BN566_00481"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006268">
        <property type="term" value="P:DNA unwinding involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1440.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00983">
        <property type="entry name" value="PriA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005259">
        <property type="entry name" value="PriA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041222">
        <property type="entry name" value="PriA_3primeBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042115">
        <property type="entry name" value="PriA_3primeBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041236">
        <property type="entry name" value="PriA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040498">
        <property type="entry name" value="PriA_CRR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17764">
        <property type="entry name" value="PriA_3primeBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18074">
        <property type="entry name" value="PriA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18319">
        <property type="entry name" value="PriA_CRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00595">
        <property type="entry name" value="priA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 10" id="KW-0235">DNA replication</keyword>
    <keyword evidence="9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 10" id="KW-0347">Helicase</keyword>
    <keyword evidence="6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 10" id="KW-0639">Primosome</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="226"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="10">
        <location>
            <begin position="454"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="10">
        <location>
            <begin position="481"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CCZ84628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="754" mass="84685" checksum="0AB3A13422FAD654" modified="2013-07-24" version="1">MVLECQEPPAAETAVKPVLAVLDALPVLQAEQLDLVFWLREMTFCTYCDAIRTILPAGMQLQIVQKTVLVQPKPNVRLSDEEERYYQILNTASTPAAFQKRLHHPLAANLLEKGLLQQVSAAKSRVQESKVKLLSLADPLPERKMTPKQQSLVKLLQECGTLPEKEACRRCGITKAVVQSAEKNGLLVCEMRVAEPIAEDVPVTESLEQVVLTEEQESVMEQVLQKVLAEETAYFLLHGVTGSGKTRVFQKLIAETLKQGKQAILLVPEIALTPQIVGQFQSLFGNAVVVMHSALTDRQRQDAYQRMQNGTAKIAIGTRSAVFAPVQNLGILIVDEEGERTYKSENAPRYHAIAVARKRCQTHHCPLLLASATPSIESYYYAKKGVYTLLKLKQRYRNMPLPEVTVVDMNRERQLGNGTPFSNTLLDAMQETLSQGKQVLLLLNRRGYHTMISCCSCNEPVYCPNCSVPMTYHKVSDQLMCHYCGHMQPMPETCPHCGGKQFRRMGFGTQQMEEQLQLLFPKANVLRMDADTTGSRYAYEEKFAAFRNKEYEIMVGTQMIGKGLDFPDVTLVGVLSLDQALFAGDFRSYERTFSLITQVVGRSGRGDTAGRAILQTFLPNHYVLQLAAQQNYPVFYEQEYAIRKTMLFPPFCDLCILGFVGEKEEIVAAAAAEAVRQIQVYVKEQAFSYPLWVLGAVPCHYERLKGKYRYRVILKCKNTSPYRTLIRAVLEAVTAQKAFAKLYLYADMNGDIGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5V276</accession>
    <name>R5V276_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ATP synthase subunit delta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">ATP synthase F(1) sector subunit delta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">F-type ATPase subunit delta</fullName>
            <shortName evidence="6">F-ATPase subunit delta</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">atpH</name>
        <name evidence="7" type="ORF">BN566_00327</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation.</text>
    </comment>
    <comment type="function">
        <text evidence="6">This protein is part of the stalk that links CF(0) to CF(1). It either transmits conformational changes from CF(0) to CF(1) or is implicated in proton conduction.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ATPase delta chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000225">
        <property type="protein sequence ID" value="CCZ84420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00327"/>
    <dbReference type="EnsemblBacteria" id="CCZ84420">
        <property type="protein sequence ID" value="CCZ84420"/>
        <property type="gene ID" value="BN566_00327"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045261">
        <property type="term" value="C:proton-transporting ATP synthase complex, catalytic core F(1)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01416">
        <property type="entry name" value="ATP_synth_delta_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026015">
        <property type="entry name" value="ATP_synth_OSCP/delta_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000711">
        <property type="entry name" value="ATPase_OSCP/dsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11910">
        <property type="entry name" value="PTHR11910"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00213">
        <property type="entry name" value="OSCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00125">
        <property type="entry name" value="ATPASEDELTA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47928">
        <property type="entry name" value="SSF47928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01145">
        <property type="entry name" value="ATP_synt_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0139">CF(1)</keyword>
    <keyword evidence="2 6" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="3 6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ84420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="167" mass="18693" checksum="85D5012249982F3F" modified="2013-07-24" version="1">MSRPAECYAQAMLELQYPMDVVEQAQAIWECCPALSEALTNPTISIPEKEAVVRKVFPEAVQNLLCVLCKNAETADVPEIFQAFYEKKRQSESCVHAVVEYVTPLTEAQQEGLKQFAMKVTGKQQAVLTMQKNPALRGGFVLRIGDLVYDRSLRNTLKTLQKKLAGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>R5V2H1</accession>
    <name>R5V2H1_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha</fullName>
            <shortName evidence="10">ACCase subunit alpha</shortName>
            <shortName evidence="10">Acetyl-CoA carboxylase carboxyltransferase subunit alpha</shortName>
            <ecNumber evidence="10">2.1.3.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">accA</name>
        <name evidence="12" type="ORF">BN566_00394</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the acetyl coenzyme A carboxylase (ACC) complex. First, biotin carboxylase catalyzes the carboxylation of biotin on its carrier protein (BCCP) and then the CO(2) group is transferred by the carboxyltransferase to acetyl-CoA to form malonyl-CoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>acetyl-CoA + N(6)-carboxybiotinyl-L-lysyl-[protein] = malonyl-CoA + N(6)-biotinyl-L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:54728"/>
            <dbReference type="Rhea" id="RHEA-COMP:10505"/>
            <dbReference type="Rhea" id="RHEA-COMP:10506"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:83144"/>
            <dbReference type="ChEBI" id="CHEBI:83145"/>
            <dbReference type="EC" id="2.1.3.15"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Acetyl-CoA carboxylase is a heterohexamer composed of biotin carboxyl carrier protein (AccB), biotin carboxylase (AccC) and two subunits each of ACCase subunit alpha (AccA) and ACCase subunit beta (AccD).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the AccA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.15" evidence="10"/>
    <dbReference type="EMBL" id="CBAR010000240">
        <property type="protein sequence ID" value="CCZ84495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00394"/>
    <dbReference type="EnsemblBacteria" id="CCZ84495">
        <property type="protein sequence ID" value="CCZ84495"/>
        <property type="gene ID" value="BN566_00394"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00655">
        <property type="reaction ID" value="UER00711"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009317">
        <property type="term" value="C:acetyl-CoA carboxylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003989">
        <property type="term" value="F:acetyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016743">
        <property type="term" value="F:carboxyl- or carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001295">
        <property type="term" value="P:malonyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00823">
        <property type="entry name" value="AcetylCoA_CT_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001095">
        <property type="entry name" value="Acetyl_CoA_COase_a_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011763">
        <property type="entry name" value="COA_CT_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42853">
        <property type="entry name" value="PTHR42853"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03255">
        <property type="entry name" value="ACCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01069">
        <property type="entry name" value="ACCCTRFRASEA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00513">
        <property type="entry name" value="accA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50989">
        <property type="entry name" value="COA_CT_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6 10" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="3 10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CoA carboxyltransferase C-terminal" evidence="11">
        <location>
            <begin position="1"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CCZ84495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="285" mass="31507" checksum="989F628FB66451EB" modified="2013-07-24" version="1">MSSEKQRTAWEKQMLVREKNRPTIHDYLPRIFEEFFELHGDRGYGDDGAILGGIARLNGVPVTIIAEVKGRNLEENKRSNFAMPHPEGYRKALRLAKQAEKFHRPIICFIDTPGAFCGVEAEERGQGEAIAKNLAEFMSLKTPVVSVVLGEGGSGGALALGVCDDLAMLENALYSVISPRGAASILWKDASREQEACEIMRITAQDLLEFGVADAIIPEPEDGAQGDLDKMSQNIKEYLVKTIAVKSKKDIDILQKERYTKFRKIGVFSEPSHEVQKAAAGNENE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>R5V2N8</accession>
    <name>R5V2N8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Translation initiation factor IF-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">infA</name>
        <name evidence="5" type="ORF">BN566_00081</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">One of the essential components for the initiation of protein synthesis. Stabilizes the binding of IF-2 and IF-3 on the 30S subunit to which N-formylmethionyl-tRNA(fMet) subsequently binds. Helps modulate mRNA selection, yielding the 30S pre-initiation complex (PIC). Upon addition of the 50S ribosomal subunit IF-1, IF-2 and IF-3 are released leaving the mature 70S translation initiation complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the 30S ribosomal translation pre-initiation complex which assembles on the 30S ribosome in the order IF-2 and IF-3, IF-1 and N-formylmethionyl-tRNA(fMet); mRNA recruitment can occur at any time during PIC assembly.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IF-1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000241">
        <property type="protein sequence ID" value="CCZ84535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00081"/>
    <dbReference type="EnsemblBacteria" id="CCZ84535">
        <property type="protein sequence ID" value="CCZ84535"/>
        <property type="gene ID" value="BN566_00081"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04451">
        <property type="entry name" value="S1_IF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00075">
        <property type="entry name" value="IF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006196">
        <property type="entry name" value="RNA-binding_domain_S1_IF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004368">
        <property type="entry name" value="TIF_IF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33370">
        <property type="entry name" value="PTHR33370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01176">
        <property type="entry name" value="eIF-1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00008">
        <property type="entry name" value="infA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50832">
        <property type="entry name" value="S1_IF1_TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 2 3" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="2 3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="S1-like" evidence="4">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ84535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="72" mass="8270" checksum="8AE6912ACC7FA987" modified="2013-07-24" version="1">MSKEDMIEVEGVVLESLPNAMFKVELQNKHVILAHVSGRLRMNYIRIVPGDKVTVEMSPYDLTKGRITWRAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5V2R7</accession>
    <name>R5V2R7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00426</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000246">
        <property type="protein sequence ID" value="CCZ84565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84565">
        <property type="protein sequence ID" value="CCZ84565"/>
        <property type="gene ID" value="BN566_00426"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="284" mass="31190" checksum="C2F647A010DEC8FA" modified="2013-07-24" version="1">MQQDWKAIWDNLNPDEAAKERMKQAILQKIQAETPQTASKKPPFFLQRCSFGTFARAAALCCMTVTVALAGLHLTDRRTAELLPEPSVSEQPAETTTPHTLPAQTTATLQTQTTTKTKTQTALQTTATAKQTVTSLVMTEQTVASSAAAIEKPDAGIEEPSKEPIQTEPICTEPEPVLTTTAAETSAIEAPETTTTTIFRGVETYGSLFDFQYARWNGVHYQTDYSAVSYEDLDDLAGFSVAIQSESDRVYTILVYPLKGYSIQDCIAVQYAGDSDYFYFYAIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>R5V3T3</accession>
    <name>R5V3T3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transcription-repair-coupling factor</fullName>
            <shortName evidence="9">TRCF</shortName>
            <ecNumber evidence="9">3.6.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">mfd</name>
        <name evidence="12" type="ORF">BN566_00097</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Couples transcription and DNA repair by recognizing RNA polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent release of RNAP and its truncated transcript from the DNA, and recruitment of nucleotide excision repair machinery to the damaged site.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">In the C-terminal section; belongs to the helicase family. RecG subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">In the N-terminal section; belongs to the UvrB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.-" evidence="9"/>
    <dbReference type="EMBL" id="CBAR010000002">
        <property type="protein sequence ID" value="CCZ82754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00097"/>
    <dbReference type="EnsemblBacteria" id="CCZ82754">
        <property type="protein sequence ID" value="CCZ82754"/>
        <property type="gene ID" value="BN566_00097"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000716">
        <property type="term" value="P:transcription-coupled nucleotide-excision repair, DNA damage recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00969">
        <property type="entry name" value="TRCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036101">
        <property type="entry name" value="CarD-like/TRCF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003711">
        <property type="entry name" value="CarD-like/TRCF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004576">
        <property type="entry name" value="Mfd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037235">
        <property type="entry name" value="TRCF-like_C_D7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005118">
        <property type="entry name" value="TRCF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041471">
        <property type="entry name" value="UvrB_inter"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02559">
        <property type="entry name" value="CarD_CdnL_TRCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03461">
        <property type="entry name" value="TRCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17757">
        <property type="entry name" value="UvrB_inter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01058">
        <property type="entry name" value="CarD_TRCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00982">
        <property type="entry name" value="TRCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141259">
        <property type="entry name" value="SSF141259"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143517">
        <property type="entry name" value="SSF143517"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00580">
        <property type="entry name" value="mfd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8 9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="628"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="810"/>
            <end position="964"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CCZ82754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="1157" mass="130839" checksum="AB04701B5F3ABA4C" modified="2013-07-24" version="1">MPFFETTMQQMQAFRQLQRARQEGCSPVALTGCAMVQKAQLALTLSSPEQPVLILTGEEAEARRLCADINTMTNDPQTALLFPSKELVFAPAEGISTAYVHARLGVLSALQQKTCSVVAASIEALMQPVIPPEQLQQESLTLKNGDTIPMDTLRKKLISMGYQHCEAVEGAGQFSIRGAIVDIFSAQAPQPYRMEFWDEEVDTISFFDPQTQRRTESVSEMMLIPAKETLCPPEELAERIRSHAKTLRGKYAAVAKEHLLQEAQQLEDGLDLVQIDKYYPLIHETPALLTDYGFSTVLLSEYSSIAETARALLSRHREDCKVLLEDGVLCRGLDGYYQEPEVLQERLSKYFCVYANQFLQGGTHVAYEKLLSMEWVQLSPWGGELRQLIEDLKSYLEQHSTIFLLAGSEKTLPILQNDLQESGIPCKLAEGETEWLPDTVYLRTGSLSGGYYCPETNFVVMTQGRTMRAGSRKRRIHKEGAALHSLSDLTPGDLVVHATHGIGRFLGIRKLELEGMTKDYITIQYRGNENLYVPVTQMDLVSRYISGRDDSGVKLNRLSSVEWQKTRNNVKHAVKDMAKELTELYAKREKSQGFAFYPDSREQTDFEARFPYIETDDQLQSIAEIKADMERPRPMDRLLCGDVGFGKTEVALRAAMKCILSGKQCVILVPTTVLAMQHYQTALRRFETFPVNIVLLSRYNSPKQTKENLKKIRSGVADLVIGTHRVVQKDVQFKALGLAIVDEEQRFGVAHKEKFKEMFPGVDMLTLSATPIPRTLNMAMSGIRDMSVIAEPPQDRYPVQTCVMEYHLGVLVQAMRRELKRGGQVYYIHNRVETMPQTAAKLQEVMPEARIAIANGQMDEKELTEIWRQLVEYEIDILICTTIIETGVDVPNVNTLIIERADCFGLSQLYQLRGRVGRSNRRGYAYFTFRRDQVLSEEATKRLTAMREFTQFGSGFQIAMRDLEIRGAGSLLGGRQHGHMEAVGYEMYLQLLNEAIAEEKGEPIQKTAECVVDVQMEAHIPETYIESLSQRLDIYRKIAAITTEEDQSELLDELIDRYGEPPKAILGLLTVALLRNRAAQLGITEITQRNQTLYFYIEHPTQEQITALACAYRGKIVFNGMQKPYIGVKLEPKRLPLEQMQDVLAIMMQANQKPDET</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5V430</accession>
    <name>R5V430_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Precorrin-4 C11-methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_00676</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the precorrin methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000020">
        <property type="protein sequence ID" value="CCZ82874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00676"/>
    <dbReference type="EnsemblBacteria" id="CCZ82874">
        <property type="protein sequence ID" value="CCZ82874"/>
        <property type="gene ID" value="BN566_00676"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046026">
        <property type="term" value="F:precorrin-4 C11-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11641">
        <property type="entry name" value="Precorrin-4_C11-MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006362">
        <property type="entry name" value="Cbl_synth_CobM/CibF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="SSF53790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01465">
        <property type="entry name" value="cobM_cbiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TP_methylase" evidence="5">
        <location>
            <begin position="1"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ82874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="251" mass="27147" checksum="186374FD51EC9E10" modified="2013-07-24" version="1">MVYFVGAGAGDPELLTIKGKRLIDHADVLIYAGSLVNPEVLADRKPESVVYDSASMTLEEVLAVMKQAEQAGKTTVRVHTGDASIYGAIREQLDALDRMGIAHEVVPGVSSFLAAAAALQKEYTLPDVSQTVILTRMAGRTPVPEREQIESLAAHQATMVIFLSVGQIAELVQRLSTSYPLQTPVAVVYKASWPDQKLVTGTLKTIAEQVQAAGIQKTALVLVGEFLGDTYALSKLYDKTFTHEYRKGIES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5V4A3</accession>
    <name>R5V4A3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00744</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000030">
        <property type="protein sequence ID" value="CCZ82944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82944">
        <property type="protein sequence ID" value="CCZ82944"/>
        <property type="gene ID" value="BN566_00744"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="348" mass="38476" checksum="5B5C331D2294E770" modified="2013-07-24" version="1">MIAQYRIASDKEVCPYVYVMADLDLDGYPELMVKTGNCEANYAYEIWTKGADGKAVKAGTANGFHAHLYYNDQNGTLYSNYYHQGVQEIMSYVLKSDTLQQTADYSWKQNYDSGHGSPTEMKNVYLLEETDINHYVFQNSSRESGTGEFTSLEEFIAAAQGINSNQNQSEEDKHLVTDTPQQQETTPPAAVASEDTLKEQVLSKCGNSPLDWYYDDFDGDGNKEAFAMAYRDNGMGGHEILGLFYVNAQGTVTDLGTDFWGACGAGRVVAYNSSKKFFVFDTNNGGSGSTDYLYGVTNGACYSSNLSGSLHGFQVDENGAYTFEGGSAAHERPQIRLYYDESTGDFHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5V4C8</accession>
    <name>R5V4C8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00764</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000032">
        <property type="protein sequence ID" value="CCZ82964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00764"/>
    <dbReference type="EnsemblBacteria" id="CCZ82964">
        <property type="protein sequence ID" value="CCZ82964"/>
        <property type="gene ID" value="BN566_00764"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ82964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="312" mass="36479" checksum="4F647210C85DC7BC" modified="2013-07-24" version="1">MTKEKTVEIACKIETPADLEDLDHLQCAYQAKNDFTDFFRSLYEESLQFLPHRNIHRIYYGSEFCEFRIPAISECKAVLQKVNQKKLPVTFLTPPVTQTGIEHIKALLPILEAHSCEISVNDYGVLQLLHDHHYHGNIICGRILDKLYHDGRMNRFAFSQYTNEMGYQYLRSPAVSAPSFQEALKKYHVTRYDIDLPQYGLNLPENQNNISYSTFLPYGYITTGRICMMRNYQKDQFDGFDLTKNTCHKVCRQYDQLLYQPVGTIQMNSNHMRNRSVSIMRKGNTLFYGCTDIIPEELQQFDRIILQPKLML</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>R5V4K4</accession>
    <name>R5V4K4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HTH lysR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00797</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000038">
        <property type="protein sequence ID" value="CCZ83004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00797"/>
    <dbReference type="EnsemblBacteria" id="CCZ83004">
        <property type="protein sequence ID" value="CCZ83004"/>
        <property type="gene ID" value="BN566_00797"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="290" mass="33125" checksum="733D7DB3D09206D4" modified="2013-07-24" version="1">MDFHQLQYFVAIAESGSITAAAKKLFLSQPPLSMQMKRLEKELGCVLFLRGAREVQLTEAGKLLYTRAKSMLELRRITEEELAVCSEQTSGTIRLGVISSVGCSLLPQWLKWFHAQYPQIRFSLYESDTYQILEKLHQDRVELALVRSPFSAEGIQKVPLQTESLIAVGHHSFFSKADTISLQELAERPLIIYRRWEHILNQTFRTLELPVSYFCVNEDARTTVYWADAGLGVGIIPESACSLLQNPETRIVPISDSLPDTTIYLVRREQAYLAPHTKIFWEGLQKHCKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>R5V4U9</accession>
    <name>R5V4U9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative tRNA (cytidine(34)-2'-O)-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.207</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA (cytidine/uridine-2'-O-)-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00921</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Could methylate the ribose at the nucleotide 34 wobble position in tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-carboxymethylaminomethyluridine(34) in tRNA(Leu) + S-adenosyl-L-methionine = 5-carboxymethylaminomethyl-2'-O-methyluridine(34) in tRNA(Leu) + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:43088"/>
            <dbReference type="Rhea" id="RHEA-COMP:10333"/>
            <dbReference type="Rhea" id="RHEA-COMP:10334"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74508"/>
            <dbReference type="ChEBI" id="CHEBI:74511"/>
            <dbReference type="EC" id="2.1.1.207"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>cytidine(34) in tRNA + S-adenosyl-L-methionine = 2'-O-methylcytidine(34) in tRNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:43084"/>
            <dbReference type="Rhea" id="RHEA-COMP:10331"/>
            <dbReference type="Rhea" id="RHEA-COMP:10332"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74495"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.207"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family. TrmL subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.207" evidence="1"/>
    <dbReference type="EMBL" id="CBAR010000062">
        <property type="protein sequence ID" value="CCZ83134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00921"/>
    <dbReference type="EnsemblBacteria" id="CCZ83134">
        <property type="protein sequence ID" value="CCZ83134"/>
        <property type="gene ID" value="BN566_00921"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18094">
        <property type="entry name" value="SpoU-like_TrmL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01885">
        <property type="entry name" value="tRNA_methyltr_TrmL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016914">
        <property type="entry name" value="tRNA_cyt/urid_MeTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42971">
        <property type="entry name" value="PTHR42971"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029256">
        <property type="entry name" value="SpoU_TrmH_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00185">
        <property type="entry name" value="tRNA_yibK_trmL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="SpoU_methylase" evidence="3">
        <location>
            <begin position="4"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1 2">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 2">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 2">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR029256-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="170" mass="19477" checksum="E0BF3AC3B8253FFD" modified="2013-07-24" version="1">MKQLKIVLVEPQIPQNTGNIARTCAVTGASLHLVQPMGFTVTDKHLKRAGLDYWHKLDITYYDDWADFVAKHADEQDQFYFFTTKGKHVHSDAIYTELDSVYIIFGREDKGLPEPLLHAHANQCVRIPMRHTLRSLNLSNSVAIATYEVLRQWGYPELQCASELDVCEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5V502</accession>
    <name>R5V502_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00982</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000070">
        <property type="protein sequence ID" value="CCZ83199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83199">
        <property type="protein sequence ID" value="CCZ83199"/>
        <property type="gene ID" value="BN566_00982"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="56" mass="6610" checksum="C33F5A9E094079C6" modified="2013-07-24" version="1">MKKKKSSAQGLPEYTGRYPKPKLRFRLRVVFFIFLSCLLIGLVFYLLGVNFDLFSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5V5D3</accession>
    <name>R5V5D3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00126</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000090">
        <property type="protein sequence ID" value="CCZ83344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00126"/>
    <dbReference type="EnsemblBacteria" id="CCZ83344">
        <property type="protein sequence ID" value="CCZ83344"/>
        <property type="gene ID" value="BN566_00126"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032531">
        <property type="entry name" value="DUF4956"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16316">
        <property type="entry name" value="DUF4956"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="252" mass="27670" checksum="E4FC72190596F370" modified="2013-07-24" version="1">MFNSVLSSVGLSVGAAATTDTTSFLSNTVTTATDAKTIVIILFLGVLVGLLISLLYIITHRKNGYSQSYVMTLLILPPIVAIVLSMINSTASALSLAGVFTLCRYRTIPGDPKDITYIFFAMAAGVLCGIEGAGHIWLIFLFYIVVAAVLLAVEFSGYGKCKTSSMTLKITIPEDMNYTGLFDEILNQNTTSWKLRRVKTTNFGSLFELVYSVEMKNTVDQKAFIDQIRTLNGNLTVVLTLFRYDDKVYETN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-09-29" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5V5H5</accession>
    <name>R5V5H5_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SMI1_KNR4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01160</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000093">
        <property type="protein sequence ID" value="CCZ83384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83384">
        <property type="protein sequence ID" value="CCZ83384"/>
        <property type="gene ID" value="BN566_01160"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018958">
        <property type="entry name" value="Knr4/Smi1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037883">
        <property type="entry name" value="Knr4/Smi1-like_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00860">
        <property type="entry name" value="SMI1_KNR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160631">
        <property type="entry name" value="SSF160631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMI1_KNR4" evidence="1">
        <location>
            <begin position="23"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="155" mass="17824" checksum="5CFDAF289ED77F65" modified="2013-07-24" version="1">MAFFENIEIQKLFCAEEADTFPPFTEKDLAETEKAMGWKLPASYLALLNIKNGGRIDEAYEESWLSVIYGISSNGKGLADWYDNWINEWQYPNIGIPFGETQSAGHDLYFMDYRAVDENGEPRIVLIDNEMNNAVTVVAKNLEEFLTKIYHHEEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5V5Z8</accession>
    <name>R5V5Z8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">von Willebrand factor type A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01283</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000119">
        <property type="protein sequence ID" value="CCZ83514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83514">
        <property type="protein sequence ID" value="CCZ83514"/>
        <property type="gene ID" value="BN566_01283"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="SSF56436"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="161"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="302" mass="33352" checksum="509524A02BC6D801" modified="2013-07-24" version="1">MDSYKQCPNCGKTFPAQDAFCDDCGCQLDWMPSTQPQQSFMPPPTQTTTAYYAEKPKKTGTLIGLLVAILLILAAMIVLGFFLIHQLNQNDTTNHTSELAAVAQTETTEMAAETEPPAAETAAPEIETKISVVTVIEQVTVPQTDSPSVYAVPTPEPDPQPAGKTYQAFVDACTWADAKQKCEAAGGHLAYITSDEDWNQAISVLSGTGLRYVWLGGSTYISNDGSTITASWLNGSNLNYIDNARHWYPNEPSGWDNSSPEHSLEPYILLWYVDDYWSLNDTSNDILKYYKPERIGYLCEFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5V6E4</accession>
    <name>R5V6E4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein serine/threonine phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01435</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000154">
        <property type="protein sequence ID" value="CCZ83699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01435"/>
    <dbReference type="EnsemblBacteria" id="CCZ83699">
        <property type="protein sequence ID" value="CCZ83699"/>
        <property type="gene ID" value="BN566_01435"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006470">
        <property type="term" value="P:protein dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PTHR13832"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="SSF81606"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="1">
        <location>
            <begin position="46"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="319" mass="35730" checksum="BD5DBDADCB177F5B" modified="2013-07-24" version="1">MKFDTNSDMEQEEEALYYYSSNKNEIFQTQDDTLFIKPGITAYNLRVSVSTHIGKVRGNHEDNFYLEGLRLNEIFRNDFSATYTISDADGMSFAVFDGMGGAAYGEIASEIAVQTMKKYERRLKNAEGMRAVDQIVTEFVTEANNSICDMLREKNCLSGGTTFAMLYFIDGLAKLYYLGDSRIYMQDEEDLICLTRDHTLANQKLDAGIYTEAQAKKSADQHRLTLYVGVDKNGLGLNADSRPPVALQPGRKFLLCTDGLVDMCSEQEIQDILSQDYFNEAAVLVQTAVNYGGIDNVTCIVLEVIPIEEPAELESDPML</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>R5V6G5</accession>
    <name>R5V6G5_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HTH asnC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_01455</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000155">
        <property type="protein sequence ID" value="CCZ83719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01455"/>
    <dbReference type="EnsemblBacteria" id="CCZ83719">
        <property type="protein sequence ID" value="CCZ83719"/>
        <property type="gene ID" value="BN566_01455"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13404">
        <property type="entry name" value="HTH_AsnC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="147" mass="16397" checksum="415B2913382B03D9" modified="2013-07-24" version="1">MDKIDAMLITLLQENARAPLKVLASKVFLSAPAVSSRIDKLEKQGIILGYNTIIDRQKLGYHITAFINLELSADQKAEFYPFINSCPNVLECNCVTGVYSMLIKVSFPSTQELDTFIGKIQRFGNTSTQIVFSTPVPHREISVETNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5V6J0</accession>
    <name>R5V6J0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01474</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000160">
        <property type="protein sequence ID" value="CCZ83739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83739">
        <property type="protein sequence ID" value="CCZ83739"/>
        <property type="gene ID" value="BN566_01474"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="107" mass="12203" checksum="48E73424646A31B8" modified="2013-07-24" version="1">MNPESFSSLPEGQWLLQQVQTAFPNCSADEQLDGVAILCTIRQTIGHPPLFRECILFFQQHPQHPWERWMLPFLCMNVSEWQTFWAAVEAVLSASACTESTKSCSKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5V6X1</accession>
    <name>R5V6X1_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphoesterase</fullName>
            <ecNumber evidence="3">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_01589</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the metallophosphoesterase superfamily. YfcE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="3"/>
    <dbReference type="EMBL" id="CBAR010000167">
        <property type="protein sequence ID" value="CCZ83854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01589"/>
    <dbReference type="EnsemblBacteria" id="CCZ83854">
        <property type="protein sequence ID" value="CCZ83854"/>
        <property type="gene ID" value="BN566_01589"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110165">
        <property type="term" value="C:cellular anatomical entity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024654">
        <property type="entry name" value="Calcineurin-like_PHP_lpxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000979">
        <property type="entry name" value="Phosphodiesterase_MJ0936/Vps29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11124">
        <property type="entry name" value="PTHR11124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12850">
        <property type="entry name" value="Metallophos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00040">
        <property type="entry name" value="yfcE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos_2" evidence="4">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="161" mass="18011" checksum="95AB8BD94813E5C1" modified="2013-07-24" version="1">MRIIVLSDTHGNPKVLETILARHADADACIHLGDGEGDVKLFLSCHPEWESKFYAVRGNCDFNSSLPISRTIDLIPGHRIFAVHGHRHAIRYTPDILVEEAIAEDCNIVLHGHTHVRRNEYRNGVYLMNPGSAAQPRDGMPPSYAYIDYEKGGIFISHVSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-09-29" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7K9</accession>
    <name>R5V7K9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01805</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000186">
        <property type="protein sequence ID" value="CCZ84079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84079">
        <property type="protein sequence ID" value="CCZ84079"/>
        <property type="gene ID" value="BN566_01805"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16323">
        <property type="entry name" value="Syd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1580.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037883">
        <property type="entry name" value="Knr4/Smi1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009948">
        <property type="entry name" value="Syd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038228">
        <property type="entry name" value="Syd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07348">
        <property type="entry name" value="Syd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160631">
        <property type="entry name" value="SSF160631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="189" mass="21738" checksum="8024FE7E5F6E1BC2" modified="2013-07-24" version="1">MNMKKAFEKFFEEMTQNYLNEWGVPPRVPYTEKNPTGLFLPETLNDKGYAQWKPKLQEKPVSFDAMEKELGFSIHPQIKAFLSTYWFLALDGIIQTSRGEAELRLNGLTPYSNLGKHIIFAFDNKETHYLHNQKYFLIGTFCSIGGNDSYLVQVNNDTGEVTAVDVMNKRSVHLADSIEELLCNMKGIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7M6</accession>
    <name>R5V7M6_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Stage 0 sporulation protein A homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_01830</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000187">
        <property type="protein sequence ID" value="CCZ84104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01830"/>
    <dbReference type="EnsemblBacteria" id="CCZ84104">
        <property type="protein sequence ID" value="CCZ84104"/>
        <property type="gene ID" value="BN566_01830"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0010">Activator</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="7">
        <location>
            <begin position="2"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="6">
        <location>
            <begin position="141"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="5">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ84104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="209" mass="23280" checksum="04B9274AF2CC1ADF" modified="2013-07-24" version="1">MRIILIDDDQLVTLSLKTILESTGSVQVLATGTSGAEAIALYQQWKPDVLLLDIQMPNGSGLDAGEQILQQDPNARILFLTTFSDDAYIAKALELGAKGYLLKQDFAGIVPALEAVMQGQHVFGEQVVSCFSKQFKKKSTFDYAKYDITEKEQEIIAQVAKGYSNKEIAAALCFSEGTIRNYLSSILEKLQLRDRTQLAVYYYHHQMDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7Q7</accession>
    <name>R5V7Q7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">LysM domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01864</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000192">
        <property type="protein sequence ID" value="CCZ84139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01864"/>
    <dbReference type="EnsemblBacteria" id="CCZ84139">
        <property type="protein sequence ID" value="CCZ84139"/>
        <property type="gene ID" value="BN566_01864"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00118">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024300">
        <property type="entry name" value="DUF3794"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12673">
        <property type="entry name" value="DUF3794"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3794" evidence="1">
        <location>
            <begin position="38"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3794" evidence="1">
        <location>
            <begin position="187"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="517" mass="57566" checksum="4AC5B238DC86F4FF" modified="2013-07-24" version="1">MEWKINRERIPVTERILDETQEQGVELDYVLPDYDPEIFRIIRCEMQPAILEYQLQETRLSYELQVDLRILYCGSDSHRLQCVTQTMHYTKTLDLPEKPENPAVLLQAKADYANCRAVSRRRLDVRGAVSVRVQITGERMQEVISDVFGMQVQCQKQTIAYASQKQRAVKTISLSEDIERNAAKAPIQSILRCTARAEQGEQSIIAGKFVVRGEVLVSLLYCSTSEEQTEPESMQFTLPYSQIIDMEQIDETFTGMAAVQVIRCECKPVSNKGSDSDNLHCEADLRITCTACKPATISIVTDAYSTAYLCDCVRTPLRIDGTPVLLKEQFSCTAAIPAGDTPVDFVSDLQCRIKNVSATPVPEQNRIRVSGMLCCSVLAKDQEGMPFLLEKEEAFEELLATTVPLEQAMLCAEAEPADSSFHLAADGSLSIKATISLQGMLYPATQQTGLSEITVDENRKRTRDSDCALRLYYGTVGESVWEIAKRCNTKVSAIAAENDLSGDTLTKPGMLFIPIVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5V840</accession>
    <name>R5V840_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribosome hibernation promoting factor</fullName>
            <shortName evidence="2">HPF</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">hpf</name>
        <name evidence="4" type="ORF">BN566_00212</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Required for dimerization of active 70S ribosomes into 100S ribosomes in stationary phase; 100S ribosomes are translationally inactive and sometimes present during exponential growth.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with 100S ribosomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HPF/YfiA ribosome-associated protein family. Long HPF subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000209">
        <property type="protein sequence ID" value="CCZ84299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00212"/>
    <dbReference type="EnsemblBacteria" id="CCZ84299">
        <property type="protein sequence ID" value="CCZ84299"/>
        <property type="gene ID" value="BN566_00212"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044238">
        <property type="term" value="P:primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00552">
        <property type="entry name" value="RaiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00839">
        <property type="entry name" value="HPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034694">
        <property type="entry name" value="HPF_long/plastid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036567">
        <property type="entry name" value="RHF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003489">
        <property type="entry name" value="RHF/RaiA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032528">
        <property type="entry name" value="Ribosom_S30AE_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038416">
        <property type="entry name" value="Ribosom_S30AE_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16321">
        <property type="entry name" value="Ribosom_S30AE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02482">
        <property type="entry name" value="Ribosomal_S30AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69754">
        <property type="entry name" value="SSF69754"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00741">
        <property type="entry name" value="yfiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="Ribosom_S30AE_C" evidence="3">
        <location>
            <begin position="114"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="175" mass="20285" checksum="CE07975B92442CD0" modified="2013-07-24" version="1">MKMTITAKKMQIPQNFTEYAEKRLNSKLDKFFGEEADAKITMATHKNLIVIELTVDYNNMKYRAEQSAVDKEDALDAAIDKIIRQIRRNKTRVEKKLKDSAFKDVAYEDVVPESDYEVIRHKKFVMHPMDVEEAILQMNLLGHTFFMFLNANTGETNVVYKRADGKYAVLEPVAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5V8E2</accession>
    <name>R5V8E2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Abortive infection protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00296</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000222">
        <property type="protein sequence ID" value="CCZ84389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00296"/>
    <dbReference type="EnsemblBacteria" id="CCZ84389">
        <property type="protein sequence ID" value="CCZ84389"/>
        <property type="gene ID" value="BN566_00296"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071586">
        <property type="term" value="P:CAAX-box protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="204"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="222" mass="25539" checksum="0C9B4E3B4C52B53F" modified="2013-07-24" version="1">MTHSKKQKAITLMVYFLLFYAVWAVYHVWIAPFLTAHISSDWLCSLVANGIFKNLIWTLPAAILLCRFSGDVYATPKECFSWNRNQLPWLLVFPFFAVYLLFGAYRLHGGLQILPDFGLSSVIVVLFVGLTEELVFRGWLLNLTYCESHKGVAIGINAVLFLMIHFPKWMMDGTFFSNFAHFGFISILLLSVLFSVIFVKTKSLLLPIGLHMFWDLLMMMVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>R5V918</accession>
    <name>R5V918_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional accessory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00455</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000251">
        <property type="protein sequence ID" value="CCZ84594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00455"/>
    <dbReference type="EnsemblBacteria" id="CCZ84594">
        <property type="protein sequence ID" value="CCZ84594"/>
        <property type="gene ID" value="BN566_00455"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05685">
        <property type="entry name" value="S1_Tex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3500.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041692">
        <property type="entry name" value="HHH_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044146">
        <property type="entry name" value="S1_Tex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023323">
        <property type="entry name" value="Tex-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032639">
        <property type="entry name" value="Tex_YqgF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037027">
        <property type="entry name" value="YqgF/RNaseH-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17674">
        <property type="entry name" value="HHH_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16921">
        <property type="entry name" value="Tex_YqgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="SSF47781"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S1 motif" evidence="1">
        <location>
            <begin position="260"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="334" mass="36015" checksum="AFDD3654B937E1DA" modified="2013-07-24" version="1">MAALLKTLPYPAAYMVVSEAGASVYSASKLAAEEFPEYDVSLRSAVSIARRLQDPLAELVKIDPQAIGVGQYQHDMPKKELTAALDGVVEDCVNGVGVDLNTASFSLLSHVAGINSTIAKNIVAYRTENGAFTDRKQLKKVSKLGAKTFEQCAGFLRIPNAANPLDQTAVHPESYAAAEAILNACGFSLSAMTPQTHPEILAAADRYGLDALAKQAGVGVPTMNDILQELSKPGRDPRESLPPPLLRSGDIMELKDLKPGMELMGTVRNITDFGCFVDIGVHEDGLVHISKICKHYIKHPMEVVQVGEVVKVWVLEVDGKRGRISLTMIPPEKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>R5VGT2</accession>
    <name>R5VGT2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pyridine nucleotide-disulfide oxidoreductase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00591</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000008">
        <property type="protein sequence ID" value="CCZ82786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00591"/>
    <dbReference type="EnsemblBacteria" id="CCZ82786">
        <property type="protein sequence ID" value="CCZ82786"/>
        <property type="gene ID" value="BN566_00591"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="424"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="528" mass="56518" checksum="9350BEEE66969B36" modified="2013-07-24" version="1">MKRHELIVIGAGPAGLSAAIEAKKNGMDVIVFDENQRPGGQLFKQIHKFFGSKEHHAKVRGFRIGQQLLEEAEGLGVRVELDATVMGIYENKEVTVKKDGEVHHYKGDAIIIATGAAENMIPFKGWNIPGVIGAGAAQTMMNIQGVQPGQKILMVGSGNVGLVVSFQLLQAGCEVVAIIDAAPRVGGYGVHAAKLTRTGVPFYLSHTVVEAKGTDRVTGAVIAQVDDHFQPIPGTEKELDVDTICIAVGLTPMSQLASNATCNMELIPQKGGHVALLSEYGETSVSGIYCAGDVAGIEEASSAMIQGRSVASHVSMKAGYLTEAEFEEKYTGYQEALGQLRQGMFAPKNKGRNDFTETDEGYPISKTLLAHGYMTEEELAAFPAASYQKPGIHPVIECTQNIPCNPCQDACKFGCIKVGANITRLPAIDEEKKCTGCGLCVASCSGQAIFLVDETYEEGYASIAFPYEFLPMPKIGDKGTALDRQGKPVCEAEIVGVKRAPIMDKTAVVTMKVPIAFVKTARFYRPLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5VHS6</accession>
    <name>R5VHS6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">RNAse M5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00844</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000043">
        <property type="protein sequence ID" value="CCZ83051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00844"/>
    <dbReference type="EnsemblBacteria" id="CCZ83051">
        <property type="protein sequence ID" value="CCZ83051"/>
        <property type="gene ID" value="BN566_00844"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025156">
        <property type="entry name" value="RNase_M5_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13331">
        <property type="entry name" value="DUF4093"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4093" evidence="1">
        <location>
            <begin position="95"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="188" mass="21116" checksum="CD9A2343BA7EEE15" modified="2013-07-24" version="1">MVEGKYDKIKLSSIVDAVILVTNGFQIYKNPEQLDLIRYYARTTGVILMTDADRAGFQIRNYLKGAIQQGTVYQVYTPDVYGKEHRKEKPSAEGKLGVEGIRKELLLAALERAGVLAEETAPKNPEEQITAYDLYTVGLNGQADSSDRRRALQKQLALPARMSTTSLLEVLNTMYTKDAFFQMMEQLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>R5VHZ3</accession>
    <name>R5VHZ3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Isocitrate dehydrogenase [NADP]</fullName>
            <ecNumber evidence="8">1.1.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BN566_00949</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>D-threo-isocitrate + NADP(+) = 2-oxoglutarate + CO2 + NADPH</text>
            <dbReference type="Rhea" id="RHEA:19629"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8 11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="8 11">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="8 11">Binds 1 Mg(2+) or Mn(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.42" evidence="8"/>
    <dbReference type="EMBL" id="CBAR010000069">
        <property type="protein sequence ID" value="CCZ83166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00949"/>
    <dbReference type="EnsemblBacteria" id="CCZ83166">
        <property type="protein sequence ID" value="CCZ83166"/>
        <property type="gene ID" value="BN566_00949"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004450">
        <property type="term" value="F:isocitrate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004790">
        <property type="entry name" value="Isocitrate_DH_NADP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11822">
        <property type="entry name" value="PTHR11822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000108">
        <property type="entry name" value="IDH_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00127">
        <property type="entry name" value="nadp_idh_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 8 11" id="KW-0464">Manganese</keyword>
    <keyword evidence="3 8 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 8" id="KW-0521">NADP</keyword>
    <keyword evidence="6 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Iso_dh" evidence="12">
        <location>
            <begin position="9"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="10">
        <location>
            <begin position="94"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Critical for catalysis" evidence="9">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <feature type="site" description="Critical for catalysis" evidence="9">
        <location>
            <position position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR000108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CCZ83166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="404" mass="45900" checksum="A233E7E9CAD2AD2E" modified="2013-07-24" version="1">MAKIQMTTPLVEMDGDEMTRILWQWIKDILLTPYVDLKTEYYDLGLDYREKTKDQVTIDSANATLKYGVAVKCATITPNAARMPEYHLSQMWKSPNGTIRAILDGTVFRTPILVKGIEPYIPTWTKPITIARHAYGDVYKNTEMKVKQGSKAELVVTNAEGEETRQLIFDFKDTDGIVQGLHNKNNSIAGFARACFNYGLDLKQDVWFATKDTISKQYDHTFKDIFQEIFDAEYKEKFAEAGIEYFYTLIDDAVARVIRSHGGYIWACKNYDGDVMSDMVSTAYGSLAMMTSVLVSPTGIYEYEAAHGTVQRHYYKYLKGEETSTNSVATIFAWTGALRKRGELDNLPELMQFADKLEAATIQTIEDGVMTGDLYLISKLENKKKVNSKEFLEAIRERLDAAMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>R5VID0</accession>
    <name>R5VID0_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_01102</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000084">
        <property type="protein sequence ID" value="CCZ83321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01102"/>
    <dbReference type="EnsemblBacteria" id="CCZ83321">
        <property type="protein sequence ID" value="CCZ83321"/>
        <property type="gene ID" value="BN566_01102"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015424">
        <property type="term" value="F:ABC-type amino acid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030679">
        <property type="entry name" value="ABC_ATPase_HisP-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039085">
        <property type="entry name" value="ABC_ATPase_HisP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="5"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="37"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="254" mass="28183" checksum="66DAF723D4778248" modified="2013-07-24" version="1">MAEYLTISHLQKQFGDVQVLKDISLTVNQGEVVSIIGPSGSGKSTLLRCATFLETLSSGEIAYMGETAVTAALNAKPVYAPKKQFRKIQTYFGLVFQNFNLFPHYSVLKNIVEPQCILLKRSKKEATENAMKLLKKVGLEEKASAYPCELSGGQQQRVAIVRALAMSPAILFFDEPTSALDPELTGEILAVIRLLAEDHMTMVIVTHEMRFAQAVSDRVIFMADGVIVEEGTPDDLFLHPQKERTKQFLSHYAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJT1</accession>
    <name>R5VJT1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_01536</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="5">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="5">Divalent metal cations. Prefers magnesium or manganese.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the malic enzymes family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000164">
        <property type="protein sequence ID" value="CCZ83801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01536"/>
    <dbReference type="EnsemblBacteria" id="CCZ83801">
        <property type="protein sequence ID" value="CCZ83801"/>
        <property type="gene ID" value="BN566_01536"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004471">
        <property type="term" value="F:malate dehydrogenase (decarboxylating) (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05311">
        <property type="entry name" value="NAD_bind_2_malic_enz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminiacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015884">
        <property type="entry name" value="Malic_enzyme_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012301">
        <property type="entry name" value="Malic_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037062">
        <property type="entry name" value="Malic_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012302">
        <property type="entry name" value="Malic_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045213">
        <property type="entry name" value="Malic_NAD-bd_bact_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001891">
        <property type="entry name" value="Malic_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00390">
        <property type="entry name" value="malic"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03949">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000106">
        <property type="entry name" value="ME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01274">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00919">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="SSF53223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00331">
        <property type="entry name" value="MALIC_ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="malic" evidence="7">
        <location>
            <begin position="15"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Malic_M" evidence="6">
        <location>
            <begin position="160"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="36"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="91"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="392" mass="41616" checksum="8250C42FD3E39AA6" modified="2013-07-24" version="1">MDYAKESLKLHYDWQGKFEIALRAPLETSDDLSLAYTPGVAQPCLEIQKDVDLSYKLTRRGNLVAVVTDGTAVLGLGDIGPEAGMPVMEGKCALFKKFGNVDAIPLCIRSKDVDDIVNTVRLLAGSFGGINLEDISAPRCFEIEEKLKECCDIPIFHDDQHGTAVVTLAAMMNALKLTGKNLKDVKVVTSGAGAAGIAIIRLLIAMGLDPHHVILCDRKGAIYEGREGLNKEKEQMAKITNSGHKAGSLADVLVGADVFIGVSAPNCVTPEMVKSMAEKPILFPMANPTPEIMPEEALAAGAYCVGTGRSDFPNQINNVLAFPGIFRGALDVHAKEINDAMKIAAAKAIASFVTDEKLCPEYIIPSALEKGVAEAVAAAVAQAAKDTGVARI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJW2</accession>
    <name>R5VJW2_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA N6-adenosine threonylcarbamoyltransferase</fullName>
            <ecNumber evidence="6">2.3.1.234</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">N6-L-threonylcarbamoyladenine synthase</fullName>
            <shortName evidence="6">t(6)A synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaD</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">tRNA threonylcarbamoyladenosine biosynthesis protein TsaD</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">tsaD</name>
        <name evidence="8" type="ORF">BN566_01566</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>adenosine(37) in tRNA + L-threonylcarbamoyladenylate = AMP + H(+) + N(6)-L-threonylcarbamoyladenosine(37) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:37059"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.3.1.234"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="6">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the KAE1 / TsaD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.234" evidence="6"/>
    <dbReference type="EMBL" id="CBAR010000165">
        <property type="protein sequence ID" value="CCZ83831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01566"/>
    <dbReference type="EnsemblBacteria" id="CCZ83831">
        <property type="protein sequence ID" value="CCZ83831"/>
        <property type="gene ID" value="BN566_01566"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061711">
        <property type="term" value="F:N(6)-L-threonylcarbamoyladenine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002949">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01445">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000905">
        <property type="entry name" value="Gcp-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017861">
        <property type="entry name" value="KAE1/TsaD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022450">
        <property type="entry name" value="TsaD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735">
        <property type="entry name" value="PTHR11735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735:SF6">
        <property type="entry name" value="PTHR11735:SF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00814">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00789">
        <property type="entry name" value="OSIALOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00329">
        <property type="entry name" value="gcp_kae1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03723">
        <property type="entry name" value="T6A_TsaD_YgjD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="4 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 6" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TsaD" evidence="7">
        <location>
            <begin position="23"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="134"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="338" mass="35378" checksum="E56C0BB8DD3F69D6" modified="2013-07-24" version="1">MLILGIESSCDETAASVAEDYTKIRSNVVASQVKEHQLYGGVVPEIASRRHCENVLPVVQKALDDAQVTLADLDGIAVTYAPGLIGALLVGVNFAKGLALATGKPLVPVHHLAGHIAANYIAHPELKPPYLCLVASGGHSHIVEVLSYTQFRIVGRTRDDAAGECFDKTARAMGFPYPGGIHIDHAAQEGNPEAFHLPRPKVEGNPYDFSFSGLKTAVVNLLHNASQKGQTVPTADLAASVQKTISELLTTHTMQAAKDLGYQTIAVAGGVSANSGVRSAMAAACEKQGCKLFVPPLSLCGDNGAMIACAGSYAYAAGVRADASLNAYATMSLEQPTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5VK59</accession>
    <name>R5VK59_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Short-chain dehydrogenases of various substrate specificities</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01650</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000173">
        <property type="protein sequence ID" value="CCZ83921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01650"/>
    <dbReference type="EnsemblBacteria" id="CCZ83921">
        <property type="protein sequence ID" value="CCZ83921"/>
        <property type="gene ID" value="BN566_01650"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008202">
        <property type="term" value="P:steroid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0753">Steroid metabolism</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="250" mass="27483" checksum="13F6170A27223A12" modified="2013-07-24" version="1">MRAVITGATSGIGKAMACQLAQDGWSLVLTGRNQTVLEQLQKNLPTAVEILPLDLAEEEAPYQLFDFCKKKRVDLLVNNAGFGIFGRFTETNLQEEMDLLAVNVRALHILTKLFLKKFQAQKHGRILNVASSAGFLTGPLLSSYYASKNYVVRLSLAIAEELRRQHSPVTMSILCPGPVDTNFNARAGVSFSVPPMSSQQVAAYALEKTMEGKLLIVPGVGMKLALFGARFVPHTWLSAITYEVQKKKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5VKB3</accession>
    <name>R5VKB3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Inorganic pyrophosphatase</fullName>
            <ecNumber evidence="5">3.6.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Pyrophosphate phospho-hydrolase</fullName>
            <shortName evidence="5">PPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ppa</name>
        <name evidence="6" type="ORF">BN566_01690</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the hydrolysis of inorganic pyrophosphate (PPi) forming two phosphate ions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>diphosphate + H2O = H(+) + 2 phosphate</text>
            <dbReference type="Rhea" id="RHEA:24576"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="EC" id="3.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.1" evidence="5"/>
    <dbReference type="EMBL" id="CBAR010000176">
        <property type="protein sequence ID" value="CCZ83961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01690"/>
    <dbReference type="EnsemblBacteria" id="CCZ83961">
        <property type="protein sequence ID" value="CCZ83961"/>
        <property type="gene ID" value="BN566_01690"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004427">
        <property type="term" value="F:inorganic diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006796">
        <property type="term" value="P:phosphate-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00412">
        <property type="entry name" value="pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00209">
        <property type="entry name" value="Inorganic_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008162">
        <property type="entry name" value="Pyrophosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036649">
        <property type="entry name" value="Pyrophosphatase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10286">
        <property type="entry name" value="PTHR10286"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00719">
        <property type="entry name" value="Pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50324">
        <property type="entry name" value="SSF50324"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="5">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ83961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="176" mass="20409" checksum="EA4BF64FBB2AF8C7" modified="2013-07-24" version="1">MNIWHDINPKQITTEEFIAVIEIPKNSKVKYELNKETGVLEMDRILYTSTHYPANYGFIPRTYADDGDPLDVLVLCSQNIYPMTLVRCYPIGVIRMIDNGRHDDKIIAIPCSDPTYNTYVDIDELPSHIFEEMVHFFKVYKELEHKSTAVDEIQNAKAAKEIIAKDIDNYIETFCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5VL31</accession>
    <name>R5VL31_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Bifunctional riboflavin kinase/FMN adenylyltransferase</fullName>
            <ecNumber evidence="6">2.7.1.26</ecNumber>
            <ecNumber evidence="7">2.7.7.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">BN566_00020</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + FMN + H(+) = diphosphate + FAD</text>
            <dbReference type="Rhea" id="RHEA:17237"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="2.7.7.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + riboflavin = ADP + FMN + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14357"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57986"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; FAD biosynthesis; FAD from FMN: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (ATP route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the RibF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.26" evidence="6"/>
    <dbReference type="EC" id="2.7.7.2" evidence="7"/>
    <dbReference type="EMBL" id="CBAR010000197">
        <property type="protein sequence ID" value="CCZ84251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00020"/>
    <dbReference type="EnsemblBacteria" id="CCZ84251">
        <property type="protein sequence ID" value="CCZ84251"/>
        <property type="gene ID" value="BN566_00020"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00276">
        <property type="reaction ID" value="UER00406"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00277">
        <property type="reaction ID" value="UER00407"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003919">
        <property type="term" value="F:FMN adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008531">
        <property type="term" value="F:riboflavin kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006747">
        <property type="term" value="P:FAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009398">
        <property type="term" value="P:FMN biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015864">
        <property type="entry name" value="FAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023468">
        <property type="entry name" value="Riboflavin_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002606">
        <property type="entry name" value="Riboflavin_kinase_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015865">
        <property type="entry name" value="Riboflavin_kinase_bac/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023465">
        <property type="entry name" value="Riboflavin_kinase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22749">
        <property type="entry name" value="PTHR22749"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06574">
        <property type="entry name" value="FAD_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01687">
        <property type="entry name" value="Flavokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00904">
        <property type="entry name" value="Flavokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82114">
        <property type="entry name" value="SSF82114"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00083">
        <property type="entry name" value="ribF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0274">FAD</keyword>
    <keyword evidence="9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0288">FMN</keyword>
    <keyword evidence="12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Flavokinase" evidence="15">
        <location>
            <begin position="83"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00018483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CCZ84251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="210" mass="23154" checksum="B0500BDE20E55283" modified="2013-07-24" version="1">MDGETFCRVYLHDQMHAKKLFCGRDFRFGNRAAWGVADLAAFGETFGFTVELIDAVEQDGAVISSSRIRQLLLDGKIQQANRLLGAPYQISGIIQHGAALGRTIQFPTINLSLAPEQLVPLYGVYRAKVYLKDGSVWNGVTNIGTKPTVSNACIPVAETHLLGFQGDLYGQFCRIELLDFLRKEQKFSGLDELKAAIADNIRTVQAACKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>R5VL57</accession>
    <name>R5VL57_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Purine nucleoside phosphorylase DeoD-type</fullName>
            <shortName evidence="4">PNP</shortName>
            <ecNumber evidence="4">2.4.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">deoD</name>
        <name evidence="6" type="ORF">BN566_00210</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the reversible phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a purine 2'-deoxy-D-ribonucleoside + phosphate = 2-deoxy-alpha-D-ribose 1-phosphate + a purine nucleobase</text>
            <dbReference type="Rhea" id="RHEA:36431"/>
            <dbReference type="ChEBI" id="CHEBI:26386"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57259"/>
            <dbReference type="ChEBI" id="CHEBI:142361"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a purine D-ribonucleoside + phosphate = a purine nucleobase + alpha-D-ribose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:19805"/>
            <dbReference type="ChEBI" id="CHEBI:26386"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57720"/>
            <dbReference type="ChEBI" id="CHEBI:142355"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homohexamer; trimer of homodimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the PNP/UDP phosphorylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.1" evidence="4"/>
    <dbReference type="EMBL" id="CBAR010000207">
        <property type="protein sequence ID" value="CCZ84281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00210"/>
    <dbReference type="EnsemblBacteria" id="CCZ84281">
        <property type="protein sequence ID" value="CCZ84281"/>
        <property type="gene ID" value="BN566_00210"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004731">
        <property type="term" value="F:purine-nucleoside phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009164">
        <property type="term" value="P:nucleoside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042278">
        <property type="term" value="P:purine nucleoside metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09006">
        <property type="entry name" value="PNP_EcPNPI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01627">
        <property type="entry name" value="Pur_nucleosid_phosp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004402">
        <property type="entry name" value="DeoD-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018016">
        <property type="entry name" value="Nucleoside_phosphorylase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43691:SF2">
        <property type="entry name" value="PTHR43691:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="SSF53167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00107">
        <property type="entry name" value="deoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01232">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PNP_UDP_1" evidence="5">
        <location>
            <begin position="15"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphate binding" evidence="4">
        <location>
            <begin position="88"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Purine nucleoside binding" evidence="4">
        <location>
            <begin position="179"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Purine nucleoside binding" evidence="4">
        <location>
            <begin position="203"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="204"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="5"/>
        </location>
        <ligand>
            <name>a purine D-ribonucleoside</name>
            <dbReference type="ChEBI" id="CHEBI:142355"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="4">
        <location>
            <position position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ84281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="237" mass="25535" checksum="C7F98CBC2ABA2BC0" modified="2013-07-24" version="1">MATPHNQANPGEIAKVVLMPGDPLRAKFIAETYLEHPVCYNTVRNMLGYTGTYQGVPISVQGSGMGMPSMGIYSYELFHFYDVDAIIRIGTAGGVADSVKLRDLVLGMGVCTDSNYAAQYNLPGTFAPIADFSLLEAAVSAARALKKPFHVGNLCSSDHFYTENDPLPKWAKMGVLAVEMESAALYCNAAEAGKKALCITTISDCPLRNESTSAEERQTAFTDMMELALQTAVSVQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5VL75</accession>
    <name>R5VL75_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_00035</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000208">
        <property type="protein sequence ID" value="CCZ84296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00035"/>
    <dbReference type="EnsemblBacteria" id="CCZ84296">
        <property type="protein sequence ID" value="CCZ84296"/>
        <property type="gene ID" value="BN566_00035"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026906">
        <property type="entry name" value="LRR_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13306">
        <property type="entry name" value="LRR_5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004407163" evidence="4">
        <location>
            <begin position="27"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="364"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="327"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="387"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="398"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ84296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="429" mass="46023" checksum="119B6A3564057B3D" modified="2013-07-24" version="1">MKHPFFSLLSASLLLGSLAVGTVVSAIPAAADGTSGDYTYEVDEETLKGYITGYTGKDKEITIPEELDGTVIAGISADTFYNCDTLEKITIPASITEIGESAFYDCDNLKEFAVADDSETYYAKDGVLFRTKDDCLMAYPPAATATSYTVPDGCKELYYAAFGKCNNLEEITLPDSLLYIDDWVFAYDNIDHIAIPDSVVEICDYAFAYCENITEWTLPSSLTYIGNATFAGCTGMTEIVLPNNLTTIGQAAFTGTGLTEITIPSSVEEIGYSAFGYEADMKTAVKNFVIYGQSGSAAQRYASDSDDTYGYKNSFSFVTVQDASKVRGGRDSEDLSDSEEISGTTEAAAATNSESKKSGFNSKYLLLGISGLVLVIGAILVILGLRSGKKTEPESTESDSDSESNSDSDATPSEDDSTEKQDEQTEDAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5VL96</accession>
    <name>R5VL96_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">30S ribosomal protein S20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rpsT</name>
        <name evidence="9" type="ORF">BN566_00229</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Binds directly to 16S ribosomal RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the bacterial ribosomal protein bS20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000212">
        <property type="protein sequence ID" value="CCZ84316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00229"/>
    <dbReference type="EnsemblBacteria" id="CCZ84316">
        <property type="protein sequence ID" value="CCZ84316"/>
        <property type="gene ID" value="BN566_00229"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00500">
        <property type="entry name" value="Ribosomal_S20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002583">
        <property type="entry name" value="Ribosomal_S20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036510">
        <property type="entry name" value="Ribosomal_S20_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33398">
        <property type="entry name" value="PTHR33398"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01649">
        <property type="entry name" value="Ribosomal_S20p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46992">
        <property type="entry name" value="SSF46992"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00029">
        <property type="entry name" value="S20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 7 9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 7" id="KW-0699">rRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ84316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="86" mass="9225" checksum="9A6B82BDAFCD2614" modified="2013-07-24" version="1">MPNIKSAKKRVRVNATKAAANKARKSNLKTMIKKADLACTENAADKEAAIRVAIKKVDQACAKGLLHKNAAARKKSQLAKKLNAAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5VLB0</accession>
    <name>R5VLB0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Thioesterase superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00244</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000215">
        <property type="protein sequence ID" value="CCZ84331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00244"/>
    <dbReference type="EnsemblBacteria" id="CCZ84331">
        <property type="protein sequence ID" value="CCZ84331"/>
        <property type="gene ID" value="BN566_00244"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025540">
        <property type="entry name" value="FlK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36934">
        <property type="entry name" value="PTHR36934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF014972">
        <property type="entry name" value="FlK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="1">
        <location>
            <position position="35"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="43"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="69"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="62"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR014972-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR014972-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="132" mass="13590" checksum="65CE4AD14B4BEC95" modified="2013-07-24" version="1">MQPITIGTKASASVQVTEAQLAVTVGSGSLRVFATPMLAALMEKTACAAVAEFLEDGETTVGTALDLAHTAATPEGMTVTATAEITAVSGREITFAITASDEAGEVGHCTHKRFLVKSESFMKKAAAKKESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5VLS7</accession>
    <name>R5VLS7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 2">Biotin carboxyl carrier protein of acetyl-CoA carboxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00390</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">This protein is a component of the acetyl coenzyme A carboxylase complex; first, biotin carboxylase catalyzes the carboxylation of the carrier protein and then the transcarboxylase transfers the carboxyl group to form malonyl-CoA.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000240">
        <property type="protein sequence ID" value="CCZ84491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00390"/>
    <dbReference type="EnsemblBacteria" id="CCZ84491">
        <property type="protein sequence ID" value="CCZ84491"/>
        <property type="gene ID" value="BN566_00390"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009317">
        <property type="term" value="C:acetyl-CoA carboxylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003989">
        <property type="term" value="F:acetyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001249">
        <property type="entry name" value="AcCoA_biotinCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01071">
        <property type="entry name" value="ACOABIOTINCC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="SSF51230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0092">Biotin</keyword>
    <keyword evidence="2" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="3">
        <location>
            <begin position="15"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00017562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU364072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="91" mass="9614" checksum="ED8B7209A6AA56E2" modified="2013-07-24" version="1">MAAPAPEAKAAAPAGKFVKAPIVGTFYAASAPDQPPFVKVGDQIKKGDVLFIIESMKLMNEVQSEIEGTVAEILVGNGEAVEYDQPILRVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>R5VLY9</accession>
    <name>R5VLY9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein translocase subunit SecY</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">secY</name>
        <name evidence="11" type="ORF">BN566_00077</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the Sec protein translocase complex. Heterotrimer consisting of SecY, SecE and SecG subunits. The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. Interacts with the ribosome. Interacts with SecDF, and other proteins may be involved. Interacts with SecA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9 10">Belongs to the SecY/SEC61-alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000241">
        <property type="protein sequence ID" value="CCZ84531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00077"/>
    <dbReference type="EnsemblBacteria" id="CCZ84531">
        <property type="protein sequence ID" value="CCZ84531"/>
        <property type="gene ID" value="BN566_00077"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043952">
        <property type="term" value="P:protein transport by the Sec complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01465">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026593">
        <property type="entry name" value="SecY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002208">
        <property type="entry name" value="SecY/SEC61-alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030659">
        <property type="entry name" value="SecY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023201">
        <property type="entry name" value="SecY_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10906">
        <property type="entry name" value="PTHR10906"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00344">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004557">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103491">
        <property type="entry name" value="SSF103491"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00967">
        <property type="entry name" value="3a0501s007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00755">
        <property type="entry name" value="SECY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="116"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="186"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="217"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="265"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="320"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="374"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="406"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ84531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="441" mass="49267" checksum="15D9594ED7C80B2A" modified="2013-07-24" version="1">MFKTLRDAWSIKDIRKKLLYTLFMIIIFRLGCSVVVPFLDTSVVSDWMSQKATNGNFLEYLDILTGGALSQATIFSLSITPYINASIIMQLLTYALPPLERLRDEGEEGQKVLNKITAAVALALAVFMSVAYYLTLKNSMNAVKTYDSTAANVFVGIIIVLCFIAGTCIVVWMGNRINDNGIGNGISMLLFAGILARFPTMMGTLVELTKENPQRYFFESLAVLVIFIAMITFIVFMNNSERRIPIQYAKRVVGRKQYGGQSTHIPVKVCMSGVMPIIFAMSFMSLPSTIELFKPMYDDPVGGWQKFYNGFLTFFHTNSWGYAVIYFVLIIAFNYFYVSMQYNPIEIANNLRQSNGGIPGIRPGKPTSDYIQRVLSKITLVGAFFLGIIAIFPIAMNKADPNLSSLAMGGTTILIVVSVALETVRTLESEMMMRHHKGFLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>R5VLZ6</accession>
    <name>R5VLZ6_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5 6">50S ribosomal protein L36</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpmJ</name>
        <name evidence="7" type="ORF">BN566_00082</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the bacterial ribosomal protein bL36 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000241">
        <property type="protein sequence ID" value="CCZ84536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="SMR" id="R5VLZ6"/>
    <dbReference type="STRING" id="1262953.BN566_00082"/>
    <dbReference type="EnsemblBacteria" id="CCZ84536">
        <property type="protein sequence ID" value="CCZ84536"/>
        <property type="gene ID" value="BN566_00082"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00251">
        <property type="entry name" value="Ribosomal_L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000473">
        <property type="entry name" value="Ribosomal_L36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035977">
        <property type="entry name" value="Ribosomal_L36_sp"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42888">
        <property type="entry name" value="PTHR42888"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00444">
        <property type="entry name" value="Ribosomal_L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57840">
        <property type="entry name" value="SSF57840"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01022">
        <property type="entry name" value="rpmJ_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00828">
        <property type="entry name" value="RIBOSOMAL_L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ84536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="37" mass="4319" checksum="7F79BEC2DE1C4CF7" modified="2013-07-24" version="1">MKVRPSVKPICEKCKVIKRKGKVMIICENPKHKQRQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5VM54</accession>
    <name>R5VM54_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">LPXTG-motif cell wall anchor domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_00474</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000257">
        <property type="protein sequence ID" value="CCZ84621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00474"/>
    <dbReference type="EnsemblBacteria" id="CCZ84621">
        <property type="protein sequence ID" value="CCZ84621"/>
        <property type="gene ID" value="BN566_00474"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00222">
        <property type="entry name" value="CollagenBindB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008454">
        <property type="entry name" value="Collagen-bd_Cna-like_B-typ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05738">
        <property type="entry name" value="Cna_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="1" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004395486" evidence="4">
        <location>
            <begin position="33"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="368"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="CNA-B" evidence="5">
        <location>
            <begin position="183"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="326"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ84621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="394" mass="42693" checksum="67DF8C70E3AA63F0" modified="2013-07-24" version="1">MIQRRWFARLRYGAAALLFFLCFWLTPMQTEAATDGMLTIICKDGDTILPNLTWKLYRVGSYSSSGELLLEGDFADYPVNGKLTTTAEVQDAADTLANLTVVDAIAPLSTGKTDQSGAVTFAPLETGAYLITGKPVVVGDKRYIPAPALLYLTEESGSDTFQWNIYPKFTVKPFSTSSTVRYSVEKVWLHDEGMTMNRPEDLVIQLYCDDVMREEVTLNEANGWSYSWEGNSLEEWHVKELVVPKNYLVVYKQNDTQFLIFNSHNQTGSQELTTAPITTTTTTETTTVTTETILSETETTILETTTSVGSSIVSGGTAIVSTTETGTATITSGSGTTSTGSSGSTTSGSGTGTTVTTTKPTLPATGQLWWPVPCLAAGGLVLLGIGWRMDKKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>R5VM59</accession>
    <name>R5VM59_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Guanylate kinase</fullName>
            <ecNumber evidence="2 9">2.7.4.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">GMP kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">gmk</name>
        <name evidence="11" type="ORF">BN566_00479</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Essential for recycling GMP and indirectly, cGMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + GMP = ADP + GDP</text>
            <dbReference type="Rhea" id="RHEA:20780"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the guanylate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.8" evidence="2 9"/>
    <dbReference type="EMBL" id="CBAR010000257">
        <property type="protein sequence ID" value="CCZ84626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00479"/>
    <dbReference type="EnsemblBacteria" id="CCZ84626">
        <property type="protein sequence ID" value="CCZ84626"/>
        <property type="gene ID" value="BN566_00479"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004385">
        <property type="term" value="F:guanylate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00328">
        <property type="entry name" value="Guanylate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017665">
        <property type="entry name" value="Guanylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03263">
        <property type="entry name" value="guanyl_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Guanylate kinase-like" evidence="10">
        <location>
            <begin position="4"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="11"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ84626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="200" mass="22272" checksum="AC41CCE242F9AB42" modified="2013-07-24" version="1">MQKGLLLVVSGPSGCGKGTVLKEVMQQQDLYYSVSLTTRAPRPGEVDGVQYHFVSKEQFEEMIAQDEVLEHAEFCGNYYGTPRDAVEAMRNEGKNVILEIEVQGAQQVMQKCPDAVSIFIAPPSMTELEKRLRGRGTEAEEVIQKRLATASGELKDAPNYKYLVVNDQVETAAQEILTILAAEQIHQINKEEKVKEVLES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>R5VMB2</accession>
    <name>R5VMB2_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Acetylglutamate kinase</fullName>
            <ecNumber evidence="7">2.7.2.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">N-acetyl-L-glutamate 5-phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">NAG kinase</fullName>
            <shortName evidence="7">NAGK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">argB</name>
        <name evidence="9" type="ORF">BN566_00539</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the ATP-dependent phosphorylation of N-acetyl-L-glutamate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamyl 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:14629"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:44337"/>
            <dbReference type="ChEBI" id="CHEBI:57936"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 2/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the acetylglutamate kinase family. ArgB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.8" evidence="7"/>
    <dbReference type="EMBL" id="CBAR010000267">
        <property type="protein sequence ID" value="CCZ84686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00539"/>
    <dbReference type="EnsemblBacteria" id="CCZ84686">
        <property type="protein sequence ID" value="CCZ84686"/>
        <property type="gene ID" value="BN566_00539"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00107"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003991">
        <property type="term" value="F:acetylglutamate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04250">
        <property type="entry name" value="AAK_NAGK-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00082">
        <property type="entry name" value="ArgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004662">
        <property type="entry name" value="AcgluKinase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037528">
        <property type="entry name" value="ArgB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001057">
        <property type="entry name" value="Glu/AcGlu_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041727">
        <property type="entry name" value="NAGK-C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000728">
        <property type="entry name" value="NAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00474">
        <property type="entry name" value="GLU5KINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00761">
        <property type="entry name" value="argB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="1 7" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AA_kinase" evidence="8">
        <location>
            <begin position="26"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="66"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="7">
        <location>
            <position position="31"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="7">
        <location>
            <position position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ84686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="288" mass="31195" checksum="4A059741FA5FECF1" modified="2013-07-24" version="1">MEENYNKLRAQVLVDALPYIQKYNDKIVVVKYGGNAMTNEKLKQAVMSDIVLLSLVGVKVVLVHGGGPEISDMLNRLHIENKFIGGLRYTDKDTVDVVKMVLAGKVNKELVTLLSRNHGKALGLCGIDGDMLRAERKFGANGEDLGYVGDITSVNEQPILDALSNGYIPVIATVASDELGQTYNVNADTAAARIAAQLRAENLILMTDIAGLLRNKDDPSTLIPNVNVSEVPFLKRKGIISGGMIPKIDCCVEAVRRGVKKTAIIDGRVPHSILIETLSSEGIGTQFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5VQF6</accession>
    <name>R5VQF6_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BofC_C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00627</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000011">
        <property type="protein sequence ID" value="CCZ82822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82822">
        <property type="protein sequence ID" value="CCZ82822"/>
        <property type="gene ID" value="BN566_00627"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015050">
        <property type="entry name" value="BofC_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08955">
        <property type="entry name" value="BofC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="BofC_C" evidence="2">
        <location>
            <begin position="55"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ82822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="108" mass="12083" checksum="D21C85FACFEE00D8" modified="2013-07-24" version="1">MTKYRAVSVVLMSILIAAVIVLCALSQSIQNKRVAAKTEESSTLPQVDYVLGEYEGYLAVFRGEQQKPYQVLQMPVQLLPEEDQGAIEAGIHVQTEQELRRLLEDFAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5VQQ7</accession>
    <name>R5VQQ7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Glycoside hydrolase family 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_00704</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000022">
        <property type="protein sequence ID" value="CCZ82902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00704"/>
    <dbReference type="EnsemblBacteria" id="CCZ82902">
        <property type="protein sequence ID" value="CCZ82902"/>
        <property type="gene ID" value="BN566_00704"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002105">
        <property type="entry name" value="Dockerin_1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018087">
        <property type="entry name" value="Glyco_hydro_5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00404">
        <property type="entry name" value="Dockerin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00659">
        <property type="entry name" value="GLYCOSYL_HYDROL_F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="1" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="3 5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004407297" evidence="6">
        <location>
            <begin position="36"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="7">
        <location>
            <begin position="475"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ82902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="537" mass="61144" checksum="C61D45AC65B43A16" modified="2013-07-24" version="1">MKKRTFLQKLCAIFTVSALLLGTGSAIAPAVSAAAEPDSWHDDWLHVNDNAEIVDRNGNPVWLTGCNWFGYNVGSQVFDGVWSQNMHEMLTQIADHGFNLLRIPVSTELLLQWKNGDPDPATPKVNEWTNPELTKEGVVGGTVKYSFDIWNQAIAWCRELGIKIMIDIHSAETASAGHQVNLWYTDKFSTEDWFEALSWFADYYKDDDTILAIDLKNEPHGKADVPDKMAKWDDTTDPNNWKYAAEQAAAHVLKENPNLLVMVEGVEVYPKEGYDWTAPAIDWTTNTEYYYGTWWGGNFRGAKENPIDLGEYQSQLVYSPHDYGPLVYQQKWFYEGFTKETLLKDCWYDNWFYLLDEKVAPLLMGEWGGFIDSQHDPDGSNQKWMLALRDLMIENHIHHTFWCFNENSGDTGGLVYDNFGKWDEDKYALVEPALWQTDSGKFISLDHQTALGANGISLGDYYGSTNPTDPTTPTESPIKGDANGDEMTNILDSMLIRRYLLNPKTILVTENADWNENNTVDLTDLISLNRFLLKQDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>R5VQV4</accession>
    <name>R5VQV4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Anti-sigma F factor</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Stage II sporulation protein AB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">spoIIAB</name>
        <name evidence="5" type="ORF">BN566_00737</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Binds to sigma F and blocks its ability to form an RNA polymerase holoenzyme (E-sigma F). Phosphorylates SpoIIAA on a serine residue. This phosphorylation may enable SpoIIAA to act as an anti-anti-sigma factor that counteracts SpoIIAB and thus releases sigma F from inhibition.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the anti-sigma-factor family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CBAR010000029">
        <property type="protein sequence ID" value="CCZ82937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00737"/>
    <dbReference type="EnsemblBacteria" id="CCZ82937">
        <property type="protein sequence ID" value="CCZ82937"/>
        <property type="gene ID" value="BN566_00737"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016989">
        <property type="term" value="F:sigma factor antagonist activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030436">
        <property type="term" value="P:asexual sporulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042174">
        <property type="term" value="P:negative regulation of sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006468">
        <property type="term" value="P:protein phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030435">
        <property type="term" value="P:sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00637">
        <property type="entry name" value="Anti_sigma_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010194">
        <property type="entry name" value="Anti-sigma_F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13581">
        <property type="entry name" value="HATPase_c_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01925">
        <property type="entry name" value="spIIAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2 3" id="KW-0749">Sporulation</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HATPase_c" evidence="4">
        <location>
            <begin position="37"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ82937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="144" mass="16162" checksum="4360E3694C7455FB" modified="2013-07-24" version="1">MHTTNQMKMVFPGISVNERTARSLVAAFLVQLDPTVEELADIRTAVSEAVTNSIVHGYRSQKGDVELHIKILADREVYIRVKDRGCGIPDIEQAMQPLYTTAKEEERAGLGFAVMQSFMDRLQVRSKPNEGTTVIMRKKLRASL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5VQX2</accession>
    <name>R5VQX2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00752</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000032">
        <property type="protein sequence ID" value="CCZ82952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00752"/>
    <dbReference type="EnsemblBacteria" id="CCZ82952">
        <property type="protein sequence ID" value="CCZ82952"/>
        <property type="gene ID" value="BN566_00752"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024272">
        <property type="entry name" value="MAFF-rel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12750">
        <property type="entry name" value="Maff2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ82952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="67" mass="7238" checksum="52D1BBFCFFB00717" modified="2013-07-24" version="1">MSQPVFLLKSRVLSMLQSGESTYMEFFNQAITVLQTLVVAIGADLGVWGVVNLMEGHGNDNPGAKVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5VQY5</accession>
    <name>R5VQY5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative heme biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_00762</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000032">
        <property type="protein sequence ID" value="CCZ82962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00762"/>
    <dbReference type="EnsemblBacteria" id="CCZ82962">
        <property type="protein sequence ID" value="CCZ82962"/>
        <property type="gene ID" value="BN566_00762"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="5">
        <location>
            <begin position="22"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ82962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="369" mass="42961" checksum="1F720EB4D53008AE" modified="2013-07-24" version="1">MSEPFCFQMSISEFIENNFKEIRAPFFAGIELTAKCNLHCLHCYAKNGIADSDMTTEEVYQIIDKLCESGTLGIYLTGGEALLRPDFFDIYRYAKKKGISVSVLSNITLLNETHIALFTEYPVSEVSTTLYGATEQTYEAVTGVKGSYQKFVKALQLLQENQIPVSLKYVTLRENYHEVYLAQEFAEQHHCRMMTAFSIYSAANGDPFPLQHRVSPQEAFHFDLHDKKRNAFWKQKAQELYLERTGKKEKHIYARKEEGYFYPCDISWHSTFISHLGRMQACTKTSYQSYDLLHGDFQTGWDFLKKEFRDKKKSFACLQCEKFHYCEQCTANFLCDNHSEPQIDPFYCGVAQLRYDYIAHEVKKIEEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>R5VR07</accession>
    <name>R5VR07_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9 10">Chromosomal replication initiator protein DnaA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">dnaA</name>
        <name evidence="14" type="ORF">BN566_00104</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9 11">Belongs to the DnaA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000035">
        <property type="protein sequence ID" value="CCZ82982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00104"/>
    <dbReference type="EnsemblBacteria" id="CCZ82982">
        <property type="protein sequence ID" value="CCZ82982"/>
        <property type="gene ID" value="BN566_00104"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006275">
        <property type="term" value="P:regulation of DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06571">
        <property type="entry name" value="Bac_DnaA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1750.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00377">
        <property type="entry name" value="DnaA_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001957">
        <property type="entry name" value="Chromosome_initiator_DnaA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020591">
        <property type="entry name" value="Chromosome_initiator_DnaA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018312">
        <property type="entry name" value="Chromosome_initiator_DnaA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013317">
        <property type="entry name" value="DnaA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013159">
        <property type="entry name" value="DnaA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038454">
        <property type="entry name" value="DnaA_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010921">
        <property type="entry name" value="Trp_repressor/repl_initiator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30050:SF2">
        <property type="entry name" value="PTHR30050:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00308">
        <property type="entry name" value="Bac_DnaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08299">
        <property type="entry name" value="Bac_DnaA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00051">
        <property type="entry name" value="DNAA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00760">
        <property type="entry name" value="Bac_DnaA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48295">
        <property type="entry name" value="SSF48295"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00362">
        <property type="entry name" value="DnaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01008">
        <property type="entry name" value="DNAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9 10" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="12">
        <location>
            <begin position="147"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="Bac_DnaA_C" evidence="13">
        <location>
            <begin position="359"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="155"/>
            <end position="162"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CCZ82982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="454" mass="51611" checksum="AE49549F3AE26137" modified="2013-07-24" version="1">MNSFDEVFEHVKRYCLEKGQIPEVAVKLWIDALHPVDLSGTDAVFTIQSDFQRSVILSNYEPLLKEAFRNILGFDVNLVIRIEEEFEPEPTQTESTGSEDLALRNQQLEKSFEGANYEYTFSTFIVGRSNEFAYAACTAVAKEPGKSYNPLFIYGPSGLGKTHLMHAIAHEVEQQHPDYNIIYVTSEEFGNDLINGINRNNMASFHDKYRSADVLLIDDIQFFSDKDRMQEEFFHTFNKLHAENKQIVITSDKPPRELKTLEERIRTRFECGLITDITIPDFETRIAIIRRKAELLDLHIPDDVAEFIANRLKTNIRQLEGAVKKLKALKHFAGSPPSISMAQSVIKDILNNDQPVPITVEKIIAEVADVYGVTPEEIRSSKRSAQISTARKVATYVVREITQMPLQSIGTEFGGRDHSTIVYAINNIEAGMKRDANLKQLVEDIIKNIKTKTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>R5VR14</accession>
    <name>R5VR14_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Predicted RNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_00109</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000035">
        <property type="protein sequence ID" value="CCZ82987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00109"/>
    <dbReference type="EnsemblBacteria" id="CCZ82987">
        <property type="protein sequence ID" value="CCZ82987"/>
        <property type="gene ID" value="BN566_00109"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02414">
        <property type="entry name" value="jag_KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02644">
        <property type="entry name" value="R3H_jag"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038008">
        <property type="entry name" value="Jag_KH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038247">
        <property type="entry name" value="Jag_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039247">
        <property type="entry name" value="KhpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032782">
        <property type="entry name" value="KhpB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001374">
        <property type="entry name" value="R3H_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036867">
        <property type="entry name" value="R3H_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034079">
        <property type="entry name" value="R3H_KhpB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35800">
        <property type="entry name" value="PTHR35800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14804">
        <property type="entry name" value="Jag_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01424">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01245">
        <property type="entry name" value="Jag_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00393">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82708">
        <property type="entry name" value="SSF82708"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51061">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0133">Cell shape</keyword>
    <keyword evidence="5" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="R3H" evidence="7">
        <location>
            <begin position="224"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="114"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="292"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="295"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="331"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="354"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ82987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="378" mass="41932" checksum="F03EF213E30E1E57" modified="2013-07-24" version="1">MRQIFTAKKLEDAKRQAVQAFGVSESQIVFRILEQKKGLMGIGASVTIEAIYEPPVAAVETPAPAAEPMPEPQAAAPVVEETVKTITETVVEPETDAVVEETVSVTETVTVTEPAADAESEAVATESEEPLSLDSLTSEQQAAVKRVQDYVAAVYEKLGVTVTTTPKVQENGILLEISSNSRGGTIIGRRGETLDAMQYLSSIIANKGEEDYCRIILDSNGYREKRRQTLEHLAERLAKTVIRNQRATTLEPMNPYERRIIHAKVAEIDGVSSKSVGEDPYRKVVILPLDENGEPIPARRSRGRNNRNDNRNDRKGGYRNHGNGGNRKRNGNPHRNREEEIPHKPTNFDDIMSTSFEKDYRKPKPEDDLQVGLYGKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5VR96</accession>
    <name>R5VR96_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">5'-deoxynucleotidase</fullName>
            <ecNumber evidence="5">3.1.3.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_00860</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxyribonucleoside 5'-phosphate + H2O = a 2'-deoxyribonucleoside + phosphate</text>
            <dbReference type="Rhea" id="RHEA:36167"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18274"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:65317"/>
            <dbReference type="EC" id="3.1.3.89"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.89" evidence="5"/>
    <dbReference type="EMBL" id="CBAR010000051">
        <property type="protein sequence ID" value="CCZ83072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00860"/>
    <dbReference type="EnsemblBacteria" id="CCZ83072">
        <property type="protein sequence ID" value="CCZ83072"/>
        <property type="gene ID" value="BN566_00860"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002953">
        <property type="term" value="F:5'-deoxynucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039356">
        <property type="entry name" value="YfbR/HDDC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11845">
        <property type="entry name" value="PTHR11845"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13023">
        <property type="entry name" value="HD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HDc" evidence="6">
        <location>
            <begin position="32"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ83072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="201" mass="23246" checksum="5AF8F146C0BEFFA4" modified="2013-07-24" version="1">MTDRLKQQLDFILEIDQEKNILRQTHLSHHGRRENDAEHAWHMTIMAYLLKEYANEPIDIAKVMMMCLLHDVVEIDAGDTYAYDEVGKQTQQAREAAAKERIYSLLPDDQKQELQALFDEFEARQTPESKFAHAMDNLQPLLLNDSNQGSDWREHTVTAKQVYHRQIQTKDGSEVLFDLTDQILQKNIADGNLPDTAPKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>R5VRR3</accession>
    <name>R5VRR3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 9">Homoserine dehydrogenase</fullName>
            <ecNumber evidence="5 9">1.1.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BN566_01028</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>L-homoserine + NAD(+) = H(+) + L-aspartate 4-semialdehyde + NADH</text>
            <dbReference type="Rhea" id="RHEA:15757"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="1.1.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>L-homoserine + NADP(+) = H(+) + L-aspartate 4-semialdehyde + NADPH</text>
            <dbReference type="Rhea" id="RHEA:15761"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="1.1.1.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4 9">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 3/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 9">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the homoserine dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.3" evidence="5 9"/>
    <dbReference type="EMBL" id="CBAR010000081">
        <property type="protein sequence ID" value="CCZ83247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01028"/>
    <dbReference type="EnsemblBacteria" id="CCZ83247">
        <property type="protein sequence ID" value="CCZ83247"/>
        <property type="gene ID" value="BN566_01028"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00063"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00465"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004412">
        <property type="term" value="F:homoserine dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005106">
        <property type="entry name" value="Asp/hSer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001342">
        <property type="entry name" value="HDH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019811">
        <property type="entry name" value="HDH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00742">
        <property type="entry name" value="Homoserine_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03447">
        <property type="entry name" value="NAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01042">
        <property type="entry name" value="HOMOSER_DHGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0412">Isoleucine biosynthesis</keyword>
    <keyword evidence="9" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="7 9" id="KW-0521">NADP</keyword>
    <keyword evidence="8 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="NAD_binding_3" evidence="12">
        <location>
            <begin position="11"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Homoserine_dh" evidence="11">
        <location>
            <begin position="135"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CCZ83247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="417" mass="45075" checksum="F25A60609617212B" modified="2013-07-24" version="1">MQKKKQIAVLGYGVVGSGVVELFYQNQEKIEKNTGYSMDIKYILDLRTFEGDPYADKVVHDINVILQDPEVGYVAECMGGVNPAFEFVSSCLKAGKSVSTSNKQLVAEKGDILLQTAKENNCNFFFEASVGGAIPIMRPLHTCLAANDLYAVAGILNGTTNFILEKMFCDQMSFADALALAQQLGYAERNPSADVDGADACRKICIISSLVFGKHVYPDSVFTKGIRDIQLEDVKLAQQLGGTVKLIAQVVREDDGKILPTVMPMVVMQDSLLSHVNGVFNAIMVWGDGIDKTMFYGRGAGKMPTASAVLGDIIDEVKSNGTVPAQTWETSSDTDFIANIDTFVAPRMFRTSTCAADVAKQAAGQVGVTVRMHTENGFLVSACTERIAAKLTDALQGLGVTVYSQIPVLKDHSKFSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5VS64</accession>
    <name>R5VS64_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01158</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000093">
        <property type="protein sequence ID" value="CCZ83382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83382">
        <property type="protein sequence ID" value="CCZ83382"/>
        <property type="gene ID" value="BN566_01158"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="170"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="193"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="233" mass="26018" checksum="E68398C8F59C2C01" modified="2013-07-24" version="1">MKKQQLSAEQKRLETDLWIIALVTLGVFLFYAATGKQLMSFVANSNISVIPRLLLNAGVQFGVAGLGITIVCILRKESFTHFGLIRKNIFKAIIGTIICFVPSICYIFLSGQFNGYQPFSILITDDVIASGIPFSILGMALIVLVWGFFEGFNYVVICDKINRRYPTTNEWLDYGAITCAIVCILFHPFSTSFWGIVEIITTVIAIYGMLMVKKKTGNAWGCVFAFCFIWNAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>R5VS81</accession>
    <name>R5VS81_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dephospho-CoA kinase</fullName>
            <ecNumber evidence="1">2.7.1.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Dephosphocoenzyme A kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">coaE</name>
        <name evidence="2" type="ORF">BN566_01173</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the phosphorylation of the 3'-hydroxyl group of dephosphocoenzyme A to form coenzyme A.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3'-dephospho-CoA + ATP = ADP + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18245"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57328"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.24"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 5/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CoaE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.24" evidence="1"/>
    <dbReference type="EMBL" id="CBAR010000094">
        <property type="protein sequence ID" value="CCZ83397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01173"/>
    <dbReference type="EnsemblBacteria" id="CCZ83397">
        <property type="protein sequence ID" value="CCZ83397"/>
        <property type="gene ID" value="BN566_01173"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00241">
        <property type="reaction ID" value="UER00356"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004140">
        <property type="term" value="F:dephospho-CoA kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00376">
        <property type="entry name" value="Dephospho_CoA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001977">
        <property type="entry name" value="Depp_CoAkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01121">
        <property type="entry name" value="CoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00152">
        <property type="entry name" value="TIGR00152"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51219">
        <property type="entry name" value="DPCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 2" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="17"/>
            <end position="22"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="212" mass="24036" checksum="3A362A9316CB62C4" modified="2013-07-24" version="1">MNSLNGVMVVGLTGQTGAGKSTVSRLFAESGFCVIDADQIARAVVHKGSRCLLELTECFSDILLLNGELNRKALAKQAFQNKKNLELMNSIMYPYIMGEILRQIHHHSRMGQKLILLDAPTLFESRADDFCELIISVTARAELRKARILQRDSITEEAAQARMDAQLPEQFFVEHSDFIIKNNKDCRNLEVVSEEVIAKIKEYYIRRYQVPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5VSL8</accession>
    <name>R5VSL8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sensor histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01276</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000119">
        <property type="protein sequence ID" value="CCZ83507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01276"/>
    <dbReference type="EnsemblBacteria" id="CCZ83507">
        <property type="protein sequence ID" value="CCZ83507"/>
        <property type="gene ID" value="BN566_01276"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032834">
        <property type="entry name" value="NatK_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14501">
        <property type="entry name" value="HATPase_c_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="183"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="HATPase_c_5" evidence="2">
        <location>
            <begin position="325"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="429" mass="48757" checksum="26C0306C4CCC35D8" modified="2013-07-24" version="1">MMMEFLANAIVGISAAFSGMWVLFQILERRSHFLWRLLGVLGIAIVQGALGLLQIPILNMTSTVLMIVGSTLLCYQCKKLYFLVYDILIVFCGFIADVFSVLLLSTYYNSSISSTLQENNFLVRSIFACLMTFLLCQLLFTLIRRKHAIFSWYEAIFYTMLATGEIAVFAYVCKYIQAYSSGMFLILFLIGCLVLDGYIVVVFYRISIVRKTEKENALLQQQSEMQLEVYSDLQQRYQNSMRTIHDAKRHVRALESLIQSDHLAEAQQYKESLFQKLNELQPSIQCDNPLLSAILNHVFLKAEQRHIALKMDLQGEEQLSMLAGVDLTTIVSNLLDNAVDAVLELPEEQRYIHFAVAFQMGEIMIHVENPTKNDLKREGNTVVSTKEGHFGLGLKNVEMVVKKYKGDFKTDVEDGIFTAAITIPTATNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>R5VST8</accession>
    <name>R5VST8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">UDP-N-acetylenolpyruvoylglucosamine reductase</fullName>
            <ecNumber evidence="16">1.3.1.98</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">UDP-N-acetylmuramate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">murB</name>
        <name evidence="18" type="ORF">BN566_01360</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 16">Cell wall formation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 16">
            <text>NADP(+) + UDP-N-acetyl-alpha-D-muramate = H(+) + NADPH + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine</text>
            <dbReference type="Rhea" id="RHEA:12248"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:68483"/>
            <dbReference type="ChEBI" id="CHEBI:70757"/>
            <dbReference type="EC" id="1.3.1.98"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 16">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5 16">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 16">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the MurB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.98" evidence="16"/>
    <dbReference type="EMBL" id="CBAR010000144">
        <property type="protein sequence ID" value="CCZ83597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01360"/>
    <dbReference type="EnsemblBacteria" id="CCZ83597">
        <property type="protein sequence ID" value="CCZ83597"/>
        <property type="gene ID" value="BN566_01360"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008762">
        <property type="term" value="F:UDP-N-acetylmuramate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.78.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00037">
        <property type="entry name" value="MurB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003170">
        <property type="entry name" value="MurB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011601">
        <property type="entry name" value="MurB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036635">
        <property type="entry name" value="MurB_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21071">
        <property type="entry name" value="PTHR21071"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02873">
        <property type="entry name" value="MurB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="SSF56176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56194">
        <property type="entry name" value="SSF56194"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00179">
        <property type="entry name" value="murB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 16" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7 16" id="KW-0132">Cell division</keyword>
    <keyword evidence="11 16" id="KW-0133">Cell shape</keyword>
    <keyword evidence="15 16" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6 16" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 16" id="KW-0274">FAD</keyword>
    <keyword evidence="8 16" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10 16" id="KW-0521">NADP</keyword>
    <keyword evidence="13 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12 16" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="17">
        <location>
            <begin position="32"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="active site" evidence="16">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="16">
        <location>
            <position position="227"/>
        </location>
    </feature>
    <feature type="active site" evidence="16">
        <location>
            <position position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CCZ83597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="302" mass="32383" checksum="DDB2A86129C3AC43" modified="2013-07-24" version="1">MQGLESITACCNAHACPILPAELLSKHTTFRIGGACQAMVQIANREGLQAILQCCQEQHLPWVVLGNGSNILAADEGYHGIVLQLGRAFSNVRQLDETTLFCEAGATLSAVGKVALEACLTGMECLAGIPGTIGGAVYMNAGAYGGEMKDVVRSVEYLDETGTFRTATPEELQFSYRHSMFSGTKKIVTGVTLQLQAGKYADIREEMQKLKNQRVAKQPLELPSAGSTFKRPVGSYASFLIDQCGLKGASVGDAQVSVKHAGFVVNTGHATCRDVLELCDFVIETVKEKTGYCLELEPVILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5VSW5</accession>
    <name>R5VSW5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00154</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000146">
        <property type="protein sequence ID" value="CCZ83627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00154"/>
    <dbReference type="EnsemblBacteria" id="CCZ83627">
        <property type="protein sequence ID" value="CCZ83627"/>
        <property type="gene ID" value="BN566_00154"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="447" mass="50551" checksum="0653A6C18CB9A405" modified="2013-07-24" version="1">MDADYFKKLQAVPPFAAAATALQTGDLFSDLTGAVTAPVLYSYVWWCLQQAIHDQVDTLYFLARDGHILYEIAKRFCEQYALPIHCQYLYVSRMALRMPAYHRMGEAAYDLLLVRGANLTPRHVLERLALSAEEQQQVYADCAIPAEDCDKSLSVQAFTALAEQLRKSSVYRNFVQEKSQAAETAAMAYLEQAGLLRDVRIGIVDSGWNGSMQNCLMTLLEKGAGKPLPNLKGYYFGLYTEPKQGTWEAWYFDPLTPLKKVICFNNNVYECMCAAPHGTTMGYTIQEDGKAVPICKPMGAVDQANAALAEQQERICLAFTERVLPQVSFSDFAALPLADVTESLLQRLLFRPTAEEAQAFGQFQFCDDMGEMYAFPLASAGQEVALKQGLLSQHVLQHFHHVESVQAEPFWVYGSLACSQIRRKRWYHWNFACWDWIRHAVNRRRGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5VTB9</accession>
    <name>R5VTB9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GRAM_POS_ANCHORING domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_01522</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000162">
        <property type="protein sequence ID" value="CCZ83787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83787">
        <property type="protein sequence ID" value="CCZ83787"/>
        <property type="gene ID" value="BN566_01522"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019931">
        <property type="entry name" value="LPXTG_anchor"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50847">
        <property type="entry name" value="GRAM_POS_ANCHORING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0134">Cell wall</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0572">Peptidoglycan-anchor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0964">Secreted</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004407309" description="GRAM_POS_ANCHORING domain-containing protein" evidence="6">
        <location>
            <begin position="29"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="518"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="GRAM_POS_ANCHORING" evidence="7">
        <location>
            <begin position="510"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="484"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="33"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="543" mass="57960" checksum="B467AD21675EC4CC" modified="2013-07-24" version="1">MKMKKMLAILAALSVLGTTGLTVGAEDAATTEPATEAITTTEPTEETTEAETTAVEAETTEETVAAEENQVVGMEDASADISISGVAFTVKVSVAADNSVVLSVLKGGVETNLSNICVESRVTGELQVNDYYSVNDLNGLVIHAVDGNHIYTWDEAYNGFTACSLNEDGEEKDTKINDASTFQMNGKTVKVVLFGYANDKDELLRLSVYVDGKLTNLSGIVVNSNLQGIQGSVNYTDEIVYQYSARQYFSVSGNVLTIKPRSGYGTTASYTLDAATNTFVASTAPTTTATTTDSTTTTTTTIDPAETEEALRKIFEEYVDEKKKELPYTTAIDSFYYDESAKKAYGIYTCEDSAYLSVLCIITKDGVVEQSNITYKKDAHRFEEAVCFTCNGSTFIAAIVNLSSNGGNLYALNIYNVSGNEPTRTNLYTIYVGNVISPYGYEITDPDSSIALDDYFTVKSDSVITVKGVDYTYDTESGQFVKVGSDSGKTDNGSASNAPASTATKNTSGTPKTGDVTTVPAVAVGLTLTAAGVVAFISKRKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>R5VU57</accession>
    <name>R5VU57_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Site-specific recombinase XerD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_01768</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000181">
        <property type="protein sequence ID" value="CCZ84042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01768"/>
    <dbReference type="EnsemblBacteria" id="CCZ84042">
        <property type="protein sequence ID" value="CCZ84042"/>
        <property type="gene ID" value="BN566_01768"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044068">
        <property type="entry name" value="CB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51900">
        <property type="entry name" value="CB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core-binding (CB)" evidence="5">
        <location>
            <begin position="6"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="Tyr recombinase" evidence="4">
        <location>
            <begin position="136"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ84042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="357" mass="41241" checksum="4508DA353141EBD2" modified="2013-07-24" version="1">MPIDRTACPEFLNDYILYLSMSKSLSPRTVEEYYLDIRLFLQYILMMKDKQYVKLDETDMQKISIVDFPVSLLDTIKLQDLYEFLYYVTESRDNHDRARGRKVSAIKSLFHYLYVNKELIHQNPTEYLELPSLKMSLPKYLTLEQSKSLLEHMDTAYPERDYCIITLFLNCGLRLSELVGLNLNHINLKETKMRVFGKGRKERMVYLNHACMNALDDYLEARKKIKNSQEEPALFLSKRGSRISKRRVQQIVEDALQRAGLSGQGYSTHKLRHTAATLLYQYGNVDTLTLKEILGHKSTATTEIYTHLSNAILQEAADHSPLAEIINHPASDESEPDAPDVVSVVLPDVLPENENKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5VUF4</accession>
    <name>R5VUF4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Tyrosine lyase ThiH</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01877</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000193">
        <property type="protein sequence ID" value="CCZ84152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01877"/>
    <dbReference type="EnsemblBacteria" id="CCZ84152">
        <property type="protein sequence ID" value="CCZ84152"/>
        <property type="gene ID" value="BN566_01877"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010722">
        <property type="entry name" value="BATS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012726">
        <property type="entry name" value="ThiH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034428">
        <property type="entry name" value="ThiH/NoCL/HydG-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43583">
        <property type="entry name" value="PTHR43583"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43583:SF1">
        <property type="entry name" value="PTHR43583:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06968">
        <property type="entry name" value="BATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00301">
        <property type="entry name" value="2-iminoacetate_synthase_(ThiH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00876">
        <property type="entry name" value="BATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02351">
        <property type="entry name" value="thiH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BATS" evidence="2">
        <location>
            <begin position="271"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="387" mass="44150" checksum="6C120C009D52F8A3" modified="2013-07-24" version="1">MRTIYDPMTYQPHMQQLDSQMFEEVQAVMQAFEQTTYTAKEVQAALRSTACTVQEFAALLSPAAQPFLEQMAEKAKREREKHFGNCVQLFTPLYISNYCENECVYCGFNCKNKIHRSHLNMEEVEKEMQAIAKTDMREVLLLTGESRSASSVEYIGNAVQIAKKYFPTIGLEVYPMNVDEYQYLNQCGADFVTVFQETYHLDCYEKMHQAGAKRCMPYRFYAQERAILGGMRGVGFGALLGLSNFRSDMFAAGYHAYLLQKKYPHAEIAMSFPRLRPYKNHEKTEENDVHETQLLQMMLAVRIFLPFASITISTRERAGFRDHVLPISATKISAGVNVGIGGHTEEEQKGDAQFEISDGRSLAEIHQAILEQGMQPVYTDYINTNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5VUG1</accession>
    <name>R5VUG1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01882</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000193">
        <property type="protein sequence ID" value="CCZ84157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84157">
        <property type="protein sequence ID" value="CCZ84157"/>
        <property type="gene ID" value="BN566_01882"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="85" mass="9657" checksum="D62C1EDB48D6B2E3" modified="2013-07-24" version="1">MDYKPYKPFHDNGKDFAKLAVTCQVLQDMEKEEHKQGQTTISSKDVKPRTKEQEKADNIYCICCASIVGLSILVTIIALIVQHFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5VUU1</accession>
    <name>R5VUU1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00031</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000208">
        <property type="protein sequence ID" value="CCZ84292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84292">
        <property type="protein sequence ID" value="CCZ84292"/>
        <property type="gene ID" value="BN566_00031"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="330" mass="37759" checksum="458506211891B37D" modified="2013-07-24" version="1">MNGITLALTRFWQNKQISLPPMSTLLIAAGIMFFCFWVVTSQKQKEQEERAKARKNVFQNLDAAIAAAPICEHIPCRTNPAMFYFLGVQTGIISEQEDAGILCSYLTHWMQTGAVTWQRLSGRSFSLHFEELQAGATLCEQALWNALCALVGEKRTATGKQLLQWSKCWEHSSFLRNGRTGSRSLYEWYLELNDLVSAELEQNGSIQIERVETSQRLFHKKQVKISLSSVLQEEVVQIAGWKQFLKQKQPLQHGRDWPVSSWEACLLFGTILGLGDEVESQLQQCCTAEQREQFSRLPVDHTLFLIRDCCYQMLANCRRAKELVEQRSTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>R5VUV9</accession>
    <name>R5VUV9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Ribonuclease H</fullName>
            <shortName evidence="5">RNase H</shortName>
            <ecNumber evidence="4 5">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rnhA</name>
        <name evidence="7" type="ORF">BN566_00220</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="5">Binds 1 Mg(2+) ion per subunit. May bind a second metal ion at a regulatory site, or after substrate binding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 5">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the RNase H family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="4 5"/>
    <dbReference type="EMBL" id="CBAR010000211">
        <property type="protein sequence ID" value="CCZ84307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00220"/>
    <dbReference type="EnsemblBacteria" id="CCZ84307">
        <property type="protein sequence ID" value="CCZ84307"/>
        <property type="gene ID" value="BN566_00220"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09278">
        <property type="entry name" value="RNase_HI_prokaryote_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00042">
        <property type="entry name" value="RNase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022892">
        <property type="entry name" value="RNaseHI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00075">
        <property type="entry name" value="RNase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50879">
        <property type="entry name" value="RNASE_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase H type-1" evidence="6">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="9"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ84307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="146" mass="16220" checksum="116D840A9EDDC136" modified="2013-07-24" version="1">MKTVEIFTDGACSGNPGAGGWGAVLRYGAKEKELSGGAAQTTNNRMELTGVIMALSALKEPCHVVLTTDSKYVVDSVTKGWVYGWQKRGWVKADKKPALNVDLWQQLLPLLERHEVEFVWVKGHAGHPENERCDRMAVEQRDFYSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5VUZ5</accession>
    <name>R5VUZ5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Alpha-glucosidases family 31 of glycosyl hydrolases</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00255</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the glycosyl hydrolase 31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000217">
        <property type="protein sequence ID" value="CCZ84342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00255"/>
    <dbReference type="EnsemblBacteria" id="CCZ84342">
        <property type="protein sequence ID" value="CCZ84342"/>
        <property type="gene ID" value="BN566_00255"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000322">
        <property type="entry name" value="Glyco_hydro_31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030458">
        <property type="entry name" value="Glyco_hydro_31_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025887">
        <property type="entry name" value="Glyco_hydro_31_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13802">
        <property type="entry name" value="Gal_mutarotas_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01055">
        <property type="entry name" value="Glyco_hydro_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="SSF74650"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00129">
        <property type="entry name" value="GLYCOSYL_HYDROL_F31_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gal_mutarotas_2" evidence="3">
        <location>
            <begin position="46"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="660" mass="75385" checksum="B994F00246BCDCF2" modified="2013-07-24" version="1">MIKKYVYGTPFPTDAVVLDVQPSTDALPFFETDQQGTFTYSLSENDIVYGLGEQIRGINKRGWQYVSWNYDNPNHHEDARSLYGAHNFIIIHGAVTFGAFFDYAGKMEFDIGYTKRSVMQIHAASNNLTVYIITGESEKDIVKQFRKMVGRSYIPPFWAFGYGQSRWGYKTEEDIRTVAKQYQDAGIPLDSIYLDIDYMERYKDFTVDTERFPNLKGLATDMKEQGIHLVPIIDAGVKIEDGYDVYEEGVQNNYFCKNAEGGDFVGAVWPGRVHFPDFLQPKARDWFGKKYAVLTEQGIDGFWNDMNEPAIFYTEDRLADTCAEIEKLTAGNMGIQEYFAFTGMVAGLNGNIGDYDKFYHNVDGKMVKHSEVHNLYGMNMTRSAFEALQEISPEKRTLFFSRSSYIGAHRYGGIWQGDNKSWWSHILQSMQQLPALNMAGFLFTGSDTGGFGCDTTEDLMIRWLQYSLFTPLFRNHSADGTREQELYQFSNVEAAGKMIQIRYSLIPYLYSEFLKAALNDEMMFKPLAFDFPDDAMAEQVEDQLLLGNELMIAPIYKQNAQGRYVYLPEEMMLVRMHSSKDYTTEILPKGHHYVSVGLDELVFFIRNQKAIPFAESAENTAKTDYNTIQLLGYEGSSYELYADNGTSPNPEDSLTIKTLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5VV22</accession>
    <name>R5VV22_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">MIP family channel protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_00274</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MIP/aquaporin (TC 1.A.8) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000220">
        <property type="protein sequence ID" value="CCZ84367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00274"/>
    <dbReference type="EnsemblBacteria" id="CCZ84367">
        <property type="protein sequence ID" value="CCZ84367"/>
        <property type="gene ID" value="BN566_00274"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015267">
        <property type="term" value="F:channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022357">
        <property type="entry name" value="MIP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19139">
        <property type="entry name" value="PTHR19139"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="SSF81338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00221">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="48"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="94"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="172"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ84367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="205" mass="20724" checksum="08D196785678960B" modified="2013-07-24" version="1">METGFLGVVAIALAFGLAIVASAYAIGDISGCHVNPAVSLAMLINGKLTFGDFIGYVIAQVAGAFAGSGILKLITANSDLSGAGVNGYGSLSGVGLHAGAAFVVEVILTFVFIMTILGVTRTKKTASIAGLVIGLTLTFVHLIGIPLTGTSVNPARSLAPAIFAGGDALKQVWLFILAPFVGAILAGLIHKAFYANVADEEESES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-09-29" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5VV75</accession>
    <name>R5VV75_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00319</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000225">
        <property type="protein sequence ID" value="CCZ84412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84412">
        <property type="protein sequence ID" value="CCZ84412"/>
        <property type="gene ID" value="BN566_00319"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004407355" evidence="1">
        <location>
            <begin position="29"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="256" mass="27602" checksum="4919EC9341488D7F" modified="2013-07-24" version="1">MYPARIFAVLLTICLIICSLSLPVSATATVLPYTQSSDSLTTNGLYLRLNLRNEWGNSNCTDLQPSSASDYLSVTFTISGLGKLSEPYVVSLLGAAEDLTFFGTEEDTATPGEVHITRDGTYQVKTLLPHAVAEVFCLMLETNIPLAAYTDTMVAADTGIAITVQQITTEADAPVVQSERGDPDGDGTVSVQDAVMVLTYYAKKSVGLKPETDAQFQYIQRGDIDEDGVISVQDAVLILTYYAKKSAGYAPDWDNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVN7</accession>
    <name>R5VVN7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Exopolysaccharide biosynthesis polyprenyl glycosylphosphotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN566_00428</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial sugar transferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000246">
        <property type="protein sequence ID" value="CCZ84567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00428"/>
    <dbReference type="EnsemblBacteria" id="CCZ84567">
        <property type="protein sequence ID" value="CCZ84567"/>
        <property type="gene ID" value="BN566_00428"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003362">
        <property type="entry name" value="Bact_transf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017475">
        <property type="entry name" value="EPS_sugar_tfrase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02397">
        <property type="entry name" value="Bac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03025">
        <property type="entry name" value="EPS_sugtrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="93"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="264"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Bac_transf" evidence="9">
        <location>
            <begin position="262"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="448"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ84567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="479" mass="55308" checksum="B4DD1F3CA3B6E7A0" modified="2013-07-24" version="1">MCQKKKAFVENRDQYRGVFSFCSIGVLLAALTGCFAFIWYRFYSDFLLLPFYRRGNWVMILLYAVLVLLFGKAFGGLKVGYLKRSDSFYSQMLSMFCVNLVTYLQVSLISRHFARLLPFVILMGIDLIILLLWIFFCNKLYFQLYPPRRLVVVYGDRAAAELVLKMSQRVEKYMICACVDSHQEKSAIVETIANYEGVILCDVPGQLRNDLLKYCFSRQMRTYIVPKISDIIIRGGEEIRLFDTPLVLCRNSGLRLEQRFVKRLFDLAFSLILAVPVLLVSIGVCIAIKLDDGGPVLYRQKRLTYNGKPFYVYKFRSMVCNAEEHGPQLAKSEDDRITRVGRVLRKCRLDELPQLWNIFKGDMAVVGPRPERPELAEKYEEEMPEFAFRLRVKAGLTGYAQVIGLYDTTPYDKLKMDLMYIEKYSLLLDLQILLMTIKTALFPGESNEELHEQTHIPVQKHVAEPEAAASEEKKENKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVP2</accession>
    <name>R5VVP2_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">bPH_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00438</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000247">
        <property type="protein sequence ID" value="CCZ84577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00438"/>
    <dbReference type="EnsemblBacteria" id="CCZ84577">
        <property type="protein sequence ID" value="CCZ84577"/>
        <property type="gene ID" value="BN566_00438"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005182">
        <property type="entry name" value="DUF304"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03703">
        <property type="entry name" value="bPH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="bPH_2" evidence="1">
        <location>
            <begin position="26"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="150" mass="17197" checksum="F4AD8CEAEC643074" modified="2013-07-24" version="1">MARQDDRGTILWTDKKRTFLGLPLSFTRYYLTDHQLITRVGFLSIKEDEIELYRIVDKRIEQTFFQRLFGCGTVIILSRDEDTPEKHLVSIRHPQQVSKLIGNCVRERQENLRIAGRDMIGAAFVGDGNGRISAPYQAPGDLDGEDDDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5UX97</accession>
    <name>R5UX97_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Mate efflux family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00728</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000029">
        <property type="protein sequence ID" value="CCZ82928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82928">
        <property type="protein sequence ID" value="CCZ82928"/>
        <property type="gene ID" value="BN566_00728"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006603">
        <property type="entry name" value="DinF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="234"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="278"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="317"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="362"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="385"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="419"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="452" mass="48963" checksum="1005208B803C1558" modified="2013-07-24" version="1">MTKELTHGKPLPLILQFAFPLILGYMLQQLYSLCDTVIVGRCLGVNELAAVGSTGAVSFLVVGFATGMCTGMSIPVAQAFGAGDYQKLRKYLANSFYLSALISIVMTVGTIIFTGDLLRLMKTPEELYDFAYDYIFTVFAGISATILYNLLSSMLRSIGDSRTPLIFLAIAAALNIGLDFLFILGFHTGVAGAGYASVLSQGISGILCLIYMFKKYEIFRIRKEEWKVSLSCCGRLVSIGLPMALQFSITAIGSIILQTAVNTLGTVSVTAVTAASKLQMILTGPMECLGITMATYCGQNRGAREFERIRSGIRTSVVVSMIYCVVSAVLIIVLGGWMALLFLDASETEVMAQVKEYLRLNAYFYPTLGLLFILRNGLQGMSYSFLPMMAGVTELIARVLVCVAFVPDFGYTAACMASPVAWIFADTLLVTVYFVKMKSLKVQLAQPAPAAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-09-29" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXH2</accession>
    <name>R5UXH2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Dockerin type I repeat</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00786</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000036">
        <property type="protein sequence ID" value="CCZ82993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82993">
        <property type="protein sequence ID" value="CCZ82993"/>
        <property type="gene ID" value="BN566_00786"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002105">
        <property type="entry name" value="Dockerin_1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026906">
        <property type="entry name" value="LRR_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13306">
        <property type="entry name" value="LRR_5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00448">
        <property type="entry name" value="CLOS_CELLULOSOME_RPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004406530" evidence="2">
        <location>
            <begin position="26"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="3">
        <location>
            <begin position="415"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ82993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="488" mass="53608" checksum="AEAD57C80B40098E" modified="2013-07-24" version="1">MKKKSFLAILCSAALFLQLAPAVLANDVLSDATTDEIVTTEEGVTDFGEFGNALWVLKGDTLTLSGKGDLPDDVHDNYVNTISNIYYLDKIVYYPWMKYRSVVKHLVIENGIQDIPKAAFAYFYALETVDFPSTPITIRDHAFYAKDFDIRDGLVAKTSYDIPDNVTLEGYNIFTSDDGGSNSYRRVKINANRYSPTYYYCLNHPCYIFTETGIASEAITSGTCGENATWNFDFDTKTLYIEGKGATESISDDATLKGIEWIQPSIEKVVYGEGITEIAPFSAQKINTHIVYNEPLQDITELVLPSTLTTIDDYAFAFYLNVKDVQIPYGVTTIGNNAFSDCYKIENVVLPGTVKKIGNYAFSVCDSLNSITIPASVTEIGKSLAHSYSGVSIFGYTGSTAESYAKENNYAFYSLSASVGDVNEDGVVNLMDAIALNKYLAGMISLTDNQLVAANCNIADGTSNINEDDVTSLMRFVINLIPDLPVSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXL8</accession>
    <name>R5UXL8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pyridoxal kinase</fullName>
            <ecNumber evidence="1">2.7.1.35</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00816</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.35" evidence="1"/>
    <dbReference type="EMBL" id="CBAR010000039">
        <property type="protein sequence ID" value="CCZ83023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00816"/>
    <dbReference type="EnsemblBacteria" id="CCZ83023">
        <property type="protein sequence ID" value="CCZ83023"/>
        <property type="gene ID" value="BN566_00816"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008478">
        <property type="term" value="F:pyridoxal kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009443">
        <property type="term" value="P:pyridoxal 5'-phosphate salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01173">
        <property type="entry name" value="pyridoxal_pyridoxamine_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013749">
        <property type="entry name" value="PM/HMP-P_kinase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004625">
        <property type="entry name" value="PyrdxlKinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10534">
        <property type="entry name" value="PTHR10534"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08543">
        <property type="entry name" value="Phos_pyr_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phos_pyr_kin" evidence="2">
        <location>
            <begin position="71"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="281" mass="30740" checksum="9D56690115FEBB0C" modified="2013-07-24" version="1">MLKRIVTIQDISCFGKCSLTVAHPILCAMGLETAVIPTAVLSTHTYQFKDYTFRDLTSDIPAIATHWKSLNLQFDGIYTGYLGSTEQVQIVSDFFDTFRNEHTSIIVDPVMGDAGKLYAGFTADFVQKMKALCKKADIIAPNMTEAALLLDRPYRTDYTEAEIQELLKELTDLGAPTVVLTGVQYADGRHGAVAYDSQSNQYHAAFRKHIGGYFAHGTGDIFSSLLSGAIVKGASLEKAVSLAVNLTTDCIEATLPDKDTYWYGVSFESCMGKIAQEAAEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXS7</accession>
    <name>R5UXS7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aminoglycoside phosphotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00910</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000060">
        <property type="protein sequence ID" value="CCZ83123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00910"/>
    <dbReference type="EnsemblBacteria" id="CCZ83123">
        <property type="protein sequence ID" value="CCZ83123"/>
        <property type="gene ID" value="BN566_00910"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="APH" evidence="1">
        <location>
            <begin position="32"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="246" mass="28327" checksum="13CBD43985C309BA" modified="2013-07-24" version="1">MRVLSEQPNKKSYQDGNLRLKVFGASFKKSDILNEALNQARVEETGLQIPKLLEVKQIDGQWTIVTEFIEGETLESLMAAHPEKMTEYLEQFVALQSEILSKRAPLLNRLKDKMNRKIRESGLDATTRYELHMRLNGMREHEKVCHGDFNPSNVIVTPNGDWYVIDWSHVTQGNASADAARTYLLFCLNGKEDVAEQYLQIFCEKNDIAKQYVRSWMPIVAASQLGKGNESEREFLLRWANVVQYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXY8</accession>
    <name>R5UXY8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Probable endonuclease 4</fullName>
            <ecNumber evidence="7">3.1.21.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Endodeoxyribonuclease IV</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Endonuclease IV</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">nfo</name>
        <name evidence="9" type="ORF">BN566_00971</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic (AP) sites, generating a 3'-hydroxyl group and a 5'-terminal sugar phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products.</text>
            <dbReference type="EC" id="3.1.21.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 3 Zn(2+) ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the AP endonuclease 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.21.2" evidence="7"/>
    <dbReference type="EMBL" id="CBAR010000069">
        <property type="protein sequence ID" value="CCZ83188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00971"/>
    <dbReference type="EnsemblBacteria" id="CCZ83188">
        <property type="protein sequence ID" value="CCZ83188"/>
        <property type="gene ID" value="BN566_00971"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008833">
        <property type="term" value="F:deoxyribonuclease IV (phage-T4-induced) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00019">
        <property type="entry name" value="AP2Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00152">
        <property type="entry name" value="Nfo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001719">
        <property type="entry name" value="AP_endonuc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018246">
        <property type="entry name" value="AP_endonuc_F2_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21445">
        <property type="entry name" value="PTHR21445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00518">
        <property type="entry name" value="AP2Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="SSF51658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00587">
        <property type="entry name" value="nfo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00730">
        <property type="entry name" value="AP_NUCLEASE_F2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00731">
        <property type="entry name" value="AP_NUCLEASE_F2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51432">
        <property type="entry name" value="AP_NUCLEASE_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6 7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7 9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="AP_endonuc_2" evidence="8">
        <location>
            <begin position="21"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ83188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="279" mass="30764" checksum="1025F95F9D3EA53F" modified="2013-07-24" version="1">MLRIGCHLPSSKGYLEMGKHAVALGATTFAFFTRNPRGGKAKPIQKEDVAAFLAYAAEHDLQHLVAHAPYTMNLCSADPSIRQFGKDMLADDLQRMEYTPNQYYNFHPGSHVKQGAEVGIAQIAETLNEVLFPEQTTTVLLETMAGKGSEVGRNFSELRAILDSVHLQEKMGVCLDTCHIWDGGYDIAGNLEAVLEEFDKTIGLHRLYAVHLNDSMNPLGAHKDRHQKIGQGYIGLEALKAVVRHPVLKQLPFILETPNDDAGYAAEIALLRKAAEESN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5UY65</accession>
    <name>R5UY65_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ABC-2 type transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01049</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01049"/>
    <dbReference type="EnsemblBacteria" id="CCZ83268">
        <property type="protein sequence ID" value="CCZ83268"/>
        <property type="gene ID" value="BN566_01049"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="177"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="219"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="258"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="346"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="376" mass="41364" checksum="31BC7157561955DB" modified="2013-07-24" version="1">MFWHNLKYEVLSGLRVKDVLFWLILFPIVLATFFKIAFGSIYEKTTLFSTIPMAVVETTEQPVLHTVLDNLESEKEPLFAVTYTDEQTALSLLKEQKVDGILYAGETLSLSFSENGVEQTIIKFFAEQYQIQKAVIQQTMQTAPKKMEAVIAALNDTVVGNQKISMTSGNPDATTNYFYNLLAMVAMFGSLTGMHIAINGQGNLSALGARKCCSPTPKLVSQLACLVGSYILQTVCMSIAVTYLVFILKVQFVGNIGLIYLSAILSGLFGVSLGFSIGSFGTLQQGAKTGIAMTFSMLSCFCSGLMSHGIRPYLAEHVPLFHSINPASLICDLFYCLNMDGNYQRYLVKLGTILGMSVLCTMIGFLLTRRRKYASL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>R5UY70</accession>
    <name>R5UY70_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Sulfate adenylyltransferase subunit 2</fullName>
            <ecNumber evidence="2">2.7.7.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">ATP-sulfurylase small subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Sulfate adenylate transferase</fullName>
            <shortName evidence="2">SAT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">cysD</name>
        <name evidence="5" type="ORF">BN566_01054</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">With CysN forms the ATP sulfurylase (ATPS) that catalyzes the adenylation of sulfate producing adenosine 5'-phosphosulfate (APS) and diphosphate, the first enzymatic step in sulfur assimilation pathway. APS synthesis involves the formation of a high-energy phosphoric-sulfuric acid anhydride bond driven by GTP hydrolysis by CysN coupled to ATP hydrolysis by CysD.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + H(+) + sulfate = adenosine 5'-phosphosulfate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:18133"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58243"/>
            <dbReference type="EC" id="2.7.7.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterodimer composed of CysD, the smaller subunit, and CysN.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the PAPS reductase family. CysD subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.4" evidence="2"/>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01054"/>
    <dbReference type="EnsemblBacteria" id="CCZ83273">
        <property type="protein sequence ID" value="CCZ83273"/>
        <property type="gene ID" value="BN566_01054"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00140">
        <property type="reaction ID" value="UER00204"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004781">
        <property type="term" value="F:sulfate adenylyltransferase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070814">
        <property type="term" value="P:hydrogen sulfide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000103">
        <property type="term" value="P:sulfate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019419">
        <property type="term" value="P:sulfate reduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01713">
        <property type="entry name" value="PAPS_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00064">
        <property type="entry name" value="Sulf_adenylyltr_sub2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002500">
        <property type="entry name" value="PAPS_reduct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011784">
        <property type="entry name" value="SO4_adenylTrfase_ssu"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01507">
        <property type="entry name" value="PAPS_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002936">
        <property type="entry name" value="CysDAde_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02039">
        <property type="entry name" value="CysD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAPS_reduct" evidence="4">
        <location>
            <begin position="27"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="277"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="299" mass="34597" checksum="2F7B57C795CF574D" modified="2013-07-24" version="1">MSEFSHLDELEAEAIYIIREVAAECEKPVMLYSIGKDSSVMLHLAMKAFYPEKPPFPFLHVNTTWKFKEMIAFRDRIAKEKGIEMLEYINQDGVKQGINPFDHGSAYTDIMKTQALKQALAKYGFTAAFGGGRRDEEKSRAKERIFSFRNEAQAWDPKNQRPEMWKLYNTKIKKGESIRVFPISNWTEKDIWQYIQREKIEIVPLYFAAERPVVERDGNLIMVDDDRFPLKDGEVPQMKSVRFRTLGCYPLTGGIESTATTLDEIIEETLSSVSSERTSRVIDNEAAGSMERRKREGYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYD9</accession>
    <name>R5UYD9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glyco_hyd_65N_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00828</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000040">
        <property type="protein sequence ID" value="CCZ83035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00828"/>
    <dbReference type="EnsemblBacteria" id="CCZ83035">
        <property type="protein sequence ID" value="CCZ83035"/>
        <property type="gene ID" value="BN566_00828"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004560">
        <property type="term" value="F:alpha-L-fucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016518">
        <property type="entry name" value="Alpha-L-fucosidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027414">
        <property type="entry name" value="GH95_N_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14498">
        <property type="entry name" value="Glyco_hyd_65N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007663">
        <property type="entry name" value="UCP007663"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="SSF48208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyco_hyd_65N_2" evidence="1">
        <location>
            <begin position="7"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="772" mass="86788" checksum="187E0EA08101E774" modified="2013-07-24" version="1">MDSQTTLWYRSPATDFDHALPVGNGRIGGMLFGDPEHEVIKLNEDSVWSGGRRERNNPDAYEGLLEIRKLLAEEHIQAAEAVAFEKMQGVTPNARHYMPLGNLEIHQKIQGKARQYCRSLDLEQALAAVQFNVNEVNYIRELFVSYPDQIMVLHIAADQKGCISLSVGIDGREDDYDDCRPCAPNTILYTGGTGGKHGIGFAAALTGKQTGGTMRTLGGQLVIENADEVTLLLSVATSFYHGDDYEEAARMDVAYAQDCTYEELLYRHLSDYQKLFRRVRLNLPDNSEGNSSLPTDERLQRLRGDEGDHKECKLQIHDGHLAVLYFNYGRYLMIAGSRPGSQPMNMQGIWNEDMHPAWGSRYTVNVNTQMNYWPAEVCNLSECHLPLFDLIARMQDPGRKTAKEMYHCRGFVCHHSTDIWGDTAPQDLCKPATIWPTGAAWLCLHIFEHYLFTQDRDFLSAHYDLMREAALFFVDYLMENEKGELVTCPSVSPENTYRTKSGETGSLCMGAAMDSEIITVLFQDVIESSRILQRDEAFAQELSDLLKKLPKIKIGKYGQIQEWSEDYAEVDIGHRHISQLFALYPAHLIDPHQTPQLSKAARATLIRRLIHGSRNHTGWSRAWIINMWARLLDGRMAYENLQQLLTWSTNPNLLDSHPPFQIDGNFGGLAAIAECLLQSHADELNLLPALPPEWTTGSVSGLRARGGFEVGMQWENGKLTTAAILSLNGNPCRLRCNAVASVTCEQKPVEALQDGETIQFETVAGHTYTVRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYE9</accession>
    <name>R5UYE9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">DNA modification methylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_01124</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the N(4)/N(6)-methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000092">
        <property type="protein sequence ID" value="CCZ83348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01124"/>
    <dbReference type="EnsemblBacteria" id="CCZ83348">
        <property type="protein sequence ID" value="CCZ83348"/>
        <property type="gene ID" value="BN566_01124"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006306">
        <property type="term" value="P:DNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002941">
        <property type="entry name" value="DNA_methylase_N4/N6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002295">
        <property type="entry name" value="N4/N6-MTase_EcoPI_Mod-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01555">
        <property type="entry name" value="N6_N4_Mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00506">
        <property type="entry name" value="D21N6MTFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0680">Restriction system</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N6_N4_Mtase" evidence="5">
        <location>
            <begin position="76"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ83348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="636" mass="73273" checksum="3CC0CAB0BBE50F56" modified="2013-07-24" version="1">MEMDGQLTFEFSKRPTIKGFPELRWTGKRPYESTQYYPAQLKERYGEDVSGWINKIFWGDNLQVMSHLLKKYRGQVDLIYIDPPFDSKADYKKSIKLKGQVVTGDSNSFEEKQYGDIWTNDEYLQFMYERLTILRDLLSETGSIFLHCDYHRNSYLRIIMDELFGNGGQDGKGSGFKNEIIWQRTGAHNDAGKYGIVHDTILWYTKTPTYKFTMEMIPLTDDHISSRFKTIEPETGRRFYPGPITAPGDGPSRIFRGKELFPPAGRHWSYSQENIDELERQNRIYYSSTGVPYLKEYADEYTEQGRRLQSIWTDILPSKTGSEIVGYPTQKPEKLLERIIKACSEPGDLVFDCFMGSGTTQAVAMKLGRRFLGADINLGAIQTTTKRLIGIAQELSQQTLDADDVKYTGFEVYNVNNYDFFRNPVEARDLIIQALEIQPFEQSNVWDGEKDGRMVKIMPVNRIATKADLEELKANLPYKTYEKRKEENPSQPVEKITIVCMGHEPDLAASFQKELSDYKLDIEIVDILRDKSELELKREAEAEVVREGGKLVIRAFYPMNLLQKLSLQKEYVEDWRQLVESIMIDWNYDGVVMQPAVTDIPDKKSLVLGVYDIPDDAGTIKVKITDLLSESLEVEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYI9</accession>
    <name>R5UYI9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01159</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000093">
        <property type="protein sequence ID" value="CCZ83383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83383">
        <property type="protein sequence ID" value="CCZ83383"/>
        <property type="gene ID" value="BN566_01159"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="155" mass="17597" checksum="1474B49A36E5AE7E" modified="2013-07-24" version="1">MSRIESEELSMMDIEWYGVDRKGKIAVFCSAGIANVPEFVCADKERQERLIALFEKLPILSGVSFCFACSEKNLLPFQVAETFSGKGFFYYDSDDHSKSHKNVGVYQKYYTISSKPTNPLFYAELPASIQLLLKDNFLPIDDFQTCNIVAVENAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYJ9</accession>
    <name>R5UYJ9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_01169</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000094">
        <property type="protein sequence ID" value="CCZ83393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01169"/>
    <dbReference type="EnsemblBacteria" id="CCZ83393">
        <property type="protein sequence ID" value="CCZ83393"/>
        <property type="gene ID" value="BN566_01169"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="11"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="47"/>
            <end position="54"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="233" mass="25651" checksum="986F619751E2C2CA" modified="2013-07-24" version="1">MQRQAGDADMLKLEHIEKAYQQGKNIVPVLHDITFHVAHGEFVAIMGPSGSGKSTLMNLIGLLDTPTSGSYFFDGTEVSCCSENMLSQIRNEKIGFVFQNFNLLPRQSALENAALPLLYAGVKPKVRRERAREALEKVGLADRVSFLPTQLSGGQKQRVAIARAMINHPKLLLADEPTGALDTASGEQVMELFHELHKTGVTIVMITHELEIAQHAERILTIRDGRLGEEQVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYR7</accession>
    <name>R5UYR7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sporulation factor SpoIIGA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00963</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000069">
        <property type="protein sequence ID" value="CCZ83180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00963"/>
    <dbReference type="EnsemblBacteria" id="CCZ83180">
        <property type="protein sequence ID" value="CCZ83180"/>
        <property type="gene ID" value="BN566_00963"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030436">
        <property type="term" value="P:asexual sporulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005081">
        <property type="entry name" value="SpoIIGA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03419">
        <property type="entry name" value="Peptidase_U4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="271" mass="30599" checksum="B519A8B43E2E2145" modified="2013-07-24" version="1">MQTIYIDVLLFFNFCVNYFLLRGTAFLTHTPCRIPRCLLSALLGSFSALVILLPPLPLAVNLLIRLGTAAGMNGVLCFGLSRRTMLRQTIWFFCCSMLFAGFLMFLQQSTNSRRIFLGNQCFYLQLSLPVCIFCTLLAYGVLRLWHAVRMRCRHTDVPYRVYIRIGSETRIQTGLADTGNNLVDPYSGLPIIVCSRQAFSDLLPQCQKKQRHYHYVPYGTVSGTSLMPVFQADEVLLQNETTGARQQVAARIGLADAQEQAIFHPNLLQTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYS4</accession>
    <name>R5UYS4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter related</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_00973</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000070">
        <property type="protein sequence ID" value="CCZ83190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00973"/>
    <dbReference type="EnsemblBacteria" id="CCZ83190">
        <property type="protein sequence ID" value="CCZ83190"/>
        <property type="gene ID" value="BN566_00973"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="235" mass="26162" checksum="B49EBA88E97AEAEC" modified="2013-07-24" version="1">MLTFEHVRKNYDSKRVLEDCSISFEPGKVYALVGPNGTGKSTLMKAAAGLVKINGGTLTYKGQPIGTESKKHIAYMSTEPFYYDYMRIRDVKAFHKDFFADFDADLFDRLLTFMELTPDLKVRALSSGMAAKLKIAVTLSRRAEVIMLDEPLNGIDLIGRDQIIHTIIQATGSGATFIISSHLFDELEPIVDNVVMMKGGKVILEGELEAIRAEHGKSISDLYREIYADTAVPQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYS9</accession>
    <name>R5UYS9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00978</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000070">
        <property type="protein sequence ID" value="CCZ83195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00978"/>
    <dbReference type="EnsemblBacteria" id="CCZ83195">
        <property type="protein sequence ID" value="CCZ83195"/>
        <property type="gene ID" value="BN566_00978"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039522">
        <property type="entry name" value="RING_finger_1_prok"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14446">
        <property type="entry name" value="Prok-RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="180"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="259"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="315"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="344" mass="39247" checksum="157D626C762E554F" modified="2013-07-24" version="1">MADYTGQSCFVCQKPFEPEDDVVVCPECGTPYHRACWQHYGHCINVHLHENGGSWKPVEIPLPESETASETRRCPRCGTENPVHQAFCGHCGMILEENGQQQTAQQNQQTWQQSAQDICCGLDPNEDYEGERLEDLANFVRTNTIYYVPAFKKFRETGSKLTMNLISAVFPQLYFASRKMWGMTFLIISVLLLLDLPSELYSFVTNNDSILSQLHSMQQSMNSMGGMGSMYNNFLQVMTEQIQSFCTALLPYETALHTMYVICMYLSFAVRVLLFAFSNYIYYRFVLRKVHRIRAEGLPEGMTKDRLRMAGGTNYWFVLIAILAQYAVTVLVTALFLLGISMAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYV6</accession>
    <name>R5UYV6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01257</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000117">
        <property type="protein sequence ID" value="CCZ83488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83488">
        <property type="protein sequence ID" value="CCZ83488"/>
        <property type="gene ID" value="BN566_01257"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="49" mass="5879" checksum="6CECBB5901743000" modified="2013-07-24" version="1">MPKGQPNKSELTVFFYLFLYRRAMKQKYTLHSDFIHKVAPIRSAFHTSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYW5</accession>
    <name>R5UYW5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative sortase SrtB family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01016</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000081">
        <property type="protein sequence ID" value="CCZ83235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01016"/>
    <dbReference type="EnsemblBacteria" id="CCZ83235">
        <property type="protein sequence ID" value="CCZ83235"/>
        <property type="gene ID" value="BN566_01016"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030435">
        <property type="term" value="P:sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013486">
        <property type="entry name" value="SpoIID/LytB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013693">
        <property type="entry name" value="SpoIID/LytB_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30032:SF4">
        <property type="entry name" value="PTHR30032:SF4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08486">
        <property type="entry name" value="SpoIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SpoIID" evidence="1">
        <location>
            <begin position="136"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="317" mass="34014" checksum="5AB51A73269640E1" modified="2013-07-24" version="1">MAAETDKNWKKAQISDYDTSLSLVEYQEVDETEAEKMQIATATGELATSGTVGSSAPDAAVSLATDLQPGEFGFTTYGYGHGCGLSQNGANFYATYGGYDYQAILFHYYPGTTLMQVDIPDSVTAGGRTGSVLDIISMLVYNEMGSTMAPEAMKAQAVAAFTYVMNPKANNNSLICKDNPPQNVIDAVQSVLGQALYYNGDYALTVYSASSGGYTAAASDVFGVNYDYLISVPCEYDAEYDPHYGTVVYFSEAEIRSRLQSAYNITLSDNPANWISLEIGGGGYISHVTIDGQKTVTGESLRSVLGLKSDRYNYAIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYX7</accession>
    <name>R5UYX7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ferrous iron transporter FeoB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_01031</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000081">
        <property type="protein sequence ID" value="CCZ83250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01031"/>
    <dbReference type="EnsemblBacteria" id="CCZ83250">
        <property type="protein sequence ID" value="CCZ83250"/>
        <property type="gene ID" value="BN566_01031"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015093">
        <property type="term" value="F:ferrous iron transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011640">
        <property type="entry name" value="Fe2_transport_prot_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041069">
        <property type="entry name" value="FeoB_Cyto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011642">
        <property type="entry name" value="Gate_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07664">
        <property type="entry name" value="FeoB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17910">
        <property type="entry name" value="FeoB_Cyto"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07670">
        <property type="entry name" value="Gate"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="296"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="347"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="391"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="468"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="500"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="528"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="555"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="597"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="628"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="domain" description="FeoB_Cyto" evidence="4">
        <location>
            <begin position="10"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="Gate" evidence="3">
        <location>
            <begin position="304"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="FeoB_C" evidence="2">
        <location>
            <begin position="408"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="domain" description="Gate" evidence="3">
        <location>
            <begin position="465"/>
            <end position="594"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="662" mass="72166" checksum="0AC72B319D7620E1" modified="2013-07-24" version="1">MVPQHKFSGCVEHAIAHIEEAALHNMPEAQQRWYAIKVFERDEKVLEQLKIDQSTLTHIEGDIKSAEEEMDDDAESIITNERYVYIASIIKDCLKKKKVGMTTSDKIDKIVTNRFLGLPIFAVIMFLVYYISMVTVGSAATDWANDGLFGDGWHLLGIGTSSYNDAADEYGDTNAIIDGYVAYLGEQGADTEALEGYIDAEADTYDGEAAKDAILAFEKDYHADFSYDVEDEETLEVTTETATMDDLNTAADLFAAGEPDPADYGVWVPGIPVLIGNGLEAINCADWLQGLILDGIVAGVGAVLGFVPQMLVLFILLAILEACGYMARIAFVMDRIFRKFGLSGKSFIPILIGTGCGIPGIMASRTIENERDRRMTIMTTTFIPCGAKQPFIAMIAGAIFGGSAWIATGAYFIGMAAIIISGIMLKKTKMFAGDPAPFVMELPAYHIPTVGNVLRSMWERGWSFIKKAGTIILLSTIFVWFTSFFGWVDGSFQMLTEDQMEFSILAHIGKAICWIFAPLGWGDWQATVAAVTGLVAKENIVATMGILYGGGDGTVYANIAAAFTAVSGMSFLVFNLLCAPCFAAMGAIKREMNSAKWFWFAIGYECGFAYVIALIINQLGKLFTGDVNVFGLIVAIIAIALIIFMLVRPYKESNKLGVKVKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZ17</accession>
    <name>R5UZ17_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pseudouridine synthase</fullName>
            <ecNumber evidence="3">5.4.99.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_01312</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the pseudouridine synthase RsuA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.-" evidence="3"/>
    <dbReference type="EMBL" id="CBAR010000121">
        <property type="protein sequence ID" value="CCZ83543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01312"/>
    <dbReference type="EnsemblBacteria" id="CCZ83543">
        <property type="protein sequence ID" value="CCZ83543"/>
        <property type="gene ID" value="BN566_01312"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120159">
        <property type="term" value="F:rRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1560">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042092">
        <property type="entry name" value="PsdUridine_s_RsuA/RluB/E/F_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000748">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluB/E/F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018496">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020094">
        <property type="entry name" value="TruA/RsuA/RluB/E/F_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00093">
        <property type="entry name" value="TIGR00093"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01149">
        <property type="entry name" value="PSI_RSU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PseudoU_synth_2" evidence="4">
        <location>
            <begin position="56"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="233" mass="25879" checksum="958AE021F21B0825" modified="2013-07-24" version="1">MLATAPAARKAEALIAAGKVKLNGHPVKLGDQATRADLITVNGEAVRIPRKKVKRYLMLYKPRGYVTTTSDELDRRCVMDLLDGVEERVYPVGRLDKDSEGLLLLTNDGEFANNIMHPSRHISKTYRVTVRPDISEEQLIALSSGVELDGKMTLPANVVVKTKEPNRVVLLMTIQEGRNRQIRRMCEALGLEVARLRRTSIGPLKLGMLKPGTYRDLTAEELRAIRNAIGGDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZ72</accession>
    <name>R5UZ72_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">YkuD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00150</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000146">
        <property type="protein sequence ID" value="CCZ83623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83623">
        <property type="protein sequence ID" value="CCZ83623"/>
        <property type="gene ID" value="BN566_00150"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16913">
        <property type="entry name" value="YkuD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005490">
        <property type="entry name" value="LD_TPept_cat_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03734">
        <property type="entry name" value="YkuD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="YkuD" evidence="2">
        <location>
            <begin position="129"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="291" mass="31251" checksum="E0A88B04D89E93B9" modified="2013-07-24" version="1">MIRPNTPEQKRMLILLSIVIGLLLIGIGCFGVLLHQKSAQEPQHSTTVSIESTDSITTELEMKTQATTVSTSVTTTTTATTISTAMETTTIPVCAELATILDNNGYTLSDLGDSKQLIAVVSSGCSAVVYGFSAGEQGWKMDFVSNGCVGTNGVSANSREGISCTPKGLFSLGFAFGTDPLTDLSIPYRQLNENCYWVDDPASPVYNQWQETTEINWNSAEHLIDYASSYHYAVVVNYNCDPVVPGAGSAIFLHCTAGASSTAGCIAVPDSQMWDILHWLREEDLPLILTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZF8</accession>
    <name>R5UZF8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01463</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000158">
        <property type="protein sequence ID" value="CCZ83728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01463"/>
    <dbReference type="EnsemblBacteria" id="CCZ83728">
        <property type="protein sequence ID" value="CCZ83728"/>
        <property type="gene ID" value="BN566_01463"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13416">
        <property type="entry name" value="SBP_bac_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004406588" evidence="1">
        <location>
            <begin position="29"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="431" mass="48486" checksum="A433CB10C39D9BB5" modified="2013-07-24" version="1">MKKRNAICSLILAGVSLSTLLLQGCGSGKTSDGKIEVELLQYKPEAVAAFEAMEERFNATHDNIHLTIESPNEAVTVLKTRLIREDYPDIIGIGGDINYSNFLDANLFMDISDFEGISDVKQAYLDMEKELELVPKDGTYALPYVANCAGILYNKDIFEEHGWSIPETWDEFTSLCEEIQSAGMTPLYFGFKDTWTCLAPWNALAVGLAEPDTCSEVNAGNTTFTDAYRGVAEKMKVLLDYAEPNPYAYSYNDACTAFARGESAMYAIGSYAVPQIQSVNPDINIDSFTFPANDKEEDNVLNSGVDLQFCVMKETKNKEAVYEVLKFLCEDETIQIYLDEQNAVPCKEGDFTLPSMLNGMQSYIQEGRMADFQDHHYPSEMSVDAMIQTFLMDDSSNAVDTFLSRFDKEWKRYNRDLIAKVKKYQEEKGEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZG2</accession>
    <name>R5UZG2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Lysophospholipase L1 and related esterases</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01468</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000159">
        <property type="protein sequence ID" value="CCZ83733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83733">
        <property type="protein sequence ID" value="CCZ83733"/>
        <property type="gene ID" value="BN566_01468"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013830">
        <property type="entry name" value="SGNH_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13472">
        <property type="entry name" value="Lipase_GDSL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SGNH_hydro" evidence="1">
        <location>
            <begin position="6"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="212" mass="23438" checksum="A3150310202C1FA4" modified="2013-07-24" version="1">MKTILCFGDSNTYGYQPETKKRYDETTRWTCLLQQAIQPYGWRVVEEGLCGRTTNLNGIRRENRNGAAVLPMLLESHAPLDVVILMLGTNDCKTQFHATPESIGAGIEALIAQVRAFDEQLPILLVSPIHLAADIAEKDPAFSADSVPISYALKSVYAAIAEREHCMFFAASDYAEPSDADGEHLDAEGHQKLTKQMLNALQPLLCGKEQEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZG5</accession>
    <name>R5UZG5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01473</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000159">
        <property type="protein sequence ID" value="CCZ83738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01473"/>
    <dbReference type="EnsemblBacteria" id="CCZ83738">
        <property type="protein sequence ID" value="CCZ83738"/>
        <property type="gene ID" value="BN566_01473"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="493" mass="55390" checksum="691FBB96E1799F1E" modified="2013-07-24" version="1">MVDIVKMQRYLLRLDTLQGEDILQRADLNQDHVVNVTDLMMLKHCVLYEDFLEIWEEIADTTTVTSDTTAPVTTTTETTTTTTDAEDATFLTPSIQAVKASLPSQGDAKLVIFYVDFPDCKYTTDLSAEQIQEIAFGGADTSDSNYPFDSMRAFYERSSKGTMHLDGQVFRYTTKENRSAYDTDKNKLARECYDAFNDSVDFSQFDSNNDGVIDATLFSVPTAAGNDNWWPCAGPIGDSAYTVDGVTIGHIITGNAQIESETNYNNFNSSYLHEMGHCMGLPDYYLYNSDDFEGMKGNAGTELMDADAASDFGCFSKLMLGWYQKNQVSVYDSSKGTQTFQLQNAQTKNGNCVIIPYGKLDGRYFSEYLMIEYVTEDDNNSYINSHMSYWQSIDSGIRVYHIQADLQEDYWYTYFKYQNGSEYTNNDNDGIRLIRLANEQEGGSVFKTGDVIDGTISGFHWYDANEQETIDPGVTVTVGDLKDGAYTITISNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZG9</accession>
    <name>R5UZG9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01478</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000161">
        <property type="protein sequence ID" value="CCZ83743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83743">
        <property type="protein sequence ID" value="CCZ83743"/>
        <property type="gene ID" value="BN566_01478"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="84" mass="9311" checksum="B3210A78B3C45DDF" modified="2013-07-24" version="1">MVKGVHKKVIEVHQPDSAYFEKAIFYLRPGITQLPPELAQAAAREYLQELEPNPLPPFWKRTLPLFCSAVLSAAAACAVCFAVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZP3</accession>
    <name>R5UZP3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00131</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000113">
        <property type="protein sequence ID" value="CCZ83475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83475">
        <property type="protein sequence ID" value="CCZ83475"/>
        <property type="gene ID" value="BN566_00131"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="133" mass="14254" checksum="CE4DBE8ACBB14544" modified="2013-07-24" version="1">MTLDEKIDYVNQLPAEERSNYISNLTTEERNSFIKQLPVSDKAAVLNEFLGVGDSLGLHFTVDEMTNDSVVVSARDKDGKLIDVSAMGVTVEETGKSYTTPITIAALLAGGGICGVSAIIFAAGKKKRNEEQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZS5</accession>
    <name>R5UZS5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Bacterial membrane protein YfhO</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01588</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000167">
        <property type="protein sequence ID" value="CCZ83853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01588"/>
    <dbReference type="EnsemblBacteria" id="CCZ83853">
        <property type="protein sequence ID" value="CCZ83853"/>
        <property type="gene ID" value="BN566_01588"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018580">
        <property type="entry name" value="Uncharacterised_YfhO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38454">
        <property type="entry name" value="PTHR38454"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09586">
        <property type="entry name" value="YfhO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="187"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="237"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="306"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="336"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="362"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="389"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="427"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="457"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="754"/>
            <end position="776"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="800" mass="90666" checksum="61F76295AB053E02" modified="2013-07-24" version="1">MRFSIRKSISSHSLFHNYLFAFLLGFGALLIVYLPAMCLEHGYFLYYGDYNSQQLPFYSLANDAVRSGSFGWNWYTDLGANFIGSYAFYLLGSPFFWITTILPRSWMLYALPVLLSVKHGIAAMTAYAYIQRFVRSRNAALIGGLLYAFSGFQLFNLFFNHFQDVTAFFPLMLIAMEESINQNRKGVFALAVALMGCINYFFFTGQAVFLVLYFIVRCFSKDFHATPKKFFRLALEAIIGVLLACFLLLPSALAILANNRITSRLYGMDMLAYNDRTRIWRIIESFFLIPDVPARPNLFSSNDAKWASIGGYLPLFSMVGVLSFCKYKDGHWAKRLVLLCTICAFIPILNSCFYALNGSYYARWFYMPICIMALMTAYVLDNTTISAKYGLGISALMMAVFGVISILPTKNGDKVTWGKFAEYPLHFYLVLGICILSLVATAFLFHRRRAGKPILAMALPMTVAACTFCTMTVVYFGAYTPQRAEEYIDKAIDKEGDVKIAVSEDDFFRIDISKDRDNYPMFWGLPCIRAFHSIVPASIMEFYPEIGVTRDVASRPERSCKAIRYLFSVRYYFDEVTPDNLEPEGIDLPGFSYYDTQNGFYIYKNDYALPMGFTFDTYVSRSQWETYSESDRCNLLLRALVLEDDTVETYQNWMQKLPDAVHQMSDEDLLAECTERTKTACDSFTYNSKGFHATISTDHENLVFFSVPWEEGWSAEVNGKPVSIEKADVGFMAVPVPAGDSEITFTYHTPGLKAGALLSLGGLVLFAGYLTVAGIYNKRHPNPKTESICCVDYSTEETAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZU2</accession>
    <name>R5UZU2_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Transcription termination/antitermination protein NusG</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">nusG</name>
        <name evidence="8" type="ORF">BN566_01602</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">Participates in transcription elongation, termination and antitermination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the NusG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000169">
        <property type="protein sequence ID" value="CCZ83873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01602"/>
    <dbReference type="EnsemblBacteria" id="CCZ83873">
        <property type="protein sequence ID" value="CCZ83873"/>
        <property type="gene ID" value="BN566_01602"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006354">
        <property type="term" value="P:DNA-templated transcription, elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription, termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032784">
        <property type="term" value="P:regulation of DNA-templated transcription, elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031564">
        <property type="term" value="P:transcription antitermination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00948">
        <property type="entry name" value="NusG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006645">
        <property type="entry name" value="NGN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036735">
        <property type="entry name" value="NGN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043425">
        <property type="entry name" value="NusG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001062">
        <property type="entry name" value="Transcrpt_antiterm_NusG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30265">
        <property type="entry name" value="PTHR30265"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30265:SF2">
        <property type="entry name" value="PTHR30265:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02357">
        <property type="entry name" value="NusG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00338">
        <property type="entry name" value="NUSGTNSCPFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00738">
        <property type="entry name" value="NGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82679">
        <property type="entry name" value="SSF82679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00922">
        <property type="entry name" value="nusG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 5 6" id="KW-0889">Transcription antitermination</keyword>
    <keyword evidence="3 5 6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1 5 6" id="KW-0806">Transcription termination</keyword>
    <feature type="domain" description="NGN" evidence="7">
        <location>
            <begin position="4"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="175" mass="19386" checksum="DDA1E85F2C232223" modified="2013-07-24" version="1">MSETAKWYVIHTYSGYENKVAQDIEKVVENRKLHDMILEVRVPTEKVTEITDGKIREVERKTFPSYVLLKMIYTDDTWHVVKNIRGVTGFVGPGSEPTPLSEQEVQALGVDLGTTIEVNYAVGDTIMISGTAMDGFVGVVQNINMEEGTVDVLVSMFGKETPATVALNQVVLMDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZY3</accession>
    <name>R5UZY3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HTH dtxR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_01299</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DtxR/MntR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000121">
        <property type="protein sequence ID" value="CCZ83530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01299"/>
    <dbReference type="EnsemblBacteria" id="CCZ83530">
        <property type="protein sequence ID" value="CCZ83530"/>
        <property type="gene ID" value="BN566_01299"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.60.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001367">
        <property type="entry name" value="Fe_dep_repressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036421">
        <property type="entry name" value="Fe_dep_repressor_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022687">
        <property type="entry name" value="HTH_DTXR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022689">
        <property type="entry name" value="Iron_dep_repressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02742">
        <property type="entry name" value="Fe_dep_repr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01325">
        <property type="entry name" value="Fe_dep_repress"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00529">
        <property type="entry name" value="HTH_DTXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47979">
        <property type="entry name" value="SSF47979"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50944">
        <property type="entry name" value="HTH_DTXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH dtxR-type" evidence="5">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ83530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="131" mass="14823" checksum="8A0D4C0717B88CB1" modified="2013-07-24" version="1">MKIQESAENYLETILILQQRIGEVRSIDVVNELSYSKPSVSIAMKNLRNAGLVTMDESGYLHLTAAGRKIAETMYERHTLLSDWLIYLGVDPQKAVEDACRMEHILSAESFHAIKQHVLANQEQTENGRHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZY6</accession>
    <name>R5UZY6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">SseB protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01304</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000121">
        <property type="protein sequence ID" value="CCZ83535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01304"/>
    <dbReference type="EnsemblBacteria" id="CCZ83535">
        <property type="protein sequence ID" value="CCZ83535"/>
        <property type="gene ID" value="BN566_01304"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027945">
        <property type="entry name" value="SseB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009839">
        <property type="entry name" value="SseB_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07179">
        <property type="entry name" value="SseB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14581">
        <property type="entry name" value="SseB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SseB" evidence="1">
        <location>
            <begin position="14"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="SseB" evidence="1">
        <location>
            <begin position="181"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="SseB_C" evidence="2">
        <location>
            <begin position="319"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="421" mass="47682" checksum="B14049716D038BCD" modified="2013-07-24" version="1">MAKLKKPTVKNPQLVEALAELKEHSTQGAVKNLMEALKGAQLIAPVIIDNMPEDLDLSDGKQFQTNAKFTMLQQKDGSRYFPAFTDWLEVLKWRNDPEINTVVMTVEQYANLMQRSGEASGIVINPSEQGYLIDRPHLAQLTGLTLPPAASEYRNEPLPEPTLNPKVAFADASTLSNPQLVGAIAAFRKKSTQETELPMIDALFRSQVIAPVQLINAEQDLNMEDGEKHQTQIKFVMVQNNDKKFFPAFTDMEELNRWQNPPEYRTMIIPFLQYAAMFQQNGDAEGIVVNPFREGESVAMPKANVIDLAKRFVHYQRQQQQNRVQLFDLKDKPIDLMKELSAHFEEVPEVHAAYMTGMRVGNKESIMLVLDCDQVEDTKSLFQPAAQIVNRHTNGNPPGIITTQHDMSKSILERTKPFYQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0C5</accession>
    <name>R5V0C5_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN566_01456</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="2"/>
    <dbReference type="EMBL" id="CBAR010000155">
        <property type="protein sequence ID" value="CCZ83720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01456"/>
    <dbReference type="EnsemblBacteria" id="CCZ83720">
        <property type="protein sequence ID" value="CCZ83720"/>
        <property type="gene ID" value="BN566_01456"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000212">
        <property type="entry name" value="DNA_helicase_UvrD/REP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038726">
        <property type="entry name" value="PDDEXK_AddAB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070">
        <property type="entry name" value="PTHR11070"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12705">
        <property type="entry name" value="PDDEXK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDDEXK_1" evidence="9">
        <location>
            <begin position="749"/>
            <end position="1088"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ83720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="1123" mass="128555" checksum="82C7D8622B423752" modified="2013-07-24" version="1">MLHIIAGGSGTGKSTLLMQKIRQLLPQEPDCIILVPEQFSYEFDKKLYHFLGATAFNQLETHSFTSISRMLFQKYGKHAAQYADATVRKALFLQAVQTVRQNQVLYFFDRQCGKTDFMEHLEQILSVLRRNGTDSSALFETAQQCSGRLRDKLQDVANIYQEYESLLEEQHLQDALTDITEAASIANGADFFLGKTIFIDEFESFTEDEYAMLEVMVGTAADIYITLRVEEQSESADTLFATVQETRNRMLELAKRFHIASDVQFCAETKRFSSADLAYLSKTVFRSGSIAACPEQAQHIHIFEARDAVSELEYVCATIRKQLMQDDTLHCSDIAILTNDLEAYRPIVHHAFSRYELPFYLDTAVSVLHHPFSVSLLTLLELVQQRHLQTDCLLRYAKAGFSGCDWLALSKLENYCYQWNIKGKTWEVPFPVPEKESLQEEQAEMEQTRQQLVTPILELKQAFFNCNTGADYCNALYEHIEKTKLLEQLSTSCQSENAEKTIAQQQDAKYIWEQFLSILDTIYRLYSKKPMTTADFCQTISYLFQTITYATPPRTLDAVLIGQTGRSRLRDPKIVFVIHCSEGAFPATGLAADLFSEREYRLMEEKQLVIRKSLQKILADERLAVYKTISAPAQELYLTYPLVDTANQKSYPSFLLGQIPEWFPNGKQLLQTEAQIPLSYYATTMQSAYYHYVRNFSEHSAAIAAVQHVLQQQPVYAQKIAYLQSVHQQMDFSITEPKHMEQLLGKQLHMSASKLETYRLCPFQFFCGSALHLYARKKIQLDPVNQGNLIHYCLEQMLRNHTRDVFLALTPEELEKKLQYWVNQYWNTALGGDFSKDPRSYAVYAEVQRQLLITLLHVQAELRQTRFYPHYLELPIRSHTAFSPLKALTEDGHSLQIEGIIDRVDTCELDGKTCVRIIDYKSGTKSFSFGNLRYGLDMQMLIYLFSLIYNQTPLQNAKIGGVLYMPAGGVKTNQERGNAKSKQELEQDAYRMSGLLIDDAERIRCMEPDGKGIYIPAKLKAGTSGASELITGKNDVYLSESAFHALYRYVMETLKQTAQQIYQGDIAANPLILPKKTVCKYCNYQDICGNLQQQPCRTTEPDAQEAMLEQLKTWEESEHGMDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0I3</accession>
    <name>R5V0I3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Flavodoxin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01824</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000186">
        <property type="protein sequence ID" value="CCZ84098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01824"/>
    <dbReference type="EnsemblBacteria" id="CCZ84098">
        <property type="protein sequence ID" value="CCZ84098"/>
        <property type="gene ID" value="BN566_01824"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12682">
        <property type="entry name" value="Flavodoxin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="1">
        <location>
            <begin position="4"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="170" mass="18846" checksum="BD83FFF3442F4CD7" modified="2013-07-24" version="1">MKSLIVYDSLTNGNTKRIAEMLQQATQADIAEIQTVTPYTGGYDAVVAQGQEEVNRGYEPEIQPLSVSVADYDRIFIGTPTWWYTMAPAVRTFLHQQNFSGKTVIPFMTNGGWPGHTIADMKIACRGAAFAHEKEIQFDSNGGDHLETPMQEIKAWIAAICADETQEETV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0P3</accession>
    <name>R5V0P3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Bacterial capsule synthesis protein PGA_cap</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_01570</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CapA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000166">
        <property type="protein sequence ID" value="CCZ83835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01570"/>
    <dbReference type="EnsemblBacteria" id="CCZ83835">
        <property type="protein sequence ID" value="CCZ83835"/>
        <property type="gene ID" value="BN566_01570"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07381">
        <property type="entry name" value="MPP_CapA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019079">
        <property type="entry name" value="Capsule_synth_CapA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09587">
        <property type="entry name" value="PGA_cap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00854">
        <property type="entry name" value="PGA_cap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="67"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="PGA_cap" evidence="5">
        <location>
            <begin position="137"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="108"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="24"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ83835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="492" mass="54849" checksum="E8B3BA944D81AF39" modified="2013-07-24" version="1">MENQPFQQKPISARNSGQQRPPQRTTPPRMPQRQPIQTQRPPQQPPRQPQNQNPKKKKKGIGYRRRRNALLILLALLIVIVLVCTRCNSCRSISKGDGVQVNAPTEVTTQDASAATEAASDATEATTEETAKSGTVHVVAAGDHFVQTSVYNSAAAHAENGETYNFAYVYDGVKDLVNGSNADLRIINQETLICDNPDIEISGSNFNFNSPPEAGEAIVDLGFNVVSMCNNHLLDKGVEGLTSCMDYWDRLLQEHSDLLTYGVYRDVDDMNNIRIKEVNGLKVAFLAYTENINGYTVPDDSTIQITLTTQDDVIQEQIERANELADIVVVSAHWGDEDTFEVRDGVKAQAQNMIEWGADVIIGNHPHVPQTMEYITRSDGTQGFVFYAMGNFVSAQTFNINLISEVADFDLVYHEEDKQVTVENVQVHPIITQYDDGSLSNLRLIAYKDYTEELAEEHGLPYAMSGEYGIWNMDKIKEIIDTAIPAEFQKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0X2</accession>
    <name>R5V0X2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01649</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000173">
        <property type="protein sequence ID" value="CCZ83920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01649"/>
    <dbReference type="EnsemblBacteria" id="CCZ83920">
        <property type="protein sequence ID" value="CCZ83920"/>
        <property type="gene ID" value="BN566_01649"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="164" mass="18333" checksum="EAC282680FB0F076" modified="2013-07-24" version="1">MISIRVAEQADLPALLQIYNEEVLHGTATFDLHPKTLAERQVWFEQHNRDNHPLLTAELDGTIAGYASLSAYAEKEAYCSTVELSVYVAKFAQRKGVASALMQEILAIAQIDVRIHSVVSIITGGNAASERLHEKFGFQRCGMLKEMGKKFGQYLDVVYYQLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0Z5</accession>
    <name>R5V0Z5_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 4">Thioredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_01669</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the thioredoxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000174">
        <property type="protein sequence ID" value="CCZ83940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01669"/>
    <dbReference type="EnsemblBacteria" id="CCZ83940">
        <property type="protein sequence ID" value="CCZ83940"/>
        <property type="gene ID" value="BN566_01669"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005746">
        <property type="entry name" value="Thioredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000077">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01068">
        <property type="entry name" value="thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="6">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="5">
        <location>
            <begin position="31"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00020570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000077-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ83940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="106" mass="11743" checksum="6521C6F7F7B8CBF2" modified="2013-07-24" version="1">MAVTTVTKDNFEAVVLQAEKPVLIDFYATWCGPCRMVSPLVDEIAEEYQNYQICKVDVDESPELAQKFGVMSIPTLVVLKQGKVITQNVGALPKNAILDLLEQAEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5V119</accession>
    <name>R5V119_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 11">Cytidine deaminase</fullName>
            <ecNumber evidence="6 11">3.5.4.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">Cytidine aminohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BN566_00032</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 11">This enzyme scavenges exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 11">
            <text>2'-deoxycytidine + H(+) + H2O = 2'-deoxyuridine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:13433"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15698"/>
            <dbReference type="ChEBI" id="CHEBI:16450"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>cytidine + H(+) + H2O = NH4(+) + uridine</text>
            <dbReference type="Rhea" id="RHEA:16069"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16704"/>
            <dbReference type="ChEBI" id="CHEBI:17562"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3 11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5 11">Belongs to the cytidine and deoxycytidylate deaminase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.5" evidence="6 11"/>
    <dbReference type="EMBL" id="CBAR010000208">
        <property type="protein sequence ID" value="CCZ84293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00032"/>
    <dbReference type="EnsemblBacteria" id="CCZ84293">
        <property type="protein sequence ID" value="CCZ84293"/>
        <property type="gene ID" value="BN566_00032"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004126">
        <property type="term" value="F:cytidine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047844">
        <property type="term" value="F:deoxycytidine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016192">
        <property type="entry name" value="APOBEC/CMP_deaminase_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006262">
        <property type="entry name" value="Cyt_deam_tetra"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="SSF53927"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01354">
        <property type="entry name" value="cyt_deam_tetra"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00903">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="12">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00003949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00018266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CCZ84293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="126" mass="13521" checksum="1C4EB8FA68B10BEA" modified="2013-07-24" version="1">MKTMTDYALEARERAYAPYSGFSVGAALQGTDGRMFLGCNVENASYSMTICAERNAVLQAIAAGQRSFRSIAIVGGKTAEEAQKTPCIPCGACLQVLAEFCDGDFPILLTDGVHLLRDFLPQAFTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5V190</accession>
    <name>R5V190_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CRISPR-associated exonuclease Cas4</fullName>
            <ecNumber evidence="6">3.1.12.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_00266</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="6">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="6">Mg(2+) or Mn(2+) required for ssDNA cleavage activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>iron-sulfur cluster</name>
            <dbReference type="ChEBI" id="CHEBI:30408"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the CRISPR-associated exonuclease Cas4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.12.1" evidence="6"/>
    <dbReference type="EMBL" id="CBAR010000218">
        <property type="protein sequence ID" value="CCZ84353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00266"/>
    <dbReference type="EnsemblBacteria" id="CCZ84353">
        <property type="protein sequence ID" value="CCZ84353"/>
        <property type="gene ID" value="BN566_00266"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013343">
        <property type="entry name" value="CRISPR-assoc_prot_Cas4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022765">
        <property type="entry name" value="Dna2/Cas4_DUF83"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01930">
        <property type="entry name" value="Cas_Cas4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00372">
        <property type="entry name" value="cas4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="6" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0408">Iron</keyword>
    <keyword evidence="3 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 6" id="KW-0464">Manganese</keyword>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cas_Cas4" evidence="7">
        <location>
            <begin position="12"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ84353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="224" mass="25736" checksum="421F5CE40BF05C2B" modified="2013-07-24" version="1">MRYSEDEYLMLSGIQHFCFCRRQWALIHLEQQWAENVRTVDGEIFHKNAHDKERHETRGNQIIVRGMPVSSATLGISGECDVVEFHRSETGVSIHGKVGTYDIIPIEYKRGEAKKEDCDRLQLAAQAMCLEEMLCCTIPIGYLYYGKTRRRSKVEIDESLREKTIAMLTEMHQLYRTQHTPKVRRTKACNACSLKQLCLPVLCGTQSAAAYMQAMLKPTEEVSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1F8</accession>
    <name>R5V1F8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01811</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000186">
        <property type="protein sequence ID" value="CCZ84085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84085">
        <property type="protein sequence ID" value="CCZ84085"/>
        <property type="gene ID" value="BN566_01811"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="140" mass="16654" checksum="C305667446AE7504" modified="2013-07-24" version="1">MYLIYILYAREKKSKIFFYIERYGNDLHRPTSFLFHRLEERSFSHTKQKHLQLLLYVNQHLAQAFSFIFVGFAPVPKATVTDCHEKVQKSPKTGKKRRQCRKGNTQKNFRFLGKVCKRPTSTDIRPPSKRCKKARKTGRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1V2</accession>
    <name>R5V1V2_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Methionine--tRNA ligase</fullName>
            <ecNumber evidence="13">6.1.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Methionyl-tRNA synthetase</fullName>
            <shortName evidence="13">MetRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">metG</name>
        <name evidence="17" type="ORF">BN566_00209</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 13">Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met)</text>
            <dbReference type="Rhea" id="RHEA:13481"/>
            <dbReference type="Rhea" id="RHEA-COMP:9667"/>
            <dbReference type="Rhea" id="RHEA-COMP:9698"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78530"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="13">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 13">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.10" evidence="13"/>
    <dbReference type="EMBL" id="CBAR010000207">
        <property type="protein sequence ID" value="CCZ84280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00209"/>
    <dbReference type="EnsemblBacteria" id="CCZ84280">
        <property type="protein sequence ID" value="CCZ84280"/>
        <property type="gene ID" value="BN566_00209"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004825">
        <property type="term" value="F:methionine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006431">
        <property type="term" value="P:methionyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07957">
        <property type="entry name" value="Anticodon_Ia_Met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00814">
        <property type="entry name" value="MetRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02800">
        <property type="entry name" value="tRNA_bind_EcMetRS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01228">
        <property type="entry name" value="Met_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041872">
        <property type="entry name" value="Anticodon_Met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004495">
        <property type="entry name" value="Met-tRNA-synth_bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014758">
        <property type="entry name" value="Met-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023457">
        <property type="entry name" value="Met-tRNA_synth_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015413">
        <property type="entry name" value="Methionyl/Leucyl_tRNA_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033911">
        <property type="entry name" value="MetRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002547">
        <property type="entry name" value="tRNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43326">
        <property type="entry name" value="PTHR43326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19303">
        <property type="entry name" value="Anticodon_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09334">
        <property type="entry name" value="tRNA-synt_1g"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01588">
        <property type="entry name" value="tRNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01041">
        <property type="entry name" value="TRNASYNTHMET"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00398">
        <property type="entry name" value="metG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00399">
        <property type="entry name" value="metG_C_term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50886">
        <property type="entry name" value="TRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 13 15" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="9 13 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 13 15" id="KW-0436">Ligase</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 13 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11 13 15" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 13 14" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 13 14" id="KW-0820">tRNA-binding</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="TRNA-binding" evidence="16">
        <location>
            <begin position="548"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="13">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="13">
        <location>
            <begin position="297"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CCZ84280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="649" mass="73194" checksum="10B995831166AF57" modified="2013-07-24" version="1">MAAKPYYITTPIYYPSGNPHIGHCYTTVACDSIARYRRMQGYDVMFLTGTDEHGLKIEQKAAEKGVTPKEYVDEIVKTFKSLWSYMNISYDRYIRTTDDYHVETVQKIFKELYDKGYIYKGKYQGKYCTPCESFWTESQLVDGKCPECGRPVIEAEEEAYFFKMSPFAERITHLLTETDYLQPKTRATELVNNFIKPGLEDLCVSRTSFTWGIPVTFDPKHIVYVWVDALSNYISALGFYNDAYHDYDRYWPADVHMVAKDIMRFHAIIWPAILMALDLPLPKHLAVHGWITFNGQKMSKSLGNVVDPFVLGERYGADAIRYHILREMALGSDSSFSNEVLITRINSDLANGLGNLVSRTVAMVDKYFNGTLPEDREAGEFDDDLIATATALREKVDGFMDQTQLNHALAEIFKVVSRANKYIDETAPWVLAKDESKKARLATVLYNLLDTIRIISTLLSAFMPTTMPKVWEQIGAGEAERTYENAASFDVLPKNVTVHKGEILFPRIDVEKEIEELNAIIKNNEPQQPAEEEAAPVAELAPEITIDDFAKVDLRIAKVVECEKVKKSKKLLKLQLDDGMGGRQVVSGIAQWYKPEDLIGKKIILVANLKPAKLCGVESNGMICAADAPDGSASVVFPDQSLPCGAKIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1W9</accession>
    <name>R5V1W9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6 8">50S ribosomal protein L16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rplP</name>
        <name evidence="10" type="ORF">BN566_00064</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 8">Binds 23S rRNA and is also seen to make contacts with the A and possibly P site tRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 8">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the universal ribosomal protein uL16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000241">
        <property type="protein sequence ID" value="CCZ84518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00064"/>
    <dbReference type="EnsemblBacteria" id="CCZ84518">
        <property type="protein sequence ID" value="CCZ84518"/>
        <property type="gene ID" value="BN566_00064"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01433">
        <property type="entry name" value="Ribosomal_L16_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01342">
        <property type="entry name" value="Ribosomal_L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016180">
        <property type="entry name" value="Ribosomal_L10e/L16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036920">
        <property type="entry name" value="Ribosomal_L10e/L16_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000114">
        <property type="entry name" value="Ribosomal_L16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020798">
        <property type="entry name" value="Ribosomal_L16_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12220">
        <property type="entry name" value="PTHR12220"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00252">
        <property type="entry name" value="Ribosomal_L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00060">
        <property type="entry name" value="RIBOSOMALL16"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54686">
        <property type="entry name" value="SSF54686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01164">
        <property type="entry name" value="rplP_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00586">
        <property type="entry name" value="RIBOSOMAL_L16_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00701">
        <property type="entry name" value="RIBOSOMAL_L16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 6 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2 6 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 8" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="6 8" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Ribosomal_L16" evidence="9">
        <location>
            <begin position="4"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ84518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="140" mass="15702" checksum="15C287D97F9E77AB" modified="2013-07-24" version="1">MLLPKRVKYRRVHRGRMTGKAYRGSTVSNGEYGLQALEPAWITSNQIESARIAMTRYIKRGGQVWIKIFPDKPVTKKPAGTRMGSGKGAPEYWVAVVKPGRVMFEIAGVPEETAREAMRLAMHKLPIKCKFVKAEGGEQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1Y0</accession>
    <name>R5V1Y0_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Methionine aminopeptidase</fullName>
            <shortName evidence="2">MAP</shortName>
            <shortName evidence="2">MetAP</shortName>
            <ecNumber evidence="2 3">3.4.11.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Peptidase M</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">map</name>
        <name evidence="5" type="ORF">BN566_00079</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 2">Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.</text>
            <dbReference type="EC" id="3.4.11.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="2 3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="2 3">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="2 3">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="2 3">Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe(2+)-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.18" evidence="2 3"/>
    <dbReference type="EMBL" id="CBAR010000241">
        <property type="protein sequence ID" value="CCZ84533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00079"/>
    <dbReference type="EnsemblBacteria" id="CCZ84533">
        <property type="protein sequence ID" value="CCZ84533"/>
        <property type="gene ID" value="BN566_00079"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070084">
        <property type="term" value="P:protein initiator methionine removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01086">
        <property type="entry name" value="MetAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01974">
        <property type="entry name" value="MetAP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001714">
        <property type="entry name" value="Pept_M24_MAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002467">
        <property type="entry name" value="Pept_M24A_MAP1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00599">
        <property type="entry name" value="MAPEPTIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="SSF55920"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00500">
        <property type="entry name" value="met_pdase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00680">
        <property type="entry name" value="MAP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 5" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 3" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_M24" evidence="4">
        <location>
            <begin position="11"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ84533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="249" mass="26501" checksum="BB198498837319BA" modified="2013-07-24" version="1">MITIKSRSDLEKLREAGRVAAQALELAGKSIHPGMTTHQLDKILHDFIVHSGATPTFLGYGGFPASACISINEEIIHGIPSRHRVIKAGDIVSVDVGATLNGFVGDNAATFPVGDVSQEALDLLAVTKASLFEAIAAAQVGARLGDVCHAVEAYCSERGYGIVREYCGHGIGREMHEDPEVPNYGKPGHGVRLMPGMTFCIEPMINQKGDAVRVLKDGWTVVTANGSLSAHFEHEIAVTKDGPVILTRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1Z1</accession>
    <name>R5V1Z1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transcriptional regulator with HTH domain and aminotransferase domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00243</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000214">
        <property type="protein sequence ID" value="CCZ84330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00243"/>
    <dbReference type="EnsemblBacteria" id="CCZ84330">
        <property type="protein sequence ID" value="CCZ84330"/>
        <property type="gene ID" value="BN566_00243"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="3">
        <location>
            <begin position="12"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="464" mass="52511" checksum="9DDE0D6457FD938D" modified="2013-07-24" version="1">MLTYSFTNIGSESLYQHLYQCIRNDVVSGILPPGTKMPSKRSFALHLGISVMTVESAYSQLVAEGYLYSLPRKGFYVADVRSQKPQIAADVALFVPEAAPVWFADFVSNHTEPDNFPFSIWNRLMRKCMTQHQQDLMQNAPAGGRSELRQAIANHLSAFRGMQVAPEQIIIGAGTDFLYGLLIQLLGYDRHYAAEDPGYAKIAKIYRSHQVAFTPVPIDQSGVSVEALQACGADVVHCSPSHHYPTGIVMPVSRRYTLLGWATEQKNRYIIEDDFDSEFRMTGKPLPTLQSMDTTGRVIYMNTFTKSLSSTIRIGYMVLPPPLTERFYEKLGFYACTVSGFEQLVLAHFLAEGYFETHINRMRNRYRQKRDTLISCLRAAMPPEMTEILEEEAGLHFLLRIQTQADDAELVQRVERQGLHVACLSSYYAQQEQAEAHTLVLNYSNVPEEKMQEAAERLQRAIFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5V255</accession>
    <name>R5V255_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00307</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000224">
        <property type="protein sequence ID" value="CCZ84400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84400">
        <property type="protein sequence ID" value="CCZ84400"/>
        <property type="gene ID" value="BN566_00307"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004406649" evidence="1">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="52" mass="5468" checksum="BEB9AF4CF42DEB66" modified="2013-07-24" version="1">MMKLTTTRSIAALVAALLLSITCLTIGSMALTATQTDTPMITASIQEEETPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>R5V256</accession>
    <name>R5V256_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">LPXTG-motif cell wall anchor domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00471</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000257">
        <property type="protein sequence ID" value="CCZ84618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84618">
        <property type="protein sequence ID" value="CCZ84618"/>
        <property type="gene ID" value="BN566_00471"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00222">
        <property type="entry name" value="CollagenBindB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008966">
        <property type="entry name" value="Adhesion_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003343">
        <property type="entry name" value="Big_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008454">
        <property type="entry name" value="Collagen-bd_Cna-like_B-typ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008964">
        <property type="entry name" value="Invasin/intimin_cell_adhesion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013830">
        <property type="entry name" value="SGNH_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032789">
        <property type="entry name" value="T2SS-T3SS_pil_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02368">
        <property type="entry name" value="Big_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05738">
        <property type="entry name" value="Cna_B"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13472">
        <property type="entry name" value="Lipase_GDSL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13629">
        <property type="entry name" value="T2SS-T3SS_pil_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00635">
        <property type="entry name" value="BID_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49373">
        <property type="entry name" value="SSF49373"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49401">
        <property type="entry name" value="SSF49401"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0572">Peptidoglycan-anchor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="3533"/>
            <end position="3552"/>
        </location>
    </feature>
    <feature type="domain" description="BIG2" evidence="3">
        <location>
            <begin position="2821"/>
            <end position="2905"/>
        </location>
    </feature>
    <feature type="domain" description="BIG2" evidence="3">
        <location>
            <begin position="2910"/>
            <end position="2984"/>
        </location>
    </feature>
    <feature type="domain" description="BIG2" evidence="3">
        <location>
            <begin position="2987"/>
            <end position="3060"/>
        </location>
    </feature>
    <feature type="domain" description="BIG2" evidence="3">
        <location>
            <begin position="3165"/>
            <end position="3236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="3562" mass="390447" checksum="CA7A29589767725A" modified="2013-07-24" version="1">MNINQKIQEFLTEHQYQKKRRIFTAVLSLMIVFSVVSSLIMPAISMTMQDLDDAAAVDTIDAEPAEENMMLLGLGDENRQTEPINLAERATSSGGSFNISAIDIGEDGKGKNEIDNNYVVSTDKTNIKFEVSYTLSNMKDVFKKDADFEHLYIDIENFAINNTYNGILNDEAYSDYMAKNGHGIVNPGTYKVEENRIKLYLTDDYIKYIDGGEGNVTGTLNFSGELSRNNTASGDQTIKIGGKDIVIPFQDKQAGVEKNYWVDSSKGEIEWTITVKPNGLSLKDYTLVDNMLQKASGDVFINPSSAATYNPNDKKVTFDESNTGDVTIKYRTKIGTADLQAGNVTNKATLQKDGENPIEDSKTVTFDKTPVNVTKDGQADYEKGKSRNNKIDWTITIASKYGTSLNGYQIKDANLPDNDVTISPSGTLTKNGDGTWTLNVADNVTGVTLNYSANATDGDNTNSVSIHYPDGSPTDGKAEKTVYYKKESEMISVNKNGNYNQDTHEITWTIQVTPVNGYSLKDYYLEDSQFPSSIDQFTASGCNTSDFTISGNRLTFTSNIKQAVTLQYKTKVSVPDNDTNAEATTVVTNKIEDKFTTTKVVSVPVKSRNTITKTVVGDSYVPEPTSNAISQEFSWKVDITRDGSFDGYIYQDTLTASTNGTHTITADQLAALKVFAKKEYNGNAIELKQGTDYTVDRKTDGSGFEVKFLSTTKDYNYISFKYSTTATIPESAAYGQYIFNNKGSSNKGGGEPNPGVTIEKKNPVQTIDMTVRKDWANDNANVRPNSITFKVQYQENNTGDWKDLKQSGNTYLFEGDNDYSSASVVEVTVDSNGNWATTVSNLPVSVTKNNTEKTYQYRVQEIKYNDTAIKNGEISINSGIYRANNNGISIAVSQNNRTAVVTNTYYPNISLTPVKDWKDSNNQTIPNYNGDITEITVQLVSKNSDGKFYPVKDSSGSPLTAKLNASNGWGKDLTAWSGLSSEKEYRLIETEVKIGNDTKPVFTVPDNGDYYSNKETSFVVGDTYYKAALAENVTKVSSDTNISVTNTVYEKKNLQIGVTKQWSPSKPDGVSGVVVELQRKASNSNSWTAYPENDTTKSQKTLNDSNNWHASWSELPNQNVDNTGRISYTYRVVEVGYVKADGTTVVSIQNDKFALANAQGNADGLYEASYVNQELTKDGTVQITNTYKQLTSIELTPEKKWEGDIDSQTQNPFVERPKSITLQLQQKLGENGTWVSMEGKTLTLTKNDQSQYDKSTWKSDSKKFENLPEKVIRVNADGSYTEQKYYYQLVEIGYTPNGSDTAISIPAGETSFEVTAQNNGQTYNGRYSFSSDVNNGYSGSLKIKNTYKEDIGLSKNIVIGRTSSNSISISKDELTQFKKKIGTEDYYIFNYTVDFSSSQKDAASPFSDIIPEGFEFCENSNWDGIQMAWQSGSTIDQYSPLTGDPGNIAKHFDGYYEHPVFVWPTYGINSAKVASDLNTIWSQFGKGEWYYYDRANDRVYFNKPDLWAKMYICYSIKIKCADLEAKIANGNYEILNQVIKHEKDGAETAQKDSASVIIKNQTPTDLITKTYQSAALPGYISYTLDINPQGKTLSSGDTIDIEDLFKTVSYYDSDWNGGETTNGENLVDVLMRNINIYKIDANGDKQRLSASEYTLQFDCSENGVQSDGEKGAALLKLTVPDATHLQVTYSYKIIANKNTPSVIHGCKVRKNGRYVPMESGLVPPAGDKITFSNTAKLKADSASGESSHNNQEYTVFQSGGTISTNPIPKIYKVNTGDYTIKSLHASFLLAKYESGNWYYASNVNADGAITWGKQSFNGKNVPATDAYVIKVEGTQPKISLEQNVLYKLVEISVPAGYEGSNLNLSSTDFRALVTGYLNSNLTTYNNKDYATFLNHYNPNHYFSFNSNVSGNHIPHDVSQSEIQQIKSGDDLNIPNSELIDIGVNKQWVNSTNTIPENASITVELYWSYEKSTSGIPASATLAKAEDLGILDSSFTATKTLTKPENAKVWTDLPNGKAGKPIYYYVKETAYTYGSNTYTLQEGGSYKSGSDLGGYLPIYQNNAANGDATVQIQNTQRLMLKKMWKDINNEDMNPLSSSVNVTVYGVKVDSAGNETKEALFTNPVTLGDTNSWQLDITSLIGNKDLSVYKRFEVTETGVDTSNMVISCVFNLNQNTGEIIVTNKSTQPTDASVTVQKAWSDGETLHASESVQVSLYQSTTALPSGMTLDANWITANATKMTDTETATYTVQLNKDNEWTYTWTGLPLENATKQPYYYYVLEDLQNSTVANKDKYTATYTKSSNSTAYKTNYTITNSRSAITVQKQWYDEDGNLITNLYDEDGNLIVNNMANLQEITLKVYKKTGTVPTDPIGIVAFGDSITDGYNNSWETGGLNCSKNEKCYPSKLTTMLTAAGFKLKNDAVANKGNSGEQIGEAGNGFRSRVTSDIPADTKIVCLLGGTNDIHQGGSSVKGDPDGVFNRLQGLISEIKTQAPDATIFVGSIPHFDFYKNETLTTGGNWWNWLSGYAVNDGAKPNGLIDEYNAKIKAYAEETAGVYFVDVCSVVTDDDIRADGCHPNETGYTKIATAYSNAIQDYYTNKEPVQENGQDLTITLNNSNNWRAAIDVPAGNDTYCVEEVNVPDGWDVTYENNAQQANRTTPILVKNQKQPTDIDLTVEKTWAKDDASNRPDSISLTLLRSNRKKQDNSDAANTSEWFWEELRISTPTPTKNGNKWTFAYTGLPASDAFGNAYYYKVQEAAVSGYTVSYGLNGAGEENGVTAAAGETATLHVTNTRAITLQIEKQWSDGATNQHLKDAVRVRIYRSTDQTKVPTANLTLQVTPETVSVGVNGTATVTANKKITVKEIANDTIANATISEDGKTLTITGEGAGETTITVTDGTMEKRISVIVSVEPTLNLDIEPKSIQVGETAILTPSMSDGSDCSGVTYSITKGTDVVSISGNTVTGSKAGTATIVAERNGKTSKPVTITVTEPPLSLDKDNVTVSVGDTATIQANRTVTLSQNPDASIATASVSGKNITVTGVAAGSTSFTVKDSDGQEKTVLVTVEKSVEFKLYKDGVDVTNKGLSYDDRFKVKNGTVVTFKTSIPFTNVETTDGWFISVNKVDEKTFTVGVGQYKNPSAYDNGFNITYNDASGTSITKKYFVEITDADPPITLTASSKFVKTEKTLQIKSNVTGVTYSSSNAQIATVDATTGLVTGVSVGEVTITAKKNGYTDGTINLTVTDVDITGKVLTSNEVYTFNIPEKYQDNIKKLEVSFADYSATNNAGINVYFNASNAIDTQPNSWIEFDGSNGNMKNLYIFNDHNNYFSKVNYQFGIVNGNTAIWEKNSASKNEKIIFQAKDTVNCTITKISIINEAGETHTITNFEETYTPQSLSAPRRAVAAAAVIESEQLLSATNETAGVQTQALENTIDASEVSDSDWASGLLLEIDATDHWQGSVTNLPVTDSNGNTYYYWAVEEPVTGYTPSYLFQDADGSQSNAIKADAQADGTDIIVLNTKQDTSYTLPSTGGTGVTRYYLIGLLLMGGSGLVVCYQFRRKRHGNCAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5V2J6</accession>
    <name>R5V2J6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Sporulation protein YabP</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00399</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000240">
        <property type="protein sequence ID" value="CCZ84500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00399"/>
    <dbReference type="EnsemblBacteria" id="CCZ84500">
        <property type="protein sequence ID" value="CCZ84500"/>
        <property type="gene ID" value="BN566_00399"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022476">
        <property type="entry name" value="Spore_YabP/YqfC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038705">
        <property type="entry name" value="YabP_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07873">
        <property type="entry name" value="YabP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="96" mass="10708" checksum="0D009A220258D73A" modified="2013-07-24" version="1">MKTDRSAVSANLHHNLILEDRSAMNLSGVTDVDCFDERTIRLYTQLGELVIKGKNLHIDTVSVETGDMRVSGDIWSLQYGDKDRKGALSALGKLFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>R5V2M1</accession>
    <name>R5V2M1_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">30S ribosomal protein S19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpsS</name>
        <name evidence="8" type="ORF">BN566_00061</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6 7">Belongs to the universal ribosomal protein uS19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000241">
        <property type="protein sequence ID" value="CCZ84515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00061"/>
    <dbReference type="EnsemblBacteria" id="CCZ84515">
        <property type="protein sequence ID" value="CCZ84515"/>
        <property type="gene ID" value="BN566_00061"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.860.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00531">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020934">
        <property type="entry name" value="Ribosomal_S15/S19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002222">
        <property type="entry name" value="Ribosomal_S19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005732">
        <property type="entry name" value="Ribosomal_S19_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023575">
        <property type="entry name" value="Ribosomal_S19_S15_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880">
        <property type="entry name" value="PTHR11880"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00203">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002144">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00975">
        <property type="entry name" value="RIBOSOMALS19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54570">
        <property type="entry name" value="SSF54570"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01050">
        <property type="entry name" value="rpsS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00323">
        <property type="entry name" value="RIBOSOMAL_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ84515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="94" mass="10483" checksum="8038BCA896CDAD29" modified="2013-07-24" version="1">MSRSIKKGPFVQEVLLKRVQAMNEAGEKKVLKTWSRSSTIFPDFVGHTFAVHDGRKHVPVYVTEDMVGHKLGEFAPTRTFKGHAGSKTSNNGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>R5V2X8</accession>
    <name>R5V2X8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 8">Methionyl-tRNA formyltransferase</fullName>
            <ecNumber evidence="4 8">2.1.2.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">fmt</name>
        <name evidence="11" type="ORF">BN566_00483</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 8">Attaches a formyl group to the free amino group of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by promoting its recognition by IF2 and preventing the misappropriation of this tRNA by the elongation apparatus.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>(6S)-10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) = (6S)-5,6,7,8-tetrahydrofolate + H(+) + N-formyl-L-methionyl-tRNA(fMet)</text>
            <dbReference type="Rhea" id="RHEA:24380"/>
            <dbReference type="Rhea" id="RHEA-COMP:9952"/>
            <dbReference type="Rhea" id="RHEA-COMP:9953"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57454"/>
            <dbReference type="ChEBI" id="CHEBI:78530"/>
            <dbReference type="ChEBI" id="CHEBI:78844"/>
            <dbReference type="EC" id="2.1.2.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the Fmt family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.9" evidence="4 8"/>
    <dbReference type="EMBL" id="CBAR010000257">
        <property type="protein sequence ID" value="CCZ84630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00483"/>
    <dbReference type="EnsemblBacteria" id="CCZ84630">
        <property type="protein sequence ID" value="CCZ84630"/>
        <property type="gene ID" value="BN566_00483"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004479">
        <property type="term" value="F:methionyl-tRNA formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd08646">
        <property type="entry name" value="FMT_core_Met-tRNA-FMT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08704">
        <property type="entry name" value="Met_tRNA_FMT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00182">
        <property type="entry name" value="Formyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005794">
        <property type="entry name" value="Fmt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005793">
        <property type="entry name" value="Formyl_trans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002376">
        <property type="entry name" value="Formyl_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011034">
        <property type="entry name" value="Formyl_transferase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044135">
        <property type="entry name" value="Met-tRNA-FMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041711">
        <property type="entry name" value="Met-tRNA-FMT_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02911">
        <property type="entry name" value="Formyl_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00551">
        <property type="entry name" value="Formyl_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50486">
        <property type="entry name" value="SSF50486"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="SSF53328"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00460">
        <property type="entry name" value="fmt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Formyl_trans_N" evidence="9">
        <location>
            <begin position="1"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Formyl_trans_C" evidence="10">
        <location>
            <begin position="207"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Tetrahydrofolate (THF) binding" evidence="8">
        <location>
            <begin position="113"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ84630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="315" mass="33899" checksum="BAB12B43B1DC918C" modified="2013-07-24" version="1">MRIVFMGTPDFAVPCLQALLQQAYEVVGVFTQPDKPKGRGYKMIPPPVKAAAVEAGLPVYQPLSLRKGEEAEQAMQTLQELNPDVIVVVAYGQILPKAVLDLPKYGCINIHGSLLPAYRGAAPMQMCLLNGETETGVTSMQMAEGLDTGDMLVKAALPIGADETFASLHDRLAELGASVLLETLEKLEAGTLQPEPQDDAKSSYAGRITKEMSALHFEQPANTLHNIIRGITGFTTLDGKRLKVYASHVVTTAMPEELPYGAVADAEKLIVKCGDGLGLQLLEVQPEGKKRMKAVDFLRGKSIPYGTVLGKVSQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5V305</accession>
    <name>R5V305_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phage shock protein A (IM30) suppresses sigma54-dependent transcription</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00513</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PspA/IM30 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000267">
        <property type="protein sequence ID" value="CCZ84660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00513"/>
    <dbReference type="EnsemblBacteria" id="CCZ84660">
        <property type="protein sequence ID" value="CCZ84660"/>
        <property type="gene ID" value="BN566_00513"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007157">
        <property type="entry name" value="PspA_IM30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31088">
        <property type="entry name" value="PTHR31088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04012">
        <property type="entry name" value="PspA_IM30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="115"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="241" mass="26396" checksum="46D42D4DE5B2A3C0" modified="2013-07-24" version="1">MGIFSRIGDIMKANVNDLIDKAEDPEKMVKQIIRDLQQEVAKSTQALGKAMASQRIAERQYKTALQSSADWESKAKAALAAGNTEMAKKALSYKVKADNDVASYQQMYETITTQTDAIRNQVETLKAKLEEAKSRQAMLIARSQMAKTQKNLAQSMGGINTSSSMDKFNRMEEKIMQQEAEAAAFAEISGNSNADTKSFEQMEADTKVDAELQRLMAEMGTSTSDADLDAQLAALEQGGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>R5V506</accession>
    <name>R5V506_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Non-specific protein-tyrosine kinase</fullName>
            <ecNumber evidence="6">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">BN566_00987</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-tyrosyl-[protein] = ADP + H(+) + O-phospho-L-tyrosyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:10137"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:82620"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell inner membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CpsD/CapB family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the etk/wzc family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="6"/>
    <dbReference type="EMBL" id="CBAR010000070">
        <property type="protein sequence ID" value="CCZ83204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00987"/>
    <dbReference type="EnsemblBacteria" id="CCZ83204">
        <property type="protein sequence ID" value="CCZ83204"/>
        <property type="gene ID" value="BN566_00987"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045226">
        <property type="term" value="P:extracellular polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009103">
        <property type="term" value="P:lipopolysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025669">
        <property type="entry name" value="AAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005702">
        <property type="entry name" value="EPS_synthesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003856">
        <property type="entry name" value="LPS_length_determ_N_term"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13614">
        <property type="entry name" value="AAA_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02706">
        <property type="entry name" value="Wzz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01007">
        <property type="entry name" value="eps_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="15 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="16" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="205"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Wzz" evidence="19">
        <location>
            <begin position="17"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="AAA_31" evidence="20">
        <location>
            <begin position="306"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="500"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF13614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="CCZ83204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="534" mass="58231" checksum="41A7467A0A10FC71" modified="2013-07-24" version="1">MGTLNQKNKNKQDDETEIDLGKLFKALMQRLPIILLVTLCFSAIGFSYGKYYLPLEYTSSTYMYVKNGDTSEEKTAINQQDLMASKSLVETYIVILKNDTVVKQVGMSLVSKFGVSEISKCFTVKNGVISTSELKDAISMSADGDTEVLKISAQTKDPHVSAEICDIYAEVAPTFLIRIIGAGSVEVIGDAEIPQSPSAPDVKKITMMAAAAGFVLSAGVAVLIYLLDRTIKGEDDIQSFSISYLGEVPEMVEARSGKRKRRKKKNSPSALRNMLLCAKDIPFPCSEAYRSIRTNLMFALAPQEHKIVAVTSPNAADGKSLTAANIAMAMAMMNKKVLFIDADLRKPVQHQRLKLENKVGLSEVLGHMTTWQVARHLAPLPNMDVLTAGTCPPNPSELLASPAMKKLLEEAEQQYDYIIIDTPPVNVVSDALGLSDCIGGILMVARYYVTTTKELETALQAISVTGATVLGLVLTEVDFKQGGYYKKYYKKGSYYTYGNGNGNGSKKKSHSHSTQQSEPVSTTTDTDSDKQPVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>R5V552</accession>
    <name>R5V552_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Na_H_Exchanger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_01035</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01035"/>
    <dbReference type="EnsemblBacteria" id="CCZ83254">
        <property type="protein sequence ID" value="CCZ83254"/>
        <property type="gene ID" value="BN566_01035"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015299">
        <property type="term" value="F:solute:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006812">
        <property type="term" value="P:cation transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="41"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="94"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="130"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="164"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="207"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="249"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="297"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="323"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="391"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="Na_H_Exchanger" evidence="5">
        <location>
            <begin position="20"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ83254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="435" mass="46791" checksum="1FDC1D92B9B78805" modified="2013-07-24" version="1">MAGIKDFLFDNSYAYLLQIAIILLATKLLGLVTKRISLPQVVGALAAGIILGPMLLDVVERNSLISNLAELGVIVLMFSAGLETDLQELKKAGLASFVIALLGVLVPLGGGFFLAHFFNTEQDPEMSSLFWQNMFIGVIMTATSVSITVETLKELGVLNSRAGNAILGAAVIDDILGIIALTVIISLGGKTGEEGGEKTFGSFAGDGVLTVFINILAFFIFAIAAAVIYHKWFKKWREASATNLRRHIIVTFAFCLILSFCAEVFFGVADITGSFVAGLAVSGLRRNEYVVSRFNTLSYMLLSPIFFANIGLAMTLKGLNGKLVLFMVLLMIVALISKVIGCGLGAKIMRYTNKESLQIGIGMISRGEVALIVASKGAAVGLMSEKYMTPIVIMVVFTTIITPILLKFVFPKQNTPLSPEQQERIQKKELADAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5V5B4</accession>
    <name>R5V5B4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01105</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000084">
        <property type="protein sequence ID" value="CCZ83324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83324">
        <property type="protein sequence ID" value="CCZ83324"/>
        <property type="gene ID" value="BN566_01105"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="73" mass="8177" checksum="9677A08F2ABA30EE" modified="2013-07-24" version="1">MRNVRGASSGDFNPLPRKEGDSQFKSFLDCEAHFNPLPRKEGDNHSGKRNCDARHFNPLPRKEGDLPVTALCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>R5V6Y9</accession>
    <name>R5V6Y9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cadmium-exporting ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_01598</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000169">
        <property type="protein sequence ID" value="CCZ83869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01598"/>
    <dbReference type="EnsemblBacteria" id="CCZ83869">
        <property type="protein sequence ID" value="CCZ83869"/>
        <property type="gene ID" value="BN566_01598"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00941">
        <property type="entry name" value="CDATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="SSF81653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="SSF81665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-1278">Translocase</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="237"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="276"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="580"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="605"/>
            <end position="623"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ83869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="626" mass="67118" checksum="33D13379AF4195FE" modified="2013-07-24" version="1">MTRKQKRMLIRILIAAVLFLAALITDHTLHPSMWIVLPIFAAAYLVVGWDILWKAIRNISHGQIFDENFLMAIASLGAFAMQEFDESVAVMLLYQIGELFQSYAVNKSRNAISSLVELRPDVATVERNGELLEVDPDEVAVSETIVVKTGERIPLDGIILEGTSTLDTAALTGESLPRSVTVGDEALSGCINQSGLLKIRVTKPFEQSTVSKILELVEEASERKSKSEAFITRFAKYYTPFVVIAAVLLSILPPILTGNLLNGAVWLKWVSRALTFLVISCPCALVISVPLSFFGGIGGASKNGILIKGSNYLEALADVNTIAFDKTGTLTQGTFTVTGQYPENGTTEAQLLEWAAMAEAFSTHPIASSLREAYGKPIAKEQIQDVQEVAGNGVQATIQGHLVLAGKAAMLEQQNISVPAETKSLTGTKVFVAVDHVYQGCILIADPLKPTSAAAIQAIKALGVEKTVLLSGDTEQTAKAVANTLHMDEVHAQLLPQDKVQCLEDLLHSQKEGKLLAYVGDGINDAPVLTRADVGIAMGAMGSDAAIEAADVVLMDDNPDKLPVAIRIARKTKRIVRENIVFALGVKGIVLILGALGLANMWAAVFADVGVAMIAICNAIRTLRIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>R5V6Z5</accession>
    <name>R5V6Z5_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein translocase subunit SecE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">secE</name>
        <name evidence="9" type="ORF">BN566_01603</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Essential subunit of the Sec protein translocation channel SecYEG. Clamps together the 2 halves of SecY. May contact the channel plug during translocation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the Sec protein translocase complex. Heterotrimer consisting of SecY, SecE and SecG subunits. The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. Interacts with the ribosome. Interacts with SecDF, and other proteins may be involved. Interacts with SecA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the SecE/SEC61-gamma family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000169">
        <property type="protein sequence ID" value="CCZ83874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01603"/>
    <dbReference type="EnsemblBacteria" id="CCZ83874">
        <property type="protein sequence ID" value="CCZ83874"/>
        <property type="gene ID" value="BN566_01603"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043952">
        <property type="term" value="P:protein transport by the Sec complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00422">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005807">
        <property type="entry name" value="SecE_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038379">
        <property type="entry name" value="SecE_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001901">
        <property type="entry name" value="Translocase_SecE/Sec61-g"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33910">
        <property type="entry name" value="PTHR33910"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00584">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00964">
        <property type="entry name" value="secE_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0811">Translocation</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="70"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ83874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="102" mass="11449" checksum="2C88A07D8B68DAD8" modified="2013-07-24" version="1">MSDKEKKAALSEEKATKKEKKANAADSGKSKKEKEKKSVFKRIAAWFKDLKKEFKKVVWPTKKSVFNNTLVVLCVVIVGSVVIGLLDLGFLNLMQFLMGLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7G1</accession>
    <name>R5V7G1_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_01738</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CBAR010000178">
        <property type="protein sequence ID" value="CCZ84009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01738"/>
    <dbReference type="EnsemblBacteria" id="CCZ84009">
        <property type="protein sequence ID" value="CCZ84009"/>
        <property type="gene ID" value="BN566_01738"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="7">
        <location>
            <begin position="124"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ84009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="336" mass="38398" checksum="12230D17B5AD7EC5" modified="2013-07-24" version="1">MKQTNRILSITLEILFPLLCFGSVFFLTQNLLAAGIILVLSACQSFFLEWQTRKEQAYWSNIAAQLSDLCDCLLSLKHREIFSRNEDTIFSKLQDKVLKLVTILQQKNEDSLQAQENMKGLISDLSHQLKTPIASLKLYTALLEEPTLTEQQQKQYTEVLRTAIDRLIFLSESMIQLSRLESGLIQLQLEEKSLNQTVLMAIKNVFAKAKQHHITLTYDSEKELSLMHDQRWTAEAIFNLLDNAVKYSPPGSTVAIRVRELGLFVAVDVTDQAEPIPEDERSRIFQRFYRGQNSKATEGIGIGLYLARKIATEQNGHLNLRCRSNGNTFSILLFQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7K4</accession>
    <name>R5V7K4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Genome sequencing data contig C320</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01800</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000186">
        <property type="protein sequence ID" value="CCZ84074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84074">
        <property type="protein sequence ID" value="CCZ84074"/>
        <property type="gene ID" value="BN566_01800"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="73" mass="8736" checksum="B5D55B38C469E5D5" modified="2013-07-24" version="1">MTKVELERKLKDMNIKETMYDLNGGLPNEAYCMEEIGGKWYTYYSERGHRTSLKAFETEQEACSYFLDWIKNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7W4</accession>
    <name>R5V7W4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Bifunctional chorismate mutase/prephenate dehydratase</fullName>
            <ecNumber evidence="7">4.2.1.51</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Chorismate mutase-prephenate dehydratase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">p-protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">BN566_01939</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the Claisen rearrangement of chorismate to prephenate and the decarboxylation/dehydration of prephenate to phenylpyruvate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>H(+) + prephenate = 3-phenylpyruvate + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:21648"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:18005"/>
            <dbReference type="ChEBI" id="CHEBI:29934"/>
            <dbReference type="EC" id="4.2.1.51"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>chorismate = prephenate</text>
            <dbReference type="Rhea" id="RHEA:13897"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29934"/>
            <dbReference type="EC" id="5.4.99.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis; L-phenylalanine biosynthesis; phenylpyruvate from prephenate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Metabolic intermediate biosynthesis; prephenate biosynthesis; prephenate from chorismate: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.51" evidence="7"/>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01939"/>
    <dbReference type="EnsemblBacteria" id="CCZ84214">
        <property type="protein sequence ID" value="CCZ84214"/>
        <property type="gene ID" value="BN566_01939"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00120">
        <property type="reaction ID" value="UER00203"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00121">
        <property type="reaction ID" value="UER00345"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004106">
        <property type="term" value="F:chorismate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004664">
        <property type="term" value="F:prephenate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046417">
        <property type="term" value="P:chorismate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009094">
        <property type="term" value="P:L-phenylalanine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.59.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008242">
        <property type="entry name" value="Chor_mutase/pphenate_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036263">
        <property type="entry name" value="Chorismate_II_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036979">
        <property type="entry name" value="CM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002701">
        <property type="entry name" value="CM_II_prokaryot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001086">
        <property type="entry name" value="Preph_deHydtase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01817">
        <property type="entry name" value="CM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00800">
        <property type="entry name" value="PDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001500">
        <property type="entry name" value="Chor_mut_pdt_Ppr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00830">
        <property type="entry name" value="CM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48600">
        <property type="entry name" value="SSF48600"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51168">
        <property type="entry name" value="CHORISMATE_MUT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51171">
        <property type="entry name" value="PREPHENATE_DEHYDR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="14" id="KW-0456">Lyase</keyword>
    <keyword evidence="15" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="12" id="KW-0584">Phenylalanine biosynthesis</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chorismate mutase" evidence="19">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="Prephenate dehydratase" evidence="20">
        <location>
            <begin position="105"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="21">
        <location>
            <begin position="291"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="site" description="Essential for prephenate dehydratase activity" evidence="18">
        <location>
            <position position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00014401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00031175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00031520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR001500-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="CCZ84214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="372" mass="41584" checksum="4DF375B224EB53A1" modified="2013-07-24" version="1">MNLEEIRTEIDALDKELLALFERRMDLCRDVALYKKEHNLPVFQPEREEKLLDKIEQAASPDRKHAARTLFSVILDISKQLQSRILAKMEIDPIFPVPNFSAAQKIGCQGTNGANSETASQQFFPGRAVTFYPTFEHVFQAVERGEVEFGVLPVYNSTSGSVTPTYDLLGKYNCYITAMNQVKIKHCLAVRPGVSLEQIQAVYSHPQALLQCSDFLKYHNLKQIEYSNTATAAAFVASSSEPFAAICSEECAKQNHMDILFSGIANVALNYTRFICISKQMMVAPDASVISIMMTLPNVPGSLSKILNQFYLQGLDLVKLESRPIQDGSFDVAFYVDFKGNLMNRSIAAFLNELCKNCQDFKLLGNAVEVSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5V8G8</accession>
    <name>R5V8G8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 10">Serine acetyltransferase</fullName>
            <ecNumber evidence="4 10">2.3.1.30</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN566_00316</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>acetyl-CoA + L-serine = CoA + O-acetyl-L-serine</text>
            <dbReference type="Rhea" id="RHEA:24560"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:58340"/>
            <dbReference type="EC" id="2.3.1.30"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.30" evidence="4 10"/>
    <dbReference type="EMBL" id="CBAR010000224">
        <property type="protein sequence ID" value="CCZ84409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00316"/>
    <dbReference type="EnsemblBacteria" id="CCZ84409">
        <property type="protein sequence ID" value="CCZ84409"/>
        <property type="gene ID" value="BN566_00316"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00136">
        <property type="reaction ID" value="UER00199"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009001">
        <property type="term" value="F:serine O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03354">
        <property type="entry name" value="LbH_SAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045304">
        <property type="entry name" value="LbH_SAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042122">
        <property type="entry name" value="Ser_AcTrfase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005881">
        <property type="entry name" value="Ser_O-AcTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000441">
        <property type="entry name" value="CysE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01172">
        <property type="entry name" value="cysE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 10 11" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ84409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="217" mass="23466" checksum="96D16E4EDA25B9FA" modified="2013-07-24" version="1">MFRTLKEDIAIVKQKDPAARSTLEILLTYSGLKAVRSYRKAHWFYEHGFFTIARIISQRARHRTGIEIHPGAKIGKGLFIDHGMGVVIGETTEIGENCLLYQGVTLGGTGKDKGKRHPTLGDNVMVGAGAKVLGPITIGNNVKVAANAVVLKPIPDNCTAVGVPARVARCAGKKIVEDLDQMTCPDLNLQDIDALKSSVTALHQELSALEDQKTEDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>R5V8R1</accession>
    <name>R5V8R1_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">3-oxoacyl-[acyl-carrier-protein] synthase 2</fullName>
            <ecNumber evidence="3 12">2.3.1.179</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">BN566_00388</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Involved in the type II fatty acid elongation cycle. Catalyzes the elongation of a wide range of acyl-ACP by the addition of two carbons from malonyl-ACP to an acyl acceptor. Can efficiently catalyze the conversion of palmitoleoyl-ACP (cis-hexadec-9-enoyl-ACP) to cis-vaccenoyl-ACP (cis-octadec-11-enoyl-ACP), an essential step in the thermal regulation of fatty acid composition.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 12">
            <text>(9Z)-hexadecenoyl-[ACP] + H(+) + malonyl-[ACP] = 3-oxo-(11Z)-octadecenoyl-[ACP] + CO2 + holo-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:55040"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10800"/>
            <dbReference type="Rhea" id="RHEA-COMP:14074"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:83989"/>
            <dbReference type="ChEBI" id="CHEBI:138538"/>
            <dbReference type="EC" id="2.3.1.179"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>a fatty acyl-[ACP] + H(+) + malonyl-[ACP] = a 3-oxoacyl-[ACP] + CO2 + holo-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:22836"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:14125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:138651"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 12">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12 14">Belongs to the thiolase-like superfamily. Beta-ketoacyl-ACP synthases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.179" evidence="3 12"/>
    <dbReference type="EMBL" id="CBAR010000240">
        <property type="protein sequence ID" value="CCZ84489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00388"/>
    <dbReference type="EnsemblBacteria" id="CCZ84489">
        <property type="protein sequence ID" value="CCZ84489"/>
        <property type="gene ID" value="BN566_00388"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00834">
        <property type="entry name" value="KAS_I_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017568">
        <property type="entry name" value="3-oxoacyl-ACP_synth-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000794">
        <property type="entry name" value="Beta-ketoacyl_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11712">
        <property type="entry name" value="PTHR11712"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000447">
        <property type="entry name" value="KAS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03150">
        <property type="entry name" value="fabF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="B_KETOACYL_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8 12" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5 12" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PKS_KS" evidence="15">
        <location>
            <begin position="4"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR000447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000447-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CCZ84489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="412" mass="44070" checksum="B9751494F5819E64" modified="2013-07-24" version="1">MKRVVITGMGTVNPLGCNLETFWNNVKQGKLGISTIDTFDTTEVEISVAGIVRDFDPAAHLDKKDIRHMERFTQFAVVAAKEALEDCGSDLKDLDPYRCGVIIGCGVGGLEMTQKQHSIYLEKGPNRISPFFVPMMISNMAGGQVAMKTNFRGDNYCTVTACASATHAIGEAFRKIKDGYLDACLCGGTESSVDEFTIGGFKTMKALTKETDPAKASTPFDKNRSGFVLGEGCGVLLLEEYEHAVARGAKIYCELAGYGATCDAYHMTSPAPEGEAAAKAMVNAYTEAGLQPSDITYINAHGTSTGLNDKYETRAIKIALGEEAAKTVKINSTKSMTGHMLGGTGAVEAIVSAMSVHENFLHQTIGYTTPDPECDLDYCTEAPVECEVKAALSNSLGFGGHNATLCFKKCVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>R5V915</accession>
    <name>R5V915_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA gyrase subunit A</fullName>
            <ecNumber evidence="8">5.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">gyrA</name>
        <name evidence="11" type="ORF">BN566_00450</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterotetramer, composed of two GyrA and two GyrB chains. In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="8">Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the topoisomerase GyrA/ParC subunit family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the type II topoisomerase GyrA/ParC subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="8"/>
    <dbReference type="EMBL" id="CBAR010000249">
        <property type="protein sequence ID" value="CCZ84589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00450"/>
    <dbReference type="EnsemblBacteria" id="CCZ84589">
        <property type="protein sequence ID" value="CCZ84589"/>
        <property type="gene ID" value="BN566_00450"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034335">
        <property type="term" value="F:DNA negative supercoiling activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00187">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.268.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.199.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01897">
        <property type="entry name" value="GyrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005743">
        <property type="entry name" value="GyrA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006691">
        <property type="entry name" value="GyrA/parC_rep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035516">
        <property type="entry name" value="Gyrase/topoIV_suA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013760">
        <property type="entry name" value="Topo_IIA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013758">
        <property type="entry name" value="Topo_IIA_A/C_ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013757">
        <property type="entry name" value="Topo_IIA_A_a_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002205">
        <property type="entry name" value="Topo_IIA_dom_A"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03989">
        <property type="entry name" value="DNA_gyraseA_C"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00521">
        <property type="entry name" value="DNA_topoisoIV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00434">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101904">
        <property type="entry name" value="SSF101904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56719">
        <property type="entry name" value="SSF56719"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="TOP4c" evidence="10">
        <location>
            <begin position="19"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="836"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GyrA-box" evidence="8">
        <location>
            <begin position="557"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="846"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="8">
        <location>
            <position position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ84589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="874" mass="98416" checksum="E0DD3D0D66D9011E" modified="2013-07-24" version="1">MNEEKTTALENRDGKITPVDIGQEMQKSFLEYAMSVIVARALPDVRDGLKPVHRRILYTMYENNLTPDRPYHKCADTVGSVLGRYHPHGDASVYDALVRLAQNFSLRYVLVDGQGNFGSVDGDPPAAYRYTEARMSKIALEMLTDIQKETVPFVPNYDERLKEPAVLPSRYPNLLVNGSTGIAVGMATNIPPHNLGEVIDGILLLMEQPDCTLEELMQCIKGPDFPTGGIIMGYAGMRAAYATGRGKITLRGKTSFETVRGRESIIITEIPYMVNKARLVESIADHAKDNRIEGIYHIQDESSREGMRVVIELKKDANPQIVLNKLFSYTQLQDTIGVNLLALVNGEPKVLTLKQILEQYLQFQVEVITNRTKYELKKAEKRAHLLQGFVVAIDHIDEVISILRSSRTVVEGKQRLMERFKDMDLTALLDRAQYDTEKYQMEQQIGLSDEQADAIVQMRLGQLTGMERQKVTDELYQILAKISDYLDILSDEQRVYGIIREDLESIRNRFGDPRRTQIEMVDGEVDIEDLIPVEDCVVTFTESGYMKRMPVDVYKTQRRGGRGVSGMKQREADFVNEMFISSTHDHILFISNYGMMYRLKCYEIPEGSKASRGFNIVNLLPLKEGEKIAAMLRTKDFDEGKYLVTVTRQGKIKRTALPAYKNVRKNGLIAVGLEEGDEIAGVRLTDGSARLFVATKHGMIIRMEETCIRPQGRSAHGVKAITLREGDVVVSIARERAGASLLTVTENGYGRRSELEQYRIQNRGGYGLQNYKVDAERGMVCGIKVVDETDDILLISTDGIVIRVYCADIRLMGRTAKGVRIMRVTNENQVVAFSRTEHDETEACSQIEETAEDAVETAPEDPVDLPETTEETNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5V993</accession>
    <name>R5V993_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00537</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000267">
        <property type="protein sequence ID" value="CCZ84684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84684">
        <property type="protein sequence ID" value="CCZ84684"/>
        <property type="gene ID" value="BN566_00537"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="221" mass="25428" checksum="B71A3E5676132E6D" modified="2013-07-24" version="1">MATWVIHLRVADALRNRWQPLQTLPELPFVVGNLAPDGGVEVSPHVYVPDSATTHWTPTGKKRDIDAEAFCRAYLQNPALTPEKTAFYRGYEAHLRTDVEWIRQILKPLKQKLGIAHFTGTPYDGLLRQNLTYLEQAFCRQAKSFPAWELAQQTKAFPNDVLPYYAEDTLTLKLQELRERYAEPVAPPEPYLVTAEQMDAFVRQTVEQIVSVWQKKENQFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>R5VH98</accession>
    <name>R5VH98_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="14 15">Dihydroxy-acid dehydratase</fullName>
            <shortName evidence="15">DAD</shortName>
            <ecNumber evidence="14 15">4.2.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ilvD</name>
        <name evidence="16" type="ORF">BN566_00721</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Functions in the biosynthesis of branched-chain amino acids. Catalyzes the dehydration of (2R,3R)-2,3-dihydroxy-3-methylpentanoate (2,3-dihydroxy-3-methylvalerate) into 2-oxo-3-methylpentanoate (2-oxo-3-methylvalerate) and of (2R)-2,3-dihydroxy-3-methylbutanoate (2,3-dihydroxyisovalerate) into 2-oxo-3-methylbutanoate (2-oxoisovalerate), the penultimate precursor to L-isoleucine and L-valine, respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(2R)-2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:24809"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:49072"/>
            <dbReference type="EC" id="4.2.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:24810"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(2R,3R)-2,3-dihydroxy-3-methylpentanoate = (S)-3-methyl-2-oxopentanoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:27694"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:49258"/>
            <dbReference type="EC" id="4.2.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="15">Binds 1 [2Fe-2S] cluster per subunit. This cluster acts as a Lewis acid cofactor.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="13 15">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="12 15">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the IlvD/Edd family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.9" evidence="14 15"/>
    <dbReference type="EMBL" id="CBAR010000029">
        <property type="protein sequence ID" value="CCZ82921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00721"/>
    <dbReference type="EnsemblBacteria" id="CCZ82921">
        <property type="protein sequence ID" value="CCZ82921"/>
        <property type="gene ID" value="BN566_00721"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00057"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00061"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004160">
        <property type="term" value="F:dihydroxy-acid dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00012">
        <property type="entry name" value="IlvD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042096">
        <property type="entry name" value="Dihydro-acid_dehy_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004404">
        <property type="entry name" value="DihydroxyA_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000581">
        <property type="entry name" value="DiOHA_6PGluconate_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020558">
        <property type="entry name" value="DiOHA_6PGluconate_deHydtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037237">
        <property type="entry name" value="IlvD/EDD_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00920">
        <property type="entry name" value="ILVD_EDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143975">
        <property type="entry name" value="SSF143975"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00110">
        <property type="entry name" value="ilvD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00886">
        <property type="entry name" value="ILVD_EDD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00887">
        <property type="entry name" value="ILVD_EDD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 15" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3 15" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10 15" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="7 15" id="KW-0408">Iron</keyword>
    <keyword evidence="8 15" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 15" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="468"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" description="via carbamate group" evidence="15">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="442"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="15">
        <location>
            <position position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CCZ82921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="553" mass="58532" checksum="CAC8146048540420" modified="2013-07-24" version="1">MAGNFYCDGVGKAPQRSLFHALGMTKEEMERPLVGIVSSYNEIVPGHMNIDKIVEAVKMGVAMAGGTPVVFPAIAVCDGIAMGHVGMKYSLVTRDLIADSTECMALAHHFDALVMIPNCDKNVPGLLMAAARINVPTVFVSGGPMLAGHVKGKKTSLSSMFEAVGSYDAGKFTLEDVEEFENNACPTCGSCSGMYTANSMNCLTEVLGMGLKGNGTIPAVYSERLILAKKAGMAVMDLYRKNIRPRDIMTAEAFENALTADMALGCSTNSMLHIPAIANECGISINLDMANAISAKTPNLCHLAPAGHAYMEDLNAAGGVYAVLNELAKKNLIHTDTMTVTGKTLGENIQGCINKNPDIIRPIDNPYSPTGGIAVLKGNLAPDRCVVKRSAVAAEMMQHRGPAKVFNSEEEAIAVIRSGGIQKGDVVVIRYEGPAGGPGMREMLSPTSAIAGMGLDKDVALITDGRFSGATRGAAIGHVSPEAVSGGNIAYVQDGDMISIDIPNYTITLEVSDEELEKRKQTMPILKKDHLTGYLKRYAKLVSSADKGAIINR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5VHG1</accession>
    <name>R5VHG1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00771</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000033">
        <property type="protein sequence ID" value="CCZ82971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82971">
        <property type="protein sequence ID" value="CCZ82971"/>
        <property type="gene ID" value="BN566_00771"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="84" mass="9549" checksum="8F7195CA4FD649A5" modified="2013-07-24" version="1">MEIFKVGKDTGNSASTQKEETQKFFFKVFGFLILLIITVVGTALFLPKKPFISMIISAFTLGLLIVFMVLVELGIFWRKKGEER</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>R5VHI0</accession>
    <name>R5VHI0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN566_00108</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the OXA1/ALB3/YidC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000035">
        <property type="protein sequence ID" value="CCZ82986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00108"/>
    <dbReference type="EnsemblBacteria" id="CCZ82986">
        <property type="protein sequence ID" value="CCZ82986"/>
        <property type="gene ID" value="BN566_00108"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032977">
        <property type="term" value="F:membrane insertase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028055">
        <property type="entry name" value="Membr_insert_YidC/Oxa1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001708">
        <property type="entry name" value="YidC/ALB3/OXA1/COX18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428">
        <property type="entry name" value="PTHR12428"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02096">
        <property type="entry name" value="60KD_IMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03592">
        <property type="entry name" value="yidC_oxa1_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="191"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="232"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="254"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="312"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="312"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="334"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ82986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="360" mass="41505" checksum="048E71040E450C3E" modified="2013-07-24" version="1">MRLIYQLVNNYGVAIVLFTIITKILLFPISYKQQKNTLRMQLIQPKLKKLQAKYKDKPQELQQAQMHLYQEEHVNPMSSCLPMFLQFFLLFGVLDVVYKPLTHILHYSKSVISRVVEISTQVLSANDIKQYTGLRQELGVLKAFQENPSAFGNVDTFDVSKVSDFMEHFTVFGVNLGDTPKWHPEVWNTSAVILFCIPLLSGILQLIMTIYMQIMQKKRGTAPEQNMGCMNVMLYVMPLFSVWFAFQVSAGVGFYWACSSFFSLLQTIGLNLYFTPERGAKIIEKENKKMEARYAAGKQSFTERVMNQNAGAARVQETEKTKNMSNRELSSYNKQKIQEARKRMAEKYGETYEEDSSDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5VHZ6</accession>
    <name>R5VHZ6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00954</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000069">
        <property type="protein sequence ID" value="CCZ83171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83171">
        <property type="protein sequence ID" value="CCZ83171"/>
        <property type="gene ID" value="BN566_00954"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004407116" evidence="3">
        <location>
            <begin position="26"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="331"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="301"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="312"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="357" mass="38996" checksum="219AA3DB1D65B63B" modified="2013-07-24" version="1">MNTKKMLAILAALSMLGTTGLTVGAEDTAATEPAVETVVTTEAEAETAVSEIAETRTETTAADEAKMEVNETTVTEETAITTTIVQSEEPTSTTTAVKSDADGVETTVAVTGTESASVTTTTTYSAEETSKIMEELQKQIDNLIDEIQSGSNPDLLPDEENVEMLPDYSSDNILQLEDGTWIGTYHTYYVDEEGYTYQVVYYVKTDGTKDIFYVTYYDDFDHNDALVCSDNPFYDEFYVQFTTFYKTLENTSDYYVLYCATDAGVNPTMYVVVEMTDGNLVFYAISQDGIQQVDKIPTFEFDDDSKKPNDSARPTAAVQKSTSSTPKTGDMATAPAVAIGLTLTAAGVVAFLNRKYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>R5VI11</accession>
    <name>R5VI11_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GntR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00974</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000070">
        <property type="protein sequence ID" value="CCZ83191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00974"/>
    <dbReference type="EnsemblBacteria" id="CCZ83191">
        <property type="protein sequence ID" value="CCZ83191"/>
        <property type="gene ID" value="BN566_00974"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="1">
        <location>
            <begin position="7"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="131" mass="15182" checksum="6DA09B1825C9AD88" modified="2013-07-24" version="1">MEFQNNYPIYLQIADDIKRRIVIGELHPGDKLPSNTDLAVAYTVNPNTVQRIYRQLEVEGITFTRRGVGTFLSEDPELAERLRSTILEQEVTAFLKKMQQYHFSKAALFTAMEQQLEQAEKKEETETTDKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5VI93</accession>
    <name>R5VI93_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted redox protein regulator of disulfide bond formation</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01062</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfur carrier protein TusA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01062"/>
    <dbReference type="EnsemblBacteria" id="CCZ83281">
        <property type="protein sequence ID" value="CCZ83281"/>
        <property type="gene ID" value="BN566_01062"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001455">
        <property type="entry name" value="TusA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036868">
        <property type="entry name" value="TusA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01206">
        <property type="entry name" value="TusA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64307">
        <property type="entry name" value="SSF64307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UPF0033" evidence="2">
        <location>
            <begin position="9"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="81" mass="8942" checksum="F6F33F951097793B" modified="2013-07-24" version="1">MADFVIDDTVDITDVVCPVTFVKAKVALEELDDGQVLSIKMNDGEPVQNVPRSIKEEGHKILKLIDNEDGTYNLIVKKVGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5VIF4</accession>
    <name>R5VIF4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">dTTP/UTP pyrophosphatase</fullName>
            <shortName evidence="2">dTTPase/UTPase</shortName>
            <ecNumber evidence="2">3.6.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Nucleoside triphosphate pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Nucleotide pyrophosphatase</fullName>
            <shortName evidence="2">Nucleotide PPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00123</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Nucleoside triphosphate pyrophosphatase that hydrolyzes dTTP and UTP. May have a dual role in cell division arrest and in preventing the incorporation of modified nucleotides into cellular nucleic acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>H2O + UTP = diphosphate + H(+) + UMP</text>
            <dbReference type="Rhea" id="RHEA:29395"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="EC" id="3.6.1.9"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>dTTP + H2O = diphosphate + dTMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28534"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37568"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="EC" id="3.6.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Maf family. YhdE subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.9" evidence="2"/>
    <dbReference type="EMBL" id="CBAR010000090">
        <property type="protein sequence ID" value="CCZ83341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00123"/>
    <dbReference type="EnsemblBacteria" id="CCZ83341">
        <property type="protein sequence ID" value="CCZ83341"/>
        <property type="gene ID" value="BN566_00123"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036218">
        <property type="term" value="F:dTTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035529">
        <property type="term" value="F:NADH pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036221">
        <property type="term" value="F:UTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00555">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00528">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003697">
        <property type="entry name" value="Maf-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43213">
        <property type="entry name" value="PTHR43213"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02545">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006305">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="SSF52972"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00172">
        <property type="entry name" value="maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="68"/>
        </location>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="2">
        <location>
            <position position="11"/>
        </location>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="2">
        <location>
            <position position="69"/>
        </location>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="2">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="196" mass="21509" checksum="38565944267A517B" modified="2013-07-24" version="1">MPYILASASPRRQELLHKITDDFIVQPADVDETIPDDILPEDAAVYLSHIKAEAVSNQYPGSIVIGCDTIVLLNDQILGKPHSPEQAREMLQMLSGNRHQVITGTTVTDGSHTISFASETAVQFYPLSEAEISAYLETGEPFDKAGAYGIQGKGSLLIQGIQGDYFTVMGLPVAKLYRVLQQFETNFKKEASNLHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJ47</accession>
    <name>R5VJ47_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative AIP processing-secretion protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01275</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000119">
        <property type="protein sequence ID" value="CCZ83506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83506">
        <property type="protein sequence ID" value="CCZ83506"/>
        <property type="gene ID" value="BN566_01275"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009372">
        <property type="term" value="P:quorum sensing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006741">
        <property type="entry name" value="AgrB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04647">
        <property type="entry name" value="AgrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00793">
        <property type="entry name" value="AgrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0673">Quorum sensing</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="86"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="131"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="155"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="194"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="217"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="245" mass="27270" checksum="59A4A4B87C8CDA73" modified="2013-07-24" version="1">MNETDFQKGITGSSFHCKKIIFFLGKFPLYLVAISAEDTEIREEDAEILMLQTCSEKFAKKLIQAEIIPESDADIYVFGCYQFGMMLLNIVTTIGLGILFRLFLPCVLLNLSFIPIRISAGGHHADSPVKCYVLSTLLIAALLAILKWVSVSLLAAWILILAASAVLLWLAPVETENNPLDKTEVRVYRRRTRIVLGIELLAALLATLLHRNFLVTIIGFGLLTASFMLVVGACANLKQHWKRCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJG8</accession>
    <name>R5VJG8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative phage head-tail adaptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01412</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000148">
        <property type="protein sequence ID" value="CCZ83676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01412"/>
    <dbReference type="EnsemblBacteria" id="CCZ83676">
        <property type="protein sequence ID" value="CCZ83676"/>
        <property type="gene ID" value="BN566_01412"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="104" mass="12193" checksum="B35FC7C9B13AB875" modified="2013-07-24" version="1">MDDVIELVKQHLYRDDCGVERLAEESKRTVFCSVQSASRAEFFAAMQAGLKPSFIVQINPIEYDCEGIAVYHEKRYLIYRTYQKNMDVLELYLKEEVGIQNDLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>R5VKD2</accession>
    <name>R5VKD2_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Riboflavin biosynthesis protein RibD</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="10">Diaminohydroxyphosphoribosylaminopyrimidine deaminase</fullName>
                <shortName evidence="10">DRAP deaminase</shortName>
                <ecNumber evidence="10">3.5.4.26</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="10">Riboflavin-specific deaminase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="10">5-amino-6-(5-phosphoribosylamino)uracil reductase</fullName>
                <ecNumber evidence="10">1.1.1.193</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="10">HTP reductase</fullName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BN566_01705</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Converts 2,5-diamino-6-(ribosylamino)-4(3h)-pyrimidinone 5'-phosphate into 5-amino-6-(ribosylamino)-2,4(1h,3h)-pyrimidinedione 5'-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + H(+) + H2O = 5-amino-6-(5-phospho-D-ribosylamino)uracil + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:21868"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:58453"/>
            <dbReference type="ChEBI" id="CHEBI:58614"/>
            <dbReference type="EC" id="3.5.4.26"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>5-amino-6-(5-phospho-D-ribitylamino)uracil + NADP(+) = 5-amino-6-(5-phospho-D-ribosylamino)uracil + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:17845"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58421"/>
            <dbReference type="ChEBI" id="CHEBI:58453"/>
            <dbReference type="EC" id="1.1.1.193"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10 13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="10 13">Binds 1 zinc ion.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-6-(D-ribitylamino)uracil from GTP: step 2/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 10">Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-6-(D-ribitylamino)uracil from GTP: step 3/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 10">In the C-terminal section; belongs to the HTP reductase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">In the N-terminal section; belongs to the cytidine and deoxycytidylate deaminase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.26" evidence="10"/>
    <dbReference type="EC" id="1.1.1.193" evidence="10"/>
    <dbReference type="EMBL" id="CBAR010000178">
        <property type="protein sequence ID" value="CCZ83976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01705"/>
    <dbReference type="EnsemblBacteria" id="CCZ83976">
        <property type="protein sequence ID" value="CCZ83976"/>
        <property type="gene ID" value="BN566_01705"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00401"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008703">
        <property type="term" value="F:5-amino-6-(5-phosphoribosylamino)uracil reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008835">
        <property type="term" value="F:diaminohydroxyphosphoribosylaminopyrimidine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016192">
        <property type="entry name" value="APOBEC/CMP_deaminase_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024072">
        <property type="entry name" value="DHFR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004794">
        <property type="entry name" value="Eubact_RibD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011549">
        <property type="entry name" value="RibD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002734">
        <property type="entry name" value="RibDG_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01872">
        <property type="entry name" value="RibD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006769">
        <property type="entry name" value="RibD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53597">
        <property type="entry name" value="SSF53597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="SSF53927"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00326">
        <property type="entry name" value="eubact_ribD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00227">
        <property type="entry name" value="ribD_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00903">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 10 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="10 12" id="KW-0521">NADP</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0686">Riboflavin biosynthesis</keyword>
    <keyword evidence="8 10 13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="14">
        <location>
            <begin position="2"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="296"/>
            <end position="302"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR006769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR006769-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR006769-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR006769-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CCZ83976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="362" mass="38994" checksum="50C78CC8650ACD33" modified="2013-07-24" version="1">MTEHTFYMQRAIALAKRGCGKTNPNPMVGAVIVKGHRIIGEGWHVQYGALHAERHALANCTESPESATLYVTLEPCCHYGKQPPCTEAVIQSGIKTVYVGSRDPNPLVAGKGIQQLRDAGITVYTDFLRKECDAINPIFFHYITTKLPYCIVKYAMTMDGAIACGNGASKWVTGELARKDVQQTRKAVAAILVGIQTVLADDPLLTCRIDPACNPVRIVCDSHLRIPLTSKLVQTAKDVPLIVMTCSDDQEQIQALQQKNVTVCVLPADETGRPAFSAILQKIGALKLDSVLIEGGASIHASALKTGLVQQVQVYLAPKCFGGDGLSPIAPLGVTDPMQAISFSAPIVTPLGQDIRLDYFAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5VKM1</accession>
    <name>R5VKM1_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0237 protein BN566_01772</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01772</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0237 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000182">
        <property type="protein sequence ID" value="CCZ84046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01772"/>
    <dbReference type="EnsemblBacteria" id="CCZ84046">
        <property type="protein sequence ID" value="CCZ84046"/>
        <property type="gene ID" value="BN566_01772"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04872">
        <property type="entry name" value="ACT_1ZPV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01054">
        <property type="entry name" value="UPF0237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022986">
        <property type="entry name" value="UPF0237_ACT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACT" evidence="2">
        <location>
            <begin position="4"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="89" mass="9840" checksum="52E9D64FE3BE2029" modified="2013-07-24" version="1">MRAVITVIGKDNVGILHKVSGVCAEYQANVLEVTQSVLQDMFAMIMMVDITKMTADFAKLSDQLTALGEELGLSVHCMHEDIFNAMHRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5VKX4</accession>
    <name>R5VKX4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ABC-type uncharacterized transport system</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01906</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01906"/>
    <dbReference type="EnsemblBacteria" id="CCZ84181">
        <property type="protein sequence ID" value="CCZ84181"/>
        <property type="gene ID" value="BN566_01906"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019196">
        <property type="entry name" value="ABC_transp_unknown"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09822">
        <property type="entry name" value="ABC_transp_aux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="464"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="ABC_transp_aux" evidence="2">
        <location>
            <begin position="43"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="489" mass="53716" checksum="70188EFE25504397" modified="2013-07-24" version="1">MEEKKFHNFKNKKFSSIAWIMAVLVIVIAVILNMIVSQLDLGWDVSPNKQYSLGKTTTAYLDELDQNGTKIDFYVLAKMDDIKDSVDTLALYRALKEYDEHDCINLIDIDPNSDEATMEKINPDNALTLNTGDMVLVCGDTKKRIPGNTMYTTNSDDNGNAVSQTFNGENILTGAIKSVVDGFTPTVYFLTGHGEKSADDNYTQFKKNLQNYNYAAKSLLLSDVDAVPSDAAMVLVAAPTSDISDSDKEKLDAYLNDGGNLSLLMSPNAGKFNYTNLDLIMEEFSIIMDYDTVKETDASMHVSGDDSTIQCNLVELSSDSEAADLTSSLINQGLVPYMTNSRSFYFDTDTTGTLTTAELLTTNDTAVGEPNGGAYDTKKITNSELMLAGYSQSNTKNNAKLAVFGNADFLDDTHLQDSRYIVAVYLYLSTISWMYDTDVDMGIDSKSTVYDEISLPDANSARSLMIVFAALPVAIIIIGVGVWVKRRNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5VL79</accession>
    <name>R5VL79_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_00214</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000209">
        <property type="protein sequence ID" value="CCZ84301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84301">
        <property type="protein sequence ID" value="CCZ84301"/>
        <property type="gene ID" value="BN566_00214"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005247">
        <property type="term" value="F:voltage-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014743">
        <property type="entry name" value="Cl-channel_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="Cl-channel_volt-gated"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00654">
        <property type="entry name" value="Voltage_CLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00762">
        <property type="entry name" value="CLCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81340">
        <property type="entry name" value="SSF81340"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="157"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="188"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="229"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="266"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="306"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="331"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="356"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="390"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ84301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="429" mass="46391" checksum="009FCB66E85B9D07" modified="2013-07-24" version="1">MKGNRYWVRVRARVLHYGIHVLSHLRLLIRWIFLALVAGVVAGGVGAAFAWGMTAVTNLRTQHPWLLLLLPVSGLAIVALYRACHFEKDSGTNTVLAMIHAEKEIPARMAPLIFISTMLTHLCGGSSGREGAALQIGGSLGNLLGRVMRLQPDDRRVLVMCGMSAAFSAVFGTPLAAVFFALEVGSVGIIYYAALVPCVTASFCADAIAKFFRVESESYPLIVSMEWSVAPLAATALLGILCAGLSVLFCISLHETGCLLKKKIPNAYLRIAVVGVVLVILGLLPGNSDYLGAGMPILIRALSGKVVWYAFLMKLLFTVLTMAAGFKGGEIVPSFFIGATFGCLYGSILHLSPSMFAALGMIAVFCGVTNCPLTSLLIAFELFGMEAMPYFLICIAVSYMLSGYYGLYHSQRIVYSKYKPKYVNRKTRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>R5VLA6</accession>
    <name>R5VLA6_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5 7">30S ribosomal protein S9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpsI</name>
        <name evidence="8" type="ORF">BN566_00239</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the universal ribosomal protein uS9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000213">
        <property type="protein sequence ID" value="CCZ84326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00239"/>
    <dbReference type="EnsemblBacteria" id="CCZ84326">
        <property type="protein sequence ID" value="CCZ84326"/>
        <property type="gene ID" value="BN566_00239"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00532_B">
        <property type="entry name" value="Ribosomal_S9_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000754">
        <property type="entry name" value="Ribosomal_S9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023035">
        <property type="entry name" value="Ribosomal_S9_bac/plastid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020574">
        <property type="entry name" value="Ribosomal_S9_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569">
        <property type="entry name" value="PTHR21569"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00380">
        <property type="entry name" value="Ribosomal_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00360">
        <property type="entry name" value="RIBOSOMAL_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ84326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="133" mass="14896" checksum="EC220196FD2EB9B5" modified="2013-07-24" version="1">MYESKVKYYYGTGRRKHSVARVRLYPGNGTITINGRGIDDYFGLDTLKLIVRQPLALTENLENMDVVCTVAGGGVTGQAGAIRHGVARALLEFNPELRPVLKKAGFLTRDPRMKERKKYGLKAARRAPQFSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5VLX8</accession>
    <name>R5VLX8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8 11">50S ribosomal protein L22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rplV</name>
        <name evidence="12" type="ORF">BN566_00062</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">The globular domain of the protein is located near the polypeptide exit tunnel on the outside of the subunit, while an extended beta-hairpin is found that lines the wall of the exit tunnel in the center of the 70S ribosome.</text>
    </comment>
    <comment type="function">
        <text evidence="7">This protein binds specifically to 23S rRNA; its binding is stimulated by other ribosomal proteins, e.g. L4, L17, and L20. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome.</text>
    </comment>
    <comment type="function">
        <text evidence="8 11">This protein binds specifically to 23S rRNA; its binding is stimulated by other ribosomal proteins, e.g., L4, L17, and L20. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8 10">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 9">Belongs to the universal ribosomal protein uL22 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000241">
        <property type="protein sequence ID" value="CCZ84516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00062"/>
    <dbReference type="EnsemblBacteria" id="CCZ84516">
        <property type="protein sequence ID" value="CCZ84516"/>
        <property type="gene ID" value="BN566_00062"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00336">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01331_B">
        <property type="entry name" value="Ribosomal_L22_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001063">
        <property type="entry name" value="Ribosomal_L22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018260">
        <property type="entry name" value="Ribosomal_L22/L17_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036394">
        <property type="entry name" value="Ribosomal_L22/L17_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005727">
        <property type="entry name" value="Ribosomal_L22_bac/chlpt-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13501">
        <property type="entry name" value="PTHR13501"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00237">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54843">
        <property type="entry name" value="SSF54843"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01044">
        <property type="entry name" value="rplV_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00464">
        <property type="entry name" value="RIBOSOMAL_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 8 9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 8 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 8 10" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CCZ84516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="111" mass="12435" checksum="447F88DAC2989E5D" modified="2013-07-24" version="1">MEAKAYLRNARISPRKVQIVLDLIRNKPVDVALATLDLTPKAASPLLEKLLKSAIANAENNFSMNGDALYVAECYVTPGPIMKRIQPRAQGRAFRIYKRTSHITVVLKEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5VM24</accession>
    <name>R5VM24_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH arsR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00442</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000247">
        <property type="protein sequence ID" value="CCZ84581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00442"/>
    <dbReference type="EnsemblBacteria" id="CCZ84581">
        <property type="protein sequence ID" value="CCZ84581"/>
        <property type="gene ID" value="BN566_00442"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00778">
        <property type="entry name" value="HTHARSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="7"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="99" mass="11262" checksum="3A39111B1EA81DE1" modified="2013-07-24" version="1">MTDILAPDDPALQSMADLFKVFGDPTRVRILFALFQQEACVQEIANRLGMTQSAISHQLRILKQARLVSSRREGKSIFYALADDHIYTIFRQALEHIAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5VM75</accession>
    <name>R5VM75_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00499</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000264">
        <property type="protein sequence ID" value="CCZ84646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84646">
        <property type="protein sequence ID" value="CCZ84646"/>
        <property type="gene ID" value="BN566_00499"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004395264" evidence="2">
        <location>
            <begin position="29"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="261" mass="28987" checksum="5D639B37B2C799F0" modified="2013-07-24" version="1">MKKALSICLFLSLMAGCLLTGCDRTSTANSSSVAETTTATTATEAETTAELEAEETEAETETTAEESADAEDAETTTAAKEKDTSKDGNLWADFDNMSFKINGKTYTLGKTTLQEMIDDGVPFDENDLANAGNNINPRYESSSFEIKLGERSFAQVRVLNDSSDNKKASECFIDNVYMPIYDEPQDILEFNFPLTMTQEELLANSGEPDPDNIYTYQSDDSDYHSESYTYEKEAEKYYGNSSYKFEFANDVLAYVTISYTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5VM88</accession>
    <name>R5VM88_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00514</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000267">
        <property type="protein sequence ID" value="CCZ84661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84661">
        <property type="protein sequence ID" value="CCZ84661"/>
        <property type="gene ID" value="BN566_00514"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="58" mass="6604" checksum="6B606F220C782435" modified="2013-07-24" version="1">MSKEKDSGKKERLSMFYKVAPVVGALVLAMLSAVFIVCRTLSNRSHHEKWKDYDECGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>R5VQB4</accession>
    <name>R5VQB4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Coproporphyrinogen dehydrogenase HemZ</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00577</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000007">
        <property type="protein sequence ID" value="CCZ82772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00577"/>
    <dbReference type="EnsemblBacteria" id="CCZ82772">
        <property type="protein sequence ID" value="CCZ82772"/>
        <property type="gene ID" value="BN566_00577"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034505">
        <property type="entry name" value="Coproporphyrinogen-III_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023995">
        <property type="entry name" value="HemZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13932">
        <property type="entry name" value="PTHR13932"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13932:SF1">
        <property type="entry name" value="PTHR13932:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00310">
        <property type="entry name" value="oxygen-independent_coproporphy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03994">
        <property type="entry name" value="rSAM_HemZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="176"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ82772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="509" mass="58453" checksum="270CA7C151A07BDB" modified="2013-07-24" version="1">MKETTQTNLPTFPVELYGHSLKYEIECMLKIFLPATKFVFSYETASLLRSAEQGAFLQLKQGKQFTWFFCVLRYQGKYRRCAAKLPKCTPEENKQTCEYQFSRMLFTLLQDITGIEVPWGLLTGIRPVRKAFSMMEESGESLDAVMEQLRTVYLVSPEKLSLLRKTAEVQLPLLRQTPKRSVGFYISIPFCPTRCSYCSFVSHSIAQAKHLMPEYIRLLCRELEIWGDIIQKLQVTVDTIYFGGGTPTSISAEALEQLLQTVVRVFDLSKVREFCVEAGRPDTITREKLEVLKRYGVDRISVNPQTMEDAVLQQIGRKHTSAQIAEAFQLAREVGFTNINMDLIAGLPHDTPEGFHRTLEKVLALNPDSITVHTLTLKRAATLFSDRQAQIGNPVREMVRDSIALLPEHGYQPYYMYRQKNTIDNQENVGYARAGKESYYNMLIMDESQSIFGAGCAASTKIVEPDCGRITRIHNYKFPYEYIRQFDQLMEKKQQVLEIYQRMEAAEQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5VQE9</accession>
    <name>R5VQE9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Spore coat-associated protein JA CotJA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00622</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000011">
        <property type="protein sequence ID" value="CCZ82817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00622"/>
    <dbReference type="EnsemblBacteria" id="CCZ82817">
        <property type="protein sequence ID" value="CCZ82817"/>
        <property type="gene ID" value="BN566_00622"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020256">
        <property type="entry name" value="Spore_coat_CotJA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11007">
        <property type="entry name" value="CotJA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ82817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="87" mass="10170" checksum="E0C392734E88BF85" modified="2013-07-24" version="1">MQWNHQGMELEFQPEMAWDHFHLFEDTPQPPEQTGMRTPLPEETPIGMAYVPYQQWDTTFSAEEGFPKGTIFPQLYKPFCPGGERHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5VR72</accession>
    <name>R5VR72_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Bac_transf domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00830</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial sugar transferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000040">
        <property type="protein sequence ID" value="CCZ83037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00830"/>
    <dbReference type="EnsemblBacteria" id="CCZ83037">
        <property type="protein sequence ID" value="CCZ83037"/>
        <property type="gene ID" value="BN566_00830"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003362">
        <property type="entry name" value="Bact_transf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02397">
        <property type="entry name" value="Bac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="Bac_transf" evidence="3">
        <location>
            <begin position="10"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="209" mass="24066" checksum="F75119F953F884DF" modified="2013-07-24" version="1">MHTFYRRYGKRLLDIILGILGCIPACPVMLIVIIAMFFFDRGTIFFVQERAGKRDVNGNPTTFRMHKFRTMKMDTPHDVPTDQFEHADQYITPLGKFLRKSSLDELPQIFDILRGDMSFVGPRPALPNQTRLLELRHENGSDQLKPGLFGLAQKNGRDQLLETEKAAYDGEYAEKLSFWMDCKSFFGCIFNSVLQKNIVEGKQPHSTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5VRG6</accession>
    <name>R5VRG6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">HAD-superfamily hydrolase subfamily IA variant 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00939</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000064">
        <property type="protein sequence ID" value="CCZ83152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00939"/>
    <dbReference type="EnsemblBacteria" id="CCZ83152">
        <property type="protein sequence ID" value="CCZ83152"/>
        <property type="gene ID" value="BN566_00939"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="222" mass="24781" checksum="D0FF481703D3FD04" modified="2013-07-24" version="1">MSYRAVLWDLDGTLTDSQEGILRCTQYALKACGIEESDPQKLIRFIGPPLAYSFATFYQMQEPMVSFAVEKYRERYGTVGWKENRLYPGIAECLKALKAAGYQVAMATAKPEFYAKKIVAFFELMPYFDAVIGSSMEHSDASKAYLMQRAMQQLHIPEAQKEQVVMIGDRKFDAEGAAACGLPFIGVHYGYAPAGELESYPSVYLADTVSDLKTFLLEHAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5VRM5</accession>
    <name>R5VRM5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00990</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000071">
        <property type="protein sequence ID" value="CCZ83207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00990"/>
    <dbReference type="EnsemblBacteria" id="CCZ83207">
        <property type="protein sequence ID" value="CCZ83207"/>
        <property type="gene ID" value="BN566_00990"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008841">
        <property type="entry name" value="Siphovirus-type_tail"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05709">
        <property type="entry name" value="Sipho_tail"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="113" mass="12341" checksum="8A6C980312C6D8E0" modified="2013-07-24" version="1">MTINNSGDEVGFTLEISGGPAKNPTIYNALTDEYMQISGDIQKGDVITITTKTGNKTVLLEREGVVTNIINRLVSGSTWLNLKAGENKFYVRASNGLNNIKVRLIHRNAYLGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>R5VRU6</accession>
    <name>R5VRU6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Dissimilatory sulfite reductase (Desulfoviridin) alpha and beta subunits</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_01063</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01063"/>
    <dbReference type="EnsemblBacteria" id="CCZ83282">
        <property type="protein sequence ID" value="CCZ83282"/>
        <property type="gene ID" value="BN566_01063"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.413.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005117">
        <property type="entry name" value="NiRdtase/SiRdtase_haem-b_fer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036136">
        <property type="entry name" value="Nit/Sulf_reduc_fer-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006067">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045169">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045854">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006066">
        <property type="entry name" value="NO2/SO3_Rdtase_FeS/sirohaem_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11493">
        <property type="entry name" value="PTHR11493"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01077">
        <property type="entry name" value="NIR_SIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03460">
        <property type="entry name" value="NIR_SIR_ferr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00397">
        <property type="entry name" value="SIROHAEM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55124">
        <property type="entry name" value="SSF55124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56014">
        <property type="entry name" value="SSF56014"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00365">
        <property type="entry name" value="NIR_SIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="7">
        <location>
            <begin position="156"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="7">
        <location>
            <begin position="185"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="287" mass="31626" checksum="8E469E3888F4AE93" modified="2013-07-24" version="1">MAVDYATLKKGGFMRQKQKNHFSLRLRVVGGNLTATQLAKIAEVAEKFGDGHVHLTSRQSVEIPFVKLEQIEEVKKALSEGGVEPGVCGPRVRTITACQGEAICPSGCIDTYALAKELDDRYFARELPHKFKFGITGCQNNCLKSEENDLGIKGGIQVSWREEDCIQCGVCVKACRSNAITLEDGKISVDESKCNFCGRCYKACPTEAWDTVHGYIVSFGGLFGNSISKGEAIIPFLPDKETLLKVCDATIQFFADHAKPGERFKFTLDRVGRESFENLIQEVVKNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5VRW4</accession>
    <name>R5VRW4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cell shape-determining protein MreB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">mreB</name>
        <name evidence="3" type="ORF">BN566_01083</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Forms membrane-associated dynamic filaments that are essential for cell shape determination. Acts by regulating cell wall synthesis and cell elongation, and thus cell shape. A feedback loop between cell geometry and MreB localization may maintain elongated cell shape by targeting cell wall growth to regions of negative cell wall curvature.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Forms polymers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="2">Membrane-associated.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the FtsA/MreB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01083"/>
    <dbReference type="EnsemblBacteria" id="CCZ83302">
        <property type="protein sequence ID" value="CCZ83302"/>
        <property type="gene ID" value="BN566_01083"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd10225">
        <property type="entry name" value="MreB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02207">
        <property type="entry name" value="MreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004753">
        <property type="entry name" value="MreB"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01652">
        <property type="entry name" value="SHAPEPROTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00904">
        <property type="entry name" value="mreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0133">Cell shape</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="12"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="155"/>
            <end position="157"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="340" mass="36914" checksum="D8FB471ECD4C7CD7" modified="2013-07-24" version="1">MATTDIGIDLGTANTVITLGRKGVVLREPSAIAYHLRTKEVLAVGMAAYEMIGKTPDYITVVRPLSNGVISDDRMAQYMIREFILRVTGKRLLKPRIIICVPSFITDVEKRAVVEAAMSAGSRKAYLIDEPIAALIGAGVNIGKARGNMIVDIGGGTTDVAIVSMNGIVTSHSIKVAGNTLDQAIIRYVQTKYKLLIGEHMAERAKIQLTNLFDPREDVTMYIKGRNLVSGMPEQIEISETEIFEAVEDEISDIIEAIKTVLEETPPELVGDIYENGVLMAGGGAMLGGLRKLVEHTLKVRCVVAKDAMNCVAKGTAIAFKNIDNLLDGFENIAIYQYQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5VS06</accession>
    <name>R5VS06_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01118</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000089">
        <property type="protein sequence ID" value="CCZ83337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83337">
        <property type="protein sequence ID" value="CCZ83337"/>
        <property type="gene ID" value="BN566_01118"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="55" mass="6394" checksum="5DE02D0B24CE0CFE" modified="2013-07-24" version="1">MSTMKEILHKKREVTEKHIKQLQQAGKQDVRYTAMMPDIPFLVLGLFSDVGWGDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5VS46</accession>
    <name>R5VS46_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Relaxase/mobilization nuclease family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01143</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000093">
        <property type="protein sequence ID" value="CCZ83367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01143"/>
    <dbReference type="EnsemblBacteria" id="CCZ83367">
        <property type="protein sequence ID" value="CCZ83367"/>
        <property type="gene ID" value="BN566_01143"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005094">
        <property type="entry name" value="Endonuclease_MobA/VirD2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03432">
        <property type="entry name" value="Relaxase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="447"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="326"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="466" mass="54066" checksum="7C6C9E3ACBB6DAFA" modified="2013-07-24" version="1">MAITKLFAIKTTEVKALAYIANPEKTDGGRLIFTYGCSSDPAQASRDFEAVRAGGTGLNTVLSQHFIQSFKPGEITPERALEVGKELCEKFLKGEYQYFLAIHTDTDHTHLHVIFNNVNRKNGRTFETNMNQGKVKERAWKKLIDLSDEVCKRHHLDVIEHPERTKGKSHWEWDMNRQGLSWKAKLKYTIDQVVKASEDFDDFIRKCAEYGVLVEYNPDHKIDLKFMLSEQKERNPRAKMTRARTLGWYYETRQIKDRIAQYHGVMIYTPRTKVRVITKKPPNKFVQDAIDRGNMKLASIAKNVIAEYGAPPDQIRQAAISEYAHSRGLLSELNTMKTEIEDLQVKLKVLRKYRKLKVYGEELKTLSGSAAKKYRREHSAELTEYGQIRTKVLELYPSGHIPTVESLDKKINALIQERSLKDQQFREADKRARDLADAQRTIEEFLRQERNEQQQDCKRKKNGDLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>R5VS57</accession>
    <name>R5VS57_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">ABC-type multidrug transport system ATPase and permease components</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN566_01153</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000093">
        <property type="protein sequence ID" value="CCZ83377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01153"/>
    <dbReference type="EnsemblBacteria" id="CCZ83377">
        <property type="protein sequence ID" value="CCZ83377"/>
        <property type="gene ID" value="BN566_01153"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="59"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="139"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="22"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="333"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="200"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="366"/>
            <end position="373"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ83377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="579" mass="63234" checksum="13B4D41CCC4FB046" modified="2013-07-24" version="1">MMEKIQRKFALSPQGAKDLIKSIICCTLQNIVLMFPVCLLYLLVQDLMNGGVSGTKTGFYIAGIVVCLALICAVTYFQYNAAFLATYVESGVRRIHLSEKLRKIPLSFFGEKDLSDLTSTMMADCATLETAFSHFIPELVGSMISTVLIALSLLFFEWHMALAALWVIPVAFIIIAASSKVQDHLSQKSMQAKMACADGIQEYIETLQDLKANNAEKEYLNGLEQKVRAVEQRSIVSEFGTAAFVVSANMILKLGIATVALVGAILLSKGTIDVLTFFLFLLVASRLYDPLQGALQNLAAIISTRTNVARMNEILDHPTQQGADVLSNQGCDITFSHVGFAYDNGETILKDISFTAKQGAVTALVGPSGSGKTTVSRLAARFWDTKQGTITVGGMDISKVDPETLMTLYSIVFQDVTLFHDTILENIRLGKKGATDEEVLRAAKLANCDEFAEKLPNGYQTMIGENGSALSGGERQRISIARAFLKDAPIILLDEATASLDVENETLIQTALSRLIANKTVLMIAHRMRTVANADKILVLKDGTIVESGTPEELMQKQNGLFRHMTELQKQSSVWEVGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5VS75</accession>
    <name>R5VS75_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Efflux transporter RND family MFP subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01168</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000094">
        <property type="protein sequence ID" value="CCZ83392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01168"/>
    <dbReference type="EnsemblBacteria" id="CCZ83392">
        <property type="protein sequence ID" value="CCZ83392"/>
        <property type="gene ID" value="BN566_01168"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032317">
        <property type="entry name" value="HlyD_D23"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16576">
        <property type="entry name" value="HlyD_D23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HlyD_D23" evidence="3">
        <location>
            <begin position="67"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="383"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="102"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="397"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="433" mass="47437" checksum="C873F557E5526C79" modified="2013-07-24" version="1">MQTSKKIIALVAAVVVLAAAGGGIWLHHRKTAETESAAAVYVSTVAELNAANPDLTTLCYSGVTEAQETKEIKADTSKTIAETYVKEGDTVKKGTPLFLYDVASMQLDLEQGQIEVEQLNNQIASYQEQIQQLESEKANADADQQLSYTTQIQSLQTDIDKAQYDIKVKNIALQKQQNAIDNAKVTAEMDGIVKTVKTPEAMQADGTDVIMTITASGNYRIKGTLSEQQLGMVSTGEKMVIRSRIDPEQYWTGTVTEIGTEAEQNSNNNMMYDSSSSDNTASKYAFYIKLDKADGLLLGQHVLIEPDVGQNSGHTGIWLYQDYLVQDGDNSYVWVSNSKHELEKKSVTLGDTDDDYGIVELKSGLSETDLIAYPADDYKEGMKTTTNANNLSDGETDGNFSDEEPIEEPMYDQEEPMIEETEEAAAEDAASGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5VSN4</accession>
    <name>R5VSN4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ABC-type transport system involved in lipoprotein release permease component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01301</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000121">
        <property type="protein sequence ID" value="CCZ83532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01301"/>
    <dbReference type="EnsemblBacteria" id="CCZ83532">
        <property type="protein sequence ID" value="CCZ83532"/>
        <property type="gene ID" value="BN566_01301"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038766">
        <property type="entry name" value="Membrane_comp_ABC_pdt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30287">
        <property type="entry name" value="PTHR30287"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="362"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="529"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="747"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="801"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="842"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="258"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="877" mass="97256" checksum="76531B7B6D39278E" modified="2013-07-24" version="1">MAMAGLLGKELCRTVRHTFGQFTAIAAIVALGCGFFAGIQATTPDMNEMADQHYRETNLMDLQIRSTIGLDEQEVQAVEQLPDVKTVSAGYQVQCYLPQDVHSYSMSVSSMNMEQAADGAGINLPTLTAGTYPSISTECLMDANFAKRRGYQVGDTITLQAAEDTTLSDYLQEDTFTISGLTNWSMYVSFERGTAQIGTGALDGYLLVDDSAFSMDVYTNLYLTLDSTADLAAQSDAYTTAANDAKAVMEREGTAILKQRVERETADAQEQLTEARSNLETQQAEYAKNFAQLADAYGTEAASQQLADAEQQLNDAEKQIQEQQTALDDFADNAKWYVQTREDNVGYSAFWENTERVQNVVAVFPVFFILIAALVCLTTMTRMVREQRVEMGTKKALGYGAFPIMAKFLLYAGIATLIGGITGLLIGFQVFPRIICNAYATMYYLQDIPCPFRWKSALACLGVGLLCTCLSSAIACRNALREQPAQLMRPKPPKSGKRILLERIPWLWNRIGFMGKLTLRNVFRYKSRFWMTAVGVGGCTALILAGFGLRYDITSIADLQYSEIMQYDLLAVYNDDAEQTRTEALSSWEENPHTMLLQCSEQVGEYDVTLMVTAEPEQLGNFITMRSRSDHTPYTLDDSGVILTEKLAKLLKVSAGDTLQLKDAKKPVRITAVTENYAYHYIYMTENTYQSLYDTERAPNTMLVQLNNPDTADEHATALMQQDGMLTVQSLHRSGNVFSDLIDSMNLIVIVLIVCAGALAVVVLYNLSNINITERMRELATVKVLGFYDGESAAYIYRENFFSMTVGILFGLVGGVFLTRFVVSMAEVDVVMFARTIPPYAFVFAAGLTVLFTLLVNLILYPKINRIDMASALKAIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>R5VTP9</accession>
    <name>R5VTP9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7 8">Elongation factor Tu</fullName>
            <shortName evidence="7">EF-Tu</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tuf</name>
        <name evidence="10" type="ORF">BN566_01626</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000171">
        <property type="protein sequence ID" value="CCZ83897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01626"/>
    <dbReference type="EnsemblBacteria" id="CCZ83897">
        <property type="protein sequence ID" value="CCZ83897"/>
        <property type="gene ID" value="BN566_01626"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01884">
        <property type="entry name" value="EF_Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03697">
        <property type="entry name" value="EFTU_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00118_B">
        <property type="entry name" value="EF_Tu_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041709">
        <property type="entry name" value="EF-Tu_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033720">
        <property type="entry name" value="EFTU_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004541">
        <property type="entry name" value="Transl_elong_EFTu/EF1A_bac/org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004160">
        <property type="entry name" value="Transl_elong_EFTu/EF1A_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03143">
        <property type="entry name" value="GTP_EFTU_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="SSF50465"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00485">
        <property type="entry name" value="EF-Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 7 10" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="5 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="9">
        <location>
            <begin position="10"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="19"/>
            <end position="26"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="81"/>
            <end position="85"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="136"/>
            <end position="139"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ83897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="399" mass="43781" checksum="A267573F48E15EF9" modified="2013-07-24" version="1">MAKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKTLALKGQAQYEAYDMIDKAPEERERGITINTAHVEYETEKRHYAHVDCPGHADYVKNMITGAAQMDGAILVVAASDGPMAQTKEHILLARQVGVPAIVVFMNKADQVDDPELLELVEMDIRETLNEYEFPGDEVPIIVGSALAALEAPDDLSNPAYKPILDLMDAVDEYIPTPERDDAKPFLMPIEDTMTISGRGTVVTGRVERGKLNVNETVEIVGLSPEKLSTVVTGIEMFRKTLDFAEAGDNIGALLRGITRQQVERGQVLCKPGSIHPHTKFKGQVYVLKKEEGGRHTPFFNNYRPQFYFRTTDVTGIITLPADVEMCTPGDNVEMTVELIAPIAIEEGLRFAIREGGRTVGSGVVTSVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>R5VTR0</accession>
    <name>R5VTR0_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase</fullName>
            <ecNumber evidence="10">2.4.1.227</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Undecaprenyl-PP-MurNAc-pentapeptide-UDPGlcNAc GlcNAc transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">murG</name>
        <name evidence="14" type="ORF">BN566_01636</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Cell wall formation. Catalyzes the transfer of a GlcNAc subunit on undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide (lipid intermediate I) to form undecaprenyl-pyrophosphoryl-MurNAc-(pentapeptide)GlcNAc (lipid intermediate II).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>di-trans-octa-cis-undecaprenyl diphospho-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine + UDP-N-acetyl-alpha-D-glucosamine = di-trans-octa-cis-undecaprenyl diphospho-[N-acetyl-alpha-D-glucosaminyl-(1-&gt;4)]-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine + H(+) + UDP</text>
            <dbReference type="Rhea" id="RHEA:31227"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:61387"/>
            <dbReference type="ChEBI" id="CHEBI:61388"/>
            <dbReference type="EC" id="2.4.1.227"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="10">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Peripheral membrane protein</topology>
            <orientation evidence="10">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the glycosyltransferase 28 family. MurG subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.227" evidence="10"/>
    <dbReference type="EMBL" id="CBAR010000172">
        <property type="protein sequence ID" value="CCZ83907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01636"/>
    <dbReference type="EnsemblBacteria" id="CCZ83907">
        <property type="protein sequence ID" value="CCZ83907"/>
        <property type="gene ID" value="BN566_01636"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051991">
        <property type="term" value="F:UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol 4-beta-N-acetylglucosaminlytransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050511">
        <property type="term" value="F:undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030259">
        <property type="term" value="P:lipid glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00033">
        <property type="entry name" value="MurG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006009">
        <property type="entry name" value="GlcNAc_MurG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007235">
        <property type="entry name" value="Glyco_trans_28_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004276">
        <property type="entry name" value="GlycoTrans_28_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04101">
        <property type="entry name" value="Glyco_tran_28_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03033">
        <property type="entry name" value="Glyco_transf_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01133">
        <property type="entry name" value="murG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 10" id="KW-0132">Cell division</keyword>
    <keyword evidence="1 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 10" id="KW-0133">Cell shape</keyword>
    <keyword evidence="9 10" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="3 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_transf_28" evidence="12">
        <location>
            <begin position="3"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_tran_28_C" evidence="13">
        <location>
            <begin position="193"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="UDP-N-acetyl-alpha-D-glucosamine binding" evidence="10">
        <location>
            <begin position="10"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="375"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="377"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CCZ83907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="405" mass="43674" checksum="6FCD23381398C628" modified="2013-07-24" version="1">MKVLFAGGGTGGHINPALAIASIIQSHQPDAEFLFAGTPNGMEAKLVPQSGYQLATIKVAGFQRKLTMKNIRRNAQAAWYLATSGKRAKQIVNEFQPDIAIGTGGYVAGPVIRMAAKMGVPCAIHEQNAFPGVTNKMLSKEVDHVMLTVKEALEYMDFDCPYTITGLPVRAGILQKTKEQARKELGFDDSMCILSFGGSLGAGCINEVMEELIPWHVKNGMAINHIHGYGGMGRESFPAAMRAAGIPMRSDRLRITEYIHDMDTCLAAADLVICRAGASTLAELEAAGKASILIPSPIVAGNHQLHNANVLGKAGAAIVIEQKNVTVEGMMHHVEELYENRAMLKDMAYQASQLAIPDTADRIYRVVETLMEKAGKPLHASADDSTETDSAEVQKEEPKTESKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5VTX1</accession>
    <name>R5VTX1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01696</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000177">
        <property type="protein sequence ID" value="CCZ83967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01696"/>
    <dbReference type="EnsemblBacteria" id="CCZ83967">
        <property type="protein sequence ID" value="CCZ83967"/>
        <property type="gene ID" value="BN566_01696"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014194">
        <property type="entry name" value="Spore_III_AE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09546">
        <property type="entry name" value="Spore_III_AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004395651" evidence="2">
        <location>
            <begin position="30"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="192"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="230"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="263"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="291"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="343"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="373" mass="40450" checksum="03BCA27138EB40E1" modified="2013-07-24" version="1">MSKKTSMMVIVILFAISLLCRWLFVPVQAIELFQSGTVDWVEQLDPDLQEQLESYGISMEHPEELQQFSVGSIFKTVLDGIQKEITAPIRLLLLFLAVILCSALLHAVQHGKNAGICEMMGVMGAVSACSKELCDCFSQGKAILSQCTDFMTLFTPVFSMFMTATGQFGSAAIYQTSSLFLTNSILQILDHILLPFLSMSFAIAIANAVYAEHTLDGLLQFNKTIVTWSLGFLMSVFIGVISIQSIVSSAADGLTTKTTKYVVSNFVPIVGGAVSDAYATVLGSLQVLKSATGAIGIVVLLLIFLPILLRVVLYRLVLKLAVACADLFSVSQVSRFLKGAEQVLSMLFAILTCFAVLFLVTVALLLLISRPNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>R5VTY6</accession>
    <name>R5VTY6_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">UDP-N-acetylmuramoylalanine--D-glutamate ligase</fullName>
            <ecNumber evidence="9">6.3.2.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">D-glutamic acid-adding enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">murD</name>
        <name evidence="12" type="ORF">BN566_01711</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + D-glutamate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine = ADP + H(+) + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate</text>
            <dbReference type="Rhea" id="RHEA:16429"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29986"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83898"/>
            <dbReference type="ChEBI" id="CHEBI:83900"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the MurCDEF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.9" evidence="9"/>
    <dbReference type="EMBL" id="CBAR010000178">
        <property type="protein sequence ID" value="CCZ83982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01711"/>
    <dbReference type="EnsemblBacteria" id="CCZ83982">
        <property type="protein sequence ID" value="CCZ83982"/>
        <property type="gene ID" value="BN566_01711"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008764">
        <property type="term" value="F:UDP-N-acetylmuramoylalanine-D-glutamate ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00639">
        <property type="entry name" value="MurD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005762">
        <property type="entry name" value="MurD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006151">
        <property type="entry name" value="Shikm_DH/Glu-tRNA_Rdtase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01488">
        <property type="entry name" value="Shikimate_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="SSF53244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01087">
        <property type="entry name" value="murD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 9" id="KW-0132">Cell division</keyword>
    <keyword evidence="5 9" id="KW-0133">Cell shape</keyword>
    <keyword evidence="8 9" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 9" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Shikimate_DH" evidence="10">
        <location>
            <begin position="5"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_M" evidence="11">
        <location>
            <begin position="121"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="123"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CCZ83982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="464" mass="51243" checksum="DEDAA8BF7ED9094A" modified="2013-07-24" version="1">MTKIERFFANLKEKKVAFIGTGVTNNDIIRLFLKKGISITLLDRKTPEKLGAIYEELKAAGVAFQLGDTYLDHLTDFDVVFRSPGMYYHNEKLQAARKQGVVITTEMEVFFDLCPCKIYAVTGSDGKTTTTTLISEFLQASGVTVHKGGNIGRALLPIIEQIQDTDAAVVELSSFQLLSMRQSPDVAVITNIAPNHLDVHGTMEEYIQAKTNLIAHQNAFSKTILNADNALTWNLAPMVRGMLVAFTRKTVPAYGAFLREDGMLCYQNGDTCTPVVAASDIRIPGIHNVENYLAAISAVWGEVSTDVIVQVAKTFGGVEHRIEFVRERKGVKWYNDSIATSPTRVLAGLNSFQTKLTVIMGGYDKKIPFEPMAETVCDKVKTVILMGVTAPKIEAAIRGAKNYQEGHPQLLHVETMEEAVQTADQITKPGDIVTLSPACASFDKYPNFEERGLHYKRLVKELSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>R5VUP5</accession>
    <name>R5VUP5_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ribosomal protein S12 methylthiotransferase RimO</fullName>
            <shortName evidence="8">S12 MTTase</shortName>
            <shortName evidence="8">S12 methylthiotransferase</shortName>
            <ecNumber evidence="8">2.8.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Ribosomal protein S12 (aspartate-C(3))-methylthiotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Ribosome maturation factor RimO</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rimO</name>
        <name evidence="12" type="ORF">BN566_00011</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>[sulfur carrier]-SH + AH2 + L-aspartate(89)-[ribosomal protein uS12]-hydrogen + 2 S-adenosyl-L-methionine = 3-methylsulfanyl-L-aspartate(89)-[ribosomal protein uS12]-hydrogen + 5'-deoxyadenosine + [sulfur carrier]-H + A + 2 H(+) + L-methionine + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:37087"/>
            <dbReference type="Rhea" id="RHEA-COMP:10460"/>
            <dbReference type="Rhea" id="RHEA-COMP:10461"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:29961"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="ChEBI" id="CHEBI:73599"/>
            <dbReference type="EC" id="2.8.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="8">Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the methylthiotransferase family. RimO subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.4" evidence="8"/>
    <dbReference type="EMBL" id="CBAR010000197">
        <property type="protein sequence ID" value="CCZ84242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00011"/>
    <dbReference type="EnsemblBacteria" id="CCZ84242">
        <property type="protein sequence ID" value="CCZ84242"/>
        <property type="gene ID" value="BN566_00011"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103039">
        <property type="term" value="F:protein methylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018339">
        <property type="term" value="P:peptidyl-L-beta-methylthioaspartic acid biosynthetic process from peptidyl-aspartic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01865">
        <property type="entry name" value="MTTase_RimO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005839">
        <property type="entry name" value="Methylthiotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020612">
        <property type="entry name" value="Methylthiotransferase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013848">
        <property type="entry name" value="Methylthiotransferase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038135">
        <property type="entry name" value="Methylthiotransferase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005840">
        <property type="entry name" value="Ribosomal_S12_MeSTrfase_RimO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041582">
        <property type="entry name" value="RimO_TRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43837">
        <property type="entry name" value="PTHR43837"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18693">
        <property type="entry name" value="TRAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00919">
        <property type="entry name" value="UPF0004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00274">
        <property type="entry name" value="ribosomal_protein_S12_methylth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00089">
        <property type="entry name" value="TIGR00089"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01125">
        <property type="entry name" value="TIGR01125"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51449">
        <property type="entry name" value="MTTASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01278">
        <property type="entry name" value="MTTASE_RADICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 8" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0408">Iron</keyword>
    <keyword evidence="7 8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="12" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 8" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 8 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MTTase N-terminal" evidence="10">
        <location>
            <begin position="3"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="11">
        <location>
            <begin position="141"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="TRAM" evidence="9">
        <location>
            <begin position="374"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CCZ84242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="444" mass="50244" checksum="12B857ECCA0A9D67" modified="2013-07-24" version="1">MPIRVGMVSLGCSKNLVDSERMLFKLRKHGYTLVKEPGDADVVVVNTCGFIQSAKEEAIETILELAELKAEGTIQKIILTGCLTERYQEEVADQFAEVDAVVGIGDQKDIVSILESVLKKDRVVQFAPKSDAELMGDRIITTLPFYSYLKVAEGCSNRCSYCAIPKIRGPYRSVPMEAVLEEANRLAERGVTELIVIAQDTTQYGKDLYGESRLPQLLKALCEIPTVHWVRVLYCYPERITDELLDVMAAEEKIVKYLDIPIQHCDGEILRRMYRPGNETMLRNLIAKIREKMPDVTLRTTLITGFPGETKEQFNALGDFVQDMQFDRLGCFAYSEEEGTAAAEYEDQIDEETREHRAEILMQQQAELHAEKNAAKIGTQMEAVIEGFDPWAGCYFGRTRADAPEIDGKIFIRRTKEHQCGDYVQVEVFDTMDYDLLAEEVEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VUS8</accession>
    <name>R5VUS8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00206</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000207">
        <property type="protein sequence ID" value="CCZ84277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84277">
        <property type="protein sequence ID" value="CCZ84277"/>
        <property type="gene ID" value="BN566_00206"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="31" mass="3202" checksum="EC6E5F3C25EFD52C" modified="2013-07-24" version="1">MGGLFGLILLCVLVGGGIKLGTDIILNLFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5VUT1</accession>
    <name>R5VUT1_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">TM1586_NiRdase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00021</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000208">
        <property type="protein sequence ID" value="CCZ84282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84282">
        <property type="protein sequence ID" value="CCZ84282"/>
        <property type="gene ID" value="BN566_00021"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.109.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000415">
        <property type="entry name" value="Nitroreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029478">
        <property type="entry name" value="TM1586_NiRdase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14512">
        <property type="entry name" value="TM1586_NiRdase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55469">
        <property type="entry name" value="SSF55469"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TM1586_NiRdase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="223" mass="25133" checksum="CEA06C1D4DA600FB" modified="2013-07-24" version="1">MDLFTAMVERHSVRSYTTQPITDPVKSQLQTFLKQCNQESGLHMQLILDEPQAFDGLLARYGNFKNVRNYIALVGRKSDRLGEQCGYYGEKLVLFAQTLGLRTCWVGASYRKRKAAFQLEDGEKCCLVIAIGYGAEDGVPHHSKSLESVIRIKDEMPHWFEKGVQAALLAPTAVNQQKFLFTLVNDHTVRAGAGWGLYTEVDLGIVKYHFELAAGDHFEWAES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5VUT7</accession>
    <name>R5VUT7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00026</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000208">
        <property type="protein sequence ID" value="CCZ84287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84287">
        <property type="protein sequence ID" value="CCZ84287"/>
        <property type="gene ID" value="BN566_00026"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="115" mass="13487" checksum="872BF44F1364B8D2" modified="2013-07-24" version="1">MHDSLWQFLLLDLLLPCFIVTEVVECLAAWIYPKRNCKDLFLVFLVNVLTNPAAVFINFFVPFRITHPVLWILFFEGLIWLVEALIYRRCLTQKRNPFLFSFVLNAASYGIGLFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5VUX4</accession>
    <name>R5VUX4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00235</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000213">
        <property type="protein sequence ID" value="CCZ84322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00235"/>
    <dbReference type="EnsemblBacteria" id="CCZ84322">
        <property type="protein sequence ID" value="CCZ84322"/>
        <property type="gene ID" value="BN566_00235"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HDc" evidence="1">
        <location>
            <begin position="32"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="222" mass="25401" checksum="ACB9747F936A59B5" modified="2013-07-24" version="1">MASCVTFEYIRKNPDIRTYIQRADEALKSIGYTEHSFPHVEKAAATAARILTELGYPEREIELARIAGFLHDIGNVINRVDHAQSGAVMAFRLLDRLEMPVDEICSVISAIGNHDEATAQPIDAISAALILADKTDVRRSRVRNTDFLTFDIHDRVNYAVETAELLIDKEQQEFVLSMTIDTQISSVLEYFEIFMERMLLCRRAAQFFHMQFKIYINHSLML</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>R5VUZ0</accession>
    <name>R5VUZ0_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Co-chaperonin GroES</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">10 kDa chaperonin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Chaperonin-10</fullName>
            <shortName evidence="3">Cpn10</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">groES</name>
        <name evidence="3" type="synonym">groS</name>
        <name evidence="5" type="ORF">BN566_00250</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 4">Together with the chaperonin GroEL, plays an essential role in assisting protein folding. The GroEL-GroES system forms a nano-cage that allows encapsulation of the non-native substrate proteins and provides a physical environment optimized to promote and accelerate protein folding. GroES binds to the apical surface of the GroEL ring, thereby capping the opening of the GroEL channel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heptamer of 7 subunits arranged in a ring. Interacts with the chaperonin GroEL.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3 4">Belongs to the GroES chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000215">
        <property type="protein sequence ID" value="CCZ84337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00250"/>
    <dbReference type="EnsemblBacteria" id="CCZ84337">
        <property type="protein sequence ID" value="CCZ84337"/>
        <property type="gene ID" value="BN566_00250"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00320">
        <property type="entry name" value="cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.33.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00580">
        <property type="entry name" value="CH10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020818">
        <property type="entry name" value="Chaperonin_GroES"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037124">
        <property type="entry name" value="Chaperonin_GroES_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10772">
        <property type="entry name" value="PTHR10772"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00166">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00297">
        <property type="entry name" value="CHAPERONIN10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00883">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ84337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="96" mass="10086" checksum="B286B18D86CBAA18" modified="2013-07-24" version="1">MLKPLADRVIVKFEEEKEEKTVGGLILTTKAASSSYATATVVACGAGKVASTGTLIPMTVKAGDKVLLSKSAGQKIKDNDEELTVVYESEIIAVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVJ5</accession>
    <name>R5VVJ5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted RNA binding protein (Contains ribosomal protein S1 domain)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00401</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000240">
        <property type="protein sequence ID" value="CCZ84502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00401"/>
    <dbReference type="EnsemblBacteria" id="CCZ84502">
        <property type="protein sequence ID" value="CCZ84502"/>
        <property type="gene ID" value="BN566_00401"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035104">
        <property type="entry name" value="Ribosomal_protein_S1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10724:SF7">
        <property type="entry name" value="PTHR10724:SF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="S1 motif" evidence="2">
        <location>
            <begin position="6"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="162" mass="18903" checksum="CF651F6291F7B230" modified="2013-07-24" version="1">MQLEIGSTYQGKVKRITNYGAFIAIQPDAAAPETVTGMVHISEISNRFVHDIHEFVQEGQEVQVKVIGMQDNKISLSMKQTEQPQQQQRTRNQNGDAPHPRSQQRPPRRDRDRPNVWQPPKPKPPMSELSFEDMLSQFKQNSEERICDIKRNTERKNRTRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5UWQ1</accession>
    <name>R5UWQ1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00008</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000001">
        <property type="protein sequence ID" value="CCZ82753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00008"/>
    <dbReference type="EnsemblBacteria" id="CCZ82753">
        <property type="protein sequence ID" value="CCZ82753"/>
        <property type="gene ID" value="BN566_00008"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003735">
        <property type="entry name" value="Metal_Tscrpt_repr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038390">
        <property type="entry name" value="Metal_Tscrpt_repr_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33677">
        <property type="entry name" value="PTHR33677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02583">
        <property type="entry name" value="Trns_repr_metal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ82753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="91" mass="10114" checksum="5020D43957504855" modified="2013-07-24" version="1">MKQCMDADNLHRRLKKIIGQVQAIDRMIDEDVPCEDILAQINAAKSALHGCGKVVLEGHIKHCVRDGIQHGDADKTIESFTKAVERFSNMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>R5UWY9</accession>
    <name>R5UWY9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7 9">GTPase Der</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">GTP-binding protein EngA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">der</name>
        <name evidence="11" type="ORF">BN566_00638</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 9">GTPase that plays an essential role in the late steps of ribosome biogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Associates with the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8 9">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. EngA (Der) GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000012">
        <property type="protein sequence ID" value="CCZ82833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00638"/>
    <dbReference type="EnsemblBacteria" id="CCZ82833">
        <property type="protein sequence ID" value="CCZ82833"/>
        <property type="gene ID" value="BN566_00638"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00195">
        <property type="entry name" value="GTPase_Der"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031166">
        <property type="entry name" value="G_ENGA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016484">
        <property type="entry name" value="GTP-bd_EngA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032859">
        <property type="entry name" value="KH_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14714">
        <property type="entry name" value="KH_dom-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006485">
        <property type="entry name" value="GTP-binding_EngA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03594">
        <property type="entry name" value="GTPase_EngA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51712">
        <property type="entry name" value="G_ENGA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5 7 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 7" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="EngA-type G" evidence="10">
        <location>
            <begin position="6"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="EngA-type G" evidence="10">
        <location>
            <begin position="180"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="12"/>
            <end position="19"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="59"/>
            <end position="63"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="122"/>
            <end position="125"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="186"/>
            <end position="193"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="233"/>
            <end position="237"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="298"/>
            <end position="301"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ82833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="445" mass="49559" checksum="D9E0B5B66EC67633" modified="2013-07-24" version="1">MPVALPIVAVVGRPNVGKSTLFNKLIGKRLSIVENTPGVTRDRIYAKCEWRDRAFMIVDTGGIEPTTEDVILKQMREQAQLAIEQADVIILVTDVRCGVTADDSAVANMLQKSGKPIILAVNKCDKIGEPPMELYEFYNLGLGDPFPVSAEHGHGSGDMLDAVYDALPPESELEEQDDAIRVAIIGKPNVGKSSLVNRIAGEQRVIVSNVAGTTRDATDTTVENEEGKFVLIDTAGIRRKSKVTEKIEQYSVLRAYMAVDRADVCLIVIDAMEGFTEQDSKVAGYAHEQGKASIVVVNKWDLVEKDGKTMQNFEEELRGHFSFMSYVPFLFLSAKTGQRVSKLFGMIKDVYQQNSIRVTTGTLNDILAYATTRVQPPSDKGRRLKIYYMTQPSTNPPTFVVFVNRADLFHFSYQRYLENQIRASFGLTGTPVRFVVRERNRDDQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXB4</accession>
    <name>R5UXB4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00743</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000029">
        <property type="protein sequence ID" value="CCZ82943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00743"/>
    <dbReference type="EnsemblBacteria" id="CCZ82943">
        <property type="protein sequence ID" value="CCZ82943"/>
        <property type="gene ID" value="BN566_00743"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="SSF55008"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMA" evidence="1">
        <location>
            <begin position="3"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="84" mass="9420" checksum="8BFA641AED21B198" modified="2013-07-24" version="1">MVKTVLKIDGMMCGMCESHMNNLIRNLFPVKKVTSSAKDGETVIISEQELDMSKLETEVAGIGYKLLSYTSEPYVKKGLFSFHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXF4</accession>
    <name>R5UXF4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00778</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the P(II) protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000034">
        <property type="protein sequence ID" value="CCZ82978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00778"/>
    <dbReference type="EnsemblBacteria" id="CCZ82978">
        <property type="protein sequence ID" value="CCZ82978"/>
        <property type="gene ID" value="BN566_00778"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006808">
        <property type="term" value="P:regulation of nitrogen utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002187">
        <property type="entry name" value="N-reg_PII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017918">
        <property type="entry name" value="N-reg_PII_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30115">
        <property type="entry name" value="PTHR30115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00543">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00340">
        <property type="entry name" value="PIIGLNB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00938">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="SSF54913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00638">
        <property type="entry name" value="PII_GLNB_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51343">
        <property type="entry name" value="PII_GLNB_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="O-UMP-tyrosine" evidence="1">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR602187-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ82978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="114" mass="12673" checksum="417DBE972E9E5711" modified="2013-07-24" version="1">MKLSKIEIITGMSKLTGLKSVLSRAGVRGMTVVQVLGCGVEKGTKEYEVDENYEMELLPKQQISIVVKTEKVKEIVELIKQELYTGHIGDGKIFVYDITNVIRVRTGDEGEDAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXG6</accession>
    <name>R5UXG6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00565</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000004">
        <property type="protein sequence ID" value="CCZ82760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82760">
        <property type="protein sequence ID" value="CCZ82760"/>
        <property type="gene ID" value="BN566_00565"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004406522" evidence="2">
        <location>
            <begin position="25"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ82760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="230" mass="24327" checksum="B16B9EF00DCCF10F" modified="2013-07-24" version="1">MKKVLAFLSAIVLAAGVVGCGSDADSSSSSSEAATTEATTEAVTEAETEAETEAETTEAETTETTTGSDDAEADAETSETEEVDLEALAAELTQEETVDNLTLKVSPDWTKSETQGYPMWYLSDMTGALFVQQFDASSMGVSLDDHKKALESVGAAMDAQATVVDTEWDTLNGEDAYGVVYTMDVSDITTTNMSVFFFVGDTICGITFTDFGGTGTIMNYAQPILDSITF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXM0</accession>
    <name>R5UXM0_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Hydrolase_4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00821</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000040">
        <property type="protein sequence ID" value="CCZ83028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00821"/>
    <dbReference type="EnsemblBacteria" id="CCZ83028">
        <property type="protein sequence ID" value="CCZ83028"/>
        <property type="gene ID" value="BN566_00821"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydrolase_4" evidence="1">
        <location>
            <begin position="33"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="312" mass="35368" checksum="5D044DD7B21B2CA8" modified="2013-07-24" version="1">MLPTTIQPFTLLSDQDGLPLSGLLLLPQEETTPCGILQVSHGMCEHKERYLDFLRHCTKQGYICLIHDHRGHGESISDTIPLGYFGEGGAEALVADLHQMTEWIQTQYAGLPLYLLGHSMGSLIARCYLRQYPDILDGLILSGSPSPLPAMTRMQSILNRVIVHRGDTSHSKRIDRILYNTFQRPFRSESRSNTWICSDEAVVDAYNADPLCQFHFTLNGYAALLALLNTTYNTKDWTITQPELPIWFFSGGDDPCRGNDTRFQQAVDRMAELGYQNVNSTLFPRMRHEILNERNKAVVYAEILETLDAWNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXQ8</accession>
    <name>R5UXQ8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ion-translocating oxidoreductase complex subunit D</fullName>
            <ecNumber evidence="10">7.-.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Rnf electron transport complex subunit D</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">rnfD</name>
        <name evidence="11" type="ORF">BN566_00881</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Part of a membrane-bound complex that couples electron transfer with translocation of ions across the membrane.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="10">The complex is composed of six subunits: RnfA, RnfB, RnfC, RnfD, RnfE and RnfG.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the NqrB/RnfD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.-.-.-" evidence="10"/>
    <dbReference type="EMBL" id="CBAR010000054">
        <property type="protein sequence ID" value="CCZ83093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00881"/>
    <dbReference type="EnsemblBacteria" id="CCZ83093">
        <property type="protein sequence ID" value="CCZ83093"/>
        <property type="gene ID" value="BN566_00881"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022900">
        <property type="term" value="P:electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00462">
        <property type="entry name" value="RsxD_RnfD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004338">
        <property type="entry name" value="NqrB/RnfD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011303">
        <property type="entry name" value="RnfD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30578">
        <property type="entry name" value="PTHR30578"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30578:SF0">
        <property type="entry name" value="PTHR30578:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03116">
        <property type="entry name" value="NQR2_RnfD_RnfE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01946">
        <property type="entry name" value="rnfD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="3 10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4 10" id="KW-0288">FMN</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="94"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="184"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="220"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="244"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="modified residue" description="FMN phosphoryl threonine" evidence="10">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ83093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="332" mass="34907" checksum="067A6CE81C00CE65" modified="2013-07-24" version="1">MQLLTVSPSPHVRSGMTTQKIMGLVLLALLPAAVCGVIFFGIRALLLMLFCILISMATEAICQKLMKKPVTISDGSAALTGLLLALNLPATLPLWKAAVGCIFAIAVVKQLFGGLGCNFANPAITGRVFLLLSFAGDMTTWVKPFYYHGLSIGNQLSSSAGSLDLDAVTSATPLVTGTESLSNLFLGNVGGCIGETSALALLLGGLFLIFTRVISPSTPIAYLGSLGILTFFHALATGGSLTEVPYALLSGGVMIGAFFMATDYVTTPITTKGKWIFGIGCGILTFVIREFASMPEGCSFSILLMNLLTPYIDKFTMTKPLGAKPIKQKEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXR9</accession>
    <name>R5UXR9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cobalamin synthesis G-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00677</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000020">
        <property type="protein sequence ID" value="CCZ82875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00677"/>
    <dbReference type="EnsemblBacteria" id="CCZ82875">
        <property type="protein sequence ID" value="CCZ82875"/>
        <property type="gene ID" value="BN566_00677"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021745">
        <property type="entry name" value="CbiG_mid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021744">
        <property type="entry name" value="CbiG_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002750">
        <property type="entry name" value="CobE/GbiG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036518">
        <property type="entry name" value="CobE/GbiG_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038029">
        <property type="entry name" value="GbiG_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01890">
        <property type="entry name" value="CbiG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11761">
        <property type="entry name" value="CbiG_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11760">
        <property type="entry name" value="CbiG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159664">
        <property type="entry name" value="SSF159664"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159672">
        <property type="entry name" value="SSF159672"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CbiG_N" evidence="2">
        <location>
            <begin position="37"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="CbiG_mid" evidence="3">
        <location>
            <begin position="122"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="CbiG_C" evidence="1">
        <location>
            <begin position="205"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF11761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ82875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="332" mass="36157" checksum="0C4909292D0DB6C4" modified="2013-07-24" version="1">MTYYYYLTAAGKQIAEPLQQQFGGILRGKETFTDAILAQDFQAADLLVFVLATGIAVRKLAPLLRTKCTDPAVLVVSQDQRYVISLLSGHLGGANDWTRKIAAFLHAEPVITTATDLQGQLAFDEVAKNNHLAIENISVLKQISGALLNGKSIWLQSDLPVTGTEQYPQISFSEQANSAGKVVISDRTQTRLETLPTLYLRPKDLVIGVGCKKSIPFVHLETCFLSFLETYQYACDSIKAMATISRKQAEPAILQLCETYHIPLQIVPDADIRACGNLFEASAFVQQVTGLPSVSEACSYLAAGKGTILTRKVKYAGVTLAASRCELQPIVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXV9</accession>
    <name>R5UXV9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Threonine--tRNA ligase</fullName>
            <ecNumber evidence="10">6.1.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Threonyl-tRNA synthetase</fullName>
            <shortName evidence="10">ThrRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">thrS</name>
        <name evidence="13" type="ORF">BN566_00119</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + H(+) + L-threonyl-tRNA(Thr)</text>
            <dbReference type="Rhea" id="RHEA:24624"/>
            <dbReference type="Rhea" id="RHEA-COMP:9670"/>
            <dbReference type="Rhea" id="RHEA-COMP:9704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78534"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="10">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.3" evidence="10"/>
    <dbReference type="EMBL" id="CBAR010000068">
        <property type="protein sequence ID" value="CCZ83158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00119"/>
    <dbReference type="EnsemblBacteria" id="CCZ83158">
        <property type="protein sequence ID" value="CCZ83158"/>
        <property type="gene ID" value="BN566_00119"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004829">
        <property type="term" value="F:threonine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006435">
        <property type="term" value="P:threonyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00184">
        <property type="entry name" value="Thr_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002320">
        <property type="entry name" value="Thr-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451">
        <property type="entry name" value="PTHR11451"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01047">
        <property type="entry name" value="TRNASYNTHTHR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="SSF55186"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="SSF81271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00418">
        <property type="entry name" value="thrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 10 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 10" id="KW-0820">tRNA-binding</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="TGS" evidence="12">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="AA_TRNA_LIGASE_II" evidence="11">
        <location>
            <begin position="230"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="339"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="390"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="522"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CCZ83158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="648" mass="74183" checksum="CC7D884E6EC3AA6C" modified="2013-07-24" version="1">MSQLQLKDGSFREFTPGQSAAEIVKGIGMGLYKAACCVKINGEVCDLRTPITEDCTFEVCTFDDPDGKKTFWHTASHVLAQAVKRLYPNAKLAIGPAIDSGFYYDFDVEKPFTAEELRKIEAEMKKIVKENAELEQFTLSPDEAISFLKEQDEPYKVELCEEHAGKGEPISFYRQGEFTDLCAGPHLMKTGVIKAFKLLSCTGAYWRGSEKNKMLSRVYAIAFPKASMLDEHLKQLEEAKMRDHNKLGRELEYFTTVDVVGQGLPILLPKGARVVQLLQRWVEDVEQSKGCLLTKTPLMAKREFYKISGHWDHYLDGMFILGDPYDETKECFALRPMTCPFQYQVYLNRARSYRDLPMRLVETSTLFRNEDSGEMHGLIRVRQFTISEGHYILRPDQLEEEFKGCLELAKYFLGTVGLLDDCTFRFSQWDPANPNNKYEGTKEQWEEAQRVMGNILDHLGLDYTIGIDEAAFYGPKLDIQYKNVYGKEDTLVTIQIDMLLAQRFGMYYTDTDGQKKLPYIIHRTSLGCYERTLAYMIEHFAGAMPLWIAPEQVRLIPIADRHLDYTYEVLHQLQAAGLRCEVDSRAEKVGYKIRQAVLEKVPYMITIGDNEVEQKTVAVRSRKNGDLGSMQLDDLLAKLKEEVDTKAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5UY21</accession>
    <name>R5UY21_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00760</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000032">
        <property type="protein sequence ID" value="CCZ82960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82960">
        <property type="protein sequence ID" value="CCZ82960"/>
        <property type="gene ID" value="BN566_00760"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ82960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="294" mass="34440" checksum="D479C7F79371C4BF" modified="2013-07-24" version="1">MLSKVWEIIQTYSDLMGFELPLLDGLHSSVPMKKNTHLGFDIVNNKIENVKYYFTSDIDIPEKYLQLSVPRQEYSIALADHTASLRWNCRPDFEPAPYTNHRNILHLLLNQIQLQEQEAHCLKIADDIQMSTQTKRYPLVGYGASHDVQDHKKITDIKLYYTFWTIENWDDSYGRNYSDFSKRALQLISSQMKNDLFTQNYLPISDIMLKHDNLISGFACNIGKQETAYKIYYITEDQTWLESVNAMHIFLFGKSLDLRFEKLFEAIRSLGYRYEEIVYAVSGDCQGVKVYFMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5UY46</accession>
    <name>R5UY46_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00780</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000034">
        <property type="protein sequence ID" value="CCZ82980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82980">
        <property type="protein sequence ID" value="CCZ82980"/>
        <property type="gene ID" value="BN566_00780"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="221" mass="25616" checksum="83F179C223D171BE" modified="2013-07-24" version="1">MTNQLFQWEHFQLGEGFVSITENPIHGTDFIIENHFDVCKGEWCIFIAAIMLAICFISLGRIVRSKTTNDGLLFLTKSVDNFFFAAYRVFQRFAVDEIHRFCNVLNRDSRHQTPSANFQKIFPQLLFQFSTGDSCGVYFSAIQVYFRILFLHDNIKNSANIQFHMLPFGRNVQCSCGIYAIIHSLVPFVQIACLSNHFRNLLPDILFRISIISFFCHGYKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5UY60</accession>
    <name>R5UY60_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00783</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000036">
        <property type="protein sequence ID" value="CCZ82990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00783"/>
    <dbReference type="EnsemblBacteria" id="CCZ82990">
        <property type="protein sequence ID" value="CCZ82990"/>
        <property type="gene ID" value="BN566_00783"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030001">
        <property type="term" value="P:metal ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006127">
        <property type="entry name" value="ZnuA-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01297">
        <property type="entry name" value="ZnuA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004394615" evidence="2">
        <location>
            <begin position="27"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ82990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="328" mass="36948" checksum="B6486B18BD936F5A" modified="2013-07-24" version="1">MKWKRFFSCLLCGVLLLCGCTPAANSATTSHQLELVTTLFCYYDFARAILKDTDTIHLRLLLSPGMESHSFEPAPSDILAIQNADLFLYNGGHMEEWVSQVLDSDEQSHPDRIVRCMMDYAPLLEEVELEGSESEHDHEHDHDDHDHDHEGESEMEYDEHIWTSPVIAKDLLYVTRDAIIQADPANAATYQKNADAYAAQLDALNESFTDYFAEPEYRTLLFADKFPLQYFANTYGLHCYAAFSGCSSDTEPSIATISNLMHLVESDHLSKIYYLEVSSPAVAKVIGEQTGATPTVFQSCHTLTQTDFDNGETYVSLMQRNLKALESQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5UY99</accession>
    <name>R5UY99_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00793</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000036">
        <property type="protein sequence ID" value="CCZ83000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00793"/>
    <dbReference type="EnsemblBacteria" id="CCZ83000">
        <property type="protein sequence ID" value="CCZ83000"/>
        <property type="gene ID" value="BN566_00793"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004067">
        <property type="term" value="F:asparaginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036152">
        <property type="entry name" value="Asp/glu_Ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006034">
        <property type="entry name" value="Asparaginase/glutaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027473">
        <property type="entry name" value="L-asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027474">
        <property type="entry name" value="L-asparaginase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037152">
        <property type="entry name" value="L-asparaginase_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00710">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001220">
        <property type="entry name" value="L-ASNase_gatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00139">
        <property type="entry name" value="ASNGLNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00870">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53774">
        <property type="entry name" value="SSF53774"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51732">
        <property type="entry name" value="ASN_GLN_ASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Asparaginase" evidence="2">
        <location>
            <begin position="3"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="active site" description="O-isoaspartyl threonine intermediate" evidence="1">
        <location>
            <position position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR001220-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="321" mass="35216" checksum="C06118E037A8CC86" modified="2013-07-24" version="1">MKLGVIFTGGTIGCQVAEDGYLQLPDSSTYFLLEGAKKRGILDGITVIPTEPFRLFSEQMTGETLSLLVNHVRERICKEDCDGWILLHGSDTLAYTAAVLGYALGTDCSPLVLVASNEPLSNPKANGWTNFAYAVRFLQEQQATGVFVSHCNTDNIPTIHESIQLLQQLPGSGNLYSFRDAVYGTYDKQGQFHQNRTISSRTPLLPFSEPLNLLQPAPLLQLTAGVGGSLPEIPDYIQAIFYACYHSGTICTDASFQAFAAKAADRQIPVYLTGCRSDTLDYDTVKQWQALQVQPLYDIAPIAAYCKSWIQVSIGKSEKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYB0</accession>
    <name>R5UYB0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">FeS assembly protein SufB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01094</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0051 (ycf24) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01094"/>
    <dbReference type="EnsemblBacteria" id="CCZ83313">
        <property type="protein sequence ID" value="CCZ83313"/>
        <property type="gene ID" value="BN566_01094"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010231">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000825">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037284">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045595">
        <property type="entry name" value="SufBD_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01458">
        <property type="entry name" value="SUFBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19295">
        <property type="entry name" value="SufBD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101960">
        <property type="entry name" value="SSF101960"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01980">
        <property type="entry name" value="sufB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SufBD_N" evidence="2">
        <location>
            <begin position="48"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="469" mass="51936" checksum="09807B22E35D6A66" modified="2013-07-24" version="1">MKEEKQQIADIDRSVYDIKDREDDFYKIDAGLTPALVEQISKEKNDPEWMKEFRLHSLEIYNKLQVPDWGPSIEGLHMDQIVTYVRPNTKMKGDWSEVPEDIKNTFERLGIPQAERKSLAGVGAQYDSELVYHNVREEVAAMGVVYTDLESAMHGEYAEMIQKHFMKLVPPTDHKFAALHGAVWSGGSFVYVPKNVTVTIPLQSYFRLNAKGAGQFEHTLIIVEEGANLHFIEGCSAPKYNVANLHAGCVELYVGKNATLRYSTIENWSKNMYNLNTKRALVEEGGKIEWVSGSFGSHVSYLYPMSILKGKGAKMEFTGITFAGNGQDLDTGAKVVHAAPDTSSYINTKSISKDGGVSTFRSAVTVQKEAVGSKSSVSCESLMLDSISRSDTIPAIDVRTGKADVGHEAQIGKISNEAIFYLMSRGLSEEDARACIVSGFADNVSKELPLEYAIEMNNLIRLEMKGSIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYG9</accession>
    <name>R5UYG9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">Orotate phosphoribosyltransferase</fullName>
            <shortName evidence="4">OPRT</shortName>
            <shortName evidence="4">OPRTase</shortName>
            <ecNumber evidence="2 4">2.4.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">pyrE</name>
        <name evidence="6" type="ORF">BN566_00858</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>diphosphate + orotidine 5'-phosphate = 5-phospho-alpha-D-ribose 1-diphosphate + orotate</text>
            <dbReference type="Rhea" id="RHEA:10380"/>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57538"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1 4">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.10" evidence="2 4"/>
    <dbReference type="EMBL" id="CBAR010000051">
        <property type="protein sequence ID" value="CCZ83070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00858"/>
    <dbReference type="EnsemblBacteria" id="CCZ83070">
        <property type="protein sequence ID" value="CCZ83070"/>
        <property type="gene ID" value="BN566_00858"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00119"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004588">
        <property type="term" value="F:orotate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01208">
        <property type="entry name" value="PyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023031">
        <property type="entry name" value="OPRT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004467">
        <property type="entry name" value="Or_phspho_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00336">
        <property type="entry name" value="pyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 4" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pribosyltran" evidence="5">
        <location>
            <begin position="37"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="5-phosphoribose 1-diphosphate binding" evidence="4">
        <location>
            <begin position="71"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="5-phosphoribose 1-diphosphate binding" evidence="4">
        <location>
            <begin position="125"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>orotate</name>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>orotate</name>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ83070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="219" mass="23985" checksum="7FF5C9CDD342D5A2" modified="2013-07-24" version="1">MTYKEEFIRFMVDCGVLTFGSFTLKSGRQAPYFINTGNYKTGKQLAQLGKYYAACMKEHGLNPDTLFGPAYKGIPLATSAAVALATEYDQEVGYCFDRKEVKDHGEGGMFVGKKLANGDKVVIIEDVMTSGKAMAEVYPKLTGAAKVDIIGMVITVDRMEKALQGNQSAVQTVYETYGVPVYAIVNMEDIIAAIQNDVIPGKEYLDAMLAYREQYGVTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZ59</accession>
    <name>R5UZ59_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Arginine decarboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01371</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000144">
        <property type="protein sequence ID" value="CCZ83608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01371"/>
    <dbReference type="EnsemblBacteria" id="CCZ83608">
        <property type="protein sequence ID" value="CCZ83608"/>
        <property type="gene ID" value="BN566_01371"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008286">
        <property type="entry name" value="Prn/Lys/Arg_de-COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036633">
        <property type="entry name" value="Prn/Lys/Arg_de-COase_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03711">
        <property type="entry name" value="OKR_DC_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55904">
        <property type="entry name" value="SSF55904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OKR_DC_1_C" evidence="2">
        <location>
            <begin position="11"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="79" mass="8608" checksum="B929C60BDA9AF6D9" modified="2013-07-24" version="1">MALPPQQAFFAEKEAVPLRFSIGRVCAEFVMCYPPGIPILAPGERITEAIVSYILYAKEKGCSLTGSQDMTLSSLQVVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZA5</accession>
    <name>R5UZA5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01404</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000148">
        <property type="protein sequence ID" value="CCZ83668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83668">
        <property type="protein sequence ID" value="CCZ83668"/>
        <property type="gene ID" value="BN566_01404"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="97" mass="11655" checksum="ABF7D2704222C63B" modified="2013-07-24" version="1">MKNPALQRKNLYNKNEVEYSHRMAIYQGMAMVFVALEWHYGWKEKRLQRLFDNVQSIAEMPPIFGKSPDALEQMQRFKQDYQIDFTKIKLQVKAKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZH6</accession>
    <name>R5UZH6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MATE efflux family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00128</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000101">
        <property type="protein sequence ID" value="CCZ83420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83420">
        <property type="protein sequence ID" value="CCZ83420"/>
        <property type="gene ID" value="BN566_00128"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="168"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="198" mass="22272" checksum="18CE25C9358E5DF0" modified="2013-07-24" version="1">MQGLYTVYILALNIILAGFSDAAVTVLGLYYKLQSFFFIPLFGLQTCIVPVISFNLARKNYSRCKRTMNLSFLISALFMVLGVICFVFFPRQMVDIFSNSQEVHDIGAQAFPIIGASFLPAVFSLMMPVFFQAIGAGVRSIFLSLLRQIVCLVPIFWAFSFLGLQYTWLSFPISECISGGIGLLFYGYVLRQWKHSTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZJ0</accession>
    <name>R5UZJ0_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Leucine--tRNA ligase</fullName>
            <ecNumber evidence="9">6.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Leucyl-tRNA synthetase</fullName>
            <shortName evidence="9">LeuRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">leuS</name>
        <name evidence="15" type="ORF">BN566_01503</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu)</text>
            <dbReference type="Rhea" id="RHEA:11688"/>
            <dbReference type="Rhea" id="RHEA-COMP:9613"/>
            <dbReference type="Rhea" id="RHEA-COMP:9622"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78494"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9 10">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.4" evidence="9"/>
    <dbReference type="EMBL" id="CBAR010000161">
        <property type="protein sequence ID" value="CCZ83768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01503"/>
    <dbReference type="EnsemblBacteria" id="CCZ83768">
        <property type="protein sequence ID" value="CCZ83768"/>
        <property type="gene ID" value="BN566_01503"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004823">
        <property type="term" value="F:leucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006429">
        <property type="term" value="P:leucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00049_B">
        <property type="entry name" value="Leu_tRNA_synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002302">
        <property type="entry name" value="Leu-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025709">
        <property type="entry name" value="Leu_tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015413">
        <property type="entry name" value="Methionyl/Leucyl_tRNA_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43740">
        <property type="entry name" value="PTHR43740"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13603">
        <property type="entry name" value="tRNA-synt_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09334">
        <property type="entry name" value="tRNA-synt_1g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00985">
        <property type="entry name" value="TRNASYNTHLEU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="SSF50677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00396">
        <property type="entry name" value="leuS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="tRNA-synt_1g" evidence="13">
        <location>
            <begin position="40"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA-synt_1_2" evidence="14">
        <location>
            <begin position="220"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA-synt_1" evidence="11">
        <location>
            <begin position="416"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="domain" description="Anticodon_1" evidence="12">
        <location>
            <begin position="657"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="9">
        <location>
            <begin position="586"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="589"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF09334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF13603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CCZ83768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="807" mass="91928" checksum="4402550D0AB60BA5" modified="2013-07-24" version="1">MSKYDFTAIEAKWQRYWAEHDTFHAEDDYTKPKFYALVEFPYPSGQGLHIGHPRPYTAMDVVSRKRRMEGYNVLFPMGWDAFGLPTENYAIKHKIHPAIVTANNVARFKSQLQALGMSFDWDREINTTDPNYFHWTQWIFLQMFKKGLAYKKETAVNWCTSCKVVLANEEVVGGVCERCGGEVVQKVKSQWMLKITEYAQRLLDDLDEVDYLEKIKTAQRNWIGRSTGAEVDFQTTAGDTLKIYTTRPDTLFGATYMVISPEHPLIEKWADKLENMDEIRAYQEKAARKSDFERTEMAKEKTGVCLKGVRGINPVNDKEIPIFVSDYVLMSYGTGAIMAVPAHDTRDYEFAKVFDLPIIEVVKGGDVTKEAFTDCATGTMVNSGFLDGMSVEDAKVKIKGWLEATGKGKSKVNYKLRDWVFSRQRYWGEPIPVVYCDKCGWVGLPESELPLTLPDVESYMPTDTGESPLSTLESFINTTCPKCGGAAKRETDTMPQWAGSSWYYLRYCDPKNPNGIASKEALNYWMPVDWYNGGMEHTTLHLLYSRFWHKFLYDIGVVNTKEPYKRRTSHGMILGEDPDHPGKFTKMSKSKGNVINPDDVIKEYGADTLRLYEMFVGDFEKAAPWSTSSIRGCKRFLDRIWALQDNLIDGDTYRESLVRKMHQTIQKVSNDIETLKFNTAIAAMMELLNEITATGSINKAEFRTLLILLNPFAPHITEELYETYCGGILHEQSWPTYDEALCKEDTIEIVVQINGKVRCKMNIAVGAEKDAVLEQALAEPKIAEAVAGKQLVKQIYVPNKLVNFVAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZK9</accession>
    <name>R5UZK9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ATP-dependent zinc metalloprotease FtsH</fullName>
            <ecNumber evidence="7">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ftsH</name>
        <name evidence="10" type="ORF">BN566_01224</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
            <orientation evidence="7">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">In the central section; belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="7"/>
    <dbReference type="EMBL" id="CBAR010000106">
        <property type="protein sequence ID" value="CCZ83450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01224"/>
    <dbReference type="EnsemblBacteria" id="CCZ83450">
        <property type="protein sequence ID" value="CCZ83450"/>
        <property type="gene ID" value="BN566_01224"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01458">
        <property type="entry name" value="FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005936">
        <property type="entry name" value="FtsH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011546">
        <property type="entry name" value="Pept_M41_FtsH_extracell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06480">
        <property type="entry name" value="FtsH_ext"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="SSF140990"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01241">
        <property type="entry name" value="FtsH_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 7 10" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 7 10" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="108"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="AAA" evidence="9">
        <location>
            <begin position="196"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="428"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="204"/>
            <end position="211"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="427"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="431"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="504"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ83450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="618" mass="68437" checksum="C71053BD3BFDA870" modified="2013-07-24" version="1">MEERKPDKKPIIWYWAIAIVIFLLLNMFVFPRVMQHNVKEVGYDTFISMMENKEIGSVEVESSQITFTNKDETEYYKTGVLDDPDLVNRLYESGAKFQSEIVSTVSPITYFLISYILPILLIFLVFRLFTKKMGDRAGGNSMMFGMGKSNAKIYVKSTTGIKFKDVAGEEEAKELLTEIVDFLHKPERYQEIGASMPKGALLVGPPGTGKTLLAKAVAGEADVPFFSISGSEFVEMFVGMGAAKVRDLFKQAAEKAPCIVFIDEIDAIGKKRESGQFGGGNDEREQTLNQLLTEMDGFDGSKGVVILAATNRPESLDPALLRPGRFDRRIPVELPDLQGRVEILKVHAKKIKIAPNVDFQAIARTAAGASGAELANIVNEAALRAVRMGRKSATQEDMMESVEVVIAGYQKKSRVLSNKEKLIVSYHEVGHALVAALQSQSAPVQKITIIPRTSGALGYTMQVEEGERFLMSRTELEHKIATLTGGRVAEDLVFHEITTGASNDIEQATKLARAMISQYGMCDAFGMITLETNGSQYLGGNTNLMCSPDTQRQIDQTAVRIIQEQYERAKAMLQQHMPKLHEIAKFLYDHETITGEEFMNILTQPTAELPNQQAEGTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZL5</accession>
    <name>R5UZL5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01229</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000109">
        <property type="protein sequence ID" value="CCZ83455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01229"/>
    <dbReference type="EnsemblBacteria" id="CCZ83455">
        <property type="protein sequence ID" value="CCZ83455"/>
        <property type="gene ID" value="BN566_01229"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="203"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="293" mass="32941" checksum="627E127320BD236F" modified="2013-07-24" version="1">MDFTQQNQDALLQELNQFTRRTHTLDEVYLLDVLLCDNEVDRDFERFSDSALETLKTLFVGKTGIFDHDPSGKNQTARIFSTRLETHPEQLTSTGVPYTCLKANAYMVRTAGNADFIREIDAGIKKEVSISCSARSHTCSICGKDRRLTGCPHVQGELYQGMLCHTILSDITDAYEWSFVAVPAQRNAGVTKQYGGSADHDRITVLQKQLQSQQAQLSAAEEDIRKELVTLCYLSGQPLQKSLLQAAERMTFPELLDLRQQLRNEVRSTGNSQLQPAEEKAKTAASLQQFQMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZR2</accession>
    <name>R5UZR2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01259</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000117">
        <property type="protein sequence ID" value="CCZ83490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83490">
        <property type="protein sequence ID" value="CCZ83490"/>
        <property type="gene ID" value="BN566_01259"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="231" mass="26198" checksum="FB6AE66B649962CD" modified="2013-07-24" version="1">MLENGYVTLPRLFFRNWEWSDDANTVAVFVRLLIEADIVPNSWHGIPVPRGGMVSSYARLAEKTGLSVKKVRTAIKHLEAAGCVAKTAYPNCTVFAIKNFDLFQAGASLRSNEGQTMDKPTASNGQQNKKIEEQEERNLSILDATSKKIPTLIEIQTFAEQEGLCVDVQKFYDYYTETGWMTKNQQPITNWKSTLLYWNQSEGTPHKKTKQEEPRSENADIYESLIGRVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZR4</accession>
    <name>R5UZR4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cell division protein FtsI/penicillin-binding protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_01578</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transpeptidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000166">
        <property type="protein sequence ID" value="CCZ83843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01578"/>
    <dbReference type="EnsemblBacteria" id="CCZ83843">
        <property type="protein sequence ID" value="CCZ83843"/>
        <property type="gene ID" value="BN566_01578"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008658">
        <property type="term" value="F:penicillin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005311">
        <property type="entry name" value="PBP_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036138">
        <property type="entry name" value="PBP_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001460">
        <property type="entry name" value="PCN-bd_Tpept"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03717">
        <property type="entry name" value="PBP_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00905">
        <property type="entry name" value="Transpeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56519">
        <property type="entry name" value="SSF56519"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PBP_dimer" evidence="4">
        <location>
            <begin position="54"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="Transpeptidase" evidence="3">
        <location>
            <begin position="345"/>
            <end position="646"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="678" mass="74543" checksum="C807AE6CDA83DD2C" modified="2013-07-24" version="1">MKKPISVYVKAAGATLLVLSSACLCGYRLMKIQIVDSNTYISQHYSTDTYTQTIAATRGEIVDCNGDPIVQNSVGYNIIIEPDSFPKDNESGNEVLLQLLHLLADVDVTFEESLPISKTAPFTFTTDDQDTLDEIRTTLNLNLYATAENCMDRLIDEYEIADTYTEEEKRMIAGIRYEMQLRGFSMSNQFTLVKDIPLQIVTEIKERGLSLPGVNIIEDAIRTVTQGDVIPHEIGTVGPIYAEEYEELAEQGYDLDDTVGKSGIEQAMESELRGKDGTKTISVQNGTVVSSEVTVPVQAGHTVMLTVDSTFQKDLQNILGNFIKNFDKLRDAKTEGEGLGKVTSGALVVLDTTDNSVLGLATYPTYNLNDYTKDYDKLASDETSPLLDRATVGLYRPGSTFKTITATAGLNEGIVAGSSTYFCGHSYEYKGHTYYCTGSHGNISVVQALQYSCNIYFYQLSEALTIDNLTKYAELYGLGSPTGIETGDAAGRFANPETYAEYGWEWTVGHVLQAAIGQSETAVTPLQMAVVASTIANKGVRYQPHLVDSLWDYNLTEKIKDIEPTVAETIPIQHDDVYTYIQQGMIAASVTNMPDKYSLADLGYDVAIKTGTPQAGGGRVQDSFFIGYAPADNPKIAFACVVEGAEYSKYMIRDVLKAYERMETRQKTNMEKNTTSNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5V043</accession>
    <name>R5V043_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ppx/GppA phosphatase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01373</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000145">
        <property type="protein sequence ID" value="CCZ83610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01373"/>
    <dbReference type="EnsemblBacteria" id="CCZ83610">
        <property type="protein sequence ID" value="CCZ83610"/>
        <property type="gene ID" value="BN566_01373"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003695">
        <property type="entry name" value="Ppx_GppA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02541">
        <property type="entry name" value="Ppx-GppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ppx-GppA" evidence="1">
        <location>
            <begin position="17"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="298" mass="33097" checksum="86DCF357F908D6C0" modified="2013-07-24" version="1">MNVALIDMGSNSIRLAVYKVEQKDFEPIFSKRYMTGLASYVKEKQLTAAGVEQACIVLLDAKSLLEQLSVDQTTVFATASLRNIKNTEAVVAAIQQKTGYTVQILSGHEEAFLDYFGVMHGAPMEEGALVDIGGGSTEITTFAADGPAFMESMPVGALRLHTEMVEKFLPKEAEAAQIQERVLREWKKIKADKLPHYDLLCAVGGTARAVLQLIQTQQAPLPDANSFTAEQFQKLKNQLLKRDETAKKLILQVCPERVHTILPGILILDVIIRQLHTHLIYVSPYGVREGYLLRHYLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5V083</accession>
    <name>R5V083_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01401</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000148">
        <property type="protein sequence ID" value="CCZ83665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83665">
        <property type="protein sequence ID" value="CCZ83665"/>
        <property type="gene ID" value="BN566_01401"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="84" mass="9566" checksum="3167FEC465D6D92C" modified="2013-07-24" version="1">MCTMCLKKDAKRHMEKRRENGALPRYMFGDGYHCVTCGKDIDNGKKQCDECQYNAAHALEIARGKIKGGFRNHKLVFGKTGRKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0D1</accession>
    <name>R5V0D1_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TsaA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01749</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the tRNA methyltransferase O family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000180">
        <property type="protein sequence ID" value="CCZ84023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01749"/>
    <dbReference type="EnsemblBacteria" id="CCZ84023">
        <property type="protein sequence ID" value="CCZ84023"/>
        <property type="gene ID" value="BN566_01749"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09281">
        <property type="entry name" value="UPF0066"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023370">
        <property type="entry name" value="TrmO-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041369">
        <property type="entry name" value="TrmO_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023368">
        <property type="entry name" value="UPF0066_cons_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040372">
        <property type="entry name" value="YaeB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036413">
        <property type="entry name" value="YaeB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036414">
        <property type="entry name" value="YaeB_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12818">
        <property type="entry name" value="PTHR12818"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01980">
        <property type="entry name" value="TrmO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18389">
        <property type="entry name" value="TrmO_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118196">
        <property type="entry name" value="SSF118196"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00104">
        <property type="entry name" value="tRNA_TsaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01318">
        <property type="entry name" value="TSAA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51668">
        <property type="entry name" value="TSAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="TsaA-like" evidence="3">
        <location>
            <begin position="1"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00033753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="227" mass="25661" checksum="3FFF7E9CC333DE03" modified="2013-07-24" version="1">MHPIAHIENAFQTKFGIPRQSGLVSSFPSKIIFEPEYRNPDAFRGLDAYSHLWLIWEFSDVIRKTWSPTVRPPRLGGNQRMGVFATRSPFRPNPIGLSVVTLDQISWDEPNAPILYVNGADLMNGTPIYDIKPYLPHIDSHPEATGGFAVQVADYALEVVFPEKWLIKIPKSLQTAICEILSQDPRPSYQHDPERVYGFYFADLEIRFTVQDSVLTVCSVSEIAKGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0K8</accession>
    <name>R5V0K8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">L-lactate dehydrogenase</fullName>
            <shortName evidence="6">L-LDH</shortName>
            <ecNumber evidence="4 6">1.1.1.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ldh</name>
        <name evidence="12" type="ORF">BN566_01535</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the conversion of lactate to pyruvate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>(S)-lactate + NAD(+) = H(+) + NADH + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:23444"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16651"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.27"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="6">Allosterically activated by fructose 1,6-bisphosphate (FBP).</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 6">Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the LDH/MDH superfamily. LDH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.27" evidence="4 6"/>
    <dbReference type="EMBL" id="CBAR010000164">
        <property type="protein sequence ID" value="CCZ83800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01535"/>
    <dbReference type="EnsemblBacteria" id="CCZ83800">
        <property type="protein sequence ID" value="CCZ83800"/>
        <property type="gene ID" value="BN566_01535"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00554">
        <property type="reaction ID" value="UER00611"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004459">
        <property type="term" value="F:L-lactate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00488">
        <property type="entry name" value="Lactate_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011304">
        <property type="entry name" value="L-lactate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000102">
        <property type="entry name" value="Lac_mal_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00086">
        <property type="entry name" value="LLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="SSF56327"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01771">
        <property type="entry name" value="L-LDH-NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0520">NAD</keyword>
    <keyword evidence="6 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ldh_1_N" evidence="10">
        <location>
            <begin position="9"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Ldh_1_C" evidence="11">
        <location>
            <begin position="149"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="124"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="152"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6 7">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 8">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="122"/>
            <end position="124"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>allosteric activator</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>allosteric activator</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CCZ83800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="316" mass="33809" checksum="39FA716E908CAF3B" modified="2013-07-24" version="1">MDFVKKGKKVTVIGAGNVGASIAFTLAIKGLCSELLLVDINKPKAKGEAMDIMQGTAFCPAVDVRDGDYADAVNSDLVIITVGIARKPGQTRIDLCKTNAKIMASVMPEITKYAPNACYIIVSNPVDVLTYEAIQVSGLNPKHVFGSGTVLDSSRLRTCLADHANLNIKNIHAYVFGEHGDSSMIPWSLSSIDGMKFDDYCAHIDNLGDKDAIEQEVRAGGAEVIKCKGATYYAIALSVSQIAEAILRDTKMILTVSTLQDGSRYGVKDVCLSLPCVVGANGIEREITPPLLPEEEEAVRKSAAALREVIDAMKAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0T2</accession>
    <name>R5V0T2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01609</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CdaR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000169">
        <property type="protein sequence ID" value="CCZ83880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01609"/>
    <dbReference type="EnsemblBacteria" id="CCZ83880">
        <property type="protein sequence ID" value="CCZ83880"/>
        <property type="gene ID" value="BN566_01609"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2840">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041522">
        <property type="entry name" value="CdaR_GGDEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025736">
        <property type="entry name" value="PucR_C-HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042070">
        <property type="entry name" value="PucR_C-HTH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17853">
        <property type="entry name" value="GGDEF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13556">
        <property type="entry name" value="HTH_30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GGDEF_2" evidence="3">
        <location>
            <begin position="126"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="HTH_30" evidence="2">
        <location>
            <begin position="295"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="361" mass="40816" checksum="D9B97E2A6E93B504" modified="2013-07-24" version="1">MSNRLFQGLVHQMRDTIDATVGVVDDSATIIACSDLTKVGTTNEFVSLDLSDVHDIFIRDGYTYKPFGSPNKPEYAVFVEGVDDAAAKYANILAIALFNVKQYYDEKYDRNNFIKNVVLDNVLPGDIVIKARELHFNAEVSRVVLLIRIVSTNDVSAYDVIQNLFPDKNKDFVFTISESDIVLVKEIKGAVDAKDLEKLARSIADTLSGEFYTRVNVGIGTIVDSIKELSRSFKEAQTALEVGKVFDTDKAIVSYDNLGIARLIYHLPTTLCETFLHEVFKRGSIESLDHETLFTIQKFFENNLNVSETSRKLFVHRNTLVYRLEKIKKLTGLDLREFDHAIIFKIALMVKRYLSANPAKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0Y2</accession>
    <name>R5V0Y2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative ribonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00192</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000204">
        <property type="protein sequence ID" value="CCZ84263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00192"/>
    <dbReference type="EnsemblBacteria" id="CCZ84263">
        <property type="protein sequence ID" value="CCZ84263"/>
        <property type="gene ID" value="BN566_00192"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022712">
        <property type="entry name" value="Beta_Casp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011108">
        <property type="entry name" value="RMMBL"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10996">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07521">
        <property type="entry name" value="RMMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01027">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="13"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-Casp" evidence="2">
        <location>
            <begin position="250"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="529" mass="59192" checksum="3E882C2688DFB795" modified="2013-07-24" version="1">MKLLFIGATHEVTGSCTYLEACGKRILIDYGMEQGRDVYENIRVPCAPSEIDYVFLTHAHIDHSGLLPLLYAGGFRGEIHATQATVSLCQIMLRDSAHIQEFEAEWRNRKAKRSGGDIYTPLYTMQEALGSLEHFVPHPYGEKISIADGITIRFLDAGHLLGSSSIEIWLEENGISKKLLFSGDIGNFHQPLIRDPQYADTADYVIMESTYGNRLHEKPKNYIQDFVQILLETFQRGGSVIIPSFAVGRTQELLYFLKQIKDQNLLGKYQDVPIYVDSPLAIEATEIFQRNKESCFDEEALAYVQRGENPIGVSGLRTAVTSEASMAINADTRSKIVISASGMCEAGRIKHHLKHNLWKPENTILFVGYQAVGTLGRRLLEGANYVTLFGESVKVAAQIRQLQGMSGHADQKGLELWIQHVQQVKKVFVIHGETTAADTFTELLNEQGISAYAPYSGTEFDVASGQFLHEAEPVYLEKPKSVKASATYDRLQIALDRVTAYIQNARGYSNKELAKMADQLTQLCNKWEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1E4</accession>
    <name>R5V1E4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">4-alpha-glucanotransferase</fullName>
            <ecNumber evidence="3 7">2.4.1.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5 7">Amylomaltase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6 7">Disproportionating enzyme</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_00305</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>Transfers a segment of a (1-&gt;4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1-&gt;4)-alpha-D-glucan.</text>
            <dbReference type="EC" id="2.4.1.25"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the disproportionating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.25" evidence="3 7"/>
    <dbReference type="EMBL" id="CBAR010000224">
        <property type="protein sequence ID" value="CCZ84398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00305"/>
    <dbReference type="EnsemblBacteria" id="CCZ84398">
        <property type="protein sequence ID" value="CCZ84398"/>
        <property type="gene ID" value="BN566_00305"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004134">
        <property type="term" value="F:4-alpha-glucanotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102500">
        <property type="term" value="F:beta-maltose 4-alpha-glucanotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003385">
        <property type="entry name" value="Glyco_hydro_77"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32438">
        <property type="entry name" value="PTHR32438"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02446">
        <property type="entry name" value="Glyco_hydro_77"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00217">
        <property type="entry name" value="malQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ84398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="509" mass="58549" checksum="D55922166801BD20" modified="2013-07-24" version="1">MRRSGILMHISSLPSRYGIGKLGASAYQFADFLKKSGVSVWQILPLSPTSYGDSPYQSFSAYAGNPYFIDFEQLEEQGLLEHTDYADANWGSPNKVDYERLYQMCFPILKKAFQAFDRTNPDYVAFCEKHRAWLPDYALFMALKFAHNGKAWTEWEPELAMRNAESLQKARSKYIEDVQFFQVIQYWFYSQWDKLKTYCNENGISLIGDMPIYVSYDSVEVWSQPELFELDRQHRPIAVAGCPPDVFSPTGQLWGNPLYDWDYHKKTGYAWWIQRLAFATSIYDTVRIDHFRGFESYYSIPYGNPTAEIGEWRKGPNTALFEAAQAALGDLSIIAEDLGFITPEVQKMLDACGYPGMKVTQFAFSDGAKNTYLPQNYKTTNCVAYTGTHDNMTLNGWVYGASDKEISFAKRYLRVRDRKDLPESMLRITWGSIADLAIGQVQDFLQEPANARMNTPSTAFGNWQYRTTAKQFNHKLSKKIYQLNALYNRLPEEEDSAAETDTKEIELED</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1F1</accession>
    <name>R5V1F1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">LPXTG-motif cell wall anchor domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01801</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000186">
        <property type="protein sequence ID" value="CCZ84075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01801"/>
    <dbReference type="EnsemblBacteria" id="CCZ84075">
        <property type="protein sequence ID" value="CCZ84075"/>
        <property type="gene ID" value="BN566_01801"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002105">
        <property type="entry name" value="Dockerin_1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00404">
        <property type="entry name" value="Dockerin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004394920" evidence="1">
        <location>
            <begin position="29"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="2">
        <location>
            <begin position="555"/>
            <end position="617"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="617" mass="67538" checksum="8DBC5089834F8AFA" modified="2013-07-24" version="1">MKRKRIKAAVCAAVMAFTLFGSLCPVDYHFMRETSVTASAEAAIWDGTEDTSWYYREHDVIVSENGERIAIFNIRTAEELAGLAKLVRNGNSMKNTMINLVSDVTLNDTTNFDNWDEQPPANNWTAIGAVPIEAPNVNVVMTTQPECRNTVFAGMFNGNGHTISGMYSLHHNYAGLFARTSGIITNVIVKDSYIQCINTQKVESGDTALWEACAGGITAICDRGVISHCEFDGKIFASGLNQVGYGTHGSFAGGIVGKFSDDENGVAAVVMMFALVPAGFLINPALFLTASGQQVITDPGIYSCINRGSIYTEHGTDENGAGGIIGAGGLYTVRNPDFAVFYCLNLGEVHADHQNIGGMVGEGYKFSEQKSYYTNCERSSENDDAINFVDAGMNLEEVAEEMDGNYKYENGAIMLNFDGNTKEEPSETMKKETFTRETVKKNITMPAPELSCSFLTHYFGTVVDKDNVQVSTSTNSEVSNWSIDVAGDPKFTDIYLNDFTIYDLVPEVTYYVRVQGRTLSDRTNPECEYTDYGYLSITLKDDGSLDVNKDFVAEDEKIKGDVNADGALNVADVVLFQKWLLAVPGVKLDEGEMADLYEDGNLNVLDLAVMKQKLSIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1F5</accession>
    <name>R5V1F5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01806</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000186">
        <property type="protein sequence ID" value="CCZ84080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84080">
        <property type="protein sequence ID" value="CCZ84080"/>
        <property type="gene ID" value="BN566_01806"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="172" mass="19951" checksum="ECA7686BCA9BE03F" modified="2013-07-24" version="1">MITLKELIHATADTIKIDWLKVEETIGFPLHYDLKNFYSRILCAEQKTISGDFILNETYFIQKTGNERFDTWFSFNGCEGRLSYELYPIRMLENAHIQIKYAFTSWTGGHDFGHRAWIGVINTDMGDFLILFNNDTGNVEWIDCGYGYFDVYEENPNGIIANDIDGFLSKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1H3</accession>
    <name>R5V1H3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">ATP synthase subunit c</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">ATP synthase F(0) sector subunit c</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">F-type ATPase subunit c</fullName>
            <shortName evidence="13">F-ATPase subunit c</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Lipid-binding protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">atpE</name>
        <name evidence="15" type="ORF">BN566_00325</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 13">F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation.</text>
    </comment>
    <comment type="function">
        <text evidence="13">Key component of the F(0) channel; it plays a direct role in translocation across the membrane. A homomeric c-ring of between 10-14 subunits forms the central stalk rotor element with the F(1) delta and epsilon subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cell membrane</location>
            <topology evidence="13">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the ATPase C chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000225">
        <property type="protein sequence ID" value="CCZ84418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00325"/>
    <dbReference type="EnsemblBacteria" id="CCZ84418">
        <property type="protein sequence ID" value="CCZ84418"/>
        <property type="gene ID" value="BN566_00325"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045263">
        <property type="term" value="C:proton-transporting ATP synthase complex, coupling factor F(o)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01396">
        <property type="entry name" value="ATP_synth_c_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005953">
        <property type="entry name" value="ATP_synth_csu_bac/chlpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000454">
        <property type="entry name" value="ATP_synth_F0_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020537">
        <property type="entry name" value="ATP_synth_F0_csu_DDCD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038662">
        <property type="entry name" value="ATP_synth_F0_csu_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035921">
        <property type="entry name" value="F/V-ATP_Csub_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10031">
        <property type="entry name" value="PTHR10031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00124">
        <property type="entry name" value="ATPASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81333">
        <property type="entry name" value="SSF81333"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01260">
        <property type="entry name" value="ATP_synt_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00605">
        <property type="entry name" value="ATPASE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 13" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 13" id="KW-0138">CF(0)</keyword>
    <keyword evidence="6 13" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="8 13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 13" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="49"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-synt_C" evidence="14">
        <location>
            <begin position="2"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="site" description="Reversibly protonated during proton transport" evidence="13">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CCZ84418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="68" mass="6636" checksum="4337141C1815F3A6" modified="2013-07-24" version="1">MIAAGIAVLTGLGAGLGIGIATSKAAEAIARQPEAAGDINKTLLLGSALAEATAIYGFVVALLLVIMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1J0</accession>
    <name>R5V1J0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01855</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000191">
        <property type="protein sequence ID" value="CCZ84130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01855"/>
    <dbReference type="EnsemblBacteria" id="CCZ84130">
        <property type="protein sequence ID" value="CCZ84130"/>
        <property type="gene ID" value="BN566_01855"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_25" evidence="1">
        <location>
            <begin position="44"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="251" mass="29209" checksum="844D68D202DF3B08" modified="2013-07-24" version="1">METGYEAFSFYYDQLTENVEYPQRAAYFHKLIQKYCQSEGVVLLDLACGTGSLSEEFAKLGYDVLGVDYSYGMLNQAMQKKLEHNLPIQYICQDMRELDLYGTVDITVCALDSLNHLDSFSDVQTVFQQVFDVTESGGVFLFDMNTPYKHREVLGNQIYLYDTDDVYCVWENQYEEPDNQVTIQLHFFARQEDDTYVRSEECMTERAYPPEQVTAALQQIGWEVLGCYHADTEEPLREDSQRMIFAVRKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1L3</accession>
    <name>R5V1L3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01885</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0751 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000193">
        <property type="protein sequence ID" value="CCZ84160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01885"/>
    <dbReference type="EnsemblBacteria" id="CCZ84160">
        <property type="protein sequence ID" value="CCZ84160"/>
        <property type="gene ID" value="BN566_01885"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016772">
        <property type="entry name" value="UCP020408"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10087">
        <property type="entry name" value="DUF2325"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="103" mass="11176" checksum="4A3E231D6A571B2E" modified="2013-07-24" version="1">MSVVIIGGNECMSRKYTDICQEHGCKAKVFPKETGPLEKKIGSPDLMILFTNTVSHKMIQGAMKAAKKNSVPVARIHSSSATALRNLLCEHCPICSNCQAKAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1T7</accession>
    <name>R5V1T7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Putative mRNA interferase YoeB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_00189</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the YoeB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000203">
        <property type="protein sequence ID" value="CCZ84260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00189"/>
    <dbReference type="EnsemblBacteria" id="CCZ84260">
        <property type="protein sequence ID" value="CCZ84260"/>
        <property type="gene ID" value="BN566_00189"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2310.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035093">
        <property type="entry name" value="RelE/ParE_toxin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009614">
        <property type="entry name" value="YoeB_toxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38039">
        <property type="entry name" value="PTHR38039"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06769">
        <property type="entry name" value="YoeB_toxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143011">
        <property type="entry name" value="SSF143011"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02116">
        <property type="entry name" value="toxin_Txe_YoeB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00030388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ84260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="89" mass="10745" checksum="10FB88E6466CF383" modified="2013-07-24" version="1">MSEKIWSDDAWDDYLYWQTQDKKTLKRINQLIKDIERNGCLEGIGQPEALKNNLHGEYSRRINEKDRLVYHVENDRIYIVSCRGHYDDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5V233</accession>
    <name>R5V233_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Multidrug export protein MepA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_00454</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family. MepA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000251">
        <property type="protein sequence ID" value="CCZ84593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84593">
        <property type="protein sequence ID" value="CCZ84593"/>
        <property type="gene ID" value="BN566_00454"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13143">
        <property type="entry name" value="MATE_MepA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045070">
        <property type="entry name" value="MATE_MepA-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006603">
        <property type="entry name" value="DinF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0046">Antibiotic resistance</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="18"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="93"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="197"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="240"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="281"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="322"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="367"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="398"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="425"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ84593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="460" mass="49664" checksum="5770E0BCBF7CF0F2" modified="2013-07-24" version="1">MSTTVASNPLGSESVTKLMLKFSIPSIISMLVGALYNIVDQLFIGQAVGMLGNAATNVAFPLSTSCIALALLFGIGGAACFNLKMGAGEKEDAAYAFGNAFSMLVISGVLLAAVSLIFLNPLLRLFGAPDNVMPYAEEYVRITAFGFPLLIITAGGGHLIRADGSPTMTMVCNLAGAIINTILDAIFVLGLDWGMAGAAYATVIGQVVSALIVFVYLLRYRKTDLQLKHFIPHFSHARKIAAIGMASFLNQVAIMIVQIILNNMLEHYGSLSVYGDAIPLASAGIIMKVYQIIFSVVIGLGQGIQPIVSFNYGAKKYDRVWSAYRIAVITGGILCVVAFFLFELFPKPILSLFGDGSDQYYQFGVRFFRIFLFFTFIDFLQPISTTFFTAIGKAVKGAFLSLTRQILFFLPPLLILPLFMGIDGILFAGPIADFLSAAVTVIMIFIEWKLMKKEAPALAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5V242</accession>
    <name>R5V242_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SHSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00456</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000253">
        <property type="protein sequence ID" value="CCZ84603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00456"/>
    <dbReference type="EnsemblBacteria" id="CCZ84603">
        <property type="protein sequence ID" value="CCZ84603"/>
        <property type="gene ID" value="BN566_00456"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045045">
        <property type="entry name" value="RTM2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43670">
        <property type="entry name" value="PTHR43670"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="SSF49764"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SHSP" evidence="3">
        <location>
            <begin position="27"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="139" mass="16306" checksum="1AA10DFC95C436A2" modified="2013-07-24" version="1">MYGLTAFERDPFSLLDPFSDFDDRFVSKETVRSFKTDIREEDDKFIMEAELPGFEKGEIQLDVNGDTLTLSAVHQQEQVDKSKEHYICRERSYGSYQRRFDISGIDADQIEAEYKNGILYMILPKKQPKVPANRRLEIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>R5V2F0</accession>
    <name>R5V2F0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Phosphomannomutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN566_00379</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000239">
        <property type="protein sequence ID" value="CCZ84480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00379"/>
    <dbReference type="EnsemblBacteria" id="CCZ84480">
        <property type="protein sequence ID" value="CCZ84480"/>
        <property type="gene ID" value="BN566_00379"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016868">
        <property type="term" value="F:intramolecular transferase activity, phosphotransferases"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005843">
        <property type="entry name" value="A-D-PHexomutase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005841">
        <property type="entry name" value="Alpha-D-phosphohexomutase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00408">
        <property type="entry name" value="PGM_PMM_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00509">
        <property type="entry name" value="PGMPMM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="SSF53738"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="SSF55957"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4 6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PGM_PMM_I" evidence="8">
        <location>
            <begin position="43"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="PGM_PMM_II" evidence="9">
        <location>
            <begin position="208"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="PGM_PMM_III" evidence="10">
        <location>
            <begin position="322"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="PGM_PMM_IV" evidence="7">
        <location>
            <begin position="500"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ84480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="573" mass="63169" checksum="C7F7CED5C0BF8FDF" modified="2013-07-24" version="1">MTEMELYQSWRKNAIDDPDLQSELSAIENDAEAIQDRFYRDLAFGTGGLRGVIGAGTNRMNIYTVRKATQGLANYVKEAFSEPSVAISYDSRIKSTDFAKAAAEVLAANGVKVHIYTELKPTPMLSFAVRALHCSAGIMVTASHNPAKYNGYKAYGSDGCQMTIDAADAVLAKINALDIFNDVKHMPFDEALAAGMISYIGDDVIEDYFRNVLAQGINTDLCAKSGLKIVYTPLNGTGNKPVRTILNRIGIQEVTVVKEQENPDGNFTTCPYPNPEIREALQLGLQYCDQVKPDLLLATDPDADRVGIAVPDGKGGYALFSGNEVGAMLLEYICEQRIQKGTMPKHPVAVKTIVTTDIVEAICKAYDVELIEVLTGFKFIGEQIGFLEEKGEEDRYIFGFEESYGYLAGSYVRDKDAVVASMLICEMAAYYRTKGISMMQARENLYQKYGVYVHAQHSFTFEGESGMIRMQNIMEHLRTHRPEQIDNLKVIQFADYEKQVSIDLTTGTETTITLPKSNVLSFTLEQGAKVIIRPSGTEPKIKAYYTTTAATEAEAAAKKDELDAAFSAIMLAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5V2Q7</accession>
    <name>R5V2Q7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Diadenosine tetraphosphate (Ap4A) hydrolase and other HIT family hydrolases</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00416</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000244">
        <property type="protein sequence ID" value="CCZ84555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00416"/>
    <dbReference type="EnsemblBacteria" id="CCZ84555">
        <property type="protein sequence ID" value="CCZ84555"/>
        <property type="gene ID" value="BN566_00416"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019808">
        <property type="entry name" value="Histidine_triad_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001310">
        <property type="entry name" value="Histidine_triad_HIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23089">
        <property type="entry name" value="PTHR23089"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
        <property type="entry name" value="HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00332">
        <property type="entry name" value="HISTRIAD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="SSF54197"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00892">
        <property type="entry name" value="HIT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIT" evidence="2">
        <location>
            <begin position="5"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Histidine triad motif" evidence="1">
        <location>
            <begin position="97"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="119" mass="12850" checksum="8C061558B68FA451" modified="2013-07-24" version="1">MEDCLFCSIAAGKIPSTKVYEDDTVYVFKDIAPIAPIHYLLIPKQHIACAADITPENSGVVAHIYEVAAQIAAKEGISDGFRIITNCGENAGQTVHHLHFHMLAGVKMGWGKESVQAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5V2R2</accession>
    <name>R5V2R2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Cytidylate kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00421</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000246">
        <property type="protein sequence ID" value="CCZ84560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00421"/>
    <dbReference type="EnsemblBacteria" id="CCZ84560">
        <property type="protein sequence ID" value="CCZ84560"/>
        <property type="gene ID" value="BN566_00421"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="202" mass="22669" checksum="A1D95D2490B2D458" modified="2013-07-24" version="1">MTPYAITITRQFGSEGREIGKQLAERLHCHYYDRSVIEQEAAQKDESLAALVALKQVEGYYKMAYPLGIGSSLKQEKMFQAQSEVLLEHAAKENCVIVGRCADYVLRNQERVLRCYLYAPDAYRASSVASLNLSTASEYQPLIDEVDKARASYYLKYTGYPVDNLELRDLVVNTACLGLEGTVEMLAAFAVKKFGLNLEEQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5V2T2</accession>
    <name>R5V2T2_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ferredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_00441</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000247">
        <property type="protein sequence ID" value="CCZ84580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00441"/>
    <dbReference type="EnsemblBacteria" id="CCZ84580">
        <property type="protein sequence ID" value="CCZ84580"/>
        <property type="gene ID" value="BN566_00441"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000813">
        <property type="entry name" value="7Fe_ferredoxin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00354">
        <property type="entry name" value="7FE8SFRDOXIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="3 5" id="KW-0408">Iron</keyword>
    <keyword evidence="4 5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="29"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU365098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ84580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="56" mass="5713" checksum="34B67C9AEF83933A" modified="2013-07-24" version="1">MAYKITDACLSCGSCADACPVEAIKEGDSTYVIDADSCISCGSCADTCPVQAIVED</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5V3S9</accession>
    <name>R5V3S9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00004</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000001">
        <property type="protein sequence ID" value="CCZ82749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82749">
        <property type="protein sequence ID" value="CCZ82749"/>
        <property type="gene ID" value="BN566_00004"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="58" mass="6257" checksum="4F3B7EFA7811A863" modified="2013-07-24" version="1">MVCIIGILIGVLVLGTGIYYLHQEKHDSESRKIYTIVSVVGALLAVGAAVWLILGYLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5V418</accession>
    <name>R5V418_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glycine radical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00664</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000012">
        <property type="protein sequence ID" value="CCZ82859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00664"/>
    <dbReference type="EnsemblBacteria" id="CCZ82859">
        <property type="protein sequence ID" value="CCZ82859"/>
        <property type="gene ID" value="BN566_00664"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019777">
        <property type="entry name" value="Form_AcTrfase_GR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001150">
        <property type="entry name" value="Gly_radical"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01228">
        <property type="entry name" value="Gly_radical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00850">
        <property type="entry name" value="GLY_RADICAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51149">
        <property type="entry name" value="GLY_RADICAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0556">Organic radical</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycine radical" evidence="3">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="modified residue" description="Glycine radical" evidence="2">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ82859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="79" mass="9001" checksum="F8FC1CE2ABA48F6B" modified="2013-07-24" version="1">MANEKQIDNLVELLDGYAEKGGHHLNVNVLNRETLLDAQQHPEKYPQLTIRVSGYAVNFIKLTKEQQDDVISRTFHAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5V4D5</accession>
    <name>R5V4D5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00769</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000033">
        <property type="protein sequence ID" value="CCZ82969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82969">
        <property type="protein sequence ID" value="CCZ82969"/>
        <property type="gene ID" value="BN566_00769"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="180" mass="20181" checksum="176DAEEBBA88B784" modified="2013-07-24" version="1">MHCWNCGHIVNGDEKKCPSCGASRKIEKGTKIHYCPNCGKGIWGKEKCKYCSFDVILKKVIQEVEAVDGKKTASTVRACASVKASDDLNVDKQGPPQNVKNKTSDESKKEAILKKLQQQDSAVQNQVLKRLEDAKTQEISEKTADFFLSLPALEKMNPKIQQEARFVLSTRYPDFVKNEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5V4L0</accession>
    <name>R5V4L0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00807</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000038">
        <property type="protein sequence ID" value="CCZ83014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00807"/>
    <dbReference type="EnsemblBacteria" id="CCZ83014">
        <property type="protein sequence ID" value="CCZ83014"/>
        <property type="gene ID" value="BN566_00807"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd16332">
        <property type="entry name" value="Prp-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007422">
        <property type="entry name" value="Peptidase_Prp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036764">
        <property type="entry name" value="Peptidase_Prp_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39178">
        <property type="entry name" value="PTHR39178"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04327">
        <property type="entry name" value="Peptidase_Prp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118010">
        <property type="entry name" value="SSF118010"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="106" mass="11363" checksum="6F80F7287212AC2F" modified="2013-07-24" version="1">MITATFYHRNQRLTGVSVSGHAGYADAGQDVICASVSASVQLTANLLTEIYQLPAEIAVEENCISISVDAIQDVNAERLLQGLLLQMQLLTEEAPDCIQIRNASAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>R5V4P8</accession>
    <name>R5V4P8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Glutamine-dependent NAD(+) synthetase</fullName>
            <ecNumber evidence="5 6">6.3.5.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5 6">NAD(+) synthase [glutamine-hydrolyzing]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">nadE</name>
        <name evidence="9" type="ORF">BN566_00857</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses L-glutamine as a nitrogen source.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>ATP + deamido-NAD(+) + H2O + L-glutamine = AMP + diphosphate + H(+) + L-glutamate + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:24384"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 5 6">Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the NAD synthetase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5 6">In the C-terminal section; belongs to the NAD synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.1" evidence="5 6"/>
    <dbReference type="EMBL" id="CBAR010000051">
        <property type="protein sequence ID" value="CCZ83069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00857"/>
    <dbReference type="EnsemblBacteria" id="CCZ83069">
        <property type="protein sequence ID" value="CCZ83069"/>
        <property type="gene ID" value="BN566_00857"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00334"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003952">
        <property type="term" value="F:NAD+ synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008795">
        <property type="term" value="F:NAD+ synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00553">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02090">
        <property type="entry name" value="NadE_glutamine_dep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014445">
        <property type="entry name" value="Gln-dep_NAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041856">
        <property type="entry name" value="NAD+_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003694">
        <property type="entry name" value="NAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23090">
        <property type="entry name" value="PTHR23090"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006630">
        <property type="entry name" value="NADS_GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="SSF56317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00552">
        <property type="entry name" value="nadE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 6 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 6 7" id="KW-0520">NAD</keyword>
    <keyword evidence="3 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="8">
        <location>
            <begin position="6"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for glutaminase activity" evidence="5">
        <location>
            <position position="46"/>
        </location>
    </feature>
    <feature type="active site" description="For glutaminase activity" evidence="5">
        <location>
            <position position="115"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; for glutaminase activity" evidence="5">
        <location>
            <position position="173"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="352"/>
            <end position="359"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="438"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="462"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="467"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="472"/>
            <end position="475"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="599"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR006630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ83069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="638" mass="70014" checksum="486578BC58773020" modified="2013-07-24" version="1">MKNGFFPIACATPPLRVADCTYNAEQIISLTREAAKNGSKLVVFPELCITGYTCGDLFLQQILLDGALQALRTILKETSNLSCICVVGLPIRHTGAIYNCAAVCYQGSVLGIVPKQHLPNYGEFYEQRHFTPGIPADGKTFWQADGIAPCPFGAQQLFQCAEAPDFTFGVELCEDVWVADTPSVAMARMGATLIVNLSCSDEIIGKAAYRRTILQAKSGSLLCAYAYADAGMGESTQDMVFAGHNLILENSAILAESKLFSDGLLYAEPDLQRMAAERQRSNSFQPLPLTTVCTRFFMPLTETALHRFFSPTPFVPAGAAELSERCETILTLQATGLATRLRHIGCKTAVVGLSGGLDSTLALIVMVHAFDLLGLPRSGMLAVTMPCFGTTDRTYQNACRLAKAYGATLREIPIQKSVRQHFADIGQDESVHDVTYENSQARERTQVLMDLANQCGGIVVGTGDLSELALGWATYNGDHMSMYAVNASIPKTLVRYLVQYEASKTPEALRAVLLDVLDTPVSPELLPPEPDGTIAQKTEDLVGPYELHDFFLYYVLRFGFTPSKIYHLAQKALGGKYETAVILKWLRSFYRRFFSQQFKRSCLPDAPKVGSVTLSPRGDWRMPSDACRTLWLADLEQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5V565</accession>
    <name>R5V565_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_01050</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01050"/>
    <dbReference type="EnsemblBacteria" id="CCZ83269">
        <property type="protein sequence ID" value="CCZ83269"/>
        <property type="gene ID" value="BN566_01050"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025302">
        <property type="entry name" value="DUF4162"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13732">
        <property type="entry name" value="DUF4162"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="6"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ83269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="316" mass="35827" checksum="4604B5FA0AA24D0A" modified="2013-07-24" version="1">MEQVVVKIDNLVKRYKELIALDHFSLEIREGEIFGLLGPNGSGKTTTINCLLSLLSFDKGKIEIFGKEMSPVQYDIKRQIGVIMQNVAVFQELTVYENIDYFCGLYIRDKATRKQYVMDAVHFTGLEKFLKFYPKKLSGGLLRRLNIACGIAHKPKLIILDEPTVAVDPQSRNQILEGIQKLNANGATIIYTSHYMEEVEQICTRIAIMDQGKKIAEGTKEELKQIIKNTETITIEVLMLPESVLQSIKALEHIYQATYEQEELKVYCSGGMHNLVDVLHILQEQQVAIGRVYTTLPTLNDVFLEITGKALRDQEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5V586</accession>
    <name>R5V586_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Sulfate/thiosulfate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_01070</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the prokaryotic sulfate-binding protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01070"/>
    <dbReference type="EnsemblBacteria" id="CCZ83289">
        <property type="protein sequence ID" value="CCZ83289"/>
        <property type="gene ID" value="BN566_01070"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901681">
        <property type="term" value="F:sulfur compound binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902358">
        <property type="term" value="P:sulfate transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01005">
        <property type="entry name" value="PBP2_CysP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000957">
        <property type="entry name" value="Sulphate/thiosulphate-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034408">
        <property type="entry name" value="Sulphate/thiosulphate_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005669">
        <property type="entry name" value="Thiosulph/SO4-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30368">
        <property type="entry name" value="PTHR30368"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00971">
        <property type="entry name" value="3a0106s03"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00401">
        <property type="entry name" value="PROK_SULFATE_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00757">
        <property type="entry name" value="PROK_SULFATE_BIND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0574">Periplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0764">Sulfate transport</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004406763" evidence="7">
        <location>
            <begin position="30"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="341" mass="37438" checksum="DFBB16F48BEB02A1" modified="2013-07-24" version="1">MKHTTITKVIGISLSAALLASVTTGCGSASNGDTVQITNVSYDPTRELYTAYNELFAKHWKEKTGQDVEVTQSHGGSGKQALEVANGLEADVVTLALEYDVDAIQDAGLIDDGWISEFDDDSAPYTSTIVFLVRKGNPKNIQDWDDLVKDGVGVITPNPKTSGGARWNYLAAWAYATQEYGDDEAKTEDFMKKLYQNVLVLDSGARGATTTFVENGQGDVLLAWENEAYLSIKDAPDDYEIVTPSISILAQPSVAVVDDVVDRKGTREVATEYLSYLYSDEAQRIAGDNYYRPSNPDILKEYADVFDLNVNLVTIQDFGGWEEAQKTHFADGGIFDKIYEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>R5V5A6</accession>
    <name>R5V5A6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">FeS assembly ATPase SufC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_01095</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. Ycf16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01095"/>
    <dbReference type="EnsemblBacteria" id="CCZ83314">
        <property type="protein sequence ID" value="CCZ83314"/>
        <property type="gene ID" value="BN566_01095"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03217">
        <property type="entry name" value="ABC_FeS_Assembly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010230">
        <property type="entry name" value="FeS-cluster_ATPase_SufC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43204">
        <property type="entry name" value="PTHR43204"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01978">
        <property type="entry name" value="sufC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="7"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="39"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ83314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="249" mass="27545" checksum="FB60F4F71267A60D" modified="2013-07-24" version="1">MSQETLLQIQNLKVNVGETEILHGIDLDIQENEVHVLMGPNGTGKSTLGSTLMGNPEYTITGGDIFYKGEKINDLPDNERAKKGIFLSFQNPIEIPGITVSAFIRSAVEAKTGKRVRFFSFKKELEEKMELLQMDPSYAERDLNVGFSGGEKKKTEILQMLMLHPELVILDETDSGLDVDAVKTVSQGIADYRKSSKGSLLIITHSTKILESLHVDKTHIMVNGKIVKTGDSSLIDEINENGFSAYETL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5V5L2</accession>
    <name>R5V5L2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Heavy metal-associated domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00127</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000101">
        <property type="protein sequence ID" value="CCZ83419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00127"/>
    <dbReference type="EnsemblBacteria" id="CCZ83419">
        <property type="protein sequence ID" value="CCZ83419"/>
        <property type="gene ID" value="BN566_00127"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017969">
        <property type="entry name" value="Heavy-metal-associated_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="SSF55008"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01047">
        <property type="entry name" value="HMA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMA" evidence="2">
        <location>
            <begin position="7"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="73" mass="7878" checksum="92E1E0E19A55AF43" modified="2013-07-24" version="1">MFEKRIKKHLIIDGMLCRHCVASVTEALESLDGVTAKVNLKKQSATVTLTHPIADDTLIKVVTDAGFGVTNIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5V5N8</accession>
    <name>R5V5N8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">N(6)-L-threonylcarbamoyladenine synthase</fullName>
            <ecNumber evidence="2">2.3.1.234</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_01218</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenosine(37) in tRNA + L-threonylcarbamoyladenylate = AMP + H(+) + N(6)-L-threonylcarbamoyladenosine(37) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:37059"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.3.1.234"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.234" evidence="2"/>
    <dbReference type="EMBL" id="CBAR010000105">
        <property type="protein sequence ID" value="CCZ83444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01218"/>
    <dbReference type="EnsemblBacteria" id="CCZ83444">
        <property type="protein sequence ID" value="CCZ83444"/>
        <property type="gene ID" value="BN566_01218"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061711">
        <property type="term" value="F:N(6)-L-threonylcarbamoyladenine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000905">
        <property type="entry name" value="Gcp-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017861">
        <property type="entry name" value="KAE1/TsaD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735">
        <property type="entry name" value="PTHR11735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00814">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00789">
        <property type="entry name" value="OSIALOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TsaD" evidence="7">
        <location>
            <begin position="47"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="317" mass="34490" checksum="7024D8C7EB099A0F" modified="2013-07-24" version="1">MREFLGLDTSNYTTSCAIFDAENGTVRQAKKLLPVKSGMAGLRQSDAVFHHTRQLPEVIQTLLPNPPQNLTGIGVTTRPRNIEGSYMPCFLCGKTMAYGMATVTGVPVYETSHQIGHILAALYSAKKLSFLKAPFLAFHVSGGTTDCLLCEPDADLCLNITQVGTSLDLKAGQAIDRVGLMLQLQFPCGAALEQLAAASMKQYRTKPVIREGNCCLSGLENRCQAMLKQGEQPEDVARFCLTSVRDTVFEMTAFARKQYGQLPVLYAGGVMSNQWMREKLLSAGDVYFAEPAFSCDNAVGAAIYAALQAEGSETWQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5V679</accession>
    <name>R5V679_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 7">Phosphate propanoyltransferase</fullName>
            <ecNumber evidence="4 7">2.3.1.222</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_00151</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in 1,2-propanediol (1,2-PD) degradation by catalyzing the conversion of propanoyl-CoA to propanoyl-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>phosphate + propanoyl-CoA = CoA + propanoyl phosphate</text>
            <dbReference type="Rhea" id="RHEA:28046"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57392"/>
            <dbReference type="ChEBI" id="CHEBI:58933"/>
            <dbReference type="EC" id="2.3.1.222"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="7">Polyol metabolism; 1,2-propanediol degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the PduL family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.222" evidence="4 7"/>
    <dbReference type="EMBL" id="CBAR010000146">
        <property type="protein sequence ID" value="CCZ83624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00151"/>
    <dbReference type="EnsemblBacteria" id="CCZ83624">
        <property type="protein sequence ID" value="CCZ83624"/>
        <property type="gene ID" value="BN566_00151"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00621"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051144">
        <property type="term" value="P:propanediol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008300">
        <property type="entry name" value="PTAC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39453">
        <property type="entry name" value="PTHR39453"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06130">
        <property type="entry name" value="PTAC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010130">
        <property type="entry name" value="PduL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7 8" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR010130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="193" mass="20679" checksum="9E42C88FB2CD521E" modified="2013-07-24" version="1">MAKKFIVETSARHVHLTAEAFATLFGEGKELTVKKMLSQPGQFASEERVTIVGPKKSLPNVSILGPFRKANQVELSATDARSIGIVAPIRESGDVAGSGACKIVGPEGEIEISEGVIVAKRHIHLTPADAEEMGVKDKDVVWVRLDTDGRKAILGDVVCRVSDSYALAMHIDTDESNAVSAPRELYGEIVTVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>R5V6K9</accession>
    <name>R5V6K9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA topoisomerase 1</fullName>
            <ecNumber evidence="10">5.6.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">DNA topoisomerase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">topA</name>
        <name evidence="12" type="ORF">BN566_01489</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.</text>
            <dbReference type="EC" id="5.6.2.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the type IA topoisomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.1" evidence="10"/>
    <dbReference type="EMBL" id="CBAR010000161">
        <property type="protein sequence ID" value="CCZ83754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01489"/>
    <dbReference type="EnsemblBacteria" id="CCZ83754">
        <property type="protein sequence ID" value="CCZ83754"/>
        <property type="gene ID" value="BN566_01489"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003917">
        <property type="term" value="F:DNA topoisomerase type I (single strand cut, ATP-independent) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00186">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03363">
        <property type="entry name" value="TOPRIM_TopoIA_TopoI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00952">
        <property type="entry name" value="Topoisom_1_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000380">
        <property type="entry name" value="Topo_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003601">
        <property type="entry name" value="Topo_IA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023406">
        <property type="entry name" value="Topo_IA_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013497">
        <property type="entry name" value="Topo_IA_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013824">
        <property type="entry name" value="Topo_IA_cen_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013825">
        <property type="entry name" value="Topo_IA_cen_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013826">
        <property type="entry name" value="Topo_IA_cen_sub3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023405">
        <property type="entry name" value="Topo_IA_core_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003602">
        <property type="entry name" value="Topo_IA_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013498">
        <property type="entry name" value="Topo_IA_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005733">
        <property type="entry name" value="TopoI_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028612">
        <property type="entry name" value="Topoisom_1_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034149">
        <property type="entry name" value="TOPRIM_TopoI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42785">
        <property type="entry name" value="PTHR42785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01131">
        <property type="entry name" value="Topoisom_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01396">
        <property type="entry name" value="zf-C4_Topoisom"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00417">
        <property type="entry name" value="PRTPISMRASEI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00437">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00436">
        <property type="entry name" value="TOP1Bc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56712">
        <property type="entry name" value="SSF56712"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01051">
        <property type="entry name" value="topA_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00396">
        <property type="entry name" value="TOPOISOMERASE_I_PROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 10 12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0799">Topoisomerase</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Toprim" evidence="11">
        <location>
            <begin position="2"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with DNA" evidence="10">
        <location>
            <begin position="162"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="10">
        <location>
            <position position="302"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="32"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="138"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="154"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="304"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CCZ83754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="704" mass="78972" checksum="77BE72F4730B11AB" modified="2013-07-24" version="1">MSNLVIVESPAKAKTIQKYLGKDFDVIASMGHIRDLPKSKFGVDIENHFEPKYVDMKGKEDVIKQLKSHAAKCDKIYLATDPDREGEAISWHIAQMLHLDMQDNNRVAFNEITKSGVQAGMANPHRIDINLVNAQQARRVLDRIVGYQLSPFLWRKVRRGLSAGRVQSVAVRLVVDRENEIRAFQPQEYWTIDAKLSAAASRKVFSAKLAAVDGKKVDKTEISSKEQADAIVARLQEAPFVVKSVKKRVTKKHPSAPFITSTLQQEASYRLSFQSKRTMKVAQELYEGVEIQGMGAVGLITYMRTDSLRISDEAKKAAADYILSAYGESYLPPTPRVYKTKKNAQDAHEAIRPSMPELTPEQVKSSLTTDQYKLYRLIWERFIASQMADALMDTISVDIDADNCLFKASGSTMKFDGFTVLYEETRDNKGDKDKSENKNLPELHEGDALKLRALDGNQHFTQPPTRYSEASLIKAMEEYGIGRPSTYAPTITTITARNYVEREGKQLKPTVLGEVVTQLMKELFKNIVDVKFTAKMESDLDDVERGEKNWVQSLEQFYGDFSQTLKTAEEKMEGKRVKIPDEETDEVCEVCGKKMVIKIGRFGRFLACSGFPECTNTRKIVQEMPGCCPLCGGKMVLKKSKRGRSFYGCSNFPECNFMTWNIPVAEQCPQCGSTLFQKGGKSGKLICEKPGCGFEKNLSGGGQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>R5V6Z9</accession>
    <name>R5V6Z9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Arginine--tRNA ligase</fullName>
            <ecNumber evidence="9">6.1.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Arginyl-tRNA synthetase</fullName>
            <shortName evidence="9">ArgRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">argS</name>
        <name evidence="13" type="ORF">BN566_01608</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg)</text>
            <dbReference type="Rhea" id="RHEA:20301"/>
            <dbReference type="Rhea" id="RHEA-COMP:9658"/>
            <dbReference type="Rhea" id="RHEA-COMP:9673"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78513"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.19"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9 10">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.19" evidence="9"/>
    <dbReference type="EMBL" id="CBAR010000169">
        <property type="protein sequence ID" value="CCZ83879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01608"/>
    <dbReference type="EnsemblBacteria" id="CCZ83879">
        <property type="protein sequence ID" value="CCZ83879"/>
        <property type="gene ID" value="BN566_01608"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004814">
        <property type="term" value="F:arginine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006420">
        <property type="term" value="P:arginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00671">
        <property type="entry name" value="ArgRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00123">
        <property type="entry name" value="Arg_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001278">
        <property type="entry name" value="Arg-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005148">
        <property type="entry name" value="Arg-tRNA-synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036695">
        <property type="entry name" value="Arg-tRNA-synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035684">
        <property type="entry name" value="ArgRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008909">
        <property type="entry name" value="DALR_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956">
        <property type="entry name" value="PTHR11956"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03485">
        <property type="entry name" value="Arg_tRNA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05746">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00750">
        <property type="entry name" value="tRNA-synt_1d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01038">
        <property type="entry name" value="TRNASYNTHARG"/>
    </dbReference>
    <dbReference type="SMART" id="SM01016">
        <property type="entry name" value="Arg_tRNA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00836">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55190">
        <property type="entry name" value="SSF55190"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00456">
        <property type="entry name" value="argS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arg_tRNA_synt_N" evidence="12">
        <location>
            <begin position="10"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="DALR_1" evidence="11">
        <location>
            <begin position="442"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="9">
        <location>
            <begin position="136"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CCZ83879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="562" mass="62975" checksum="73B8770603F2C26B" modified="2013-07-24" version="1">MADCMKLAEKELYTLLLESVGRAVAAGTLPAEPMNTFKIEVPADKSHGDFAANIAMVNAKAFRMPPRKIAEAIFNELQLQGSSFEKAEIAGPGFMNFFLKRQWFSKTAQTVLAEGDDYGKTETGAGKRILVEFVSANPTGPMHVGNARGGALGDSLAEILNWAGYHAEREFYINDAGNQIEKFATSLEVRYLQLFDPSVPMPEDAYQGVDIIEHAEAFRDLYGDKYVNCDSKQRRDALVGYALPKNIAGLERDLKKYRIVYDNWFRESTLHKNGSVKHVVEELTKRGYTYEQDGAVWFKATEFGADKDFVLVRANGIPTYIVPDIAYHYDKLMTRHFDRAIDILGADHHGYVPRLKAALTAMGADANRLDVVLMQMVMLMRDGEVVKLSKRSGKAITLVTLLDEIPIDAARFFFNTREANSHFEFDLDLAVEQSSQNPVYYVQYAHARICSLLRNMEEEGVSVGEADLSVLNLLEQPQEIALIRHLAALPSRIDAAAASYDPSEMTKYATELATLFHKFYDACTVKHAEPELQQARLLLCQAAKQALRNTLRILKIDCPEKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5V762</accession>
    <name>R5V762_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ferredoxin:thioredoxin reductase</fullName>
            <ecNumber evidence="5">1.8.7.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Ferredoxin-thioredoxin reductase subunit B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN566_01668</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalytic subunit of the ferredoxin-thioredoxin reductase (FTR), which catalyzes the two-electron reduction of thioredoxins by the electrons provided by reduced ferredoxin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[thioredoxin]-disulfide + 2 H(+) + 2 reduced [2Fe-2S]-[ferredoxin] = [thioredoxin]-dithiol + 2 oxidized [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:42336"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.8.7.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="12">Heterodimer of subunit A (variable subunit) and subunit B (catalytic subunit). Heterodimeric FTR forms a complex with ferredoxin and thioredoxin.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ferredoxin thioredoxin reductase beta subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.8.7.2" evidence="5"/>
    <dbReference type="EMBL" id="CBAR010000174">
        <property type="protein sequence ID" value="CCZ83939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83939">
        <property type="protein sequence ID" value="CCZ83939"/>
        <property type="gene ID" value="BN566_01668"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016730">
        <property type="term" value="F:oxidoreductase activity, acting on iron-sulfur proteins as donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004209">
        <property type="entry name" value="FTR_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036644">
        <property type="entry name" value="FTR_bsu_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02943">
        <property type="entry name" value="FeThRed_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57662">
        <property type="entry name" value="SSF57662"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00026011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CCZ83939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="83" mass="9472" checksum="8EA5D9622E8169F9" modified="2013-07-24" version="1">MSVRLNPDADLVAAIREGLRKKGGYCPCRLERTEAYKCMCEEFRKQIADPEFEGYCHCMLYYKSNDAETGKDEKNGSHNSNKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7B1</accession>
    <name>R5V7B1_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01708</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000178">
        <property type="protein sequence ID" value="CCZ83979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83979">
        <property type="protein sequence ID" value="CCZ83979"/>
        <property type="gene ID" value="BN566_01708"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="118"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="262" mass="30142" checksum="E0EDF4919D4193B9" modified="2013-07-24" version="1">MITQMQNLTDAEHAILNQTWRGKKILSYLQAYGFDYEFCRSFRLTEGEKTGWMLLFNATLMICAEKPVALEETKTFVEMHLPFRIECPASFLPTLVTLPNYQKLRRTMFVLTPQPVSEEFQPEAVQKQPDLTAVYQILHEGFPNLLSYPLWLTDTSHRCRHGISQVLTYRDSTTVTILFDIENHVLVGQVATCAAARGSGYARDFLHWLANQLQQQGKYAVLYALDIRVSFYQEIGFEAADTEWVLERSDIEKEDVTKGALQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7L9</accession>
    <name>R5V7L9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Phosphate starvation-inducible protein PhoH predicted ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01820</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000186">
        <property type="protein sequence ID" value="CCZ84094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01820"/>
    <dbReference type="EnsemblBacteria" id="CCZ84094">
        <property type="protein sequence ID" value="CCZ84094"/>
        <property type="gene ID" value="BN566_01820"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003714">
        <property type="entry name" value="PhoH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02562">
        <property type="entry name" value="PhoH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PhoH" evidence="3">
        <location>
            <begin position="109"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="323" mass="36086" checksum="DF9A525B562D655E" modified="2013-07-24" version="1">MAEQILHMQDPAQWQALCGNFDENIRTLEQLYEVTIVCRDTEIKVNGAEAKAAQAAAVLQSLLQMLETGQPISLQTVRYAAMMVSEGEGQELQALADGGICLTATGKVVRARTVGQKRYVTAIQKNTIVFGIGPAGTGKTYLAVAMAVKAFKQHTVSRIILTRPAVEAGEKLGFLPGDLQNKVDPYLRPLYDALFEMMGAEAFQRNLERGVIEVAPLAYMRGRTLDHAFIILDEAQNTTSEQLKMFLTRLGEDSRMVITGDITQIDLPDKRKCGLLEAMQVLKHVNDIAIQEFTNKDVVRHRLVQDIIRAYETYHHRERKVEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7U2</accession>
    <name>R5V7U2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Glycosyl hydrolase family 48</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_01909</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01909"/>
    <dbReference type="EnsemblBacteria" id="CCZ84184">
        <property type="protein sequence ID" value="CCZ84184"/>
        <property type="gene ID" value="BN566_01909"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008810">
        <property type="term" value="F:cellulase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.870.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003343">
        <property type="entry name" value="Big_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023309">
        <property type="entry name" value="Endo-1-4-beta-glucanase_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027390">
        <property type="entry name" value="Endoglucanase_F_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000556">
        <property type="entry name" value="Glyco_hydro_48F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008964">
        <property type="entry name" value="Invasin/intimin_cell_adhesion"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02368">
        <property type="entry name" value="Big_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02011">
        <property type="entry name" value="Glyco_hydro_48"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00844">
        <property type="entry name" value="GLHYDRLASE48"/>
    </dbReference>
    <dbReference type="SMART" id="SM00635">
        <property type="entry name" value="BID_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="SSF48208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49373">
        <property type="entry name" value="SSF49373"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="141"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="7">
        <location>
            <begin position="1055"/>
            <end position="1129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ84184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="1132" mass="123838" checksum="276381783628A3B0" modified="2013-07-24" version="1">MWAFCERSAEIRKNSANSKKSEKTGTKYGQFIQTGFLNYGQNAEKTVSFVIRVDNQKGLGYNLNIKVDALCVDFVKKCECTTVTVVCSSFSACQSGHTGTVPMKGCVFWETHMPATKPFLLYILFLERGNFSMQFKKNKTVLAAILAAAMACSAIVPTAASAAGTRTKAEAYGDKTYAARFMSLYDDVITNGVQNGYMSSTSTVSGGLGVPYHSVETLCIEAPDYGHETTSEALSYLVWTAAMRDNIVNKANKGEITVKGEKDAASETVGDTAKAWKTMEATLIPDTQAGIMQKQQLSATYSDEWEQIELYPTDMLNGNNAVNPIHQYFTSAYGSDKGLYLMHWLADVDDWYGFGAGTSSQYKQQNVSGSFTMINTFQRGEQESCWETIPHACVEELKFGIQNGQEGSTRIGGMKGFFNTEQKVAQQYSYTNAPDAEDRAIQAVYAANRWGVADQSVDSKWGGSQKLDVLAAKMGDETRNNMFDKYYKTIGTTNKWDTSNSNGQYYLMNWYTSWGGALDGSWAWQIGCSHAHEFYQNPLAAYALAYDTNLSSNMKAQGAVEDFKKSFQTQMEYYLWLLSNDGVIAGGSTNSVNGRYEEHSKNVSGTSEFNKMVYVEHPVYADPGSNHWIGNQVWAVQRLAELYYVVKTQGDASGISVGGMDLTTALETILDKWTGWFLDNSILGKASGTITFEDYYEKYHATDGTGKTKFEIPDLSTVTDDGTSFSIPSSLIWSGEPNSWTGTYQENTNLKATIVGYGDGDLGCVSSLANTLIYYAAGRGVSASDLATGEASYKSSRGTKSTDMKDRAAQSLYLAKELLDREWNKYRDDIGLGVSDHNTNLTRLWETKLVLPNGQRTNGQGKTLAKGDYTGKMPNGDLIQDGVAFVDIRSNYKSDPMYLEAEKYYQQDGNTDNYYFTLHRFWHAGDIMMALGTMSEVYPDLTPDPTEDPDAPVVTPSEVTVKVGETKDLTVDQTGCDFKSDDDSIASVSKDGTITGVKEGKTTITVTNKDGKSTTVTVTVTAATTTEATTTSEATTTTGAKTTTTAGETTTVDPNADNIGDVNLDGVVDIADAVTLNKYLAGVVQLSDQALRNANCDQSPSDIDNIGDKDTTALVRFVLNVEGYQDLPFMGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7Z1</accession>
    <name>R5V7Z1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00013</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000197">
        <property type="protein sequence ID" value="CCZ84244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84244">
        <property type="protein sequence ID" value="CCZ84244"/>
        <property type="gene ID" value="BN566_00013"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004395118" evidence="2">
        <location>
            <begin position="25"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="210"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="241" mass="25732" checksum="43A17901A04D9D97" modified="2013-07-24" version="1">MIKKGLAAAAALMMLLTAAGCSDQDTSSSTADTASSVTTAETASTEVTTTVDIEALMPQEEELDPTLIPFQDIAFQVPAGWGNLNVENLVVWYPNDGSGSLTVQCFPEDELEITGTDEKDLLKNLGQNLAKGQTVFSEVWNELLGTDAYAITYSPETDASVDTAQKVNLSVLFYVNDLPYAMTFANYTGTSPVLKNSGAILNTVQLWDASDSTDTTDTTDTATDETDTETETEATTSESEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7Z6</accession>
    <name>R5V7Z6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC-type multidrug transport system ATPase component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_00018</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000197">
        <property type="protein sequence ID" value="CCZ84249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00018"/>
    <dbReference type="EnsemblBacteria" id="CCZ84249">
        <property type="protein sequence ID" value="CCZ84249"/>
        <property type="gene ID" value="BN566_00018"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="2"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="313"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ84249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="342" mass="38006" checksum="FF5D4450E98ADB71" modified="2013-07-24" version="1">MITVNHLTKRYGSHLAVDDISFTVQDGEIVGFLGPNGAGKSTTMNMLTGYISSSSGEALINDINILDNPIQAKREIGYLPELPPLYQEMTVMGYLRFVYDLKKCKLPRKAHLTEICNLCKISDVQDRIIKNLSKGYRQRVGLAQALVGNPPILILDEPTVGLDPKQMMEIRNLIKRLGKRHTVILSSHILSEIQAVCDRVIIINKGKLMADGAIDTLAQSMSQNHRLKVRLDGAEADVLQALRAIPDVAKVQQSTQLEAGLFDYEIEPKNNADLRKAVNAVAQEHGWNIYRMESGLLTLEDIFLKVTMGEWTPEQTKTEPDAAPKKPAPATKQPDTKQEEAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5V802</accession>
    <name>R5V802_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00183</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000200">
        <property type="protein sequence ID" value="CCZ84254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84254">
        <property type="protein sequence ID" value="CCZ84254"/>
        <property type="gene ID" value="BN566_00183"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008875">
        <property type="entry name" value="TraX"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05857">
        <property type="entry name" value="TraX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="165"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="195"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="230"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="252" mass="29137" checksum="6211A1763E47572F" modified="2013-07-24" version="1">MHAKISNKPYQIFNRDIIKYIAMFTMLLNHIATIFLSSGTWLCESFLAIGYFTAITMTYFLVEGYHYTHSKKIYILRLFVFALISEIPYCFAFTTNGIIEFYGFNMLFTLCICFGILYILDTIKNRFAKVCLIILAVIISLFCDWALLAPIFTLLFVWANNSKHRTKIAFCLSIVIFGTFNFLGGLGRFPFSTNILYALLSIVGMGMAAVCIVFFYNGKRIEKGKVFSKWFFYLFYPVHLLILGILRLVFIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>R5V8H3</accession>
    <name>R5V8H3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ATPase P-type (Transporting) HAD superfamily subfamily IC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN566_00321</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000225">
        <property type="protein sequence ID" value="CCZ84414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00321"/>
    <dbReference type="EnsemblBacteria" id="CCZ84414">
        <property type="protein sequence ID" value="CCZ84414"/>
        <property type="gene ID" value="BN566_00321"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="SSF81653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="SSF81660"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="SSF81665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-1278">Translocase</keyword>
    <keyword evidence="1 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="225"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="255"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="652"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="726"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="791"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="822"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="domain" description="Cation_ATPase_N" evidence="9">
        <location>
            <begin position="3"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ84414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="853" mass="91926" checksum="A3BF76C3F80FBB46" modified="2013-07-24" version="1">MMQGLTQQEAKNRLRQDGKNELAEAPKSKPVRMFLGQFRDVMVMILLAATMVSVLLGEWTDAITIILIVLLNAILGFIQEFRTERTLETLRQMTAPTAAVCRDGTWQTISAAELVRGDLIRLEAGDRVPADAALVTASGIAANESILTGESAAVSKTAGAETDTDNALHKKNVVYAGTAILRGSATACVIATGTKTQMGQISDLLHDVTVSQTPLQKRLAGLGKVVALLCIVVCILVFFAGVFRGEPIFDMLMTGITIAIAAIPEGLPATVTIALALAVSRMMKHQALVNRLHSVETLGCAGILCADKTGTITENDMTVTDLVISTAHFSVDQCIRQQGTAVSPEQHPALQTLLECCMLCTTAEHTPGQNPIGDPTETALLIAAEKAGFSRERCKQRYPLHHMEPFDSETKKMEVTVSYNGTERQYLKGAADRVLPLCTQLLHGTTVTTLTESFREEIRSAVQTLSERALRVLAFAYRESDGDWIFLGLSGMLDPPRESAKQAIRTCEQAKIQTVMITGDHKKTALAIAEQAGLLHGKKAMTGQELDALSDEQLAACIQEYAVFARVNPAHKLRLVRAYQKTGAIVAMTGDGVNDAPALKEADVGVAMGKSGTEVAKQAADVILLDDNLSTLVYAVEQGRCIYANIRKFVRYLLSCNIGEVITMLLGILMGMPVILLPTQLLLVNLVTDGLPAIALGMEQPEPDAMRQPPRKPEEPFFSGGLLQRIVFRGLLIGVCTLGAFSMALRLGGTLEAARTTALCTLILSQLIHVFECKSETKSLFHLPLWNNWKLLAAVAVSAGILFAAVESSALQMIFTTVPLSLPLWIVVAVSSCAIPICSAILFQITAKKKGER</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>R5VGP1</accession>
    <name>R5VGP1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Heavy metal-(Cd/Co/Hg/Pb/Zn)-translocating P-type ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_00006</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000001">
        <property type="protein sequence ID" value="CCZ82751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00006"/>
    <dbReference type="EnsemblBacteria" id="CCZ82751">
        <property type="protein sequence ID" value="CCZ82751"/>
        <property type="gene ID" value="BN566_00006"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="SSF81660"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="SSF81665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="30"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ82751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="186" mass="20000" checksum="AB9BF10EF24E1979" modified="2013-07-24" version="1">MADRWVTWIVVIALTAAALTWLLSGQIIRAVTILVVFCPCALVLATPTAIMAAIGNATKHGFLVREGDALERLAGVTKIMFDKTGTLTYGTSKVIAQKSLLPEWSDTELYALCAAVEQFSEHPLGKAIVQSYQQMHGAISQKAEQFQMWTGRGVSAQVSGVSVLAGNQKLLEEQQISVPKAAQADI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5VH25</accession>
    <name>R5VH25_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00668</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000015">
        <property type="protein sequence ID" value="CCZ82866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82866">
        <property type="protein sequence ID" value="CCZ82866"/>
        <property type="gene ID" value="BN566_00668"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="250" mass="27243" checksum="7802C0C5A0EB71F8" modified="2013-07-24" version="1">MIGIIEQGCVFCFDPSVGIIWPPDTNCLDPSIGGRIFIPVHSCLKNGFVFVSLLLQSILAVFHGDIQFCNLNITANREEFFHCGGIGRNAGKSVIVCIDVGLHPDSIQQALFIEGFAHAVNRIPLVIPVFVIIVVVKLAAFRCVFSGIGKGFFYKSIAAINLHPRGLSADILARFSCSAIVGSSVLVIADALIGNIIGIEIQFWILCLDCLKHIFNVGFHSLIHNGRRNPIAICIEIFFEEPDRGLTVPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>R5VHA8</accession>
    <name>R5VHA8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Enolase</fullName>
            <ecNumber evidence="9">4.2.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">2-phospho-D-glycerate hydro-lyase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">2-phosphoglycerate dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">eno</name>
        <name evidence="14" type="ORF">BN566_00731</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(2R)-2-phosphoglycerate = H2O + phosphoenolpyruvate</text>
            <dbReference type="Rhea" id="RHEA:10164"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="4.2.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="9">The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 9">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Cell surface</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Secreted</location>
        </subcellularLocation>
        <text evidence="9">Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 9">Belongs to the enolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.11" evidence="9"/>
    <dbReference type="EMBL" id="CBAR010000029">
        <property type="protein sequence ID" value="CCZ82931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00731"/>
    <dbReference type="EnsemblBacteria" id="CCZ82931">
        <property type="protein sequence ID" value="CCZ82931"/>
        <property type="gene ID" value="BN566_00731"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00187"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000015">
        <property type="term" value="C:phosphopyruvate hydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004634">
        <property type="term" value="F:phosphopyruvate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03313">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00318">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000941">
        <property type="entry name" value="Enolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020810">
        <property type="entry name" value="Enolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020809">
        <property type="entry name" value="Enolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020811">
        <property type="entry name" value="Enolase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902">
        <property type="entry name" value="PTHR11902"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00113">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03952">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001400">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00148">
        <property type="entry name" value="ENOLASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00002">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01192">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01193">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="SSF51604"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="SSF54826"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01060">
        <property type="entry name" value="eno"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00164">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="8 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Enolase_N" evidence="13">
        <location>
            <begin position="6"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Enolase_C" evidence="12">
        <location>
            <begin position="141"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="9 11">
        <location>
            <begin position="369"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="9 10">
        <location>
            <position position="207"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9 10">
        <location>
            <position position="342"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9 11">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9 11">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9 11">
        <location>
            <position position="290"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9 11">
        <location>
            <position position="317"/>
        </location>
    </feature>
    <feature type="binding site" description="covalent; in inhibited form" evidence="9">
        <location>
            <position position="342"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9 11">
        <location>
            <position position="393"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CCZ82931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="432" mass="46234" checksum="6B80AC06904850F9" modified="2013-07-24" version="1">MAYLEIKKVIGREIMDSRGNPTVEAEVLLADGTIGRGTAPSGASTGEFEALELRDGDKARYGGKGVTKAVENINTTINDVLVGLDASDIYAVDRAMLAADGTKDKSNLGANAILAVSIAAARAAAISLDIPLYRFLGGVSGNRLPVPMMNILNGGAHATNTVDTQEFMIMPVGAPTFKEALRWCAEVFHSLAKILKAKGLATSVGDEGGFAPNLSSDDETIETILEAVKAAGYEPGKDFMIAMDAASSEWKGEKGKGYYHQPKSGRDFTSDELIQHWADLVEKYPIISIEDALDEEDWEGWQKLTKELGDKVQLVGDDLFVTNTERLSKGISLGCGNSILIKLNQIGSVSETLEAIKMAHKAGYTAISSHRSGETADTTIADLAVALNTCQIKTGAPSRSERVAKYNQLLRIEEELGDAAVYPGMQAFNVKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5VHX7</accession>
    <name>R5VHX7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00928</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000063">
        <property type="protein sequence ID" value="CCZ83141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00928"/>
    <dbReference type="EnsemblBacteria" id="CCZ83141">
        <property type="protein sequence ID" value="CCZ83141"/>
        <property type="gene ID" value="BN566_00928"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13673">
        <property type="entry name" value="Acetyltransf_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="6"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="157" mass="17984" checksum="3371C0E6148C90C6" modified="2013-07-24" version="1">MQYIIKHFSELTTEELLEIYKLRVSVFVVEQKCPYQEVDDADRDSYHIWFQDEDGIQAYARVMDGGVTFAEPSIGRIIAKKRRCGLGSKIVSNGIQLAQERFHAKQIKIAAQTYAIPFYEQAGFRVISEPFLDVGVPHVLMLWEQPKESSINRGNVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>R5VI79</accession>
    <name>R5VI79_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Flavodoxin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01047</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the zinc metallo-hydrolase group 3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01047"/>
    <dbReference type="EnsemblBacteria" id="CCZ83266">
        <property type="protein sequence ID" value="CCZ83266"/>
        <property type="gene ID" value="BN566_01047"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016440">
        <property type="entry name" value="Rubredoxin-O_OxRdtase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005243">
        <property type="entry name" value="ROO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="2">
        <location>
            <begin position="245"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="386" mass="43258" checksum="20B3FECE7F2E61FB" modified="2013-07-24" version="1">MKEQKIADGIVYIGVNDKTIDLFESQYVVPNGVSYNSYCILDEKIAIMDTVDYRMSGEWLMNLDAVLQGKVPDYLIISHLEPDHAGTIAEFVAQYPQVTLVGNAKTFAMLPNFFDLKDQPKLTVAEGDTLSLGVHTLQFFMAPMVHWPEVMVEYEQKEKILFSADGFGKFGALDTEEPWEEEARRYYYNIVGKYGAPVQTLLKKAAKLDIAKICPLHGTILTENLGYYLEKYNLWSTYQPEEHGILIAHASIHGNTAKAAELLADLLLREGETVAVVDLARDDMAEAVANAFQYDRMVLAAASYDGGLFPPMEEFLLHLKAKSFQNRTVALIENGSWAPCAKKCMTNLLNEMKKITILEQAVTIRSTIKPETELQLQALAEAIRKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>R5VI85</accession>
    <name>R5VI85_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cystathionine gamma-synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01052</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 3">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the trans-sulfuration enzymes family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01052"/>
    <dbReference type="EnsemblBacteria" id="CCZ83271">
        <property type="protein sequence ID" value="CCZ83271"/>
        <property type="gene ID" value="BN566_01052"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00614">
        <property type="entry name" value="CGS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808">
        <property type="entry name" value="PTHR11808"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001434">
        <property type="entry name" value="CGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00868">
        <property type="entry name" value="CYS_MET_METAB_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="2">
        <location>
            <position position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR001434-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="422" mass="46600" checksum="97062BC640899500" modified="2013-07-24" version="1">MEQKQELPCESAWQFLQKNREAVKKMGKQTYGAEYGFQTRAVHTGNDVDAETGAIKRPITMANSYELPYDPSDLNWSSAGKNLYTRNGGSNQQYLQEKLASLEGGEDCIVLASGVAALSGLFFALLQSGDHVIFSSVTYIAVYRLLNELFNNKFQVETTIVDTSDLDAIRKAIRPNTKLIHIETPGNPTLTVSDIRAIAEIAHEHGILLSVDNTFASPYNQRPIELGADFTIESLTKYINGHGDAMGGAIIGKKEYLDLIRAQSQVNLGATISPFNAWLIMRGSVTLPLRMKQHNENAMKVANYLKTVPGVRFVAYPGLEDDPGHEIAKKQMQPGYGGVLSFGLKADHDTHNRFVSHLNVITSAVSLGHDESLIVFLGENDERQYLYPAAFHDGFFRFSVGIEDAEDIIADLQQALEQEGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJB4</accession>
    <name>R5VJB4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Lipolytic protein G-D-S-L family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00138</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000146">
        <property type="protein sequence ID" value="CCZ83611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00138"/>
    <dbReference type="EnsemblBacteria" id="CCZ83611">
        <property type="protein sequence ID" value="CCZ83611"/>
        <property type="gene ID" value="BN566_00138"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002105">
        <property type="entry name" value="Dockerin_1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013830">
        <property type="entry name" value="SGNH_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00404">
        <property type="entry name" value="Dockerin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13472">
        <property type="entry name" value="Lipase_GDSL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004395388" evidence="1">
        <location>
            <begin position="31"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="SGNH_hydro" evidence="2">
        <location>
            <begin position="42"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="369" mass="39299" checksum="E795D5CE6A8BE86B" modified="2013-07-24" version="1">MKNRKTGKAIAAAAMLTIAAMTMPMQTVSAEDIVDAPYNVVVLGDSISTGYGLEDSSKSYVSVLEHHMNQKVTNLAKDGTTSSELLQSLQGDSTMQSAVAGADIIVVTIGANDILQPVLNNDVVKVDDYDNVYDLANAIKDNQIAFQKYLRATMPTAVANANTNIDSIILQLKSTNDHAKLIFQTVYDPLSVDQDDTGLSTNALSMLSAFSNGQMYQYLNGSANGGTYILVGLNQNLQTHAQNGEIYLADVYSAFLHHAWTNVNIANADVHPTATGHAAIANLLIESGYFPSVANIDGDIDGDEKIDVSDAVAVLTEYARIAAGNEAQFNPAQKKSADVNEDGMLDVSDAVGILIYYAKQASGQTPSFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJC3</accession>
    <name>R5VJC3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Sodium/proline symporter</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Proline permease</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN566_00148</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the sodium-dependent uptake of extracellular L-proline.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-proline(in) + Na(+)(in) = L-proline(out) + Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:28967"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000146">
        <property type="protein sequence ID" value="CCZ83621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00148"/>
    <dbReference type="EnsemblBacteria" id="CCZ83621">
        <property type="protein sequence ID" value="CCZ83621"/>
        <property type="gene ID" value="BN566_00148"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005298">
        <property type="term" value="F:proline:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031402">
        <property type="term" value="F:sodium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015824">
        <property type="term" value="P:proline transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11475">
        <property type="entry name" value="SLC5sbd_PutP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011851">
        <property type="entry name" value="Na/Pro_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018212">
        <property type="entry name" value="Na/solute_symporter_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00813">
        <property type="entry name" value="sss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00456">
        <property type="entry name" value="NA_SOLUT_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00457">
        <property type="entry name" value="NA_SOLUT_SYMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0915">Sodium</keyword>
    <keyword evidence="8" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="8" id="KW-0769">Symport</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="5"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="68"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="125"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="163"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="243"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="282"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="340"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="393"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="417"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="448"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="480"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00033708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ83621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="518" mass="55770" checksum="4C68B2058DB0493E" modified="2013-07-24" version="1">MKSNLICMLITIVAYLILMIVVGILASKKNKDTSDFYLGGRKLGPIVTAMSAEASDMSSYLLMGLPGLALLTGFADVGWTVIGLAIGTYLNWLFVAKRLRIYSQHNGSITIPDFFSNRYHDNKHFLMGISAIIILIFFVPYTASGFSACGKLFQTLFGMNYHAAMILSAVIIIAYTSIGGFLAASTTDLLQSIIMTVALVMVVVFGIVKAGGIDVVMENAKALPGYLSFNHTYDITTNSAKPYNILTICSTLAWGLGYFGMPHILLRFMAIEDSRKIKTSRRIASIWVVISMGIAILIGFIGKTLMTNSTISVLGDNPDNFSAESETVIMKIATLISEHGVLAALFAGLIFAGILACTMSTSDSQLLAASSSMSENLLQGVFGIKLSEKTAMLAARAVLLIIAVISIGIAWNPDSSVFHIVSFAWAGFGATFGPVMLCALFWKRSNKWGALAGLVAGGVMVFVWKFLVRPLGGAWDLYELLPAFIVALAAIVIVSLLTKAPDNELVEEFEAVQAECKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJH3</accession>
    <name>R5VJH3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01417</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000148">
        <property type="protein sequence ID" value="CCZ83681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01417"/>
    <dbReference type="EnsemblBacteria" id="CCZ83681">
        <property type="protein sequence ID" value="CCZ83681"/>
        <property type="gene ID" value="BN566_01417"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="54" mass="6169" checksum="31A6D621FB106EE7" modified="2013-07-24" version="1">MTTPLFLLRCVQIGLSISDLDFLTIGLVNDLFTEKENDGYPYSYQATQADFDKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJH8</accession>
    <name>R5VJH8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPF0102 protein BN566_01422</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01422</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the UPF0102 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000153">
        <property type="protein sequence ID" value="CCZ83686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01422"/>
    <dbReference type="EnsemblBacteria" id="CCZ83686">
        <property type="protein sequence ID" value="CCZ83686"/>
        <property type="gene ID" value="BN566_01422"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00048">
        <property type="entry name" value="UPF0102"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003509">
        <property type="entry name" value="UPF0102"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34039">
        <property type="entry name" value="PTHR34039"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02021">
        <property type="entry name" value="UPF0102"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="SSF52980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="126" mass="14339" checksum="13EB69DB952DAE23" modified="2013-07-24" version="1">MTPSEAGRRGETAVCQYLLQRGYTIRKRNYRIRGGEIDIIAQKDEILAFVEVKSRRQSEGFPLEYLSPQQQLRIVKASLQYCAAENPDWALQPRYDAAAVVTERGQIVSIEYVENAFDASVLDAIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJX2</accession>
    <name>R5VJX2_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Histidine--tRNA ligase</fullName>
            <ecNumber evidence="9">6.1.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Histidyl-tRNA synthetase</fullName>
            <shortName evidence="9">HisRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">hisS</name>
        <name evidence="11" type="ORF">BN566_01576</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>ATP + L-histidine + tRNA(His) = AMP + diphosphate + H(+) + L-histidyl-tRNA(His)</text>
            <dbReference type="Rhea" id="RHEA:17313"/>
            <dbReference type="Rhea" id="RHEA-COMP:9665"/>
            <dbReference type="Rhea" id="RHEA-COMP:9689"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78527"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.21"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3 9">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.21" evidence="9"/>
    <dbReference type="EMBL" id="CBAR010000166">
        <property type="protein sequence ID" value="CCZ83841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01576"/>
    <dbReference type="EnsemblBacteria" id="CCZ83841">
        <property type="protein sequence ID" value="CCZ83841"/>
        <property type="gene ID" value="BN566_01576"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004821">
        <property type="term" value="F:histidine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006427">
        <property type="term" value="P:histidyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00773">
        <property type="entry name" value="HisRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00859">
        <property type="entry name" value="HisRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00127">
        <property type="entry name" value="His_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015807">
        <property type="entry name" value="His-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041715">
        <property type="entry name" value="HisRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004516">
        <property type="entry name" value="HisRS/HisZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033656">
        <property type="entry name" value="HisRS_anticodon"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43707">
        <property type="entry name" value="PTHR43707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13393">
        <property type="entry name" value="tRNA-synt_His"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001549">
        <property type="entry name" value="His-tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00442">
        <property type="entry name" value="hisS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II" evidence="10">
        <location>
            <begin position="25"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ83841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="434" mass="48458" checksum="69EE56DD98ED20DD" modified="2013-07-24" version="1">MALKITRPQGTEDITPENISKWYTVEQVARDTAKCFGFHEMRIPTFESTDLFLRSVGETTDVVQKEMYTVKAKETEFTLRPEGTAGTIRSMLQNGMLNAALPQKVFYILSCFRHERPQAGRLREFHQFGVEMAGSADAAADAEIISLAKSILDRIGLKNIQLYINSIGCPTCRANYHKALRAYFAAYEDDLCDTCKSRLEKNPMRILDCKSPICSKIAADAPIILDYLCDECSEHFQTLQSHLKALQIDFTINPKIVRGLDYYTKTVFEFVTTEIGAQGTVCGGGRYDGLIGQLGGADTPALGFAMGLERLLLTMQQQGCNFLETPPCDLYIAPMDAEALPKAMQLAQAVRESGYQAEYDLMHRGLKAQMKYANKIHATYVLVLGGNELEQQSAKLKQMETGEQIAIPLDDRFAEAFDNIAISHMFQNTLQEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>R5VK21</accession>
    <name>R5VK21_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Copper-exporting P-type ATPase</fullName>
            <ecNumber evidence="3">7.2.2.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Copper-exporting P-type ATPase A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Cu(+)-exporting ATPase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">BN566_01620</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + Cu(+)(in) + H2O = ADP + Cu(+)(out) + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:25792"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:49552"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.8" evidence="3"/>
    <dbReference type="EMBL" id="CBAR010000170">
        <property type="protein sequence ID" value="CCZ83891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01620"/>
    <dbReference type="EnsemblBacteria" id="CCZ83891">
        <property type="protein sequence ID" value="CCZ83891"/>
        <property type="gene ID" value="BN566_01620"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140581">
        <property type="term" value="F:P-type monovalent copper transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017969">
        <property type="entry name" value="Heavy-metal-associated_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006122">
        <property type="entry name" value="HMA_Cu_ion-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="SSF55008"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="SSF81653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="SSF81665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00003">
        <property type="entry name" value="TIGR00003"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01047">
        <property type="entry name" value="HMA_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0186">Copper</keyword>
    <keyword evidence="8" id="KW-0187">Copper transport</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="16" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004407133" description="Copper-exporting P-type ATPase" evidence="16">
        <location>
            <begin position="26"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="129"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="207"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="358"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="391"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="699"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="727"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="17">
        <location>
            <begin position="3"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="17">
        <location>
            <begin position="771"/>
            <end position="834"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU362081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CCZ83891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="837" mass="88727" checksum="FF1316CB4850EE0B" modified="2013-07-24" version="1">MKKERYAVTGMTCSACSAHVQKAVAAVPGVHSVQVNLLQNNMTVEFEEAETTPETIFAAVKKAGYGAAPLSESGTTAAPTGGTSIVSAHAERNRLIWSIVFLIPLFYIGMGHMINLFPLPSVLKGHSSMMLIALIELVLVTPILFLNRKYFIKGTTTLLHGAPTMDTLIALGSAVSFCYSLYATFAIAYDMGQMDMDAAHSHMNQLYYESAGMILTLISLGKFLEARSKAKTTDAISSLVKLTPQTALVRRNGVEQEIPTASIQVGDTILVKTGAAIPADGTVISGQGSVDESALTGESLPVEKQVGDRILSATTCRSGYLEYRAEQVGADTTLSQIIRLVEEAGSSKAPIARLADKISGVFVPIVISIAIVTLIVWLLTGNPFSMALKAAVSVLVISCPCALGLATPTAIMVGTGQGAKNGILIKSAESLETAHSVKAVVLDKTGTLTEGVPTVMDVLPEEGVSAETLLSVAYALEQPSEHPLAAAIVTYCKQQNIPLQPAEQFAQTAGQGVSGICNGMHCFGGNQKMMEANHISLPSTEKQEAFAKAGKTPLYFAADGKLLGTLTAADPIRQTSRTAVAEFQRMGLDVILLTGDNRLTAEAIARQAGITHVIADVLPQDKAMQVKQLQADGKKTAMIGDGINDAPALTQADIGIAIGAGTDAAIDSADIVLMRNDLQDAVTAIQLSRATIRNIKENLFWAFIYNLIGIPIAAGVFYPIFGWEMNPMIGAAAMSFSSVFVVSNALRLRRFRPFGKSSSKKPDTTPVANGEIVIQIKGMMCEHCVAAVTKALQSVSGVTEMRVVLSENRAYCKGTPTDAELRTAIQNAGYQVKKIIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>R5VK27</accession>
    <name>R5VK27_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Elongation factor G</fullName>
            <shortName evidence="7">EF-G</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">fusA</name>
        <name evidence="9" type="ORF">BN566_01625</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000171">
        <property type="protein sequence ID" value="CCZ83896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01625"/>
    <dbReference type="EnsemblBacteria" id="CCZ83896">
        <property type="protein sequence ID" value="CCZ83896"/>
        <property type="gene ID" value="BN566_01625"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16262">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01434">
        <property type="entry name" value="EFG_mtEFG1_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03713">
        <property type="entry name" value="EFG_mtEFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00054_B">
        <property type="entry name" value="EF_G_EF_2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009022">
        <property type="entry name" value="EFG_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035649">
        <property type="entry name" value="EFG_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004540">
        <property type="entry name" value="Transl_elong_EFG/EF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005517">
        <property type="entry name" value="Transl_elong_EFG/EF2_IV"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03764">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00889">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="SSF54980"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00484">
        <property type="entry name" value="EF-G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7 9" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="6 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="8">
        <location>
            <begin position="8"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="17"/>
            <end position="24"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="81"/>
            <end position="85"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="135"/>
            <end position="138"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ83896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="691" mass="76680" checksum="1B1B32BEC3F88EF9" modified="2013-07-24" version="1">MARDCSLENTRNIGIMAHIDAGKTTTTERILFYTGINHKIGETHEGASTMDWMEQEKERGITITSAATTAYWGGHRINIIDTPGHVDFTVEVERSLRVLDGSVTVFCAKGGVEPQSETVWRQADKYQVPRMAYVNKMDIMGANFYRVVEMMHDRLKCNAVPIQLPIGAESDFKGIIDLVEMKAYVYYDDLGKDIRVEEIPDDMKEKAEEYRASLMEHVAEQDDALMEKYFADEEITIDEIKACIRKSTIANTMVPVVCGTSYKNKGVQKLLDAIVDYMPSPLDVPSIKGTNPETGEEEFRHSSDTEPFAALAFKIATDPFVGKLCFFRVYSGTVDAGSTVLNATKDNRERMGRILQMHSNHRKDIETVYAGDIAAAVGLKNTTTGDTLCDEKNPIILESMEFPEPVIQLAIEPKTKAGQEKMGIALAKLAEEDPTFRTYTDEETGQTIIAGMGELHLDIIVDRLLREFKVEANVGAPQVSYKETIKGEADVDYKYKKQSGGSGQYGHVKIRVRPNESGKGYEFINKIVGGSIPKEYIPAVDAGIQGAMQAGILAGYQVVDVSVELYDGSYHEVDSSEMAFKIAGSIAFKEAMRQAQPVLMEPIMKVSVIVPDEYVGTVIGDLSSRRGQIQGQETRTGTVQVDALVPLSEMFGYTNDLRSNTQGRGQYTMEPHSYQEVPKNIAEKIMAARKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5VKC6</accession>
    <name>R5VKC6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01700</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000177">
        <property type="protein sequence ID" value="CCZ83971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01700"/>
    <dbReference type="EnsemblBacteria" id="CCZ83971">
        <property type="protein sequence ID" value="CCZ83971"/>
        <property type="gene ID" value="BN566_01700"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.4300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024232">
        <property type="entry name" value="SpoIIIAH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038503">
        <property type="entry name" value="SpoIIIAH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12685">
        <property type="entry name" value="SpoIIIAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="80" mass="8485" checksum="80712CE902D18D6F" modified="2013-07-24" version="1">MVTDAIAAVETSKLIESESNIESLIKSQGYADCIVYLDGDSAKVVVKTEGLDSAQAAAIKEAILGEVTLPAENIRIFEVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VKR4</accession>
    <name>R5VKR4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01832</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000187">
        <property type="protein sequence ID" value="CCZ84106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84106">
        <property type="protein sequence ID" value="CCZ84106"/>
        <property type="gene ID" value="BN566_01832"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="88" mass="9440" checksum="43920FADA6D9417C" modified="2013-07-24" version="1">MWKCFKNEKLWCAIGGAAALVIGKKILTAETTRKLAVSGLAKGMKLQSDAKATFQNMKDEASDICCDAKEEAGLADIAEEAATEEETK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5VQA9</accession>
    <name>R5VQA9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">SCP-like extracellular</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00572</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000007">
        <property type="protein sequence ID" value="CCZ82767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00572"/>
    <dbReference type="EnsemblBacteria" id="CCZ82767">
        <property type="protein sequence ID" value="CCZ82767"/>
        <property type="gene ID" value="BN566_00572"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002105">
        <property type="entry name" value="Dockerin_1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00404">
        <property type="entry name" value="Dockerin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="SSF55797"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004395342" evidence="1">
        <location>
            <begin position="40"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="2">
        <location>
            <begin position="38"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ82767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="250" mass="27006" checksum="63B5B953302533FC" modified="2013-07-24" version="1">MKQKITAVRTILLRGFACILSTVFGISFLLNGSAFSASAAELGDVDGDGSVTIQDACWILETYAKISAGIPTEVSSDLKVTADVDEDGVISVQDAVMVLQYYAKQAAGITVSWEILRMSAAEQLAWDRSQKVLELVNENRVENGLAELSFNKELYQAASIRAEELATSFSHSRPNGKSAFSVLDDLEIFYMCAGENIAAGFNTPKTAITAWMHSPGHRANILGTDYTSAAVGVYVSSDETCYWCLMLIGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5VQM4</accession>
    <name>R5VQM4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Precorrin-6A reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00679</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000020">
        <property type="protein sequence ID" value="CCZ82877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00679"/>
    <dbReference type="EnsemblBacteria" id="CCZ82877">
        <property type="protein sequence ID" value="CCZ82877"/>
        <property type="gene ID" value="BN566_00679"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016994">
        <property type="term" value="F:precorrin-6A reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003723">
        <property type="entry name" value="Precorrin-6x_reduct"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36925">
        <property type="entry name" value="PTHR36925"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02571">
        <property type="entry name" value="CbiJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51014">
        <property type="entry name" value="COBK_CBIJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ82877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="231" mass="24893" checksum="067C5D9B925F0E3F" modified="2013-07-24" version="1">MKQRKIAVIAGTSDATDWIRSLPETDSVTAFVATPYGKEILAGTNCTVHVGRLDAAGFAEALTGFDEVVDASHPFAVVVTETVKAVCQAKQIPYTRISRPTLHYDYEKLHIVSSKEAAADWLCSCTGNLFFTVGIQTMPFYAAAVSDFSHRAFARVLDTAASRQAAAAIPATFWFAMPPYSIAETVAYLQEHQISVLVSKDSGARGGVPEKIQAAKQVGIPVLLIQSPEQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5VRQ8</accession>
    <name>R5VRQ8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">L-serine deaminase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN566_01023</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8 9">
            <text>L-serine = NH4(+) + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:19169"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="EC" id="4.3.1.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 9">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="8">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8 9">Belongs to the iron-sulfur dependent L-serine dehydratase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000081">
        <property type="protein sequence ID" value="CCZ83242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01023"/>
    <dbReference type="EnsemblBacteria" id="CCZ83242">
        <property type="protein sequence ID" value="CCZ83242"/>
        <property type="gene ID" value="BN566_01023"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003941">
        <property type="term" value="F:L-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029009">
        <property type="entry name" value="ASB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004643">
        <property type="entry name" value="Fe-S_L-Ser_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005131">
        <property type="entry name" value="Ser_deHydtase_bsu"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01842">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03315">
        <property type="entry name" value="SDH_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036692">
        <property type="entry name" value="SDH_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143548">
        <property type="entry name" value="SSF143548"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00719">
        <property type="entry name" value="sda_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="8 9" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="5 8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="6 8 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 8 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACT" evidence="10">
        <location>
            <begin position="146"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR036692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU366059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ83242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="222" mass="23792" checksum="269C3CADDFBD99C9" modified="2013-07-24" version="1">MHIFDIIGPVMIGPSSSHTAGAAKIGYVTRKLLGERPVQAEILLHGSFSATGSGHGTDRAIVAGLLGYKPDDERLPNSFAEAEKAGLSFQLTHGVIRNAHPNTAKIHAIGENGNELEVVGASIGGGRIKICEIDGICTNFSGEYNTIIVHNMDTPGHVAQVTTTLAQKNVNIATMQLYRNTQGGYAVMILECDDIIPKNTLEWLQDLDGIIKVTSYNVEEEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>R5VRS4</accession>
    <name>R5VRS4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8 10">FAD:protein FMN transferase</fullName>
            <ecNumber evidence="2 8 10">2.7.1.180</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Flavin transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN566_01043</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Flavin transferase that catalyzes the transfer of the FMN moiety of FAD and its covalent binding to the hydroxyl group of a threonine residue in a target flavoprotein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8 10">
            <text>FAD + L-threonyl-[protein] = AMP + FMN-L-threonyl-[protein] + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36847"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11061"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:74257"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.1.180"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="9">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="9">Magnesium. Can also use manganese.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8 10">Belongs to the ApbE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.180" evidence="2 8 10"/>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01043"/>
    <dbReference type="EnsemblBacteria" id="CCZ83262">
        <property type="protein sequence ID" value="CCZ83262"/>
        <property type="gene ID" value="BN566_01043"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017013">
        <property type="term" value="P:protein flavinylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024932">
        <property type="entry name" value="ApbE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003374">
        <property type="entry name" value="ApbE-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30040">
        <property type="entry name" value="PTHR30040"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02424">
        <property type="entry name" value="ApbE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006268">
        <property type="entry name" value="ApbE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143631">
        <property type="entry name" value="SSF143631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0274">FAD</keyword>
    <keyword evidence="4 8 10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 8 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="5 8 10" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005970577" description="FAD:protein FMN transferase" evidence="10">
        <location>
            <begin position="28"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR006268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR006268-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ83262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="364" mass="38930" checksum="5039BF32C196A7C1" modified="2013-07-24" version="1">MLRKWMLALCFCLGIFGCGCSETTSNESNTTSSEAVTATTQSADEKTTRDLFAMDTYITLTAYGNNAEAGISAAEACIQDLDARLSTGNSESEIAKLNATGSGTLSEDTSAILQEAITLYQDTDGAFNPLMYPIMQAWGFPTHEYRVPEDSEIQSLLQLTDPSKMQFSAETGAVSFSVSGMELDLGGIAKGYTSSAMMDCFRSNGVTSGMVSLGGNVQTIGTKPDGSLWKVAIQNPTAEEDADFLGILETKDCAVITSGGYERYFEENGVRYHHIMDPATGKPAESDLTSVTIVSENGTLADGLSTALFVMGLDKAIAYWQAHASEFDAVLCTEDETLYVTEGIQDAFSANYPCEIIQQKGAES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>R5VSH3</accession>
    <name>R5VSH3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Stage 0 sporulation protein A homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN566_01246</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000114">
        <property type="protein sequence ID" value="CCZ83477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01246"/>
    <dbReference type="EnsemblBacteria" id="CCZ83477">
        <property type="protein sequence ID" value="CCZ83477"/>
        <property type="gene ID" value="BN566_01246"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3 8 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Response regulatory" evidence="9">
        <location>
            <begin position="3"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="10">
        <location>
            <begin position="126"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="8">
        <location>
            <begin position="126"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="7">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ83477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="223" mass="25494" checksum="1581D3B7792447AE" modified="2013-07-24" version="1">MYRIFIVEDDSTMAAVMKKQIESWGHSVHVATQFSDILSEFTAYDPHMVLMDVMLPFFNGYHWCSEIRKISNVPVMFISSVSDNMNIVMAMNMGGDDFIAKPVDLTVMMAKIQAMLRRTYDMGGKIPVLEHQGAVLNLNDAVLTYQGKQLELTKNEFRILQTLLEQKGKIVSRETLMMKLWQVDSYVEENTLTVNVTRLRKKLEQIGLGGWIRTKVGSGYLIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>R5VSL4</accession>
    <name>R5VSL4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP-dependent 6-phosphofructokinase</fullName>
            <shortName evidence="10">ATP-PFK</shortName>
            <shortName evidence="10">Phosphofructokinase</shortName>
            <ecNumber evidence="10">2.7.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Phosphohexokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">pfkA</name>
        <name evidence="12" type="ORF">BN566_01266</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 1,6-bisphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 10">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer or homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.11" evidence="10"/>
    <dbReference type="EMBL" id="CBAR010000118">
        <property type="protein sequence ID" value="CCZ83497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01266"/>
    <dbReference type="EnsemblBacteria" id="CCZ83497">
        <property type="protein sequence ID" value="CCZ83497"/>
        <property type="gene ID" value="BN566_01266"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00182"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003872">
        <property type="term" value="F:6-phosphofructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047334">
        <property type="term" value="F:diphosphate-fructose-6-phosphate 1-phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01976">
        <property type="entry name" value="Phosphofructokinase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022953">
        <property type="entry name" value="ATP_PFK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012003">
        <property type="entry name" value="ATP_PFK_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015912">
        <property type="entry name" value="Phosphofructokinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000023">
        <property type="entry name" value="Phosphofructokinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012829">
        <property type="entry name" value="Phosphofructokinase_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035966">
        <property type="entry name" value="PKF_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00365">
        <property type="entry name" value="PFK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000532">
        <property type="entry name" value="ATP_PFK_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00476">
        <property type="entry name" value="PHFRCTKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53784">
        <property type="entry name" value="SSF53784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00433">
        <property type="entry name" value="PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 10" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7 10 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PFK" evidence="11">
        <location>
            <begin position="4"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="10">
        <location>
            <begin position="137"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="10">
        <location>
            <begin position="284"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="77"/>
            <end position="78"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="114"/>
            <end position="117"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="site" description="Important for substrate specificity; cannot use PPi as phosphoryl donor" evidence="10">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CCZ83497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="360" mass="38818" checksum="ED1591B88C5EABFC" modified="2013-07-24" version="1">MKRRIGILTSGGDCPGLNATIRGVAKACYQMLGTENVEIVGISDGYYGLIHNICKEMKPDDFSGILTRGGTILGTKRQPFKMMKVIGDDNIDKVKNMKKTYQSQKLDCLLTLGGNGTHKTSQLLSEEGLNVIGLPKTIDNDIYGTDVTFGFHTAVDIATDAIDRLHTTANSHSRILLCEIMGNKAGWLTLNAGIAGGADIILIPEIPYDIDKVCKSVLKRSESGKNFSIIAVAEGCFDKEEAKLKKKERARLRAEAGVITATNRIAAQIQQKTQLEARVCVPGHMLRGGSPSAYDRILATQFGVYAAQLIKEEHYGMTVAKVGGTITANPLAEIAGKTKFVTPDDQLVATARNLGISFGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5VSM7</accession>
    <name>R5VSM7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">L-threonine dehydratase catabolic TdcB</fullName>
            <ecNumber evidence="5">4.3.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Threonine deaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_01291</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2-ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>L-threonine = 2-oxobutanoate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:22108"/>
            <dbReference type="ChEBI" id="CHEBI:16763"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="EC" id="4.3.1.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 5">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid degradation; L-threonine degradation via propanoate pathway; propanoate from L-threonine: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">In the native structure, TdcB is in a dimeric form, whereas in the TdcB-AMP complex, it exists in a tetrameric form (dimer of dimers).</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 5">Belongs to the serine/threonine dehydratase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.19" evidence="5"/>
    <dbReference type="EMBL" id="CBAR010000121">
        <property type="protein sequence ID" value="CCZ83522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01291"/>
    <dbReference type="EnsemblBacteria" id="CCZ83522">
        <property type="protein sequence ID" value="CCZ83522"/>
        <property type="gene ID" value="BN566_01291"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00052">
        <property type="reaction ID" value="UER00507"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004794">
        <property type="term" value="F:L-threonine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070689">
        <property type="term" value="P:L-threonine catabolic process to propionate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04886">
        <property type="entry name" value="ACT_ThrD-II-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044561">
        <property type="entry name" value="ACT_ThrD-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005789">
        <property type="entry name" value="Thr_deHydtase_catblc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01127">
        <property type="entry name" value="ilvA_1Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACT" evidence="6">
        <location>
            <begin position="325"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ83522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="398" mass="42886" checksum="5B78BE27A7A7DAC7" modified="2013-07-24" version="1">MLTLDKIYKASHVLRSVIRKTDLIHAPLVNPDSEIYLKTENLQITGSFKVRGAYYMMSQLTEEEKARGVIACSAGNHAQGVALAAAKNHIKSLICLPDGAPISKVEATKSYGAEVCMVNGVYDDAYQKALELKEEKNYTFVHPFDNEDVIAGQGTIGLEILEQMPDVEAVIVPIGGGGLISGVAFAIKNLNPNIKVYGVQASGAPSMRNSIRDGKIECLDHVSTIADGIAVKEPGEHTFQYVSQYVDEIVTVTDDEISAAILALIERQKLIAEGAGAASVAAAMFNKVPIQGKKTVCLVSGGNIDVTILSRVIKRGLLMSGRTSMLNIELLDKPGQLKLVSEIIADMGGNVISIHHERASEGSDVNGCFLRITMETRDFNHIQQIRDALTKAGLKLVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>R5VSV9</accession>
    <name>R5VSV9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ATPase components of ABC transporters with duplicated ATPase domains</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_00149</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000146">
        <property type="protein sequence ID" value="CCZ83622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00149"/>
    <dbReference type="EnsemblBacteria" id="CCZ83622">
        <property type="protein sequence ID" value="CCZ83622"/>
        <property type="gene ID" value="BN566_00149"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032781">
        <property type="entry name" value="ABC_tran_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12848">
        <property type="entry name" value="ABC_tran_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="320"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="533" mass="60598" checksum="539D74CF380F43A3" modified="2013-07-24" version="1">MITVSNVSLQFGGDTLFKNVNLQFNAGNCYGVIGANGAGKSTFLKILCGVLPPTTGEVTIPKELRMSVLKQDHFAYDAYTVLDTVIMGNERLYAIMREKDALYAKPDFSEEDGNHAAELEAEFAELNGWEAESDVSKLLQGLGLSEDLLYQTMGDLSGNEKVKILLAQALFGNPDIILMDEPTNHLDIDAITWLEDFLSEYFGTVIVVSHDRHFLNNVCTHIVDIDYTQIKMYVGNYEFWYESSQMMQRMIRQQNKKTEEKIKELKTFIERFSANKSKSRQATSRRKLLDNLTVEQLPASSRRYPFIGFDMDRELGKEVLQVNGISKTVDGVKLLNNVSFTLSRTDKVAFIGESEQAITMLFKILMEEEQPDEGTFKWGVSTSTSYFPVDNSVYFNDCDLSILDWIRQYSKTDETETYLRGFLGRMLFSGDDIYKPVKVLSGGEKVRCMFSRMMLYHSNVLLLDRPTNHLDLESITAVNNGLTAFKGVVILSSHDHEILQTVANRIIEITPDGCLDRQGTYEEFLDWKKENQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5VSX4</accession>
    <name>R5VSX4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00164</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000146">
        <property type="protein sequence ID" value="CCZ83637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00164"/>
    <dbReference type="EnsemblBacteria" id="CCZ83637">
        <property type="protein sequence ID" value="CCZ83637"/>
        <property type="gene ID" value="BN566_00164"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="322" mass="35434" checksum="00B7451A6C69B880" modified="2013-07-24" version="1">MLPTLYGKKSLQTTLSRMASGERLAQSFLFYGDAGLGRKTVAHWFSALLLCEHPENGQPCGHCKSCHNLQKDCHPDLILVPHSGKLGGFSVETVRQICSSVSIPPNNGNRKVYLFLDCDQMDDRAQNLLLKQLEEPPAYAFFLFTASAKDTLLSTIRSRILSFGLIPCQPSEVQEAATALGIPDADFQRFPGNIGQTLAYATDSNLQTAVARIEDSIQALQQHNEYAFLKAVAAIGKDRPQARFFLQQLRLYVRDVLVLHNDPSAATCSCAPQLAKQAARQTAQRQGLLQFETIRKAEQALQANGTVPLLLATLCGTWMQIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5VT28</accession>
    <name>R5VT28_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATP-dependent Clp protease ATP-binding subunit ClpX</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01428</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000153">
        <property type="protein sequence ID" value="CCZ83692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01428"/>
    <dbReference type="EnsemblBacteria" id="CCZ83692">
        <property type="protein sequence ID" value="CCZ83692"/>
        <property type="gene ID" value="BN566_01428"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ClpB_D2-small" evidence="1">
        <location>
            <begin position="53"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="163" mass="17887" checksum="FCC355D47240FDB0" modified="2013-07-24" version="1">MTPSSVGFGAPISNKKAEMTNIFRQAIPQDLVKFGLVPELVGRLPVITTLDDLDEPALVRILTEPKNALVKQYQKLFGYDHMKLEITPEALTEIAAKAIQRKTGARGLRSIMESILVQTMYDVPSLEQAKKVVVTKECVTEGKQPEILDAEGKPIAVQAESAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5VT73</accession>
    <name>R5VT73_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AMP-binding enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01477</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000160">
        <property type="protein sequence ID" value="CCZ83742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01477"/>
    <dbReference type="EnsemblBacteria" id="CCZ83742">
        <property type="protein sequence ID" value="CCZ83742"/>
        <property type="gene ID" value="BN566_01477"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-binding" evidence="1">
        <location>
            <begin position="9"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding_C" evidence="2">
        <location>
            <begin position="450"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="550" mass="62120" checksum="83727C704EF69709" modified="2013-07-24" version="1">MPITELLRQNAERYPDDTSLVEITPPPTVHGRLTWKEYALIESGTGQNVRREITWKDFNCQANRFANFLLSRGIRKGEKVAILLMNCLEWLPIYFGILKAGAIAVPLNYRYTAEEIRYCLNLAEADFLVFGPEFTGRVEEICDCMPRVRSMLYVGKDCPTFAESYDRLTQFCTSKNPDVPLTEDDDAAIYFSSGTTGFPKAILHTHRSLSHAAQVEQHHHGQTKDDVFLCIPPLYHTGAKMHWFGSLLTGGKAVLLRGVKPEWILKAVSEEQCTIVWLLVPWAQDLLDALDRKDLNLDDYNLDQWRLMHIGAQPVPPSLVKRWKAYFPHHAYDTNYGLSESIGPGCVHLGVENPHKVGAIGKAGYGWEVKIVDPNHNTVPQGEVGELAVKGPGVMKCYYRDEKATEEVLQDGWLFTGDMAQEDEDGFIYLVDRKKDVIITGGENIYPVQIEDFLRQHNAIKDVAVIGLPDARLGEITAAIVEVKPEATLTEEELQAFCMALPRYKRPRKIIFADVPRNPTGKIEKPKLRKRYCVENLVAKESGVTEHETP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5VTF1</accession>
    <name>R5VTF1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Amino acid ABC transporter substrate-binding protein PAAT family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01547</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000165">
        <property type="protein sequence ID" value="CCZ83812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01547"/>
    <dbReference type="EnsemblBacteria" id="CCZ83812">
        <property type="protein sequence ID" value="CCZ83812"/>
        <property type="gene ID" value="BN566_01547"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001638">
        <property type="entry name" value="Solute-binding_3/MltF_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00497">
        <property type="entry name" value="SBP_bac_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00062">
        <property type="entry name" value="PBPb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004407313" evidence="2">
        <location>
            <begin position="31"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="PBPb" evidence="3">
        <location>
            <begin position="34"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="258" mass="28169" checksum="20CB3C6B75FAC8C8" modified="2013-07-24" version="1">MKKFTKVVSLMLAAILMMALCTACGGDSKKDDSTITFGTNAEFPPFEYVTAKGVIGEFDGIDMAIAKQIGEDNGMEAKIENMEFDSLLLALENGQVDAVIAGMTITDEKKDAVDFSKPYYTAKQVMIVKEDSDIQKASDMADKKIVVLQGFTGETCVNEMGYKYESFKKGTEAIMELVNGKCDVVVIDSATAEKYVEDNEGLKIVEDNDAFEAEEYGIAVKKGNTELLDKINKSLDKMMEDGTISELSAKYAESSDAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>R5VTH2</accession>
    <name>R5VTH2_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphoenolpyruvate carboxykinase [GTP]</fullName>
            <shortName evidence="8">PEP carboxykinase</shortName>
            <shortName evidence="8">PEPCK</shortName>
            <ecNumber evidence="8">4.1.1.32</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">GTP-dependent phosphoenolpyruvate carboxykinase</fullName>
            <shortName evidence="8">GTP-PEPCK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">pckG</name>
        <name evidence="11" type="ORF">BN566_01567</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>GTP + oxaloacetate = CO2 + GDP + phosphoenolpyruvate</text>
            <dbReference type="Rhea" id="RHEA:10388"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="4.1.1.32"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="8">Binds 1 Mn(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.32" evidence="8"/>
    <dbReference type="EMBL" id="CBAR010000165">
        <property type="protein sequence ID" value="CCZ83832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01567"/>
    <dbReference type="EnsemblBacteria" id="CCZ83832">
        <property type="protein sequence ID" value="CCZ83832"/>
        <property type="gene ID" value="BN566_01567"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004613">
        <property type="term" value="F:phosphoenolpyruvate carboxykinase (GTP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00819">
        <property type="entry name" value="PEPCK_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.449.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00452">
        <property type="entry name" value="PEPCK_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018091">
        <property type="entry name" value="PEP_carboxykin_GTP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013035">
        <property type="entry name" value="PEP_carboxykinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008209">
        <property type="entry name" value="PEP_carboxykinase_GTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035077">
        <property type="entry name" value="PEP_carboxykinase_GTP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035078">
        <property type="entry name" value="PEP_carboxykinase_GTP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008210">
        <property type="entry name" value="PEP_carboxykinase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11561">
        <property type="entry name" value="PTHR11561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00821">
        <property type="entry name" value="PEPCK_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17297">
        <property type="entry name" value="PEPCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001348">
        <property type="entry name" value="PEP_carboxykinase_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68923">
        <property type="entry name" value="SSF68923"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00505">
        <property type="entry name" value="PEPCK_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 8" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="8" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="5 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 8" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PEPCK_N" evidence="10">
        <location>
            <begin position="12"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="PEPCK_GTP" evidence="9">
        <location>
            <begin position="230"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="205"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="387"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="258"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="257"/>
            <end position="262"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="389"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="420"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="514"/>
            <end position="517"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ83832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="612" mass="67778" checksum="BE4F8EF342134ABE" modified="2013-07-24" version="1">MALTNNKNVLAWVDEMVALTKPDKVVWIDGSKEQLDALTEEVTSLPEGNRDKMYKLNPEKLPGCLYHRTLPNDVARVEDRTFICTKKKEDAGPTNNWMAPDEMKAKLVPLYDGVMKGRTMYVIPYSMGPIGSPLAKVGVELTDSIYVVLNMNIMTRMGKQAFENLGDVSNDFVRGLHSKADVDPENRYIVQFPEENTIWSINSAYGGNVLLGKKCFALRIASFQGKNEGWMAEHMLILGVQKPSGETVYITAAFPSACGKTNLAMLIPPEGYQKAGYKVFTVGDDIAWMKPGKDGRLYAINPENGFFGVAPGTNEKSNPNALACTRKNTIFTNVALNNADNTVWWEKLDKNPPVDATEWKGSKVNGPEFVAEVDEKTGKNKTLAHPNSRFTAPAENCPCISPEFNNPAGVPISAIIFGGRRASTTPLVYQSFDWTHGTYMGSAVSSETTAAATGAVGVLRHDPMAMKPFIGYNVGDYWAHWLEMGKILGDKAPKIFNVNWFKQDEEGNFLWPGFGDNMRVLDWIIKRCEGTVDAVETAIGYLPKKEDLNLTGIEDEVTPAVLDKLLAVDTDLWKKEIAEMRRYYTEDIAAKGGKVPAELMAELDKIEARLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5VTN1</accession>
    <name>R5VTN1_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Cell division protein FtsX</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN566_01611</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Part of the ABC transporter FtsEX involved in asymmetric cellular division facilitating the initiation of sporulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ABC-4 integral membrane protein family. FtsX subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000169">
        <property type="protein sequence ID" value="CCZ83882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01611"/>
    <dbReference type="EnsemblBacteria" id="CCZ83882">
        <property type="protein sequence ID" value="CCZ83882"/>
        <property type="gene ID" value="BN566_01611"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004513">
        <property type="entry name" value="ABC_transpt_FtsX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040690">
        <property type="entry name" value="FtsX_ECD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47755">
        <property type="entry name" value="PTHR47755"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18075">
        <property type="entry name" value="FtsX_ECD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003097">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8 11" id="KW-0132">Cell division</keyword>
    <keyword evidence="4 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="25"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="168"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="220"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="263"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX_ECD" evidence="10">
        <location>
            <begin position="60"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR003097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF18075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ83882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="301" mass="33327" checksum="C8AA9168B4D3DC92" modified="2013-07-24" version="1">MKVSGLKYLAKQGVENIWKNRMMSFASFCVLLVSLLLVGVATLFYMNISSMMGGIENKNEIIVYLDENTTQEEVSVLKGQLSQIDNIAEVNFYSKEESFEDLKNSMDGYEMLFDSLGDDNPLVDAYRLRVKNIDQIADTIVQIESLDHIYSIRAPMDFVNLLMELRRIITIVCSVIIVALIVISLVIIANTTKASVFARRSEIQIMKYVGATNAFIRIPFFVEGMVTGFLAGCVAFVITWVSYDSLINLVTNQTDFVSVIGRGSIMSFGQVAGVVALAYIGSGVLFGALGSVLSMRKHLNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5VTX6</accession>
    <name>R5VTX6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">HAD superfamily hydrolases YKRA B.subtilis ortholog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01701</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000177">
        <property type="protein sequence ID" value="CCZ83972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01701"/>
    <dbReference type="EnsemblBacteria" id="CCZ83972">
        <property type="protein sequence ID" value="CCZ83972"/>
        <property type="gene ID" value="BN566_01701"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000150">
        <property type="entry name" value="Cof"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00099">
        <property type="entry name" value="Cof-subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="263" mass="29623" checksum="8A130C946A04F39E" modified="2013-07-24" version="1">MKKLLFFDIDGTLVDDDGSIPASTIQAIASAQKAGHLTFINTGRTWGNVDPELRAIGFDGFICGCGTEIIYRNQVLFHQQVKPELCASIRELVRSCNATPLYERSDTMFYDPTMPKHPAFCDLMKIYERKQKDVQDLTDHADFGFDKFVIWYDEQTDLKKFQDGIAADFDFIDRENGFAEMVPKNCSKATGMQQLVDYLQMDMKDTIAFGDSLNDLPMMQAANIAVGMGNGTSLHPYVTFITKDIKENGIYEAMKQLNLLSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>R5VUB6</accession>
    <name>R5VUB6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ATPase P-type (Transporting) HAD superfamily subfamily IC/heavy metal translocating P-type ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_01833</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000187">
        <property type="protein sequence ID" value="CCZ84107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01833"/>
    <dbReference type="EnsemblBacteria" id="CCZ84107">
        <property type="protein sequence ID" value="CCZ84107"/>
        <property type="gene ID" value="BN566_01833"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="SSF81653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-1278">Translocase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ84107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="695" mass="75501" checksum="C84F730780C4915A" modified="2013-07-24" version="1">MNGKIVYDKPGRIRFRCGAYAFERELETAIQEALLRNRFVLQVEVHAQNGGILVHYRNGHRLDVIRLVARMNPRTMQPLENQIGTEQIDSDFKNDLAKLVAKRVLYKALLPTPIGNAMIFLRGLRYVGRAVKNLLDCHLNVEVLDGTSVAACLLQKNYKTAGTIMFLLSVSSLLEDYTRARTKAALTDSLAIKTEQVWVVDGDVERLMPMADLQVDDCIRVRTGSIIPVDGTIVDGEANINESSMTGEPLSVMKSAGATVFAGTVVEEGTIVIQVKAVSGNTKLQKIIELIDHSENLKAGVQSRAEHLADSIVPFSFLGFGLTLLLTRNITKAVSILMVDYSCAIKLSTPISVISAIREAADCDITIKGGKYLEAFAEADTIVFDKTGTLTNAEPVLEKVIPFGAYTESEVLKTAACLEEHFPHSVARAIVKGAAEQNLHHEEEHAEVQYIVAHGIATTLHGERAIIGSKHFVAEDEGIVITPEQQAEIDAKSGACSVVYLAIGSELAGVLCIADPPRAEAKQAITMLRDAGISNLVMLTGDSEQAASRTAEMLGITQYHAQVLPEDKHRYVEELKAEGKRVIMVGDGINDAPALAAANVSVAMSDASDIAREAADITLRGANLTELATLRKLSERLMKRIHSNYRFILGFNSTLLLLGLLSVLPPATSAFLHNASTMAICAKSMTPLLKQEDRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>R5VUD2</accession>
    <name>R5VUD2_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NADPH-dependent 7-cyano-7-deazaguanine reductase</fullName>
            <ecNumber evidence="1">1.7.1.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">7-cyano-7-carbaguanine reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">NADPH-dependent nitrile oxidoreductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">PreQ(0) reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">queF</name>
        <name evidence="2" type="ORF">BN566_01848</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>7-aminomethyl-7-carbaguanine + 2 NADP(+) = 7-cyano-7-deazaguanine + 3 H(+) + 2 NADPH</text>
            <dbReference type="Rhea" id="RHEA:13409"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:45075"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58703"/>
            <dbReference type="EC" id="1.7.1.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">tRNA modification; tRNA-queuosine biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.7.1.13" evidence="1"/>
    <dbReference type="EMBL" id="CBAR010000187">
        <property type="protein sequence ID" value="CCZ84122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01848"/>
    <dbReference type="EnsemblBacteria" id="CCZ84122">
        <property type="protein sequence ID" value="CCZ84122"/>
        <property type="gene ID" value="BN566_01848"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00392"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046857">
        <property type="term" value="F:oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033739">
        <property type="term" value="F:preQ1 synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008616">
        <property type="term" value="P:queuosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00818">
        <property type="entry name" value="QueF_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043133">
        <property type="entry name" value="GTP-CH-I_C/QueF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029500">
        <property type="entry name" value="QueF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016856">
        <property type="entry name" value="QueF_type1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14489">
        <property type="entry name" value="QueF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03139">
        <property type="entry name" value="QueF-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="1" id="KW-0671">Queuosine biosynthesis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="1">
        <location>
            <begin position="78"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="1">
        <location>
            <begin position="97"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="active site" description="Thioimide intermediate" evidence="1">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="165" mass="19381" checksum="AE04AC6050AEF6F7" modified="2013-07-24" version="1">MTGRTKEETNGVTLLGNQHTQYPNDYDPKVLETFVNKHPDHDYFVKFNCPEFTSLCPITGQPDFATLYISYVPDEIMVESKSLKLYLFSFRNHGDFHEDCVNIIMNDLIELMHPRYIEVWGKFLPRGGISIDPYCNYGMPGTKWEQVAWERLTHHDLYPETVRNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5VUS3</accession>
    <name>R5VUS3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative arsenical pump membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00201</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000204">
        <property type="protein sequence ID" value="CCZ84272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84272">
        <property type="protein sequence ID" value="CCZ84272"/>
        <property type="gene ID" value="BN566_00201"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019660">
        <property type="entry name" value="Put_sensory_transdc_reg_YbjN"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10722">
        <property type="entry name" value="YbjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="160" mass="18288" checksum="EE7FB59A3214B07B" modified="2013-07-24" version="1">MAKNYSPEIAKAVEEFFTKEELNYIPCNENGVIRSGMMLSSQIHRVDIYMDIRKNGYSMISVFPLHAEEKARPAIAEFLTRANYEINLGCFDMDFEDGEIRYKMAQFCGATVPTYEQIQKIFYVAITTINHYGNGIAKTLLSLGTPKENLAECHQEPKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVP3</accession>
    <name>R5VVP3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00443</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000247">
        <property type="protein sequence ID" value="CCZ84582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00443"/>
    <dbReference type="EnsemblBacteria" id="CCZ84582">
        <property type="protein sequence ID" value="CCZ84582"/>
        <property type="gene ID" value="BN566_00443"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="190"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="214"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="247"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="286"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="319"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="358"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="384" mass="43393" checksum="07B52A6A47130C7C" modified="2013-07-24" version="1">MQKTIFRTKSTIPCRDDAELASEQTYQTAFTAWQEQPGNEFSFRQPGSTTQAVFLEGMGLQEEEPAVLERRALQRISNQLGILFLHQLLSPYLVLIFCVVVFSLLGGSLSYSVHDNVLYGSNDLVLITMILRKIVRFGVPIFMAKRLLKMPKAVTCCRQKTVPSTWIRTLGITMCVFFAFGMILNFLPESSIHYITLGALSHTILCMDTQHQIFYFIFVLIFRPIFETMLYNGSIMHVLRQFGDDTAVLLTATFSALMAENLVTGISTFAVSILAAREILRDENIYVGLFSRSVYYTLMFVLFHTSIFRNNVPIPNRMLWILIIGIVGMLLGTLVIWKAPFQGSKRFYSIRSAGKCSLIFLIQANLMTVCVFVSLALSVVSLIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVQ9</accession>
    <name>R5VVQ9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative peptidoglycan-binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00096</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000252">
        <property type="protein sequence ID" value="CCZ84602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00096"/>
    <dbReference type="EnsemblBacteria" id="CCZ84602">
        <property type="protein sequence ID" value="CCZ84602"/>
        <property type="gene ID" value="BN566_00096"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.101.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002477">
        <property type="entry name" value="Peptidoglycan-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036366">
        <property type="entry name" value="PGBDSf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01471">
        <property type="entry name" value="PG_binding_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="SSF47090"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PG_binding_1" evidence="1">
        <location>
            <begin position="9"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="PG_binding_1" evidence="1">
        <location>
            <begin position="103"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="169" mass="19105" checksum="A986B7F89165D8DE" modified="2013-07-24" version="1">MAYTEQDRRNHIRELQQYLYSLSFLDETLPRVIPDGIYGRQTALAVRAFQQKNGLRPNGETNRATWDAIVNAYRQQIQREATPLHLYTPERELLGAGETGLLVLVLQSILKTLGVRYTNIQNLQITGIYDAPTQKAVQNFQSLTARKPTGMVDRATWNLLVAAAECSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVW7</accession>
    <name>R5VVW7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA-binding protein KhpA</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">KH-domain protein A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">khpA</name>
        <name evidence="3" type="ORF">BN566_00525</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">A probable RNA chaperone. Forms a complex with KhpB which binds to cellular RNA and controls its expression. Plays a role in peptidoglycan (PG) homeostasis and cell length regulation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Forms a complex with KhpB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the KhpA RNA-binding protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000267">
        <property type="protein sequence ID" value="CCZ84672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00525"/>
    <dbReference type="EnsemblBacteria" id="CCZ84672">
        <property type="protein sequence ID" value="CCZ84672"/>
        <property type="gene ID" value="BN566_00525"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00088">
        <property type="entry name" value="UPF0109"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020627">
        <property type="entry name" value="KhpA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34654">
        <property type="entry name" value="PTHR34654"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="SSF54814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0133">Cell shape</keyword>
    <keyword evidence="2" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="77" mass="8307" checksum="C3F8360A6F37148D" modified="2013-07-24" version="1">MQELLRTIVAGLVEDPSAIAITEDAPNEEGVIVYHLHVAADDMGRVIGKQGRIARAIRIIMRSGAAKSNQKIAVEID</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVY7</accession>
    <name>R5VVY7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Stage V sporulation protein AC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00550</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000270">
        <property type="protein sequence ID" value="CCZ84697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00550"/>
    <dbReference type="EnsemblBacteria" id="CCZ84697">
        <property type="protein sequence ID" value="CCZ84697"/>
        <property type="gene ID" value="BN566_00550"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005562">
        <property type="entry name" value="SpoVA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38450">
        <property type="entry name" value="PTHR38450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03862">
        <property type="entry name" value="SpoVAC_SpoVAEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="149" mass="15876" checksum="14F554C7BFBF7F18" modified="2013-07-24" version="1">MKVSRELYQEMQNQTAPKTKSYYTIPMAFLIGGFICTLGELLLHLYAFLGADQTAAAAFTSITLIGCSVLFTGLGWYEKIAKHAGAGTLVPITGFANAVSCCAIESRAEGWILGVGAKIFTIAGPVIVYGTAASMLYGLIYYLATLGNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5UWX6</accession>
    <name>R5UWX6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00628</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000011">
        <property type="protein sequence ID" value="CCZ82823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82823">
        <property type="protein sequence ID" value="CCZ82823"/>
        <property type="gene ID" value="BN566_00628"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004406507" evidence="1">
        <location>
            <begin position="31"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="318" mass="35369" checksum="1680CC8A973E9228" modified="2013-07-24" version="1">MKKSAIKMIAGVSALAIFSSISATAIPAFAEEYDAIQEQDYVATFADEQTQNVASYLLENGVSMEEAQEMMEHYVNGRAMIEQRENAENGISLLSDDEEEPKVFYNGENFTSYKNSIKSSKPSTTFKKEQHYGAFIINDPIANVRVRFNAISNEDANGNYLITGTGDYYIFNNYNNFTSFGLDSDGVSFKTETKTNSAGRPLIMCTFSFDASDSLKSEAAVKNAIQVQKTASLLKNGNQINSDFTFHTFVLGDFDHDGVVCDDDYAYLMDFVMNSFDGYISYKDVSDKMAFEVNTTAVDLNMDGVLDIRDAIAWNKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>R5UX66</accession>
    <name>R5UX66_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Iron-sulfur cluster carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00700</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Binds and transfers iron-sulfur (Fe-S) clusters to target apoproteins. Can hydrolyze ATP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Mrp/NBP35 ATP-binding proteins family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000022">
        <property type="protein sequence ID" value="CCZ82898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00700"/>
    <dbReference type="EnsemblBacteria" id="CCZ82898">
        <property type="protein sequence ID" value="CCZ82898"/>
        <property type="gene ID" value="BN566_00700"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140663">
        <property type="term" value="F:ATP-dependent FeS chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02040">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019591">
        <property type="entry name" value="Mrp/NBP35_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044304">
        <property type="entry name" value="NUBPL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033756">
        <property type="entry name" value="YlxH/NBP35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42961">
        <property type="entry name" value="PTHR42961"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10609">
        <property type="entry name" value="ParA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="45"/>
            <end position="52"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ82898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="278" mass="29850" checksum="032CBEC7887F2C89" modified="2013-07-24" version="1">MGEACNHQCDSCGSNGNCSTQDLEHEKRVKEHTSGVKNVIAVVGGKGGVGKSLVTSLLAIQTQRLGYHAAILDADITGPSIPKIFGINKPAEAVDNSLLPHVTESGVKIMSMNMLLPNATDPVLWRGPVISGVVKQFWEDVQWGDVDFLFVDMPPGTGDVPLTVFQSLPINGILIVSTPQSLVSMIVDKAMNMAQMMQVPVLGMVENMRFLRCPDCGKEIPLFGSDDHVDSHAVPVLERIPLDPTVAAACDAGKLEQAEIDYLAKTAVMLTETFGKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXP7</accession>
    <name>R5UXP7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00861</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000051">
        <property type="protein sequence ID" value="CCZ83073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00861"/>
    <dbReference type="EnsemblBacteria" id="CCZ83073">
        <property type="protein sequence ID" value="CCZ83073"/>
        <property type="gene ID" value="BN566_00861"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="336"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="355" mass="40422" checksum="B02C4C03120DB785" modified="2013-07-24" version="1">MAKTIDEIMAEARNGLTGEKSVDMVHLEHIAEEYAGTEIGKEVESQIADLAYEILPDEQKERVAKMLYIDGKRMDKVFAEAVELVNQKKIKEAFKLTEALYTKICINFRETEEHLYLSMRNTLEHQLYLFFYHPSKKLIRAPFDLCAMVMLHGYVLLDLGRAEEAVSVLEDAIRYNPLNSDPYFEMAECYKYLKQPEDLLGVTKETLSVATTPVTLSRCYCNLGFYCIEKKDYDSAICFYYESLIYADHPGVQAELHHIHTITQKKIVPPTREEVNKAFEKYGMEPGASKEVVGVAAALAKEAIEKKDLSGSQFYLLVVYSLTNDPEVKELLEKVSPKKKAAKTEAGEKPIQQTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXQ1</accession>
    <name>R5UXQ1_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Elongation factor 4</fullName>
            <shortName evidence="6">EF-4</shortName>
            <ecNumber evidence="6">3.6.5.n1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Ribosomal back-translocase LepA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">lepA</name>
        <name evidence="8" type="ORF">BN566_00866</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>GTP + H2O = GDP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.n1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
            <orientation evidence="6">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. LepA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.n1" evidence="6"/>
    <dbReference type="EMBL" id="CBAR010000051">
        <property type="protein sequence ID" value="CCZ83078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00866"/>
    <dbReference type="EnsemblBacteria" id="CCZ83078">
        <property type="protein sequence ID" value="CCZ83078"/>
        <property type="gene ID" value="BN566_00866"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045727">
        <property type="term" value="P:positive regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03709">
        <property type="entry name" value="lepA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00071">
        <property type="entry name" value="LepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006297">
        <property type="entry name" value="EF-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038363">
        <property type="entry name" value="LepA_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013842">
        <property type="entry name" value="LepA_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035654">
        <property type="entry name" value="LepA_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43512">
        <property type="entry name" value="PTHR43512"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06421">
        <property type="entry name" value="LepA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="SSF54980"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01393">
        <property type="entry name" value="lepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="4 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="7">
        <location>
            <begin position="2"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="14"/>
            <end position="19"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="131"/>
            <end position="134"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="599" mass="66795" checksum="B5C2431AFFA0F2D6" modified="2013-07-24" version="1">MEQIRNFCIIAHIDHGKSTLADRILEQTFTVAKRDMEEQILDNMDIERERGITIKARAVRLNYKAKDGKTYVFNLIDTPGHVDFNYEVSRSLAACEGAILVVDASQGIEAQTLANTYLAIEHDLEVVPVVNKIDLPAADPERVCQEIESVIGIPAMDAPRISAKEGINIGEVMERIVTDIPAPNGDPNAPLKALIFDSYYDAYKGVVIYIRVKEGTVKVGDTIRLMATGAEFTIVEAGYMDTKRLVNVGQLSAGEVGYLSASIKSIGDTQVGDTVTLADNPCAEPLPGYRKVNPMVFSGIYPADGAKYGDLREALEKLQLNDASLSFEPETSIALGFGFRCGFLGLLHMEIIQERLEREYNLDLITTAPSVIYRVTMTNGDVEMIDNPTNYPDPALIQLAEEPMVKAEILTPPDYVGNVMELCQNRRGVFLDMQYLDDTRVSLKYHLPLNEIVYDFFDVLKSRTKGYASFDYEVTGYAASQLVKLDILLNGDVIDALSFIIHKDKAYERARKIVEKLKENIPRQLFEVPVQAAIGGKIIARETIKAMRKDVLAKCYGGDITRKKKLLEKQKEGKKRMRQLGTVEVPQEAFMSVLKLDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXT0</accession>
    <name>R5UXT0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">VanZ family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00687</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000022">
        <property type="protein sequence ID" value="CCZ82885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00687"/>
    <dbReference type="EnsemblBacteria" id="CCZ82885">
        <property type="protein sequence ID" value="CCZ82885"/>
        <property type="gene ID" value="BN566_00687"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016747">
        <property type="entry name" value="Phosphotransbutyrylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006976">
        <property type="entry name" value="VanZ-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04892">
        <property type="entry name" value="VanZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019083">
        <property type="entry name" value="UCP019083_VanZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="VanZ" evidence="2">
        <location>
            <begin position="14"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ82885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="171" mass="19384" checksum="3682ADB9B35C37CC" modified="2013-07-24" version="1">MQTQKFYQRSGFWFCCTLLWCVVIFCFSAQDATQSNAVSNGVLHKIAEICTAWIPADAMDLESSTHTSMAFLIRKIAHFTLYFILGLLSCATMMTYCLKKEHSLSAGWFCAWAFCIFYATTDEFHQLFVPGRTGRLLDIGIDSCGALLGSLILLFFVYRADQRHQKLEKGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXV0</accession>
    <name>R5UXV0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cell division protein FtsI/penicillin-binding protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00935</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000064">
        <property type="protein sequence ID" value="CCZ83148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="SMR" id="R5UXV0"/>
    <dbReference type="STRING" id="1262953.BN566_00935"/>
    <dbReference type="EnsemblBacteria" id="CCZ83148">
        <property type="protein sequence ID" value="CCZ83148"/>
        <property type="gene ID" value="BN566_00935"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008658">
        <property type="term" value="F:penicillin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036138">
        <property type="entry name" value="PBP_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001460">
        <property type="entry name" value="PCN-bd_Tpept"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00905">
        <property type="entry name" value="Transpeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56519">
        <property type="entry name" value="SSF56519"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004406546" evidence="1">
        <location>
            <begin position="22"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="Transpeptidase" evidence="2">
        <location>
            <begin position="219"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="541" mass="57860" checksum="23FC9E118FD606BB" modified="2013-07-24" version="1">MQSIRRRITMLCLTMLMLAAAIAVRLAYLISVPNYQETARQQGTYTIRTPTTYANLYDANFQKLVNDTVQQIAVINPTTEAVTAILPYVQDKEAFYENLQYGTPFSCVVTTNHVDCADIQIFSVPIRTTEPQLAQHLIGYTRDGEGVTGLEADYNALLHSVSSQSSVTFTVNGSGGVLAGEQTLIHTGQSISLGVVTTLHKEVQQICETVGKSCGLKKGCIVVLDAQNGEILALCSFPSYTVSTLGEALESPDSPLINRALYAYPVGSIFKLVPAACALSQNLSQFSYTCTGSISIYDQVFHCHDRNGHGKQDLQAAMIHSCNPYFIALSQKLSAATLFQTAEKLGYGTEIPLTDSISADGGILPTLQDLKISAEKANFCFGQGMLTASPLQVARMTCAIANGGTLPQPRLIRGITKDGISLVTEPETTATPALSPNTATALQELMIAAAYGSDTFQGVPDGITVGAKTSTAQTGRYDENGVEYCHGWITGFFPASKPRYVVTVLAEDGGYGNDAAAPIFREVIEQIVQQKALPLSGAALS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXW6</accession>
    <name>R5UXW6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glycosyl hydrolase family 43</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00715</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the glycosyl hydrolase 43 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000027">
        <property type="protein sequence ID" value="CCZ82915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00715"/>
    <dbReference type="EnsemblBacteria" id="CCZ82915">
        <property type="protein sequence ID" value="CCZ82915"/>
        <property type="gene ID" value="BN566_00715"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.115.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006710">
        <property type="entry name" value="Glyco_hydro_43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04616">
        <property type="entry name" value="Glyco_hydro_43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="SSF75005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="site" description="Important for catalytic activity, responsible for pKa modulation of the active site Glu and correct orientation of both the proton donor and substrate" evidence="2">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR606710-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ82915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="532" mass="60692" checksum="3FE2E0DB38840BAF" modified="2013-07-24" version="1">MQKRQVFNPFLPLNEYIPDGEPHVFGDRVYIYGSHDKEGGYTFCMQDYVTYSAPVNNLSDWRYEGVIYKASQDSRYPNPQYLFAPDVVQGNDGKYYLYYCMGGDYGCKGYTGPIGVAVSDSPAGKFKFLGYVKNPDGTPMMKYVCFDPAAINDNGTIRIYYGTQYGYEEQDDFNENEKYINEEIEMFGRTKEEILSYPDSIMGPSMVVLEDDMLTVKEEAKHIIPYRVKGTSFERHPFFEGSSMRKVGDKYYFIYSSWQNHELCYATSDYPDKDFIFGGTIVSNGDIGYNGRTESEKLNMTGTTHGSIIEINNQWYVFYHRLTHKSDYSRQACAEKIYIDSDGNIAQVEVTSCGLNDEPLKAEGEYPSIIACNITNGHMPHGSNSIYAFPFPNVTNIGDERFIAEVENETLIGYKYFKFSGNKNISVTIRKETENNKVKLEAPKRIDERCDAEVKRCASFKDNAKIAEESYIDILNEEKGKSIGRISIPDNLEDKWCRIGGNVNFPDGVYPLYFVYHGKNSIQIKSFDFIGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5UY13</accession>
    <name>R5UY13_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000074">
        <property type="protein sequence ID" value="CCZ83218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83218">
        <property type="protein sequence ID" value="CCZ83218"/>
        <property type="gene ID" value="BN566_01001"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="185" mass="20873" checksum="253617C9F9F9C234" modified="2013-07-24" version="1">MQTKDMISAYQTLNQVAEAAGVVLFGSTTAANLPLNELLQDFGVSCTVYNRSVEGLTIEQAESYIQPCIAPLMPKMILLHFGEEELNAGTQSVDSIIEAYRWLLYQLHTAMPDSRLVLISVNKQLPQATIYNRALQELAKEYGCQYAEAPTGEMNEAYELQFFQTLRGFFYDHTMNLSDIFRYCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYD5</accession>
    <name>R5UYD5_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nucleoid-associated protein BN566_00823</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00823</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to DNA and alters its conformation. May be involved in regulation of gene expression, nucleoid organization and DNA protection.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Nucleoid</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the YbaB/EbfC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000040">
        <property type="protein sequence ID" value="CCZ83030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00823"/>
    <dbReference type="EnsemblBacteria" id="CCZ83030">
        <property type="protein sequence ID" value="CCZ83030"/>
        <property type="gene ID" value="BN566_00823"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043590">
        <property type="term" value="C:bacterial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1310.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00274">
        <property type="entry name" value="DNA_YbaB_EbfC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036894">
        <property type="entry name" value="YbaB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004401">
        <property type="entry name" value="YbaB/EbfC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33449">
        <property type="entry name" value="PTHR33449"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02575">
        <property type="entry name" value="YbaB_DNA_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004555">
        <property type="entry name" value="UCP004555"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82607">
        <property type="entry name" value="SSF82607"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00103">
        <property type="entry name" value="DNA_YbaB_EbfC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="115" mass="12425" checksum="44A207BB2426378B" modified="2013-07-24" version="1">MKARLPKQNMGGAQNMNAMIRQAQKMQDEITTLQEDIENREFSATSGGGAVSVVVTGKKIIKSLTINKEVVDPEDVEMLQDLVISAINEAVNQVESTTETEMSKITGGVSLPGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYE8</accession>
    <name>R5UYE8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Mg chelatase-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00838</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Mg-chelatase subunits D/I family. ComM subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000040">
        <property type="protein sequence ID" value="CCZ83045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00838"/>
    <dbReference type="EnsemblBacteria" id="CCZ83045">
        <property type="protein sequence ID" value="CCZ83045"/>
        <property type="gene ID" value="BN566_00838"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032508">
        <property type="term" value="P:DNA duplex unwinding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045006">
        <property type="entry name" value="CHLI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004482">
        <property type="entry name" value="Mg_chelat-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025158">
        <property type="entry name" value="Mg_chelat-rel_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000523">
        <property type="entry name" value="Mg_chelatse_chII-like_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32039">
        <property type="entry name" value="PTHR32039"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32039:SF7">
        <property type="entry name" value="PTHR32039:SF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01078">
        <property type="entry name" value="Mg_chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13335">
        <property type="entry name" value="Mg_chelatase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00368">
        <property type="entry name" value="TIGR00368"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="2">
        <location>
            <begin position="211"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="510" mass="55678" checksum="57CA51DADB198AD5" modified="2013-07-24" version="1">MFATIKSLGLFGLNAFPVDVEVDIQKGLPHFDIVGLPDAAVKESRERIRAALYSISVTLPPKHIVVNLAPADVKKSGTMHDLAILVGVLKSMGQLSRSMQGKFFIGEVSLSGEVRSVNGVLPMTILARSLGVEELYVPADNAAEAATIQDVRVFGVHNIGELIDHLEGDAPLSPAQPADFTESDAPENLLDFSDVKGQQTVKRALEIVAAGGHNVLLVGAPGSGKSMLAKRMPYILPEMTMEESIETTNIYSISGLLDPKHPLITKRPFRAPHHTISGVGLVGGGTVPHPGEISLAHNGLLFLDELAEFDRHTLDILRQPLEDHIVTITRAAGTISYPCRFMLVGAMNPCPCGYYGHPKRRCSCSPQAIQRYRSRISGPLLERFDLQVEVDPVSYEDLTTTEPIETSADIRKRVQAAREIQEKRFSGTSIFCNAQIPAGKLQQYCPMEETAERLVQDAFDRLGLSARSYDRLLKVGRTIADLAGSACILRRHMAAAIQYCSLSWKYLPPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYJ4</accession>
    <name>R5UYJ4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01164</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000094">
        <property type="protein sequence ID" value="CCZ83388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83388">
        <property type="protein sequence ID" value="CCZ83388"/>
        <property type="gene ID" value="BN566_01164"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005325">
        <property type="entry name" value="DUF308_memb"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03729">
        <property type="entry name" value="DUF308"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="199" mass="21887" checksum="429EF48BF06F52CD" modified="2013-07-24" version="1">MAATKTKKKTDNKKMMQALQFIMPLLLIGLGVLLIRRRDALNQVFMAIGIVIALIGLIYTVVYYATKQYEKKPPYLASGVGLLIIGGIIIIIPFLADTFIPFCIGLFILISGITGLMDAFQLKKFSSDWQIPLAFSVLITLMGVAVLICTFYLSGVVWIIIGIMLIVSGLMRFINAIISANARKKQIPDDVIEGEATEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYM2</accession>
    <name>R5UYM2_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Dihydroorotate dehydrogenase B (NAD(+)), electron transfer subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Dihydroorotate oxidase B, electron transfer subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">pyrK</name>
        <name evidence="9" type="ORF">BN566_00917</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Responsible for channeling the electrons from the oxidation of dihydroorotate from the FMN redox center in the PyrD type B subunit to the ultimate electron acceptor NAD(+).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5 7">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="5 7">Binds 1 [2Fe-2S] cluster per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5 6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="5 6">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (NAD(+) route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Heterotetramer of 2 PyrK and 2 PyrD type B subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the PyrK family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000062">
        <property type="protein sequence ID" value="CCZ83130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00917"/>
    <dbReference type="EnsemblBacteria" id="CCZ83130">
        <property type="protein sequence ID" value="CCZ83130"/>
        <property type="gene ID" value="BN566_00917"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00945"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.240.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01211">
        <property type="entry name" value="DHODB_Fe_S_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012165">
        <property type="entry name" value="Cyt_c3_hydrogenase_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037117">
        <property type="entry name" value="Dihydroorotate_DH_ele_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019480">
        <property type="entry name" value="Dihydroorotate_DH_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023455">
        <property type="entry name" value="Dihydroorotate_DHASE_ETsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10418">
        <property type="entry name" value="DHODB_Fe-S_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006816">
        <property type="entry name" value="Cyc3_hyd_g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="SSF52343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="SSF63380"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="4 5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5 6" id="KW-0274">FAD</keyword>
    <keyword evidence="5 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5 7" id="KW-0408">Iron</keyword>
    <keyword evidence="5 7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 5" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="8">
        <location>
            <begin position="3"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5 6">
        <location>
            <begin position="51"/>
            <end position="54"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 6">
        <location>
            <begin position="75"/>
            <end position="76"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 7">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 7">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 7">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 7">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR006816-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR006816-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ83130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="257" mass="27564" checksum="0B2F75D4ACBDBB41" modified="2013-07-24" version="1">MHYLQGRYPVLEKKILAKNMVQYVILCPEIAAAAQPGQFVHILPVGHTLRRPISLCGIDKEKGTICIVFELKGSGTETLAACNVGDCMDVLGPLGHGFTLLPDAKRVVLLGGGIGNPPMLPLAQYYQERATVISGFRSAEFVILQEAFQAAGAETILCTDNGTVGRHGLVTEPLQELLQTQQIDAVYACGPKPMLRAIANLANTADVFCEVSLEERMGCGIGACLVCACKLEREGKPVSAHVCKDGPVFSAKEVVWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYV5</accession>
    <name>R5UYV5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00120</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000079">
        <property type="protein sequence ID" value="CCZ83225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83225">
        <property type="protein sequence ID" value="CCZ83225"/>
        <property type="gene ID" value="BN566_00120"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="44"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="100"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="124"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="299" mass="34015" checksum="82AEB82FAA4DE3AE" modified="2013-07-24" version="1">MERLHVSVSRRSLFPLLMALFGALLCIGMVQRFRDPLYDMTLEYWLYLAAGCFMVLVGLVGMRFFPWMCIIPTVCYVYGGVIEGWSDLQANGSSGLLTQIPFYQILMLVPLILLLMIRWKPWCIWLLKGLEIIAILIPFAMAWMQLQDALLELKMEGYLTDAVKQTRWMHFLMNEAVFYAGMICMTLSLRYLPPQEPVEDTEADKDAEIESKAFAPETIPDAEPLEEAPIPQPMPEEPFPQQQPAPEFYGQPEPQATAAPEIAPIPPMQHRGGAYPYNRPIHLPSESHSEEDSQDDSEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYW9</accession>
    <name>R5UYW9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ammonium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN566_01021</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ammonia transporter channel (TC 1.A.11.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000081">
        <property type="protein sequence ID" value="CCZ83240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01021"/>
    <dbReference type="EnsemblBacteria" id="CCZ83240">
        <property type="protein sequence ID" value="CCZ83240"/>
        <property type="gene ID" value="BN566_01021"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001905">
        <property type="entry name" value="Ammonium_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018047">
        <property type="entry name" value="Ammonium_transpt_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43029">
        <property type="entry name" value="PTHR43029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00836">
        <property type="entry name" value="amt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01219">
        <property type="entry name" value="AMMONIUM_TRANSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0924">Ammonia transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="126"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="227"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="258"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="281"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="314"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="353"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium_transp" evidence="9">
        <location>
            <begin position="8"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ83240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="415" mass="44194" checksum="72F085A2343CE181" modified="2013-07-24" version="1">MDSGSIGFVMICAAFVFFMTPGLSYFYGGLVRRKNAVNTMFASVITIGTGIVMWVLFGYSLAFGTDHFGIIGDFSWLGLEGVSKTEPYVEGQQIPNMVFCLFQMMFAVITPALITGAIAGRMRFKSLFFFIVFWSIIVYYPMAHMVWGDGGFLAKIGSVDFAGGNVVHISSGVSALLLCILLGKRYDYARTNYRIHNVPFVAMGAFILLFGWFGFNAGSALAADGLAAHAFVTTAVSSAAGMLSWMLCDVIADKKPTFVGASTGLVAGLVGITPGAGFVPIWAAIIIGFTTSPICFFMISFVKKKLGYDDALDAFGCHGIGGIWGGICTGLFTQASINDIGQGNGLFFGDTKLFLMQLASIGITIVVAVVGTLICYALTKLVSGGNIRVDARTERAGLDRAEHGEAAYPSFTGLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZ36</accession>
    <name>R5UZ36_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pseudouridine synthase</fullName>
            <ecNumber evidence="3">5.4.99.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_01341</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Responsible for synthesis of pseudouridine from uracil.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a uridine in RNA = a pseudouridine in RNA</text>
            <dbReference type="Rhea" id="RHEA:48348"/>
            <dbReference type="Rhea" id="RHEA-COMP:12068"/>
            <dbReference type="Rhea" id="RHEA-COMP:12069"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the pseudouridine synthase RluA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.-" evidence="3"/>
    <dbReference type="EMBL" id="CBAR010000131">
        <property type="protein sequence ID" value="CCZ83573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01341"/>
    <dbReference type="EnsemblBacteria" id="CCZ83573">
        <property type="protein sequence ID" value="CCZ83573"/>
        <property type="gene ID" value="BN566_01341"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120159">
        <property type="term" value="F:rRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd02869">
        <property type="entry name" value="PseudoU_synth_RluA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006225">
        <property type="entry name" value="PsdUridine_synth_RluC/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006224">
        <property type="entry name" value="PsdUridine_synth_RluC/D_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00005">
        <property type="entry name" value="rluA_subfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01129">
        <property type="entry name" value="PSI_RLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S4" evidence="4">
        <location>
            <begin position="14"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR606225-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="311" mass="34443" checksum="95959F567405FC51" modified="2013-07-24" version="1">MKEPLTFTPEAVGQRLDKWLAEQDTLDLTRSVIQTRMEQGDVTVNGAAVSKNYKIRSGDVVQVVIPEPVSLELVPEPIPLDIVYEDDDLLVVNKPKGMVVHPAPGNLTGTLVHALLYHCGDRLSGINGVIRPGIVHRIDKNTSGLLMVAKNDHAHLKLSEQIQAHTFTRAYQAIAIGRFSEKSGTIHAPIGRHPVDRKKMCVTQKNSKDAITHYTVLAESGNYTHLQLQLETGRTHQIRVHLAYLGHPVLGDDVYGKPFPNLTGQCLHAGKIGFMHPTTGEYMEFTSPLPDYFQAALRKLRFTEAEGMVQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZ42</accession>
    <name>R5UZ42_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01351</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000132">
        <property type="protein sequence ID" value="CCZ83583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83583">
        <property type="protein sequence ID" value="CCZ83583"/>
        <property type="gene ID" value="BN566_01351"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="94" mass="10830" checksum="01594AA877895C75" modified="2013-07-24" version="1">MQTIFTAAELKENYQILLRLLGQLQTENDYLQAAMDNLSKIPNNAIPGDIANQAKAIAISELSKNREKTNQKLLETYQKLYEDLRPFLFQENEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZ49</accession>
    <name>R5UZ49_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01356</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000144">
        <property type="protein sequence ID" value="CCZ83593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83593">
        <property type="protein sequence ID" value="CCZ83593"/>
        <property type="gene ID" value="BN566_01356"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="90" mass="9075" checksum="8F5851312782371E" modified="2013-07-24" version="1">MVVVAVVALVVVVVVVSVVVVVVVIAVVCSEVVVVVVGCMVVVIASVVGSVVTDSVISVETVVVVVSTGMDSVAVVVVVVVSGCWFFWSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZB3</accession>
    <name>R5UZB3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HTH tetR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01151</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000093">
        <property type="protein sequence ID" value="CCZ83375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01151"/>
    <dbReference type="EnsemblBacteria" id="CCZ83375">
        <property type="protein sequence ID" value="CCZ83375"/>
        <property type="gene ID" value="BN566_01151"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="6"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="210" mass="23718" checksum="13B14689F2059E6E" modified="2013-07-24" version="1">MLTTETGTQTRILAAAKTEFLEKGFRNASLRTIVKLAGVTTGAFYGYYSNKEALFSALVEPHAAAIMGKFMQAQDHFAQLPDEDQIQQMGMESGACFDWMIDYLYSDQTHYDAVKLIICCSEGTPYQDFIHQMVEVEVDATFQFLKTLHRLGKAVPPIDRQLCHIVSSGMFSGVFEIVVHDMTKENAKSYISALRRFYQAGWAELMQIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZI3</accession>
    <name>R5UZI3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cellobiose 2-epimerase</fullName>
            <shortName evidence="3">CE</shortName>
            <ecNumber evidence="3">5.1.3.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01199</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the reversible epimerization of cellobiose to 4-O-beta-D-glucopyranosyl-D-mannose (Glc-Man).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 3">
            <text>D-cellobiose = beta-D-glucosyl-(1-&gt;4)-D-mannopyranose</text>
            <dbReference type="Rhea" id="RHEA:23384"/>
            <dbReference type="ChEBI" id="CHEBI:17057"/>
            <dbReference type="ChEBI" id="CHEBI:47931"/>
            <dbReference type="EC" id="5.1.3.11"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cellobiose 2-epimerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.11" evidence="3"/>
    <dbReference type="EMBL" id="CBAR010000102">
        <property type="protein sequence ID" value="CCZ83425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01199"/>
    <dbReference type="EnsemblBacteria" id="CCZ83425">
        <property type="protein sequence ID" value="CCZ83425"/>
        <property type="gene ID" value="BN566_01199"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047736">
        <property type="term" value="F:cellobiose epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00929">
        <property type="entry name" value="Cellobiose_2_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028584">
        <property type="entry name" value="Cellobiose_2_epim"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="SSF48208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="390" mass="45526" checksum="3070AF1B8AE904DC" modified="2013-07-24" version="1">MNILLEKARRMLEENIIPFWQGLRDDEYGGYIGHVDFSMHSDCKAEKGCILHSRILWFFSEAALLLNRMDLAADAEHVYSFFTQHCMDREQGGVYWSVSYDGTPMDTTKHTYNQAFALYALSSYYRLTKNPNAKQLAYQLFDLIQEKAFDGAGYLEAFDRNWKPASNEKLSENGVMASRTMNTLLHVFEAYSGFYRATKDKRVAEAMRTILQIYQEKVFNPERNRQEVFFDLDYNSLIDLHSYGHDIESSWLIDDGCSLLGDAALNQTIFAIDDRLAEGIYQTAYRNHSVLNECENGVDDETRVWWVQAESVLGFYHMYEKHGNCQYLQAAQDIFFYIEQYFLVSDHKSEWFWAVDKDGKPMQNLDIVNGWKCPYHNGRMCLELIRRNGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5V023</accession>
    <name>R5V023_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01353</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000134">
        <property type="protein sequence ID" value="CCZ83585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83585">
        <property type="protein sequence ID" value="CCZ83585"/>
        <property type="gene ID" value="BN566_01353"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="445" mass="52755" checksum="D5AFF56430C7E463" modified="2013-07-24" version="1">MERTNTKDIVSKVAYLIGVKKTVLYNQFETERPGYLKELDENQNARTIRSLCKLRMTLLHRFKKIDDMLRFELKNLKTIEFFDKDDIKQLEKWEIPVILVNSRSSLYMEHFNDLIQEHIDACKDLFPEWLKWEYLRSLFLMPKNKKTDVPKSEFSKYMANMEFYPYQTYIYWQHPKDCGNMLFNDNKFVNILYEMHNDYFFETEKVLDAGETTKTTIYDFIERGERTAIIVDCENSDVYKLYATLKNLNQEELQKVKKIILFDDVHTTRAWSCLSHFLDIEVEHIEVERVNDYKSLVDIKMTAGACKEFYSNDIQSFILVSSDSDYWGLISSLPDADFLVMIEYSKCGKDIKEKLENNGIFYCSIDDFCSGNIAELKSYALQEELKGQLEQALNLNAHTLLETVYRNCRIQASETEQKNFYDRYLRTLQLTMDADGNYLIKPKTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5V028</accession>
    <name>R5V028_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Heterodisulfide reductase subunit B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01677</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000175">
        <property type="protein sequence ID" value="CCZ83948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01677"/>
    <dbReference type="EnsemblBacteria" id="CCZ83948">
        <property type="protein sequence ID" value="CCZ83948"/>
        <property type="gene ID" value="BN566_01677"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004017">
        <property type="entry name" value="Cys_rich_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02754">
        <property type="entry name" value="CCG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCG" evidence="1">
        <location>
            <begin position="4"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="CCG" evidence="1">
        <location>
            <begin position="147"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="259" mass="28663" checksum="DE74B9DFF9F79406" modified="2013-07-24" version="1">MTFSYFPGCTLKTKAKDLDRYARASAEALGFTLEELPDWQCCGGVYPMAKNEIATKLSSVRALVSAREKDQALVTLCSACHNVIKQVNHDMQTDENMQMKANNYMKLETPYTGETEVLHYLEVLRDKIGFDKVAAAVKNPLKGKKIAAYYGCLLLRPGKVLQMDNPENPKILEDFIRAIGAEPVLYPQRNECCGGYMTMEEKSVAVSRSSAVMQSAAEFGAEMVITACPLCLYNLKKNSGSELPVVYFTELLAEALGVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0L7</accession>
    <name>R5V0L7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_01545</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000165">
        <property type="protein sequence ID" value="CCZ83810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01545"/>
    <dbReference type="EnsemblBacteria" id="CCZ83810">
        <property type="protein sequence ID" value="CCZ83810"/>
        <property type="gene ID" value="BN566_01545"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010065">
        <property type="entry name" value="AA_ABC_transptr_permease_3TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043429">
        <property type="entry name" value="ArtM/GltK/GlnP/TcyL/YhdX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30614">
        <property type="entry name" value="PTHR30614"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01726">
        <property type="entry name" value="HEQRo_perm_3TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="74"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="7">
        <location>
            <begin position="27"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="227" mass="25336" checksum="242DFA6D251AEB92" modified="2013-07-24" version="1">MWFQNFYDTFYKCFIKEDRYQLLLDGIGVTVKVSLLAVVFGLLIGFLIALCNLSNKKGIRLIGKVYTDIIRGTPTVTQLMIIYFVIFASVHWPKWVIAAIAFGINSGAYVSEIIRAGILSVDKGQTEAGRSLGLNGRQTMIRIVVPQAAKNIFPALCNEFITLIKETAIVGYVGLVDIQKAGDFIKTSTYEAFMPLIGTAIIYFVIIKLLTLALGRLEKRLRKSEVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0U3</accession>
    <name>R5V0U3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phosphoglucosamine mutase</fullName>
            <ecNumber evidence="6">5.4.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">glmM</name>
        <name evidence="11" type="ORF">BN566_01943</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the conversion of glucosamine-6-phosphate to glucosamine-1-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>alpha-D-glucosamine 1-phosphate = D-glucosamine 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:23424"/>
            <dbReference type="ChEBI" id="CHEBI:58516"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="EC" id="5.4.2.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="6">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="PTM">
        <text evidence="6">Activated by phosphorylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.10" evidence="6"/>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01943"/>
    <dbReference type="EnsemblBacteria" id="CCZ84218">
        <property type="protein sequence ID" value="CCZ84218"/>
        <property type="gene ID" value="BN566_01943"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008966">
        <property type="term" value="F:phosphoglucosamine mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05802">
        <property type="entry name" value="GlmM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01554_B">
        <property type="entry name" value="GlmM_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005843">
        <property type="entry name" value="A-D-PHexomutase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005841">
        <property type="entry name" value="Alpha-D-phosphohexomutase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006352">
        <property type="entry name" value="GlmM_bact"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00408">
        <property type="entry name" value="PGM_PMM_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00509">
        <property type="entry name" value="PGMPMM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="SSF53738"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="SSF55957"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01455">
        <property type="entry name" value="glmM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4 6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PGM_PMM_I" evidence="8">
        <location>
            <begin position="3"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="PGM_PMM_II" evidence="9">
        <location>
            <begin position="158"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="PGM_PMM_III" evidence="10">
        <location>
            <begin position="258"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="PGM_PMM_IV" evidence="7">
        <location>
            <begin position="376"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphoserine intermediate" evidence="6">
        <location>
            <position position="101"/>
        </location>
    </feature>
    <feature type="binding site" description="via phosphate group" evidence="6">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Phosphoserine" evidence="6">
        <location>
            <position position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ84218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="450" mass="48487" checksum="F0DC74DF5E887D08" modified="2013-07-24" version="1">MGRLFGTDGARGVAITELTCELAMQIGRAAALVLTKEAKHKAKIVIGKDTRISSDILEAALCAGICSVGADAVQLGVIPTPGVAYLVRSMGADAGVMISASHNSVEFNGIKLFASTGYKLPDAVEEEIEALILDTPEKVTLQSHTNVGRIERYEHAKEDYIQHILESVPTRFDGMKIALDCANGSASATAETLFTALGAEVCMLSAEPDGTNINDHCGSTHMEQLMAYVPAHGCQLGLAFDGDADRCLAVDETGKVVDGDQMIAICAKALKASGKLEKDTAVVTVMTNLGFFHFTKREGIHAVATKVGDRYVLEEMKKGGYCIGGEQSGHIIFLEHATTGDGELSGAKLVEIVKQSGKKLSELAGEMETFPQQMQNVRITADQRNRWKEDEAVCSVMKQYEERLGEDGRILVRESGTEPLIRVMVEAKDADLMQEAVSAIAEELERYTKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0W0</accession>
    <name>R5V0W0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00177</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000195">
        <property type="protein sequence ID" value="CCZ84238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84238">
        <property type="protein sequence ID" value="CCZ84238"/>
        <property type="gene ID" value="BN566_00177"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="385" mass="44071" checksum="9A541233C0B2F2F6" modified="2013-07-24" version="1">MILTSRIAVSNLVIQIAVCYWIALCICFQYIPVSLNRNFVKGYSIRCTRLRTRQGYCLQIAILHIRTILGLIEQAKVERICCVKWRFEGLFYGNLTVLNFVQERNSCNRIFVFIRQFRSAVTTDSTFWHAWIQCFCICSSLWFAHCNIDIVLNHTIFIGFKDIEVLLCRCRNIGKGCGFCFAVLYGNRLDFAAVTCELARICQLAICTIGWCHTICFCGKECKIEGLCIALTAIISLVDNQANGIFHFRNPDTNLCRFCDHRCFYQFILIIQVLIRISKAVPCDVFFASILYVIRNRSLEGNANGLTLIYFKSTRELESQVYDFTGWSILPVHIAGFAHPVASVLIQTRMLIARIRNLYALHFCTRECTSCICGVGQCTDFISHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0Y9</accession>
    <name>R5V0Y9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Addiction module toxin RelE/StbE family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01664</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000173">
        <property type="protein sequence ID" value="CCZ83935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83935">
        <property type="protein sequence ID" value="CCZ83935"/>
        <property type="gene ID" value="BN566_01664"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2310.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035093">
        <property type="entry name" value="RelE/ParE_toxin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004386">
        <property type="entry name" value="Toxin_YafQ-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15738">
        <property type="entry name" value="YafQ_toxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143011">
        <property type="entry name" value="SSF143011"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="49" mass="5684" checksum="8F25B4A42A4A6ECB" modified="2013-07-24" version="1">MKYTLKPTTQFKRDLKKAQKQNKNLDLLNKVLQQLADGIPLPEKTVTML</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>R5V126</accession>
    <name>R5V126_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Radical SAM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_00211</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000209">
        <property type="protein sequence ID" value="CCZ84298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00211"/>
    <dbReference type="EnsemblBacteria" id="CCZ84298">
        <property type="protein sequence ID" value="CCZ84298"/>
        <property type="gene ID" value="BN566_00211"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044271">
        <property type="term" value="P:cellular nitrogen compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018130">
        <property type="term" value="P:heterocycle biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901362">
        <property type="term" value="P:organic cyclic compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901566">
        <property type="term" value="P:organonitrogen compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042364">
        <property type="term" value="P:water-soluble vitamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010722">
        <property type="entry name" value="BATS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024021">
        <property type="entry name" value="FeFe-hyd_HydE_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034422">
        <property type="entry name" value="HydE/PylB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43726">
        <property type="entry name" value="PTHR43726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00348">
        <property type="entry name" value="FeFe_hydrogenase_maturase_(Hyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00876">
        <property type="entry name" value="BATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03956">
        <property type="entry name" value="rSAM_HydE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="44"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ84298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="341" mass="38215" checksum="D909FC3D65E98061" modified="2013-07-24" version="1">MGMEEKIKAGTLNAADWLQILRCSEWDDILRQTADQLRQEVYGKDVYLRGLIEYTNFCKNNCYYCGIRCGNTKAVRYRLSKEEILACCEEGYTLGFRTFVLQGGEDPASTDAWVCSVVSAIRERFPDCAVTLSIGEKKKSSYQAYYNAGARRYLLRHETANPEHYRHLHPAELSLENRKRCLYDLKEIGYQVGSGFMVGSPGQTLSHLVEDLQFLQDLQPDMIGIGPYIAQKETPFAGHPNGTLALTLRLVSLLRILFPYALIPATTALGTIHPQGRELGLQAGANVVMPNLSPVRVRKLYALYDDKICTGEESAQCRHCLEQRVASAGYQVVTAVGDVKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>R5V163</accession>
    <name>R5V163_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pyridoxal 5'-phosphate synthase subunit PdxT</fullName>
            <ecNumber evidence="4">4.3.3.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Pdx2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Pyridoxal 5'-phosphate synthase glutaminase subunit</fullName>
            <ecNumber evidence="4">3.5.1.2</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">pdxT</name>
        <name evidence="7" type="ORF">BN566_00241</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the biosynthesis of pyridoxal 5'-phosphate. The resulting ammonia molecule is channeled to the active site of PdxS.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>H2O + L-glutamine = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>aldehydo-D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = H(+) + 3 H2O + L-glutamate + phosphate + pyridoxal 5'-phosphate</text>
            <dbReference type="Rhea" id="RHEA:31507"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="4.3.3.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; pyridoxal 5'-phosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">In the presence of PdxS, forms a dodecamer of heterodimers. Only shows activity in the heterodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the glutaminase PdxT/SNO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.3.6" evidence="4"/>
    <dbReference type="EC" id="3.5.1.2" evidence="4"/>
    <dbReference type="EMBL" id="CBAR010000214">
        <property type="protein sequence ID" value="CCZ84328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00241"/>
    <dbReference type="EnsemblBacteria" id="CCZ84328">
        <property type="protein sequence ID" value="CCZ84328"/>
        <property type="gene ID" value="BN566_00241"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00245"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036381">
        <property type="term" value="F:pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006543">
        <property type="term" value="P:glutamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042823">
        <property type="term" value="P:pyridoxal phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01749">
        <property type="entry name" value="GATase1_PB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01615">
        <property type="entry name" value="PdxT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002161">
        <property type="entry name" value="PdxT/SNO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021196">
        <property type="entry name" value="PdxT/SNO_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31559">
        <property type="entry name" value="PTHR31559"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01174">
        <property type="entry name" value="SNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005639">
        <property type="entry name" value="Glut_amidoT_SNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03800">
        <property type="entry name" value="PLP_synth_Pdx2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01236">
        <property type="entry name" value="PDXT_SNO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51130">
        <property type="entry name" value="PDXT_SNO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="L-glutamine binding" evidence="4 6">
        <location>
            <begin position="47"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="L-glutamine binding" evidence="4 6">
        <location>
            <begin position="133"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4 5">
        <location>
            <position position="79"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="4 5">
        <location>
            <position position="169"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="4 5">
        <location>
            <position position="171"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4 6">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR005639-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR005639-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ84328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="187" mass="20502" checksum="0641BF023781FACC" modified="2013-07-24" version="1">MKIAVLAVQGAFAEHAKRVQELGAEAVELRQKADVLQSYDGIILPGGESTVQGKLLKELDMFQTIQEQIQGGMPVLATCAGLILLAKEVANDARRCFCTLPVTVQRNAYGRQLGSFHTVAPFAGLGDIPMTFIRAPYIAAVEDGVEVLSTVEERIVAVRYQNQTAVAFHPELDADLRIHEQFLHRCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>R5V178</accession>
    <name>R5V178_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">AraC-type DNA-binding domain-containing proteins</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_00256</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000217">
        <property type="protein sequence ID" value="CCZ84343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00256"/>
    <dbReference type="EnsemblBacteria" id="CCZ84343">
        <property type="protein sequence ID" value="CCZ84343"/>
        <property type="gene ID" value="BN566_00256"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003313">
        <property type="entry name" value="AraC-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037923">
        <property type="entry name" value="HTH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018062">
        <property type="entry name" value="HTH_AraC-typ_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020449">
        <property type="entry name" value="Tscrpt_reg_HTH_AraC-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02311">
        <property type="entry name" value="AraC_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00032">
        <property type="entry name" value="HTHARAC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51215">
        <property type="entry name" value="SSF51215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00041">
        <property type="entry name" value="HTH_ARAC_FAMILY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="4">
        <location>
            <begin position="186"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ84343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="292" mass="34222" checksum="4DCCED90DE2B1E12" modified="2013-07-24" version="1">MNDRDSRENRIHGSEIKPFGYYACEIPNHFHCVPMHWHREFELNFVREGSASFICGEEKFHSAAGDIIIIQPNVMHSIYPRESIHQVYDTLVFRYEIFGGFDSDRAIAACIKPLITGKMHIPTHITTDHPYYAELEMMMNNIFSCAKGDTPQLDLLMRSELLRLFWLLETEATVEHDFYELGEAIRPALEYIAEHFQEPITVKQLADSVHLSESYFMHQFQKQVGFRAVEYLSHYRINQACKELLSTKKSVLEIAFDCGFRNLSNFNRQFRKITGNAPTEYRKKFAEESIFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1Z3</accession>
    <name>R5V1Z3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pseudouridine synthase</fullName>
            <ecNumber evidence="3">5.4.99.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_00409</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Responsible for synthesis of pseudouridine from uracil.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a uridine in RNA = a pseudouridine in RNA</text>
            <dbReference type="Rhea" id="RHEA:48348"/>
            <dbReference type="Rhea" id="RHEA-COMP:12068"/>
            <dbReference type="Rhea" id="RHEA-COMP:12069"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the pseudouridine synthase RluA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.-" evidence="3"/>
    <dbReference type="EMBL" id="CBAR010000243">
        <property type="protein sequence ID" value="CCZ84548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00409"/>
    <dbReference type="EnsemblBacteria" id="CCZ84548">
        <property type="protein sequence ID" value="CCZ84548"/>
        <property type="gene ID" value="BN566_00409"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120159">
        <property type="term" value="F:rRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd02869">
        <property type="entry name" value="PseudoU_synth_RluA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006225">
        <property type="entry name" value="PsdUridine_synth_RluC/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00005">
        <property type="entry name" value="rluA_subfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S4" evidence="4">
        <location>
            <begin position="13"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR606225-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ84548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="327" mass="37193" checksum="F1632B5229F2D0A0" modified="2013-07-24" version="1">MKTFLIQPNDSDQRLDRFLKKMLPSLPNSLLYKAIRKKQIKVNRKRCTGETRLQTGDEIAIFLPDDCLQPAAPAAEKKSVHGNAESLSVCYEDAHLLVLYKPIGMLVHSDAGHADHCLVDNLHAYLQQKGDYHPEQEQSFAPAICNRLDRNTEGLVLAAKEAATLRELNRLIRENRIQKTYLCVLTATPPKQTDILHAYHWKDEKTNTVTISDHPKAGYREIITEYRVLQQQNGLCLAEITLHTGRTHQIRAHMAHIHAPLAGDSKYGDRRKNESLHLKHQLLCAYQLQFHPEADSCLAYLNGKTVTASLPDFVSRFFPTFSSKNRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5V240</accession>
    <name>R5V240_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00292</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000222">
        <property type="protein sequence ID" value="CCZ84385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84385">
        <property type="protein sequence ID" value="CCZ84385"/>
        <property type="gene ID" value="BN566_00292"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="263" mass="28753" checksum="686AD40C93CF0CA6" modified="2013-07-24" version="1">MQKNVMVQDAAGQIIGSTYPKRAKGLLKSGRAVLVDDHTIQLCASAQNTAAPILKKELHMEDLLFCAPAFSTKSRFSTLLTVTDADGDPMELWELDATEETTSATISAQLQQLPKTAYQFRFTLAGSSETERIRCTVQTDTDTKTYALEQGRYLSDSRKDTDGTLLRTFSLPVTSGTDGKVSIQFTVKQACMQILPVQPDAVLQALPAYSYQEWKQDASRAANPIVDLSNAKLPKSALLQILATCGANTTISLENADIYNDME</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5V246</accession>
    <name>R5V246_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00461</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000256">
        <property type="protein sequence ID" value="CCZ84608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00461"/>
    <dbReference type="EnsemblBacteria" id="CCZ84608">
        <property type="protein sequence ID" value="CCZ84608"/>
        <property type="gene ID" value="BN566_00461"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007163">
        <property type="entry name" value="DUF368"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37308">
        <property type="entry name" value="PTHR37308"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04018">
        <property type="entry name" value="DUF368"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="232"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="263"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="295" mass="31901" checksum="58D927A00CF1FE4E" modified="2013-07-24" version="1">MKKIITFIHGFCMALADSVPGVSGGTVAFLLGFYDRFVDSLDDLISGTKEKRIAALRYLIKLGIGWGIGFLSAVLVLANVFESHIYLISSLFMGFILFAIPVVIMEEKQCLKEQISRIFFVLIGIAVVSVITYLTSSGDGANISLTHPNVGTFLYVFVCGAIAICAMVLPGISGSTLLLIFGIYVPIITAIKDFLHLNFSAFPTVFVFGLGVLTGIVAIIKIIRNALDKHRAATVYLIIGLMIGSLYAIVMGPTTLDDPQPHLTIHTFSILFFLIGGVVIIGMQAMKKLSEQKDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5V250</accession>
    <name>R5V250_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00302</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000224">
        <property type="protein sequence ID" value="CCZ84395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84395">
        <property type="protein sequence ID" value="CCZ84395"/>
        <property type="gene ID" value="BN566_00302"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024787">
        <property type="entry name" value="EcsC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12787">
        <property type="entry name" value="EcsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="224" mass="23694" checksum="F6D7E9FDA67A8844" modified="2013-07-24" version="1">MGTETTQIIDLNAAEGKAVQEGEKGWNLLGKLYDGALKGIPGAKSVEALAQEYLSNCGGKKEQAAEQLIRMQVTKCTTTGFLTGLGGLITLPATITADIGSSMYVQIRMIAAIAVMGGYSLQDDVVKSMVFATLLKVEVGNLLKQVGVKTANRASLQLLKKLPGTVLTKINQKLGFRFLTKFGQKGVINLVKVVPVAGGVVNGGLNLVETKAIGKRAMKVFLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5V289</accession>
    <name>R5V289_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Oxidoreductase short chain dehydrogenase/reductase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00511</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000267">
        <property type="protein sequence ID" value="CCZ84658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00511"/>
    <dbReference type="EnsemblBacteria" id="CCZ84658">
        <property type="protein sequence ID" value="CCZ84658"/>
        <property type="gene ID" value="BN566_00511"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008202">
        <property type="term" value="P:steroid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0753">Steroid metabolism</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="266" mass="28361" checksum="28F77EBB85A33514" modified="2013-07-24" version="1">MGFFTKKTVIITGAGRAVLSDGKCGSIGYGIATAFAKEGANLVITGRNVQKLTDAKEELERLYGISVLPVQADVHAGADNAAVVKQVVEQAVAAFGAIHVLINNAQASASGVTLADHTTEQFDLAMYSGLYATFHYMQACYPYLKETQGSVINFASGAGLFGNYGQCAYAAAKEGIRGLTRVAATEWGRDHINVNIVCPLAWTAQLEQFQKAYPDAFEKNVKMPPMGHLGDAEQEIGRVCVQLASPDFKYMTGETITLEGGLGLRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5V2L6</accession>
    <name>R5V2L6_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">30S ribosomal protein S10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpsJ</name>
        <name evidence="6" type="ORF">BN566_00056</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in the binding of tRNA to the ribosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000241">
        <property type="protein sequence ID" value="CCZ84510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00056"/>
    <dbReference type="EnsemblBacteria" id="CCZ84510">
        <property type="protein sequence ID" value="CCZ84510"/>
        <property type="gene ID" value="BN566_00056"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.600">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00508">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001848">
        <property type="entry name" value="Ribosomal_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018268">
        <property type="entry name" value="Ribosomal_S10_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027486">
        <property type="entry name" value="Ribosomal_S10_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036838">
        <property type="entry name" value="Ribosomal_S10_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11700">
        <property type="entry name" value="PTHR11700"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00338">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00971">
        <property type="entry name" value="RIBOSOMALS10"/>
    </dbReference>
    <dbReference type="SMART" id="SM01403">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54999">
        <property type="entry name" value="SSF54999"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01049">
        <property type="entry name" value="rpsJ_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00361">
        <property type="entry name" value="RIBOSOMAL_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal_S10" evidence="5">
        <location>
            <begin position="8"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ84510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="104" mass="11662" checksum="F28802AC7C831870" modified="2013-07-24" version="1">MAVNEKIRIKIKGYEHATVDAAAAKIVEAAKRSGSNVSGPIPLPTDKEIITILRATHKYKDSREQFEMRTHKRLIDVIRPTQKTTEALQKLEIPAGVDIVMELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>R5V310</accession>
    <name>R5V310_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alpha amylase catalytic domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00518</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000267">
        <property type="protein sequence ID" value="CCZ84665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00518"/>
    <dbReference type="EnsemblBacteria" id="CCZ84665">
        <property type="protein sequence ID" value="CCZ84665"/>
        <property type="gene ID" value="BN566_00518"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.400.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="Glyco_hydro_13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045857">
        <property type="entry name" value="O16G_dom_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aamy" evidence="2">
        <location>
            <begin position="134"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="632"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="640"/>
            <end position="660"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="660" mass="76158" checksum="96866848DFF4EE6B" modified="2013-07-24" version="1">MPCMYDSWDLHYKFPFGAIRRRSDCNFTIYLPAGVTPDFPPVMVLFRTGFKERFLPMNANGEQDGCKCYTVTYSPKYAGVHYYYFAYTSNGVRHYIKRGTAHEAALDHGELYQLTVFAKHYETPNFLKGGIMYQIFPDRFCKSGKPHENVPTDRVLRDDWNGTPYYKPDANGHVWNNDYFGGDLEGIRSKLDYLEKLGVTCIYLNPIFESHENHRYNTANYRNVDPLLGTNEDFRVLCEDAKKHGISIILDGVFSHTGADSIYFNKFGRYDTVGAWQSTDSPYYSWYTFFGDHQYEAWWGIDTLPNVVETNPDYLNYICGDGGVLDYWISLGAAGYRLDVADELPDGFLDALRKCVKKNGEEKIVIGEVWEDASNKESYGVKRRYLLGDQLDSVMNYPFREAIINYIKGMDANTFQDWIMGILENYPKQTVDVLMNFVSTHDIERAINRFGGQNCDDKSKDWMAENWLSDAEYKRGKQLLKAAMVLQFFLPGVPSIYYGDEVGLQGWKDPFNRRCFPWGNEDEDLLEYTRELGILRKSSSVFVDGELEFLLTGNELLVFSRFHRNSGESVILILNRSEQTQHFSLHSDVLKNYALSNVASGICKENELVLPAYSYAALFALRTIDRKVEEEEADEEAKEAFDAKEDVTEDEYRDTYKDMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5V326</accession>
    <name>R5V326_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00533</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000267">
        <property type="protein sequence ID" value="CCZ84680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00533"/>
    <dbReference type="EnsemblBacteria" id="CCZ84680">
        <property type="protein sequence ID" value="CCZ84680"/>
        <property type="gene ID" value="BN566_00533"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="61" mass="7102" checksum="37259693B57D1C1F" modified="2013-07-24" version="1">MCSIATFAEAKLVLICKKQYTDMLKTEGFLIPETYQRWYNADPLHKLFVVEILAAYERTDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5V3W3</accession>
    <name>R5V3W3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00599</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000008">
        <property type="protein sequence ID" value="CCZ82794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00599"/>
    <dbReference type="EnsemblBacteria" id="CCZ82794">
        <property type="protein sequence ID" value="CCZ82794"/>
        <property type="gene ID" value="BN566_00599"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05826">
        <property type="entry name" value="Sortase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005754">
        <property type="entry name" value="Sortase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023365">
        <property type="entry name" value="Sortase_dom-sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009835">
        <property type="entry name" value="SrtB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04203">
        <property type="entry name" value="Sortase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63817">
        <property type="entry name" value="SSF63817"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ82794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="281" mass="32381" checksum="53F292ECD2D09CEB" modified="2013-07-24" version="1">MKRNDERRKWLPFFVVLLTLMTVAIVLFFYTCQTTVKVETPAQFPIITETTTTTTTTETTTTTTTTTMTTTTTTATVSVDYTMHIDMTSVKQHKASSPKLVGWIYIPDTVIDYPIMQAEDNNYFLHLDWMEQESFAGCIYEDYRGNIDKTTMTLLYGHNMAAGTMFSNIKYFKDPAWGKSHPYFEVASTEKRYLYEVLSANVLYGESGAEFSYWRPNKGLTMNKEEYLEYLQQIKKTSLVWYGKDDVKEYLDSNVIVLQTCNSGDDDGMRCVIFARCLGER</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5V4J6</accession>
    <name>R5V4J6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00792</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000036">
        <property type="protein sequence ID" value="CCZ82999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82999">
        <property type="protein sequence ID" value="CCZ82999"/>
        <property type="gene ID" value="BN566_00792"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ82999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="103" mass="11745" checksum="7B2533B3453F0405" modified="2013-07-24" version="1">MEPFWEKMGLSTEPQMWRASELLDCLRQHRQDGILIYFFYQDAAYEVGVRKEDGKTVFFLNDDSYPSMLAFCSSANIEGILLSDLLDPIAVFSVNGKAPEAKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5V4R8</accession>
    <name>R5V4R8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Predicted NADH:ubiquinone oxidoreductase subunit RnfG</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_00882</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000054">
        <property type="protein sequence ID" value="CCZ83094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00882"/>
    <dbReference type="EnsemblBacteria" id="CCZ83094">
        <property type="protein sequence ID" value="CCZ83094"/>
        <property type="gene ID" value="BN566_00882"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007329">
        <property type="entry name" value="FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010209">
        <property type="entry name" value="Ion_transpt_RnfG/RsxG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36118">
        <property type="entry name" value="PTHR36118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04205">
        <property type="entry name" value="FMN_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00900">
        <property type="entry name" value="FMN_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0288">FMN</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0830">Ubiquinone</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004406721" evidence="6">
        <location>
            <begin position="39"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="FMN_bind" evidence="7">
        <location>
            <begin position="92"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="184" mass="18794" checksum="E11D03BA4198CA43" modified="2013-07-24" version="1">MASKNNLIKPPVVLAVVCACCCGLLAVANSVTKDKIAAAEAASIQESLQQLPNAGNFTEVSDFQPANNEKATATAVYVDENQQTAVLVTANGYNKGGLQVVIGLDAEGTVTGITFVSVTETPGLGTKVQSNPELLTDHLVGLSDAAAVEQVDGITGATYSSNGMKAAVTCAMDTFAQSKEAILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5V4X6</accession>
    <name>R5V4X6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00947</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000069">
        <property type="protein sequence ID" value="CCZ83164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83164">
        <property type="protein sequence ID" value="CCZ83164"/>
        <property type="gene ID" value="BN566_00947"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008875">
        <property type="entry name" value="TraX"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05857">
        <property type="entry name" value="TraX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="212"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="234" mass="27148" checksum="E381330EBE19CA30" modified="2013-07-24" version="1">MTKLHSLNRNELKYLVIIAMLIDHIAWAFVPTASLLGQIMHIIGRLTGPTMAYMLADGYYYTRSVEKYACRLGIFALLSWIPFSYFELGYIGLQFGVIYTLLLSLLAIWLWDKGKCPQPVKVLGIIGLCILSLFGDWAIFDILYALFFFCYRNDPRKKWTAFCMLTLFCCGNMLATTPWWLAFFQVGIFLVFPLIQFGYNGKSGSKKPFHKWFFYVFYPLHLLILGVLRWVVFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5V571</accession>
    <name>R5V571_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">4Fe-4S ferredoxin iron-sulfur binding domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_01055</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01055"/>
    <dbReference type="EnsemblBacteria" id="CCZ83274">
        <property type="protein sequence ID" value="CCZ83274"/>
        <property type="gene ID" value="BN566_01055"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="31"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ83274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="104" mass="11577" checksum="BCFA58847BB1EBBD" modified="2013-07-24" version="1">MSIAINQNACIGCGKCHDVCPGTLIHLNADRKAFIKYPKDCWGCTSCLKECPVHAIRFYLGADMGGMGSLVHTEKEGDILRWIVERTDGTTTEIRVNQKESNQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5V575</accession>
    <name>R5V575_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Thiamine biosynthesis protein ThiS</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01060</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01060"/>
    <dbReference type="EnsemblBacteria" id="CCZ83279">
        <property type="protein sequence ID" value="CCZ83279"/>
        <property type="gene ID" value="BN566_01060"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016155">
        <property type="entry name" value="Mopterin_synth/thiamin_S_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010035">
        <property type="entry name" value="Thi_S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003749">
        <property type="entry name" value="ThiS/MoaD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02597">
        <property type="entry name" value="ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54285">
        <property type="entry name" value="SSF54285"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01683">
        <property type="entry name" value="thiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="68" mass="7418" checksum="4A8C0CB92DBB8094" modified="2013-07-24" version="1">MKITVAGVTKEVADGLTVAQLIEQEKVETPQYVTVTINDDFVESGTFETTVLKDGDNVEFLYFMGGGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>R5V5T9</accession>
    <name>R5V5T9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">BN566_01253</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000116">
        <property type="protein sequence ID" value="CCZ83484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01253"/>
    <dbReference type="EnsemblBacteria" id="CCZ83484">
        <property type="protein sequence ID" value="CCZ83484"/>
        <property type="gene ID" value="BN566_01253"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022877">
        <property type="term" value="F:protein-N(PI)-phosphohistidine-fructose phosphotransferase system transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00211">
        <property type="entry name" value="PTS_IIA_fru"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05569">
        <property type="entry name" value="PTS_IIB_fructose"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016152">
        <property type="entry name" value="PTrfase/Anion_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002178">
        <property type="entry name" value="PTS_EIIA_type-2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036095">
        <property type="entry name" value="PTS_EIIB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013011">
        <property type="entry name" value="PTS_EIIB_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003501">
        <property type="entry name" value="PTS_EIIB_2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003352">
        <property type="entry name" value="PTS_EIIC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013014">
        <property type="entry name" value="PTS_EIIC_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004715">
        <property type="entry name" value="PTS_IIA_fruc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003353">
        <property type="entry name" value="PTS_IIB_fruc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006327">
        <property type="entry name" value="PTS_IIC_fruc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00359">
        <property type="entry name" value="PTS_EIIA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02378">
        <property type="entry name" value="PTS_EIIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02302">
        <property type="entry name" value="PTS_IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52794">
        <property type="entry name" value="SSF52794"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55804">
        <property type="entry name" value="SSF55804"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00829">
        <property type="entry name" value="FRU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00848">
        <property type="entry name" value="fruA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01427">
        <property type="entry name" value="PTS_IIC_fructo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51094">
        <property type="entry name" value="PTS_EIIA_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00372">
        <property type="entry name" value="PTS_EIIA_TYPE_2_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51099">
        <property type="entry name" value="PTS_EIIB_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51104">
        <property type="entry name" value="PTS_EIIC_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0598">Phosphotransferase system</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="305"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="337"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="375"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="401"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="438"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="516"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="576"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="614"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="domain" description="PTS EIIA type-2" evidence="13">
        <location>
            <begin position="5"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="PTS EIIB type-2" evidence="14">
        <location>
            <begin position="172"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="PTS EIIC type-2" evidence="15">
        <location>
            <begin position="297"/>
            <end position="645"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CCZ83484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="645" mass="67849" checksum="298066683DC3F599" modified="2013-07-24" version="1">MRIVDLLSKDSILLNASPKNKAEAIDLLVDLQVKGGKITDRETYKQGILAREENGSTAVGEGIAIPHAKNAAVKAPSLAAMTVPEGVDYEALDDEPSNLLFMIAAPSDGGDVHLEVLSRLMVILMDEDFRAKLLAAESKEAFLQVIDEMEREKYPDEPQEAPKAAPEADGRIKVLAVTACPTGIAHTYMAAEALEKAGKKLGISIKVETNGSGGAKNILTKEEIEQCDGIIVAADKNVEMARFDGKKVWKTKVSDGIKIPEELIQKIESGDVPVYHHAGGAGESQDSGDNESFGRKMYKHLMNGVSNMLPFTIAGGIFIAIAFLIDTLAGAPQDSNFGTYTAAAAFFKTIGGYAFNFMVPILAAYIGKSIADRPGFLVGLVGGYLATTGSTFAAISGDVPSGFLGALLAGFAGGFLMLGVEKLCDHMPKALNGIKPVLIYPLVGLGMIAILMCAVNPFMGMINTGLSDLLNSMGSSSKILLGCVLGGMMSIDMGGPFNKAAYVFGTMAIASGSYDIMAAVMIGGMVPPIAIALSTTFFKNRWTEDERKNGPVNYIMGLSFVTEGAIPYAAADPLRVIPACVIGAATAGGLSMAFDCTLMAPHGGIFVFAVVGNWPFYLLSLAIGSLVGMLMLAVLKKKKVPATAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5V602</accession>
    <name>R5V602_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcription regulator (Putative)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01288</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000120">
        <property type="protein sequence ID" value="CCZ83519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01288"/>
    <dbReference type="EnsemblBacteria" id="CCZ83519">
        <property type="protein sequence ID" value="CCZ83519"/>
        <property type="gene ID" value="BN566_01288"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005149">
        <property type="entry name" value="Tscrpt_reg_PadR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03551">
        <property type="entry name" value="PadR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PadR" evidence="1">
        <location>
            <begin position="15"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="107" mass="12415" checksum="111DAFD41B22A045" modified="2013-07-24" version="1">MAAFSVSTALLDAVVLACVAQGDTYGYELTHQIQSVFSISESTLYPVLRRLQKNEYLQTYDQPYQGRNRRYYHITDAGRTALEAYCSEWQQYKQQVDQFLTKKEDAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5V6F9</accession>
    <name>R5V6F9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01450</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000154">
        <property type="protein sequence ID" value="CCZ83714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83714">
        <property type="protein sequence ID" value="CCZ83714"/>
        <property type="gene ID" value="BN566_01450"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="304" mass="34620" checksum="0180B557453C8287" modified="2013-07-24" version="1">MINGAACYLSAIIQTGDCKTGFILFRIAAACHNHTSCTAILPDNRQFCQLLFRNRTHNINQIGFQQRQHNLCFRVAEPTVIFNDLWAIRCQHQAEIQTALERSALCLHRTNGRQEDGFHTSICNFLCIIRVRCNCSHTAGIQTLVLVECPLVIHRGHHRNNGFPVCKGKYRNFRPCQKFFYNNRAAAFAEHLVFHHALDSLFCFFQIGCNDNAFSQSQTICFDDGRELIFLLDVCNGCICIVENFILCGRNVILLHQFLGECLAAFDDCSRLIGAKGCKSSCLHGIDHTQYQRVIRCNKDQINR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>R5V6K2</accession>
    <name>R5V6K2_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Translation initiation factor IF-3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">infC</name>
        <name evidence="8" type="ORF">BN566_01484</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 5">IF-3 binds to the 30S ribosomal subunit and shifts the equilibrum between 70S ribosomes and their 50S and 30S subunits in favor of the free subunits, thus enhancing the availability of 30S subunits on which protein synthesis initiation begins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 5">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the IF-3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000161">
        <property type="protein sequence ID" value="CCZ83749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01484"/>
    <dbReference type="EnsemblBacteria" id="CCZ83749">
        <property type="protein sequence ID" value="CCZ83749"/>
        <property type="gene ID" value="BN566_01484"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00080">
        <property type="entry name" value="IF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036788">
        <property type="entry name" value="T_IF-3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036787">
        <property type="entry name" value="T_IF-3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019813">
        <property type="entry name" value="Translation_initiation_fac3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001288">
        <property type="entry name" value="Translation_initiation_fac_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019815">
        <property type="entry name" value="Translation_initiation_fac_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019814">
        <property type="entry name" value="Translation_initiation_fac_3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10938">
        <property type="entry name" value="PTHR10938"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00707">
        <property type="entry name" value="IF3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05198">
        <property type="entry name" value="IF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54364">
        <property type="entry name" value="SSF54364"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55200">
        <property type="entry name" value="SSF55200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00168">
        <property type="entry name" value="infC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00938">
        <property type="entry name" value="IF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4 5" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 4 5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF3_N" evidence="7">
        <location>
            <begin position="31"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="IF3_C" evidence="6">
        <location>
            <begin position="107"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="195" mass="22461" checksum="58D198CD6A9D1C8C" modified="2013-07-24" version="1">MRTFFEKYAFYFCLKIICLEVLAISKQELQINEEIRDREILVIDQNGTKLGTMSARDAQKLAYEQDLDLVKIAPQATPPVCRIMDYGKYCFEQQKREKEAKKNQKVVSIKEIRMFSAIDTHDFETKVNQAKKFLEGGDKLKVSVRFRKRAIAHPQLGEELLNRFKEACAAVGTVEKPAKMEGRSMVMFISPKASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>R5V6L4</accession>
    <name>R5V6L4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chromosome partition protein Smc</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">smc</name>
        <name evidence="5" type="ORF">BN566_01494</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Required for chromosome condensation and partitioning.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="3">Contains large globular domains required for ATP hydrolysis at each terminus and a third globular domain forming a flexible hinge near the middle of the molecule. These domains are separated by coiled-coil structures.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SMC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000161">
        <property type="protein sequence ID" value="CCZ83759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01494"/>
    <dbReference type="EnsemblBacteria" id="CCZ83759">
        <property type="protein sequence ID" value="CCZ83759"/>
        <property type="gene ID" value="BN566_01494"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030261">
        <property type="term" value="P:chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01894">
        <property type="entry name" value="Smc_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024704">
        <property type="entry name" value="SMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010935">
        <property type="entry name" value="SMC_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036277">
        <property type="entry name" value="SMC_hinge_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011890">
        <property type="entry name" value="SMC_prok"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06470">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005719">
        <property type="entry name" value="SMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00968">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75553">
        <property type="entry name" value="SSF75553"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02168">
        <property type="entry name" value="SMC_prok_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMC hinge" evidence="4">
        <location>
            <begin position="523"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="168"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="270"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="459"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="680"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="32"/>
            <end position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="1185" mass="133325" checksum="C060DE8C49BFDD5A" modified="2013-07-24" version="1">MFFKSLELQGFKSFPDRVKLEFEQGLTAVVGPNGSGKSNIGDAIRWVLGEQSTKLLRGNRMEDVIFSGTKRRKPSGFAAVTIQLDNQTGLFGESYGETIAITRKLYRSGESVYQINGKAVRLKDVQELLMDTGMGRDGYAIIGQGRIAEIVSARSTDRRELFEEATGVSKFRYKREEAERNLQAAQENLVRLQDIAATLEGRVEPLRQQAEVAKQFLKLSEQQKKLELSLWMQQLTGLDQEKAALRDTLLQARGNEQNAALDLKQADALLQEQYREMQQTTVRIQNLRESVQQAEETAAQTQADLAVCANEQQHNEQRLIQVAQQQKDAAAAEQTATEQLQTQQAAIQTLQEQKATAVKACQEQEQFQKELAKQLTAAAAVVKEAETKLRTSYGNQNHSFARAESLAAQIETIQAQTVSQTAQAAALQQQLQAAAETEQQTIDAVSAAVEAETAVRVALEAAQQQHKTLAAHLEQQQVQNAQRQMQLQTAQQRLQLLQEMEHNMEGYAGSVKAVLQAEHGKPHGVYGTVSQLLTTESRYSLAIETALGGAMQNIVVADETAAKDWIRYLKTKRAGRATFLPLTAIKGQVLKESAVLQETAVIGLACDLVQYDAKFQNIVFSLLGRIVIAENLDDATRLAQRYGYRFRIVTLDGQAINAGGSFTGGSSQKKGGILTRKNEQLALQQTIQQLQEQVQADAEQFRQQKAAVQKQLEQVQTLQNQLHHEHAEQLLAAARAETAKKQFQQLQQQLSERQQQRKQLSEQVAALQQQHATAKAEWERVQADLTALQAQQQEAKQQQETLSAEQAAVREQLTTRQIAAAKLEQAYLSQQQQIAEQQERLQQCRQQKQQLTEAEAACREQAVQLQQQEQELQTKLTTCREQMTQHRKTIEQLQRIHAEQEQRIRQQQEGIRELSAARERAAAEVAQLETKQHTLEQQRDRIVQQMREQYDVTLSEANQLAEPISNELLAKQQLETLRRQIRALGHVNVSAIEEYQEVSKQWEFLSAQLQDATQAKQELETLIVQLTVEMQRIFTERFAGINQAFQEIFRELFDGGEASLSLSDPDDVLHSGIEIQVAPPGKVIKNLIALSGGEQSFVAIAIYFAILRQRPTPFCIMDEIDAALDEGNVRRYVQYLQRFRDTTQFILITHRRMAMEAADALYGVTMQEDGISRLLHMEQVQEAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5V6W1</accession>
    <name>R5V6W1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Rod shape-determining protein MreD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01579</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000166">
        <property type="protein sequence ID" value="CCZ83844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83844">
        <property type="protein sequence ID" value="CCZ83844"/>
        <property type="gene ID" value="BN566_01579"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="199" mass="22441" checksum="5A392483C764BE61" modified="2013-07-24" version="1">MGKIKPARTTPAQKRNSVVRWIFYFVVIVLCTAFQNAGDYVKPLLLIPVALCIASNVKQGAAVFIGILCGFLLDFSCNRLAGFYAIPMMLICFITAWLYTQFLQQRVWNLLILTAVVSLILGTQDFLFYYVIWGYEHISLVFLKVILPSCGYTLIAALPLYGIFHFIHERLMPNSNRTIEQAIHTTIESYERGSSGGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7I7</accession>
    <name>R5V7I7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Asp-tRNAAsn/Glu-tRNAGln amidotransferase C subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_01775</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + 2 H(+) + L-asparaginyl-tRNA(Asn) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14513"/>
            <dbReference type="Rhea" id="RHEA-COMP:9674"/>
            <dbReference type="Rhea" id="RHEA-COMP:9677"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78515"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) + L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Heterotrimer of A, B and C subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GatC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000182">
        <property type="protein sequence ID" value="CCZ84049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01775"/>
    <dbReference type="EnsemblBacteria" id="CCZ84049">
        <property type="protein sequence ID" value="CCZ84049"/>
        <property type="gene ID" value="BN566_01775"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003837">
        <property type="entry name" value="Asp/Glu-ADT_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036113">
        <property type="entry name" value="Asp/Glu-ADT_sf_sub_c"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02686">
        <property type="entry name" value="Glu-tRNAGln"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141000">
        <property type="entry name" value="SSF141000"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00135">
        <property type="entry name" value="gatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ84049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="88" mass="9918" checksum="D9431DE315771855" modified="2013-07-24" version="1">MVSYLAELGKLAFSPEELEQMAKDMTSIIAIMDTVKEIDITYDAVADNKNVYLNDLREDFSVPSFPTEKILQNAVNDENCFVVPKVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7J7</accession>
    <name>R5V7J7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01790</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000184">
        <property type="protein sequence ID" value="CCZ84064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01790"/>
    <dbReference type="EnsemblBacteria" id="CCZ84064">
        <property type="protein sequence ID" value="CCZ84064"/>
        <property type="gene ID" value="BN566_01790"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="538" mass="62708" checksum="9663CBF20FD07107" modified="2013-07-24" version="1">MNLLDEQYREFVQPTTLTKKLTLNGETKAYPIYRVRLELLYYNDQNDRIATWISQYKSEHGGFQDLSQEAYNAVIEDFIIKSNPASIEKTKNNIKAVNQREPGVILSDGRIVDGNRRFTCLRKLAEEDPNFCWFETIILNIRMTDSKKQIKMLELAIQHGEEKKVDYNPIDRLVGVYQDIVKTRLLTIEEYAESTNETIQEVKKRVENAKLFSEFLQYIGMPEQYYIAREYQVVSIFSDMLPIFKKCKRESDVVTLKEAIFNNILMQTIDDNRKFIRYISQMMETNIFQTYLQKQLEITEKIKEKLSSNPPKNRAELNDFINENPELAEELRNTLDDFILKAKKRATKSKPSQSVSKSFTILKDVDTRIFSSLSDKELESLTADITRLSEKVDFIKGKLKIKNPVETAFEQQPFLQTESTLPVSTPEFKIADRKIIEPMLLCTDMNQVITTPTVCLHFRLAEKLPFQENEASYRLFFLNAKNERISQKIEITLQAEQTQMVTFSLSITAKETICFLAVQSMNDTEDALQQKIPFQILF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7S3</accession>
    <name>R5V7S3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_01884</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000193">
        <property type="protein sequence ID" value="CCZ84159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01884"/>
    <dbReference type="EnsemblBacteria" id="CCZ84159">
        <property type="protein sequence ID" value="CCZ84159"/>
        <property type="gene ID" value="BN566_01884"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009016">
        <property type="entry name" value="Fe_hydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004108">
        <property type="entry name" value="Fe_hydrogenase_lsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027631">
        <property type="entry name" value="FeFe_hydrog_B1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02906">
        <property type="entry name" value="Fe_hyd_lg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53920">
        <property type="entry name" value="SSF53920"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04105">
        <property type="entry name" value="FeFe_hydrog_B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="122"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="168"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ84159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="493" mass="53859" checksum="8E05600F7496E8C5" modified="2013-07-24" version="1">MATNDATIVKIKHEILEEVAKLVFAGKFEEEKDELPLRLMPGPTAKYRCCVYKEREIVRQRVRLAEGRNVEGAPNNLVVQVVRAACEECPISRYVVTDNCQKCMGKACQQSCRFGAIDIGRTRAHINPSLCKECGKCAKACPYNAIADLIRPCKRSCPVNAMTMDEYGVCQIDESKCIQCGHCIHSCPFGAISSKAFLVDVVKALVAGKRVVAMIAPSAEGQFGDGITIASWREALQQVGFADLVEVALGADMTANAEAAEWMEAYQEGKKKTTSCCPAFVNMIEKHYPMLKENISTTVSPMCAVSRKLKAEHPGTITVFIGPCVAKKSEVLEQRLEGNADYVLTFGEIRAIIRAKGVTLKPEPNEQQDGSVFGKRFGDSGGVTAAVLESMKEQGFTEEVNVHKCNGAEECKKALLLMKLGRLPADFVEGMACVGGCVGGPSRHIEPEVTKRNRDKLIGQADDRNISENLHRFADIDSINMHRSASETEEAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5V8D7</accession>
    <name>R5V8D7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Contains cell adhesion domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00291</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000222">
        <property type="protein sequence ID" value="CCZ84384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00291"/>
    <dbReference type="EnsemblBacteria" id="CCZ84384">
        <property type="protein sequence ID" value="CCZ84384"/>
        <property type="gene ID" value="BN566_00291"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003343">
        <property type="entry name" value="Big_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008964">
        <property type="entry name" value="Invasin/intimin_cell_adhesion"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02368">
        <property type="entry name" value="Big_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49373">
        <property type="entry name" value="SSF49373"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004394997" evidence="1">
        <location>
            <begin position="25"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="BIG2" evidence="2">
        <location>
            <begin position="321"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="BIG2" evidence="2">
        <location>
            <begin position="409"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="527" mass="56514" checksum="B53CD29E604FD457" modified="2013-07-24" version="1">MIGGMLLAALGIGSFFTQQTAVTAAEINIPYTDSNQGFTTGIDGTSLCLNLRNANNCICEDIPQDVDCSGYLSVTFSIHGIGTDAYRLQKDGTQGTPYFAELVGVVRSAQDEEILRFCNGEEDDFAPGTVAITGDGTYTVRTELTQDADQILSLAVNTNIDLYRYQEEGNLSKSGITMQVESVTTQQETATKQATARQETPIFAQDFNTTMPMTTTQRATTTTATTPYWYGTTTSTTPYWHWSVNGTEITTTAMTWKTVVRTTKPAQVVTTTKKVVSSRGYYLSALDQSSTIGLGEQLQLFVGNDGSYTVSSSNHQDIIPAWVSFQPDVATVDDTGLVTAVSEGKAVIAAEINNTAVSIEITVAVPTTTTITTEDLTWGTTTKPSAVNVSVRAPQIVTINNIKRIPKEQAQLQLYLLNQPKDSDVVWSSFYPENASVDQNGLVTGLSGGKAVIGAMIDGKVYAITLYVVGEEATTTAKSSSTVTTGTETIKGDINGDGVVGVDDAVILLQYYAKKAAGLNPKWEDLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5V8I9</accession>
    <name>R5V8I9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Flavin reductase domain protein FMN-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00336</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000225">
        <property type="protein sequence ID" value="CCZ84429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00336"/>
    <dbReference type="EnsemblBacteria" id="CCZ84429">
        <property type="protein sequence ID" value="CCZ84429"/>
        <property type="gene ID" value="BN566_00336"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016646">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-NH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002563">
        <property type="entry name" value="Flavin_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01613">
        <property type="entry name" value="Flavin_Reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00903">
        <property type="entry name" value="Flavin_Reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavin_Reduct" evidence="1">
        <location>
            <begin position="6"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="166" mass="18463" checksum="F789B3910F215140" modified="2013-07-24" version="1">MQKNAFHTLSYGVYAVTTMDGERPVGCIANSAMQITSQPPTLAISINQQNYTHDIIQKTGKLAVCVLPEQTDLSVIGTFGFQSSREVEKFAEIAYDQKDGLPVLKQCCAWFTLEVVQQMNAGTHTVFLAKVLDCDRTTAQQPMTYAYYHNVLKMKSPKAAPTYVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>R5V8R7</accession>
    <name>R5V8R7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta</fullName>
            <shortName evidence="1">ACCase subunit beta</shortName>
            <shortName evidence="1">Acetyl-CoA carboxylase carboxyltransferase subunit beta</shortName>
            <ecNumber evidence="1">2.1.3.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">accD</name>
        <name evidence="3" type="ORF">BN566_00393</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the acetyl coenzyme A carboxylase (ACC) complex. Biotin carboxylase (BC) catalyzes the carboxylation of biotin on its carrier protein (BCCP) and then the CO(2) group is transferred by the transcarboxylase to acetyl-CoA to form malonyl-CoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetyl-CoA + N(6)-carboxybiotinyl-L-lysyl-[protein] = malonyl-CoA + N(6)-biotinyl-L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:54728"/>
            <dbReference type="Rhea" id="RHEA-COMP:10505"/>
            <dbReference type="Rhea" id="RHEA-COMP:10506"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:83144"/>
            <dbReference type="ChEBI" id="CHEBI:83145"/>
            <dbReference type="EC" id="2.1.3.15"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Acetyl-CoA carboxylase is a heterohexamer composed of biotin carboxyl carrier protein (AccB), biotin carboxylase (AccC) and two subunits each of ACCase subunit alpha (AccA) and ACCase subunit beta (AccD).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AccD/PCCB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.15" evidence="1"/>
    <dbReference type="EMBL" id="CBAR010000240">
        <property type="protein sequence ID" value="CCZ84494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00393"/>
    <dbReference type="EnsemblBacteria" id="CCZ84494">
        <property type="protein sequence ID" value="CCZ84494"/>
        <property type="gene ID" value="BN566_00393"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00655">
        <property type="reaction ID" value="UER00711"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009317">
        <property type="term" value="C:acetyl-CoA carboxylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003989">
        <property type="term" value="F:acetyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016743">
        <property type="term" value="F:carboxyl- or carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001295">
        <property type="term" value="P:malonyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01395">
        <property type="entry name" value="AcetylCoA_CT_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034733">
        <property type="entry name" value="AcCoA_carboxyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000438">
        <property type="entry name" value="Acetyl_CoA_COase_Trfase_b_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011762">
        <property type="entry name" value="COA_CT_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01039">
        <property type="entry name" value="Carboxyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01070">
        <property type="entry name" value="ACCCTRFRASEB"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00515">
        <property type="entry name" value="accD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50980">
        <property type="entry name" value="COA_CT_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="1" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="1" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="1" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CoA carboxyltransferase N-terminal" evidence="2">
        <location>
            <begin position="30"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="1">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="297" mass="32796" checksum="07FF7CCAB456A371" modified="2013-07-24" version="1">MVVENMLDDLFKTVTKRFYGEEGAHAHEQPTFKCPRCQHTVLLTVFQEAGKVCPNCRYHARLTAKERLQITADANSFVEYDAELESLDPLSFPNYAEKVTQLQEKTGLKDAVLTGECTIQGSRAVIIIMDSHFMMASMGSAVGEKITRAFERAAEKQLPVIAFTASGGARMQEGILSLMQMAKTSAAVQRHNEAGQLYITVLTDPTTGGVTASFASLGDIILAEPKVLIGFAGRRVIEGTIKQHLPDDFQLAEFMLSHGFVDQIVERSKLRKILSSLLQLHGYLPEQETQTEEVQAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5V8U2</accession>
    <name>R5V8U2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative Xre family DNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00398</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000240">
        <property type="protein sequence ID" value="CCZ84499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84499">
        <property type="protein sequence ID" value="CCZ84499"/>
        <property type="gene ID" value="BN566_00398"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="5"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="75" mass="8760" checksum="04E9995057C25EA4" modified="2013-07-24" version="1">MNNRIRDLREDADFTQAQIASYLNMSQTGYSKYETGENDIPTSVWKALADFYQVSVDYLMRRTDVREPYPSVAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5V8V3</accession>
    <name>R5V8V3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00055</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000241">
        <property type="protein sequence ID" value="CCZ84509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00055"/>
    <dbReference type="EnsemblBacteria" id="CCZ84509">
        <property type="protein sequence ID" value="CCZ84509"/>
        <property type="gene ID" value="BN566_00055"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13579">
        <property type="entry name" value="Glyco_trans_4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="2">
        <location>
            <begin position="22"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="194"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="402" mass="45452" checksum="8802A79775097196" modified="2013-07-24" version="1">MKRVLMLSTVASLQDQFNMANIRMLRNLHCDVHVACNFVQGNNTSPQRIAVFQNELAALGVTCHQIDFARSPFHLLQNHRAYQQLKAVLRADTFDCIHCHTPVGGIYGRQAAAAFQIPVIYTAHGFHFFQGAPLWNWLLFYPVEKYYARKTDVLVTINSEDYDRAVRKMHAKQVVRIPGVGLKPGKYRFASRSREEVREALDLPLDAILLISVGELNRNKNHRVIIQAMARLPQLPLYYILCGKGKEAEFLQELAQSLHLSNRVRILGYRQDVCDLLHASDIFCFPSKREGLGMAALEAMEAGLPLVTSNIHGIRDYSLSGETGFACPVSNVEEFAHAIETLSENRNLRKRMGTYNQQAVEAFYQAKTDEIMKSVYTDVLKLDEILPETEPEPVESASSSRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5V901</accession>
    <name>R5V901_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00435</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000247">
        <property type="protein sequence ID" value="CCZ84574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84574">
        <property type="protein sequence ID" value="CCZ84574"/>
        <property type="gene ID" value="BN566_00435"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="52" mass="6078" checksum="364ECFAFA04E8CCE" modified="2013-07-24" version="1">MNKYTVVFKYSSDGEHWCHTSRIIEAESDFSAMEMIKSRYPYVEIVGVRKTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>R5V980</accession>
    <name>R5V980_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UPF0122 protein BN566_00522</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_00522</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 3">Might take part in the signal recognition particle (SRP) pathway. This is inferred from the conservation of its genetic proximity to ftsY/ffh. May be a regulatory protein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the UPF0122 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000267">
        <property type="protein sequence ID" value="CCZ84669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00522"/>
    <dbReference type="EnsemblBacteria" id="CCZ84669">
        <property type="protein sequence ID" value="CCZ84669"/>
        <property type="gene ID" value="BN566_00522"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00245">
        <property type="entry name" value="UPF0122"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007394">
        <property type="entry name" value="UPF0122"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40083">
        <property type="entry name" value="PTHR40083"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04297">
        <property type="entry name" value="UPF0122"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="47"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ84669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="118" mass="13037" checksum="03806204FA4A21F1" modified="2013-07-24" version="1">MAKDLRIILLLDCYGAFLTPRQQEMMTHYYSEDLSLGEIAALTGTTRQAVRDSVKRSEQVLNDMEEKLQFAEKLCALRQNYAAIAQTAETLAANRAEPAVQHACAAICSCVEAGLELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5V9A7</accession>
    <name>R5V9A7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Lipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit MurT</fullName>
            <ecNumber evidence="1">6.3.5.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">murT</name>
        <name evidence="4" type="ORF">BN566_00557</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The lipid II isoglutaminyl synthase complex catalyzes the formation of alpha-D-isoglutamine in the cell wall lipid II stem peptide. The MurT subunit catalyzes the ATP-dependent amidation of D-glutamate residue of lipid II, converting it to an isoglutamine residue.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + H2O + L-glutamine = ADP + beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:57928"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:60033"/>
            <dbReference type="ChEBI" id="CHEBI:62233"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate = ADP + beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-O-P-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate</text>
            <dbReference type="Rhea" id="RHEA:59488"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:60033"/>
            <dbReference type="ChEBI" id="CHEBI:143132"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-O-P-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + NH4(+) = beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:57932"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:62233"/>
            <dbReference type="ChEBI" id="CHEBI:143132"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms a heterodimer with GatD.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MurCDEF family. MurT subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.13" evidence="1"/>
    <dbReference type="EMBL" id="CBAR010000272">
        <property type="protein sequence ID" value="CCZ84704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00557"/>
    <dbReference type="EnsemblBacteria" id="CCZ84704">
        <property type="protein sequence ID" value="CCZ84704"/>
        <property type="gene ID" value="BN566_00557"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140282">
        <property type="term" value="F:carbon-nitrogen ligase activity on lipid II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02214">
        <property type="entry name" value="Lipid_II_synth_MurT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043703">
        <property type="entry name" value="Lipid_II_synth_MurT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013564">
        <property type="entry name" value="MurT_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08353">
        <property type="entry name" value="MurT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0133">Cell shape</keyword>
    <keyword evidence="1" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="1 4" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Mur_ligase_M" evidence="2">
        <location>
            <begin position="52"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="MurT_C" evidence="3">
        <location>
            <begin position="320"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="356"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="449" mass="50272" checksum="B412A25BA15F3237" modified="2013-07-24" version="1">MKTLLILFGKLMVKILKLLGKNAGNLPGCVLWTLTKHRCVSLFTVDCPIIAVTGTNGKTSVTNYVAQLFEKTGANIITNHSGNNLDTGICSLLLQNCDWRGNVKVDYLILETDESHVPVVYSQLHLETLVVLNFFRDQLDRNGEVETLIRKVNDFCKTFTGTLILNGDDPNTARLGRANPNNHNVKYFHVEPYAFATRERFEAGEGKFCPFCATELVYDYYQYSHIGKFHCPKCGYGTETPDTVAEDVNLDVPSFRIGNRTYTTALNSIYHIYNMAAVAAVAKRYQMDPDILQEVLSHYTVNNGRMEAFQMGSNDVTLNLAKNPVGANMTLRVMNEMQGEKELLFVLNDNIADGLDVSWIWDINFSTFRNVTRVVTSGTRAYDIAVRIKCSGYDARNIVVRPDLDEAVAELNTTQGKKFVIANYTAVQPTRSALQRWIAHHGGAAHETN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>R5VGV5</accession>
    <name>R5VGV5_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Mannan endo-1,4-beta-mannosidase</fullName>
            <ecNumber evidence="2">3.2.1.78</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN566_00611</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random hydrolysis of (1-&gt;4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.</text>
            <dbReference type="EC" id="3.2.1.78"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.78" evidence="2"/>
    <dbReference type="EMBL" id="CBAR010000008">
        <property type="protein sequence ID" value="CCZ82806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00611"/>
    <dbReference type="EnsemblBacteria" id="CCZ82806">
        <property type="protein sequence ID" value="CCZ82806"/>
        <property type="gene ID" value="BN566_00611"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008810">
        <property type="term" value="F:cellulase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005102">
        <property type="entry name" value="Carbo-bd_X2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005087">
        <property type="entry name" value="CBM_fam11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002105">
        <property type="entry name" value="Dockerin_1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045053">
        <property type="entry name" value="MAN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31451">
        <property type="entry name" value="PTHR31451"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03425">
        <property type="entry name" value="CBM_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03442">
        <property type="entry name" value="CBM_X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00404">
        <property type="entry name" value="Dockerin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="SSF81296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004395083" description="Mannan endo-1,4-beta-mannosidase" evidence="8">
        <location>
            <begin position="34"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="9">
        <location>
            <begin position="700"/>
            <end position="768"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ82806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="770" mass="85342" checksum="1D59D3D42CA1DA8F" modified="2013-07-24" version="1">MKGLQKFAALCTSVVLGVCGMGSVLAQPLTADAATESTVPEGFVYVDGTHFMCDGEPFYFAGCNSYDMFTLGDGSSNDSTEAIETKFMNKEAIDARMQQMEDNGVRVLRTWGFSNETWHGFELAPGEYNEAEFMLFDYIMYSAQQHNIRVIITLENYWEAYGGIDKKLSWAGAGSGGNHKSRAAYFTNETCKQWYKDYAKHFAERTNYFTGVQYKDDPTVFAWDLMNEPRYQDAGEDSTGLTLRAWVDEMGAYIKEVDPNHMVYAGLEGHGVDYGFGGDEGNPFVYIQQSPYIDFCSAHPYPDEHWANMTPEDTKNTMVQWIEDAHQEVGKPFVVTEFNVHSSLPEAEYEDYWKAVFDTIEEYDAAGGLFWEFNDRKLSEFTVMDGDPILTYFQQHAARMEDKTPMNSLYTKNTVVDKDNPIDVLLSYEMQSGTVTGLQLDDTILTAGTDYEQTEDTITVSASVFSDVATGEHTIQLLTSEGNQPKVKIRVYSAAEEAQKRSVIDDFESYGEDTALAAAYTTNVNGDTLKISLDAEHTKNGSYAMKYDYSVADGGAGYCGATKKLSNADWTGFDGVRFWILSDGSNRETTFQFVDGAGAYWESIQKVTAETGWQEVKIPFSDFHVQQWGTAAETPTLQGVSEFSIYTGQNGNPGTGVWYFDDIGLYRAGSTTTTTTTTTGTTTTTTTTTTTAETTTDADTDTNYGDVNLDGKVDLVDAIMINKYLAGQITLSEQAAKNADVNADGSLGDGDSTILMRFILMVIPNLPYVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>R5VH12</accession>
    <name>R5VH12_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glyceraldehyde-3-phosphate dehydrogenase</fullName>
            <ecNumber evidence="8">1.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN566_00661</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.-" evidence="8"/>
    <dbReference type="EMBL" id="CBAR010000012">
        <property type="protein sequence ID" value="CCZ82856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00661"/>
    <dbReference type="EnsemblBacteria" id="CCZ82856">
        <property type="protein sequence ID" value="CCZ82856"/>
        <property type="gene ID" value="BN566_00661"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016620">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020831">
        <property type="entry name" value="GlycerAld/Erythrose_P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020830">
        <property type="entry name" value="GlycerAld_3-P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020829">
        <property type="entry name" value="GlycerAld_3-P_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020828">
        <property type="entry name" value="GlycerAld_3-P_DH_NAD(P)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006424">
        <property type="entry name" value="Glyceraldehyde-3-P_DH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43148">
        <property type="entry name" value="PTHR43148"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02800">
        <property type="entry name" value="Gp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00044">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000149">
        <property type="entry name" value="GAP_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00078">
        <property type="entry name" value="G3PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00846">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01534">
        <property type="entry name" value="GAPDH-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00071">
        <property type="entry name" value="GAPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gp_dh_N" evidence="9">
        <location>
            <begin position="3"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Glyceraldehyde 3-phosphate binding" evidence="4">
        <location>
            <begin position="159"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Glyceraldehyde 3-phosphate binding" evidence="4">
        <location>
            <begin position="220"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="160"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="12"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="326"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="site" description="Activates thiol group during catalysis" evidence="6">
        <location>
            <position position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ82856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="345" mass="36567" checksum="F06944D60FD69A7A" modified="2013-07-24" version="1">MSVKVAINGFGRIGRLAFRQMFGAEGYEVVAINDLTKPSMLAHLLKYDTAQGGYAGRIGENKHTVSADDEAGTITVDGKTLTIYAKANAAELPWGEIGVDVVLECTGFYTSKAKAQAHIDAGAKKVVISAPAGNDLPTIVYNVNHETLTTDDKIISAASCTTNCLAPMAKALNDYAPIQSGIMSTIHAYTGDQMILDGPHRKGDLRRARAGAANIVPNSTGAAKAIGLVIPELNGKLIGSAQRVPVPTGSTTILTAVVKGTDVTKEGINAAMKAAATESYGYNEEPIVSSDVIGMKYGSLFDATQTMVSKIADDLYEVQVVSWYDNENSYTSQMVRTIKYFAELA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5VHS3</accession>
    <name>R5VHS3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Radical SAM core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_00839</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000042">
        <property type="protein sequence ID" value="CCZ83046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00839"/>
    <dbReference type="EnsemblBacteria" id="CCZ83046">
        <property type="protein sequence ID" value="CCZ83046"/>
        <property type="gene ID" value="BN566_00839"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034457">
        <property type="entry name" value="Organic_radical-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023821">
        <property type="entry name" value="rSAM_TatD-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023822">
        <property type="entry name" value="rSAM_TatD-assoc_bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30352">
        <property type="entry name" value="PTHR30352"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01111">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04100">
        <property type="entry name" value="rSAM_pair_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04038">
        <property type="entry name" value="tatD_link_rSAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="10"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="203" mass="22622" checksum="C39C801E40D45FF6" modified="2013-07-24" version="1">MKQTAMTIAYPVGKNLYLNLTNRCPCACTFCIRTMGSSAYGSDPLWLEHEPSWEEVKAALDAVDVCSYEEVVFCGFGEPTERLELLCKTAAYLKEQGVRCIRLNTNGLSDLLHERKTAQDLHGLVDIVSISLNAGTEEEYRKVTRPKFPNAFAALQEFAADCKDSVPTIMYSVVDILPKAELDAAQALADRQGIPLRVRKFEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-09-29" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>R5VHV6</accession>
    <name>R5VHV6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Leucine rich repeat protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00893</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000059">
        <property type="protein sequence ID" value="CCZ83106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83106">
        <property type="protein sequence ID" value="CCZ83106"/>
        <property type="gene ID" value="BN566_00893"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002105">
        <property type="entry name" value="Dockerin_1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026906">
        <property type="entry name" value="LRR_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00404">
        <property type="entry name" value="Dockerin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13306">
        <property type="entry name" value="LRR_5"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Dockerin" evidence="3">
        <location>
            <begin position="966"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="445"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="1039" mass="113364" checksum="259D2DBE3DCB5AA0" modified="2013-07-24" version="1">MKKWHQKSIAIFIAATCLLAGGTIGLSQESLSKLQLTAAAETENTNLVGASFGLELEYDYLDDGTLEITKFVSSYSTDVELPSTIEGKQITSIGHSAFSGCSDITSITIPNGVTSIGYWAFEKCSNLTSIVIPDSVKTIGGYAFSNCSSLTDITIPDGVTTIGYSAFADCRKLTSINIPESVTRIGNSAFSNCNSLETFMVDPENPSYTSEDGVLFNKDLTTLLYYPKGKSGAYTIPDGAINIGESAFRGCSNLTDVIIPDSVTTIGASAFSDCINLTSIYLSSGVSNIANTSFSNCSGLEAFIVDAANTSYTSQDGVLFDESMETLLCYPQGKTGTYTIPTFVTSIGYSAFRSCEGLTSVTIPESVTAIGDWAFRQCSNLTSVTLPNSVASIGENAFGYCSQLTDLYYNGTEAEWNEISVGYDAVASSVSIHYEPVVTTTTTTTTMTTTTTTTTTTITTEPETTTESETTTESETTTTTQPTTTTTEPSTATTITTTTTTNVYTFIPEADKWNFLNSGYSFGGYDKTYRDGISDSDYSKLMNGLTNIEKARVNGLLYQNWSGSCYGMAVTSILAANGILNPSDWQDGATFLSDIDAPPSAEVKSLINYYMALQKTNVVENKSKTFLFFTEKEKLQILVNCLKDGSPTLLTFWGTASNGNEWGHAVVAYDLQYGTYSINGKAYNGKVLIYDNNATQLSDDKYDLYFNSSKGTWTIPHYSDANRLGFISDYIGFINYHGYIDTQDDPITADPFIASLQSNSLESNYALTKINFNQDKNSYTFCATEADEIRTFSDFTDNVDENNGDLNFAMRDATKGYELKLDGTEDVDLSMWYENSAFFVTGSNSNVAVFAPSGYVSLKGSDTDYHFNMLYNDGYHTTDWYSFQVEGNHVDAANMKQTEEGYVLHADNLNNVTAQAYNDDANAEITFSTDYTDVLLYEIDEETIGVAVDEDGDGVYETTIAQSDADDTAYGDLNTDNKVDLRDIIVLNKYLANMIQLSDTQMENADCYKDGSVDEKDATSLMKFVLFLTNKLPEYPART</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>R5VI01</accession>
    <name>R5VI01_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Diadenylate cyclase</fullName>
            <shortName evidence="10">DAC</shortName>
            <ecNumber evidence="10">2.7.7.85</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Cyclic-di-AMP synthase</fullName>
            <shortName evidence="10">c-di-AMP synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">dacA</name>
        <name evidence="12" type="ORF">BN566_00959</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the condensation of 2 ATP molecules into cyclic di-AMP (c-di-AMP), a second messenger used to regulate differing processes in different bacteria.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>2 ATP = 3',3'-c-di-AMP + 2 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:35655"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:71500"/>
            <dbReference type="EC" id="2.7.7.85"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Probably a homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the adenylate cyclase family. DacA/CdaA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.85" evidence="10"/>
    <dbReference type="EMBL" id="CBAR010000069">
        <property type="protein sequence ID" value="CCZ83176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00959"/>
    <dbReference type="EnsemblBacteria" id="CCZ83176">
        <property type="protein sequence ID" value="CCZ83176"/>
        <property type="gene ID" value="BN566_00959"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106408">
        <property type="term" value="F:diadenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006171">
        <property type="term" value="P:cAMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019932">
        <property type="term" value="P:second-messenger-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1700.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01499">
        <property type="entry name" value="DacA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014046">
        <property type="entry name" value="C-di-AMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034701">
        <property type="entry name" value="CdaA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045585">
        <property type="entry name" value="CdaA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036888">
        <property type="entry name" value="DNA_integrity_DisA_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003390">
        <property type="entry name" value="DNA_integrity_scan_DisA_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19293">
        <property type="entry name" value="CdaA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02457">
        <property type="entry name" value="DAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004793">
        <property type="entry name" value="UCP004793"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143597">
        <property type="entry name" value="SSF143597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00159">
        <property type="entry name" value="TIGR00159"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51794">
        <property type="entry name" value="DAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 10" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="37"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="66"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="DAC" evidence="11">
        <location>
            <begin position="107"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CCZ83176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="311" mass="34445" checksum="32B1D8F715775F01" modified="2013-07-24" version="1">MQNGEGCDNKPVRWTELMTWCRDAINSLFSILKTTELVDVFDILVLSYLVYWVIKLVRETRAGQLMKGIVLLFIAYIIAKFLQMKVVSYLMEQAFSIGVIALMIMFQPELRRALEKVGNSKWGLSFGLSTPTGDAAVWGTAIEAICDACVELSATSTGALIVVERQTRLGEQIDNGTILNATPSKELFGNIFYPKTPLHDGAVIMRDGMILAAACFLPKPQKEELVNKKLGSRHRAAIGMSENSDAIVIVVSEETGSISVAENGLLKSGFTREQLKQLLTDRLIQTDTDSAIRKAARKIPFIKSKKEKEQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5VI41</accession>
    <name>R5VI41_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00121</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000079">
        <property type="protein sequence ID" value="CCZ83226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83226">
        <property type="protein sequence ID" value="CCZ83226"/>
        <property type="gene ID" value="BN566_00121"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="177" mass="19923" checksum="EF2A51668CC1CA97" modified="2013-07-24" version="1">MVVTTTTAAPASTDTENSDQAPETYQSVLSEYYTAFIQNQPDTLYRLMAPSAYWDYYMEHYDKTKEDVISTYQQAITNTMASWKQQCGDDVTVSFAIKGMSEQSQDFLTEWTDDMNEMIGEDKVHAQDAVTLNVERTLTGSKGTQTETISPTLIQVDGLWYILQENTTAADSQQATE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>R5VIP5</accession>
    <name>R5VIP5_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA pseudouridine synthase B</fullName>
            <ecNumber evidence="4">5.4.99.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">tRNA pseudouridine(55) synthase</fullName>
            <shortName evidence="4">Psi55 synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">tRNA pseudouridylate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">tRNA-uridine isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">truB</name>
        <name evidence="7" type="ORF">BN566_01187</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>uridine(55) in tRNA = pseudouridine(55) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:42532"/>
            <dbReference type="Rhea" id="RHEA-COMP:10101"/>
            <dbReference type="Rhea" id="RHEA-COMP:10102"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.25"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the pseudouridine synthase TruB family. Type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.25" evidence="4"/>
    <dbReference type="EMBL" id="CBAR010000097">
        <property type="protein sequence ID" value="CCZ83411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01187"/>
    <dbReference type="EnsemblBacteria" id="CCZ83411">
        <property type="protein sequence ID" value="CCZ83411"/>
        <property type="gene ID" value="BN566_01187"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106029">
        <property type="term" value="F:tRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02573">
        <property type="entry name" value="PseudoU_synth_EcTruB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01080">
        <property type="entry name" value="TruB_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002501">
        <property type="entry name" value="PsdUridine_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014780">
        <property type="entry name" value="tRNA_psdUridine_synth_TruB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032819">
        <property type="entry name" value="TruB_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13767">
        <property type="entry name" value="PTHR13767"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16198">
        <property type="entry name" value="TruB_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01509">
        <property type="entry name" value="TruB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00431">
        <property type="entry name" value="TruB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TruB_N" evidence="5">
        <location>
            <begin position="23"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="TruB_C_2" evidence="6">
        <location>
            <begin position="174"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF16198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ83411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="297" mass="32631" checksum="5E0D93967B719197" modified="2013-07-24" version="1">MNGILCMNKPQDFTSFDVIGKLRGILHMKRLGHTGTLDPMATGVLPILVGTATKACDILPNQDKTYQATVVFGKATDTLDIWGKPLQDYPEQHVTEAALRAILPEFLGDITQLPPMYSAVSVNGKRLYELARKGETVERPTRTVHIDAITLDAFDETQQTATLTVSCGKGTYIRTLLSDIGQRLGGDAVMTALTRTAACGYPLQECLTFEQVAAAMADGTLEEHLLPTDSLFSSYPKLRLNAAQERMFCNGVKLDLNRLRNLQPDQDIYTVYGATGTFLGTALADRTQQELRIGKRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5VIR7</accession>
    <name>R5VIR7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">4-O-beta-D-mannosyl-D-glucose phosphorylase</fullName>
            <shortName evidence="4">MGP</shortName>
            <shortName evidence="4">Mannosylglucose phosphorylase</shortName>
            <ecNumber evidence="4">2.4.1.281</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_01200</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Converts 4-O-beta-D-mannopyranosyl-D-glucopyranose (Man-Glc) to mannose 1-phosphate (Man1P) and glucose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate = alpha-D-mannose 1-phosphate + D-glucose</text>
            <dbReference type="Rhea" id="RHEA:32531"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
            <dbReference type="ChEBI" id="CHEBI:64351"/>
            <dbReference type="EC" id="2.4.1.281"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the glycosyl hydrolase 130 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.281" evidence="4"/>
    <dbReference type="EMBL" id="CBAR010000102">
        <property type="protein sequence ID" value="CCZ83426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01200"/>
    <dbReference type="EnsemblBacteria" id="CCZ83426">
        <property type="protein sequence ID" value="CCZ83426"/>
        <property type="gene ID" value="BN566_01200"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.115.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00928">
        <property type="entry name" value="Man_Glc_phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028583">
        <property type="entry name" value="Man_Glc_phosphorylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007184">
        <property type="entry name" value="Mannoside_phosphorylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34106">
        <property type="entry name" value="PTHR34106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04041">
        <property type="entry name" value="Glyco_hydro_130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016202">
        <property type="entry name" value="PH1107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="SSF75005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="1 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="393" mass="44469" checksum="27C9D74D9C8D0156" modified="2013-07-24" version="1">MMQERYEQQLRKQEALLNRKNEKTDFYNGIYDRWKYPVLTREHVPLYWRYDLNPETNPYFMERLGINAVFNAGAIELDGKICLVARVEGSDRKSFFAVAESTTGVDQFVFREKPVQLPDCDPKETNVYDMRLTKHEDGWIYGVFCSESSANDPKDLSAAVAQAGIVRTKDLNTWERLPNLITKQSPQQRNVCLLPKFVDGKYAFYTRPMDGFIDTGSGGGIGYGLVDDILHPVIETEKMTSMRKYHTITEAKNGSGATPIATEKGWLHIAHGVRNTAAGLRYVIYIFVTDLEDPSRVIAEPSGYLIAPFGAERVGDVSNVVFTNGAVVREDGTVYIYYASADTRLHVATSTIDQLLDYAFHTPTDPLRSVDCVKQRCELIDRNLAFLQKEQGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5VIU9</accession>
    <name>R5VIU9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Galactose-1-phosphate uridylyltransferase</fullName>
            <shortName evidence="10">Gal-1-P uridylyltransferase</shortName>
            <ecNumber evidence="10">2.7.7.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">UDP-glucose--hexose-1-phosphate uridylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">galT</name>
        <name evidence="13" type="ORF">BN566_01220</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>alpha-D-galactose 1-phosphate + UDP-alpha-D-glucose = alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose</text>
            <dbReference type="Rhea" id="RHEA:13989"/>
            <dbReference type="ChEBI" id="CHEBI:58336"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="EC" id="2.7.7.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 10">Carbohydrate metabolism; galactose metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the galactose-1-phosphate uridylyltransferase type 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.12" evidence="10"/>
    <dbReference type="EMBL" id="CBAR010000105">
        <property type="protein sequence ID" value="CCZ83446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01220"/>
    <dbReference type="EnsemblBacteria" id="CCZ83446">
        <property type="protein sequence ID" value="CCZ83446"/>
        <property type="gene ID" value="BN566_01220"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00214"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008108">
        <property type="term" value="F:UDP-glucose:hexose-1-phosphate uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006012">
        <property type="term" value="P:galactose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00571">
        <property type="entry name" value="GalP_UDP_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000766">
        <property type="entry name" value="GalP_uridyl_Trfase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023425">
        <property type="entry name" value="GalP_uridyl_Trfase_II_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005850">
        <property type="entry name" value="GalP_Utransf_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005849">
        <property type="entry name" value="GalP_Utransf_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39191">
        <property type="entry name" value="PTHR39191"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02744">
        <property type="entry name" value="GalP_UDP_tr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01087">
        <property type="entry name" value="GalP_UDP_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006005">
        <property type="entry name" value="GalT_BS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01163">
        <property type="entry name" value="GAL_P_UDP_TRANSF_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 10" id="KW-0299">Galactose metabolism</keyword>
    <keyword evidence="7 10 13" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GalP_UDP_transf" evidence="11">
        <location>
            <begin position="59"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="GalP_UDP_tr_C" evidence="12">
        <location>
            <begin position="248"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CCZ83446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="498" mass="55928" checksum="A447CABD678629C0" modified="2013-07-24" version="1">MICDAIQRLINYGITQDLITKADQFVVQNQLMEALQLTDWQLSGAETEPDASIDAILQPLIEYACSNGIIADTAASRDLFDTKLMGILTPMPHEMIAKFQERYQESPEAATDWYFDISQKLNYVRAGRIAKDKKWKYASEYGMLDITINRSKPEKDPRDIAAARNQKAAAYPKCQLCPENAGFAGNSNHPARQNLRPIPMQIDGQNWELQYSPYGYYHEHCIVFNDAHVPMKVDHAIFEKLFDVLDFLPHYFIGSNADLPIVGGSILSHEHFQGGHYTFPMAVAPIEIPVSLADYPNVQAGILKWPMSVVRLSSTNRKELSDACNTILEQWRSYSDATVGIYAETDGTPHNTITPIARKKGDAYECDLVLRNNRTSEDRPLGIFHPNPSLHHIKKENIGLIEVMGLAVLPARLAVELDVLKEAMLQHEDLTVNPQTASHAVWAAEVQKQHPEFCEENAEAILQAEVGKVFEQVLCDAGVFKRDEAGKTAFLKFLQIFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VIV6</accession>
    <name>R5VIV6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01225</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000108">
        <property type="protein sequence ID" value="CCZ83451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83451">
        <property type="protein sequence ID" value="CCZ83451"/>
        <property type="gene ID" value="BN566_01225"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="113" mass="12766" checksum="CEDACC13522FDC44" modified="2013-07-24" version="1">MQDVDGNYDKFSLTNPQAVAVYQLRHKQIAFDRSYLYNPQKALEFRSAASQNGWMQQFFTLAGWIACDLNDCLSVTDEIENARISAIVWNGSQTGISTTISLYSDVFQQKGTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJA4</accession>
    <name>R5VJA4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nucleotide-binding protein BN566_01359</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01359</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Displays ATPase and GTPase activities.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RapZ-like family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000144">
        <property type="protein sequence ID" value="CCZ83596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01359"/>
    <dbReference type="EnsemblBacteria" id="CCZ83596">
        <property type="protein sequence ID" value="CCZ83596"/>
        <property type="gene ID" value="BN566_01359"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00636">
        <property type="entry name" value="RapZ_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005337">
        <property type="entry name" value="RapZ-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30448">
        <property type="entry name" value="PTHR30448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03668">
        <property type="entry name" value="ATP_bind_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005052">
        <property type="entry name" value="P-loopkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="8"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="59"/>
            <end position="62"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="289" mass="33392" checksum="7A82F3720169FE5E" modified="2013-07-24" version="1">MQVVVVTGMSGSGKSTVMDVMEDIGYYCIDNLPPQLIHRFVEICRESENKLEKIAIAADLRSGEMFADAYDCVCRLKEQSDLHVKLLYIEAQDEVIIKRYKETRRKHPLDERFNGCLHQALEFEREQLLRLKAIADYYIETSYISVSQLKEQIRETFLETPSDSLSIKVTSFGFKYGVSTESDLVFDVRCLPNPYYIPELRHHTGCDACVRDYVMQFEQSKILLQKIEDLLDFLIPLYIKEGKSRLVIAFGCTGGKHRSITFTELIGDYLIEKGMHVIKQHRDIGKDRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJP3</accession>
    <name>R5VJP3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribosomal silencing factor RsfS</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rsfS</name>
        <name evidence="3" type="ORF">BN566_01501</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Functions as a ribosomal silencing factor. Interacts with ribosomal protein L14 (rplN), blocking formation of intersubunit bridge B8. Prevents association of the 30S and 50S ribosomal subunits and the formation of functional ribosomes, thus repressing translation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with ribosomal protein L14 (rplN).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the Iojap/RsfS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000161">
        <property type="protein sequence ID" value="CCZ83766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01501"/>
    <dbReference type="EnsemblBacteria" id="CCZ83766">
        <property type="protein sequence ID" value="CCZ83766"/>
        <property type="gene ID" value="BN566_01501"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:mature ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090071">
        <property type="term" value="P:negative regulation of ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01477">
        <property type="entry name" value="Iojap_RsfS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004394">
        <property type="entry name" value="Iojap/RsfS/C7orf30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21043">
        <property type="entry name" value="PTHR21043"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00090">
        <property type="entry name" value="rsfS_iojap_ybeB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="2" id="KW-0810">Translation regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="120" mass="13746" checksum="3EF8ADEA541EBA6A" modified="2013-07-24" version="1">MEQKAVLEAIVKALDSKRAEDIQVIGVKNLTILGDYFVIANGTSTTHTKTLADEVEYQMTKLGIEPLHREGRGNGSNWIVLDYNDIIIHVFYKETRAFYQLERLWADGEKMDVTAWLKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJQ4</accession>
    <name>R5VJQ4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ferredoxin--NADP+ reductase subunit alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01511</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="2">Binds 1 [2Fe-2S] cluster per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="1">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000161">
        <property type="protein sequence ID" value="CCZ83776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01511"/>
    <dbReference type="EnsemblBacteria" id="CCZ83776">
        <property type="protein sequence ID" value="CCZ83776"/>
        <property type="gene ID" value="BN566_01511"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006221">
        <property type="term" value="P:pyrimidine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012165">
        <property type="entry name" value="Cyt_c3_hydrogenase_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019480">
        <property type="entry name" value="Dihydroorotate_DH_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10418">
        <property type="entry name" value="DHODB_Fe-S_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006816">
        <property type="entry name" value="Cyc3_hyd_g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="SSF52343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="SSF63380"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="1" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="3">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="62"/>
            <end position="64"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR006816-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR006816-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="276" mass="30155" checksum="F6E8593D300F65D5" modified="2013-07-24" version="1">MFPIIEKTVLNPSVVKIKLDAPLIAKKVLPGQFIIFRIDEYGERVPLTVADYDREAGTITLIFQIVGHTTERLAALNAGDSILDLVGPLGVATEIPEGVESVCVVGGGVGCAIAYPQAKQLFHMGKKVDVIAGFRSKDIVILEDEFRQCSDNLFLCTDDGTYGQKGFVTNVLQEQIDAGKKYDMVIAIGPIPMMKFVSKVTEPYAIPTIVSLNPIMVDGTGMCGGCRVTVDGQIRYACVDGPDFDGHKVDYDELMRRGGTYREQEHACRMMNAEVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJR3</accession>
    <name>R5VJR3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01521</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000162">
        <property type="protein sequence ID" value="CCZ83786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83786">
        <property type="protein sequence ID" value="CCZ83786"/>
        <property type="gene ID" value="BN566_01521"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dockerin" evidence="2">
        <location>
            <begin position="142"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="179"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="219" mass="23725" checksum="D1F0126FA977CF80" modified="2013-07-24" version="1">MKTTYFVSASEQDAEKFYQLPGVEAVYEVRSEAHHSAWIGDGTSTSINVTVKVEKGTDFGVEQCADLPYTVSSVTEIESTDDAMDAYKLVVKVPDGKIYKAALDMIRTLLEEDIVPDASVSYMTTALALVGNPVLKEVPNHYLAANSDLDGDGTVDVQDAVELLTYYARKAANLPASFSHLDDQEAALQLADVNKDGNIDTADAIEILTYYTKQAAGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJU8</accession>
    <name>R5VJU8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01551</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000165">
        <property type="protein sequence ID" value="CCZ83816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01551"/>
    <dbReference type="EnsemblBacteria" id="CCZ83816">
        <property type="protein sequence ID" value="CCZ83816"/>
        <property type="gene ID" value="BN566_01551"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025374">
        <property type="entry name" value="DUF4364"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14277">
        <property type="entry name" value="DUF4364"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="203" mass="23895" checksum="808EB32342BCA18E" modified="2013-07-24" version="1">MQDENLMKMSQIATPPLMDTHMANILICCLLDHLKKPVTPTQLYEIAVETEIINYFFYQESITYLQKNGSLQLKQDENGQDWYSLTKVGEECAKELKEYVAKPYRDKIISEAKQYFIREKRREEVKISYLPLKKGYHVQVRYLDLQSDLMDLKLYAPDMKQAKYLGTQIMRNPANFYGKVLEAVFSNKEEVKKQPPQPPKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5VK34</accession>
    <name>R5VK34_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Penicillin-binding protein transpeptidase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_01630</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transpeptidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000171">
        <property type="protein sequence ID" value="CCZ83901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01630"/>
    <dbReference type="EnsemblBacteria" id="CCZ83901">
        <property type="protein sequence ID" value="CCZ83901"/>
        <property type="gene ID" value="BN566_01630"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008658">
        <property type="term" value="F:penicillin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005543">
        <property type="entry name" value="PASTA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005311">
        <property type="entry name" value="PBP_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036138">
        <property type="entry name" value="PBP_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001460">
        <property type="entry name" value="PCN-bd_Tpept"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03793">
        <property type="entry name" value="PASTA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03717">
        <property type="entry name" value="PBP_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00905">
        <property type="entry name" value="Transpeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00740">
        <property type="entry name" value="PASTA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56519">
        <property type="entry name" value="SSF56519"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51178">
        <property type="entry name" value="PASTA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="PASTA" evidence="4">
        <location>
            <begin position="647"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="domain" description="PASTA" evidence="4">
        <location>
            <begin position="710"/>
            <end position="775"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="780" mass="85524" checksum="03BEB102A60E780A" modified="2013-07-24" version="1">MDNKPYDKLRASGVNAPTAKMKFRTNVVIQIAMMLFACAIIINLFKVSVVQNKKYEALANNYHFGTMRLEAQRGAIYDATGTPLAWSATVYNVYIDPQLFREEMDDVQKNNESKQAAAEKNGKTATDIVDVATLRENIATYLAGKLNLQKADIEKAFDADGRYYILQTQVEKNVADEIENYFDNLNLVSFATEATTRRYYPQEELAASVIGFTNGDGDGQYGLEYQYNNYLAGVDGRIVSAQAANGEEMPYRYSTTYEAQNGSSLYLTLDTTMQYYLEKAIGEMAVKFNVQQRACGIIMNPKTGAIYAMATYPSFDLNNPSDIYDTATSRALDSLSGQEYSDAYLAARETQWKNKAISEIYYPGSVFKVFTSAAAFEENLIDLQNDSFYCSGYFTVMGEKIGCSNRSGHGTQTFTQALTNSCNPAFMEIGLRLGVEKFSYYFKGFGLTEKTGIDLPGEVASLYIPEDTMSNVDLASSSFGQANKITPIQMITAYSAAINGGKLVTPYLVQEVVDADGNIVMEHETEEKRQVISEETSKTIREQLEAVVEGNGGHNAYIQGYRIGGKSGTSEKLDEYTEGQEMKYVASYGCFAPADDPEVIMLIMADEPDNSIAYYGSTVVVPYARTVMSEILPYLGFYPEYTDEEYADRNVTVPLVQEKSLDSATATLDDMGLSYEVVGEGSSVVSQCPKTGAVIEKGGKVILYTEENTEPEVVEVPNLVGLSAAEANTALTQLGLNIVTMGASSDNADAKVQFQSEPAGTSVEVGTVINLTMSINDQSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5VKE2</accession>
    <name>R5VKE2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sporulation protein YlmC/YmxH family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01715</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000178">
        <property type="protein sequence ID" value="CCZ83986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01715"/>
    <dbReference type="EnsemblBacteria" id="CCZ83986">
        <property type="protein sequence ID" value="CCZ83986"/>
        <property type="gene ID" value="BN566_01715"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027275">
        <property type="entry name" value="PRC-brl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014238">
        <property type="entry name" value="Spore_YlmC/YmxH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40061">
        <property type="entry name" value="PTHR40061"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05239">
        <property type="entry name" value="PRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="SSF50346"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02888">
        <property type="entry name" value="spore_YlmC_YmxH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRC" evidence="1">
        <location>
            <begin position="6"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="88" mass="10040" checksum="342E3B28277D2AFB" modified="2013-07-24" version="1">MLCCIEELREKDVINVCDGENLGYVDDVRVDTQTHTIEALILYGKPRFFGWFGARETIAIPFTRVQLVGKDVILVCIPHENCTDILKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5VKJ4</accession>
    <name>R5VKJ4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01735</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000178">
        <property type="protein sequence ID" value="CCZ84006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01735"/>
    <dbReference type="EnsemblBacteria" id="CCZ84006">
        <property type="protein sequence ID" value="CCZ84006"/>
        <property type="gene ID" value="BN566_01735"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="261" mass="28146" checksum="FFA30B874310673C" modified="2013-07-24" version="1">MSKFHPMPDACPDAVPIKVDRVFDSCSDRDCLSNVQILMDGGELPPNVTLVKSRCVKVSDICMNIEPVPFNRGFFSIDLTYTFRVELMAYERACGSPTLLTGTAYAGKNVILYGGESSSKTFFSNGKTCSSEEACSESVNLPTAAVQVVEPIALETRVGMVCPPAPEGSSAVPIRTVIMTLGLFSVVELFRPVTILVPTYEYTIPTKECRADTESPCAVFDKIRFPAEEFSPGSVNGMGDFSPDCGCGATTDSIESEEKPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>R5VKS4</accession>
    <name>R5VKS4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">7-cyano-7-deazaguanine synthase</fullName>
            <ecNumber evidence="1">6.3.4.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">7-cyano-7-carbaguanine synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">PreQ(0) synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Queuosine biosynthesis protein QueC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">queC</name>
        <name evidence="2" type="ORF">BN566_01847</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP-dependent conversion of 7-carboxy-7-deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0)).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>7-carboxy-7-deazaguanine + ATP + NH4(+) = 7-cyano-7-deazaguanine + ADP + H(+) + H2O + phosphate</text>
            <dbReference type="Rhea" id="RHEA:27982"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:45075"/>
            <dbReference type="ChEBI" id="CHEBI:61036"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the QueC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.20" evidence="1"/>
    <dbReference type="EMBL" id="CBAR010000187">
        <property type="protein sequence ID" value="CCZ84121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01847"/>
    <dbReference type="EnsemblBacteria" id="CCZ84121">
        <property type="protein sequence ID" value="CCZ84121"/>
        <property type="gene ID" value="BN566_01847"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00391"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008616">
        <property type="term" value="P:queuosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01995">
        <property type="entry name" value="ExsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01633">
        <property type="entry name" value="QueC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018317">
        <property type="entry name" value="QueC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42914">
        <property type="entry name" value="PTHR42914"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06508">
        <property type="entry name" value="QueC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006293">
        <property type="entry name" value="ExsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00364">
        <property type="entry name" value="TIGR00364"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0671">Queuosine biosynthesis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="226" mass="24581" checksum="29DE1C019C1BD9F9" modified="2013-07-24" version="1">MKAMVLFSGGVDSTTALALAVEKYGASEVLALSIYYGQKHKKELEAAQKVVNYYGVAWKQLDLTTIFADSNCSLLENSTEEIPQESYAEQLEKTNGAPVSTYVPFRNGLFLASAASMALSNGCEVIYYGPHADDAAGNAYPDCSTVFNDAMKTAIYEGSGHELRVEAPFVTWTKKDIVKKGLELHVPYELTWSCYEGGEKPCGVCGTCRDRQAAFLANGVEDPLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>R5VKV3</accession>
    <name>R5VKV3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">AraC-type DNA-binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01881</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000193">
        <property type="protein sequence ID" value="CCZ84156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84156">
        <property type="protein sequence ID" value="CCZ84156"/>
        <property type="gene ID" value="BN566_01881"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="1">
        <location>
            <begin position="139"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="249" mass="29525" checksum="2F60AC5441AC26E2" modified="2013-07-24" version="1">MNIEKELTYVAFLNREYQRSHLAYDREMEFFRAIQQGDIEMAHRLFKPLGSEKMGALSENPLRNLIYHFVITVAFLTRYCIEGGMEMEEAFNLSDIYIRSADRCQKEQEISVLHRQVIDDFAQRMQIIHKKTIYSKPILVALDYIYDHLHTKITLQALAEEVHLSPSHLSKLFHREMGLTISDYIRKKRVEAAGNMLKFSDYSCAEIANYLCFSSESHFIQIFKKEMGKTPKQYRELFFRRNWNTQTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5VKW3</accession>
    <name>R5VKW3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">CrtK protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_01891</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TspO/BZRP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000193">
        <property type="protein sequence ID" value="CCZ84166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01891"/>
    <dbReference type="EnsemblBacteria" id="CCZ84166">
        <property type="protein sequence ID" value="CCZ84166"/>
        <property type="gene ID" value="BN566_01891"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15904">
        <property type="entry name" value="TSPO_MBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038330">
        <property type="entry name" value="TspO/MBR-related_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004307">
        <property type="entry name" value="TspO_MBR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10057">
        <property type="entry name" value="PTHR10057"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03073">
        <property type="entry name" value="TspO_MBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005859">
        <property type="entry name" value="PBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="9"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="138"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ84166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="159" mass="17972" checksum="78CC2F05E6DBD09E" modified="2013-07-24" version="1">MKKGAWIDFSLWLAATELVGILSGWLAGDIRAVYQNLHQPPFSPPGWVFPVMWGILYAMMAISAYLIQHADSTLWKKRRSALILYAVQLAINFSWSIVFFRFQKFGAAIGVIGLLILLVIAMIVIFRKIRPAAAGWNIPYLLWLLFAAYLNIGTFLLNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>R5VL62</accession>
    <name>R5VL62_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC transporter permease/ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN566_00025</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000208">
        <property type="protein sequence ID" value="CCZ84286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00025"/>
    <dbReference type="EnsemblBacteria" id="CCZ84286">
        <property type="protein sequence ID" value="CCZ84286"/>
        <property type="gene ID" value="BN566_00025"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="PTHR24221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="32"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="74"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="159"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="274"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="35"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="391"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="424"/>
            <end position="431"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ84286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="631" mass="69441" checksum="268C9939DE3AD46F" modified="2013-07-24" version="1">MSKFKTASPTGTAENPMVVLKRLLRYLKPYRLRLAVVFLCIIFSALASTISATFLKTLINDYIAPLLLESHADYSGLLSMILKMAVIFAGGAICTLLYIRLMVPVSQGVLKKIRDDMFTHMQKLPVRYFDQNATGDIMSLYTNDTDTLRQMMAQSLPQFISSTITIVAVFVAMLVTSVWLTLFVIAFLIFMMVIVKHVGGKSGKYFVAQQASLGDVNGYVEEMIHGQKVVKVFCHEPQAAAVFDEKNEGLFENATKANSFANILMPITNNLGNLQYVCIAILGGVLALHGVGGLTLGAIAAFLQLSKSFTQPIGQISQQASAIVMALAGAGRIFHMLDEQPEQDEGYVHLVNAERDADGNLRKADHRTHLWAWEHPHQDGTVTYTQLQGDVQMEHVDFGYVPEKIVLHDVSLYAKPGQKIAFVGATGAGKTTITNLINRFYDIADGKVRYDNININKICKADLRRSMGMVLQDVNLFTGTVMDNIRYGRLDATDEECIAAAKRANAHDFIMLLPDGYETVLEGDGSGLSQGQRQLLSIARAAVADPPVMILDEATSSIDTRTERIVQAGMDALMKGRTVFVIAHRLSTIQNADVILVLDHGCVIERGNHAQLMAQKGRYYQLQTGTLPPLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5VL88</accession>
    <name>R5VL88_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase_C39_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00224</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000212">
        <property type="protein sequence ID" value="CCZ84311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84311">
        <property type="protein sequence ID" value="CCZ84311"/>
        <property type="gene ID" value="BN566_00224"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039564">
        <property type="entry name" value="Peptidase_C39-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13529">
        <property type="entry name" value="Peptidase_C39_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_C39_2" evidence="2">
        <location>
            <begin position="116"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="317" mass="35186" checksum="C9CBEB165BF79E2D" modified="2013-07-24" version="1">MAESATPHAPRPKKRVTRTQARNRRIIALLLLALFVFLIRKGCVACVHSHNDKKNNTAAVTTTTTEETTTVPEPASILPETTAPEPTPTIPQTTATDPPTSEYLISGDLPTTYSMEVDPILQLPELPTGCEITTLAMLLDAVGFQVDKVQLAEGYLTCKSEGEATFQQAFIGSPYDSAGYGCYAPVIVDTARKYLAAQNSGRIVKDLTGAKFEDLLREVASNHPVAIWASIDLVDIQEVYAYTIYNYTETNSDGSTSTPKNLDVYWLENEHVYLLKGYDLDRNVVIVNDSLNGEMEYDMNRFKECYEQCYKQAVIIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>R5VLJ5</accession>
    <name>R5VLJ5_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ATP synthase subunit a</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">ATP synthase F0 sector subunit a</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">F-ATPase subunit 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">atpB</name>
        <name evidence="12" type="ORF">BN566_00323</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Key component of the proton channel; it plays a direct role in the translocation of protons across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell membrane</location>
            <topology evidence="11">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the ATPase A chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000225">
        <property type="protein sequence ID" value="CCZ84416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00323"/>
    <dbReference type="EnsemblBacteria" id="CCZ84416">
        <property type="protein sequence ID" value="CCZ84416"/>
        <property type="gene ID" value="BN566_00323"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045263">
        <property type="term" value="C:proton-transporting ATP synthase complex, coupling factor F(o)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01393">
        <property type="entry name" value="ATP_synth_a_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045082">
        <property type="entry name" value="ATP_syn_F0_a_bact/chloroplast"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000568">
        <property type="entry name" value="ATP_synth_F0_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035908">
        <property type="entry name" value="F0_ATP_A_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42823">
        <property type="entry name" value="PTHR42823"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00119">
        <property type="entry name" value="ATP-synt_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00123">
        <property type="entry name" value="ATPASEA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81336">
        <property type="entry name" value="SSF81336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 11" id="KW-0138">CF(0)</keyword>
    <keyword evidence="6 11" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="8 11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="83"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="172"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="204"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CCZ84416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="231" mass="25390" checksum="CB2743E6F37F625F" modified="2013-07-24" version="1">MGEALAEELQTEVAFTIPLFGGIPVHESIVVMWGIMAFIMIVVLLLTRNLKIVPGRGQAMLESAVTWVNNFLEGNMGKAGRPYFAYLGTVLLLLGISNLIGMFGFGIKPPTKDINVTVAFALLSIILIEVSTFRAQGGLKGFLSSFTKPMAIMTPINIMEIGIRPLSLCMRLFGNVLGAFVVMKLIEHVCGLIVPMVFSMYFDVFDGLIQAYVFVFLTSLFMAEGMEHHEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>R5VLS2</accession>
    <name>R5VLS2_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">3-oxoacyl-[acyl-carrier-protein] synthase 3</fullName>
            <ecNumber evidence="8">2.3.1.180</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">3-oxoacyl-[acyl-carrier-protein] synthase III</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Beta-ketoacyl-ACP synthase III</fullName>
            <shortName evidence="8">KAS III</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">fabH</name>
        <name evidence="11" type="ORF">BN566_00385</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Catalyzes the first condensation reaction which initiates fatty acid synthesis and may therefore play a role in governing the total rate of fatty acid production. Possesses both acetoacetyl-ACP synthase and acetyl transacylase activities. Its substrate specificity determines the biosynthesis of branched-chain and/or straight-chain of fatty acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>acetyl-CoA + H(+) + malonyl-[ACP] = 3-oxobutanoyl-[ACP] + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:12080"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9625"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78450"/>
            <dbReference type="EC" id="2.3.1.180"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">The last Arg residue of the ACP-binding site is essential for the weak association between ACP/AcpP and FabH.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the thiolase-like superfamily. FabH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.180" evidence="8"/>
    <dbReference type="EMBL" id="CBAR010000240">
        <property type="protein sequence ID" value="CCZ84486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00385"/>
    <dbReference type="EnsemblBacteria" id="CCZ84486">
        <property type="protein sequence ID" value="CCZ84486"/>
        <property type="gene ID" value="BN566_00385"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033818">
        <property type="term" value="F:beta-ketoacyl-acyl-carrier-protein synthase III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01815">
        <property type="entry name" value="FabH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013751">
        <property type="entry name" value="ACP_syn_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013747">
        <property type="entry name" value="ACP_syn_III_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004655">
        <property type="entry name" value="FabH_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08545">
        <property type="entry name" value="ACP_syn_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08541">
        <property type="entry name" value="ACP_syn_III_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00747">
        <property type="entry name" value="fabH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="4 8" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 8" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5 8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ACP_syn_III" evidence="10">
        <location>
            <begin position="105"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="ACP_syn_III_C" evidence="9">
        <location>
            <begin position="248"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="ACP-binding" evidence="8">
        <location>
            <begin position="264"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="111"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="263"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ84486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="336" mass="36585" checksum="9E7B3DD0D65A59FB" modified="2013-07-24" version="1">MGIHILGTGSYTPPCTLTNADLSEMVETNDEWIRTRTGISVRHVSDGEPTWYMGVQAAQQAIATAGIDPADIGLIIDTTITGEFCTPSMSCIIQRETKAVNAACFDLNAACSGFVYAMDTAHRFLMTDPHMKYALVVANESLSKITNYSDRSSCILFGDGAAAAIIEYAPDALYTSHLGADGTGAKYLYAHSALPRSPFVKAKREVECGDPDMQLAPQGWEYTMLQDGREVYRFATHALEKAARLAAEKIGFDLNDLDKVVPHQANMRIIETAMKYLKQPMEKVIVNIEHHGNTSSASIPIALDEGIREGKIQRGDKVCLVGFGAGLTYAAIIMEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5VLW6</accession>
    <name>R5VLW6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00400</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000240">
        <property type="protein sequence ID" value="CCZ84501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00400"/>
    <dbReference type="EnsemblBacteria" id="CCZ84501">
        <property type="protein sequence ID" value="CCZ84501"/>
        <property type="gene ID" value="BN566_00400"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007060">
        <property type="entry name" value="FtsL/DivIC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04977">
        <property type="entry name" value="DivIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="59"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="127" mass="14722" checksum="A93D33A6792BEDA1" modified="2013-07-24" version="1">MAGAGKRNRLQQRKKKQIFNDAVQYNQPEAVKNKKGKFLFKLLIRITIVLALIGCTASIISLQSNIAERKTELDTLRSQAETYEAENEDLERILNSGDIDSYMEKLAREDYGYAYPDEYRFYDTSRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5VM38</accession>
    <name>R5VM38_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Citrate synthase (unknown stereospecificity)</fullName>
            <ecNumber evidence="2">2.3.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00095</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Carbohydrate metabolism; tricarboxylic acid cycle.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.16" evidence="2"/>
    <dbReference type="EMBL" id="CBAR010000252">
        <property type="protein sequence ID" value="CCZ84601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00095"/>
    <dbReference type="EnsemblBacteria" id="CCZ84601">
        <property type="protein sequence ID" value="CCZ84601"/>
        <property type="gene ID" value="BN566_00095"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00223"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036440">
        <property type="term" value="F:citrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016142">
        <property type="entry name" value="Citrate_synth-like_lrg_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016143">
        <property type="entry name" value="Citrate_synth-like_sm_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002020">
        <property type="entry name" value="Citrate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036969">
        <property type="entry name" value="Citrate_synthase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11739">
        <property type="entry name" value="PTHR11739"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00285">
        <property type="entry name" value="Citrate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00143">
        <property type="entry name" value="CITRTSNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48256">
        <property type="entry name" value="SSF48256"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="462" mass="51880" checksum="D9251F9B10C33149" modified="2013-07-24" version="1">MTMQENYLTEETDIPQSDITRLCDNLRKHSQIAPALYEKYNVNRGLRHSDGSGVVAGITRICSVHGYVIDEGERRPVPGELIYRGYSIKDLVENAEREDRFLFEEIVYLLLMGELPTADELDVMRKSLAMRRELPPGFLVDSIMQAPSKNIMNKLARSILSLYSYDDDPEHPSLEGEMRIALGLIAKMPILMDGAYQAKRHKFDNASMIIHQIRPEEGTAESILSLIRNDRNYTKEEAQLLDIMLSIHAEHGGGNNSTFACRVATSSGTDPYAAYTTAINSLKGSRHGGANIKVVQMLDCIKENVKNWSNRGEVADFITKILKKEAGDGSGLIYGMGHAVYTLSDPRAVILKRKAFEMAKGKEIEAEFQLLSLIEELTPILMKEVRGVDKAMCANVDMYSGLVYRMLGIPDELFTPLFASARMVGWAAHRMEELLTGKRIIRPAYKPITKVKPYIPLCDRKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5VQG7</accession>
    <name>R5VQG7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">50S ribosomal protein L32</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpmF</name>
        <name evidence="5" type="ORF">BN566_00637</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the bacterial ribosomal protein bL32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000012">
        <property type="protein sequence ID" value="CCZ82832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00637"/>
    <dbReference type="EnsemblBacteria" id="CCZ82832">
        <property type="protein sequence ID" value="CCZ82832"/>
        <property type="gene ID" value="BN566_00637"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00340">
        <property type="entry name" value="Ribosomal_L32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002677">
        <property type="entry name" value="Ribosomal_L32p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044957">
        <property type="entry name" value="Ribosomal_L32p_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35534">
        <property type="entry name" value="PTHR35534"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01783">
        <property type="entry name" value="Ribosomal_L32p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="SSF57829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01031">
        <property type="entry name" value="rpmF_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ82832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="61" mass="6840" checksum="18AFA60B4C05869E" modified="2013-07-24" version="1">MAVPKRKVSKARRDKRRSAVWKLDAPAMTTCDHCGAYKAPHKVCKNCGYYKGVAVLKMDAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5VQK5</accession>
    <name>R5VQK5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Intein C-terminal splicing region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00098</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000013">
        <property type="protein sequence ID" value="CCZ82862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00098"/>
    <dbReference type="EnsemblBacteria" id="CCZ82862">
        <property type="protein sequence ID" value="CCZ82862"/>
        <property type="gene ID" value="BN566_00098"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029501">
        <property type="entry name" value="EndoU_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036844">
        <property type="entry name" value="Hint_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030934">
        <property type="entry name" value="Intein_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14436">
        <property type="entry name" value="EndoU_bacteria"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51294">
        <property type="entry name" value="SSF51294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50818">
        <property type="entry name" value="INTEIN_C_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="INTEIN_C_TER" evidence="2">
        <location>
            <begin position="30"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ82862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="177" mass="19221" checksum="E6835404F78C8E55" modified="2013-07-24" version="1">MLVGDKLVSVNGEDLIIEDYYLELTEEPVSVYNFQVEDFHTYFVGDCAVWVHNAECKVSSSRREHILEGEGPNDPGHGPNRGFGNSAFPDTWTDDYAIKAVENVANSPNSTWQQSTGPGGGRNAPVTIGGPDVNAPLTTRNGRPVRFVVEGRNHGLDVRVIVEPGGEGIVTGFPINR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VRD2</accession>
    <name>R5VRD2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00904</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000059">
        <property type="protein sequence ID" value="CCZ83117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83117">
        <property type="protein sequence ID" value="CCZ83117"/>
        <property type="gene ID" value="BN566_00904"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="52" mass="5845" checksum="27674F7DA7E3B0C9" modified="2013-07-24" version="1">MTKEIEIYKVSMAVLRNFLKSGLLTQSEYVQCEQTLAEKCGLSLGSIFRESA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>R5VRV0</accession>
    <name>R5VRV0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Sulfate ABC transporter permease protein CysW</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN566_01068</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">The complex is composed of two ATP-binding proteins (CysA), two transmembrane proteins (CysT and CysW) and a solute-binding protein (CysP).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01068"/>
    <dbReference type="EnsemblBacteria" id="CCZ83287">
        <property type="protein sequence ID" value="CCZ83287"/>
        <property type="gene ID" value="BN566_01068"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015419">
        <property type="term" value="F:ABC-type sulfate transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011866">
        <property type="entry name" value="CysW_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005667">
        <property type="entry name" value="Sulph_transpt2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30406">
        <property type="entry name" value="PTHR30406"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30406:SF1">
        <property type="entry name" value="PTHR30406:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00969">
        <property type="entry name" value="3a0106s02"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02140">
        <property type="entry name" value="permease_CysW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0764">Sulfate transport</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="56"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="94"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="131"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="195"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="241"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="59"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ83287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="273" mass="30115" checksum="138316AB0292B9C6" modified="2013-07-24" version="1">MHEETVGSKVVKWLLIGISALFLIVMLLLPLITVISEALRSGWETYLKAVTDRYTIKAILLTLEATICAVLINTIFGVFAAWAVTKFHFKGKKLLTTLIDVPVTISPVIAGLIFVLTFGRQSFLYPYLQQLGIQVVFAVPGIVLATIFVTFPFISRELIPVLESQGTDEEEAAALMGAKGLTIFRRVTFPHIKWAFLYGVVLCAARAMGEFGAVSVLSGHLRGKTNTLPLHVEILFNEFQYVPAFAVSSILVLMAIVILIIRSVIEYRGKKVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>R5VS20</accession>
    <name>R5VS20_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BN566_01123</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the helicase family. UvrD subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3"/>
    <dbReference type="EMBL" id="CBAR010000092">
        <property type="protein sequence ID" value="CCZ83347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01123"/>
    <dbReference type="EnsemblBacteria" id="CCZ83347">
        <property type="protein sequence ID" value="CCZ83347"/>
        <property type="gene ID" value="BN566_01123"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013986">
        <property type="entry name" value="DExx_box_DNA_helicase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014017">
        <property type="entry name" value="DNA_helicase_UvrD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000212">
        <property type="entry name" value="DNA_helicase_UvrD/REP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038726">
        <property type="entry name" value="PDDEXK_AddAB-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014016">
        <property type="entry name" value="UvrD-like_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070">
        <property type="entry name" value="PTHR11070"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12705">
        <property type="entry name" value="PDDEXK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00580">
        <property type="entry name" value="UvrD-helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13361">
        <property type="entry name" value="UvrD_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="SSF52980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51198">
        <property type="entry name" value="UVRD_HELICASE_ATP_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="10 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UvrD-like helicase ATP-binding" evidence="11">
        <location>
            <begin position="5"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="26"/>
            <end position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CCZ83347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="958" mass="111228" checksum="EA77DA6266A88A7A" modified="2013-07-24" version="1">MFDFGNANDAQKLAISSSNGAVLITAGPGTGKTFTLVQRAIYLIQECGVKPEQILMATFTEKAAKELVTRITNELAVRNIYVNVNEMYIGTFHSLCLRIIKDNLEFTQLKKNYRLLDSFDQKYLVFRNIQKFRNIADIELVMPNGGAWKWAEAICGLVGTLTEELVDADAMAKDSKTEIRVLSAILKQYQQLLTDENLIDFAKIQTECYRLLKENPAILSDLQEKIHYLMIDEYQDTNYIQEQIIFMLGEKHGNICVVGDDDQGLYRFRGATIRNILEFPHKFKDGACKIIPLTVNYRSNSDIVDFYNEWISTTDGAKFKFEWKNFRYPKHIVPFKHTDMNSKAVVKLASSEDEDEWHDKILDFIHKLKDSGKLTDYNQLAFLFSSVKSQQVTSLANFLEANGINVYSPRSDMFFKRREIMLTIGCLMLMFPLYVQGLIKGEHKYLQTEHSYYYRDCIELANELLSLEENKELKKFIRSRGKSHAALAQSKGTTDYAYSGLLYQLFAFKPFSTILDTDLSSGVVDVRPARNIAMLTQIVGKFEYLHRIDVFTDKYINKNTELFFNLYMRLLFDGGISEYEDDSEYAPSGCVSFMTIHQSKGMEFPIVIVDSLGNTPRKSYKDLLSEVEVKYYHRPQFEPFEEMKFFDFWRLYYTAFSRAQDLLVLTCNENNRTPSLYFRELYDELPSIESSAFDLNEFTFHKVKAVNIKDTFSFTSHITVYETCSLQYKFYKELEFMPIRENAMMFGTLVHETIEDIHRAAIRQEEHLITPDNVSDWFESNYVSLVKSEHTYLAEPQKKAALKQVLRYVERQHGDWSMIKQAEVDVSLVQPEYIIEGKIDLIKGADGTVELVDFKSERKPDMVKARDRIEHYRRQLHIYAFLVEQRTGQKVSKMHLYYTGEENGNPMISFPYTKSAIEGTVSAFDDTVHKIMKKDFSRQAESLKTCTGCDFRFYCQNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5VSV5</accession>
    <name>R5VSV5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dipicolinic acid synthetase B subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00144</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000146">
        <property type="protein sequence ID" value="CCZ83617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00144"/>
    <dbReference type="EnsemblBacteria" id="CCZ83617">
        <property type="protein sequence ID" value="CCZ83617"/>
        <property type="gene ID" value="BN566_00144"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014214">
        <property type="entry name" value="Dipicolinic_acid_synth_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001390">
        <property type="entry name" value="Dipicolinate_synth_subunit_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="SSF52507"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02852">
        <property type="entry name" value="spore_dpaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavoprotein" evidence="1">
        <location>
            <begin position="7"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="196" mass="21047" checksum="D520E5401FE053C1" modified="2013-07-24" version="1">MKETIGKRIGFAVTGSFCTFSAAFAEAERLVAAGYSLTPIFSEHAASIDTRFGKAVEQRKKLEQICGKPALLTISDVEPLGPKDRLDALLVAPCTANTMAKLAMGITDTTVTMAVKSMLRNEKPIILALATNDALRASAKNLGLLLNTKNYYFVPLRQDAPVKKPSSLVADFTQISDTVAVALGGLQIQPMFIQNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>R5VT46</accession>
    <name>R5VT46_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphoribosylaminoimidazole-succinocarboxamide synthase</fullName>
            <ecNumber evidence="8">6.3.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">SAICAR synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">purC</name>
        <name evidence="10" type="ORF">BN566_01443</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + ATP + L-aspartate = (2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate + ADP + 2 H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:22628"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58443"/>
            <dbReference type="ChEBI" id="CHEBI:77657"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the SAICAR synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.6" evidence="8"/>
    <dbReference type="EMBL" id="CBAR010000154">
        <property type="protein sequence ID" value="CCZ83707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01443"/>
    <dbReference type="EnsemblBacteria" id="CCZ83707">
        <property type="protein sequence ID" value="CCZ83707"/>
        <property type="gene ID" value="BN566_01443"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00131"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004639">
        <property type="term" value="F:phosphoribosylaminoimidazolesuccinocarboxamide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01415">
        <property type="entry name" value="SAICAR_synt_PurC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00137">
        <property type="entry name" value="SAICAR_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028923">
        <property type="entry name" value="SAICAR_synt/ADE2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033934">
        <property type="entry name" value="SAICAR_synt_PurC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001636">
        <property type="entry name" value="SAICAR_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018236">
        <property type="entry name" value="SAICAR_synthetase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01259">
        <property type="entry name" value="SAICAR_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00081">
        <property type="entry name" value="purC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01057">
        <property type="entry name" value="SAICAR_SYNTHETASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01058">
        <property type="entry name" value="SAICAR_SYNTHETASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAICAR_synt" evidence="9">
        <location>
            <begin position="10"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ83707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="239" mass="27039" checksum="59108FE87DDA9644" modified="2013-07-24" version="1">MENLQKKEQMYEGKAKKVYATNQEGIVIVDYKDDATAFNGEKKGTIVGKGVINNKMSNYIMKQLEAAGVPTHLVEELSDRETAVKKVSIVPLEVIVRNVAAGSFSKRMGVEEGKALLRPIIEFSYKCDELNDPFINDDYALALGLATEEEIKTIKAYTAKVNEVLKEFFLKIGIRLIDFKIEFGRLADGTIILADEISPDTCRLWDVKTNEKLDKDRFRRDMGGTEEAYQEVMHRIFGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5VTP3</accession>
    <name>R5VTP3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01621</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000170">
        <property type="protein sequence ID" value="CCZ83892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01621"/>
    <dbReference type="EnsemblBacteria" id="CCZ83892">
        <property type="protein sequence ID" value="CCZ83892"/>
        <property type="gene ID" value="BN566_01621"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003735">
        <property type="entry name" value="Metal_Tscrpt_repr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038390">
        <property type="entry name" value="Metal_Tscrpt_repr_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33677">
        <property type="entry name" value="PTHR33677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02583">
        <property type="entry name" value="Trns_repr_metal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="104" mass="12029" checksum="BDA661CBBF4BC60E" modified="2013-07-24" version="1">MEEQEEKTCPCCHKKKVRSEEEYTKLIHRLNRIEGQIRGIRGMVEKSAYCPDILVQVAAVNAALHAFNRELLSQHIRTCVAQDIREGHDEVIEELVTTLQKLMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5VUF1</accession>
    <name>R5VUF1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Bacterial membrane flanked domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01872</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000192">
        <property type="protein sequence ID" value="CCZ84147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84147">
        <property type="protein sequence ID" value="CCZ84147"/>
        <property type="gene ID" value="BN566_01872"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005182">
        <property type="entry name" value="DUF304"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03703">
        <property type="entry name" value="bPH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="bPH_2" evidence="2">
        <location>
            <begin position="68"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="159" mass="18166" checksum="46C2DEF87A50A7FD" modified="2013-07-24" version="1">MNHLQKSYRPDPHAQYLLQLLALPTALVLIAAVRYVLKVVPAGIVNLICLLLLLAALLGIFILLPFWFRNTRYIITEQDIISISGIFIRREKRMRLRALQCSTIVQTPFCKYTGMTFLPLHAYGSTLILLFLKKEDAAELCMRFADLFYLAAQEESHAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5VUI2</accession>
    <name>R5VUI2_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Isochorismatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01912</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01912"/>
    <dbReference type="EnsemblBacteria" id="CCZ84187">
        <property type="protein sequence ID" value="CCZ84187"/>
        <property type="gene ID" value="BN566_01912"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.850">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000868">
        <property type="entry name" value="Isochorismatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00857">
        <property type="entry name" value="Isochorismatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52499">
        <property type="entry name" value="SSF52499"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isochorismatase" evidence="1">
        <location>
            <begin position="4"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="179" mass="19662" checksum="686EA19C03FA66D9" modified="2013-07-24" version="1">MKRVLIVVDYQRDFVTGSLGFPGAVALAERIAKKIAAYHKSGDVVLFTMDTHTDDYRNTQEGKHLPVPHCIKGTAGHTLVPEVAEQVEPQDLFFEKDTFGSTDLFVYLRTQQYASIELVGVVSSICVLSNAVLCKTALPEVPILVDSSCIAAPDPTLQEAAILMMKNLQMQILPEEETP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5VV07</accession>
    <name>R5VV07_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00265</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000218">
        <property type="protein sequence ID" value="CCZ84352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00265"/>
    <dbReference type="EnsemblBacteria" id="CCZ84352">
        <property type="protein sequence ID" value="CCZ84352"/>
        <property type="gene ID" value="BN566_00265"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043571">
        <property type="term" value="P:maintenance of CRISPR repeat elements"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006482">
        <property type="entry name" value="Cas7_Csh2/Csh2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013418">
        <property type="entry name" value="CRISPR-assoc_prot_Csd2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05107">
        <property type="entry name" value="Cas_Cas7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02589">
        <property type="entry name" value="cas_Csd2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01595">
        <property type="entry name" value="cas_CT1132"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="283" mass="31638" checksum="02E8AD9532342E24" modified="2013-07-24" version="1">MSTLSKKIDFAILISVENANPNGDPLCGNRPREDYDGYGEISDVCLKRKIRNRLIDMNESVFVQSNDHRVDSYKSLKTRAEGCEELKKASNGKDTDQDEFARIACEQWMDVRSFGQVFAFKGDKVSVGVRGPVTISIAKSVSPVDIISMQIVKSVNSEDKTERASDTMGMKHRVAFGLYLAKGSINCQLAEKTGFRDEDAEKIKEAMRTLLENDASSARPEGSMEVCALYWWEQEGKTPKYSSAKVQRSLQVTLKEGVIKPKRFEDYDVQVAPLDGVTLQILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVN9</accession>
    <name>R5VVN9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Histidinol-phosphate transaminase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00433</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000247">
        <property type="protein sequence ID" value="CCZ84572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00433"/>
    <dbReference type="EnsemblBacteria" id="CCZ84572">
        <property type="protein sequence ID" value="CCZ84572"/>
        <property type="gene ID" value="BN566_00433"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-bd_dom" evidence="1">
        <location>
            <begin position="6"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="320" mass="35255" checksum="B10538FBC729DDF9" modified="2013-07-24" version="1">MKAMLIGGAGFVGKHLAIHLANTYGWEIVITGLPEEREAIERSSLGRFIPVNILEKGQIQAVLEQERPHYIFHLAAQSSVARSWKNPQATIDVNIKGCANLLDAIRSIEIYDPKILLIGSGDEYGRLPDGIRVVSETTLPCPGNPYAMTKAAQTMLGQVYAHAYRMHIVLARPFNHIGPGQMAGFVVSDFCKQVAEILNGAEPVVRVGNLTASRDFTDVRDVVRAYGLLIQHGVPGEIYNIGSGTAVSIQSLLDEIIRQSGISISVQHDPQKFRPVDYPSIAADISKLQRAIDWKPEISLERTIADTLTDWQIRLKNKVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVR8</accession>
    <name>R5VVR8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">Signal peptidase I</fullName>
            <ecNumber evidence="4 7">3.4.21.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN566_00470</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.</text>
            <dbReference type="EC" id="3.4.21.89"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Membrane</location>
            <topology evidence="7">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the peptidase S26 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.89" evidence="4 7"/>
    <dbReference type="EMBL" id="CBAR010000257">
        <property type="protein sequence ID" value="CCZ84617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00470"/>
    <dbReference type="EnsemblBacteria" id="CCZ84617">
        <property type="protein sequence ID" value="CCZ84617"/>
        <property type="gene ID" value="BN566_00470"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000223">
        <property type="entry name" value="Pept_S26A_signal_pept_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019758">
        <property type="entry name" value="Pept_S26A_signal_pept_1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019756">
        <property type="entry name" value="Pept_S26A_signal_pept_1_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43390">
        <property type="entry name" value="PTHR43390"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10502">
        <property type="entry name" value="Peptidase_S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00727">
        <property type="entry name" value="LEADERPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="SSF51306"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02227">
        <property type="entry name" value="sigpep_I_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00501">
        <property type="entry name" value="SPASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00761">
        <property type="entry name" value="SPASE_I_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 7" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="30"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S26" evidence="8">
        <location>
            <begin position="32"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="62"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR600223-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ84617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="193" mass="21522" checksum="D5DD845BDCD8CD2D" modified="2013-07-24" version="1">MEKQTIDFPTAAQLTEELKRIRYRKGFRKMLRSTISSLLVVAAVAVLISMLFLPVLRVTGTSMTPTLMNDELVICGKRSNFKSGDVVAFYFNNKILLKRVIGVAGDVIDIKEDGTVYVNGTALEEPYVNELAFGECDLELPYQVPESRIFVMGDHRATSVDSRSSVVGCIAEEYVVGRVILRVWPLKEIGTIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVX1</accession>
    <name>R5VVX1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Stage IV sporulation protein B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00530</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000267">
        <property type="protein sequence ID" value="CCZ84677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00530"/>
    <dbReference type="EnsemblBacteria" id="CCZ84677">
        <property type="protein sequence ID" value="CCZ84677"/>
        <property type="gene ID" value="BN566_00530"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041489">
        <property type="entry name" value="PDZ_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014219">
        <property type="entry name" value="Pept_S55_SpoIVB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008763">
        <property type="entry name" value="Peptidase_S55"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17820">
        <property type="entry name" value="PDZ_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05580">
        <property type="entry name" value="Peptidase_S55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="SSF50156"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="SSF50494"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02860">
        <property type="entry name" value="spore_IV_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51494">
        <property type="entry name" value="SPOIVB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S55" evidence="1">
        <location>
            <begin position="176"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="405" mass="43122" checksum="084052BC18024FA0" modified="2013-07-24" version="1">MQTWFRRLAAAMLLTVLPVYAAAGYYAWRLPDSYYVRNGSALALSTALHITAESEPEQVKAAFSSTAATEEQYTLCLFGIVPIKTVSVQTITAPSFIPCGQPFGIKLHMDGVMVVGFGNVTSTDGTCCPAEDAGFQKGDMIHAINTVPVNSSEEVQTSIRNAGSEPLSITIQREGKEQTLTVHPVYSTADQQYETGLWVRDSTAGVGTFTFYEPESGAFGGLGHPICDTDTGTCIPISEGEACPVQIQGVQKGSAGKPGMLQGQFLDDAEPFGKLLCNNRCGVFGMLNQNPAPNTEAIPMAFKQEIVPGEATIFCTISGTEPASYTIEIEEIDYNGSDSTQNMVLSVTDPTLLQETGGIVQGMSGSPILQNGKLVGAVTHVFVDDPTKGYGIFCENMVRFGQVGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5UWY3</accession>
    <name>R5UWY3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine-type D-Ala-D-Ala carboxypeptidase</fullName>
            <ecNumber evidence="4">3.4.16.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">BN566_00633</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the peptidase S11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.4" evidence="4"/>
    <dbReference type="EMBL" id="CBAR010000012">
        <property type="protein sequence ID" value="CCZ82828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00633"/>
    <dbReference type="EnsemblBacteria" id="CCZ82828">
        <property type="protein sequence ID" value="CCZ82828"/>
        <property type="gene ID" value="BN566_00633"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009002">
        <property type="term" value="F:serine-type D-Ala-D-Ala carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015956">
        <property type="entry name" value="Peniciliin-bd_prot_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018044">
        <property type="entry name" value="Peptidase_S11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012907">
        <property type="entry name" value="Peptidase_S11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037167">
        <property type="entry name" value="Peptidase_S11_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001967">
        <property type="entry name" value="Peptidase_S11_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07943">
        <property type="entry name" value="PBP5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00768">
        <property type="entry name" value="Peptidase_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00725">
        <property type="entry name" value="DADACBPTASE1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00936">
        <property type="entry name" value="PBP5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69189">
        <property type="entry name" value="SSF69189"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 15" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="9" id="KW-0133">Cell shape</keyword>
    <keyword evidence="11" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="domain" description="PBP5_C" evidence="14">
        <location>
            <begin position="215"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR618044-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CCZ82828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="321" mass="35062" checksum="7E745E7561A9E7F4" modified="2013-07-24" version="1">MGMMAKLMTVLLAAEQLESGAWTLETVLTASDVVNTCEGATVWLTAGETMTVSDLLKAVIIGNANDAAAVLAEGVSGSVPAFVMEMNARAFDLGMHQTIFTNPQGYDDEKQCTTARDLAKLCQRLAEYETLQPYFQTWRDFLRGEATELVNENTFSRTYEGHIGFKASHTEQSGWCLAEGATRNGMTCIAVVLNGAEDQRFADVKQLLKAGFSQYRVMQPLFSDEFLKPLTVKGGVSTAVRFTADDVHGVVVPKAEPDLTAVLQLPSYIEAPVQKGRKIGSVAFYNGDTMLYETALVTTEPVEAMTFSRAFAKITVKMLKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXD2</accession>
    <name>R5UXD2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">CotH protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00758</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000032">
        <property type="protein sequence ID" value="CCZ82958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00758"/>
    <dbReference type="EnsemblBacteria" id="CCZ82958">
        <property type="protein sequence ID" value="CCZ82958"/>
        <property type="gene ID" value="BN566_00758"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044060">
        <property type="entry name" value="Bacterial_rp_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002105">
        <property type="entry name" value="Dockerin_1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026876">
        <property type="entry name" value="Fn3_assoc_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001322">
        <property type="entry name" value="Lamin_tail_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036415">
        <property type="entry name" value="Lamin_tail_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014867">
        <property type="entry name" value="Spore_coat_CotH_CotH2/3/7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08757">
        <property type="entry name" value="CotH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00404">
        <property type="entry name" value="Dockerin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18998">
        <property type="entry name" value="Flg_new_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13287">
        <property type="entry name" value="Fn3_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00932">
        <property type="entry name" value="LTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74853">
        <property type="entry name" value="SSF74853"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51841">
        <property type="entry name" value="LTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004406518" evidence="1">
        <location>
            <begin position="32"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="domain" description="LTD" evidence="3">
        <location>
            <begin position="25"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="2">
        <location>
            <begin position="815"/>
            <end position="875"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ82958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="875" mass="96744" checksum="125DC49FDDC9D2B1" modified="2013-07-24" version="1">MRMASKWMASTLAGLLTVVSIPMNALQQTQAADFSVWINEICTQNKSCLTDSYGVYSDWIELYNSSDSAIDLSGYGLSDDPTKPLQFTFPEGTVIAAGEHRILFASKQASTASELHTGFALSKNGETVTLASPSGAILQKIAVPALGKNVTYGRSPDGSDTFEIMTATPGTANEIVIDTPVFSASSGFYGTDFSLTLTSSKKDTIYYTLDGSDPTTSETAQIYTDAILVQDRTDQANLWSALAEDNNSATSISRGTGYKAPNFNVDKATVVRAAVQNADGVFSDVVSQTYFVTTSNLAQYQDLTVVSLVTNPENLFDPDTGIYVTGNQYLDWKNSDAYNPDKSVWDTDNLTNYFSKGKEWEREAALTVFQDGKAVVDQNIGIRIKGASSRNSAQKNFNLYARSTYGASKISYPLIPDNYAADGTLIDQYDSVSLRAVSEEGRLRDGFSQKLLTDRTNLTKQDMQSCVVFLNGEYWGLYEMTEKLSDDFIKSNYDIPKENVAMIKNGALEEGSQTELNNFYQFFKKYAAADLTNEDNYQAVCDFVDIDSMIEHYAAGIYLGTYDWPNYNYGVWRNTGDTIAGNPYSDGKWRFISFDYDYTMGKTYKDFGGVEGYAYDNFQHVANANGFPTNLFLNLLHNEDFRNKFVNVYCDYANEVLTPEKADAMIATYKQDYTEQLANTTVRWWGFFGGTKENNLRYQKNTYLNTTLPQIQTFFQKRADYTIEDMCSYLNLSPSMQTITLKTNGNGSIQINSIVPDAATGWSGQYSPDCPITLTAIPAKGAEFLGWGGDLSSTETTVTLTLKEAMSITANFSGAQAVLGDVNADGVCSVADAVLLQKWLLRAGEITDWQAGDFDENQVLDIVDLALLKRKLLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-09-29" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXG5</accession>
    <name>R5UXG5_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RHH_1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00781</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000036">
        <property type="protein sequence ID" value="CCZ82988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82988">
        <property type="protein sequence ID" value="CCZ82988"/>
        <property type="gene ID" value="BN566_00781"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013321">
        <property type="entry name" value="Arc_rbn_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002145">
        <property type="entry name" value="CopG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01402">
        <property type="entry name" value="RHH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="SSF47598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RHH_1" evidence="1">
        <location>
            <begin position="20"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="57" mass="6623" checksum="C66A3B54E3F14574" modified="2013-07-24" version="1">MGIKKGTKLTDNPKEYILRVRMDKTTLAKLDAICKEQGVTRSQVIRNWIVEQYQGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXK4</accession>
    <name>R5UXK4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8 11">Phosphoglycerate kinase</fullName>
            <ecNumber evidence="2 8 11">2.7.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">pgk</name>
        <name evidence="13" type="ORF">BN566_00801</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8 11">
            <text>(2R)-3-phosphoglycerate + ATP = (2R)-3-phospho-glyceroyl phosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:14801"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8 12">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 11">Belongs to the phosphoglycerate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.3" evidence="2 8 11"/>
    <dbReference type="EMBL" id="CBAR010000038">
        <property type="protein sequence ID" value="CCZ83008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00801"/>
    <dbReference type="EnsemblBacteria" id="CCZ83008">
        <property type="protein sequence ID" value="CCZ83008"/>
        <property type="gene ID" value="BN566_00801"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00185"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004618">
        <property type="term" value="F:phosphoglycerate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00318">
        <property type="entry name" value="Phosphoglycerate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1260">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00145">
        <property type="entry name" value="Phosphoglyc_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001576">
        <property type="entry name" value="Phosphoglycerate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015911">
        <property type="entry name" value="Phosphoglycerate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015824">
        <property type="entry name" value="Phosphoglycerate_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036043">
        <property type="entry name" value="Phosphoglycerate_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406">
        <property type="entry name" value="PTHR11406"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00162">
        <property type="entry name" value="PGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000724">
        <property type="entry name" value="Pgk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00477">
        <property type="entry name" value="PHGLYCKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53748">
        <property type="entry name" value="SSF53748"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00111">
        <property type="entry name" value="PGLYCERATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8 12" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="5 8 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8 11" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="8 9">
        <location>
            <begin position="24"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8 9">
        <location>
            <begin position="62"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8 9">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 9">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 9">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="335"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <begin position="361"/>
            <end position="364"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000724-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000724-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CCZ83008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="405" mass="42824" checksum="EB0C10AE3C149B3D" modified="2013-07-24" version="1">MAGLNKVTVDDIDVKGKKVLVRVDFNVPMKDGVITNDNRIQAALPTIKKLVSEGGKVILCSHLGKPKNGPEAKFSLKAAAERLAELVAPTKVTFVPSDKVVDDSVKAAVDAMQDGEIIVLENTRFRGAEETKNGEEFSKELASLADVFVMDAFGSAHRAHASTAGVTKFVKTTAVGYLMQKEIQFLGNAVEDPVRPFVAILGGAKVADKLNVISNLLEKCDTLIIGGGMAYTFLKAEGYEVGTSLVDDTKLDYCKEMIEKAKANGKQLLLPVDTVTAKAFPDPIDAAIEVETYDAQHLPADRMGLDIGEKTQKLFADAVKSAKTVVWNGPMGVFENPVLAKGTIAVAQALADTDATTIIGGGDSAAAVMQLGFADKMSHISTGGGASLEYLEGKELPGVAVIANK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXL1</accession>
    <name>R5UXL1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Probable transcriptional regulatory protein RUM_15350</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00811</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TACO1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000039">
        <property type="protein sequence ID" value="CCZ83018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00811"/>
    <dbReference type="EnsemblBacteria" id="CCZ83018">
        <property type="protein sequence ID" value="CCZ83018"/>
        <property type="gene ID" value="BN566_00811"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.980">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017856">
        <property type="entry name" value="Integrase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002876">
        <property type="entry name" value="Transcrip_reg_TACO1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026564">
        <property type="entry name" value="Transcrip_reg_TACO1-like_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029072">
        <property type="entry name" value="YebC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12532">
        <property type="entry name" value="PTHR12532"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01709">
        <property type="entry name" value="Transcrip_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75625">
        <property type="entry name" value="SSF75625"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01033">
        <property type="entry name" value="TIGR01033"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="195" mass="21581" checksum="CC73487E8097245A" modified="2013-07-24" version="1">MIAKAKSNNVPNDNIDRVIKKAAGGGDKNNYESIVYEGYGPNGVAVIVECLTDNKNRTAGDIRHYFDKFGGNLGTSGCVSFLFTEKGMVVLENTGLDEDTVMEDCFAFDADDLSVEDDVVEISCDPSVLPALRDGMTEKGYHVLSAEVEQIPATYTTLTDEDQIKKMNLLLEHLEENDDVQNVYHNWSMPEDIDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXM4</accession>
    <name>R5UXM4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00826</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000040">
        <property type="protein sequence ID" value="CCZ83033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83033">
        <property type="protein sequence ID" value="CCZ83033"/>
        <property type="gene ID" value="BN566_00826"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="66" mass="7369" checksum="4B77D4024A6BFD7C" modified="2013-07-24" version="1">MHHLLEMLLGLPLLHAAGYTTKQQMIDAYNSDLVLTLSEYQAWYAPKQTTTGESTVQTETTTTTIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXP6</accession>
    <name>R5UXP6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sugar phosphate isomerases/epimerases</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00856</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000050">
        <property type="protein sequence ID" value="CCZ83068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00856"/>
    <dbReference type="EnsemblBacteria" id="CCZ83068">
        <property type="protein sequence ID" value="CCZ83068"/>
        <property type="gene ID" value="BN566_00856"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="SSF51658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AP_endonuc_2" evidence="1">
        <location>
            <begin position="27"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="261" mass="29620" checksum="5914D7F48C415D24" modified="2013-07-24" version="1">MLKAGISTACLYPQPIEEALYDLALGGVTMVELFINTHSELKRSFVAALSDILHRFEVQCCSLHPFTSEIETMMLFSAYKRRLQDYLEYCRYYFTAMQQLGASIFVVHGNKQLVGVDVDFYCERFQKLVDLGKSYGVTVAQENVARCTSRSLSFLQEMQQKMGDDAKFVLDIKQAVRSKEDPMQMLKTLGSGVVHVHMSDHGEYGDCLQIGAGRFAVKSFLQTLAQLSPNCTIVLELYRSGFRTLQDLLHNDHILQRMIQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXP8</accession>
    <name>R5UXP8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">SWIM zinc finger domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00655</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000012">
        <property type="protein sequence ID" value="CCZ82850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00655"/>
    <dbReference type="EnsemblBacteria" id="CCZ82850">
        <property type="protein sequence ID" value="CCZ82850"/>
        <property type="gene ID" value="BN566_00655"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="2">
        <location>
            <begin position="48"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ82850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="533" mass="62688" checksum="57F6E016AB61C1D9" modified="2013-07-24" version="1">MNITDFEEYFSPRILERGKEYYRNHHVITLDRIEEGYYEAEVEGSQIYTVFVELEENGEISDISCDCPYDWEEFCKHEAAVLYALREQEEYTPFVEEPEQKQALPEQLAACEKETLIAWMMEYAKENRGFLDYLQLRVSEQLQPEELLAILNRTCNRFFSGKIVWKELQKTVAFLLKSMQSWNDDAEKVKTGASMIRLLKKWYQTNSCESDWIVYDEIDVCSDALMQIVQGVVKQKDRKAAEAIYQILLKMLQVDFIDIENPFSPMLNTLCALPEYRKKVEEWLQYRQAVSTEYERRELAEQQFQILKRYGTPQEQNDFINRHLSNPEYRRMAIQNAIDAGDESTVERLALDGEYENQALPGLLQEWQKCRYHCYHRTGEREKLADVCEALLKGGEPDYYEEWKSLIPFDLKSVKIEQLLKEAPIKVYRKILLAENRVDLMAEACEKDPSDLLLYFSALKCSPFAERATELYKDWIWEEADAAANRSEYAAVCQKLKKFSEDSPIAARVLAQELREKFPRRPAFQDELKKAGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXR5</accession>
    <name>R5UXR5_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cobyric acid synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">cobQ</name>
        <name evidence="6" type="ORF">BN566_00672</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 4">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CobB/CobQ family. CobQ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000019">
        <property type="protein sequence ID" value="CCZ82870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00672"/>
    <dbReference type="EnsemblBacteria" id="CCZ82870">
        <property type="protein sequence ID" value="CCZ82870"/>
        <property type="gene ID" value="BN566_00672"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015420">
        <property type="term" value="F:ABC-type vitamin B12 transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01750">
        <property type="entry name" value="GATase1_CobQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00028">
        <property type="entry name" value="CobQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033949">
        <property type="entry name" value="CobQ_GATase1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004459">
        <property type="entry name" value="CobQ_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011698">
        <property type="entry name" value="GATase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21343:SF1">
        <property type="entry name" value="PTHR21343:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07685">
        <property type="entry name" value="GATase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00313">
        <property type="entry name" value="cobQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="3 4" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GATase_3" evidence="5">
        <location>
            <begin position="253"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="332"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ82870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="504" mass="55110" checksum="E9562F57DA45C22F" modified="2013-07-24" version="1">MRAKSIMVVGTMSSAGKSVVTAGLCRMFHQDGYRVAPFKSQNMALNSYITKDGLEMGRAQVMQAEAAGIAPDIRMNPILLKPTSEAGSQVIVNGKVYDNLRAKEYYQRKQELIPEIRTAYESLAEEYDIIVLEGAGSPAEINLRDVDIVNMGMAAISDSPVIVVGDIDRGGVFASLYGTIALLRPEERCRIQGIIINKFRGDISILESGLTMLEELTGVPVLGVLPYLPLHLEEEDSLAEQLSVQTADGTGLDIAVIRLPRIANFTDFAALSGIEGVSVRYVTRRAELGTPDLLILPGTKNTLSDLRWLRESGLEAAILKLHEKGCLLFGICGGFQMLGKQVSDPEQTEGGGSLRGLGLLDMETIFQPQKHQQQVQGTIAEPSGVFAPLAGSAITGYEIHMGRSIAGSHAMPFTRRADGTLNGYCNEAGTVYGTYLHGFFDTAETTAALLQLLRKQTGRAEELSPVRDFQQEKERQYNQLADAIREHLDTKKIYQILEEWEQQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXU5</accession>
    <name>R5UXU5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AAA ATPase central domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00930</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family. RarA/MGS1/WRNIP1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000063">
        <property type="protein sequence ID" value="CCZ83143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00930"/>
    <dbReference type="EnsemblBacteria" id="CCZ83143">
        <property type="protein sequence ID" value="CCZ83143"/>
        <property type="gene ID" value="BN566_00930"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032423">
        <property type="entry name" value="AAA_assoc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021886">
        <property type="entry name" value="MgsA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16193">
        <property type="entry name" value="AAA_assoc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12002">
        <property type="entry name" value="MgsA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="SSF48019"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="2">
        <location>
            <begin position="37"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="424" mass="46679" checksum="D78004BA799537EE" modified="2013-07-24" version="1">MQPLAEQIRPTCLEEVVGQQHLLAPNRPLRRIIESGKIPNMIFYGPSGVGKTTVARMIANSTSMSLHKLNGTTASTADLREVAAEVGTLSGMHGILLYLDEIQYLNKKQQQSLLEYIESGDITLIASTTENPYFAVYNALISRSTVFEFKAVPPEELQKAVERAFSKVQKNFPVPITIAEGVCAHIAGGCGGDVRKAMNTVELCVLSAEKTESGLSVSLELAQELTQRSNMRYDRENDQHYDLLSALQKSIRGSDENAALHYAARLLAAGDLLSICRRLLVIASEDIGLAYPQAIVITKACVDSALQLGLPEAQIPLAEAVVLLATAPKSNSAYLAMHRAMEDVEKHGALDFPRHLQNCHFDGAGAAVKGQHYLYPHDYPNHYVPQQYLPDEIRDAVYYEFGTNKQEQAAALYRKKILEQANKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXV5</accession>
    <name>R5UXV5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00707</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000022">
        <property type="protein sequence ID" value="CCZ82905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00707"/>
    <dbReference type="EnsemblBacteria" id="CCZ82905">
        <property type="protein sequence ID" value="CCZ82905"/>
        <property type="gene ID" value="BN566_00707"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004394764" evidence="2">
        <location>
            <begin position="21"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="190"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="253"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="288"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="331"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ82905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="358" mass="38243" checksum="B410B481877FC979" modified="2013-07-24" version="1">MTAGTCILLLLLTVGCLVYAAPIHTALAEGVFLCLQVILPSLYAMMIAAQLLIHTNAHMLLLKPLHRFTQNVLRIPDVCLAIFLLSQIGGYPIGAKLLDNELQNGTLRPKQAAILSGICFGSGPAFLFGTLSAQAGTSGCWLLFGSILLANFSLFIIACRFLKLPNLPTVSLQRLQAKTLVETVTQSGAALLQMCAMILFFRCLMTVAQASGLYALLTIPLQWRFPKNTAEGLLSAGLEITALTQLVPNTPVLLALFAALLAFGGICVHLQIFTITGGRLSLYWMLSLRVLAGGLAAGFCWIGCQVFPNAVVSPEICSVSAPAVQYSRTTPIASLFLVGATLLLLQAAAKQRELCKHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5UY95</accession>
    <name>R5UY95_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">L-glutamine synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01079</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutamine synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01079"/>
    <dbReference type="EnsemblBacteria" id="CCZ83298">
        <property type="protein sequence ID" value="CCZ83298"/>
        <property type="gene ID" value="BN566_01079"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamate-ammonia ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006807">
        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040577">
        <property type="entry name" value="Gln-synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027303">
        <property type="entry name" value="Gln_synth_gly_rich_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022147">
        <property type="entry name" value="GSIII_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18318">
        <property type="entry name" value="Gln-synt_C-ter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12437">
        <property type="entry name" value="GSIII_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01230">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="SSF55931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00181">
        <property type="entry name" value="GLNA_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gln-synt_C" evidence="2">
        <location>
            <begin position="170"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="696" mass="77311" checksum="398A34BAEA4C6475" modified="2013-07-24" version="1">MSKNVAEYFACDVFNDEVMKARLPKAVYKALTKTRKLGVPLDPTYADVVANALKDWAIEHGATHYTHWFQPMTGSTAEKHDSFITPTDNGMVIMNFSGKELVKGEPDASSFPSGGLRATSSARGYTAWDPTSFCFVKEGSLYIPTAFVSYTGETLDKKTPLLRSMDVLSEQSIRILRLFGNTTATKVTSTVGPEQEYFLIDKAMYDKREDLIMTGRTLFGAMPPKGQELDDQYFANLKPRIRDYMADLDEKLWKLGVFATTKHNEVAPAQHEMAPVFSTTNISTDQNELAMEVMKKLAVKHGLVCLLHEKPFEGVNGSGKHNNWSIATNDGQNLLNPGSTPMENLQFLTFLCAVIKGVDEYADLLRISAASAANDHRLGANEAPPAIISIFMGEELQGVLDALVSGDTYTSQSMTFKIGVDALPDFPKDSTDRNRTSPFAFTGNRFEFRMVGSSENIACPNHMLNTIVASELSDIADKMEKVPADKFKDELKKLLAEIVKEHKRVIFNGDGYSDAWVEEAAKRGLPNYKTTADCVPHYADEKNVKLFEKFGIFSKAEIEARVEILLESYSKTINIEALTMIDMAKKKYIPACLTYSKELADAIAVKKSIDVCTCVEKDLVKKVTEETEKAYDAVQKLEEVTAVAAAKTCMQEMANAYRDTVLPVMDELRKAVDALEVLLPSTMWPVPAYSEMIFNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYA0</accession>
    <name>R5UYA0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted amidophosphoribosyltransferases</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01084</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01084"/>
    <dbReference type="EnsemblBacteria" id="CCZ83303">
        <property type="protein sequence ID" value="CCZ83303"/>
        <property type="gene ID" value="BN566_01084"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044005">
        <property type="entry name" value="DZR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18912">
        <property type="entry name" value="DZR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DZR_2" evidence="2">
        <location>
            <begin position="14"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="Pribosyltran" evidence="1">
        <location>
            <begin position="174"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="237" mass="26401" checksum="E7EEEE75E53A13DC" modified="2013-07-24" version="1">MKQTHLLQNSYRFLIDLVYPNRCPCCNQVIAWDAWICEACQIETALNPDTVCSGCGKPFDDCMCSESLAFDGAMTVSRYEGRARQGILQLKQAENLNFANYTGTVLGERILQKPDWMMADAIVPVPMQQHQWILRGKNPAERIANAISFVTKIPVRTDLLWKKPGGIPQHTLNAEQRRTNVLQFASAGKSLKGYIFILCDDVLTTGSTANYCASLLKQCGAERVYVVTATTTTKIKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYF5</accession>
    <name>R5UYF5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phage tail tape measure protein TP901 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00848</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000045">
        <property type="protein sequence ID" value="CCZ83055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00848"/>
    <dbReference type="EnsemblBacteria" id="CCZ83055">
        <property type="protein sequence ID" value="CCZ83055"/>
        <property type="gene ID" value="BN566_00848"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010090">
        <property type="entry name" value="Phage_tape_meas"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10145">
        <property type="entry name" value="PhageMin_Tail"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="SSF48371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01760">
        <property type="entry name" value="tape_meas_TP901"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="210"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="259"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="PhageMin_Tail" evidence="2">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="902" mass="98569" checksum="9FFFA449C53751AD" modified="2013-07-24" version="1">MGETFKYAAPVLGSLGYSAEDSAIAIGLMANAGIKSSQAGTALRGAIVSLAKPTDTVSSAMEKYGISLTDSSGKMYSLRDLMGQMRDKLGGLTEAEQAQAAASLFGREAMSGMLAIINASPADFEKLTNAVDTCSDTVDGYNGTTEKMAATMQDNLAGQLTILKSQLEELAISFSDILMPAIRSLVARLQTIVDKLNQLDPQVKETIVKIALAVAAISPLLIAIGKVISVVGTLMQAIAKIPKILGSIKNGFSAVTGALKVSTAGFSAAVGVIALLAAAFVHLWQTNEDFRNKIISIWEQIKGTFTELTQGITDRLNALGFDFDDFGEAVKAAWEGFCNLLAPIFEGAFEIVAEIFEAVSGTILGLMDVFTGVFSGDWEQAWTGVKEIFSSIWEGIKSILQTILDTLKGVANTFLGWFGTDWETVWTSVKAFFTNTWTNIQTFFSNTLTNIKTFFSNIWTSISTTVTNVLTTIQTTVTNIFTAIQTFVTTIWQGIYTFFSTIFNAIYTVVYTVFNTIYTVITTVWTTIYTTLEPLINAFGYLFETIFEAIQIVVGRVMDWISEKISAIWNGIVDFITPILESIRDFFSEIWTAISDKVQEKLEFIKNLVETIWNGIKDFLEPLLTALQTTFTNIWEAIRSQIDAVSNAIRSLIERIWSSISGTISSVMDSIRNTFSSIWDSISDKISSVVNGIKTNVSNAWENIYDSISNLMSQIKNKISDIWDGIHDGISDKIGDIRTTIENGLNGAINWIRGLASDAWNWGSDIIWGIIDGIQSAIGWLADCVTNVADIIRDFLHFSVPDKGPLTDYESWMPDFMQGLADGINKSKKIVTQAVAAVADGISVSMQGNLQMDALKSEQGSAGATTTVINNDNSRTINQTNNSPKALTRLEIYRQTRNAINV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYL4</accession>
    <name>R5UYL4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Cysteine synthase</fullName>
            <ecNumber evidence="5 11">2.5.1.47</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BN566_00907</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>hydrogen sulfide + O-acetyl-L-serine = acetate + L-cysteine</text>
            <dbReference type="Rhea" id="RHEA:14829"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:58340"/>
            <dbReference type="EC" id="2.5.1.47"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 9 11">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the cysteine synthase/cystathionine beta-synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.47" evidence="5 11"/>
    <dbReference type="EMBL" id="CBAR010000060">
        <property type="protein sequence ID" value="CCZ83120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00907"/>
    <dbReference type="EnsemblBacteria" id="CCZ83120">
        <property type="protein sequence ID" value="CCZ83120"/>
        <property type="gene ID" value="BN566_00907"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00136">
        <property type="reaction ID" value="UER00200"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004124">
        <property type="term" value="F:cysteine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005856">
        <property type="entry name" value="Cys_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005859">
        <property type="entry name" value="CysK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001216">
        <property type="entry name" value="P-phosphate_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01139">
        <property type="entry name" value="cysK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01136">
        <property type="entry name" value="cysKM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00901">
        <property type="entry name" value="CYS_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8 11" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="7 9 11" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PALP" evidence="12">
        <location>
            <begin position="7"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="9">
        <location>
            <begin position="180"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="10">
        <location>
            <position position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR605856-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR605856-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CCZ83120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="309" mass="32916" checksum="983375C4682E156F" modified="2013-07-24" version="1">MKIVQKITDLIGHTPLLELTHTEQEEQLQATLLGKLEYFNPAGSVKDRIAKAMIDEAEEQGLLHPDSVLIEPTSGNTGIGLASVAAARGYRLIITMPETMSVERRNLMKAYGAELVLTEGAKGMKGAIEKADELAVQIPNSFIPSQFTNPANPEAHKKTTGVEIWDDTDGKVDIFVAGVGTGGTISGVGAYLKEKNPNVKIVAVEPKSSPVLSEGFAGAHKIQGIGAGFVPETLDTKIYDEVIAVENEAAFETGRKIARNEGVLVGISSGAAVWAAIQLAKRPENKGKVIVALLPDTGERYLSTPMFAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYM8</accession>
    <name>R5UYM8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Beta-mannanase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_01194</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 26 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000099">
        <property type="protein sequence ID" value="CCZ83418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01194"/>
    <dbReference type="EnsemblBacteria" id="CCZ83418">
        <property type="protein sequence ID" value="CCZ83418"/>
        <property type="gene ID" value="BN566_01194"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016985">
        <property type="term" value="F:mannan endo-1,4-beta-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006080">
        <property type="term" value="P:substituted mannan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022790">
        <property type="entry name" value="GH26_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000805">
        <property type="entry name" value="Glyco_hydro_26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40079">
        <property type="entry name" value="PTHR40079"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02156">
        <property type="entry name" value="Glyco_hydro_26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00739">
        <property type="entry name" value="GLHYDRLASE26"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51764">
        <property type="entry name" value="GH26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GH26" evidence="5">
        <location>
            <begin position="13"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="171"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ83418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="320" mass="36551" checksum="4F9A3CE986D1C9A0" modified="2013-07-24" version="1">MIHTTPVNPNATPEATALLQYLHDIAGTKLIAGQHTQTNPMEEVAYIETVTGKSPKLCGFELLAYSPNIRMEGASEACRVEVEENRNTMDTALRWANETGGIVTLTFHWFSPLGGHDKSFYTEHTNFDAERVLHDDTPERAAFFHDLDAIAVQLQRFQDAKIPILWRPFHEAEGTWFWWGAKGAEVAKQLYLLMYEYYVHHYHFDHLLWVWNSRLPEGYPGDDFVDVVSVDVYLPHYEATDYAADYQALIAASSANKVAALAEVGFLPDAALLAKSKIPWAYFMTWSKEFCIGETYNTRQRLKDVYNHERVLSQKPVLTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYT4</accession>
    <name>R5UYT4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ribonucleoside-triphosphate reductase class III catalytic subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_00983</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000070">
        <property type="protein sequence ID" value="CCZ83200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00983"/>
    <dbReference type="EnsemblBacteria" id="CCZ83200">
        <property type="protein sequence ID" value="CCZ83200"/>
        <property type="gene ID" value="BN566_00983"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008998">
        <property type="term" value="F:ribonucleoside-triphosphate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01675">
        <property type="entry name" value="RNR_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005144">
        <property type="entry name" value="ATP-cone_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012833">
        <property type="entry name" value="NrdD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03477">
        <property type="entry name" value="ATP-cone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13597">
        <property type="entry name" value="NRDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02487">
        <property type="entry name" value="NrdD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51161">
        <property type="entry name" value="ATP_CONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-cone" evidence="4">
        <location>
            <begin position="3"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="772" mass="86382" checksum="90C433000D33632C" modified="2013-07-24" version="1">MISYIIKRDGRRESFDLDKIANAVFRAAQSVGGTDAAMARDVAEKTCALYEQLHGSQEPTVEEIQDLVEKELIECGHAQTAKAYILYRYERTRDREVKSNLMKIMNELTFDSAKDSDIKRENANIDGDTAMGTMLKYGSSAAKEFYEMRVLKPEHAKAHRNGDIHIHDLDFLTLTTTCCQIDLIQLFQHGFSTGHGFLREPNDISSYSALACIAIQSNQNDQHGGQSVPNFDYAMAGGVKKTYRKRYLQNVARALELLTDIEEPQAFVKSYAAAIQQETGLIPCLANNNGYQEAEAQKLSERLTADQIATIQAFAKKNAYRETNRATYQAMEALIHNLNTMNSRAGAQTPFSSINYGTDTTPEGRMVIENVLLAEEAGLGNGETPIFPIHIFKVKEGVNYNKEDPNYDMFKLACRVSAKRLFPNFAFLDAPYNLQYYKPGDYRTEIAYMGCRTRVIGNEYDPTREIVTGRGNLSFTSINLPRLAIKANGDIDKFFTLLNNMMDLTIEQLTDRFAIQCQKKVRNYPFLMGQGVWLDSEGLGPDDSVAEVLKHGTLSVGFIGLAETLKALIGKHHGESAEAQELGLKIIQTMRDRTDLESKKTGLNFSVLATPAEGLSGRFVRMDRERYGVIPGVTDRDYYTNSFHVPVYYPIRAVDKIRLEAPYHALTNGGHISYVEMDGDPVQNVAAFEKVIRCMKEAGIGYGSVNHPVDRDPCCGFTGIIGDSCPQCGRTEHSGDVGFERIRRITGYLVGTLDRFNDAKRAEEHDRVKHAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYZ5</accession>
    <name>R5UYZ5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01051</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01051"/>
    <dbReference type="EnsemblBacteria" id="CCZ83270">
        <property type="protein sequence ID" value="CCZ83270"/>
        <property type="gene ID" value="BN566_01051"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008641">
        <property type="term" value="F:ubiquitin-like modifier activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43267">
        <property type="entry name" value="PTHR43267"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="SSF69572"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ThiF" evidence="1">
        <location>
            <begin position="11"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="254" mass="27974" checksum="A13B45B58B377509" modified="2013-07-24" version="1">MLNQFSRTELLYGKAVMEHFQKSRVAVFGIGGVGGYAVEALARSGIGTLDLIDDDKVCITNLNRQLFALRSTVGKYKVDAAAERIQDICPSTVVHRHKCFYLPETADQFDFRQYDYVIDAIDTVTGKLEIIQQAKNAGVPVISAMGAGNKLDPTAFRVADIYETKMCPLARVMRNALRKRGIKSLKVVYSEEQPRRPLDDSENSCRTQCVCPPGTVRKCTVRRDIPGSTAFVPSVVGLIVAGEVLNDLAAQVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZ02</accession>
    <name>R5UZ02_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thioredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01061</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thioredoxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01061"/>
    <dbReference type="EnsemblBacteria" id="CCZ83280">
        <property type="protein sequence ID" value="CCZ83280"/>
        <property type="gene ID" value="BN566_01061"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005746">
        <property type="entry name" value="Thioredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000077">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="3">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="2">
        <location>
            <begin position="30"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR000077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000077-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="106" mass="11422" checksum="34B7C579244ECCA5" modified="2013-07-24" version="1">MAVRVNAETFAAEVLQAEGLVLVDFYSDSCVPCKRIAPLLSEVAAADPDGLKVVKVNINFDAELAEQYAVQAAPTLVFFQNGAEVARLRGLVKKAELTEVIDSLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZK2</accession>
    <name>R5UZK2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01518</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000161">
        <property type="protein sequence ID" value="CCZ83783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01518"/>
    <dbReference type="EnsemblBacteria" id="CCZ83783">
        <property type="protein sequence ID" value="CCZ83783"/>
        <property type="gene ID" value="BN566_01518"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023975">
        <property type="entry name" value="Six-Cys_pep_SCIFF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13165">
        <property type="entry name" value="SCIFF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03973">
        <property type="entry name" value="six_Cys_in_45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="45" mass="4760" checksum="6BCD7FF4CD2D256B" modified="2013-07-24" version="1">MKHIKTINKANLKESAAKGGCGQCQASCQSACKTSCTVANQKCER</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZL1</accession>
    <name>R5UZL1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01528</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000162">
        <property type="protein sequence ID" value="CCZ83793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83793">
        <property type="protein sequence ID" value="CCZ83793"/>
        <property type="gene ID" value="BN566_01528"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="149" mass="17255" checksum="725D6412DA0E8D21" modified="2013-07-24" version="1">MKWKHWKKWALVGGIILIGCSARFLSISQIEKNSVITISDSDVFQQWEIRAAIQQTAADFTIWHPLSPQIHATAQTFSYEESLCQTEWKCSNYGCKRENFIVISCTFLTDETASRTVFDMQDNMVYSQTWIFTRKAKFLPWVLQSQGEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>R5V059</accession>
    <name>R5V059_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">6,7-dimethyl-8-ribityllumazine synthase</fullName>
            <shortName evidence="7">DMRL synthase</shortName>
            <shortName evidence="7">LS</shortName>
            <shortName evidence="7">Lumazine synthase</shortName>
            <ecNumber evidence="4 7">2.5.1.78</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ribH</name>
        <name evidence="8" type="ORF">BN566_01702</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>(2S)-2-hydroxy-3-oxobutyl phosphate + 5-amino-6-(D-ribitylamino)uracil = 6,7-dimethyl-8-(1-D-ribityl)lumazine + H(+) + 2 H2O + phosphate</text>
            <dbReference type="Rhea" id="RHEA:26152"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58201"/>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
            <dbReference type="EC" id="2.5.1.78"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the DMRL synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.78" evidence="4 7"/>
    <dbReference type="EMBL" id="CBAR010000178">
        <property type="protein sequence ID" value="CCZ83973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01702"/>
    <dbReference type="EnsemblBacteria" id="CCZ83973">
        <property type="protein sequence ID" value="CCZ83973"/>
        <property type="gene ID" value="BN566_01702"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00404"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009349">
        <property type="term" value="C:riboflavin synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000906">
        <property type="term" value="F:6,7-dimethyl-8-ribityllumazine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09209">
        <property type="entry name" value="Lumazine_synthase-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.960">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00178">
        <property type="entry name" value="Lumazine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034964">
        <property type="entry name" value="LS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002180">
        <property type="entry name" value="LS/RS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036467">
        <property type="entry name" value="LS/RS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21058">
        <property type="entry name" value="PTHR21058"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00885">
        <property type="entry name" value="DMRL_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52121">
        <property type="entry name" value="SSF52121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00114">
        <property type="entry name" value="lumazine-synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0686">Riboflavin biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="5-amino-6-(D-ribitylamino)uracil binding" evidence="7">
        <location>
            <begin position="56"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="5-amino-6-(D-ribitylamino)uracil binding" evidence="7">
        <location>
            <begin position="80"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="1-deoxy-L-glycero-tetrulose 4-phosphate binding" evidence="7">
        <location>
            <begin position="85"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>5-amino-6-(D-ribitylamino)uracil</name>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>5-amino-6-(D-ribitylamino)uracil</name>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>(2S)-2-hydroxy-3-oxobutyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="153" mass="16323" checksum="8C7A4F3CEE8BE7E2" modified="2013-07-24" version="1">MKKIEGMYEGEGLKVAIVASRFNEFITTKLVGGAEDCLLRHGVNADDIALAWVPGAFEIPLIAKKLADSKKYDAVICVGAVIRGATSHYDYVCAEVSKGIAQVGLQSEIPVLFGVLTTDTIEQAIERAGTKAGNKGYDCAMSALEMANLLKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5V060</accession>
    <name>R5V060_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 12">Epoxyqueuosine reductase QueH</fullName>
            <ecNumber evidence="5 12">1.17.99.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 12">Queuosine biosynthesis protein QueH</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">queH</name>
        <name evidence="13" type="ORF">BN566_00162</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 12">Catalyzes the conversion of epoxyqueuosine (oQ) to queuosine (Q), which is a hypermodified base found in the wobble positions of tRNA(Asp), tRNA(Asn), tRNA(His) and tRNA(Tyr).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>AH2 + epoxyqueuosine(34) in tRNA = A + H2O + queuosine(34) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:32159"/>
            <dbReference type="Rhea" id="RHEA-COMP:10345"/>
            <dbReference type="Rhea" id="RHEA-COMP:10346"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:82831"/>
            <dbReference type="ChEBI" id="CHEBI:82834"/>
            <dbReference type="EC" id="1.17.99.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">tRNA modification; tRNA-queuosine biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the QueH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.17.99.6" evidence="5 12"/>
    <dbReference type="EMBL" id="CBAR010000146">
        <property type="protein sequence ID" value="CCZ83635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00162"/>
    <dbReference type="EnsemblBacteria" id="CCZ83635">
        <property type="protein sequence ID" value="CCZ83635"/>
        <property type="gene ID" value="BN566_00162"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00392"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052693">
        <property type="term" value="F:epoxyqueuosine reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008616">
        <property type="term" value="P:queuosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02089">
        <property type="entry name" value="QueH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003828">
        <property type="entry name" value="QueH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36701">
        <property type="entry name" value="PTHR36701"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02677">
        <property type="entry name" value="QueH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="10 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9 12" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-0671">Queuosine biosynthesis</keyword>
    <keyword evidence="12" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0819">tRNA processing</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="12">
        <location>
            <begin position="197"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00016895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CCZ83635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="211" mass="24145" checksum="378F9B3E7EE13002" modified="2013-07-24" version="1">MQTPPKPNYQRELEQTLSTIPTGTKPHLFLHACCAPCSSYVLEYLSRYFTITIYFYNPNIMPEAEFSHRLAELRRLLQEMPLEGTVSVVAGTWEPERFLALAKGHEHEPERGERCQRCIRLRLEQTMQAAKAAHADFVTTTLSISPHKDASFINQCGAELAKQFDVPWLYADFKKKGGYQRSIALSHQYDLYRQDFCGCVFSAANRHAASH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0D3</accession>
    <name>R5V0D3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ABC transmembrane type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_01465</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000158">
        <property type="protein sequence ID" value="CCZ83730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01465"/>
    <dbReference type="EnsemblBacteria" id="CCZ83730">
        <property type="protein sequence ID" value="CCZ83730"/>
        <property type="gene ID" value="BN566_01465"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="145"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="246"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="75"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ83730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="282" mass="31280" checksum="146AA1B21752EBE3" modified="2013-07-24" version="1">MRKTKKIGGSNVLVTVLLFLGCLTILFPLYMTIIIAFKNPSEMTNDVAGALAFPSSWKLDNFKEAMEVTNFWHTLGNSLLITIATIVLALLIHSLAGYVIGRNMARKKGYRFIYFYIVSGMFVPFAILMMPLVKETAILGLDNRLGVILLYLVFYMPINVMLYSGYLKNIPLAMEEAAEIDGASAWKTYWTVIFPMMKPMHATVAVLTALGTWNDVMTPLVIMSGTKGTTLPLAQLNFQTQFGTNYNLAFASYLLALIPILIFYVICQKQILNGVANGAVKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0E2</accession>
    <name>R5V0E2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized FAD-dependent dehydrogenases</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01769</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000181">
        <property type="protein sequence ID" value="CCZ84043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01769"/>
    <dbReference type="EnsemblBacteria" id="CCZ84043">
        <property type="protein sequence ID" value="CCZ84043"/>
        <property type="gene ID" value="BN566_01769"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028348">
        <property type="entry name" value="FAD-binding_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42842">
        <property type="entry name" value="PTHR42842"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038984">
        <property type="entry name" value="FAD_binding_protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="535" mass="58970" checksum="3D673E9490BEB8C7" modified="2013-07-24" version="1">MIKISEIKMPVGYTEEMVTASILKKLHLKNDTALTWNYFKKSIDARKKQQIQYQISVLVSHPNEQKLLQQASAKKEKNITAYQPYVYPIPSISKRPENRPVVVGFGPAGMFAALVLAKAGLQPLVLERGKCVEERKKDVLDFWENGILHTDSNIQFGEGGAGTFSDGKLTTGIKDSRIRFILETFVACGAPSEILYLAKPHIGTDYLEQVVKNIRCKIQELGGEILFSARFMGYQTDEHGRLKSISYEKEGSIREQQTQHCILAIGHSARDVFQLFYDKNIPLQQKNFAMGVRIEHLQSDINKSMYGTFAKQLPAADYKLAVHLPSGYDLYTFCMCPGGNVVAAASEEGHLVVNGMSNHARDERNANSALLVGISPRVLASEHPLAGMFLQQKLEQQAFLSGGGNYAAPVSCVGDFLQNQTSSSFGKVTPTYRLGVKFVNPREYLPEFMVQTLKDGILEMGKKIAGFDDPEAILTGVETRSSSPVRILRNEKLECIAVAGLYPCGEGAGYAGGITSAAVDGIKCAEKLIEKIENN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0I8</accession>
    <name>R5V0I8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_01829</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the type-I restriction system S methylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000187">
        <property type="protein sequence ID" value="CCZ84103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01829"/>
    <dbReference type="EnsemblBacteria" id="CCZ84103">
        <property type="protein sequence ID" value="CCZ84103"/>
        <property type="gene ID" value="BN566_01829"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.220.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000055">
        <property type="entry name" value="Restrct_endonuc_typeI_TRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044946">
        <property type="entry name" value="Restrct_endonuc_typeI_TRD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01420">
        <property type="entry name" value="Methylase_S"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0680">Restriction system</keyword>
    <feature type="domain" description="Methylase_S" evidence="4">
        <location>
            <begin position="4"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="Methylase_S" evidence="4">
        <location>
            <begin position="179"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ84103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="371" mass="42212" checksum="ADC395826CBEF5BB" modified="2013-07-24" version="1">MKTTVKLKDIASYSKGSQINGNNLIEDGAYEYLNGGINPSGKWNEYNVDGNTITISEGGNSCGYVNYMINPFWCGAHCYYLYNLKCSSKYLYYALKGSQSSIMKLRSGACMPNIKKKDLGEFILSIDLDKKEQDKTIKIFNKLQSIITHLSTQLEKLDLLVKARFVEIFGDTEYNTKKFPVYKLSELCTVSSSKRIYQDEQSSTGVPFLRISNLVELIETGHLKNELFISDEKYIELVRSNLVPQKGDILVTSRGTLGKCYIVQDDDKFYFQDGMISWLYNLNKAITPLYLALLFNTRNIKAQIDNLQSGSTVAYLSITMLKKLNIVVPPLTLQNQFADFVKQIDKSKSILQQELDKAQMLFDSLMQEYFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0I9</accession>
    <name>R5V0I9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01520</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000161">
        <property type="protein sequence ID" value="CCZ83785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83785">
        <property type="protein sequence ID" value="CCZ83785"/>
        <property type="gene ID" value="BN566_01520"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002105">
        <property type="entry name" value="Dockerin_1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00404">
        <property type="entry name" value="Dockerin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004394891" evidence="1">
        <location>
            <begin position="30"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="3">
        <location>
            <begin position="267"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="304"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="344" mass="37515" checksum="FEF6D471DDD5B3D3" modified="2013-07-24" version="1">MIFMKKKGFVAAAMAAVLASSMLSVPAFAWSKDDIIAGDEHTLIVSYHWSDIDRLTIGDTQDGTHFITSHNLNCIAIVMEDENTVPDMTTISSDLTAVPAESYQFDGLYEYWNNQIATLFSPLLNLKTTYFVSASEQDAEKFYQLSGVEAVYEIHSEAEHETWIGDGTSASINVSVKVPKGTDFGVEQCADLPYTVSSVTEIESTDDAMDAYKLVVKVPDGKIYKAALDMIRTLLEEDIVPDASVSYMMTELALAGKPVLKEVPNHYLAANSDLDGDGIVDVQDAVELLTYYARKAANLPASFSHLDDQEAALQLADVNQDGTVDAADAVEILTYYTKQAAGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0M7</accession>
    <name>R5V0M7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Amino acid carrier protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN566_01873</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the alanine or glycine:cation symporter (AGCS) (TC 2.A.25) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000192">
        <property type="protein sequence ID" value="CCZ84148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01873"/>
    <dbReference type="EnsemblBacteria" id="CCZ84148">
        <property type="protein sequence ID" value="CCZ84148"/>
        <property type="gene ID" value="BN566_01873"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015655">
        <property type="term" value="F:alanine:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001463">
        <property type="entry name" value="Na/Ala_symport"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30330">
        <property type="entry name" value="PTHR30330"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01235">
        <property type="entry name" value="Na_Ala_symp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00175">
        <property type="entry name" value="NAALASMPORT"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00835">
        <property type="entry name" value="agcS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00873">
        <property type="entry name" value="NA_ALANINE_SYMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0769">Symport</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="172"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="201"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="339"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="371"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="397"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ84148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="439" mass="46418" checksum="3E45F246F50005E0" modified="2013-07-24" version="1">MLEQLNTWVWGSGLLVLLLGTGLFLSIRTHFFQIFGIRTILRQTFGRLFRRQDGAEQWKTCSAALAAAMGTGNIVGVAAAISAGGAGAVFWMWVSAFLGMMLTYAENVLAVRYRSETEKRAVGALAYLKNGLRSPILAGLYAVFCVLASFGMGNMTQSSAAAAALEHGCHIPPIWTGVGVTILLLGTVLGGMRTIGTVTQFLMPILSGGYLLAAVIVLVQHAGQLGTAFATIFHAAWHPAAVGGGTLGTVLSAGLRHGVFSNEAGLGSSAMIHSHAVDAVHGQQGMWSMVEVFVDTMLCCTVTALVLLCTGTAGTDGISGIAVAFSSVFGIGAEPVLSWMIALFALATLLGWCCCGEVAVRYLGGERGVRWYRWAYCFAGGLGAVGTLSTIWTFSDLANGLMAIPNLLGILLLFRKTDLPDNVYRKCTKKNCKKKKNMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0Q4</accession>
    <name>R5V0Q4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">D-alanyl-D-alanine carboxypeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01903</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01903"/>
    <dbReference type="EnsemblBacteria" id="CCZ84178">
        <property type="protein sequence ID" value="CCZ84178"/>
        <property type="gene ID" value="BN566_01903"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1380.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009045">
        <property type="entry name" value="Hedgehog_sig/DD-Pept_Zn-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003709">
        <property type="entry name" value="Pept_M15B"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02557">
        <property type="entry name" value="VanY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55166">
        <property type="entry name" value="SSF55166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="VanY" evidence="3">
        <location>
            <begin position="185"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="330"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="341"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="370" mass="40796" checksum="7BF5A2C1F31AA963" modified="2013-07-24" version="1">MKQKTKKRPQIVSVVIALACLICLILVIDFVVRTVTRANVTYVVSEGTFSTDTNNGIPEATDKPTEAKGNKKDNETVATTKNDDPNYDTITLSDSDLHNGSLVMVSNTCPYSGKTDFTDFSTLSDTSLKPRSESLTFQTEMRNPLINLFDAYQASQGTVNLQIHSTSVTNTDSDSLYTNVLAERSSGYTFDIGLITSTGEVVAYLTKRNEWMVANCWYYGFILRYPDDKTSITGVDYMPHHFRYVGLPHSLIMYENNYCLEEYLDYLKSYTIDKPLTYTTDDASYEIYYYAANSNGETEVKIPKKDSYSISGNNTDGFIITVQKSAGEISGEAATDAATQDDNADAATGDTATSNDSEAATTEAAADNEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0T7</accession>
    <name>R5V0T7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator GntR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01614</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000169">
        <property type="protein sequence ID" value="CCZ83885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01614"/>
    <dbReference type="EnsemblBacteria" id="CCZ83885">
        <property type="protein sequence ID" value="CCZ83885"/>
        <property type="gene ID" value="BN566_01614"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="1">
        <location>
            <begin position="11"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="132" mass="15130" checksum="4D3F5A03C614E53A" modified="2013-07-24" version="1">MQLMIRNNTAAPIYQQIYQQIKEQIINGTLEEDTMLPSIRSLAKELRISIITTKRAYEELERDGFVYTIAGKGVFVARKNTEVLREEWLRQIESHMEAIATLAKGCGLSKADLQEMLNMIYEEDASCNMPSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0Y3</accession>
    <name>R5V0Y3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">N-(5'-phosphoribosyl)anthranilate isomerase</fullName>
            <shortName evidence="4">PRAI</shortName>
            <ecNumber evidence="2 4">5.3.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">trpF</name>
        <name evidence="6" type="ORF">BN566_01659</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:21540"/>
            <dbReference type="ChEBI" id="CHEBI:18277"/>
            <dbReference type="ChEBI" id="CHEBI:58613"/>
            <dbReference type="EC" id="5.3.1.24"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 4">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TrpF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.24" evidence="2 4"/>
    <dbReference type="EMBL" id="CBAR010000173">
        <property type="protein sequence ID" value="CCZ83930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01659"/>
    <dbReference type="EnsemblBacteria" id="CCZ83930">
        <property type="protein sequence ID" value="CCZ83930"/>
        <property type="gene ID" value="BN566_01659"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00042"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004640">
        <property type="term" value="F:phosphoribosylanthranilate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00405">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00135">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001240">
        <property type="entry name" value="PRAI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044643">
        <property type="entry name" value="TrpF_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42894">
        <property type="entry name" value="PTHR42894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00697">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="4 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="PRAI" evidence="5">
        <location>
            <begin position="4"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ83930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="205" mass="22233" checksum="6F868584DC8E84D7" modified="2013-07-24" version="1">MKIKFCGIRRLEDVTAVNLCQPDYMGMILSGGFRRSISQEQAQRLVQEKSAAIAAVGVFVNESSETICHMAEQLHLQVIQLHGNESAEQIQTLQQKTGLPVWKALRIGTLEELEAAGTNPADCLILEGKTGAGIGGTGVCADWELLARHSWNRPFFLAGGLQPENVLEAIATVSPTGVDFSSGIEEDGVKSLRRMKQLMTLIRGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>R5V102</accession>
    <name>R5V102_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GntR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01674</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000174">
        <property type="protein sequence ID" value="CCZ83945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01674"/>
    <dbReference type="EnsemblBacteria" id="CCZ83945">
        <property type="protein sequence ID" value="CCZ83945"/>
        <property type="gene ID" value="BN566_01674"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="1">
        <location>
            <begin position="10"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="127" mass="14429" checksum="664E1F4E441239BF" modified="2013-07-24" version="1">MFQIDSMSRKPVYEQLIEQVESFLLNDILKPGDQIPSVRNVTMQHAINPRTVLKAYNDLDARGLIRSVPGKGYFVCEEAKQILNQRHMERLQELTEILKEMALAGVPLAAIQDCVNKAYAPKKEETS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5V112</accession>
    <name>R5V112_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">CO dehydrogenase/acetyl-CoA synthase alpha subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_01684</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000175">
        <property type="protein sequence ID" value="CCZ83955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01684"/>
    <dbReference type="EnsemblBacteria" id="CCZ83955">
        <property type="protein sequence ID" value="CCZ83955"/>
        <property type="gene ID" value="BN566_01684"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006058">
        <property type="entry name" value="2Fe2S_fd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="SSF54292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00197">
        <property type="entry name" value="2FE2S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="5">
        <location>
            <begin position="3"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="138"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ83955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="224" mass="25384" checksum="D9F203B8B6999E3B" modified="2013-07-24" version="1">MEQMVNVFIMGKQYKVPASLTIMKAFEYAGYKLIRGCGCRNGFCGACATVYRIKGDRELKVCLACSTQVEEGMYLTQLPFYPLIKELYNIEEVKPTEQLMMQLYPEIYSCIGCNSCTNSCTQGLNVMQYIAYAQRGEYAKCAEESFDCVMCGCCSSRCPAGISHPQVAMLARRLNGKYIEPECKHLKERVEEIAEGKCEEAIRANMALSDAEIREMYNNREIEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1A0</accession>
    <name>R5V1A0_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Stage 0 sporulation protein A homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN566_01739</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000178">
        <property type="protein sequence ID" value="CCZ84010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01739"/>
    <dbReference type="EnsemblBacteria" id="CCZ84010">
        <property type="protein sequence ID" value="CCZ84010"/>
        <property type="gene ID" value="BN566_01739"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Response regulatory" evidence="7">
        <location>
            <begin position="3"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="8">
        <location>
            <begin position="125"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="6">
        <location>
            <begin position="125"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="5">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ84010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="227" mass="26038" checksum="C6E67D78E30E3944" modified="2013-07-24" version="1">MQRILIIEDDTTIRNGIQLYLQKKEFAVDCADTIAAANTLFAKMVYHLILLDIRLPDGNGLELCSKLRETSTTPIIFLTANDTEEQMITGFQHGCDDYLAKPFSIALLYQRILAVLRRSQNPASDDIFSYLSLTIDFTKMQVLKNDVPVKLSAMEYKLLELLIRNRGQVLTRDLILERLWDCDGNFVDENTLRVLIRRLRQKIETDPKNPKFIVTVFGIGYTFGEAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1C4</accession>
    <name>R5V1C4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ATPase components of ABC transporters with duplicated ATPase domains</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_00290</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000222">
        <property type="protein sequence ID" value="CCZ84383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00290"/>
    <dbReference type="EnsemblBacteria" id="CCZ84383">
        <property type="protein sequence ID" value="CCZ84383"/>
        <property type="gene ID" value="BN566_00290"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032781">
        <property type="entry name" value="ABC_tran_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12848">
        <property type="entry name" value="ABC_tran_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="3"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="327"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="98"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="532"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="35"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="363"/>
            <end position="370"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ84383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="640" mass="72911" checksum="94CD6FA7C925F59E" modified="2013-07-24" version="1">MLLQLTDVNKIYNGAPLLKHISLTIEDRDRIGLIGVNGCGKSTLLKLITERILPDHIQEGDGEIIKSSHTSIGYLEQMGGLEKEQTVWHEMHNVFQPLLDAQARMRELEQELANNSEAAAEEYHRLQTYFESNDGYQIDVKIKMVLNGMGFGAETYERKISSFSGGEKTRLAIAKLLLEEPTLLILDEPTNHLDFKTVMWLEDYLKDYKGALLLVSHDRYFLNRLCTSICEIEHGVLTRYKGDYSAYTVQKEAAVIRQTKEYELQQKEIAKLEDYIARNQTRASTAKSAQSRMKQLEKMERIEKPITYQKSARVQFTYDIEPPQQVLQVQDIAIAVGSGAERKVLAEPVSFEVRRGEKWGIIGENGVGKSTLLKILQGQLPHVGKIRWAAQTKISYFEQESTNLHPAKTVMQEIHDRVPTWLDYDVRSLLGKVRITGEEVYKQVGVLSGGERAKVCFAVMMLEHGNILILDEPTNHLDISMKEVIEDAMKTFTGTILFVSHDRYLLDQVADHILEITPTGVKTYIGGFQSWLDATKKEAAAANAAAQAVETEKKKEDKQQMYRSKEQRAKAAQQRARMRQLEQQSEAMQAELDALNEEITKEEVYSDFNLMQEKCKRMDTLRTELDAALEEMILLEEELG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1U0</accession>
    <name>R5V1U0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Glycosyl hydrolase family 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN566_00194</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000204">
        <property type="protein sequence ID" value="CCZ84265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00194"/>
    <dbReference type="EnsemblBacteria" id="CCZ84265">
        <property type="protein sequence ID" value="CCZ84265"/>
        <property type="gene ID" value="BN566_00194"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030248">
        <property type="term" value="F:cellulose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008965">
        <property type="entry name" value="CBM2/CBM3_carb-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001956">
        <property type="entry name" value="CBM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036966">
        <property type="entry name" value="CBM3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002105">
        <property type="entry name" value="Dockerin_1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001701">
        <property type="entry name" value="Glyco_hydro_9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00404">
        <property type="entry name" value="Dockerin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00759">
        <property type="entry name" value="Glyco_hydro_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="SSF48208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49384">
        <property type="entry name" value="SSF49384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51172">
        <property type="entry name" value="CBM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004406639" evidence="7">
        <location>
            <begin position="36"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="domain" description="CBM3" evidence="8">
        <location>
            <begin position="554"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="9">
        <location>
            <begin position="796"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="716"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="723"/>
            <end position="789"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ84265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="867" mass="95853" checksum="72CFEC05D4CF99BE" modified="2013-07-24" version="1">MKQKKHVGKRLLAGLLGAVLLQTAVSIPSGLQASAADEVEYNYAKALQYSIYFYDGNMCGTEVAENNRYQWRGNCHTYDAQVPLQPMGEDKVGTNLSQSFIDQHRDILDPDGDGYVDVSGGFHDAGDHVKFGMPENYAASTLGWGYYEFRDSYKKTGQDDHIETILRYFNDYLMKCTFRDENGEVVAHCYQVGDGDIDHAYWNSPEVDTMARPAFFLTGDKPQTDYVASAAASLAINYLNFKDTDPDYAAQSLDYAKALFQFAKSHEKQLSDNADGPKSYYNSAKWEDDYCWAAAWLFKITGDESYLQEIYPYYDYYAAPCYVYCWNDMWNGVQCILGEISEDKPAKEGEHVYPQFIEKYKEAAEKSPYEEMDCWASVAKAITTYMTGGVGTITPAGYFWLNTWGSARYNTAAQLCALVYDKYNNNGKPSEYSEWAKEQMQYLMGNNPMNRAYIVGYSENAAKYPHHRAASGLTRAEDTREQRHVLYGALVGGPDASDKHNDITADWIYNEVTIDYNAAFVGASAGLYAYFGDDSMQVTPDFPPKEENNGEEGGGNNYWVEAFAVNNPCAGGAGTTKISMKVMTDSITPRTDVTVRYFFNTKEMTNPSLVQATELYDQAATEAAPADGVISGPFQYDASYDPNIYYFEVAWDGYTIANSNKKYQCSVGLYYGDQWDASNDWSYQNLPVLTDSEMFGDGNEVKSDYICVYAGGELVGGTEPNGSTPKPVETTTSTTTATTTTTTTTTVTNATTTESEVSTTSATEITTTESKPATTTSLTEATTTESTTTATTEAGAEIYYGDVNQDGVVDLADAIVMNKALANVITLSESAGKCADCYDDGILDDKDATILMRFVIGKINDVPSKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1W4</accession>
    <name>R5V1W4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">50S ribosomal protein L23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rplW</name>
        <name evidence="6" type="ORF">BN566_00059</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">One of the early assembly proteins it binds 23S rRNA. One of the proteins that surrounds the polypeptide exit tunnel on the outside of the ribosome. Forms the main docking site for trigger factor binding to the ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the 50S ribosomal subunit. Contacts protein L29, and trigger factor when it is bound to the ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the universal ribosomal protein uL23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000241">
        <property type="protein sequence ID" value="CCZ84513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00059"/>
    <dbReference type="EnsemblBacteria" id="CCZ84513">
        <property type="protein sequence ID" value="CCZ84513"/>
        <property type="gene ID" value="BN566_00059"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01369_B">
        <property type="entry name" value="Ribosomal_L23_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_L23/L15e_core_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013025">
        <property type="entry name" value="Ribosomal_L25/23"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11620">
        <property type="entry name" value="PTHR11620"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00276">
        <property type="entry name" value="Ribosomal_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="SSF54189"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ84513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="108" mass="12070" checksum="58E1B2F08D210EE3" modified="2013-07-24" version="1">MKAPQDIILKPMITEQSVDNMQQSKYTFKVAKDANKIEIAQAAEALFHVEVVKVNTVNCTGKTKRVGRFTGKKADFKKAIITINPETTKTGKDGKKLKTSIEFFDGMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>R5V215</accession>
    <name>R5V215_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CRISPR-associated endoribonuclease Cas2</fullName>
            <ecNumber evidence="9">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">cas2</name>
        <name evidence="11" type="ORF">BN566_00268</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Functions as a ssRNA-specific endoribonuclease. Involved in the integration of spacer DNA into the CRISPR cassette.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer, forms a heterotetramer with a Cas1 homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9 10">Belongs to the CRISPR-associated endoribonuclease Cas2 protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="9"/>
    <dbReference type="EMBL" id="CBAR010000218">
        <property type="protein sequence ID" value="CCZ84355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00268"/>
    <dbReference type="EnsemblBacteria" id="CCZ84355">
        <property type="protein sequence ID" value="CCZ84355"/>
        <property type="gene ID" value="BN566_00268"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043571">
        <property type="term" value="P:maintenance of CRISPR repeat elements"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09725">
        <property type="entry name" value="Cas2_I_II_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01471">
        <property type="entry name" value="Cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021127">
        <property type="entry name" value="CRISPR_associated_Cas2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019199">
        <property type="entry name" value="Virulence_VapD/CRISPR_Cas2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34405">
        <property type="entry name" value="PTHR34405"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09827">
        <property type="entry name" value="CRISPR_Cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF032582">
        <property type="entry name" value="Cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01573">
        <property type="entry name" value="cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 10" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="5 9 10" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6 9 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 9 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 9 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 9 10" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR032582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ84355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="96" mass="11217" checksum="B2273D64EB76D7BB" modified="2013-07-24" version="1">MLVLITYDVNTETKEGRRRLQKVAKQCVNYGIRVQNSVFECVLDAAEFRKVKQILIDLIDPEKDSLRFYQLGNRYESKVEHIGVRPSLHVEEPLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5V280</accession>
    <name>R5V280_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00332</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in DNA recombination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RmuC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000225">
        <property type="protein sequence ID" value="CCZ84425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00332"/>
    <dbReference type="EnsemblBacteria" id="CCZ84425">
        <property type="protein sequence ID" value="CCZ84425"/>
        <property type="gene ID" value="BN566_00332"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003798">
        <property type="entry name" value="DNA_recombination_RmuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30563">
        <property type="entry name" value="PTHR30563"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02646">
        <property type="entry name" value="RmuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="56"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="418" mass="46656" checksum="15C3E0178BCCB98F" modified="2013-07-24" version="1">MQAAILFLLLVVICLQIFIVLVIKQSSGNVSEDSGNAAAPTQEQQKELLFHIQQNLSVLSQSLKESQQQAAAYQNQSIATLSQNLAAQMQQMEQRLRTIELTNGQGMEQVRQTIQTSLNRMQQVQADNLAEIQGMVEQKLTQTLEQKVQSAFQMVNERLEMVYRGLGEMQTLAAGVGDLKKVLSNVKTRGILGEVQLGSILQEILAPEQYAYDVTVNPNRREHVEYAVKLPGTTNGQPVWLPIDSKFPGDTYTALRNALDSGNPQQIKAAQQTLTQVIRKCAKDIHDKYICPPYTTNFAILFLPFEGLYAEVINLGMVETLQQNFQVNIAGPSTMAAMLNALQMGFQTLAIQKRSTEVWQVLQQVQTEFDRFADVLQKTQNALHSADSQLERLVGTRTRAIQRQLGAIQTQNDLHTGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5V286</accession>
    <name>R5V286_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative hexosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00506</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000266">
        <property type="protein sequence ID" value="CCZ84653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00506"/>
    <dbReference type="EnsemblBacteria" id="CCZ84653">
        <property type="protein sequence ID" value="CCZ84653"/>
        <property type="gene ID" value="BN566_00506"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="221"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="396" mass="45981" checksum="FF03BE5CB74E1D8A" modified="2013-07-24" version="1">MKRIAFVNQRYGLEVNGGSEYYTRLMAERLAKYYEVDVLTTKAVDYTTWKDWYVRDVETINGVTVRRFSVAQERAVDFDAYNAAYLAECEQGNYSRGVEKIWFEKQGPYCPECIAFIREHKDEYDAFLFVTYLYYLTVAGLPEVAEKAIFIPTAHEEPYLHFKTFEKLFQLPQAYVFLTEEERMLVRRQFKTAGIPCDVLGTGVDIPDYPPASAFRQKYRIHDPYLIYVGRIDEGKDCPTLFRYFIEFKERNPSDLKLVLMGKAVCNIPKHPDIIPLGFVSEEDKFSGLSDAISLVLPSQYESLSIAVLEAMALGVPVLVNGQCDVLKGHCTKSNGGLYYRNFFEFEGALNYLFSHPDEYQEMQENAYEYVLEHYDWDVILERFRVIIENVIEGRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5V3T6</accession>
    <name>R5V3T6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Galactose mutarotase and related enzymes</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00564</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000004">
        <property type="protein sequence ID" value="CCZ82759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00564"/>
    <dbReference type="EnsemblBacteria" id="CCZ82759">
        <property type="protein sequence ID" value="CCZ82759"/>
        <property type="gene ID" value="BN566_00564"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008183">
        <property type="entry name" value="Aldose_1/G6P_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01263">
        <property type="entry name" value="Aldose_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="SSF74650"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ82759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="335" mass="37897" checksum="6388DA85064FD51B" modified="2013-07-24" version="1">MKSTVQIINWKGMPCLQLSSGGYTAWIAPEIGSNVIRLFHEEKGLEFFRFRADNTPEAIQQSAEVWGLPTLYLPNRFADGVLKTSDAVYHLPVNEAAPYNNHIHGFLHKRKHRVMELCADESAAWAKTSYTYDETDPFFANMPIPFRADFTFRLTAEGLFYSVTFTNCSDKQMPMSLATHTTINSPFVVGAAEADSRITVPITEKWVLNERCLPTCEILPLSDYDLQYKNGTMCPVLKNIDNDMYTATAQTLNGKPFYGLTAVDTKTGKGIGYEVSENYRFWILWNDKGHNGYFCPEPMTAMIDAPNLNLPKEQTGYQELAPQETFTATQHFFAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>R5V464</accession>
    <name>R5V464_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">50S ribosomal protein L31</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rpmE</name>
        <name evidence="8" type="ORF">BN566_00711</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Binds the 23S rRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the bacterial ribosomal protein bL31 family. Type A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000022">
        <property type="protein sequence ID" value="CCZ82909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00711"/>
    <dbReference type="EnsemblBacteria" id="CCZ82909">
        <property type="protein sequence ID" value="CCZ82909"/>
        <property type="gene ID" value="BN566_00711"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00501">
        <property type="entry name" value="Ribosomal_L31_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034704">
        <property type="entry name" value="L28p-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002150">
        <property type="entry name" value="Ribosomal_L31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027491">
        <property type="entry name" value="Ribosomal_L31_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042105">
        <property type="entry name" value="Ribosomal_L31_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33280">
        <property type="entry name" value="PTHR33280"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01197">
        <property type="entry name" value="Ribosomal_L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01249">
        <property type="entry name" value="RIBOSOMALL31"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143800">
        <property type="entry name" value="SSF143800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00105">
        <property type="entry name" value="L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01143">
        <property type="entry name" value="RIBOSOMAL_L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 7" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="7">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ82909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="67" mass="7704" checksum="CDCAEB11E89015A2" modified="2013-07-24" version="1">MKKGLHPTFEKTTITCHCGNVMETMSTKKDIKVEICAKCHPFFTGKQKLVDTGGRVDRFKKRFNLDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>R5V473</accession>
    <name>R5V473_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">3-isopropylmalate dehydratase large subunit</fullName>
            <ecNumber evidence="6">4.2.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Alpha-IPM isomerase</fullName>
            <shortName evidence="6">IPMI</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Isopropylmalate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">leuC</name>
        <name evidence="8" type="ORF">BN566_00719</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate</text>
            <dbReference type="Rhea" id="RHEA:32287"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:35121"/>
            <dbReference type="EC" id="4.2.1.33"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="6">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterodimer of LeuC and LeuD.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.33" evidence="6"/>
    <dbReference type="EMBL" id="CBAR010000029">
        <property type="protein sequence ID" value="CCZ82919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00719"/>
    <dbReference type="EnsemblBacteria" id="CCZ82919">
        <property type="protein sequence ID" value="CCZ82919"/>
        <property type="gene ID" value="BN566_00719"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00071"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003861">
        <property type="term" value="F:3-isopropylmalate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01583">
        <property type="entry name" value="IPMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01027">
        <property type="entry name" value="LeuC_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011826">
        <property type="entry name" value="HAcnase/IPMdehydase_lsu_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006251">
        <property type="entry name" value="Homoacnase/IPMdehydase_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033941">
        <property type="entry name" value="IPMI_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011823">
        <property type="entry name" value="IsopropMal_deHydtase_lsu_bac"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="SSF53732"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01343">
        <property type="entry name" value="hacA_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02086">
        <property type="entry name" value="IPMI_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02083">
        <property type="entry name" value="LEU2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="3 6" id="KW-0408">Iron</keyword>
    <keyword evidence="4 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0432">Leucine biosynthesis</keyword>
    <keyword evidence="5 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aconitase" evidence="7">
        <location>
            <begin position="8"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="360"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="363"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ82919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="422" mass="45103" checksum="31F121577A070896" modified="2013-07-24" version="1">MGMTMTQKILAAHAGLDHVEAGQLILVNLDRVLGNDITSPVAIREFNKLGTDHVFDKDKVTMVMDHFAPNKDIKAATQCKMCRDFCNEKEVTHFYDVGQMGIEHALLPEKGLVVSGDVVIGADSHTCTYGALGAFSTGVGSTDMACGMAIGKAWFKVPSAIQFELKGKLNPYISGKDVILHIIGMIGVDGALYKSMEFVGEGVASLSMSDRLTMTNMAIEAGAKNGIFAVDDQTLAYVAEHSDRKPVVYTADEDAVYEEKYVIDLSEIKSTVAFPHLPGNTRTIDEVGDVKIDQVVIGSCTNGFYRDMEEAASILKGKKVAKNVRAIIIPATQSIYLKAMENGLLKTFIEAGCVVSTPTCGPCLGGHMGILAAGERAVSTTNRNFVGRMGHPTSEVYLASPAVAAASAITGRITDPKEVLAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5V4T5</accession>
    <name>R5V4T5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">N-acetylmuramoyl-L-alanine amidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00906</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000059">
        <property type="protein sequence ID" value="CCZ83119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00906"/>
    <dbReference type="EnsemblBacteria" id="CCZ83119">
        <property type="protein sequence ID" value="CCZ83119"/>
        <property type="gene ID" value="BN566_00906"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008745">
        <property type="term" value="F:N-acetylmuramoyl-L-alanine amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009253">
        <property type="term" value="P:peptidoglycan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06583">
        <property type="entry name" value="PGRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.80.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036505">
        <property type="entry name" value="Amidase/PGRP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002502">
        <property type="entry name" value="Amidase_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01510">
        <property type="entry name" value="Amidase_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00644">
        <property type="entry name" value="Ami_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55846">
        <property type="entry name" value="SSF55846"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetylmuramoyl-L-alanine amidase" evidence="1">
        <location>
            <begin position="33"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="225" mass="24976" checksum="0CE98729AD9FC601" modified="2013-07-24" version="1">MAKLVRVHYYVDNVCAWQNLPHSLSGWHAADGSGNGNRRTIAIECIMSSAYNSTNKKSEDNCAKLAAALLKQYGLDINHLYTHLPPTLSGWHAADGSGNGNRKTIAIECIMSGTYNSTDKKSEDNCARLAAALLKQYGLGISHLYTHTHWLNVRDGKSGTVDQLNTMYNRYKMCPAYILPHWAEFKKKVQSYLNAGTSTISAPSTKQIYRVRKSWADAKSQLGAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5V5H9</accession>
    <name>R5V5H9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transglutaminase-like enzymes putative cysteine proteases</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01165</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000094">
        <property type="protein sequence ID" value="CCZ83389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01165"/>
    <dbReference type="EnsemblBacteria" id="CCZ83389">
        <property type="protein sequence ID" value="CCZ83389"/>
        <property type="gene ID" value="BN566_01165"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002931">
        <property type="entry name" value="Transglutaminase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01841">
        <property type="entry name" value="Transglut_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00460">
        <property type="entry name" value="TGc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="SSF54001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="191"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="242"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="761"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="domain" description="TGc" evidence="2">
        <location>
            <begin position="606"/>
            <end position="685"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="901" mass="101309" checksum="FDB51B2754787AFC" modified="2013-07-24" version="1">MTQTEEHLTSDGIQVQDDISITIRPAAAWMRNLAAVFLAACGFIGTIAASLSMFSPIYNRTKLILVAAAIFILYSVLSILPKKLRWLTPVSLAVGTLGFFWKRNVLIVGFQFFYNHFYQQIYDTNVLYYQMDTTLVESTCTTFFLICCIVFLAAAICWFTIHRPFFPIGFIVTFFPIELGLYNGLELSLPAMALVLLYWASLLAMQLTSFRYAQEQREIGFFRKGNRFTAAYTMRTTMAERCGMIVAGLMALVFLLSWGFFTFSGVTERTSVQQKRKEIKNSLQTFDVEDLQDSLSNVGAALGLTNAMRVQKLGRKRAIDIQNEPQLQLTLDQLPDSAMYLKSFTGTTYDDNAWTALKQSDWDDQTDLVTLSETFLCYPQLYPYWFQNYQDATAMTNVDISALKWERRCYVPYASYQEQAKYEEDFVTKLDSKKNYSFTVSLHQNFSDLLQNSGVQDMYLQREIFNATDSNTQKFLAALSLPDSTDAIPVTTYFQALKEDGTYDQHAIQSMLAEQYLYRPFVYEQYTQKTETDALQAVYNALPASIQQTNLNPDFSTEDLRNGAGENLSDQLAVLESIRQFLADNATYSTSPGKTPGSRDFVNYFLMENHEGYCVHFATAGVLLARYAGIPARYCEGYVATPSDFEKAKQKKDGSYTVTLTDARAHAWCEFYVTGYGWIPFEFTPGYYGGAAEPEEGTAEATTTTTTTAAVRTEIATTEQTTEQTIGIATQTTAVVTSTPSQLGTNSRTDSGNHSSIGRTILRILLVLVLLAAVIAAPFLLRMWALQKRTEQFHAADRKKAILWMYAYWERLLETVSIRAGNESVLAFSKTAAQKLQEQQLPSDNTAPFCELVSAVNFGGATPSAEACAAAEQALQQLAEAIYQAKSPLQKLYFKYIQHLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>R5V620</accession>
    <name>R5V620_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Homoserine O-acetyltransferase</fullName>
            <shortName evidence="6">HAT</shortName>
            <ecNumber evidence="6">2.3.1.31</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Homoserine transacetylase</fullName>
            <shortName evidence="6">HTA</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">metAA</name>
        <name evidence="8" type="ORF">BN566_01313</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Transfers an acetyl group from acetyl-CoA to L-homoserine, forming acetyl-L-homoserine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>acetyl-CoA + L-homoserine = CoA + O-acetyl-L-homoserine</text>
            <dbReference type="Rhea" id="RHEA:13701"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
            <dbReference type="ChEBI" id="CHEBI:57716"/>
            <dbReference type="EC" id="2.3.1.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; O-acetyl-L-homoserine from L-homoserine: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MetA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.31" evidence="6"/>
    <dbReference type="EMBL" id="CBAR010000121">
        <property type="protein sequence ID" value="CCZ83544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01313"/>
    <dbReference type="EnsemblBacteria" id="CCZ83544">
        <property type="protein sequence ID" value="CCZ83544"/>
        <property type="gene ID" value="BN566_01313"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00074"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004414">
        <property type="term" value="F:homoserine O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008899">
        <property type="term" value="F:homoserine O-succinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019281">
        <property type="term" value="P:L-methionine biosynthetic process from homoserine via O-succinyl-L-homoserine and cystathionine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03131">
        <property type="entry name" value="GATase1_HTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00295">
        <property type="entry name" value="MetA_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005697">
        <property type="entry name" value="HST_MetA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033752">
        <property type="entry name" value="MetA_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20919">
        <property type="entry name" value="PTHR20919"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04204">
        <property type="entry name" value="HTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000450">
        <property type="entry name" value="H_ser_succinyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01001">
        <property type="entry name" value="metA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 8" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="6 7">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="235"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="237"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for acyl-CoA specificity" evidence="6">
        <location>
            <position position="111"/>
        </location>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="6">
        <location>
            <position position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000450-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="315" mass="36730" checksum="685344E69C9813C6" modified="2013-07-24" version="1">MPICIPKELPAFQTLANENIFVMDNDRASHQDIRPLRVLLLNLMPKKIETECQFLRLLSNTPIQVNVEFLQVSSHVSKNTNKMHLDAFYHTFAEIQNEYYDGCIITGAPVEHLSFADVDYWEELQQIMEWTKTHVFSTMHICWGALAGLNYHFGIPKYDLPEKMFGVFPHEVLQKNVQLLRGFDDCFYAPHSRHSEVRRADIEQVPNLKILTESPQAGVHIVANTNGRQYFITGHLEYDRMTLAEEYFRDKEKGCKIAVPAHYFPNDDPSKPPLYTWGCCANLLFANWINHCVYQLTPYDLQRLELYNWEWEAGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>R5V687</accession>
    <name>R5V687_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CTP synthase</fullName>
            <ecNumber evidence="9">6.3.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Cytidine 5'-triphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Cytidine triphosphate synthetase</fullName>
            <shortName evidence="9">CTP synthetase</shortName>
            <shortName evidence="9">CTPS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">UTP--ammonia ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">pyrG</name>
        <name evidence="12" type="ORF">BN566_00161</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>ATP + H2O + L-glutamine + UTP = ADP + CTP + 2 H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:26426"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + NH4(+) + UTP = ADP + CTP + 2 H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:16597"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>H2O + L-glutamine = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="9">Allosterically activated by GTP, when glutamine is the substrate; GTP has no effect on the reaction when ammonia is the substrate. The allosteric effector GTP functions by stabilizing the protein conformation that binds the tetrahedral intermediate(s) formed during glutamine hydrolysis. Inhibited by the product CTP, via allosteric rather than competitive inhibition.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homotetramer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="9">CTPSs have evolved a hybrid strategy for distinguishing between UTP and CTP. The overlapping regions of the product feedback inhibitory and substrate sites recognize a common feature in both compounds, the triphosphate moiety. To differentiate isosteric substrate and product pyrimidine rings, an additional pocket far from the expected kinase/ligase catalytic site, specifically recognizes the cytosine and ribose portions of the product inhibitor.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the CTP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.2" evidence="9"/>
    <dbReference type="EMBL" id="CBAR010000146">
        <property type="protein sequence ID" value="CCZ83634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00161"/>
    <dbReference type="EnsemblBacteria" id="CCZ83634">
        <property type="protein sequence ID" value="CCZ83634"/>
        <property type="gene ID" value="BN566_00161"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00159">
        <property type="reaction ID" value="UER00277"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003883">
        <property type="term" value="F:CTP synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03113">
        <property type="entry name" value="CTPS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01746">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01227">
        <property type="entry name" value="PyrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004468">
        <property type="entry name" value="CTP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017456">
        <property type="entry name" value="CTP_synthase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033828">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550">
        <property type="entry name" value="PTHR11550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06418">
        <property type="entry name" value="CTP_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00337">
        <property type="entry name" value="PyrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="4 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 9" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CTP_synth_N" evidence="11">
        <location>
            <begin position="3"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="GATase" evidence="10">
        <location>
            <begin position="302"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Amidoligase domain" evidence="9">
        <location>
            <begin position="1"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="L-glutamine binding" evidence="9">
        <location>
            <begin position="382"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; for glutamine hydrolysis" evidence="9">
        <location>
            <position position="381"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="507"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="509"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="13"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="14"/>
            <end position="19"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="148"/>
            <end position="150"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <note>allosteric inhibitor</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="188"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="224"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="354"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="405"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="462"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF06418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CCZ83634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="539" mass="59851" checksum="40BAEF581993B06B" modified="2013-07-24" version="1">MPKYVFVTGGVVSGLGKGITAASLGRLLKARGYRVTIQKFDPYINVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDENLSINSNVTTGKIYWTVLNKERRGDYLGGTVQVIPHITNEIKERIYRVGKSSDTDVVITEIGGTVGDIESTPFLEAIRQFVAEVGRQNAMYIHVTLVPYISGSNELKSKPTQHSVKELLSIGIQPNVIVCRTEQEIPKEMAKKISLFCNVREEDIIQNMTAPSLYDVPMMLEQEGLAACVCHHLGLENRTPDLTEWIQMTERQKQATEQVTIGLVGKYVALPDAYLSVAEALRHGGIPNDVKVEILWIDSEKITGATVAETLKDCDGIIVPGGFGDRGIEGMITAIEYARENQIPMFGICLGMQMAVVEFARHCAGLPDANSAEFLPEGTQNVIDIMEDQKDITNLGGTMRLGLYPCKLTDGAKVRSIYGEELIYERHRHRFELNNAFRRQLTESGLQLAGISPDGKLVEIVELPSHPWFIGVQFHPEFKSRPNKPHKLFTSFIAAALQQKRKAEAES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>R5V6I4</accession>
    <name>R5V6I4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Quinolinate synthase</fullName>
            <ecNumber evidence="3">2.5.1.72</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN566_01469</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from iminoaspartate: step 1/1.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.72" evidence="3"/>
    <dbReference type="EMBL" id="CBAR010000159">
        <property type="protein sequence ID" value="CCZ83734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01469"/>
    <dbReference type="EnsemblBacteria" id="CCZ83734">
        <property type="protein sequence ID" value="CCZ83734"/>
        <property type="gene ID" value="BN566_01469"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00327"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008987">
        <property type="term" value="F:quinolinate synthetase A activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10800">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003473">
        <property type="entry name" value="NadA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036094">
        <property type="entry name" value="NadA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30573">
        <property type="entry name" value="PTHR30573"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02445">
        <property type="entry name" value="NadA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142754">
        <property type="entry name" value="SSF142754"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00550">
        <property type="entry name" value="nadA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ83734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="305" mass="34048" checksum="A44DC717CB9AB6BE" modified="2013-07-24" version="1">MTTKEMQEAILKWKKEQDCCILAHCYQTHDILEVADFVGDSFGLAQKAAQTNCKRVMMCGVRFMAETVKILSPEKQVYLPKAEAGCPMAEQLDVEMLQQMKEIYPEHCVVAYINTTAALKTLCDVCVTSSSAVDIIRKIPNKKILFIPDCNLGDYVAKQIPEKEFQLMHGGCPTHVRITVKDVEKTRAAHPDAELLVHPECQPAVTAQADYVGSTTGIMNYAAKSDKKSFIIGTENSIVQHLGMQFPDKLFYPLSKDCVCHNMKITTLADVLHCLQGVDGEEIQMPEDVMQKAKVCIEEMLRLGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>R5V6J5</accession>
    <name>R5V6J5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">rRNA methylase putative group 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01479</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000161">
        <property type="protein sequence ID" value="CCZ83744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01479"/>
    <dbReference type="EnsemblBacteria" id="CCZ83744">
        <property type="protein sequence ID" value="CCZ83744"/>
        <property type="gene ID" value="BN566_01479"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="L30e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004441">
        <property type="entry name" value="rRNA_MeTrfase_TrmH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013123">
        <property type="entry name" value="SpoU_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46429">
        <property type="entry name" value="PTHR46429"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08032">
        <property type="entry name" value="SpoU_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00967">
        <property type="entry name" value="SpoU_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="SSF55315"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00186">
        <property type="entry name" value="rRNA_methyl_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SpoU_sub_bind" evidence="2">
        <location>
            <begin position="20"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="261" mass="28089" checksum="4A35584B45D7015A" modified="2013-07-24" version="1">MKPERQPRNRTAAPQEEENRIAGRNPVMEALKSGTPLDTIYMSGKQGILGQIAQLAADQGIPVKIVSDARLSQMTRQTHQGVVADGACATYASIAEILEYAAEKGEKPLLLICDEIQDPHNLGAILRTAEAAGFHGIILPKRRSASLNATVAKTSAGAVSWVKVARVPNLVAAMEELKQNGIWIYGTDAAGTDYTQTDFRDGTAFVIGSEGNGMGRLVREHCDQLLSLPMYGKVNSLNASVAASIFMYEAVRQRRNPSGKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>R5V717</accession>
    <name>R5V717_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4 5">30S ribosomal protein S12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpsL</name>
        <name evidence="6" type="ORF">BN566_01623</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 5">Interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone. Located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side and probably holding the rRNA structure together. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit.</text>
    </comment>
    <comment type="function">
        <text evidence="1 4 5">With S4 and S5 plays an important role in translational accuracy.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 5">Part of the 30S ribosomal subunit. Contacts proteins S8 and S17. May interact with IF1 in the 30S initiation complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the universal ribosomal protein uS12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000171">
        <property type="protein sequence ID" value="CCZ83894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01623"/>
    <dbReference type="EnsemblBacteria" id="CCZ83894">
        <property type="protein sequence ID" value="CCZ83894"/>
        <property type="gene ID" value="BN566_01623"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03368">
        <property type="entry name" value="Ribosomal_S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00403_B">
        <property type="entry name" value="Ribosomal_S12_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006032">
        <property type="entry name" value="Ribosomal_S12/S23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005679">
        <property type="entry name" value="Ribosomal_S12_bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11652">
        <property type="entry name" value="PTHR11652"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00164">
        <property type="entry name" value="Ribosom_S12_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01034">
        <property type="entry name" value="RIBOSOMALS12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00981">
        <property type="entry name" value="rpsL_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00055">
        <property type="entry name" value="RIBOSOMAL_S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 5" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="4 5" id="KW-0820">tRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ83894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="140" mass="15363" checksum="CB4D86C8A6C8A6F0" modified="2013-07-24" version="1">MPTFNQLVRKGRKVQAVKSTAPALQKGYNAKKKVTTDQSSPQKRGVCTAVRTATPKKPNSAMRKIARVKLTNGYEVTSYIPGIGHNLQEHSVVLIRGGRVKDLPGVRYHIIRGTLDAQGVANRMQARSKYGAKRPKAGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7F6</accession>
    <name>R5V7F6_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Histidinol dehydrogenase</fullName>
            <shortName evidence="4">HDH</shortName>
            <ecNumber evidence="4">1.1.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hisD</name>
        <name evidence="11" type="ORF">BN566_01728</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the sequential NAD-dependent oxidations of L-histidinol to L-histidinaldehyde and then to L-histidine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>H2O + L-histidinol + 2 NAD(+) = 3 H(+) + L-histidine + 2 NADH</text>
            <dbReference type="Rhea" id="RHEA:20641"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:57699"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4 9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="4 9">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 9/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 5 10">Belongs to the histidinol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.23" evidence="4"/>
    <dbReference type="EMBL" id="CBAR010000178">
        <property type="protein sequence ID" value="CCZ83999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01728"/>
    <dbReference type="EnsemblBacteria" id="CCZ83999">
        <property type="protein sequence ID" value="CCZ83999"/>
        <property type="gene ID" value="BN566_01728"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00014"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004399">
        <property type="term" value="F:histidinol dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06572">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01024">
        <property type="entry name" value="HisD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001692">
        <property type="entry name" value="Histidinol_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022695">
        <property type="entry name" value="Histidinol_DH_monofunct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012131">
        <property type="entry name" value="Hstdl_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21256">
        <property type="entry name" value="PTHR21256"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00815">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000099">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00083">
        <property type="entry name" value="HOLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00069">
        <property type="entry name" value="hisD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00611">
        <property type="entry name" value="HISOL_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="1 4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 7" id="KW-0520">NAD</keyword>
    <keyword evidence="3 4 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4 9" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Proton acceptor" evidence="4 6">
        <location>
            <position position="329"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4 6">
        <location>
            <position position="330"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4 7">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 7">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 7">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 8">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 8 9">
        <location>
            <position position="261"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4 8 9">
        <location>
            <position position="264"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4 8">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 8 9">
        <location>
            <position position="363"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4 8">
        <location>
            <position position="417"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 8 9">
        <location>
            <position position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR000099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000099-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000099-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000099-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000099-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ83999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="434" mass="47056" checksum="F49DFD4AAAFB8596" modified="2013-07-24" version="1">MITMKQINASVAEEREAFFQLLDERTGETNQRVTETVQEILKTVKAGGDDAVKNYTLKFDGSLPEYYEVPREVINDALTDADQAFVDAMLNAIENVRDFHQRQVEQGFIHPMANGVILGQRVRGLERVGLYVPGGTAAYPSSVIMNAVPAKIAGVKEIIMVTPPRKDGTPNPDILIAAALCGVDRIFLSGGAQAIAALAYGTKEIPKVDKIVGPGNIYVATAKKLLYGIVDIDMIAGPSEVLVMADETANPAFAAADLMSQAEHDVMASSILVTTSEKLAEQVQAEINRQVQTLERKEIIEKSMNTYGAILLCNSREEATALANRVAPEHLEVLMENPMELLGALDNAGSVFLGQYASEPLGDYYAGPNHVLPTSGTARFFSPLGVASFTKRSSYTYYTKDALRAAKDDIVLIANREGLTAHANAITVRFEDEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7P0</accession>
    <name>R5V7P0_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">7-carboxy-7-deazaguanine synthase</fullName>
            <shortName evidence="5">CDG synthase</shortName>
            <ecNumber evidence="5">4.3.99.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Queuosine biosynthesis protein QueE</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">queE</name>
        <name evidence="7" type="ORF">BN566_01845</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the complex heterocyclic radical-mediated conversion of 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) to 7-carboxy-7-deazaguanine (CDG), a step common to the biosynthetic pathways of all 7-deazapurine-containing compounds.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>6-carboxy-5,6,7,8-tetrahydropterin + H(+) = 7-carboxy-7-deazaguanine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:27974"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:61032"/>
            <dbReference type="ChEBI" id="CHEBI:61036"/>
            <dbReference type="EC" id="4.3.99.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </cofactor>
        <text evidence="5">Binds 1 S-adenosyl-L-methionine per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="5">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the radical SAM superfamily. 7-carboxy-7-deazaguanine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.99.3" evidence="5"/>
    <dbReference type="EMBL" id="CBAR010000187">
        <property type="protein sequence ID" value="CCZ84119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01845"/>
    <dbReference type="EnsemblBacteria" id="CCZ84119">
        <property type="protein sequence ID" value="CCZ84119"/>
        <property type="gene ID" value="BN566_01845"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00391"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016840">
        <property type="term" value="F:carbon-nitrogen lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904047">
        <property type="term" value="F:S-adenosyl-L-methionine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008616">
        <property type="term" value="P:queuosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00917">
        <property type="entry name" value="QueE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023868">
        <property type="entry name" value="7-CO-7-deazaGua_synth_put_Clo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024924">
        <property type="entry name" value="7-CO-7-deazaguanine_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000370">
        <property type="entry name" value="QueE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03963">
        <property type="entry name" value="rSAM_QueE_Clost"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3 5" id="KW-0408">Iron</keyword>
    <keyword evidence="4 5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0671">Queuosine biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="6">
        <location>
            <begin position="20"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="14"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="5">
        <location>
            <begin position="39"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ84119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="223" mass="24961" checksum="9137A3846BE6E40A" modified="2013-07-24" version="1">MSVTYRVAEQFVSINGEGTRAGELAVFVRLCGCNLQCSYCDTQWANQPDVSYQLQTTEEIIQAILETGVRNVTLTGGEPLLTPNVAELIAAITAQPTLSLEIETNGSVPLQSFAAIQPRPIFTMDYKLPSSGMEHKMCLENMQLLQPQDTVKLVSGSLEDLERGAALIQQYDLTHRCHVYFSPVFGSIEPQEMVQFLIERKLNGVRLQLQLHKYIWDPQERGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7R1</accession>
    <name>R5V7R1_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA(Ile)-lysidine synthase</fullName>
            <ecNumber evidence="6">6.3.4.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">tRNA(Ile)-2-lysyl-cytidine synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">tRNA(Ile)-lysidine synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">tilS</name>
        <name evidence="9" type="ORF">BN566_01869</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>ATP + cytidine(34) in tRNA(Ile2) + L-lysine = AMP + diphosphate + H(+) + lysidine(34) in tRNA(Ile2)</text>
            <dbReference type="Rhea" id="RHEA:43744"/>
            <dbReference type="Rhea" id="RHEA-COMP:10625"/>
            <dbReference type="Rhea" id="RHEA-COMP:10670"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:83665"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.19"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="6">The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the tRNA(Ile)-lysidine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.19" evidence="6"/>
    <dbReference type="EMBL" id="CBAR010000192">
        <property type="protein sequence ID" value="CCZ84144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01869"/>
    <dbReference type="EnsemblBacteria" id="CCZ84144">
        <property type="protein sequence ID" value="CCZ84144"/>
        <property type="gene ID" value="BN566_01869"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01992">
        <property type="entry name" value="PP-ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01161">
        <property type="entry name" value="tRNA_Ile_lys_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011063">
        <property type="entry name" value="TilS/TtcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012094">
        <property type="entry name" value="tRNA_Ile_lys_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012795">
        <property type="entry name" value="tRNA_Ile_lys_synt_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43033">
        <property type="entry name" value="PTHR43033"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01171">
        <property type="entry name" value="ATP_bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02432">
        <property type="entry name" value="lysidine_TilS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="ATP_bind_3" evidence="8">
        <location>
            <begin position="27"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="456"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="470"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="32"/>
            <end position="37"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ84144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="487" mass="54929" checksum="6CE58839C20A49ED" modified="2013-07-24" version="1">MKHKETLPEQVQAFLQEQQMVQAGDTVCCALSGGADSVCLLRCLLELAEPLHLTITAVHVNHQLRGAESERDAAFCEALCTRWNVPLHMVRCDVNAYAEQHHASIETAARACRYAAFSAIPANHIATAHTASDNLETMLHRLIRGSGLRGLAGIPAVRERYIRPLLTVTRADVEAYLQTLGQDYVTDSTNETDAYTRNQIRHQLIPILKQLNPSLEQTSIGMLQVLRSEQDYLRQQTEYCFQQHMTAAGTLSGLLEEHPAMQQRCIIQLLEQFQISYQADRIARLQRLLQTGGKCNLNADYDAVAKNGSLSIQKRVTLPEPVKPKILHEGENQIFSGFLVKARMVSWENMRNPLIVHKKLSKIHLDYDKIRGVILLRGRQYGDHVQFAGHRTVASVKKQIQKQVPPERRQNLHFLADEAGTIFAEFLGVAARVAPTAETTHVLELEMIQEQTRLEQLPESSLGLPRKTENGETSLKEWSEQFDTKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7U0</accession>
    <name>R5V7U0_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Peptidase_S11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BN566_01904</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the peptidase S11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01904"/>
    <dbReference type="EnsemblBacteria" id="CCZ84179">
        <property type="protein sequence ID" value="CCZ84179"/>
        <property type="gene ID" value="BN566_01904"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009002">
        <property type="term" value="F:serine-type D-Ala-D-Ala carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018044">
        <property type="entry name" value="Peptidase_S11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001967">
        <property type="entry name" value="Peptidase_S11_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00768">
        <property type="entry name" value="Peptidase_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00725">
        <property type="entry name" value="DADACBPTASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0133">Cell shape</keyword>
    <keyword evidence="5" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S11" evidence="11">
        <location>
            <begin position="88"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="352"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="356"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="372"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="6">
        <location>
            <position position="122"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="125"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="182"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR618044-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR618044-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CCZ84179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="411" mass="44894" checksum="937756514545E7B0" modified="2013-07-24" version="1">MVFRNKYTGKINWIPLLGTIAGGLFLIVLLTVILSEVFRGDPVQSTATSSGSGSMIAQPITDISYSEASDGSMVIDNYPEYAKDEKKLTENNIYSKYAVLLDVKNNQILADRNCEQKMYPASMTKLMSLLVAVENIKDFNDTYTITYELIAPLIEQEAARAGFESGETVSITDLLYGMALPSGADATMAIVDYVSGNEETFVALMNQKAKALGMNHTHFTNAVGLHDENHYSTALDIAILMKAAMENPTCRQILGSVEYRTTSTQQHPNGMILLSTMYKRMYGNEVKNMTIIGGKTGFTDQAMQCLASYATAVDGNEYVVVTAYAPTEMNPVFDSFAMYGLVNGGYEMPTHLEKTTYPPTEATTTDSSDDSDSTETASDAETELDSSSEDLYGNGDTEATDPEESSSELYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7Y6</accession>
    <name>R5V7Y6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Carbohydrate ABC transporter ATP-binding protein CUT1 family (TC 3.A.1.1.-)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_00178</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000195">
        <property type="protein sequence ID" value="CCZ84239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00178"/>
    <dbReference type="EnsemblBacteria" id="CCZ84239">
        <property type="protein sequence ID" value="CCZ84239"/>
        <property type="gene ID" value="BN566_00178"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008643">
        <property type="term" value="P:carbohydrate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03301">
        <property type="entry name" value="ABC_MalK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015855">
        <property type="entry name" value="ABC_transpr_MalK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040582">
        <property type="entry name" value="OB_MalK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17912">
        <property type="entry name" value="OB_MalK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="SSF50331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="4"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="37"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ84239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="371" mass="41722" checksum="15BF42E0E53778E9" modified="2013-07-24" version="1">MASLSLRHIYKIYPGNVTAVKDVNIEIKDKEFIVLVGPSGCGKSTTLRMIAGLEEISKGELYIGDRLVNDIAPKDRDIAMVFQNYALYPHMTVYENMAFGLKLRKVPKDEIDRKVKEAAKILDLSHLLDRKPKAMSGGQRQRVAMGRAIVRNPQVFLLDEPLSNLDAKLRAQMRTEISKIHKKLGTTFIYVTHDQTEAMTLGDRIVVMKDGIIQQIDTPQNLYERPGNKFVAGFLGSPQMNFIDGVLKKKNNGYCVQFAKKYELAVPAAKEDKKLANYVDKEVILGIRPEAIHDDEMFLSSATTGVIECDVDITEMMGAENFLYLDCQGTPLTARVDPRSTAKQGDKNLRVALDPNRMHLFDKETEETIIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5V836</accession>
    <name>R5V836_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_00033</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000208">
        <property type="protein sequence ID" value="CCZ84294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00033"/>
    <dbReference type="EnsemblBacteria" id="CCZ84294">
        <property type="protein sequence ID" value="CCZ84294"/>
        <property type="gene ID" value="BN566_00033"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026906">
        <property type="entry name" value="LRR_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13306">
        <property type="entry name" value="LRR_5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004406800" evidence="4">
        <location>
            <begin position="27"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="444"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="483"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="483"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="515"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ84294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="554" mass="59412" checksum="E5736B7953878C1F" modified="2013-07-24" version="1">MKQWTQSVLGCMTAGLLMITSFPVFAADSSSDPSGKSQPVTEGSTFDDGTLTYTILKNMQVSVTGCITTATNISIMPKIDGYTVVSIGDQAFAGCSALQSVTMPNGITEIGDGAFQSCTSLQSVTLSNSITEIPDGAFLSCSALTDIQLGDQITKIGRMAFAYCSSLTDMEIPDSVTEFGEQTFMGCSSLESITLPETLETLSAYMFQNCSALESFSIPDTMTDLGYLAFVGCRNLKTIDISANHPTYQLQDDVLYSKDGTELFLYPAGKTETSFTVPDGVKTISDGAFFAAPLQSVTLPEGLEGIGSGAFDYCTSLTNITIPESVTVIQDHAFSDCESLSSVLFAGDEEATDNALQIGSYAFFCCEQLMDVTLPKRVTQIGDFAFGVTEQQKVNADGSTSDETENIAVSGFLLTGYEGAAAKYISSSRSNGIRINFKSLQIPWVKIVSISLGCTAGLVLIFLLVRIIKKKRLSAADKEALEAAEQERKIPLSQREPDPEPEEPEEPDYVSILEDMSHSQMTHQFGHGTLPQESETDSNAESTESSETTSKSAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5V884</accession>
    <name>R5V884_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00252</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000216">
        <property type="protein sequence ID" value="CCZ84339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00252"/>
    <dbReference type="EnsemblBacteria" id="CCZ84339">
        <property type="protein sequence ID" value="CCZ84339"/>
        <property type="gene ID" value="BN566_00252"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="62" mass="7053" checksum="E256D6423359A3DC" modified="2013-07-24" version="1">MNKKGEIMKQKRISFGQGKGSLTHNNREFVADNVDPLRTPQNITFVRQPIGEAYDQLFAEST</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5V8U9</accession>
    <name>R5V8U9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">D-3-phosphoglycerate dehydrogenase</fullName>
            <ecNumber evidence="6">1.1.1.399</ecNumber>
            <ecNumber evidence="7">1.1.1.95</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">2-oxoglutarate reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN566_00403</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the reversible oxidation of 3-phospho-D-glycerate to 3-phosphonooxypyruvate, the first step of the phosphorylated L-serine biosynthesis pathway. Also catalyzes the reversible oxidation of 2-hydroxyglutarate to 2-oxoglutarate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>(2R)-3-phosphoglycerate + NAD(+) = 3-phosphooxypyruvate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:12641"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18110"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="EC" id="1.1.1.95"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(R)-2-hydroxyglutarate + NAD(+) = 2-oxoglutarate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:49612"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15801"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.399"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 12">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.399" evidence="6"/>
    <dbReference type="EC" id="1.1.1.95" evidence="7"/>
    <dbReference type="EMBL" id="CBAR010000240">
        <property type="protein sequence ID" value="CCZ84504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00403"/>
    <dbReference type="EnsemblBacteria" id="CCZ84504">
        <property type="protein sequence ID" value="CCZ84504"/>
        <property type="gene ID" value="BN566_00403"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00135">
        <property type="reaction ID" value="UER00196"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004617">
        <property type="term" value="F:phosphoglycerate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0718">Serine biosynthesis</keyword>
    <feature type="domain" description="ACT" evidence="13">
        <location>
            <begin position="317"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00021582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CCZ84504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="387" mass="40748" checksum="E0F1B2938BB8C6DE" modified="2013-07-24" version="1">MYTIQTLNKIAAAGTKRFDTAAYTVTDTAENPDAIMVRSAAMHDMTFGSNLLAIARAGAGTNNIPVDRCAEEGICVFNTPGANANAVKELVLTGLLLSSRKIPQAIAWAHSLEHEEGIAKLVEKGKSQFAGPEIKGKTLGIIGLGAIGALVANAAIALGMQVVGTDPFLSVGAALRLNPAVKIVKDSDGVFAAADYLTIHVPFNNDTKGMINAETIAKMKDGVRVLNYARGELVNDDDMLAALASGKVTCYCTDFPNEKTCNAEGIIATPHLGASTPESEENCASMAAEELISYLETGNIRNSVNLPNAEMQAIGNKVCILHKNVPNMIAAVTAVAGTAGVNIENMVNASRKSYAYTMLDIPETVTDAMLDQIRAIDGVIRVRLIAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>R5V8Y4</accession>
    <name>R5V8Y4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Alanine--tRNA ligase</fullName>
            <ecNumber evidence="11">6.1.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Alanyl-tRNA synthetase</fullName>
            <shortName evidence="11">AlaRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">alaS</name>
        <name evidence="14" type="ORF">BN566_00415</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 11">Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala)</text>
            <dbReference type="Rhea" id="RHEA:12540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:9923"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78497"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.7" evidence="11"/>
    <dbReference type="EMBL" id="CBAR010000244">
        <property type="protein sequence ID" value="CCZ84554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00415"/>
    <dbReference type="EnsemblBacteria" id="CCZ84554">
        <property type="protein sequence ID" value="CCZ84554"/>
        <property type="gene ID" value="BN566_00415"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004813">
        <property type="term" value="F:alanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006419">
        <property type="term" value="P:alanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00036_B">
        <property type="entry name" value="Ala_tRNA_synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002318">
        <property type="entry name" value="Ala-tRNA-lgiase_IIc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018162">
        <property type="entry name" value="Ala-tRNA-ligase_IIc_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018165">
        <property type="entry name" value="Ala-tRNA-synth_IIc_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018164">
        <property type="entry name" value="Ala-tRNA-synth_IIc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023033">
        <property type="entry name" value="Ala_tRNA_ligase_euk/bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01411">
        <property type="entry name" value="tRNA-synt_2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00980">
        <property type="entry name" value="TRNASYNTHALA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101353">
        <property type="entry name" value="SSF101353"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="SSF55186"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00344">
        <property type="entry name" value="alaS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50860">
        <property type="entry name" value="AA_TRNA_LIGASE_II_ALA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 11 14" id="KW-0436">Ligase</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 11" id="KW-0820">tRNA-binding</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II_ALA" evidence="13">
        <location>
            <begin position="4"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="731"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="562"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="566"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="664"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="668"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CCZ84554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="878" mass="96563" checksum="ADB378D44E1F8A98" modified="2013-07-24" version="1">MKWQGLNELREKYLSFFESKNHTRLPSFSLIPQGDKSLLLINSGMAPLKKYFLGQAVPPNVRVTTCQKCIRTPDIERVGQTARHGTYFEMLGNFSFGDYFKKQAIEWAWEFFTKELEMPVDKLYVSVFENDDEAYDLWTKHIGVEPSHMKRLGREDNFWEHGSGPCGPCSEIYFDRGEEKGCGKPTCGVGCDCDRYVEVWNLVFSQFNSDGNGHYTEMEHKNIDTGMGLERLACIMQGVDNLFEVDTVQNIMKHVCKIANVAYHTDDKTDVALRVITDHIRSTTFMIGDGVMPSNAGRGYVLRRLLRRAARYGRLIGIEKPFLYEVCDTVIAENRVAYPELEEKKAYIRKIVQVEEETFAKTVDNGMAMLNQILDQIDQKEISGEDAFKLSDTYGFPIDLTMEIAAEQGVSVDREGFDALVKAQREKSQADHLAKAGSSWAGNSIRIAGEKTVFTGYEHLTETDAKIIAIYKEQDAVDVAVEGDTVVIVLDKTPFYAESGGQVGDCGTISTASCTATVSSTSKNEDGHYLHIATVEDGDLHVGDVVTAAVAEEVRRATMQNHTAAHLLQAALREVLGDHVHQAGQLVTPQSCRFDFSHFSAMTKEEIQKVEQLVNEKILAAIPVVTESLPIAEARKRGAMALFGEKYGDVVRVVSVSDFSVEFCGGTHVDNTAKLGLFRIVSENSVASGVRRIEAVTGTGVLRLLDAAQLEMQQAAQALKLGNVSELAERCTSVMAELKEKDRALESLNQKISDAQIEAAFNNVNEVKGVKVISAMLNGVNPQMLRKMGDRIKAMDEPYIAVLAGVQDGKGSLLCACSKAAIAHGAHAGQIVQRVAAYTGGKGGGKAEQAMAGVGQTYMIDEALMAAENIIRNLISEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>R5V8Z3</accession>
    <name>R5V8Z3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Sigma-70 region 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_00425</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000246">
        <property type="protein sequence ID" value="CCZ84564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84564">
        <property type="protein sequence ID" value="CCZ84564"/>
        <property type="gene ID" value="BN566_00425"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="3">
        <location>
            <begin position="15"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="4">
        <location>
            <begin position="106"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ84564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="180" mass="20907" checksum="75249BC2E9A1D55C" modified="2013-07-24" version="1">MQSVNPLDEAAFTELYVQHVDAVYRICYSFLKNRTDAEDAVQETFLRLMQSQKPLENDAHLRGWLVVTASNVCKNMLHSWLWQKRQEVEDWEEVLKSDNQTPEETAVLKALMQLPEKYKTVLYLYYYEEYSCKEIAEMMQKKESTVRSLLKRGREALQKKIGGDGYATGLESHLGQSQSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5VGN6</accession>
    <name>R5VGN6_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Biotin transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the BioY family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000001">
        <property type="protein sequence ID" value="CCZ82746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00001"/>
    <dbReference type="EnsemblBacteria" id="CCZ82746">
        <property type="protein sequence ID" value="CCZ82746"/>
        <property type="gene ID" value="BN566_00001"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015225">
        <property type="term" value="F:biotin transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003784">
        <property type="entry name" value="BioY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34295">
        <property type="entry name" value="PTHR34295"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02632">
        <property type="entry name" value="BioY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016661">
        <property type="entry name" value="BioY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2 3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="56"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="113"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="147"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR016661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ82746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="190" mass="20201" checksum="3C957AFDF8CD0DD6" modified="2013-07-24" version="1">MSSSSIRSLCQIALFAAIIAVCSQIQIPLTVPFTMQTFAVFSALAILGGKNGTISILIYIALGAIGVPVFAGFSGGIGVLFGTTGGYILGFLFSGLLYWLITHFLGTKLPVMIAAMALGLIVCYTFGTVWFIQVYTSKVESIGLMAALGWCVFPFILPDCVKIALAILIAKRLPKRFVYQSVATKKDAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>R5VH06</accession>
    <name>R5VH06_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">RNA polymerase ECF subfamily sigma factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_00656</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000012">
        <property type="protein sequence ID" value="CCZ82851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82851">
        <property type="protein sequence ID" value="CCZ82851"/>
        <property type="gene ID" value="BN566_00656"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="3">
        <location>
            <begin position="23"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="4">
        <location>
            <begin position="113"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ82851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="177" mass="20343" checksum="ABC4027D06B91163" modified="2013-07-24" version="1">MLQISQSISPEEAVQPMEIQQVIQVYQQLIYGIALTGTGGNRADAEDVFQETFLAYFQANKTFREEEHRKAWLIRTACNYCKKIMGSTYRKRTVPLTDTTPLPVLFDLPEDNQLFAALCSLKPMYRNVLYLYYFAGYRTEEIAGLLGKRAGTIRMQLLRGREQLRTQLQEEEGTVDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5VHT2</accession>
    <name>R5VHT2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00849</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000047">
        <property type="protein sequence ID" value="CCZ83061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00849"/>
    <dbReference type="EnsemblBacteria" id="CCZ83061">
        <property type="protein sequence ID" value="CCZ83061"/>
        <property type="gene ID" value="BN566_00849"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008841">
        <property type="entry name" value="Siphovirus-type_tail"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05709">
        <property type="entry name" value="Sipho_tail"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="205" mass="22873" checksum="52B89F3B55551C0D" modified="2013-07-24" version="1">MKTAQYLKVYYRTVGIDVYTEGYVESCTVTNFSELVNGQISIICPDPYWYSMQPIYAYSQSVFGAFHFPFPESDEPFPLGVYSTDKTLSIFNSGEEVGILITLEAASGEDVPNPVTTAVALYDDDTSTYFQLRLDILPGDKIIINTKQGQKSVTLVRDGVTTNIINCMTSGSTWFTLRKGLNRYRLSAPKYITATIQHTDAYLGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>R5VHY6</accession>
    <name>R5VHY6_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Bifunctional protein GlmU</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="15">UDP-N-acetylglucosamine pyrophosphorylase</fullName>
                <ecNumber evidence="15">2.7.7.23</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="15">N-acetylglucosamine-1-phosphate uridyltransferase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="15">Glucosamine-1-phosphate N-acetyltransferase</fullName>
                <ecNumber evidence="15">2.3.1.157</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="15" type="primary">glmU</name>
        <name evidence="17" type="ORF">BN566_00117</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc). The C-terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N-acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5-triphosphate), a reaction catalyzed by the N-terminal domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>H(+) + N-acetyl-alpha-D-glucosamine 1-phosphate + UTP = diphosphate + UDP-N-acetyl-alpha-D-glucosamine</text>
            <dbReference type="Rhea" id="RHEA:13509"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
            <dbReference type="EC" id="2.7.7.23"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-acetyl-alpha-D-glucosamine 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:13725"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
            <dbReference type="ChEBI" id="CHEBI:58516"/>
            <dbReference type="EC" id="2.3.1.157"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="15">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="15">Bacterial outer membrane biogenesis; LPS lipid A biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="15">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route II): step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="15">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">In the C-terminal section; belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">In the N-terminal section; belongs to the N-acetylglucosamine-1-phosphate uridyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.23" evidence="15"/>
    <dbReference type="EC" id="2.3.1.157" evidence="15"/>
    <dbReference type="EMBL" id="CBAR010000068">
        <property type="protein sequence ID" value="CCZ83156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00117"/>
    <dbReference type="EnsemblBacteria" id="CCZ83156">
        <property type="protein sequence ID" value="CCZ83156"/>
        <property type="gene ID" value="BN566_00117"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00113">
        <property type="reaction ID" value="UER00532"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00973"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019134">
        <property type="term" value="F:glucosamine-1-phosphate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003977">
        <property type="term" value="F:UDP-N-acetylglucosamine diphosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009245">
        <property type="term" value="P:lipid A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03353">
        <property type="entry name" value="LbH_GlmU_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01631">
        <property type="entry name" value="GlmU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005882">
        <property type="entry name" value="Bifunctional_GlmU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038009">
        <property type="entry name" value="GlmU_C_LbH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01173">
        <property type="entry name" value="glmU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 15" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="10 15" id="KW-0133">Cell shape</keyword>
    <keyword evidence="14 15" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="4 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 15" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="6 15" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11 15" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 15" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NTP_transferase" evidence="16">
        <location>
            <begin position="4"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyrophosphorylase" evidence="15">
        <location>
            <begin position="1"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="UDP-GlcNAc binding" evidence="15">
        <location>
            <begin position="76"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Linker" evidence="15">
        <location>
            <begin position="229"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-acetyltransferase" evidence="15">
        <location>
            <begin position="250"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Acetyl-CoA binding" evidence="15">
        <location>
            <begin position="384"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="361"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="226"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="349"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="364"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="375"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="421"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="438"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CCZ83156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="464" mass="50279" checksum="17AFCEF966390870" modified="2013-07-24" version="1">MDQVVILAGGHGSRMKAAIPKPMLEVLGVPMLGWVVQACEAADLRNICVVTGYKSQFIEHYLNNKYKTVLQAERLGTGHAVMQAVPVLEENPDGNTLVLCGDAPFMDSETIQQAYQLHTAQGNAVTVITAKLDNPFGYGRILRSETGIAAIVEEKDATDAQRQIQEVNSGAYWFRTADLLTLLKGLSQSNVQGEYYLTDTIGLALEQGKKAGAYLTDNTDVVLGANTRKDLLHLNEVARWKVLEKHMENGVNFINTDGIIIGKDVEIGAETTILPGTQLKGRTKIGARSTIGPNCVIENCVVGEYVILNYVQAYESVIEDSVKIGPFVHIRPNSHIRSGVKIGDFVEVKNSVVGESTAIAHLTYVGDSDVGKKVNFGCGTVTVNYDGIKKNRCVIGDNCFIGCNTNLIAPVKLGKGAYTAAGSTVTKDVPDYALAVERADLKVKEGYSLKKLKSKLFPEDKNKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5VI38</accession>
    <name>R5VI38_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_01004</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000075">
        <property type="protein sequence ID" value="CCZ83221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83221">
        <property type="protein sequence ID" value="CCZ83221"/>
        <property type="gene ID" value="BN566_01004"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006480">
        <property type="entry name" value="Phage_holin_4_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05105">
        <property type="entry name" value="Phage_holin_4_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01593">
        <property type="entry name" value="holin_tox_secr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ83221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="136" mass="14499" checksum="273F1331BD8F98BF" modified="2013-07-24" version="1">MKEWICAAAGTVGGLIAGLFGGWDAAIRALLIFMAIDYLMGLACAGIFKKSPKTESGGLQSKVGWKGLCRKVATLALVVVAVQVDAVLHTSYVRDGVCIAFMANELISMVENVGLMGVQFPEPLRKAIDLLQKKDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5VI51</accession>
    <name>R5VI51_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">zinc_ribbon_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01017</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000081">
        <property type="protein sequence ID" value="CCZ83236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01017"/>
    <dbReference type="EnsemblBacteria" id="CCZ83236">
        <property type="protein sequence ID" value="CCZ83236"/>
        <property type="gene ID" value="BN566_01017"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026870">
        <property type="entry name" value="Zinc_ribbon_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13240">
        <property type="entry name" value="zinc_ribbon_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="zinc_ribbon_2" evidence="3">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="113"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="68"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="121"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="159" mass="17684" checksum="79B9CFB28E2F3102" modified="2013-07-24" version="1">MEVKKMFEKAGDSVSKFAKSVGDNSKKAATKLKLNRNIHDLEKVKETAYTEMGKQYFLSHADAAEEPYAEWVQKIVEADQQIAAFKKEIAALDDLRICSACGAEMAEGQKFCPNCGAKNEVPEKQPEETKTESVSEEEVEILSTEETKTEDTPDQPSES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>R5VI89</accession>
    <name>R5VI89_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Thioredoxin-disulfide reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_01057</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01057"/>
    <dbReference type="EnsemblBacteria" id="CCZ83276">
        <property type="protein sequence ID" value="CCZ83276"/>
        <property type="gene ID" value="BN566_01057"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016668">
        <property type="term" value="F:oxidoreductase activity, acting on a sulfur group of donors, NAD(P) as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008255">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_2_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00573">
        <property type="entry name" value="PYRIDINE_REDOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="4">
        <location>
            <begin position="2"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="301" mass="32063" checksum="41845C37EAD0F984" modified="2013-07-24" version="1">MYDIIIIGSGPAGLTAAIYASRAQLTAVVLEKDYLGSGQIAVSEQVDNYPGLPGIDGYTLGEKFREHAEQLGAKFENVEVDHIKQTAEGFTVFCKEGEPLQSRTILCASGTSYRRLDVPGGTLQGVSYCATCDGAFYKDKTVAVIGGGDTALGDAMYLSRMAKKVYLVHRRQEFRANQALQTAVQQNAAIELVLDAVPTAVLGEKRVDHLELLQNGASKTLPVDGVFVAIGSIPNTDFLGDLCKLDESGYILADETGVTTTPGVFAAGDVRTTPLRQVVTAVADGANCVQSIEQYFRKGNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5VIX1</accession>
    <name>R5VIX1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative small GTP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01235</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000110">
        <property type="protein sequence ID" value="CCZ83461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01235"/>
    <dbReference type="EnsemblBacteria" id="CCZ83461">
        <property type="protein sequence ID" value="CCZ83461"/>
        <property type="gene ID" value="BN566_01235"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03711">
        <property type="entry name" value="Tet_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010298">
        <property type="entry name" value="DUF901"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035650">
        <property type="entry name" value="Tet_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34547">
        <property type="entry name" value="PTHR34547"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05991">
        <property type="entry name" value="NYN_YacP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="SSF54980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EFG_C" evidence="1">
        <location>
            <begin position="7"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="361" mass="41133" checksum="A6099AE7A15559F6" modified="2013-07-24" version="1">MLSTESVLLEPWYQFRLTIPTECVGRAIADLQHMSATFSAPEQATPTTQVLQGTAPVSELRTYSETVVAYTKGKGRLFCQMKGYFPCHNTEEVLEQVSYDGANDPENSGDSIFCSHGAGVLVKWDDVPKHMHLPYAYVPEQKAELTPPVQLARQVERYRSRLEEDQALLGWFERTYGKIQRDPRQALDRLPDDVTEAAKTAPLQPLKTGDVYLLVDGYNVIHAWDSLKQYLPDHLELARKQLSDWLCNYQGFRQCQVILVFDAYQVKGGREHVEQYHNIHIVYTKEAETADAYIERTTYDLAKERRVRVVTSDGLEQRIILGNGACRVSAAEFEKELKEVEQAIQSYLQSGHHPSNHITIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5VIY3</accession>
    <name>R5VIY3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00009</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000112">
        <property type="protein sequence ID" value="CCZ83471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00009"/>
    <dbReference type="EnsemblBacteria" id="CCZ83471">
        <property type="protein sequence ID" value="CCZ83471"/>
        <property type="gene ID" value="BN566_00009"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="334"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="359" mass="40760" checksum="3F2BE3AF55A78A0D" modified="2013-07-24" version="1">MNTVQYKCPNCGGELVFNPEKQMFSCPYCMSDFTEAEMEERYHTQNETAQKEADADAKTLKDENDEAAFEEHTRLYECPSCGAQIIADETTSATFCYYCHNPVVLAGRLKGNYKPAYVLPFAVQRDAAEKEFRAWCRKRWFLPSDFRSKQQLEKMSGVYVPFWLASCVADTDMSGVGQVVKSWRDGKYQVTETTEYTVSRQAKIPFERVPADGAAKIEDALMDAIEPFPVQEMRPFSMQYLSGFLAEKYDVDWEKIVPRIQSRVQTASEQVINETLTQYTSIKNKQVHVNMTNLQHDYVLLPVWFMSYQHGGKTYHFAMNGQTKKLSGTPPLSWAKLLTFCAALMVVIGVLGGLIGGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJ12</accession>
    <name>R5VJ12_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Acetyltransferase GNAT family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01260</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000117">
        <property type="protein sequence ID" value="CCZ83491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01260"/>
    <dbReference type="EnsemblBacteria" id="CCZ83491">
        <property type="protein sequence ID" value="CCZ83491"/>
        <property type="gene ID" value="BN566_01260"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045057">
        <property type="entry name" value="Gcn5-rel_NAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031165">
        <property type="entry name" value="GNAT_YJDJ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31435">
        <property type="entry name" value="PTHR31435"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14542">
        <property type="entry name" value="Acetyltransf_CG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51729">
        <property type="entry name" value="GNAT_YJDJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="4"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="86" mass="9756" checksum="CAF0216B1B404D50" modified="2013-07-24" version="1">MQKSYQKNEHEIYLENEAGERIAEILFPEVEPKVYCIQRTFVDDSLRGQGVAGTLMELAVQAIQEQGGTVTATCSYAQHWLEKHAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJX8</accession>
    <name>R5VJX8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cell shape-determining protein MreB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">mreB</name>
        <name evidence="3" type="ORF">BN566_01581</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Forms membrane-associated dynamic filaments that are essential for cell shape determination. Acts by regulating cell wall synthesis and cell elongation, and thus cell shape. A feedback loop between cell geometry and MreB localization may maintain elongated cell shape by targeting cell wall growth to regions of negative cell wall curvature.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Forms polymers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="2">Membrane-associated.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the FtsA/MreB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000166">
        <property type="protein sequence ID" value="CCZ83846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01581"/>
    <dbReference type="EnsemblBacteria" id="CCZ83846">
        <property type="protein sequence ID" value="CCZ83846"/>
        <property type="gene ID" value="BN566_01581"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd10225">
        <property type="entry name" value="MreB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02207">
        <property type="entry name" value="MreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004753">
        <property type="entry name" value="MreB"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01652">
        <property type="entry name" value="SHAPEPROTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00904">
        <property type="entry name" value="mreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0133">Cell shape</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="12"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="156"/>
            <end position="158"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="204"/>
            <end position="207"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="284"/>
            <end position="287"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="337" mass="36324" checksum="C59FEF2CB0C56F22" modified="2013-07-24" version="1">MFIKDIGIDLGTANTLIYLRGRGIIIREPSVVAVNTHTDSAKYVGKEAKEVIGRTPGSIVAVKPLKNGVIADFETTTIMLQEFIRKALRGKTFTKARVIICVPSGVTTVERRAVKEAVEQAGAKYVLIVEEAMAAAIGAGLPTLEPIGSMIVDIGGGTSEAAVISMGGIISSKSIRCGGDELDAAIINHIRKRYNLLIGERTAEDIKIQIGSAFPMEREDAMEIKGRNLLNGLPETINITGSEVRDAMSEPLARIVDAIKYTLEQTPPDLSADIIDRGIILSGGGALLRGIDRLIHKETGMPVLVAEYPLDCVAEGTGKLFSNLERYQDAFMEDNRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJZ1</accession>
    <name>R5VJZ1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00166</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000168">
        <property type="protein sequence ID" value="CCZ83861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83861">
        <property type="protein sequence ID" value="CCZ83861"/>
        <property type="gene ID" value="BN566_00166"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="251"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="278" mass="32702" checksum="78500F8372F56740" modified="2013-07-24" version="1">MVQPIPEYRGNLRHLIHRSGFCLNHGCQRQGLIQRQIQFVHSFVVFRCNLTVEVLQNLLAHQVYIHIFIENIGVRKQIAFHRIELFIDKARQVGFIRIAVFCILVEKFQEFFCLANARALQHVSCFSQVDALRKCNLYRSGFVHCWRCQFFKDGFIRHIRTKFMFTSGELSIFITSSCKERKHFRMADDASFFQNVGNFTCIRTGRNVYNTSFRIVINAKQVPTKCHGQSGNHNGNRNDCKRNNQCGQHSALPPFFLFLLFLLLLWRYWCWLLLTDAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>R5VKA6</accession>
    <name>R5VKA6_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">L-aspartate oxidase</fullName>
            <ecNumber evidence="4">1.4.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN566_01685</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-aspartate + O2 = H2O2 + iminosuccinate</text>
            <dbReference type="Rhea" id="RHEA:25876"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
            <dbReference type="EC" id="1.4.3.16"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:25877"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (oxidase route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.16" evidence="4"/>
    <dbReference type="EMBL" id="CBAR010000176">
        <property type="protein sequence ID" value="CCZ83956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01685"/>
    <dbReference type="EnsemblBacteria" id="CCZ83956">
        <property type="protein sequence ID" value="CCZ83956"/>
        <property type="gene ID" value="BN566_01685"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00326"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008734">
        <property type="term" value="F:L-aspartate oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044318">
        <property type="term" value="F:L-aspartate:fumarate oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.700.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-binding_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005288">
        <property type="entry name" value="NadB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027477">
        <property type="entry name" value="Succ_DH/fumarate_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42716">
        <property type="entry name" value="PTHR42716"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56425">
        <property type="entry name" value="SSF56425"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD_binding_2" evidence="10">
        <location>
            <begin position="12"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ83956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="417" mass="45286" checksum="4131684E22370E1E" modified="2013-07-24" version="1">MMVTKLRIGDANTMMAEGGIQAADKPNDSPAQHFLDAYGGGHFAADKELVYKLVTEAPDCIQWLNKLGVEFDKDKDGNMITTHGGGTSRKRMHAAKDYSGAEIMRTLRDEVLNRQIPVIDFTSAIELILDDKGHCAGAVLLNMETKEILIAKAKVVIIATGGAGRMHYQGFPTSNHYGATADGLVLAYRVGAPLREQYTLQYHPTGAAFPEQIFGALVTEKVRSLGAKLVNVKGEVFMNPLETRDVTAATVIRECRRGNQVNTPGGQGVWLDTPMIDLKNGEGTIEKRIPAMLRMFGKYGIDIRKQPILIYPTLHYQNGGVKVGTDSQTCVENLFVAGEAAGGVHGENRLMGNSLLDVIVFGRNAGKYAAAKAKDVVVGNLTLDHVAKFEKELEDAGITPDTVSPMLLPDYTRKVND</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>R5VKS8</accession>
    <name>R5VKS8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Endonuclease III</fullName>
            <ecNumber evidence="10">4.2.99.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">DNA-(apurinic or apyrimidinic site) lyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">nth</name>
        <name evidence="12" type="ORF">BN566_01852</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-monophospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="10">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the Nth/MutY family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.99.18" evidence="10"/>
    <dbReference type="EMBL" id="CBAR010000189">
        <property type="protein sequence ID" value="CCZ84126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01852"/>
    <dbReference type="EnsemblBacteria" id="CCZ84126">
        <property type="protein sequence ID" value="CCZ84126"/>
        <property type="gene ID" value="BN566_01852"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019104">
        <property type="term" value="F:DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00942">
        <property type="entry name" value="Nth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004036">
        <property type="entry name" value="Endonuclease-III-like_CS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003651">
        <property type="entry name" value="Endonuclease3_FeS-loop_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004035">
        <property type="entry name" value="Endouclease-III_FeS-bd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000445">
        <property type="entry name" value="HhH_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005759">
        <property type="entry name" value="Nth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10576">
        <property type="entry name" value="EndIII_4Fe-2S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00633">
        <property type="entry name" value="HHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00525">
        <property type="entry name" value="FES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="SSF48150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01083">
        <property type="entry name" value="nth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00764">
        <property type="entry name" value="ENDONUCLEASE_III_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01155">
        <property type="entry name" value="ENDONUCLEASE_III_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4 10" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8 10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="12" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="9 10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 10" id="KW-0408">Iron</keyword>
    <keyword evidence="7 10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="10" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENDO3c" evidence="11">
        <location>
            <begin position="39"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CCZ84126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="209" mass="23615" checksum="5E0A8FE960A747D7" modified="2013-07-24" version="1">MTKQETALRACTILEEYYPESVCALQYKEPYELLIAVRLSAQCTDARVNIVTKTLFQQYPTLESFAAANLEELEQAVKPCGFYHMKAKSIKEMAIRLLTVYDGKIPDTMEELLTLPGVGRKTANLILGDVYKQPAIVTDTHFIRITGRLGLTTNKEPAKVERDLRPLIPPERSSDFCHRIVQFGRDICRARKPLCGNCPMADFCPSREA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>R5VKZ5</accession>
    <name>R5VKZ5_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Queuine tRNA-ribosyltransferase</fullName>
            <ecNumber evidence="4">2.4.2.29</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Guanine insertion enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">tRNA-guanine transglycosylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">tgt</name>
        <name evidence="6" type="ORF">BN566_01931</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>7-aminomethyl-7-carbaguanine + guanosine(34) in tRNA = 7-aminomethyl-7-carbaguanosine(34) in tRNA + guanine</text>
            <dbReference type="Rhea" id="RHEA:24104"/>
            <dbReference type="Rhea" id="RHEA-COMP:10341"/>
            <dbReference type="Rhea" id="RHEA-COMP:10342"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:58703"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:82833"/>
            <dbReference type="EC" id="2.4.2.29"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="4">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">tRNA modification; tRNA-queuosine biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer. Within each dimer, one monomer is responsible for RNA recognition and catalysis, while the other monomer binds to the replacement base PreQ1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the queuine tRNA-ribosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.29" evidence="4"/>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01931"/>
    <dbReference type="EnsemblBacteria" id="CCZ84206">
        <property type="protein sequence ID" value="CCZ84206"/>
        <property type="gene ID" value="BN566_01931"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00392"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008479">
        <property type="term" value="F:queuine tRNA-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008616">
        <property type="term" value="P:queuosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0101030">
        <property type="term" value="P:tRNA-guanine transglycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.105">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00168">
        <property type="entry name" value="Q_tRNA_Tgt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004803">
        <property type="entry name" value="TGT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036511">
        <property type="entry name" value="TGT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002616">
        <property type="entry name" value="tRNA_ribo_trans-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01702">
        <property type="entry name" value="TGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51713">
        <property type="entry name" value="SSF51713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00430">
        <property type="entry name" value="Q_tRNA_tgt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00449">
        <property type="entry name" value="tgt_general"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0671">Queuosine biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 4" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="TGT" evidence="5">
        <location>
            <begin position="11"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="4">
        <location>
            <begin position="89"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="RNA binding" evidence="4">
        <location>
            <begin position="252"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="271"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="311"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="314"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="340"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ84206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="376" mass="42261" checksum="32DD28A0F32D0D9B" modified="2013-07-24" version="1">MYTCIKTEGAARRGVFETVHGTIQTPFFMNVGTAAAIKGGISSVDLANLKCQVELCNTYHLHLRPGDTVIRQMGGLHKFMHWNKPILTDSGGFQVFSLAKLRKIKEEGVYFQSHIDGKRIFMGPEESMQIQSNLASTIAMAFDECVENPSPFDYVKASCARTVRWLERCQKKMAQLNADPNTINQHQLLFGINQGGTFDGLRTAHMKEIATMGLDGYAIGGLAVGEPTDVMYHIIEEVEPFMPKDKPRYLMGVGTPSNIIEAVARGVDMFDCVMPSRNARHGTVFTWDGILHITNACYETDPQPLDPKCDCPVCKNFSRAYIRHLFKAKEQLAGRLAVTHNLYFYNTLLEKIREALDEDRFGAFRSEYSEKLAQRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>R5VLC0</accession>
    <name>R5VLC0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ATPase P-type (Transporting) HAD superfamily subfamily IC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_00259</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000217">
        <property type="protein sequence ID" value="CCZ84346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00259"/>
    <dbReference type="EnsemblBacteria" id="CCZ84346">
        <property type="protein sequence ID" value="CCZ84346"/>
        <property type="gene ID" value="BN566_00259"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="SSF81653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="SSF81665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-1278">Translocase</keyword>
    <keyword evidence="1 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="192"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="230"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="565"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="592"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="635"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="667"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="692"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="733"/>
            <end position="754"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ84346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="758" mass="82410" checksum="2615F832309E03EB" modified="2013-07-24" version="1">MKAKETASKTTAQILRDNVCTLFNLLNLLIAIALIAVHAWSNLFFIFVIALNTVIGIAQELKAKRLIEKLTLLSQPNAKIERNGTIQSVPIAEVQEQDILLLESGNQICADAIVRSGGIEVNESLLTGESDAIFKKAGDTLLSGSSVISGTCQATVLHVGSDSYAAKLTKEAKKTKAMHSELVGSMRKVTRLTSLCIVPLGILLFVQALFFRDSSMYDAVVGTSAGLLGMLPKGLYLLISISLAAGIITLSKKNVLVQDLYSLENLAHVDVLCLDKTGTLTEGNMKVEKAVLFPGTDSAMFQEWMGNFLTHTQDNNATFQAMHTYFDGMDTLSVTEQIPFSSDRKWSAMTFSGAGTLVIGAPERLSPEKLPESVLEAQQNGKRVLLAGITQHAVQKEQPLPEMQLLAEIILTDPLRANAAKTIAAFQQEGVSVKLISGDNPMTASAMAQQAGVFQSDRWVDMRTVADSEMDAAAAQYTVFGRVTPQQKKQLIQAFHRQKHTVAMTGDGVNDLLALKEADCSISVGQGSDAARQTAQLVLLDSDFAVLKDVLLEGRRVVHNVTRSAGVFFIKTLYSILLCVLCLLTNTPFPFVPIQITLIDLVIEGYPSFFLSFLPDSQPVRTKFLPEAIRRAAPNALAIGVCFLFYLVLHAMGIFGLSGEQTQANTLLFLLIGTVGLLGVFKMCLPFTKVKVFFAATSAIGFYFAIALCLWMQQHLLHLDILHLAVPTGTTLLFFGLFAIVAILIASILSRTLFKQKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5VLC6</accession>
    <name>R5VLC6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Csd1 family CRISPR-associated protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00264</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000218">
        <property type="protein sequence ID" value="CCZ84351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00264"/>
    <dbReference type="EnsemblBacteria" id="CCZ84351">
        <property type="protein sequence ID" value="CCZ84351"/>
        <property type="gene ID" value="BN566_00264"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010144">
        <property type="entry name" value="CRISPR-assoc_prot_Csd1-typ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09709">
        <property type="entry name" value="Cas_Csd1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01863">
        <property type="entry name" value="cas_Csd1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="654" mass="75309" checksum="59C3E5C7DEDFF1BC" modified="2013-07-24" version="1">MLRWADELYQIYERFCGQPIGGDTAVLLPVAHSTANAQIEVTLSEDGIFQAAKKIEDKKDAVTIIPVTENSGSRSSGITPHPLADKLIYLAGDYGNYVPEKAEKASKSYAAYLEQLKIWAEENHSHPAVRAIYTYLQKGCLIQDLLAASVLNLDEQTGKLGKEKICEVVQSDCMVRFRVYQLNPGSRDETWLDSTLYESWIEQNEEQNASQQVRQLCYATGKVTECSYKHPNKIRNAGDKAKLFSANDESGFSYRGRFQNKEQAVSIGYDFSQKMHNALRWMIQRQGYSISKTMTVLAWESNLEAIPNLMESSFEMSSGYKEQCKKSQKDDAINIFDIPTNDFGADVASERTFASYREDLIKMIRGYQRNMEPLSNVTVMVLDSATTGRLSMTMYAQMEHSEFFENLKEWHEQTAWRYWRRKEFDIFSFDLRDIVNCAYGTEQKNVLKCDDKLMPFTIGRLLPCVLQKQAFPMDLLQALCDKAAYPQAYEQKYNWDLVVACACGMLRKKICEQKGECNMELDETCTRRDYLYGRLLAIAEVAESVALNKKEGNDTRITNARRYFTAFVNQPYRTWNVIRQRLEPYLSAMPKTQRNYYCTMIDEITSQFDHTAFMDNSKLEPEYLHAYSCQLQKISADKKHKNQNDSNNEEETES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5VLG3</accession>
    <name>R5VLG3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00293</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000222">
        <property type="protein sequence ID" value="CCZ84386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84386">
        <property type="protein sequence ID" value="CCZ84386"/>
        <property type="gene ID" value="BN566_00293"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021529">
        <property type="entry name" value="DUF2798"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11391">
        <property type="entry name" value="DUF2798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="164" mass="18536" checksum="62DC173B24B4C40F" modified="2013-07-24" version="1">MPKNKFQEVIFTILMVFVMVYAMVCYNIALNTGGMSNAVFLAAFRELPIMMPIAFILDFFLVGNLAKKAAFRMVDVKTERPFHLILAISAVSVAWMCPLMSLAATILFQHPGKQIISMWLQTTVFNFPMALCWQLFFAGPLVRFVFGRIFREKATAPAVQPTVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5VLH9</accession>
    <name>R5VLH9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00308</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000224">
        <property type="protein sequence ID" value="CCZ84401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00308"/>
    <dbReference type="EnsemblBacteria" id="CCZ84401">
        <property type="protein sequence ID" value="CCZ84401"/>
        <property type="gene ID" value="BN566_00308"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004395449" evidence="1">
        <location>
            <begin position="30"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="72" mass="7589" checksum="CEDF087FBEB8FCBE" modified="2013-07-24" version="1">MKKQVLAVSAALFLSAVCLGAMRTVPARAAAQETAAVAICGTVIVQQNQVPELPSYTDPVMETTIDEEETPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VM15</accession>
    <name>R5VM15_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00427</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000246">
        <property type="protein sequence ID" value="CCZ84566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84566">
        <property type="protein sequence ID" value="CCZ84566"/>
        <property type="gene ID" value="BN566_00427"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="136" mass="15572" checksum="DD64C4D6D77FEC01" modified="2013-07-24" version="1">MSTPNWIMVTVKTPQTAGDSYMANIMKNHLKEQNCSFHSEEQSAALWLVCRQVSPQRYDFSISDSDSVWTKEAVMELCSTLLQCPEVDNVLVGQVPSHQQDVKVTVSPMVTFLHTVKNYLQPQRISMVLRRLRSNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5VQW5</accession>
    <name>R5VQW5_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Coenzyme A biosynthesis bifunctional protein CoaBC</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">DNA/pantothenate metabolism flavoprotein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Phosphopantothenoylcysteine synthetase/decarboxylase</fullName>
            <shortName evidence="3">PPCS-PPCDC</shortName>
        </alternativeName>
        <domain>
            <recommendedName>
                <fullName evidence="3">Phosphopantothenoylcysteine decarboxylase</fullName>
                <shortName evidence="3">PPC decarboxylase</shortName>
                <shortName evidence="3">PPC-DC</shortName>
                <ecNumber evidence="3">4.1.1.36</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="3">CoaC</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="3">Phosphopantothenate--cysteine ligase</fullName>
                <ecNumber evidence="3">6.3.2.5</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="3">CoaB</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="3">Phosphopantothenoylcysteine synthetase</fullName>
                <shortName evidence="3">PPC synthetase</shortName>
                <shortName evidence="3">PPC-S</shortName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="3" type="primary">coaBC</name>
        <name evidence="7" type="ORF">BN566_00747</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes two sequential steps in the biosynthesis of coenzyme A. In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine. In the second step the latter compound is decarboxylated to form 4'-phosphopantotheine.</text>
    </comment>
    <comment type="function">
        <text evidence="4">Catalyzes two steps in the biosynthesis of coenzyme A. In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>(R)-4'-phosphopantothenate + CTP + L-cysteine = CMP + diphosphate + H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine</text>
            <dbReference type="Rhea" id="RHEA:19397"/>
            <dbReference type="ChEBI" id="CHEBI:10986"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:59458"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="6.3.2.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-phosphopantetheine + CO2</text>
            <dbReference type="Rhea" id="RHEA:16793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:59458"/>
            <dbReference type="ChEBI" id="CHEBI:61723"/>
            <dbReference type="EC" id="4.1.1.36"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="3">Binds 1 FMN per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 4">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 2/5.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 4">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">In the C-terminal section; belongs to the PPC synthetase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">In the N-terminal section; belongs to the HFCD (homo-oligomeric flavin containing Cys decarboxylase) superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.36" evidence="3"/>
    <dbReference type="EC" id="6.3.2.5" evidence="3"/>
    <dbReference type="EMBL" id="CBAR010000030">
        <property type="protein sequence ID" value="CCZ82947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00747"/>
    <dbReference type="EnsemblBacteria" id="CCZ82947">
        <property type="protein sequence ID" value="CCZ82947"/>
        <property type="gene ID" value="BN566_00747"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00241">
        <property type="reaction ID" value="UER00353"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004632">
        <property type="term" value="F:phosphopantothenate--cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004633">
        <property type="term" value="F:phosphopantothenoylcysteine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015941">
        <property type="term" value="P:pantothenate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02225">
        <property type="entry name" value="CoaBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035929">
        <property type="entry name" value="CoaB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005252">
        <property type="entry name" value="CoaBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007085">
        <property type="entry name" value="DNA/pantothenate-metab_flavo_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04127">
        <property type="entry name" value="DFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102645">
        <property type="entry name" value="SSF102645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="SSF52507"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00521">
        <property type="entry name" value="coaBC_dfp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3 4" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="3 4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 4" id="KW-0288">FMN</keyword>
    <keyword evidence="3 4 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 3 4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavoprotein" evidence="5">
        <location>
            <begin position="5"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="DFP" evidence="6">
        <location>
            <begin position="185"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphopantothenoylcysteine decarboxylase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphopantothenate--cysteine ligase" evidence="3">
        <location>
            <begin position="190"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="323"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="337"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="341"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ82947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="397" mass="43060" checksum="A8AC8686F0E2027F" modified="2013-07-24" version="1">MLTGKTVVLAVTGSIAAYKIANLARMLKKLHADVQVLMTQNATNFINPITLETLTNQKCLIDTFDRNFQYSVEHVALAKRADVVLVAPASANVIGKLANGIADDMLTTTILACPCKKWISPAMNHNMYHNPIVQDNIQKLERFGYGIISPATGMLANGDTGDGRMPEETVLLEYLLREIAYPKDLAGKKFLISAGATQEALDPVRFLTNHSSGKMGCALARAAMQRGAEVTLVAAHMEVAPPPFVKVVRVTSAADMAEAILPVAAEYDCIIKAAAVSDYTPVATSTEKIKKADGTLTVSFQRTTDILKTLGEHKPEHLFLCGFSMETENVIENSRKKLQSKHCDMICANSLRKAGAGFGGDTNIITILTAEREIELEQMTKDEAAHRILDTILESGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5VRA0</accession>
    <name>R5VRA0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00865</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000051">
        <property type="protein sequence ID" value="CCZ83077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83077">
        <property type="protein sequence ID" value="CCZ83077"/>
        <property type="gene ID" value="BN566_00865"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="111" mass="12174" checksum="95C40127C1D78740" modified="2013-07-24" version="1">MAEKHSIYDCPYCGKKSFNPLTKALAGNMNTKGRVCKACGRRAVNGKTSAIVRTVLMVVAVIVVFATYFIENSDAKAYLIMAGTIIVAWLLSMLFDAFFCPLTKVVRNDAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5VRD7</accession>
    <name>R5VRD7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulators</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00909</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000060">
        <property type="protein sequence ID" value="CCZ83122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00909"/>
    <dbReference type="EnsemblBacteria" id="CCZ83122">
        <property type="protein sequence ID" value="CCZ83122"/>
        <property type="gene ID" value="BN566_00909"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01047">
        <property type="entry name" value="MarR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00598">
        <property type="entry name" value="HTHMARR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="140" mass="15900" checksum="8801F1B88732FCC8" modified="2013-07-24" version="1">MKETLHYLLMTDHFLFQKTLFGKIKETGLSIGQPKVLDYLKEHDGTTQREIAAACQIEPASLTSILNGMEKANLIERKTLDGNRRSWHIFLTEKGKEQLKCVDAAFTEIEQTALQGFTAAEQQALCAYLLRIHKNFESKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>R5VRM9</accession>
    <name>R5VRM9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative transmembrane permease MsmG</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_00995</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000073">
        <property type="protein sequence ID" value="CCZ83212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00995"/>
    <dbReference type="EnsemblBacteria" id="CCZ83212">
        <property type="protein sequence ID" value="CCZ83212"/>
        <property type="gene ID" value="BN566_00995"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="115"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="149"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="188"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="250"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="78"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ83212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="283" mass="31775" checksum="B345271C2FE1C246" modified="2013-07-24" version="1">MKTKKQRIINGIFVAVMLVLSLFFLFPVIWMIANSFKQDAAITADMNSVRAFVPPAPGKGFFDNYKEILFNTSFLRYMLNTLFYAAILIVAGVIVNGLAGYALAKIKFPFRDRWLAIIMMLMIIPTETIITINFMFVSKMGLLNTVFGYVLPMIVNPFNIFLFRQVFVSLPDDVWEAAQLDHCNPVKYFFTMVLPMSKTVVATVSVFTFLNVWNDYLWPSLVFTSSDLLTAQIGLNSITSNDNTTMGQTLAVITLITIPVIIIYALFSKQIVEGVVSTGSKEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5VS27</accession>
    <name>R5VS27_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01128</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000092">
        <property type="protein sequence ID" value="CCZ83352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83352">
        <property type="protein sequence ID" value="CCZ83352"/>
        <property type="gene ID" value="BN566_01128"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024227">
        <property type="entry name" value="DUF3795"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12675">
        <property type="entry name" value="DUF3795"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="139" mass="15961" checksum="4A28DA9BD5C5D373" modified="2013-07-24" version="1">MQKIIAACGNDCSACPRYVAPPYEKSDEELKYTAELWLEIGYRDRLVSNEEISCIGCRPENWCRYLVVKCCEDKGIKNCSECAEFPCDNIRECFAVTKSFEPKCREVCTEAEYEQIKKAFFEKEENLKALQAANIKDGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>R5VSC5</accession>
    <name>R5VSC5_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">ATP-dependent 6-phosphofructokinase</fullName>
            <shortName evidence="15">ATP-PFK</shortName>
            <shortName evidence="15">Phosphofructokinase</shortName>
            <ecNumber evidence="15">2.7.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Phosphohexokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">pfkA</name>
        <name evidence="17" type="ORF">BN566_01206</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 15">Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 1,6-bisphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="15">Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5 15">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 15">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade 'B1' sub-subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.11" evidence="15"/>
    <dbReference type="EMBL" id="CBAR010000103">
        <property type="protein sequence ID" value="CCZ83432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01206"/>
    <dbReference type="EnsemblBacteria" id="CCZ83432">
        <property type="protein sequence ID" value="CCZ83432"/>
        <property type="gene ID" value="BN566_01206"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00182"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003872">
        <property type="term" value="F:6-phosphofructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00339">
        <property type="entry name" value="Phosphofructokinase_I_B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022953">
        <property type="entry name" value="ATP_PFK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012003">
        <property type="entry name" value="ATP_PFK_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012828">
        <property type="entry name" value="PFKA_ATP_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015912">
        <property type="entry name" value="Phosphofructokinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000023">
        <property type="entry name" value="Phosphofructokinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035966">
        <property type="entry name" value="PKF_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00365">
        <property type="entry name" value="PFK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000532">
        <property type="entry name" value="ATP_PFK_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00476">
        <property type="entry name" value="PHFRCTKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53784">
        <property type="entry name" value="SSF53784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02482">
        <property type="entry name" value="PFKA_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00433">
        <property type="entry name" value="PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 15" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="12 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14 15" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="11 15 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="13 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PFK" evidence="16">
        <location>
            <begin position="5"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="ADP binding; allosteric activator; shared with dimeric partner" evidence="15">
        <location>
            <begin position="22"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="15">
        <location>
            <begin position="126"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="15">
        <location>
            <begin position="170"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="ADP binding; allosteric activator" evidence="15">
        <location>
            <begin position="214"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="15">
        <location>
            <begin position="250"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="73"/>
            <end position="74"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="103"/>
            <end position="106"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CCZ83432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="321" mass="34374" checksum="DCF22B9C4CFB0E5E" modified="2013-07-24" version="1">MIKTIGVLTSGGDAPGMNAAVRAVVRAALKKGIKVYGIRRGYCGLIDGDIFEMNEMSVSNIIHKGGTILYTARSAEFRTEAGVEIAKKTCEKLGLEGLVVIGGDGSFRGAADLSAKGIACVGLPGTIDNDIACTEYTIGYDTAMNTCVEMIDKLQDTCQSHDRCSVVEVMGRGAGFIAVNTGLASGAVEIITKEVPYDLDELARKMLECKKNGKQNFIVVVAENIGNAEGIARAIQEKTGIESRATVLGHVQRGGSPTVRDRVVASEMGYYAVELLEKGASNRVVGMQNNKIVDFDIQEALSMKKPYEEHLHQIAQDIAFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>R5VSW9</accession>
    <name>R5VSW9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN566_00159</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CBAR010000146">
        <property type="protein sequence ID" value="CCZ83632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00159"/>
    <dbReference type="EnsemblBacteria" id="CCZ83632">
        <property type="protein sequence ID" value="CCZ83632"/>
        <property type="gene ID" value="BN566_00159"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="173"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="10">
        <location>
            <begin position="192"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="9">
        <location>
            <begin position="252"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="472"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ83632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="494" mass="56383" checksum="693A84C09B896B2A" modified="2013-07-24" version="1">MERNLTIRVKIWLFFMMFVCIVFILMWILQVVFLERFYEAFKIADIAKSAKILTQSCRLDNFSDIAEDQAMRNEMCISILNTDGEETYQHCVSAQKCLLHNDQDVYFFLMDLKRSDNGYICRSIYNEQLQTQTVVYGNWILDSETNETVGYLLLNSRLVPVQSTVSILQRQTMIITIAMAFFGCVLSFLAATHIGVPITRLTRSAKRLAHGDYETKFEGGGCAEVDELAEALTYAEHELSRTDEMQRDLIANVSHDMRTPLTMMKAYAEMIRDLSGNNPEKRAKHLQVIIEETDRLSLLVNDMLDLSKLESGTKKLEFTTFDISQRLTEIANRYQGISEQMGYHLHFTPDASRMVCCDAGAIERVICNLINNAINYTSKEDKQVFLRQVNQPNCVRIEVRDTGDGIEEDKIKLIFDKYYRSENHKREVVGTGLGLSIVKAILKLHNYDYGVNSKLGEGSTFWFAIRTDQQPAPEAPPAIAPTESKKGVDHAKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>R5VT56</accession>
    <name>R5VT56_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phosphopantetheine adenylyltransferase</fullName>
            <ecNumber evidence="6">2.7.7.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Dephospho-CoA pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Pantetheine-phosphate adenylyltransferase</fullName>
            <shortName evidence="6">PPAT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">coaD</name>
        <name evidence="8" type="ORF">BN566_01453</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Reversibly transfers an adenylyl group from ATP to 4'-phosphopantetheine, yielding dephospho-CoA (dPCoA) and pyrophosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>(R)-4'-phosphopantetheine + ATP + H(+) = 3'-dephospho-CoA + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19801"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57328"/>
            <dbReference type="ChEBI" id="CHEBI:61723"/>
            <dbReference type="EC" id="2.7.7.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 4/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the bacterial CoaD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.3" evidence="6"/>
    <dbReference type="EMBL" id="CBAR010000155">
        <property type="protein sequence ID" value="CCZ83717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01453"/>
    <dbReference type="EnsemblBacteria" id="CCZ83717">
        <property type="protein sequence ID" value="CCZ83717"/>
        <property type="gene ID" value="BN566_01453"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00241">
        <property type="reaction ID" value="UER00355"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004595">
        <property type="term" value="F:pantetheine-phosphate adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02163">
        <property type="entry name" value="PPAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00151">
        <property type="entry name" value="PPAT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001980">
        <property type="entry name" value="PPAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21342:SF1">
        <property type="entry name" value="PTHR21342:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01020">
        <property type="entry name" value="LPSBIOSNTHSS"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01510">
        <property type="entry name" value="coaD_prev_kdtB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 6 8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CTP_transf_like" evidence="7">
        <location>
            <begin position="6"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="10"/>
            <end position="11"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="90"/>
            <end position="92"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="125"/>
            <end position="131"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="6">
        <location>
            <position position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="165" mass="18617" checksum="F1915B0E816BC2C2" modified="2013-07-24" version="1">MKRIAVYPGSFDPVTVGHLDVIRRASKLFDELIVLVSKNIMKSNESFTPQERVSMITRVTTDLPNVRVDLSGGLLVDYVRDVHAIAIVKGLRAVSDFEYEFQMALANKKLYDQAETIFLTACTENMYLSSSVVKQIAAFNGDIRHFVPDVLHDEILQRLSKELPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5VT60</accession>
    <name>R5VT60_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Collagenase and related proteases</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00165</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000157">
        <property type="protein sequence ID" value="CCZ83722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00165"/>
    <dbReference type="EnsemblBacteria" id="CCZ83722">
        <property type="protein sequence ID" value="CCZ83722"/>
        <property type="gene ID" value="BN566_00165"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001539">
        <property type="entry name" value="Peptidase_U32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032525">
        <property type="entry name" value="Peptidase_U32_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01136">
        <property type="entry name" value="Peptidase_U32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16325">
        <property type="entry name" value="Peptidase_U32_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01276">
        <property type="entry name" value="PEPTIDASE_U32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_U32_C" evidence="1">
        <location>
            <begin position="323"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="408" mass="45520" checksum="63648533F41AD5A1" modified="2013-07-24" version="1">MNIRKPEVLAPAGDWERFQFALQYGADAIYLGGKQFTMRAAPANFTAEQLAAAVQLAHAKGVKVYLTCNTLPHNADLPQFLPYLREMAETGIDAVIVADLGLLALIRKALPDLEIHMSTQMGIVNYETANALYDLGVKRVVLARELSLEEIAEIRAKTPADLELEAFVHGAMCVSFSGRCLLSAYLTGRDANQGACAQPCRWKYRLMEETRPGQYFPVEEHDEGTYIMNAKDLCMIDHIPELLAAGVDSLKLEGRAKSSYYVAVVTNAYRSAVDAYLQQPEGFVLPEWIREEVYKVSHRGYCTGFFFGKPESGQRYTDNSYDRSFDVIAIADDWRDGMLYVTQRNRFFAEDTVELLAPEQQPISIPLNGNLFDETGERITVANHAMMACAFPCPVAVSKGTIVRKRAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5VT79</accession>
    <name>R5VT79_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Stage II sporulation protein R</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01482</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000161">
        <property type="protein sequence ID" value="CCZ83747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01482"/>
    <dbReference type="EnsemblBacteria" id="CCZ83747">
        <property type="protein sequence ID" value="CCZ83747"/>
        <property type="gene ID" value="BN566_01482"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014202">
        <property type="entry name" value="Spore_II_R"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09551">
        <property type="entry name" value="Spore_II_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="224" mass="25696" checksum="9C6A5F2EB990B567" modified="2013-07-24" version="1">MKRTIKLWEIALLIGCLLTLLFGNLFQAEKTVSDLQQHVLRMHILANSDSDADQQLKYQVRDALLASSETLFGSSQPESREALETTAKAHLHEMETIAQQVVQEQGYSYPVKAELVTMPFTERTYEDLTMPAGTYEAIRITIGAAAGKNWWCVMYPPLCIPVAEDVAVQDEQAKAYFSDAEQDVLYHPERYRVKWKLAEWFKKWTTSEQTDHTTSHISDLRTFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5VTJ9</accession>
    <name>R5VTJ9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NTP pyrophosphohydrolases including oxidative damage repair enzymes</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01587</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000167">
        <property type="protein sequence ID" value="CCZ83852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01587"/>
    <dbReference type="EnsemblBacteria" id="CCZ83852">
        <property type="protein sequence ID" value="CCZ83852"/>
        <property type="gene ID" value="BN566_01587"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="1">
        <location>
            <begin position="39"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="183" mass="20772" checksum="75ABA608C403AEF2" modified="2013-07-24" version="1">MHLNEITKSSDLVYQAKIFHVTKDTAELEDGKIVQRDVVHHSGGVCVVPLTERQTVLMVQQYRYPFHEVTLEIPAGKREPGEDPLECGLRELREEVGRTCKKYTELGYLYPTPAYDTEVIHMYLAEDLSAPMQQDLDAGEFLDVLELPLEEVVTMIMDGKIADAKTQLAILKTWALLQIQGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>R5VU39</accession>
    <name>R5VU39_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Immunoglobulin A1 protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_01743</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000180">
        <property type="protein sequence ID" value="CCZ84017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01743"/>
    <dbReference type="EnsemblBacteria" id="CCZ84017">
        <property type="protein sequence ID" value="CCZ84017"/>
        <property type="gene ID" value="BN566_01743"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008969">
        <property type="entry name" value="CarboxyPept-like_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032774">
        <property type="entry name" value="WG_beta_rep"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14903">
        <property type="entry name" value="WG_beta_rep"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49464">
        <property type="entry name" value="SSF49464"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="SSF52743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 5" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Peptidase_S8" evidence="4">
        <location>
            <begin position="201"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ84017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="1363" mass="152276" checksum="450D026913B81F18" modified="2013-07-24" version="1">MRDAKNSEMKKMLAGTIALLVVVASVGVYQKWFSKTSKFDEDHIGECYYQEIQEEHVATDDLTMYTDNEILLVTEDGVKKRAVEKLAKKYDAEIVGRIEQTGDYQLQLSNVYSLEELEQLSEDLKAEDIVASADINYIFDAEPSDVESSSESFQYGKEWAGDLSNSTDCAGKSWGFEMIQAQAAWELLKEHSDQVNQDLRIGLIDGGFDDNHKDLGFAETFYNQNISEDDKKHGTHVAGIMAAKSDNEAGICGVYPFADGHLYGVSYRGIDNTKNLRSVISDKYSLSELILRNVKVVNCSFNYEDYGVLATAYNEIGAQECMTHYAKTIGDFLQKLLDIGYDFCIIQSAGNSSNENYTRIKYEKNDDISYDQNSKILAKIEELGIGCNIINPKTDKAVFKLLWDKGTIIHAKDLDTKYMSYLTAIEKSDFPDVYDRIIVVGAVDKNRERCDYSNGAASGAGNRGVDIYAPGGNGDSTDNKIFSLQPKNVYDYMCGTSMAAPHVSGVAAMVWSANSSLTGAEVKDIVCNTNRDDSWDLNIVNAESAVGRALNWESDGEETATEPQNGGIFNFVVDKNNNNTKIDHADVTAVKVNGESYTTTADEDGHFELFLPEVTYDITVSRWDYCPYTWKDVEIRNNSVNDLPDWTRLEKRAEWKVEPTIEAEDILVSDEYGNNIAYDQCSIIKQNGKYGIIQYDGSYIANPEYEDYFYDSVYEIAVSDPRVENSDTIKDTLVTPDDGELNISTDTAWGRGNITNKYIYDETSNQIYCITAPPDRANVYSKSIGTLVEDYRTDILGEYGEDNNGYAVSRDITFFNETSPKYGIAKDGKLIVPCEYEDGCMNFSGGVTALKKDGKWGYFDQNGTQIIDFICEPFASKVLYGGWDKGKGTSVNYPFLASDYIPVKIDGKCGYYDTSGNEVIPCGTFEDVRPVHNHLAWVKKDGKWGVIELKELENQTITAVEKSESELHDLMEEKGTICAWECADYDGDGKKEAFAIIGTNNMPDSYISDNIQGVYYINSVGEITELQRNNNPMCRIARSVFHKGKGFFAYDSTAGGSSSQLYLYGVKDGNCFELSISGTITDFYQKDGTCFAVAKTALSSGGYDWLEYELSYDESTQEFTLPKEAVGQATQQTEETTEQSVSSNIPDSYFLAVVNRYLREHDGELDSWLDGLEPYCSSEYSASNETRWSCPINTNRQVYSREQIAGAYPLFAYVDKTTMKGVISVNYKTVAEFDIPAYQSNSTDISTDTNSYIDTYYGSIEKDGHVYSYKMSYHTAYDGSGYFKVIGAVSDGIGGEFELPVTKIEDDVYYYENGSCFNRGANGSRINAASGLSGTVTIQNYGTWIWKIDDFFPYELVLELLQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5VU65</accession>
    <name>R5VU65_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">RelA/SpoT domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01778</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; ppGpp biosynthesis; ppGpp from GTP: step 1/2.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000182">
        <property type="protein sequence ID" value="CCZ84052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01778"/>
    <dbReference type="EnsemblBacteria" id="CCZ84052">
        <property type="protein sequence ID" value="CCZ84052"/>
        <property type="gene ID" value="BN566_01778"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00908">
        <property type="reaction ID" value="UER00884"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015970">
        <property type="term" value="P:guanosine tetraphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd05399">
        <property type="entry name" value="NT_Rel-Spo_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007685">
        <property type="entry name" value="RelA_SpoT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04607">
        <property type="entry name" value="RelA_SpoT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00954">
        <property type="entry name" value="RelA_SpoT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RelA_SpoT" evidence="2">
        <location>
            <begin position="69"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="226" mass="25957" checksum="FC06F3D1C11FD6AD" modified="2013-07-24" version="1">MDDKEFYLDAIKGIVPEQKEVQLQNVFYSFLEIEHLYDSAINVVKTQLNILNNEFNMRFQRNPIHSIESRIKTPQSIIGKLQRKGFAVSPESAREHLQDIAGVRVICCYIDDIYALADLLTVHKDFHLVKTKDYIKNPKPNGYRSFHMIIEVPIYTSTGKQIAPVEIQIRTIAMDFWASLEHQLHYKSIGNATASSSLTGELKHCAETIASIDLKMQEMFNQINNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VUP0</accession>
    <name>R5VUP0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00176</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000195">
        <property type="protein sequence ID" value="CCZ84237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84237">
        <property type="protein sequence ID" value="CCZ84237"/>
        <property type="gene ID" value="BN566_00176"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="323" mass="36254" checksum="128567FCDF491527" modified="2013-07-24" version="1">MLVDDNGCSYHDITNCKTDITFCSIVIEVAICCICVNRVCCSCSIGNCSIGQRKGCRTFCDFIRLSIGFRSKFTSGFCQGVIAVRQSLDNCISTGRNPLNTNLLLIFIQNSGAVFQCNLAPIDIIFRGQGQGCTCKFRLLCDVIFADDNRRFLRVVSNQLRSPNVCINENFIIVNVNIFSRVIRQNDLTVCIDSKEDWVHNCIIFRSGGFYQNIFRIFCEIGSDFVCNIFLNCRQSRFLSVLICFICKFGINCKLCTCQCCTVLVCKVFLDQCPCNTYSLIGNRNCTSRSSIKPTIQQHCTINSSTVRTIYIIINGIIAVCTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>R5VV32</accession>
    <name>R5VV32_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Band 7 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_00284</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the band 7/mec-2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000222">
        <property type="protein sequence ID" value="CCZ84377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00284"/>
    <dbReference type="EnsemblBacteria" id="CCZ84377">
        <property type="protein sequence ID" value="CCZ84377"/>
        <property type="gene ID" value="BN566_00284"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018080">
        <property type="entry name" value="Band_7/stomatin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001972">
        <property type="entry name" value="Stomatin_HflK_HflKC_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00721">
        <property type="entry name" value="STOMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="SSF117892"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01270">
        <property type="entry name" value="BAND_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PHB" evidence="4">
        <location>
            <begin position="19"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="318"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="169"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ84377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="342" mass="37912" checksum="C48D8F738B0C9B60" modified="2013-07-24" version="1">MPLIVLFLLVVFILIVLFTRIKIVPQSNIYVVERLGKFYAAWETGVHFLCPFLDRVAKRVSIKEQVVDFKPQPVITKDNVTMQIDTVVFFQITNPVQFAYGVERPLAAIENLTATTLRNIIGELEFDATLNSRDLINTKITETIDHATDRWGIKVIRVELKNIDPPVDIKNAMEKQMKAEREKREKILRAEGEKEYQIKVAQGEQEAKVLRAEADKKAAILHAEAEKEATILRGEAIKEQKIRIADGEARSIEVVQQALADSLVQLNKANPNAAVLQLKALEAFQKAADGKATKIIIPSEIQGLAGLAAGLKGIVESDQPTESSETVAQVAKNGTTISQDAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVI7</accession>
    <name>R5VVI7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00396</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000240">
        <property type="protein sequence ID" value="CCZ84497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84497">
        <property type="protein sequence ID" value="CCZ84497"/>
        <property type="gene ID" value="BN566_00396"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="181" mass="20836" checksum="6C89D5BE61E00456" modified="2013-07-24" version="1">MKQNNLYGNQIAIELPVKVGKENNAYIQSFNCGNEVMNEYLKYQAEDDAQAVTFILIDQTARAIIGYYSLSCSGFIVESMGHFTIYPAVEIKMFAIDERYQHLPYSAEKEDGTFSDYIFRFVISKIYEFTEEICGADKVILYAVPKAKNFYEKNGFTVFPPFMLQSESRFLEGCIPMYLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVJ9</accession>
    <name>R5VVJ9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00053</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000241">
        <property type="protein sequence ID" value="CCZ84507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84507">
        <property type="protein sequence ID" value="CCZ84507"/>
        <property type="gene ID" value="BN566_00053"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025373">
        <property type="entry name" value="DUF4363"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14276">
        <property type="entry name" value="DUF4363"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004407407" evidence="2">
        <location>
            <begin position="24"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="84"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="120" mass="13420" checksum="F4925AE704575D5A" modified="2013-07-24" version="1">MTRFRISLAILLILLGISIGSMAAVRYSGNAVLEAVQQVSETASPTAAQCEAVETAWEKAEPWLLLSVRRDKLMDTSNSVYRLKPLLESDCDEFTAELKDLQSNLQFLIQEDGFSFLMLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVL8</accession>
    <name>R5VVL8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 8">30S ribosomal protein S13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rpsM</name>
        <name evidence="11" type="ORF">BN566_00083</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA. In the 70S ribosome it contacts the 23S rRNA (bridge B1a) and protein L5 of the 50S subunit (bridge B1b), connecting the 2 subunits; these bridges are implicated in subunit movement. Contacts the tRNAs in the A and P-sites.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Part of the 30S ribosomal subunit. Forms a loose heterodimer with protein S19. Forms two bridges to the 50S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 9">Belongs to the universal ribosomal protein uS13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000241">
        <property type="protein sequence ID" value="CCZ84537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00083"/>
    <dbReference type="EnsemblBacteria" id="CCZ84537">
        <property type="protein sequence ID" value="CCZ84537"/>
        <property type="gene ID" value="BN566_00083"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.910.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01315">
        <property type="entry name" value="Ribosomal_S13_S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027437">
        <property type="entry name" value="30s_Rbsml_prot_S13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001892">
        <property type="entry name" value="Ribosomal_S13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010979">
        <property type="entry name" value="Ribosomal_S13-like_H2TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019980">
        <property type="entry name" value="Ribosomal_S13_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018269">
        <property type="entry name" value="Ribosomal_S13_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10871:SF1">
        <property type="entry name" value="PTHR10871:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00416">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002134">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46946">
        <property type="entry name" value="SSF46946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03631">
        <property type="entry name" value="uS13_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00646">
        <property type="entry name" value="RIBOSOMAL_S13_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50159">
        <property type="entry name" value="RIBOSOMAL_S13_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 8 9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 8" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="2 8" id="KW-0820">tRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="98"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ84537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="122" mass="13746" checksum="8BA1F621735AA2BC" modified="2013-07-24" version="1">MARIAGIDLPKNKRVQIGLTYIYGIGRSTADEILARTGVNPDVRVKDLSEEDVAKLRDDIDKHCIVEGDLRRNVAMDIKRLVEIGCYRGVRHRKGLPVRGQRTKTNARTRKGPVKTIANKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVW3</accession>
    <name>R5VVW3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00520</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000267">
        <property type="protein sequence ID" value="CCZ84667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00520"/>
    <dbReference type="EnsemblBacteria" id="CCZ84667">
        <property type="protein sequence ID" value="CCZ84667"/>
        <property type="gene ID" value="BN566_00520"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002105">
        <property type="entry name" value="Dockerin_1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003646">
        <property type="entry name" value="SH3-like_bac-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00404">
        <property type="entry name" value="Dockerin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08239">
        <property type="entry name" value="SH3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00287">
        <property type="entry name" value="SH3b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51781">
        <property type="entry name" value="SH3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004407424" evidence="1">
        <location>
            <begin position="32"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="SH3b" evidence="3">
        <location>
            <begin position="262"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="2">
        <location>
            <begin position="411"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="480" mass="50541" checksum="67EDEEF537E1E610" modified="2013-07-24" version="1">MFKKLSAVACSCITVLLLLLSLIGGAVHTAAATLDEMTSTAISCIMSHEGSYGSVNRNDCGAVSIGKLQWHAGRALGLLQSICYSAPDLSQSTLGSTLYNEILSSSNWNYRTFTVSEGSAVATLLTTDAGIAAQDALAYSDVQGYIVSGQNKGLVSEGALVMYADIYNFGSGIASRIASRAAGYAGSYGAVSLDDMYRAAMNDSYNSDAAFVQRTNMVYQSLLSMNIGDGDTETTAPVTDPTDEPTQETTTVVTSDFSDSYAGTYVVTASALNLRSNPSTSASVVATLPKNAVVTVTMADGKWAAVTYEGKNGYCSMDYLTVMPDVTTVPEEETTTTTTETTTTTEEMTTISEEITTTEETTTTTEAITMEETTTVETDAEVTETETSATEETVTTTTAAAIDDNKNHGVAATLYGDVDGDGEVTAIDAVLLQKHINGMVFLSHEQMANADCVADGVLDEADVAVILQHLIGHYTALPIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5UWP6</accession>
    <name>R5UWP6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_00003</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000001">
        <property type="protein sequence ID" value="CCZ82748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00003"/>
    <dbReference type="EnsemblBacteria" id="CCZ82748">
        <property type="protein sequence ID" value="CCZ82748"/>
        <property type="gene ID" value="BN566_00003"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010540">
        <property type="entry name" value="CmpB_TMEM229"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06541">
        <property type="entry name" value="ABC_trans_CmpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="147"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ82748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="250" mass="29458" checksum="3EDA7F0880CA3932" modified="2013-07-24" version="1">MNTLLTLAFLFAVGSFLGWCLEVVFRHFVTHKWINPGFLIGPYLPLYGFSLCMLYALARLEPYIPIQNYILKKLVLFVIMAICITAIEYIAGLIFIKGMNIKLWDYSDQWGNVQGIICPLFSFFWLLLSATYYFFIHPYILNSLDWLAQNLAFSFIIGFFFGIFVLDLVYSLRLVRRIRLFAAEHQIVVRLEELKANILASKERNGEKRRFLFAFRSGIPLKEHLERYLDTPSVKQRLKDLRERMQKKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5UX92</accession>
    <name>R5UX92_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Acetolactate synthase small subunit</fullName>
            <shortName evidence="8">AHAS</shortName>
            <shortName evidence="8">ALS</shortName>
            <ecNumber evidence="8">2.2.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Acetohydroxy-acid synthase small subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN566_00723</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the conversion of 2 pyruvate molecules into acetolactate in the first common step of the biosynthetic pathway of the branched-amino acids such as leucine, isoleucine, and valine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>H(+) + 2 pyruvate = (2S)-2-acetolactate + CO2</text>
            <dbReference type="Rhea" id="RHEA:25249"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="2.2.1.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 8">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 8">Dimer of large and small chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 8">Belongs to the acetolactate synthase small subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.6" evidence="8"/>
    <dbReference type="EMBL" id="CBAR010000029">
        <property type="protein sequence ID" value="CCZ82923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00723"/>
    <dbReference type="EnsemblBacteria" id="CCZ82923">
        <property type="protein sequence ID" value="CCZ82923"/>
        <property type="gene ID" value="BN566_00723"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00055"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00059"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990610">
        <property type="term" value="F:acetolactate synthase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04878">
        <property type="entry name" value="ACT_AHAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004789">
        <property type="entry name" value="Acetalactate_synth_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027271">
        <property type="entry name" value="Acetolactate_synth/TF_NikR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019455">
        <property type="entry name" value="Acetolactate_synth_ssu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039557">
        <property type="entry name" value="AHAS_ACT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30239">
        <property type="entry name" value="PTHR30239"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10369">
        <property type="entry name" value="ALS_ss_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00119">
        <property type="entry name" value="acolac_sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ACT" evidence="9">
        <location>
            <begin position="5"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU368092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ82923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="165" mass="18623" checksum="463A21D09B90AF37" modified="2013-07-24" version="1">MERFIISILVANHSGVLSRVSGMFTRRGFNIDTLTVGETESPEFSRITISMHDDEAICNQIVKQLEKMYDVKKVQVMQRDETVARELLLIKIKNNAAERQDIMSVVDVFRAKITDYSPEALVIEIRGVSTKINAFIELLEPYGILEMCRTGLVALERGGNCLKNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXM5</accession>
    <name>R5UXM5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Phosphoribosylformylglycinamidine synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_00630</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000011">
        <property type="protein sequence ID" value="CCZ82825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00630"/>
    <dbReference type="EnsemblBacteria" id="CCZ82825">
        <property type="protein sequence ID" value="CCZ82825"/>
        <property type="gene ID" value="BN566_00630"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010141">
        <property type="entry name" value="FGAM_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041609">
        <property type="entry name" value="PurL_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18072">
        <property type="entry name" value="FGAR-AT_linker"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="SSF55326"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="SSF56042"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01857">
        <property type="entry name" value="FGAM-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FGAR-AT_linker" evidence="5">
        <location>
            <begin position="183"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="AIRS_C" evidence="4">
        <location>
            <begin position="435"/>
            <end position="585"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF18072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ82825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="1234" mass="135722" checksum="BA9B72B7A2C2AB92" modified="2013-07-24" version="1">MSVYRIYVEKKPEFAVEAQSVLNDLQTALRLSVTGVRILNRYDADHLTEEAFQKAIPTVFSEPAVDVTYDTLPECTGNQRMFAAEYLPGQFDQRADSCEQCIQILLQGERCRVRTAQIYIISGNMTDAEFQQIQSYLINPVERRKASLDKPETLDSNYEIPTEVKTLHGFCDRTKEQLDAFIREYGLAMDLDDIQFCQDYFKNTEHRDPTITEIRMIDTYWSDHCRHTTFSTNIEQVEIGAPYIQKTYDAYLNIRHELGRDAKPITLMDMATLAVKKLKADGKLNDLDESEEINACSVKIKVDVAGREEDWILMFKNETHNHPTEIEPFGGAATCLGGAIRDPLSGRSYVYQAMRVTGAANPLVPVEDTIAGKLPQRKITIGAASGYSSYGNQIGLATGHVSEVYHPGYMAKRMEIGAVLGAAPASNIRREAPIPGDVVILLGGKTGRDGCGGATGSSKSHTIESLESCGAEVQKGNAPEERKLQRLFRDPYVTQMIKRCNDFGAGGVSVAIGELTDGLEIDLNAVPKKYDGLDGTEIAISESQERMAVVVAKEDAEKFLQAAGKENLEATIVAEVKAEPRLKMHWNGKNIVNLSREFLNSNGAAKYTDVVIPAPKTDAPAAMSDCAESWKELLSNLNVCSQKGLVEMFDSTIGAGTVLMPFGGAYQLTPSQAMAAKIPVLRGETNTCSLMGWGYNPLISERSPYHGAMCAVVESVAKIVAAGGSWHHCWLTFQEYFERTQNNPERWGKPMAALLGAFQAQLELSCGAIGGKDSMSGTFEQIDVPPTLVSFAVSTAKADHVVSTEFKNTDSKVILVKPTYLENGLPDFDSIRACFDQVEAIIRDNRAAAIWAVDFGGVAEGIAKMCFGNFIGFRFTKQLTPEELFQPCCGAFVIELNGDAKADETVIGTTTNEQTIVCPNYTMQLPQLLQIWEKKLEPVFPCRIKTTTEKPPVYSYNNPVHQFSTIKTARPNVLIPVFPGTNCEYDTARIFERAGARAEVFVIRNLSAAAITESVEAFTDRIKNAQIIMIPGGFSGGDEPEGSGKFITAFFRNPKIMDAVHNLLQNRDGLMLGICNGFQALIKLGLVPFGKIVEMEQDAPTLTFNTIARHQSMLVTTRVASTKSPWLYGCEVGDLHTVAISHGEGRFVAPPALIEQLAENGQIATQYVDLDGNPTMDIHYNPNTSMMAIEGITSPDGRVFGKMGHSERIGTNLYRNVPGEKDQKIFQSGVAYFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXR1</accession>
    <name>R5UXR1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00886</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000055">
        <property type="protein sequence ID" value="CCZ83098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83098">
        <property type="protein sequence ID" value="CCZ83098"/>
        <property type="gene ID" value="BN566_00886"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="122" mass="14542" checksum="61817027673C55BD" modified="2013-07-24" version="1">MKQDSHIYLLLQYYSLFAIPVRLYENKQLIFQKIPFIDTEDPVQLVLPKIMNDNREIGYCLDSNHFCYGFITSSNRKIIVGPVSELEKTKQELRYIAFKLNYQNTEFLIAQMQAICWFFQKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXT5</accession>
    <name>R5UXT5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00920</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000062">
        <property type="protein sequence ID" value="CCZ83133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83133">
        <property type="protein sequence ID" value="CCZ83133"/>
        <property type="gene ID" value="BN566_00920"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="57" mass="6025" checksum="00BAC675380860EF" modified="2013-07-24" version="1">MNLKSVVRGVGVGTAVGTACYLLSKATSRQKHDIRKNANKAMKAVGCVLDDIGSLMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5UY09</accession>
    <name>R5UY09_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00750</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000032">
        <property type="protein sequence ID" value="CCZ82950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82950">
        <property type="protein sequence ID" value="CCZ82950"/>
        <property type="gene ID" value="BN566_00750"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ82950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="67" mass="7347" checksum="1BE4389152BA8918" modified="2013-07-24" version="1">MNETTTAAVAVSESVSEYHIGHTTYRVTTTFNPAFRQSLSDILARLITADCEKMLGETSQEPVQQAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYB6</accession>
    <name>R5UYB6_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glucose-1-phosphate adenylyltransferase</fullName>
            <ecNumber evidence="9">2.7.7.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">ADP-glucose pyrophosphorylase</fullName>
            <shortName evidence="9">ADPGlc PPase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">ADP-glucose synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">glgC</name>
        <name evidence="11" type="ORF">BN566_01099</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in the biosynthesis of ADP-glucose, a building block required for the elongation reactions to produce glycogen. Catalyzes the reaction between ATP and alpha-D-glucose 1-phosphate (G1P) to produce pyrophosphate and ADP-Glc.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>alpha-D-glucose 1-phosphate + ATP + H(+) = ADP-alpha-D-glucose + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:12120"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57498"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="EC" id="2.7.7.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="9">Glycan biosynthesis; glycogen biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the bacterial/plant glucose-1-phosphate adenylyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.27" evidence="9"/>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01099"/>
    <dbReference type="EnsemblBacteria" id="CCZ83318">
        <property type="protein sequence ID" value="CCZ83318"/>
        <property type="gene ID" value="BN566_01099"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00164"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008878">
        <property type="term" value="F:glucose-1-phosphate adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00624">
        <property type="entry name" value="GlgC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011831">
        <property type="entry name" value="ADP-Glc_PPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005836">
        <property type="entry name" value="ADP_Glu_pyroP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023049">
        <property type="entry name" value="GlgC_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43523">
        <property type="entry name" value="PTHR43523"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02091">
        <property type="entry name" value="glgC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00809">
        <property type="entry name" value="ADP_GLC_PYROPHOSPH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00810">
        <property type="entry name" value="ADP_GLC_PYROPHOSPH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7 9" id="KW-0320">Glycogen biosynthesis</keyword>
    <keyword evidence="2 9" id="KW-0321">Glycogen metabolism</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 9 11" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NTP_transferase" evidence="10">
        <location>
            <begin position="9"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Alpha-D-glucose 1-phosphate binding" evidence="9">
        <location>
            <begin position="181"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>alpha-D-glucose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>alpha-D-glucose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>alpha-D-glucose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
        </ligand>
    </feature>
    <feature type="site" description="Could play a key role in the communication between the regulatory and the substrate sites" evidence="9">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <feature type="site" description="Could play a key role in the communication between the regulatory and the substrate sites" evidence="9">
        <location>
            <position position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ83318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="400" mass="44402" checksum="61F3BA309BBB420C" modified="2013-07-24" version="1">MKIKKKCVAMLLAGGQGSRLYALTQKVAKPAIPYGGKYRIIDFPLSNCTNSGVDTVGVLTQYQPLVLNEYIGNGQPWDLDKMHGGVHVLPPYQTAAGASWYEGTANAIYQNMAFIERYDPEYVIVLGGDHIYKMDYSKMLEFHVENDAASTIAVIDVPKEEASRFGIMTCDENRRVTEFTEKPKEPKSTLASMGIYIFTWSKLKAYLIENENANTGSKDFGKDIIPSMLKNEERLFAYEFEGYWKDVGTLDSLWEANMDLLSPSVPLDLYDPQWKIYSRHNNMPPQYVGENAHIENSMITEGSNVDGTVDFSVISAGVTIEAGASVKYSIIMPGTTIKKGAVVEYAIIGENCVIESGARIGKSPESVENRDDWGIAVLGHDITVSENKEVEPKQIISENI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYF2</accession>
    <name>R5UYF2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00843</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000042">
        <property type="protein sequence ID" value="CCZ83050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00843"/>
    <dbReference type="EnsemblBacteria" id="CCZ83050">
        <property type="protein sequence ID" value="CCZ83050"/>
        <property type="gene ID" value="BN566_00843"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018580">
        <property type="entry name" value="Uncharacterised_YfhO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38454">
        <property type="entry name" value="PTHR38454"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09586">
        <property type="entry name" value="YfhO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="129"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="213"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="261"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="342"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="368"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="404"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="430"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="456"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="487"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="920"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="957"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="980"/>
            <end position="1042"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="1042" mass="117543" checksum="B98BE9426487DD0F" modified="2013-07-24" version="1">MTNASTRKSAAAAKRSPLTLAGVGHGLQGWLIRNKFYIIAFFIPVALTYLAYALFGISPFGEESVLCLDLNGQYVYYFESLRDAFWGDGSIFYNWSRNLSGNFMGIIGYYLASPFTLIVMLLPEKWILASLLIMQLCKLGAAGVTFSYYLQKRREVSAPHALLFSSMYAMMAYAVIQLIDPMWLDGLVFLPLIIAGLECLVDDGRKINYAIPLAFMFVAHFYIGFMIAIFIVIYFIFYLFFGTDRKGRKAYDYFQTIGRMCYTTIAGLLCSAIMLLPMVYTLSQGKMEFTESVWTQLSNIFHGDGDKLQQLKELFTAQFELIDLIPQLLPAQYDSVNVQGSPEIYCGLLTVVLLPIFYISNQIKLRKKIGYTFVLVCMVVSMYFKPIDMMWHGGQSPNWLPYRYSFLISFVLLTMAAMAFTKLKSVKPGILGAVFFGWMVLLLVISKLGYEHIDTIKSIWVSIILIGIYCVCLYFMKGGTKEDLKRVSNIAVTVVMLFAVGSELTYNAVDSMKKIDDEVAYSTKKSWTNFIENGRAATDQLEENDSGLYRAEKTFYRCVNDNGALGLRGISHSSSVMNTDIINFIETMGYCMHSYYTRYDGNTPLADSLLGIKYVLNRESDSTNRKLNPTYVAKPDWDYNYTDENNVSQTIRTYENPNALSIGYMADADVERIDHLGNDNPFNSQNMLMSVISGNMEFDASGAISGSKNYYSPVELDGDPILSNVTTSAYGDQTCYTAGQSGDPTVDFHLTVATEDPIYIFFKTENQKSVNLWLGQWNDETSDYDFDWFNAYFEGDNYTIMRLGQFDIGTKLCLRMTVANEYTIVKNFFFYSFDEAMFQEDIDKMKENQWNITKFNDRKITGTITAEEGQVMMTSIPYEDGWTVKVDGKKVEPVKLLNALVGVPLEPGEHEVTMTFTPPGWNIGLICLAAGIVVLILFYRYDKKHNAVLLAIAREKRQAADGKQPEPAKAVSAKPTTSTKSESEKTEKTEKTSKNAEKKESKPLAEEKPKESEAEDAADQKSDDSDSKDEKATDSDSESESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYH3</accession>
    <name>R5UYH3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Mutator mutT protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00863</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the Nudix hydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000051">
        <property type="protein sequence ID" value="CCZ83075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00863"/>
    <dbReference type="EnsemblBacteria" id="CCZ83075">
        <property type="protein sequence ID" value="CCZ83075"/>
        <property type="gene ID" value="BN566_00863"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="3">
        <location>
            <begin position="29"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="167" mass="19394" checksum="F0D54B68EFFE2D83" modified="2013-07-24" version="1">MEQWDIYDENKQKTGRTMNRNDWNMQPGDFHLTVLGVLCRPDGTYLITRRRMDKEWAPGCWEVPGGGVRAGESAEDAVKREILEETGVDVSNADGGYVFSYKRVNPEEKNNYFVDIFRYTMDIPDSAVHTQEREVMEYAFATLEQIQEYGKQGVFLHYDSIKQVFTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYL8</accession>
    <name>R5UYL8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Bifunctional protein PyrR</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="5">Pyrimidine operon regulatory protein</fullName>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="5">Uracil phosphoribosyltransferase</fullName>
                <shortName evidence="5">UPRTase</shortName>
                <ecNumber evidence="5">2.4.2.9</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="5" type="primary">pyrR</name>
        <name evidence="7" type="ORF">BN566_00912</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Also displays a weak uracil phosphoribosyltransferase activity which is not physiologically significant.</text>
    </comment>
    <comment type="function">
        <text evidence="5">Regulates transcriptional attenuation of the pyrimidine nucleotide (pyr) operon by binding in a uridine-dependent manner to specific sites on pyr mRNA. This disrupts an antiterminator hairpin in the RNA and favors formation of a downstream transcription terminator, leading to a reduced expression of downstream genes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>diphosphate + UMP = 5-phospho-alpha-D-ribose 1-diphosphate + uracil</text>
            <dbReference type="Rhea" id="RHEA:13017"/>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.9"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer and homohexamer; in equilibrium.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrR subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.9" evidence="5"/>
    <dbReference type="EMBL" id="CBAR010000060">
        <property type="protein sequence ID" value="CCZ83125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00912"/>
    <dbReference type="EnsemblBacteria" id="CCZ83125">
        <property type="protein sequence ID" value="CCZ83125"/>
        <property type="gene ID" value="BN566_00912"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004845">
        <property type="term" value="F:uracil phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription, termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01219">
        <property type="entry name" value="PyrR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023050">
        <property type="entry name" value="PyrR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2 5" id="KW-0806">Transcription termination</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pribosyltran" evidence="6">
        <location>
            <begin position="3"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="PRPP-binding" evidence="5">
        <location>
            <begin position="93"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ83125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="170" mass="19096" checksum="6A6BD7E31D4014EC" modified="2013-07-24" version="1">MKTIMDASAIQRAITRITYEILERNHGADQLAVIGIYSRGVILAKRIVERIAELEHRQVELGALDITTYRDDKVVPNHAEKTKIDFSVQDKKIILVDDVLYTGRSCRAAMDAVLERGRPTSIQLAVLIDRGHRELPIRPDYVGKNLPTAQTETVKVSVNELDDADCVTIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYU2</accession>
    <name>R5UYU2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Carbohydrate ABC transporter substrate-binding protein CUT1 family (TC 3.A.1.1.-)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00993</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000073">
        <property type="protein sequence ID" value="CCZ83210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00993"/>
    <dbReference type="EnsemblBacteria" id="CCZ83210">
        <property type="protein sequence ID" value="CCZ83210"/>
        <property type="gene ID" value="BN566_00993"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="97" mass="11149" checksum="422670460497BB6D" modified="2013-07-24" version="1">MSGVDSGVLLYENTKSMPSTYAAYDQITTFEEDENYKALYEQLRDYGHPRPKTPVYPQVSTSFQQVLEDVGLSGKDVQTEMDQSVERINAKLERYTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYZ4</accession>
    <name>R5UYZ4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Stage 0 sporulation protein A homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_01277</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000119">
        <property type="protein sequence ID" value="CCZ83508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01277"/>
    <dbReference type="EnsemblBacteria" id="CCZ83508">
        <property type="protein sequence ID" value="CCZ83508"/>
        <property type="gene ID" value="BN566_01277"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007492">
        <property type="entry name" value="LytTR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04397">
        <property type="entry name" value="LytTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00850">
        <property type="entry name" value="LytTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50930">
        <property type="entry name" value="HTH_LYTTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Response regulatory" evidence="6">
        <location>
            <begin position="3"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="HTH LytTR-type" evidence="7">
        <location>
            <begin position="133"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="5">
        <location>
            <position position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="244" mass="28829" checksum="1E76B36F6EE52999" modified="2013-07-24" version="1">MIQIAIVDDESRQIKIIQQSIQNFFRQKNRADDYRIDTFHSGESLLSCSRAYDLIFLDIQMNGMDGIETAKKIRTWDRKVCVIYVSNYTEKMAASFTVHPFSFLPKPIRQSDIYKNLEDFLLYTQPEEPKTRLVFQTLEEQISVNMEDIFYFEYGQNRRVKLVATTGTWYLSSSMKSLEKQMQSYGFLVPHKSFLVNPRQILSFGAEIEMKNHEMIPIAKNRRKQVHEQISAYLHNSLNINWRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZ00</accession>
    <name>R5UZ00_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC-type multidrug transport system ATPase and permease components</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN566_01282</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000119">
        <property type="protein sequence ID" value="CCZ83513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01282"/>
    <dbReference type="EnsemblBacteria" id="CCZ83513">
        <property type="protein sequence ID" value="CCZ83513"/>
        <property type="gene ID" value="BN566_01282"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="PTHR24221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="93"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="169"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="280"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="44"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="370"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="403"/>
            <end position="410"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ83513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="610" mass="67890" checksum="6357878FBBD28262" modified="2013-07-24" version="1">MANEQRRGMGRRMGGRMTVEKPKDFKGSIGKLFAYMKQYHAAVLIVMLCAVASTVFSVIGPDILGKATTELSNGLLAKIQGTGNINFTKIGQILLFVLGLYLLSSLFNFVQGWIMSGVTQKICYRMRKDISEKINRMPMAYFESRTYGEVLSRITNDVDTLGQSLNQSITQIITSVATMVGVLVMMLRISPLMTLIAVVILPISVAMISFVVKKSQKYFKQQQEYLGHINGQVEETYAGHTVIQAYNKEQDTIQQFSETNDTLYHSAWKSQFLSGLMQPIMMFVGNLGYAAVALSGGLLAIRGTITIGDIQAFIQYVKNFTQPMQQIAQVLNQVQSMAAASERVFAFLEEEEEVPTEHPIALPEQIAGTVSFQHVSFGYQPEQQIIHDFSADVQSGQKIAIVGPTGAGKTTMVKLLMRFYDVNSGAILLDGHNVKDFERHDLRKAFGMVLQDTWLFQGTIMENIRYGRLDATDEEVIAAAKAAHADSFIRQLPDGYQMELNEDASNVSQGQKQLLTIARAILADNRVMILDEATSSVDTRTEIQIQKAMDHLMEGRTSFIIAHRLSTIRNADLILVMKDGDIIEQGSHDELLAKNGFYADLYRSQFAQTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-09-29" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZ03</accession>
    <name>R5UZ03_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01287</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000119">
        <property type="protein sequence ID" value="CCZ83518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83518">
        <property type="protein sequence ID" value="CCZ83518"/>
        <property type="gene ID" value="BN566_01287"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="SSF51126"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="610" mass="66193" checksum="6B64732A048DCECB" modified="2013-07-24" version="1">MQITIEEATYYAVTDCRILAIQGEANTRSVHFIYPAVSGAEQYFLRVQLPESLIYDLPITENTVVLPASVTGYAGIVPIQWIAKNSTGSVIAKSQLIPSIVKPSIQGDTSALPTPEELITKYDEYLNTMRDIQQDVTKKSETVKADADRAESCVSRAEALLQSMQGLTIGERVRLYVDAEAGSDTNNGKATTTAVQTIDRALVLANAFQQAIICLKRGQTYTATDKSNEYGMGIQLYHKTLRFEAYGTASNKPKIQNAIFAYNCNFEWKDIDLTGNNSVFTMYNTISSFEGCAFYWVESHNSFAQVRLCDIQREWVQYGGFSVISNAESLYHHIGGIQVNQGARVDYSGFGIGSYGNDGCSVLTIDAIPEKLQKTKNIAEGSIETKTVYVNAATGSDARDGTSQAKALLTMSRALQFTQYAKKAVIYLAAGTYPIPNKTLTLLGRDVRIYGNAAATTTIQGNFVCENGFLHLSKVTIDNTDSDTANTSTTAILAQYNGTVRISDCVVNANSKNAVGVSDMSNICCSGTEFKGNAQYAVYVTGQGDAKIYSCTNSTTKSIYSGANSMVRITQSNESDFPYTNANNGMVFVNGQQVLPKTTAVTSNQATSSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZ06</accession>
    <name>R5UZ06_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Cysteine synthase</fullName>
            <ecNumber evidence="5 11">2.5.1.47</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN566_01066</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>hydrogen sulfide + O-acetyl-L-serine = acetate + L-cysteine</text>
            <dbReference type="Rhea" id="RHEA:14829"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:58340"/>
            <dbReference type="EC" id="2.5.1.47"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 9 11">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the cysteine synthase/cystathionine beta-synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.47" evidence="5 11"/>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01066"/>
    <dbReference type="EnsemblBacteria" id="CCZ83285">
        <property type="protein sequence ID" value="CCZ83285"/>
        <property type="gene ID" value="BN566_01066"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00136">
        <property type="reaction ID" value="UER00200"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004124">
        <property type="term" value="F:cysteine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005856">
        <property type="entry name" value="Cys_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005859">
        <property type="entry name" value="CysK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001216">
        <property type="entry name" value="P-phosphate_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01139">
        <property type="entry name" value="cysK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01136">
        <property type="entry name" value="cysKM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00901">
        <property type="entry name" value="CYS_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8 11" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="7 9 11" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PALP" evidence="13">
        <location>
            <begin position="11"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="143"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="9">
        <location>
            <begin position="181"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="10">
        <location>
            <position position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR605856-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR605856-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CCZ83285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="310" mass="32850" checksum="6076022F21E37FBC" modified="2013-07-24" version="1">MANIYKGTIELIGNTPLVEAVNLEQAHDLQATLLIKLEYLNPAGSVKDRIAKSMLEDAEQSGKLHTGSVIIEPTSGNTGIGLAAIAAAKGYRVILTMPETMSVERRNILKAYGAEIVLTEGAKGMKGAIAKAKELAEEIPNSFIPGQFENPANPEAHRKTTGPEIWRDTDGKVDYFVAGVGTGGTITGVGEYLKSQNPNVKIVAVEPETSAVLSGEKPGPHKIQGIGAGFVPKTLNTGVYDEIIKIQNDDAFTTAKELAKHEGILVGISSGAALQAALELAKRPENKGKTIVALLPDSGDRYYSTTLFSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZ45</accession>
    <name>R5UZ45_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Recombinase/Resolvase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00133</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000135">
        <property type="protein sequence ID" value="CCZ83588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83588">
        <property type="protein sequence ID" value="CCZ83588"/>
        <property type="gene ID" value="BN566_00133"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038109">
        <property type="entry name" value="DNA_bind_recomb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011109">
        <property type="entry name" value="DNA_bind_recombinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025827">
        <property type="entry name" value="Zn_ribbon_recom_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07508">
        <property type="entry name" value="Recombinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13408">
        <property type="entry name" value="Zn_ribbon_recom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00857">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="SSF53041"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51737">
        <property type="entry name" value="RECOMBINASE_DNA_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="1">
        <location>
            <begin position="26"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Recombinase" evidence="2">
        <location>
            <begin position="183"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="518" mass="60264" checksum="A95CE36C844C1390" modified="2013-07-24" version="1">MREVTVIPRKPRVGNTATKIEVKKLRVAAYCRVSTDTEEQASSYETQISHYEESIRSKPEWELVNVYADDGISATSTKKREEFNRMIEDCKKGLIDIIITKSISRFARNTVDCLNYIRMLKDMNIPVYFEKESINTMDAKGEVMITIMASLAQQESESLSQNVKLGMQYRFQQGIPMINTTCFLGYDKDKNGNLVINPKEAEIVRRIFREYLNGSSCQAICKGLERNGIKTSRGNKRWHDTTVRKILENEKYMGDLLLQKTYTIDFLSKKRVKNNGDMPQYYVESNHEPIVSRETFMLVQEEIARRGMLRDCQGRRRGYSSKYCMTGITYCANCNERYKRIIWNIHGRKTPVWRCSSRLHDHNSCSARSIHEDKLQKAFVAALNQMIGDSGEYLQILQDNLEEALTVGKSANILKIDEQLRKLQQELTIRTENHQGYDDIAEEIFRLKEQREQLMMDETTRSDYKERINDLKKFIATSYHSITEYDETLARHMLSKITIFDDHIVFAFKSGVIVSVEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZ63</accession>
    <name>R5UZ63_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CttA_N domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00140</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000146">
        <property type="protein sequence ID" value="CCZ83613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00140"/>
    <dbReference type="EnsemblBacteria" id="CCZ83613">
        <property type="protein sequence ID" value="CCZ83613"/>
        <property type="gene ID" value="BN566_00140"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040959">
        <property type="entry name" value="CttA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18244">
        <property type="entry name" value="CttA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004394818" description="CttA_N domain-containing protein" evidence="2">
        <location>
            <begin position="30"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="domain" description="CttA_N" evidence="3">
        <location>
            <begin position="541"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="434"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF18244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="734" mass="77671" checksum="56A13E84D4EB90C4" modified="2013-07-24" version="1">MKKVFNKVIAVAATVPLALTQGFAVLSNAADATTISVERVLEIKPQELESDWAKLVEGALIDSEGKSFELNVADIIDSADFSDKNAAYVEKVQKCIVGNPSVSIQNSVAVMTAQVDATSYVQSNIIDAIKKELTKQGAASIIDDIDFSMLEKKGTFTAAVDGDSIENTAANKAAGTANTIRVKGAVFVDSEGNQVATYADAKAYAASTVQKLKVELTKKAAAAGVEVNLDSLLATYQKKVEKAFSYADKAITTSAHQEFATADETLAWLKVQKDQRTTRFDVPGSVADLAKYGDTLNKAVAEVNKAFQNVGVNYTVDVSMDDVTSLLNSGSDFVADANADTNVYTVTFKVPDTDVDADYFSTVEGIEKYYAAHPDQAETDGVTDADYSQLVYDSSYKLVTAQGDADNDTLAGVPSGYFNVERIIKFKDKATTTTTTDISGTTTTDVSGTTTTDVSGGTTTTDVSGTTTTDVSGGTTTTDVSGTTTTDVSGGTTTTDVSGATTTTTTGTGDSETTTTTTTTNTGTITPVGTTTLEVEVNPTNFYFSVDERELKPSDLFSKFDIVDEDGTHFNALELGAVTLDKTTPKEIFDAEGKPYCVTEVNAYFTDPTKADAEPVAAPVNPTVYIGVKGDTDLSGLVDVPDAVAVLTYTAKIAAGQEGVVFNEDENLNKFVFFLADIDTESKEGKNDGNKLMATEDAVYILTYTAKTAAGQEPTWPDVIPSLKSLEGSIWYEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZQ0</accession>
    <name>R5UZQ0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01249</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000116">
        <property type="protein sequence ID" value="CCZ83480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83480">
        <property type="protein sequence ID" value="CCZ83480"/>
        <property type="gene ID" value="BN566_01249"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="275" mass="31502" checksum="0D625A34F2C9DFA1" modified="2013-07-24" version="1">MVNAADVGINLNLCAVFGDVFLALEFLQQAFLFQILLLLFEQPSLCFFVWVYENFSRKAVYNGFHTVLNCLQECIAHADNCWNIHVSCKNRRVRVGRTVCGDKRQHFIFIQRNGFTRGEVVCTNNHAFLGCGCTGDVSGQVCNDAVRNVFYIRCSCLHICIIHRRKHLCKVVRRHSNRIFCIDFLGFNDVGNAFVVILIFQHHHVNLENLSVGFADFFQCLFIDCLQLFTGSRLCSFDSCLFRLGIFHVHPLNRDIILLKNGEFSNGNRSINTFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZZ5</accession>
    <name>R5UZZ5_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Dockerin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01314</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000121">
        <property type="protein sequence ID" value="CCZ83545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83545">
        <property type="protein sequence ID" value="CCZ83545"/>
        <property type="gene ID" value="BN566_01314"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002105">
        <property type="entry name" value="Dockerin_1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00404">
        <property type="entry name" value="Dockerin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004394532" description="Dockerin domain-containing protein" evidence="1">
        <location>
            <begin position="35"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="2">
        <location>
            <begin position="241"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="328" mass="35640" checksum="3E3B93A1460687F6" modified="2013-07-24" version="1">MQLFQTISRKSLSIWLSLLCMLSLWSSFSLSASAEDTIPFPIAWQQSEGSGAEEQRLAYLPEVDNWCDYPWNRGTETGNTVGQAGCSLLSIVNAVYYRTGSFLHPAKLAQFALDNGYRIPGVDGAAMGFFPAVAQAYAEESHMTFAGWTTQAAAVLEHLRNGGTASANIAGHWIALVDYDVTTDQYLMLDSSQISSRAEHIAWTDRENGVAWLPEAVLLENGRNGYYGINNRYSALYTFDDPYTLGDVNQDGVVSVEDARLVLQYYASTAASLDFRLHPQIVTHNAGISAADIDGDGIVTVQDATAILSYYAQQASGAAPSWGQVLCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>R5V027</accession>
    <name>R5V027_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00135</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000135">
        <property type="protein sequence ID" value="CCZ83590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83590">
        <property type="protein sequence ID" value="CCZ83590"/>
        <property type="gene ID" value="BN566_00135"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038109">
        <property type="entry name" value="DNA_bind_recomb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011109">
        <property type="entry name" value="DNA_bind_recombinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025827">
        <property type="entry name" value="Zn_ribbon_recom_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07508">
        <property type="entry name" value="Recombinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13408">
        <property type="entry name" value="Zn_ribbon_recom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00857">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="SSF53041"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51737">
        <property type="entry name" value="RECOMBINASE_DNA_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="1">
        <location>
            <begin position="17"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Recombinase" evidence="2">
        <location>
            <begin position="173"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="514" mass="59288" checksum="C7DBFC6DC46A9E7A" modified="2013-07-24" version="1">MIVRKIEAKKQKQTLCRVAAYCRVSTDNHDQLESLETQKEHYESCIRAHADWECAGIYYDSGISGTSSDTRDGLQALLQDCRNGKVNYILTKSISRFSRNTADCLSIVRELIRLQIPIYFEKENLNTGTMDSELILSILSSMAEEESISISKNLKWSIQRKFRNGTFKFSCVPYGYTRNADGEMIIEPQEAKIVKRIFKMVVSGLGSHRIAKKLNADGIIPRKGANWTSTSILNIIGNEKYMGDALFQKTFTTDSFKRKKNHGEMESFLIHDHHEPIISRELFDQAQETIRWRARNHQKGSGIYENRYCFSGKIFCNCGSVMKRQSNNRISWCCITHINDISHCDMKSIRDDSLKTAFVTMINKLIYAKGILLKPYYDSVAASAGDENVIHIRELQEKIKLLSVRTDNLRKLRAQNMIDNVVFNRELNDMKKQEDLYRMEMMQCSNAESQGAILLRETEKLMKLIDHSEMLTAFDEEQFTEYVDKIVITDRKTAVFYLKCGLKLKEELQCMATE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5V068</accession>
    <name>R5V068_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">TIR-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01381</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000147">
        <property type="protein sequence ID" value="CCZ83645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83645">
        <property type="protein sequence ID" value="CCZ83645"/>
        <property type="gene ID" value="BN566_01381"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019302">
        <property type="entry name" value="TIR-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10137">
        <property type="entry name" value="TIR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TIR-like" evidence="1">
        <location>
            <begin position="111"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="253" mass="29063" checksum="0A413121F8A205CE" modified="2013-07-24" version="1">MGNYEKLKGICLQIDKLIALKVVRSSNEFQIWLKNTRRFLCNCFGENSIELREFNKLHFDLALVNVENWERVEKCKNDLEIAKGMFENYLEDMESDTTKDTDSIKDEEFNKVFIVHGHDTNLKLEVARLLENQGIEAIILHEQVNAGMTIMEKIESYGNTVNAAIILFTPDDDGKAKKEEVLKNRARQNVVFEAGYFAGLLGRNRTIFIVSDDSMELPGDLNGIIYNKESWQFKVIKELKGIGFNVDANKLMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>R5V077</accession>
    <name>R5V077_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6 7">Elongation factor Ts</fullName>
            <shortName evidence="6">EF-Ts</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">tsf</name>
        <name evidence="10" type="ORF">BN566_01717</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6 7">Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF-Tu.GTP complex up to the GTP hydrolysis stage on the ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the EF-Ts family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000178">
        <property type="protein sequence ID" value="CCZ83988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01717"/>
    <dbReference type="EnsemblBacteria" id="CCZ83988">
        <property type="protein sequence ID" value="CCZ83988"/>
        <property type="gene ID" value="BN566_01717"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00050">
        <property type="entry name" value="EF_Ts"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036402">
        <property type="entry name" value="EF-Ts_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001816">
        <property type="entry name" value="Transl_elong_EFTs/EF1B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014039">
        <property type="entry name" value="Transl_elong_EFTs/EF1B_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018101">
        <property type="entry name" value="Transl_elong_Ts_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11741">
        <property type="entry name" value="PTHR11741"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00889">
        <property type="entry name" value="EF_TS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="SSF46934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54713">
        <property type="entry name" value="SSF54713"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00116">
        <property type="entry name" value="tsf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01126">
        <property type="entry name" value="EF_TS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01127">
        <property type="entry name" value="EF_TS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6 7" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="4 6 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF_TS" evidence="9">
        <location>
            <begin position="73"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Involved in Mg(2+) ion dislocation from EF-Tu" evidence="6">
        <location>
            <begin position="82"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ83988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="307" mass="33026" checksum="37BA96B594B54AA6" modified="2013-07-24" version="1">MANFTAKDVNELRKNTGVGMMDCKKALTEADGDVEKAMLILREKGLATQAKKAGRVAAEGMVIAKVNADHTVGCVVEVNSETDFVANTDEFKAFVNTVADTIIEKNPADVEALKSTVIAGGTATVDEALQELFLKIRENLQIRRFTRLEGILVPYIHGGGKIGVLVQAASPAGASEAVLTAAKDCALQIAAMKPSYLKREDVPATVLEEEKTVVLAQMAEDPKMKTKPQQVLAKIAEGKVGKYYSENCLLEQTFVKDGSMNVKQYVDSVAKAAGTTIDITGYVCFERGEGIEKKADNFAEEIASMIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0I4</accession>
    <name>R5V0I4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator XRE family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01515</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000161">
        <property type="protein sequence ID" value="CCZ83780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01515"/>
    <dbReference type="EnsemblBacteria" id="CCZ83780">
        <property type="protein sequence ID" value="CCZ83780"/>
        <property type="gene ID" value="BN566_01515"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="6"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="73" mass="8903" checksum="2DAD898B9CCA6857" modified="2013-07-24" version="1">MRYQRIRDLREDHDLYQKDLADYLHCSQVAYSYYELGKRDIPTSILIQLANFYHTSIDYLLNQTDEKQPYPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0K0</accession>
    <name>R5V0K0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">D-lactate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_01530</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000164">
        <property type="protein sequence ID" value="CCZ83795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01530"/>
    <dbReference type="EnsemblBacteria" id="CCZ83795">
        <property type="protein sequence ID" value="CCZ83795"/>
        <property type="gene ID" value="BN566_01530"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00670">
        <property type="entry name" value="D_2_HYDROXYACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2-Hacid_dh" evidence="4">
        <location>
            <begin position="4"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="2-Hacid_dh_C" evidence="5">
        <location>
            <begin position="108"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ83795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="328" mass="36819" checksum="5C8730FA6338CFF8" modified="2013-07-24" version="1">MEQIAFFDTKPYDRTWFDQLNHRFRIRYFEHKLTPDSVSLAQGCSAVIPFVNDNLSAPVIEDLYRFGVKVIALRCAGYNNVDRNAAHGKMPILRVPGYSPYAVAEHTMGLLLCLNRKIHKAYNRTRDFNFTLNGLTGFDLHGKTVGVIGTGRIGQIFMNICKGFGMEVLAYDPYPANDPSIHYVDLHTLFQQSDILSLHCPLTEQSKHLINKDTIADMKDGVVLLNTSRGMLIESEALLSALQSGKIAAAGLDVYEEETAFFFEDFSENIHRDQVLSLLLSMPNVLLTSHQAFLTNEALKNIAEVTLQNLADFFDGKPLQNEVKTGQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0K9</accession>
    <name>R5V0K9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01854</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000189">
        <property type="protein sequence ID" value="CCZ84128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01854"/>
    <dbReference type="EnsemblBacteria" id="CCZ84128">
        <property type="protein sequence ID" value="CCZ84128"/>
        <property type="gene ID" value="BN566_01854"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031308">
        <property type="entry name" value="UCP028777"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005185">
        <property type="entry name" value="YccF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03733">
        <property type="entry name" value="YccF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028777">
        <property type="entry name" value="UCP028777"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="YccF" evidence="2">
        <location>
            <begin position="4"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="YccF" evidence="2">
        <location>
            <begin position="66"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="119" mass="12717" checksum="95BE7F1BB7C4F879" modified="2013-07-24" version="1">MSCLGNLLWFLCCGLWQGLSWLLAGLLWCVTIVGIPIGLQCFKFASLAFFPFGKEVRYGGGTVSLLLNLIWILVSGIPLAVTAAANGILLCCTIIGIPFGLQCFKMAKLALMPFGAEVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5V120</accession>
    <name>R5V120_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dockerin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01689</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000176">
        <property type="protein sequence ID" value="CCZ83960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83960">
        <property type="protein sequence ID" value="CCZ83960"/>
        <property type="gene ID" value="BN566_01689"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002105">
        <property type="entry name" value="Dockerin_1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032267">
        <property type="entry name" value="DUF4832"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032379">
        <property type="entry name" value="DUF4874"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00404">
        <property type="entry name" value="Dockerin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16116">
        <property type="entry name" value="DUF4832"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16173">
        <property type="entry name" value="DUF4874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="SSF48726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00448">
        <property type="entry name" value="CLOS_CELLULOSOME_RPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="1" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="1" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004394709" description="Dockerin domain-containing protein" evidence="2">
        <location>
            <begin position="27"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="3">
        <location>
            <begin position="766"/>
            <end position="832"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="837" mass="93390" checksum="08485E7C4DA2CBF7" modified="2013-07-24" version="1">MKRKKIPILLLTAVCCLAMLPTAAVATTAAEPATQTETTLSDTELVDSGISYVETVETIANPGAGYTTTDWYTCAPGNTPIHDKNGSVVLMFIDIGKFSSGANGTTNEDGTYVEGTDYDLDEQFFQNVRASFENCRKNGSTIAVRFRYDANGKDNPEPATFDQVLRHIQQIKENGLLEDYKDILMFVETGFVGKWGEQHGGKYTSLDYKVQLVNAMLDCVPKEVPITVRTPNIFEKWAGITTEEMATYVAEPGSDAARIGLYNDGYMGSDSDLGTYSNRTAQTAWMEKQMEHTYYGGEFSGNLDWAKKYDTYLPENAIPEMYRTHLSYINSNIYQLYKDYTFGAEYDVENGDHSAYYGQTVFQFIRDHLGYRFVIRDSKLSKQVTAGDTLQCQLSIENTGFANPIRKMKAEVLLEKDGDYIQTTIDIDPTTWLSCTTTDIPLTIQLPGDLEAGNWNVYLRLSIGNQDIPDSTLRTVHFANADVWDGMLGANRIGTVAITAATDAEQLTQHDFYQVNTETPALSDGTRYNRNAVVSIDGKRSGAAEWRTDALYHTEDNANLYLTNDDQYLYVMAEMPMDEVVSPVYNLQVIHADPENPTENKKYWMYHMRNGYVYFNQGSYAGTICKYTGSCVEWKIPFSVLELAPGTELTSVRVTIQDSSVDGWKKTSDQEIDRPYVVTSTFPTYNACRSMVLKEDESISLSTVTTLTGATYQWYHDDVAIPDATASSYMISDATADTIGSYTVEITAPSGTKKTLSICNVTDVLSSVKMGDVNGDASVDLLDVVLLQKYLIWKTDGFEINIQQADCQLDEKLDVLDLVVLKRMLFPPSEPETEQGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1D5</accession>
    <name>R5V1D5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Myosin-crossreactive antigen</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01781</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000184">
        <property type="protein sequence ID" value="CCZ84055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01781"/>
    <dbReference type="EnsemblBacteria" id="CCZ84055">
        <property type="protein sequence ID" value="CCZ84055"/>
        <property type="gene ID" value="BN566_01781"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050151">
        <property type="term" value="F:oleate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010354">
        <property type="entry name" value="Oleate_hydratase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37417">
        <property type="entry name" value="PTHR37417"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06100">
        <property type="entry name" value="MCRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="597" mass="68434" checksum="78725CD32167D343" modified="2013-07-24" version="1">MYYSAGTYESFAHPEKPKDVDKKSAYIIGTGLAGLTAAFYLVRDGQMKGEHIHLFEKLELAGGSCDGRKDVTKGFFMRGGREMDNHFEVMWDTFRDVPSIETPGISVLDEYYWLNKHDPNYSLCRATVNCGKDAHTDKKFTLDKESALALSKLFLTPEKDLENKKISDVLPNSFWKTNFWLYWQTMFAFQRWSSALEMKRYLCRYVHHIDGLPDFSALRFTKYNQYESMILPLVKYLEAHGVQIEYGMDVKNVIIETIGTKKVAKQIIYRKDGPDQTIDLIEDDLVFITNGCCTDTSCYGDQTHAPDLSHIKNGCGESWDLWKAIADQAKHGEFGKPEVFCGNVNATNWMSATVATSNEEIIQHIIRVCKRDPRAGKVTTGGIVTVKDSTENWYLSWTINRQPQFRSQDKNMVLVWLYGLHTNRAGNYVKKAMRNCTGEEICQEWLYHIGIPKEKIPELAKNACNTTTCFMPYINAFFQPRKESDRPRVVPNGAVNFAFIGQFAETPRDTIFTTEYSMRTGMESVYTLLDIDRGVPEVWGSKYDVRELLRACYYAIDKKPITDVPLSFVEKKMIRVLLKKVHGTDIELMLKESGLIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1P3</accession>
    <name>R5V1P3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_01925</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01925"/>
    <dbReference type="EnsemblBacteria" id="CCZ84200">
        <property type="protein sequence ID" value="CCZ84200"/>
        <property type="gene ID" value="BN566_01925"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007525">
        <property type="entry name" value="FrhB_FdhB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007345">
        <property type="entry name" value="Polysacch_pyruvyl_Trfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04432">
        <property type="entry name" value="FrhB_FdhB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04230">
        <property type="entry name" value="PS_pyruv_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="6"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="40"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ84200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="914" mass="102383" checksum="9FB44F855FAAC5BF" modified="2013-07-24" version="1">MIATTSLPQLPGKTCTGCGGCTNACPKDAIFFAPDAEGFQHPVVQAAQCIGCKKCEQICPVLHWESSNIAKPDLYAVRGADDIRANSSSGGVFSLAAEEVFARKGVVVGAAFDKNLHLSLSIAKNEKQLAPMRGSKYVQSDPELVYRDALKCLKAGKLVLFTGCPCQVAAMKNIAGNKYNDLLYTIDLLCHGVPSQAILQRYLEETFPGKQAVDVRFRDKSHGWIATYIDVDFSDGTSYHGANRRNDPYERIFQHNIALRPCCHNCKFCTFPRTGDLTMGDFWGIQKIDPSQTDGKGTSMVFLNNEKGRQLFTWFQPQTDSKQMHVNLATIPNRLKAAYPPHPGRSVFFPLLRTKSMEEIVKMVMDNQHDIGLVGIHTVGNFGGALTYFALYKVLCDLGYSVLMIERPGNCAHKPALTKIYGTSPYPAFDTAKIYPNKQAMKELNQFCKMFVVGSDQLFNDGLYNNFGRWCTLDWVQDNKKKIAYAASFGHDYIWSPEETRAEMAHFMKRFDAFSVREQSGVDICKKEFDIDAQWVLDPVFLCKPEYYRELAKQSTHPMEENYLGAYILDPTKEKADILRYAAKKKKLSTTVYTEMAYQQKDLNDFDLPVQFGGLIEDRLANIIHSDFFIADSFHGICFAILMKKNFVAIVNEKRGKSRFESILGLLGLKDRLISNLTELKQKEALLTTPVDYDAVYEILEQERHRCLTWLRDALADPTKKPLSTYDLLTRRIDLLSERVSKLMIANATANRKIAHLVEFAGFSLTAISDFSKYFKALQANIENYIIAIAVKDTPGLCFTDALYADMQRIGVTVNLTKKHWYGYAAIIDGGKLLAEDAAYQKVISVKATTEDGVAVVATSKPLKVGNATAISFNGIGRSVSRRGINITVYDKTKKCVCDSVCFDTHVKSIDCHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1S3</accession>
    <name>R5V1S3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Pseudouridine synthase family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00179</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000195">
        <property type="protein sequence ID" value="CCZ84240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00179"/>
    <dbReference type="EnsemblBacteria" id="CCZ84240">
        <property type="protein sequence ID" value="CCZ84240"/>
        <property type="gene ID" value="BN566_00179"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120159">
        <property type="term" value="F:rRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1560">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042092">
        <property type="entry name" value="PsdUridine_s_RsuA/RluB/E/F_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000748">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluB/E/F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020094">
        <property type="entry name" value="TruA/RsuA/RluB/E/F_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00093">
        <property type="entry name" value="TIGR00093"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S4" evidence="2">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="250" mass="27937" checksum="9BC892FDB1154E7E" modified="2013-07-24" version="1">MRLDKWLSDATSYTRSQLKQLIRSGQVTVNQTIVKKPETPVQETDVICLQGNPVEPAESCYCLLLNKPLGYVCAVTDATEKTVMELIPPELQRKGLFPVGRLDKDTSGMLLLTNDGAFSHALLAPKRHVPKYYRMQLARPYEESYAQQLAEGLVLADGTHCLPAEILPVLNTDRMAVICLHEGKYHQVRRMLAAVGNHVEQLQRVAIGNLFLPIGLQIGACQKLTSTQISLLSQQPDPKFLWELTQNSNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>R5V222</accession>
    <name>R5V222_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Gamma-glutamyl phosphate reductase</fullName>
            <shortName evidence="7">GPR</shortName>
            <ecNumber evidence="7">1.2.1.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Glutamate-5-semialdehyde dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Glutamyl-gamma-semialdehyde dehydrogenase</fullName>
            <shortName evidence="7">GSA dehydrogenase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">proA</name>
        <name evidence="9" type="ORF">BN566_00272</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the NADPH-dependent reduction of L-glutamate 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>L-glutamate 5-semialdehyde + NADP(+) + phosphate = H(+) + L-glutamyl 5-phosphate + NADPH</text>
            <dbReference type="Rhea" id="RHEA:19541"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58066"/>
            <dbReference type="ChEBI" id="CHEBI:58274"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.41"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the gamma-glutamyl phosphate reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.41" evidence="7"/>
    <dbReference type="EMBL" id="CBAR010000220">
        <property type="protein sequence ID" value="CCZ84365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00272"/>
    <dbReference type="EnsemblBacteria" id="CCZ84365">
        <property type="protein sequence ID" value="CCZ84365"/>
        <property type="gene ID" value="BN566_00272"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00360"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004350">
        <property type="term" value="F:glutamate-5-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd07079">
        <property type="entry name" value="ALDH_F18-19_ProA-GPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00412">
        <property type="entry name" value="ProA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020593">
        <property type="entry name" value="G-glutamylP_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012134">
        <property type="entry name" value="Glu-5-SA_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000965">
        <property type="entry name" value="GPR_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000151">
        <property type="entry name" value="GPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00407">
        <property type="entry name" value="proA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01223">
        <property type="entry name" value="PROA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7" id="KW-0521">NADP</keyword>
    <keyword evidence="6 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 7" id="KW-0641">Proline biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldedh" evidence="8">
        <location>
            <begin position="31"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ84365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="419" mass="44863" checksum="428A925A164B3F08" modified="2013-07-24" version="1">MNYAQTLGERAKVAAPAIANASPKLKNEALAAIAAALLQQTDRILQANAEDLAAAVQNNMTESLQDRLRLTPARMQGMADAIEKLIAMDDPVGVVESGSVRPNGLQILKTRVPLGIIGIIFESRPNVTVDAATLCLKAGNAVILRGGKEAIHSNTCLMNVMREAVESVGLPADIMQLVEDTSRQTASDMMQLNGYLDVLIPRGGAGLIQAVVKQATVPVIETGVGNCHVYVDSCLDGDADYEMAVRIIDNAKTQRPSVCNAIEGLLVHEAVAEKLLPMVQKALAVHNVTIYGCEKTRAILGDSVLPATETEYATEFLDYKISVKVVSDLEEAIAHITKYGTKHSECIVTRSLNAAKKFQQQVDAAAVYVNASTRFTDGGEFGYGAEIGISTQKLHARGPMGLHELTTIKYLINGDGQIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>R5V252</accession>
    <name>R5V252_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">Glutamate dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN566_00466</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4 8">Belongs to the Glu/Leu/Phe/Val dehydrogenases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000257">
        <property type="protein sequence ID" value="CCZ84613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00466"/>
    <dbReference type="EnsemblBacteria" id="CCZ84613">
        <property type="protein sequence ID" value="CCZ84613"/>
        <property type="gene ID" value="BN566_00466"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004352">
        <property type="term" value="F:glutamate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:cellular amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01076">
        <property type="entry name" value="NAD_bind_1_Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminiacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006095">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033524">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006096">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006097">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_dimer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014362">
        <property type="entry name" value="Glu_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033922">
        <property type="entry name" value="NAD_bind_Glu_DH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00208">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02812">
        <property type="entry name" value="ELFV_dehydrog_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000185">
        <property type="entry name" value="Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00082">
        <property type="entry name" value="GLFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00839">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="SSF53223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00074">
        <property type="entry name" value="GLFV_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ELFV_dehydrog" evidence="9">
        <location>
            <begin position="183"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="351"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalysis" evidence="7">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ84613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="418" mass="45605" checksum="CB8321891EA0D863" modified="2013-07-24" version="1">MEKPYNPFENVQTVMEEAMEVGGIDRTMFEIIRNPQRETKVYLPVEMDDGSIHVFEGWRVQHSNIRGPFKGGIRFHQDVNLDEVKALATWMSLKCAVANIPYGGAKGGVRVDPHTLSKRELCRLTRRYTYAIEPLIGQDKDIPAPDVNTNAQTMAWVLDTYSMLKGKPCPGVVTGKPVELGGSKGRPSATGRGVVISTKLILGKHGKELAGTAVAIQGMGNVGSNAAKIFYHRNAKIVALSDVSGGIYCADGLDADAIAAYTGNGGLLKDYNGAGIQHISNRELLTCACDVFVPAALENQIDEEIAAEMQCKFIVEGANGPTTAAADKILEQRGIVLVPDIFANSGGVIVSYFEWVQNIQELTWEREQVNEMLEKLMTKAFAALYETAQEKHCTLRMGAYVVALQRLIDAEVIKGLFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5V2C1</accession>
    <name>R5V2C1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">FHA domain containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00546</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000267">
        <property type="protein sequence ID" value="CCZ84693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84693">
        <property type="protein sequence ID" value="CCZ84693"/>
        <property type="gene ID" value="BN566_00546"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="142" mass="15651" checksum="641AF8A2323CEEA5" modified="2013-07-24" version="1">MQQKWDSEEVQMKTCRNCGRVNSDTAQHCVQCGNLLQQQSDGVSTQNNSNSIPKKNSGLFRNAGAKLKVLALIFFIIHIVIGVAGGVTCFIIGANSYDGYIFVIIGLIAIFVSPLIGWLNAIILYAFGELCENVNDIRNQRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5V2V0</accession>
    <name>R5V2V0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00094</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000252">
        <property type="protein sequence ID" value="CCZ84600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00094"/>
    <dbReference type="EnsemblBacteria" id="CCZ84600">
        <property type="protein sequence ID" value="CCZ84600"/>
        <property type="gene ID" value="BN566_00094"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="SSF69318"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004394604" evidence="1">
        <location>
            <begin position="26"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="437" mass="48321" checksum="E94294DBC0C5B7D2" modified="2013-07-24" version="1">MRPKGLIRLLLTGCLLLLLSGCTFGATVDALLTPPHLRGEQEQIYQALENAVGNRITLQYPRSGDHLSAVMISDLDGDGQDEAAVFYRKESSLSTENALRLNLLDQADGTWMSVCDLPAEGAEVDRGFTADIGGRQRLIIGYSQVDQSDKALVVYNYEGGTLAKSFTVSYSSFDVADLDGDDQSELLILKAAEGEHAAEAAVYVPDAQDHYTRYSLTLQGKTTEYSQVLCGNTQGKQTAIYLDGIVGATTMQTEVLQFDGKKLSYLVPTGSDAVSTIRPIGWLTMDIDQDDIPEIPVQGLFPGYQDSDSDLIRMTRWMTVTKDGTLQEEERGYYSLGDGYAFLLPPNWQTEVTVQNDNLTGDLVFYHYMGDRAAEMPELMRISVATDTANRDSRLEEGYQLLYSRGNAYYFIKVPEDSTDERARTVGDLLGYFVFLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>R5V320</accession>
    <name>R5V320_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 13 15">tRNA (guanine-N(1)-)-methyltransferase</fullName>
            <ecNumber evidence="6 13 15">2.1.1.228</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">M1G-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">tRNA [GM37] methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">trmD</name>
        <name evidence="17" type="ORF">BN566_00528</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 13 15">Specifically methylates guanosine-37 in various tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13 15">
            <text>guanosine(37) in tRNA + S-adenosyl-L-methionine = H(+) + N(1)-methylguanosine(37) in tRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:36899"/>
            <dbReference type="Rhea" id="RHEA-COMP:10145"/>
            <dbReference type="Rhea" id="RHEA-COMP:10147"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="EC" id="2.1.1.228"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5 13 15">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 13 15">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13 15">Belongs to the RNA methyltransferase TrmD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.228" evidence="6 13 15"/>
    <dbReference type="EMBL" id="CBAR010000267">
        <property type="protein sequence ID" value="CCZ84675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00528"/>
    <dbReference type="EnsemblBacteria" id="CCZ84675">
        <property type="protein sequence ID" value="CCZ84675"/>
        <property type="gene ID" value="BN566_00528"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052906">
        <property type="term" value="F:tRNA (guanine(37)-N(1))-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18080">
        <property type="entry name" value="TrmD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1270.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00605">
        <property type="entry name" value="TrmD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002649">
        <property type="entry name" value="tRNA_m1G_MeTrfase_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023148">
        <property type="entry name" value="tRNA_m1G_MeTrfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016009">
        <property type="entry name" value="tRNA_MeTrfase_TRMD/TRM10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46417">
        <property type="entry name" value="PTHR46417"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01746">
        <property type="entry name" value="tRNA_m1G_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000386">
        <property type="entry name" value="tRNA_mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00088">
        <property type="entry name" value="trmD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 13 15" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 13 14" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="10 13 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="12 13 15" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA_m1G_MT" evidence="16">
        <location>
            <begin position="23"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="13 14">
        <location>
            <begin position="133"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13 14">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00014679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000386-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CCZ84675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="231" mass="26168" checksum="099C37ED94E4F1D2" modified="2013-07-24" version="1">MQIDIATLFPEMCDAVLQESILGRAQKSGAISVQCWNIRDYTLNKHKRVDDVPYGGGRGMVMQADPIYRCWEAVCAERESKPHCIYLSPKGSVFTQQKAVALSQLPHLFLLCGHYEGIDQRILDTIIDEEISIGDYVLTGGELPALVLTDAVARMCEGVLPDAVCFEEESHYNGLLEYPQYTRPEVWHEQAVPPVLLSGHHKNIETWRFAASLQETREKRPDLYAKYVDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>R5V329</accession>
    <name>R5V329_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Arginine biosynthesis bifunctional protein ArgJ</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="7">Glutamate N-acetyltransferase</fullName>
                <ecNumber evidence="7">2.3.1.35</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="7">Ornithine acetyltransferase</fullName>
                <shortName evidence="7">OATase</shortName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="7">Ornithine transacetylase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="7">Amino-acid acetyltransferase</fullName>
                <ecNumber evidence="7">2.3.1.1</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="7">N-acetylglutamate synthase</fullName>
                <shortName evidence="7">AGSase</shortName>
            </alternativeName>
        </domain>
        <component>
            <recommendedName>
                <fullName evidence="7">Arginine biosynthesis bifunctional protein ArgJ alpha chain</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="7">Arginine biosynthesis bifunctional protein ArgJ beta chain</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="7" type="primary">argJ</name>
        <name evidence="8" type="ORF">BN566_00538</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of N-acetylglutamate from glutamate and acetyl-CoA as the acetyl donor, and of ornithine by transacetylation between N(2)-acetylornithine and glutamate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>L-glutamate + N(2)-acetyl-L-ornithine = L-ornithine + N-acetyl-L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:15349"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:44337"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
            <dbReference type="EC" id="2.3.1.35"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>acetyl-CoA + L-glutamate = CoA + H(+) + N-acetyl-L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24292"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:44337"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis; L-arginine biosynthesis; L-ornithine and N-acetyl-L-glutamate from L-glutamate and N(2)-acetyl-L-ornithine (cyclic): step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 7">Heterotetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="7">Some bacteria possess a monofunctional ArgJ, i.e., capable of catalyzing only the fifth step of the arginine biosynthetic pathway.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the ArgJ family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.35" evidence="7"/>
    <dbReference type="EC" id="2.3.1.1" evidence="7"/>
    <dbReference type="EMBL" id="CBAR010000267">
        <property type="protein sequence ID" value="CCZ84685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00538"/>
    <dbReference type="EnsemblBacteria" id="CCZ84685">
        <property type="protein sequence ID" value="CCZ84685"/>
        <property type="gene ID" value="BN566_00538"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00106"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004042">
        <property type="term" value="F:acetyl-CoA:L-glutamate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004358">
        <property type="term" value="F:glutamate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103045">
        <property type="term" value="F:methione N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02152">
        <property type="entry name" value="OAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01106">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002813">
        <property type="entry name" value="Arg_biosynth_ArgJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016117">
        <property type="entry name" value="ArgJ-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042195">
        <property type="entry name" value="ArgJ_beta_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23100">
        <property type="entry name" value="PTHR23100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01960">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56266">
        <property type="entry name" value="SSF56266"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00120">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="5 7" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_5023273426" description="Arginine biosynthesis bifunctional protein ArgJ alpha chain" evidence="7">
        <location>
            <begin position="1"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023273427" description="Arginine biosynthesis bifunctional protein ArgJ beta chain" evidence="7">
        <location>
            <begin position="206"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="206"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="418"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="423"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole" evidence="7">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <feature type="site" description="Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole" evidence="7">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by autolysis" evidence="7">
        <location>
            <begin position="205"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ84685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="423" mass="44150" checksum="5DC3670059731150" modified="2013-07-24" version="1">MKAFEGYTYLDGGICAAKGFQASGTYCGIKKAMAPDSNEGEIGKEKNDIALVVADTLCNTAAVYTQNKVKGAPILVMKKHLAATGGKARALIANSKNANTCNADGEEKAEAMCKLTADALGIAPEEVMVMSTGVIGQILPLEPIEKAIPVLCEKLSPNGNLEAATAIMTTDTVSKEVAVSFTLDGKTCHLGGMAKGSGMIHPNMATTLNCITTDAAISAELLQTALSDVVKVTYNCLSVDGDQSTNDTCMVMASGLAGNAEITEQNADYAVFRQALYIVMMNLTRKLAKDGEGASKLLECTVSGAKDLDTAIIIAKSVICSPLFKCAMFGADANWGRVLCAVGYAPAEFDIHAVSVTLASRAGSVLVCEHGAGVAFSEEEAKKILLEDEIQILVSVGDGAGTATAWGCDLTYDYVKINGDYRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>R5V449</accession>
    <name>R5V449_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">dCMP deaminase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_00696</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytidine and deoxycytidylate deaminase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000022">
        <property type="protein sequence ID" value="CCZ82894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00696"/>
    <dbReference type="EnsemblBacteria" id="CCZ82894">
        <property type="protein sequence ID" value="CCZ82894"/>
        <property type="gene ID" value="BN566_00696"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004132">
        <property type="term" value="F:dCMP deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006220">
        <property type="term" value="P:pyrimidine nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01286">
        <property type="entry name" value="deoxycytidylate_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016192">
        <property type="entry name" value="APOBEC/CMP_deaminase_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016473">
        <property type="entry name" value="dCMP_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015517">
        <property type="entry name" value="dCMP_deaminase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035105">
        <property type="entry name" value="Deoxycytidylate_deaminase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11086">
        <property type="entry name" value="PTHR11086"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006019">
        <property type="entry name" value="dCMP_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="SSF53927"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00903">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="7">
        <location>
            <begin position="8"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="79"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR006019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR006019-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ82894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="159" mass="17858" checksum="4C1CC5B1D187A47E" modified="2013-07-24" version="1">MKRMDYLTWDAYFMGVALLSAQRSKDNHTQVGACIVNAENKVVSVGYNGMPTGCNDDVMPWERDGGFLDTKYAYVCHAELNAILNRNAASLQDCRLYVSLFPCNECAKAIIQSGIREVIYLDNKYADTESTKASVQMFRLAGVTCREYQPMQKDITLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5V484</accession>
    <name>R5V484_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative cellulosomal scaffoldin protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00729</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000029">
        <property type="protein sequence ID" value="CCZ82929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82929">
        <property type="protein sequence ID" value="CCZ82929"/>
        <property type="gene ID" value="BN566_00729"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004394827" evidence="1">
        <location>
            <begin position="23"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="520" mass="56115" checksum="3E635927476CAB2F" modified="2013-07-24" version="1">MKKIISLLAACTLFAVCLPVSAADAVTEDSALLQNSVVEQTDPYTFYFVPERTYVSAEEVQAGDVHIPTKVYIKGNTQPIKQITGARIRYQSDSSSLYFSNSSNILSTTDMYKQSYSGGSFFSEYAMPTCFGEITSSGTYTPYSCAPLTSALLTEPFSGSSIRSDGAGGIYFTRSYNKLVDGVKTWISNEKIEIPASDLQQNADGTVSFTYSYYKQSAEEQYPLETNTCTIPHYDATLPAGTVLSGYHDAYSWILTGTNAQEGVSFLGYADEFPFFSFDIVIRQGTPNDVYTVSFSETDCSLSTAAHTRLPLSFVSTSITVGASSATITNDTSKLSTICAFAENDRPLTLSQYSNAAITGRVTYTDNVTKNESLLDALTAQTTPAALYAAATTTGYAIADVPFYCGDATAPVYQFNAAPYTKKVLVGKKGDSNLDGIVDVNDSVCILTYYAKKAAGNTAKLNNSFTDDQETLAYFLSDIDTCSQNQGADGGVLGVEDAVQILTHYAKSASGNTPIWNNRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>R5V4M5</accession>
    <name>R5V4M5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Beta-mannanase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_00827</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 26 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000040">
        <property type="protein sequence ID" value="CCZ83034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00827"/>
    <dbReference type="EnsemblBacteria" id="CCZ83034">
        <property type="protein sequence ID" value="CCZ83034"/>
        <property type="gene ID" value="BN566_00827"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016985">
        <property type="term" value="F:mannan endo-1,4-beta-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006080">
        <property type="term" value="P:substituted mannan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005084">
        <property type="entry name" value="CMB_fam6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022790">
        <property type="entry name" value="GH26_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000805">
        <property type="entry name" value="Glyco_hydro_26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40079">
        <property type="entry name" value="PTHR40079"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16990">
        <property type="entry name" value="CBM_35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02156">
        <property type="entry name" value="Glyco_hydro_26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00739">
        <property type="entry name" value="GLHYDRLASE26"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51175">
        <property type="entry name" value="CBM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51764">
        <property type="entry name" value="GH26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004406718" evidence="5">
        <location>
            <begin position="26"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="domain" description="CBM6" evidence="6">
        <location>
            <begin position="79"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="GH26" evidence="7">
        <location>
            <begin position="219"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="382"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="556" mass="61516" checksum="F51E0A5D37623EAE" modified="2013-07-24" version="1">MKRIGSIILSMLCFFVMTGCQTVQQYPRVPQSVPLSTTETTEPPTESVPETMATTTIQGPSYPQETVSAPASMEMPAEVRVQAEDCKLSTLFTVENERSGYTGSGYVAGLSGDLQNTLLFQVSVPSSQHYDISVVMAADADATCLMTVEGTPYNLLTIPGTGKFVQATVQGVYLEAGTRTITIQQESGDALIDCILLKNAEKQDSVSISSELCDPNATEETKNLMQFLVEQYGKTMISGQYVSSAENTELTQIYQITGQLPLIRFADLYGYSGNGGSPEEADAIDSSLEWAERGGIVGLMWHWNAPIGTASVYKDETDFSLANAVTDADIAHLNRYELQQLRKDGTISAECIALIEDIDAAAEALKELQEAGVPVLWRPLQEASNGWFWWGASGSEAYQWLWHLMYTRMVDYHQLHNLIWIWNGQSAEYLVPDTEYDIAAADIYVDADEAYGSRYEQFFALQKIAKNKMVALSECSTIPDLTAIRRDQAIWSFFGLWYGEYLMDDAAGYTTKQQMIDAYNSDLVLTLSEYQAWYAPKQTTTGESTVQTETTTTTIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5V4N3</accession>
    <name>R5V4N3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00842</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000042">
        <property type="protein sequence ID" value="CCZ83049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83049">
        <property type="protein sequence ID" value="CCZ83049"/>
        <property type="gene ID" value="BN566_00842"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="96" mass="10811" checksum="63C99ED42D8C5AD4" modified="2013-07-24" version="1">MKALAYAFSAENPHALRNILLVAVTIAYLVVIVVLFLRLRHANQKMADRMKELSKQKQAEQAAKEAETAAQEEPLTSKLHTCRPRTEETHTDPKVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5V4N6</accession>
    <name>R5V4N6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">PHP domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00847</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000044">
        <property type="protein sequence ID" value="CCZ83054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00847"/>
    <dbReference type="EnsemblBacteria" id="CCZ83054">
        <property type="protein sequence ID" value="CCZ83054"/>
        <property type="gene ID" value="BN566_00847"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="SSF89550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="167" mass="18134" checksum="AC8471E7B12ED1C2" modified="2013-07-24" version="1">MAAANNGCTTGRPHFAKTMLLKGYVSSVSEAFDRYLGTPEFLEKVERPKPTAVESIHLIQAAGGIAVLAHPAKLNLSQPDFLQLLSTLCEAGLRGIEAYYSTHSPEEMAWYADIAAQHGLFCTCGSDFHGEIIKPDIALGTGRNHSLCLPDELECQILERFQAALSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5V4V4</accession>
    <name>R5V4V4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DNA repair protein RadC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_00926</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000062">
        <property type="protein sequence ID" value="CCZ83139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00926"/>
    <dbReference type="EnsemblBacteria" id="CCZ83139">
        <property type="protein sequence ID" value="CCZ83139"/>
        <property type="gene ID" value="BN566_00926"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08071">
        <property type="entry name" value="MPN_DUF2466"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025657">
        <property type="entry name" value="RadC_JAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001405">
        <property type="entry name" value="UPF0758"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30471">
        <property type="entry name" value="PTHR30471"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04002">
        <property type="entry name" value="RadC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MPN" evidence="6">
        <location>
            <begin position="23"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ83139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="150" mass="17125" checksum="98F691857DD72D8E" modified="2013-07-24" version="1">MLPELFRYYQLSKETEQENRKQLELDRVSFMATHLQHLYTGVQREQVAVLLLDNSLQIIQECFLSEGSVTISTLSIRSIAELAFKYQAACVILAHNHPGGSASPSQEDCYVTKQVYTGLQYLNVALEDHYIIGKNAVISMREHGFWETLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5V5P5</accession>
    <name>R5V5P5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Sodium ion-translocating decarboxylase beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_01223</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000105">
        <property type="protein sequence ID" value="CCZ83449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01223"/>
    <dbReference type="EnsemblBacteria" id="CCZ83449">
        <property type="protein sequence ID" value="CCZ83449"/>
        <property type="gene ID" value="BN566_01223"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005661">
        <property type="entry name" value="OadB_MmdB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35806">
        <property type="entry name" value="PTHR35806"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03977">
        <property type="entry name" value="OAD_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01109">
        <property type="entry name" value="Na_pump_decarbB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="13"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="155"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="178" mass="18483" checksum="9C4AFAEAF96A5FBF" modified="2013-07-24" version="1">MEQNRQVSKTEKIIFPIVVSLFVILLLPSTAPLIGCLMLGNLWRECGVTARLSDTVQNALMNIVTVFLGISVGATTVASSFLSLQTLYIVGLGLLAFCFSTIGGLLVGKLMYKLSGGKINPLIGSAGVSAVPMAARVSQIEGQKANPSNFLLMHAMGPNVAGVIGSAIAAGFLLAVFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5V5R0</accession>
    <name>R5V5R0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Terminase small subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01233</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000109">
        <property type="protein sequence ID" value="CCZ83459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83459">
        <property type="protein sequence ID" value="CCZ83459"/>
        <property type="gene ID" value="BN566_01233"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051276">
        <property type="term" value="P:chromosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005335">
        <property type="entry name" value="Terminase_ssu"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03592">
        <property type="entry name" value="Terminase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="165" mass="18340" checksum="FA2440F7E128122F" modified="2013-07-24" version="1">MIMARRTIRPQERCFCIWYAMLCNVQEAALHAGYPQEEALERGVLLLQRADCRELIQSVLKSLSLRSPIPQVLAGLERLAFGSCNDAAKLIFSADNLSPEELAKLDLFNVTEIKRDKNGGVEIKLCDRQKAMEQMLTYASRTETQSQADALLTALTGGTAHADNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5V636</accession>
    <name>R5V636_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized conserved protein contains S4-like domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01337</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000131">
        <property type="protein sequence ID" value="CCZ83569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01337"/>
    <dbReference type="EnsemblBacteria" id="CCZ83569">
        <property type="protein sequence ID" value="CCZ83569"/>
        <property type="gene ID" value="BN566_01337"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040591">
        <property type="entry name" value="YlmH_RBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17774">
        <property type="entry name" value="YlmH_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YlmH_RBD" evidence="1">
        <location>
            <begin position="88"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="253" mass="28621" checksum="5C2485444757A5A1" modified="2013-07-24" version="1">MNRRETESESVFFSRLQDLADRCRRDCAPDCTMFLDEMQCAAADAFLQRQAELAYQFWGGYEQAERKCCCLYPDFLEFDPAWVGCRCVTIRYSNLQTLEHRDFLGAALGCGLKRETIGDILIEKGKAQLWATDAAAALLVQSLEKVGRGGVRCTDTEPFSIVPVRKRIEHHGTVSSLRCDAIAAVVTRQSREKAVRIIQQGYMERSGHTVLQPSEHMQTGDIFVIRGSGKFQLISVSEPSKKGRLHIFIEQYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5V643</accession>
    <name>R5V643_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Methylenetetrahydrofolate reductase</fullName>
            <ecNumber evidence="10">1.5.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN566_01347</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2 10">
            <text>(6S)-5-methyl-5,6,7,8-tetrahydrofolate + NAD(+) = (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:19821"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:18608"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.5.1.20"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>(6S)-5-methyl-5,6,7,8-tetrahydrofolate + NADP(+) = (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:19817"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:18608"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.5.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3 10">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 10">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 10">Belongs to the methylenetetrahydrofolate reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.20" evidence="10"/>
    <dbReference type="EMBL" id="CBAR010000132">
        <property type="protein sequence ID" value="CCZ83579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01347"/>
    <dbReference type="EnsemblBacteria" id="CCZ83579">
        <property type="protein sequence ID" value="CCZ83579"/>
        <property type="gene ID" value="BN566_01347"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106312">
        <property type="term" value="F:methylenetetrahydrofolate reductase NADH activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106313">
        <property type="term" value="F:methylenetetrahydrofolate reductase NADPH activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00537">
        <property type="entry name" value="MTHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029041">
        <property type="entry name" value="FAD-linked_oxidoreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003171">
        <property type="entry name" value="Mehydrof_redctse-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004620">
        <property type="entry name" value="MTHF_reductase_bac"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02219">
        <property type="entry name" value="MTHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51730">
        <property type="entry name" value="SSF51730"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00676">
        <property type="entry name" value="fadh2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0274">FAD</keyword>
    <keyword evidence="6 10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="8 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ83579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="283" mass="31243" checksum="8F59CEBE56AE6691" modified="2013-07-24" version="1">MSSPNKTTFSFEIFPPKPTSEPSVIYGTLDALQDLKPDFISVTYGAGGGANCKNTVGIASYIQNTCQTESVAHLPCLYLSESDLNGILEQLQENGIHNILALRGDRSETATPNGRFSHANELISYIHDYDPSFHIMAACYPEGHPEQHSPAEELETLKRKVDAGASQFITQLFLDNDAFDTYRNKVAQAGVSVPIEAGIMPVTNKKQIERMVKLCGATLPKKFLHILNKYEDNPEALRDAGVAYAIEQIVDLIAQDVDGIHLYTMNRADVARDIDHAVHRLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5V6R3</accession>
    <name>R5V6R3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted Zn-dependent peptidases</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01544</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000164">
        <property type="protein sequence ID" value="CCZ83809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01544"/>
    <dbReference type="EnsemblBacteria" id="CCZ83809">
        <property type="protein sequence ID" value="CCZ83809"/>
        <property type="gene ID" value="BN566_01544"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="SSF63411"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_M16_C" evidence="1">
        <location>
            <begin position="71"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="310" mass="34625" checksum="E9650AF4DBDFCD79" modified="2013-07-24" version="1">MLLTCLLQLNCVADGFTEFEFQTKQKDLLDTIEAEINEKRAYATAQALETAFQNEPAALSCYGKKEDVLALTPQNTLAAYREVLHTAPAEIYFVGAESQPMLLEKLKAALTPIDRPNVPSYTFHVPSPAKTEPVTVVEPLPVNQAKMVLVLKSDCDNRYVMQMVSYILGALPSSKLFANVREKMSLCYYCSSSYSTPKQAMIISSGVEHDNLEKAKTAILEQLTAIQNGDISDAELESARMSIYNTLNGIGDTPGSHINWYQGCYYWNNYHTPEDSLKQYLAITKEDITKAARTLKLDTVYIMDTKKEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>R5V705</accession>
    <name>R5V705_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">C-terminal peptidase (Prc)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_01613</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the peptidase S41A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000169">
        <property type="protein sequence ID" value="CCZ83884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01613"/>
    <dbReference type="EnsemblBacteria" id="CCZ83884">
        <property type="protein sequence ID" value="CCZ83884"/>
        <property type="gene ID" value="BN566_01613"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07560">
        <property type="entry name" value="Peptidase_S41_CPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004447">
        <property type="entry name" value="Peptidase_S41A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005151">
        <property type="entry name" value="Tail-specific_protease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13180">
        <property type="entry name" value="PDZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03572">
        <property type="entry name" value="Peptidase_S41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00245">
        <property type="entry name" value="TSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="SSF50156"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00225">
        <property type="entry name" value="prc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="PDZ" evidence="4">
        <location>
            <begin position="97"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="395" mass="42848" checksum="174E4C50AA3A1662" modified="2013-07-24" version="1">MQKRTFFKRLFVGLLLAIIVCMGVVIYRLQASLHAVQTALGDNQRISVLEQAVGDNYYESVDDSKLMDGALKGYISALDDPYSGYLTNEEYHQWQSNEAGVSVGIGVTVTLTEDESGLQIVSVENPSPAKLAGLQADDIITAVDGETVADLGYQEAVSHVRGEADTSVELTIQRGKTELKKTVQRQEMQVTSAYGTMLENKIGYIRIASFKENTVEQFQNALNQLLKQGAKGLVFDVREDGGGLVAALEKIVDPLLPAGDIATATYQDGTTKVLVHSDANELNLPMMVLVNGNTASAAELFTASLKDFGKADVIGEQTFGKGIMQNTFELPDGGAVTLTVATYQTTKGECYHGVGITPDVIMEEDYEKVDFDHPNSEQDAQLKQAMELLQSKINS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7F8</accession>
    <name>R5V7F8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01733</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000178">
        <property type="protein sequence ID" value="CCZ84004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84004">
        <property type="protein sequence ID" value="CCZ84004"/>
        <property type="gene ID" value="BN566_01733"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025962">
        <property type="entry name" value="SdpI/YhfL"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13630">
        <property type="entry name" value="SdpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="128" mass="14774" checksum="BAB7048A36E6468D" modified="2013-07-24" version="1">MKVFDIVFMVLIPLVLLFAGWMMQFHPPKKQNVWIGYRTTRSMKNAETWRFANTYCGKCWCISGGILLLIAVGCSVGYFFMSMKMQTMVLLGNIVLQMAVLICTIFQTERAIRTQFDETGKRKDTTHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7J3</accession>
    <name>R5V7J3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative iron compound ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_01785</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000184">
        <property type="protein sequence ID" value="CCZ84059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01785"/>
    <dbReference type="EnsemblBacteria" id="CCZ84059">
        <property type="protein sequence ID" value="CCZ84059"/>
        <property type="gene ID" value="BN566_01785"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="6"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ84059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="369" mass="41045" checksum="247A28487FB09E94" modified="2013-07-24" version="1">MKPILLHTEQLTVGYRKKALIPDITFSVQAGQILTLIGANGVGKSTILKSIARQLEPIAGTIFVGEQELSAISHRNLAKSMSILMTHSFQTEFLRCIDVVEAGRYPYTGQLGILSEQDHEKVAEALRLVHGEELAQKDFRKISDGQRQRILLAKAICQEAEILILDEPTSFLDVRYKLKFLTILKDLVREKQIAVILSLHELDLAQRISDVVLCIRNHTVDRIGTPDEIFTDAYITNLYGITCGSYESLYGTMELPAPTGTPQTFVIGGGGTGNAVYRQLQRENIPFYAGILQENDLDYPVAKALATEVITEKAFEPIGEPTFQKASSLLQHCERVILCCKQFGTMNQKNQELILLAKENGKQILEANS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5V810</accession>
    <name>R5V810_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GtrA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_00193</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GtrA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000204">
        <property type="protein sequence ID" value="CCZ84264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00193"/>
    <dbReference type="EnsemblBacteria" id="CCZ84264">
        <property type="protein sequence ID" value="CCZ84264"/>
        <property type="gene ID" value="BN566_00193"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007267">
        <property type="entry name" value="GtrA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04138">
        <property type="entry name" value="GtrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="33"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="64"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="101"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="132"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="GtrA" evidence="7">
        <location>
            <begin position="35"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ84264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="163" mass="18512" checksum="0782A6CB3E62D44F" modified="2013-07-24" version="1">MKSEHLTEVKQILKQHQFKKLFSGDTENTFIQFFRYCFVGGFATVVDWALSSLLFYLAFSGSGYAALCNGLSFVAGLLVNYFLSTFWIFKQSKMQNRFLEFLSFAAIGVVGLLLTIGITKGFAWALADVTSLYQILSKVVSTAVAFFWNFFARKYLLYTKKEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>R5V854</accession>
    <name>R5V854_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alanine racemase</fullName>
            <ecNumber evidence="3">5.1.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_00227</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the interconversion of L-alanine and D-alanine. May also act on other amino acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>L-alanine = D-alanine</text>
            <dbReference type="Rhea" id="RHEA:20249"/>
            <dbReference type="ChEBI" id="CHEBI:57416"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="EC" id="5.1.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 3 4">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the alanine racemase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.1.1.1" evidence="3"/>
    <dbReference type="EMBL" id="CBAR010000212">
        <property type="protein sequence ID" value="CCZ84314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00227"/>
    <dbReference type="EnsemblBacteria" id="CCZ84314">
        <property type="protein sequence ID" value="CCZ84314"/>
        <property type="gene ID" value="BN566_00227"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00042">
        <property type="reaction ID" value="UER00497"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008784">
        <property type="term" value="F:alanine racemase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030632">
        <property type="term" value="P:D-alanine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01201">
        <property type="entry name" value="Ala_racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000821">
        <property type="entry name" value="Ala_racemase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009006">
        <property type="entry name" value="Ala_racemase/Decarboxylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011079">
        <property type="entry name" value="Ala_racemase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001608">
        <property type="entry name" value="Ala_racemase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020622">
        <property type="entry name" value="Ala_racemase_pyridoxalP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00842">
        <property type="entry name" value="Ala_racemase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01168">
        <property type="entry name" value="Ala_racemase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00992">
        <property type="entry name" value="ALARACEMASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01005">
        <property type="entry name" value="Ala_racemase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50621">
        <property type="entry name" value="SSF50621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="SSF51419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00492">
        <property type="entry name" value="alr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00395">
        <property type="entry name" value="ALANINE_RACEMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3 4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ala_racemase_C" evidence="6">
        <location>
            <begin position="252"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; specific for D-alanine" evidence="3">
        <location>
            <position position="41"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; specific for L-alanine" evidence="3">
        <location>
            <position position="273"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="321"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="3 4">
        <location>
            <position position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR600821-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR600821-52"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ84314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="386" mass="42522" checksum="3D92515C48DD14BA" modified="2013-07-24" version="1">MHQPSILHRAWAEIDLSALENNVRCIQEILPEHTDFMAVVKADAYGHGDAVICTALHKMGIRWFAVSNLEEALSVRQYCPDSEIFILGYTPPELAPELTKHHIIQGVMSTEYAQELAAHAGSEPVRCHIKLDTGMGRIGFRSADPQACVEELLPLFALPQLKIEGLYTHFAVADCPNDAENTAYTDEQERRILAVYDLLAENGHQLQHLHFMNSAATVYRPNPRCTLARVGIILYGLLPNYPVPVPLPLKPVMTLKSVISHIKEAEAGDCISYGRTYTVSHPCRIATVTIGYADGYSRLLSNKAYAIVHGVACPIVGRVCMDQLMLDISEVKDPVKPGDEVTMFGTEGNVTITPDELAAFYGTIGYEIVCGISKRVPRVVVNGDIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5V8G0</accession>
    <name>R5V8G0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Carbon-nitrogen hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00311</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000224">
        <property type="protein sequence ID" value="CCZ84404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00311"/>
    <dbReference type="EnsemblBacteria" id="CCZ84404">
        <property type="protein sequence ID" value="CCZ84404"/>
        <property type="gene ID" value="BN566_00311"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050126">
        <property type="term" value="F:N-carbamoylputrescine amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006596">
        <property type="term" value="P:polyamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017755">
        <property type="entry name" value="N-carbamoylputrescine_amidase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="SSF56317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03381">
        <property type="entry name" value="agmatine_aguB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="1">
        <location>
            <begin position="4"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="297" mass="33633" checksum="152D1C85B6C8396B" modified="2013-07-24" version="1">MRMVTVAAVQMHCSRDVTDNLERAEQLVRQAAAQGANVILLPELFERQYFCQERQYDYYQYATPLLENPAVQRFQKVAAELQVVLPISFYERAGNNTFNSVAVLDADGSVLGCYRKTHIPDDHFYQEKFYFTPGDTGFRVWNTRYGCIGVGICWDQWFPETARCMALQGAELLFYPTAIGSEPLLGMDSSGHWQRCMQGHAAANLMPVIAANRIGTETVEPCEANQNQQSELTFYGTSFITDQTGAVLKQAGRTEDTVLLHTFDLDAIQKDRLEWGLFRDRRPEQYGAISSPILVQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5V8K4</accession>
    <name>R5V8K4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cell division protein FtsI/penicillin-binding protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00043</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000230">
        <property type="protein sequence ID" value="CCZ84444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00043"/>
    <dbReference type="EnsemblBacteria" id="CCZ84444">
        <property type="protein sequence ID" value="CCZ84444"/>
        <property type="gene ID" value="BN566_00043"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008658">
        <property type="term" value="F:penicillin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001460">
        <property type="entry name" value="PCN-bd_Tpept"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00905">
        <property type="entry name" value="Transpeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transpeptidase" evidence="1">
        <location>
            <begin position="135"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="448" mass="49473" checksum="238F468E4962832F" modified="2013-07-24" version="1">MKSIKRGERLTILLIVLFLLGMVLLVARIVSQATFYMSHSHNVKLGYIYDRNGEVLFDEDATAEKYGADYFLDVGNLIGDNSGRMTNTLVSENLKDLQNYNLLFGAVQNGKSAIYTTLDHDANQQVYQSFGEKNGTAIAYNYQTGEILVCVSKPSVNILSDVDVSTLETGSLLCKAFYQTIPGSTQKVCTTAAAIETIGYDALMQKSYDCQGTYYNNYGSPIRCYHGSVHGWQNISQAFQHSCNPWFAQLVEDLPLDGLQTAFRKMGYQVNGEGSTQMDLDGISVFTASTTLEDTNDSITQWGCIGQGETVISPLQLMMWESAIANGTGQVTQPYLISYRQDLFGTVRDKAKATYSNEIFSAETAQQVRAIMRENGDLARYSTINYPVGLKTGTAEQGDDTDNSLLVGFCDDSNLPIAFCIEIEDWNQDVDTVNTNNIARTMLNALSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>R5V8K9</accession>
    <name>R5V8K9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA topoisomerase (ATP-hydrolyzing)</fullName>
            <ecNumber evidence="3">5.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_00048</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the type II topoisomerase GyrA/ParC subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="3"/>
    <dbReference type="EMBL" id="CBAR010000230">
        <property type="protein sequence ID" value="CCZ84449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00048"/>
    <dbReference type="EnsemblBacteria" id="CCZ84449">
        <property type="protein sequence ID" value="CCZ84449"/>
        <property type="gene ID" value="BN566_00048"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00187">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.268.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.199.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006691">
        <property type="entry name" value="GyrA/parC_rep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035516">
        <property type="entry name" value="Gyrase/topoIV_suA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013760">
        <property type="entry name" value="Topo_IIA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013758">
        <property type="entry name" value="Topo_IIA_A/C_ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013757">
        <property type="entry name" value="Topo_IIA_A_a_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002205">
        <property type="entry name" value="Topo_IIA_dom_A"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03989">
        <property type="entry name" value="DNA_gyraseA_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00521">
        <property type="entry name" value="DNA_topoisoIV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00434">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101904">
        <property type="entry name" value="SSF101904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56719">
        <property type="entry name" value="SSF56719"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="TOP4c" evidence="7">
        <location>
            <begin position="28"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ84449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="744" mass="84288" checksum="D255681BBDC669A2" modified="2013-07-24" version="1">MAKKRTTTPKIKPIPNAYIEKAGIVEEERITDTLEKNYMPYAMSVIISRALPEIDGFKPSHRKLLYTMYKMGLLNGGRTKSANVVGQTMRLNPHGDSAIYDTMVRLARGNESLLYPYVDSKGNFGKAYSRDMAYAASRYTEVKLEPICNELFRDIDKNTVDFVPNYDNTMMEPTLFPTTFPSVLVNANVGIAVSMASNVCPFNLTEVCDTTIALLKNPEADVTETLLGPDFPGGGFLLKDADEFRKICETGRGSVKVRAKWNYDKAGGCIEVTEIPYSTTIEAIMDKIVDGIKQGKLREISYVRDETDLSGLKIAIDLKRGADPEKVMQKLFRMTPLQDSYSCNFNILIHGQPRVMGVKEILTEWITFREECVRRRIVYDLQRKREKLHLLEGLERILLDIDEAIRIVRETKEESEVVPNLMQGFSMDEIQAEYVAEIKLRHLNREYILRRTQEMEQLRTDIDSLQQTLESPKKIRAVIAKELKETAKKYGQPRKTQFCEVDLQAESDPTMDIPDYPVHLFLSESGYFKKISPASLRMNAEQKLKEDDRIIAHWETTNRIELLFFTNQQQVYKTKASAFEDSKASLLGDYIPAKLGFDEGEFVVSVILTNDYSGMLLFVYENGKVAKVPLQSYATKTNRKKLANAYSDKSPLVTALPLPEEQNVLLRSSGNRALLFQTEELLPKTARNTQGVQVMTLKGKQVVLSAELLTAEQAEERKRYQAKTLPALGALAKDLPDVGQMTLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>R5V8V7</accession>
    <name>R5V8V7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">50S ribosomal protein L2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rplB</name>
        <name evidence="8" type="ORF">BN566_00060</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in peptide bond synthesis. Stimulates efficient translation and peptide-bond synthesis on native or reconstituted 70S ribosomes in vitro. Probably functions indirectly by altering the affinity of the ribosome for aminoacyl-tRNA, thus increasing their reactivity as acceptors for peptidyl transferase.</text>
    </comment>
    <comment type="function">
        <text evidence="5">One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial. Makes several contacts with the 16S rRNA in the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the 50S ribosomal subunit. Forms a bridge to the 30S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the universal ribosomal protein uL2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000241">
        <property type="protein sequence ID" value="CCZ84514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00060"/>
    <dbReference type="EnsemblBacteria" id="CCZ84514">
        <property type="protein sequence ID" value="CCZ84514"/>
        <property type="gene ID" value="BN566_00060"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01320_B">
        <property type="entry name" value="Ribosomal_L2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022666">
        <property type="entry name" value="Rbsml_prot_L2_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002171">
        <property type="entry name" value="Ribosomal_L2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005880">
        <property type="entry name" value="Ribosomal_L2_bac/org-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022669">
        <property type="entry name" value="Ribosomal_L2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022671">
        <property type="entry name" value="Ribosomal_L2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014726">
        <property type="entry name" value="Ribosomal_L2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691">
        <property type="entry name" value="PTHR13691"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00181">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03947">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002158">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01383">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01382">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01171">
        <property type="entry name" value="rplB_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00467">
        <property type="entry name" value="RIBOSOMAL_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Ribosomal_L2_C" evidence="7">
        <location>
            <begin position="124"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="208"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="256"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ84514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="277" mass="30265" checksum="24C63C73585942AA" modified="2013-07-24" version="1">MAIKSYKPTTPSRRGMTVTDYSGLSKVAPEKSLLEPLKKNAGRNSYGRITVRHRGGGNRRKYRVIDFKRTKDGMNANVLTLEYDPNRSAFIALVQYEDGEKRYILAPEGLKVGDVVRSGSDADIKPGCALTLADIPVGTFIHAIELYPGKGAQLVRSAGNTAQLMGKEGAFALIRLPSGEMRKVSILCRASIGQVSNVDHENVNYGKAGRMRNKGWRPTVRGSVMNPNDHPHGGGEGKSPVGRPGPVTPWGKPALGYKTRKKHKASDKYIVKRRNGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VGZ9</accession>
    <name>R5VGZ9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00651</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000012">
        <property type="protein sequence ID" value="CCZ82846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82846">
        <property type="protein sequence ID" value="CCZ82846"/>
        <property type="gene ID" value="BN566_00651"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ82846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="145" mass="16093" checksum="508DB3A5BF0E95F3" modified="2013-07-24" version="1">MGLFGALNKMSGYSADNMNKVFDEFLPVMDSKAICPIGISLKAKGLFSSKPIASGFATITDTSLFVIYTIIPIKGVAIYDFRNAKRLVIRNQIANQKVIDAKLLNLRTQEFEDVCLQTAPKINGFPQQETNLNQMLAILKPYLTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>R5VHS9</accession>
    <name>R5VHS9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Acetate kinase</fullName>
            <ecNumber evidence="6">2.7.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Acetokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ackA</name>
        <name evidence="8" type="ORF">BN566_00110</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>acetate + ATP = acetyl phosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:11352"/>
            <dbReference type="ChEBI" id="CHEBI:22191"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="6">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="6">Mg(2+). Can also accept Mn(2+).</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Metabolic intermediate biosynthesis; acetyl-CoA biosynthesis; acetyl-CoA from acetate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the acetokinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.1" evidence="6"/>
    <dbReference type="EMBL" id="CBAR010000046">
        <property type="protein sequence ID" value="CCZ83056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00110"/>
    <dbReference type="EnsemblBacteria" id="CCZ83056">
        <property type="protein sequence ID" value="CCZ83056"/>
        <property type="gene ID" value="BN566_00110"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00340">
        <property type="reaction ID" value="UER00458"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008776">
        <property type="term" value="F:acetate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006085">
        <property type="term" value="P:acetyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00020">
        <property type="entry name" value="Acetate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004372">
        <property type="entry name" value="Ac/propionate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000890">
        <property type="entry name" value="Aliphatic_acid_kin_short-chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023865">
        <property type="entry name" value="Aliphatic_acid_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21060">
        <property type="entry name" value="PTHR21060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00871">
        <property type="entry name" value="Acetate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000722">
        <property type="entry name" value="Acetate_prop_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00471">
        <property type="entry name" value="ACETATEKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00016">
        <property type="entry name" value="ackA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01075">
        <property type="entry name" value="ACETATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01076">
        <property type="entry name" value="ACETATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6 7" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Proton donor/acceptor" evidence="6">
        <location>
            <position position="161"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="221"/>
            <end position="225"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="296"/>
            <end position="298"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="344"/>
            <end position="348"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="397"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="6">
        <location>
            <position position="193"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="6">
        <location>
            <position position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="414" mass="45325" checksum="470A3E2A50B0FA4A" modified="2013-07-24" version="1">MLQHRHHQKGLDFFMIILVVNAGSSSLKYQLIDMKDESVIAKGNCDRIGIDGHLSHKTADGRKVEEDCNFPTHAEAFERLVQALTTGDAAVISSMDEVSAVGHRVVQGAELFTKTTLVTDEVIDEIENLKELAPVHNHGHALALWACKKTMPNIPQVVVFDTAFHQTIPQKAFMYGFPYEDYEQYHVRKYGFHGTSHRFVTAKLAEVMGKDLKDLKVVSCHLGNGSSITAVKGGESIDTTMGFTPLDGVLMGTRCGAVDPSAVTYVAGKHGFHVADMDNYMNKKSGMLGISGLSSDFRDITAASEKGDARATLAKDMLVYDIKKDIGAFAAIMNGLDAVIFTGGIGENAPNCREEVCENLEYLGIKIDKEVNNFKGELKKISTPDSKVEVWVIPTNEELLIARDTLAMVEGLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5VHW5</accession>
    <name>R5VHW5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00908</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000060">
        <property type="protein sequence ID" value="CCZ83121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00908"/>
    <dbReference type="EnsemblBacteria" id="CCZ83121">
        <property type="protein sequence ID" value="CCZ83121"/>
        <property type="gene ID" value="BN566_00908"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045678">
        <property type="entry name" value="DUF6198"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038750">
        <property type="entry name" value="YczE/YyaS-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40078">
        <property type="entry name" value="PTHR40078"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19700">
        <property type="entry name" value="DUF6198"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="188"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="220" mass="24211" checksum="A185F03FCB8382AC" modified="2013-07-24" version="1">METIEKTRSFPNRLICYFVGLFIMTAGIAISVKSNLGVSPVSSIPYTITRCWGLEMGKATILFHCVLVLIQVLILRKRFQLKSLLQIPVGIVFGYFTTFCNYLMTFAPTVHNFALRIGMCLVSIVLVAIGIFFYMPANIMPLAGEGVMQAVSEVTKIAFPKVKVAFDLTMVVISLTTCLLVLHQFGSVGIGTILAAVLVGVVLGQIMRIWATIQKRKLAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>R5VI07</accession>
    <name>R5VI07_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Lysine--tRNA ligase</fullName>
            <ecNumber evidence="7">6.1.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Lysyl-tRNA synthetase</fullName>
            <shortName evidence="7">LysRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">lysS</name>
        <name evidence="11" type="ORF">BN566_00969</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7 8">
            <text>ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)</text>
            <dbReference type="Rhea" id="RHEA:20792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9696"/>
            <dbReference type="Rhea" id="RHEA-COMP:9697"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78529"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="7 8">Binds 3 Mg(2+) ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.6" evidence="7"/>
    <dbReference type="EMBL" id="CBAR010000069">
        <property type="protein sequence ID" value="CCZ83186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00969"/>
    <dbReference type="EnsemblBacteria" id="CCZ83186">
        <property type="protein sequence ID" value="CCZ83186"/>
        <property type="gene ID" value="BN566_00969"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004824">
        <property type="term" value="F:lysine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006430">
        <property type="term" value="P:lysyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00775">
        <property type="entry name" value="LysRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04322">
        <property type="entry name" value="LysRS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00252">
        <property type="entry name" value="Lys_tRNA_synth_class2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002313">
        <property type="entry name" value="Lys-tRNA-ligase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044136">
        <property type="entry name" value="Lys-tRNA-ligase_II_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018149">
        <property type="entry name" value="Lys-tRNA-synth_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00982">
        <property type="entry name" value="TRNASYNTHLYS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00499">
        <property type="entry name" value="lysS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 7 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II" evidence="10">
        <location>
            <begin position="205"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="523"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="437"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ83186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="546" mass="62130" checksum="87C61B17A81E9A63" modified="2013-07-24" version="1">MAQQQNPQQNAAAETEQDINILKKIRMDKLAELQAAGNDPFTITKYDVTAHAADLKADYEATEARLKEAANGDEEAFQASLEPLKEQIVSIAGRIMSWRDMGRANFIDVRDGSDRIQVYVRMNDIGGKEAFKEFKKWDIGDIVGVKGFVFRTKKGEISIHAQEITLLSKALLQLPEKWHGLKDQDTRYRKRYLDLIVNPEVKNTFVIRSKILREIRNYLDNIGYVEVETPVLLPLQIAAAARPFVTHHNTLDMDMFLRIETELNLKKLIVGGFERVYEVGRIFRNEGMDPSHNPEFTSIEMYQAYTDYKGMMDLIEDMYRTLAKTICGSDHILYQGVEIALGEPWERLTMVEAVKKYAGVDYYEWDSDEAARACAKEKGVEVEEGEHATKGHVLIAFFDAFVEENLIQPTIIYDYPVENSPLAKRKPSEPAFTERFEYFIYAREMGNAFSELNDPIDQKQRFEAQVAARRELGDMTGEVDEDFVNALEYGLPPTGGLGLGLDRLVMLLTDSASIRDVLLFPTMRPLPKNGQESEEDADEAEETTEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>R5VI48</accession>
    <name>R5VI48_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable tRNA sulfurtransferase</fullName>
            <ecNumber evidence="6">2.8.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Sulfur carrier protein ThiS sulfurtransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Thiamine biosynthesis protein ThiI</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">tRNA 4-thiouridine synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">thiI</name>
        <name evidence="9" type="ORF">BN566_01012</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + a uridine in tRNA + ATP + H(+) + 2 reduced [2Fe-2S]-[ferredoxin] = [ThiI sulfur-carrier protein]-L-cysteine + a 4-thiouridine in tRNA + AMP + diphosphate + 2 oxidized [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:24176"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:13337"/>
            <dbReference type="Rhea" id="RHEA-COMP:13338"/>
            <dbReference type="Rhea" id="RHEA-COMP:13339"/>
            <dbReference type="Rhea" id="RHEA-COMP:13340"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:61963"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:136798"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.8.1.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[sulfur-carrier protein ThiS]-C-terminal Gly-Gly-AMP + AH2 + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] = [sulfur-carrier protein ThiS]-C-terminal Gly-NH-CH2-C(O)SH + A + AMP + H(+) + L-cysteinyl-[cysteine desulfurase]</text>
            <dbReference type="Rhea" id="RHEA:43340"/>
            <dbReference type="Rhea" id="RHEA-COMP:12157"/>
            <dbReference type="Rhea" id="RHEA-COMP:12158"/>
            <dbReference type="Rhea" id="RHEA-COMP:12908"/>
            <dbReference type="Rhea" id="RHEA-COMP:12910"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:61963"/>
            <dbReference type="ChEBI" id="CHEBI:90618"/>
            <dbReference type="ChEBI" id="CHEBI:90619"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; thiamine diphosphate biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ThiI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.4" evidence="6"/>
    <dbReference type="EMBL" id="CBAR010000080">
        <property type="protein sequence ID" value="CCZ83231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01012"/>
    <dbReference type="EnsemblBacteria" id="CCZ83231">
        <property type="protein sequence ID" value="CCZ83231"/>
        <property type="gene ID" value="BN566_01012"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00060"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004810">
        <property type="term" value="F:tRNA adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140741">
        <property type="term" value="F:tRNA U4 sulfurtransferase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034227">
        <property type="term" value="P:tRNA thio-modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01712">
        <property type="entry name" value="ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00021">
        <property type="entry name" value="ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020536">
        <property type="entry name" value="ThiI_AANH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003720">
        <property type="entry name" value="tRNA_STrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02568">
        <property type="entry name" value="ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00981">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00342">
        <property type="entry name" value="TIGR00342"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="2 6 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="THUMP" evidence="8">
        <location>
            <begin position="62"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="185"/>
            <end position="186"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="210"/>
            <end position="211"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ83231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="399" mass="44312" checksum="0560FFC200C28874" modified="2013-07-24" version="1">MQTEIILAKYGELALKGLNRNTFEDILMKNIKRRLKPLGKFTYTRAQSTIYIEPETPDIDLDVVMDNMKKIFGIAALCKACVCEKDLADISKVGIPYLEEILLGVKTFKVEAKRADKQFPLKSPEICRELGGQILEAYPHLQVDVKHPDVTVMVEIREKHAFVHAGNEKGAGGLPVGSSGKALLLLSGGIDSPVAGYRMSRRGVHISAIHYVSPPYTSDRAQEKVEQLCQKLTAYCGSIAFFCVPFTEIQEEIKKNCPEPYFTIIMRRLMVEIAQKIAAKDDCSALITGESIGQVASQTMQGLVCTDAVSQIPIFRPLIGMDKTEIVETARQIDTFELSTLPYEDCCTVFTPKHPKVRPSLEEVEAAQAHYDFTASVEKAVANTEMKIFKLQNGTPFSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>R5VI98</accession>
    <name>R5VI98_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sulfate ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_01067</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01067"/>
    <dbReference type="EnsemblBacteria" id="CCZ83286">
        <property type="protein sequence ID" value="CCZ83286"/>
        <property type="gene ID" value="BN566_01067"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015419">
        <property type="term" value="F:ABC-type sulfate transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005666">
        <property type="entry name" value="Sulph_transpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013611">
        <property type="entry name" value="Transp-assoc_OB_typ2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08402">
        <property type="entry name" value="TOBE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="SSF50331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00968">
        <property type="entry name" value="3a0106s01"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0764">Sulfate transport</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="3"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="35"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ83286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="352" mass="39769" checksum="28AA972AA19A1443" modified="2013-07-24" version="1">MYVELKDINKHFNNYKASDHVSFGIEKGKLIGLLGPSGSGKTTILRMIAGLEQPDSGEIIIDGKVVNDVPASKRGIGFVFQNYALFRYMTVYDNIAFGLKVQKVDKKTIDKRVKELIELIGLKGLDKRYPSQLSGGQRQRVAFARALAPNPQLLLLDEPFAAIDAKVRQELRSWLKEMIEQLGVTSIFVTHDQDEAIEVADEIIITNKGRIEQMGTPLDVYQKPQTAFTASFFGQTSIIEDYQAFHTFEKIEGAEKAIVRPEFVKITKKNEVQKYRSSASKGVVEHVSFRGDKMELRVRVNDTVLLAKRDLEENRIFVGETVDVFLYRIFVTVGDKAYLLENQSLQENSVVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5VIU2</accession>
    <name>R5VIU2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Farnesyl-diphosphate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01215</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000105">
        <property type="protein sequence ID" value="CCZ83441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01215"/>
    <dbReference type="EnsemblBacteria" id="CCZ83441">
        <property type="protein sequence ID" value="CCZ83441"/>
        <property type="gene ID" value="BN566_01215"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="SSF48576"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="300" mass="32484" checksum="9217A64B88B661A8" modified="2013-07-24" version="1">MMTESEMKQLLQQDGKKVEAALQELLPDDAISALQRTSVVEAMRYSLEAGGKRIRPVLVLEFCKMFGGTEEQAMPAAAAIEMIHTFSLIHDDLPAMDDDDMRRGRPSCHKAFPEALAILAGDALSIHAFSVLANSTALSDAQKVRLITELAEESGHHGMIAGQVLDMEQEGRRDVTVDQLKTMCAFKTGALIRVACRMGCIAANATDEQIKAADTYGSYLGLAFQIVDDILDVTSTTDVLGKPVGSDAEENKVTFVTLLGLEAAQKEAAALTEKAMAMLKTLPNPEFLMALTQSLLSRKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJ43</accession>
    <name>R5VJ43_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative ABC-type transport system involved in lysophospholipase L1 biosynthesis permease component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_01270</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ABC-4 integral membrane protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000118">
        <property type="protein sequence ID" value="CCZ83501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01270"/>
    <dbReference type="EnsemblBacteria" id="CCZ83501">
        <property type="protein sequence ID" value="CCZ83501"/>
        <property type="gene ID" value="BN566_01270"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027022">
        <property type="entry name" value="ABC_permease_BceB-typ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018968">
        <property type="entry name" value="ABC_permease_BceB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="144"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="168"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="229"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="470"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="531"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="556"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX" evidence="7">
        <location>
            <begin position="30"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR018968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="600" mass="67257" checksum="3CA807CAA0D62D7C" modified="2013-07-24" version="1">MILSWGTGVVAIFAAIFLFYMNSFLMRRRKTEFGLYNILGMGKWNIARILCWQNGMLFGTSMVGGLGLGILLSKLSELMAAKMLGNSSPMGFRIEPNAVRNTILLTAVSYGIILLYSLGQIHVSKPIALLRGEKQGEKPPKARWLLTFCGLVMLGIAYYLSVTIQQPIAAFSMFFLAVVLVILATYLLFICGSVALCKLLQKNKRYYYQLQHFISVSSMRYRMKRNGSSLASICVLSTMVLVMISSTLCLYLGSEDSLYQRYPRDIFVTAYSDFDEQALEEKIAEVVSRYSLQEKNEIHETTLQISGVLNGNRLQFEQDSLVDYDDIATVKIFTLEEYNRLAGTSETLQDGEVLLSVIDGSYSGDTIQIGTMQPFQIQKQVPQFVDDGDAFANVSRMLYLIVPDMAMMQELNERQAEAYNGYASVIQFYKGFDLDADADTQLAIQAELEQQLSEEGCVCESRESGRSSFYALYGGLLFLGILLSVVFLFGTVLIMYYKQISEGYEDAGRFEILQKVGMTKKEIKRSINSQILTVFTAPLLMAGVHLAFAFPMLYRLLAVMGLINISLFAIITVGGFLVFAALYALMYKLTSHSYYQLVKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJB9</accession>
    <name>R5VJB9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">D-isomer specific 2-hydroxyacid dehydrogenase NAD binding domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00143</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000146">
        <property type="protein sequence ID" value="CCZ83616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00143"/>
    <dbReference type="EnsemblBacteria" id="CCZ83616">
        <property type="protein sequence ID" value="CCZ83616"/>
        <property type="gene ID" value="BN566_00143"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031629">
        <property type="entry name" value="DpaA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16924">
        <property type="entry name" value="DpaA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DpaA_N" evidence="2">
        <location>
            <begin position="6"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="2-Hacid_dh_C" evidence="1">
        <location>
            <begin position="135"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="284" mass="30699" checksum="D9E05BC76BB69408" modified="2013-07-24" version="1">MQQLLQILVAGGDRRQQYAAQKLAKSPQLSVTTIGFSQSDSETLPEFDVLILPIHTTAESVPSKNGNLSLPELLSHVKKGGLVCGGVLPEAVCRMCQEQELCFCDYFQREELCLANAVPTVEGAIQIAISERETVLCGSSVLVIGYGRIGTIMAERLQGLKADVTVAARSCKDAERCAANGLKWMHPSEIAARAGKFDWFCNTVPVLMLDETALFNMKPDALVIDLASKPGGTDFDAAKRLHRHVVWALGLPGITAPKTAGAIIAKTIFHILQERGLADEGNNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJE7</accession>
    <name>R5VJE7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA binding domain excisionase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01387</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000147">
        <property type="protein sequence ID" value="CCZ83651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83651">
        <property type="protein sequence ID" value="CCZ83651"/>
        <property type="gene ID" value="BN566_01387"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041657">
        <property type="entry name" value="HTH_17"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12728">
        <property type="entry name" value="HTH_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH_17" evidence="1">
        <location>
            <begin position="25"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="90" mass="10292" checksum="C6F9F3D20709E908" modified="2013-07-24" version="1">MNNIIREDNITPKTTLVLQPMPEQLLTVKDVSSLLKCNVATVHNLRKTGLLRFLKLGSYKCRVSTFLAFLDEYDGKDLDSLVQERQNEPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJV7</accession>
    <name>R5VJV7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA polymerase I</fullName>
            <ecNumber evidence="7">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">polA</name>
        <name evidence="10" type="ORF">BN566_01561</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">In addition to polymerase activity, this DNA polymerase exhibits 5'-3' exonuclease activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Single-chain monomer with multiple functions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the DNA polymerase type-A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="7"/>
    <dbReference type="EMBL" id="CBAR010000165">
        <property type="protein sequence ID" value="CCZ83826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01561"/>
    <dbReference type="EnsemblBacteria" id="CCZ83826">
        <property type="protein sequence ID" value="CCZ83826"/>
        <property type="gene ID" value="BN566_01561"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09898">
        <property type="entry name" value="H3TH_53EXO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020046">
        <property type="entry name" value="5-3_exonucl_a-hlix_arch_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002421">
        <property type="entry name" value="5-3_exonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019760">
        <property type="entry name" value="DNA-dir_DNA_pol_A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001098">
        <property type="entry name" value="DNA-dir_DNA_pol_A_palm_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020045">
        <property type="entry name" value="DNA_polI_H3TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018320">
        <property type="entry name" value="DNA_polymerase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002298">
        <property type="entry name" value="DNA_polymerase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10133">
        <property type="entry name" value="PTHR10133"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01367">
        <property type="entry name" value="5_3_exonuc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02739">
        <property type="entry name" value="5_3_exonuc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00476">
        <property type="entry name" value="DNA_pol_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00868">
        <property type="entry name" value="DNAPOLI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00475">
        <property type="entry name" value="53EXOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00482">
        <property type="entry name" value="POLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="SSF47807"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="SSF56672"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="SSF88723"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00593">
        <property type="entry name" value="pola"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00447">
        <property type="entry name" value="DNA_POLYMERASE_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 7" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="3 7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="53EXOc" evidence="8">
        <location>
            <begin position="1"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="POLAc" evidence="9">
        <location>
            <begin position="607"/>
            <end position="813"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ83826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="850" mass="95221" checksum="4F2C298ECEBE3F64" modified="2013-07-24" version="1">MRLLAIDGNSIMNRAFYGIRLLSNHKGIYTNAIFGFMNIYLKHVTALQPDAIAVAFDVHTPTFRHQKVSSYKANRKGMPEELAMQMPYLKKLLTLLGVQIVECPGYEADDVLGTLAQAMEEQGNDCTILSGDRDTLQLITDHVTVRLVTNREDIDYTVQRFREEYGFYPISMIDLKALMGDASDNISGVSGIGPKTASTLIRSWGTVENVYDHIHQLNVSKMAIQKLIAGQESAMESKWLATIVKDAPVPTELQHYLPKPIHAREVRDLLMELEMVKLLDKLHLEAAADQLEEEPLSCAITPSIAIQQQPLTDTLFQQWKTDLPTLCYELNLPEHRLQVLQEQTIYETTDEALIVAFLGSVIPKRTFGAKPHYRYMMQQGTALNNLVADGEIAAYLLNPASSEYTVARLSVTYHVLYDTALEDLAALVALPALCDAMEQEIQEQNMTDLYQMELELTRVLAEMEQAGVLVDVDGLETFGKYLSEQMEELQQMVYDEAGHSFKIGSPKQLGDVLFGEQKLPHGKKTKTGYSTNAEILESLREEYPIVDHVLKWRQVSKLKSTYIDGLKKTIAADGRIHTCFKQTETRTGRISSIEPNMQNIPVRTELGSNMRKFFVAGAGNVLLDADYSQIELRILANLCGDTAMQEAFLHGADIHTATAAQVFDLPPEMVTDSMRSAAKAVNFGIIYGIGAFSLSKDIHVSVAQADQYIKNYLARYPRVAAFMEETVEHAKELGYVSTYFKRRRPVPELRQSNKMIQASGKRIAMNTPIQGTAADIIKIAMIHVSNRLQEECPAAKLILQVHDELIVEVPEQQADLAAQILKEEMEHVVQFAVPLTVEVKKGKSWYDAKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>R5VKM7</accession>
    <name>R5VKM7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative dihydroxyacetone kinase regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01782</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000184">
        <property type="protein sequence ID" value="CCZ84056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84056">
        <property type="protein sequence ID" value="CCZ84056"/>
        <property type="gene ID" value="BN566_01782"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039532">
        <property type="entry name" value="TetR_C_Firmicutes"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14278">
        <property type="entry name" value="TetR_C_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="3"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="184" mass="21808" checksum="64F5033117EB2415" modified="2013-07-24" version="1">MSNITKRALEQSLKNLLLKKPLTKITISDITADCGMNRMTFYYHFKDIYDLVEWVCLEDAKRALDQKKTYDTWQQGLLQIFEAVQENKPFILNVYRCVHREQVEKYLQPLVDQLLLDVIQEESVGMTIREEDTQFIAKVYSYIFIGIMLDWIKNDMREEPKELVDRLAKLLYGSITAAIERFRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>R5VKS0</accession>
    <name>R5VKS0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Beta-mannanase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN566_01842</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 26 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000187">
        <property type="protein sequence ID" value="CCZ84116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01842"/>
    <dbReference type="EnsemblBacteria" id="CCZ84116">
        <property type="protein sequence ID" value="CCZ84116"/>
        <property type="gene ID" value="BN566_01842"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016985">
        <property type="term" value="F:mannan endo-1,4-beta-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006080">
        <property type="term" value="P:substituted mannan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005084">
        <property type="entry name" value="CMB_fam6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022790">
        <property type="entry name" value="GH26_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000805">
        <property type="entry name" value="Glyco_hydro_26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40079">
        <property type="entry name" value="PTHR40079"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16990">
        <property type="entry name" value="CBM_35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02156">
        <property type="entry name" value="Glyco_hydro_26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00739">
        <property type="entry name" value="GLHYDRLASE26"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51175">
        <property type="entry name" value="CBM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51764">
        <property type="entry name" value="GH26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004395220" evidence="6">
        <location>
            <begin position="33"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="domain" description="CBM6" evidence="7">
        <location>
            <begin position="36"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="GH26" evidence="8">
        <location>
            <begin position="174"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="9">
        <location>
            <begin position="743"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="684"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="686"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="335"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ84116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="820" mass="90283" checksum="DA13D0182F120FDC" modified="2013-07-24" version="1">MKIKKKVFAASLAAAMALNAVCVSSFFGGVSAAGETKYEFEDGKITGVSAEVEEVASASGGKIVHMRDAGETIKVTVNVEKAGMYDLNICYATDGGAKTQKLNVNGNAAGDLALSNHTDFSESNVARIKLNAGENELEFVSFWGWTQFDYLTLTEPNYPELSATSDLADKNATPETQSLMNYLASVYGEHIISGQQEIYKYGPHDFDYEFNYIKDNTGKLPAIRGFDFLNTNPLYGSEDGTVDRMISWVKDSKGIVTASWHITVPKKFSDYNIGDKVDWANGTYKPDETDFDTAKVLEEGTKEREYYMLCLKMLAEQIQKLQDAGVPMIFRPLHEAEGGGGEDGSWFWWGKSGSAVYKDLWKLTYKTLTEDYGLHNIIWEWNSYTFETSTNWYPGDEYVDLVAYDKYNCTDWSTGNAVLVHNDSAIGGTFYSLVEQYGGKKMVAMAENDSIPTLENLQMEKAGWLYFCPWYDGGSDSTNFLSNEMFNKKEDLKEIYTSDYCITLDELPENLYSNSGAVITTTSGTGQTTTTTTTTTTTTTMTTTKIENAIMGDIKKDGSTVKVIFDRSMGKEVHLIVDLDDSLGYANGCVGVSVKVDGVDYWVSYKWEASKSDDVTVSLDADNVYNVTFNNGSGTVKDADQIAKIAEAAQKEKEAQFQVWWANDKEGEKDDTTKATLVAAYLPDESGETTETTVTTTDAGTTTTPEETTTEPVESTTTSAEVTTAPNETTTTVTTAATTGSESEALYGDINMDGKVDLTDAVMLNKYQAGLVTLTDVQKVNANCDITDGTENITEEDSFALMRFILMMEDYENLPYNAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>R5VL53</accession>
    <name>R5VL53_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 15">Inosine-5'-monophosphate dehydrogenase</fullName>
            <shortName evidence="8">IMP dehydrogenase</shortName>
            <shortName evidence="8">IMPD</shortName>
            <shortName evidence="8">IMPDH</shortName>
            <ecNumber evidence="8 15">1.1.1.205</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">guaB</name>
        <name evidence="17" type="ORF">BN566_00205</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 15">
            <text>H2O + IMP + NAD(+) = H(+) + NADH + XMP</text>
            <dbReference type="Rhea" id="RHEA:11708"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="1.1.1.205"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="8">Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8 15">Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 14">Belongs to the IMPDH/GMPR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.205" evidence="8 15"/>
    <dbReference type="EMBL" id="CBAR010000206">
        <property type="protein sequence ID" value="CCZ84276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00205"/>
    <dbReference type="EnsemblBacteria" id="CCZ84276">
        <property type="protein sequence ID" value="CCZ84276"/>
        <property type="gene ID" value="BN566_00205"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00601">
        <property type="reaction ID" value="UER00295"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003938">
        <property type="term" value="F:IMP dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006177">
        <property type="term" value="P:GMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00381">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01964">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005990">
        <property type="entry name" value="IMP_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015875">
        <property type="entry name" value="IMP_DH/GMP_Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001093">
        <property type="entry name" value="IMP_DH_GMPRt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11911">
        <property type="entry name" value="PTHR11911"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00478">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000130">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01302">
        <property type="entry name" value="IMP_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00487">
        <property type="entry name" value="IMP_DH_GMP_RED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0129">CBS domain</keyword>
    <keyword evidence="3 8 15" id="KW-0332">GMP biosynthesis</keyword>
    <keyword evidence="2 8 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11 15" id="KW-0520">NAD</keyword>
    <keyword evidence="6 8 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5 8 12" id="KW-0630">Potassium</keyword>
    <keyword evidence="4 8 15" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="16">
        <location>
            <begin position="96"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="16">
        <location>
            <begin position="156"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="IMP binding" evidence="8 10">
        <location>
            <begin position="340"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="IMP binding" evidence="8 10">
        <location>
            <begin position="363"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="IMP binding" evidence="8 10">
        <location>
            <begin position="387"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="active site" description="Thioimidate intermediate" evidence="8 9">
        <location>
            <position position="307"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8 9">
        <location>
            <position position="403"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="250"/>
            <end position="252"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 11">
        <location>
            <begin position="300"/>
            <end position="302"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 12">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 12">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="305"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 12">
        <location>
            <position position="307"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="415"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="469"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="470"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="471"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CCZ84276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="488" mass="51838" checksum="D8BD4F4BB5F9B7F2" modified="2013-07-24" version="1">MEMCEKFGKQGLTFDDVLLIPAESDVTPNMIDLKTTLAGNVKLNTPIMTAAMDTVTEAAMAIAIAREGGIGIIHKNMSIEQQADEVDKVKRSENGVIVNPFSLTEDHLVADADKLMGKYKISGVPIVDRTGKLVGIITNRDMRFLTDYSAPISEVMTKDNLITAPVGTSMEKAQEILRQHKIEKLPLIDENGYLKGLITIKDIEKAVQFPNSARDEKGRLLCGAAIGITANVLERAHALVDAQVDVLVLDSAHGHSKNIMECLKKVKVAFPNTPVIAGNIATAEAAEALIQAGADAVKVGIGPGSICTTRIVAGIGVPQVTAVYDVACVAKKYGIPVIADGGIKYSGDIVKALAAGANVVMLGSLLAGCEEAPGETEIYQGRSFKVYRGMGSLAAMANGSKDRYFQEGAKKLVPEGVEGRVPYKGSVADSIFQLVGGIRSGMGYCGCQTIPLLHEKAKFVQITGAGLKESHPHDIQITKEAPNYSAHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VLE1</accession>
    <name>R5VLE1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00273</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000220">
        <property type="protein sequence ID" value="CCZ84366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84366">
        <property type="protein sequence ID" value="CCZ84366"/>
        <property type="gene ID" value="BN566_00273"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="254" mass="28787" checksum="F81C6DD2317C8319" modified="2013-07-24" version="1">MGLDIYAGTLTRYYTQNWKTAVQQFGEAHGIPVQIIRANPEEHPADEAEVLERVTEWRKHILNGLGITEPPLWNEDANQTPYYTDKPDWDGITALQLFVLAKMFGEPVPETISKNADLTEFPLYQARQAYLKQHNEPVSILEGCGWWVPLDEPAIFSYVLPNFEEGLLATVGTLKRELNAINALSWNATSEEIASWRNTEGYPYDGTVQMNGDISIQKNHTVYDTVSLAKFAFSILWEAADFAMQHGVVVLYDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5VLF1</accession>
    <name>R5VLF1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Nodulation efficiency protein D</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00283</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000222">
        <property type="protein sequence ID" value="CCZ84376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00283"/>
    <dbReference type="EnsemblBacteria" id="CCZ84376">
        <property type="protein sequence ID" value="CCZ84376"/>
        <property type="gene ID" value="BN566_00283"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002810">
        <property type="entry name" value="NfeD-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01957">
        <property type="entry name" value="NfeD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="26"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="NfeD" evidence="3">
        <location>
            <begin position="51"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="162" mass="17462" checksum="AB0DD317F7A92DCB" modified="2013-07-24" version="1">MVFWIWGSLFVLTVIAEIASQQLISIWFAAGSLVALLAALCGAPIWLQALLFIAASALLLILTRPLVRKFTNFASRDTNLRLDLGKFGTVVQTIDAEKGTGRVRVDDIDWIAVSQPNLVIPKGTTVQVQAIDGTKLFVTPVPQTTEPVVESANATSSSEHTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5VLJ0</accession>
    <name>R5VLJ0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Imidazolonepropionase and related amidohydrolases</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00318</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000224">
        <property type="protein sequence ID" value="CCZ84411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00318"/>
    <dbReference type="EnsemblBacteria" id="CCZ84411">
        <property type="protein sequence ID" value="CCZ84411"/>
        <property type="gene ID" value="BN566_00318"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="2">
        <location>
            <begin position="52"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="388" mass="42385" checksum="BA18D575CEBC34F3" modified="2013-07-24" version="1">MTIYNAVIHTMTEQGTIPHGFLEVENGKITCVQSGDPDTVTEGDLDAKGSLLLPGFIDAHTHLGILEDGLDFEGDDCNECTDPFTPHLRAIDGVNPLDRCFSEALAAGVTTVMTTPGSANPCGGTMLILKTAGNCVDDMKLTFGGIKFALGENPKSVYHGRDEMPFTRMATAAIIREGLYKAKRYLEQWEAVEEAEDQPDYDAKCEALLPLLRREYKAHFHCHRADDMMTAIRIAKEFHLDAVLVHGTEGHRIPDIIQRAGVPIICGPILCDRCKPELRDLEITTPNILYQHGVSIALCTDHTVIPIQYLATTAALAVKGGLPYEAALEAITVQPAKILGVEDRVGSLKVGMDADLQLYHGNPLDLQCDPWLVMVDGKVCKGQEYFAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>R5VLV2</accession>
    <name>R5VLV2_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 9">Acyl carrier protein</fullName>
            <shortName evidence="7">ACP</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">acpP</name>
        <name evidence="11" type="ORF">BN566_00395</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 9">Carrier of the growing fatty acid chain in fatty acid biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7 9">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="7">4'-phosphopantetheine is transferred from CoA to a specific serine of apo-ACP by AcpS. This modification is essential for activity because fatty acids are bound in thioester linkage to the sulfhydryl of the prosthetic group.</text>
    </comment>
    <comment type="PTM">
        <text evidence="9">4'-phosphopantetheine is transferred from CoA to a specific serine of apo-ACP by acpS.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the acyl carrier protein (ACP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000240">
        <property type="protein sequence ID" value="CCZ84496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00395"/>
    <dbReference type="EnsemblBacteria" id="CCZ84496">
        <property type="protein sequence ID" value="CCZ84496"/>
        <property type="gene ID" value="BN566_00395"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000036">
        <property type="term" value="F:acyl carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01217">
        <property type="entry name" value="Acyl_carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003231">
        <property type="entry name" value="Acyl_carrier"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006162">
        <property type="entry name" value="Ppantetheine_attach_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20863">
        <property type="entry name" value="PTHR20863"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="SSF47336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00517">
        <property type="entry name" value="acyl_carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00012">
        <property type="entry name" value="PHOSPHOPANTETHEINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="4 7" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 7" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5 7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="1 7 8" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="3 7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carrier" evidence="10">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-(pantetheine 4'-phosphoryl)serine" evidence="7 8">
        <location>
            <position position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ84496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="78" mass="8725" checksum="A1ED4F18EFD2E8C7" modified="2013-07-24" version="1">MTTFDKIKDMIVDQLDVEADEVTMDANIQEDLGADSLDIVDLIMAVEDEFEVKIDDDASENIKTVGDIVKFIDANKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>R5VLY7</accession>
    <name>R5VLY7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">50S ribosomal protein L6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rplF</name>
        <name evidence="8" type="ORF">BN566_00072</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 5">This protein binds to the 23S rRNA, and is important in its secondary structure. It is located near the subunit interface in the base of the L7/L12 stalk, and near the tRNA binding site of the peptidyltransferase center.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the universal ribosomal protein uL6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000241">
        <property type="protein sequence ID" value="CCZ84526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00072"/>
    <dbReference type="EnsemblBacteria" id="CCZ84526">
        <property type="protein sequence ID" value="CCZ84526"/>
        <property type="gene ID" value="BN566_00072"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.930.12">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01365_B">
        <property type="entry name" value="Ribosomal_L6_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000702">
        <property type="entry name" value="Ribosomal_L6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020040">
        <property type="entry name" value="Ribosomal_L6_a/b-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036789">
        <property type="entry name" value="Ribosomal_L6_a/b-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019906">
        <property type="entry name" value="Ribosomal_L6_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002358">
        <property type="entry name" value="Ribosomal_L6_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655">
        <property type="entry name" value="PTHR11655"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655:SF14">
        <property type="entry name" value="PTHR11655:SF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00347">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002162">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00059">
        <property type="entry name" value="RIBOSOMALL6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56053">
        <property type="entry name" value="SSF56053"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03654">
        <property type="entry name" value="L6_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00525">
        <property type="entry name" value="RIBOSOMAL_L6_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3 4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 5" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Ribosomal_L6" evidence="7">
        <location>
            <begin position="11"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal_L6" evidence="7">
        <location>
            <begin position="91"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="150"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="150"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ84526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="181" mass="19602" checksum="26BF739F732BACF1" modified="2013-07-24" version="1">MSRIGKKPISVPAGVTVTCENNHVTVKGPKGEIAKQFSPELGIAVEDGVITVTRPTDDKEHRSLHGLTRTLIANMVEGVTNGYSKTLIIEGVGYRAAKKGKEVVLSVGFSHPVNVPETDDIKLDVPQPNTIVVSGIDKQAVGQYAAEIREKRPPEPYKGKGIRYENEHIIRKEGKAGKGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>R5VM20</accession>
    <name>R5VM20_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribonuclease Y</fullName>
            <shortName evidence="5">RNase Y</shortName>
            <ecNumber evidence="5">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rny</name>
        <name evidence="9" type="ORF">BN566_00437</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Endoribonuclease that initiates mRNA decay.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the RNase Y family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="5"/>
    <dbReference type="EMBL" id="CBAR010000247">
        <property type="protein sequence ID" value="CCZ84576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00437"/>
    <dbReference type="EnsemblBacteria" id="CCZ84576">
        <property type="protein sequence ID" value="CCZ84576"/>
        <property type="gene ID" value="BN566_00437"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00335">
        <property type="entry name" value="RNase_Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006675">
        <property type="entry name" value="HDIG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017705">
        <property type="entry name" value="Ribonuclease_Y"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022711">
        <property type="entry name" value="RNase_Y_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12826:SF15">
        <property type="entry name" value="PTHR12826:SF15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12072">
        <property type="entry name" value="RNase_Y_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="SSF54791"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00277">
        <property type="entry name" value="HDIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03319">
        <property type="entry name" value="RNase_Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004395261" description="Ribonuclease Y" evidence="7">
        <location>
            <begin position="26"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="domain" description="HD" evidence="8">
        <location>
            <begin position="345"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="35"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="86"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ84576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="529" mass="59900" checksum="240ABDD6F596C84E" modified="2013-07-24" version="1">MEIVIVCLVACVICVAAVLVTNHTATQKGYTKGVQDGIQQRKQQAEAEIGSAEKEAARVIHDAKKEAEQQKKSVLLEAKDEVFQLRADAEKEIKDRRSEVSRQERRVQQKEESLDKKLENLERKEDNLARKQKSVEERLAEAEAIKNSQMDVLERISEFTREQAKEYILQHLEDELVHEKAMKITQYEQQLKDDCEEKARNYISLAIAKCASDQVSESAVSVVPLPNDEMKGRIIGREGRNIRTLETLTGVDLIIDDTPEAITLSCFDQVRREIARIALEKLIADGRIHPTKIEEMVEKAKREVEQRIRVEGEHAVLETNVHGINNEMIKLLGRLYYRTSYRQNVLKHSIEVSKLCGVLASELGLDANMARRAGLLHDIGKALTAEIEGSHVQIGVDVCKKYNESPEIIHAVEAHHNDVEPRTALACIVQAADAISAARPAARSENYENYIKRLQQLENICASYDGVEKCYALQAGREVRVMVVPEKVNDEKMVLIARQIAKQIENEMNYPGQVKINLIRESRVVDYAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5VM71</accession>
    <name>R5VM71_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphomethylpyrimidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00494</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ThiD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000257">
        <property type="protein sequence ID" value="CCZ84641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00494"/>
    <dbReference type="EnsemblBacteria" id="CCZ84641">
        <property type="protein sequence ID" value="CCZ84641"/>
        <property type="gene ID" value="BN566_00494"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008972">
        <property type="term" value="F:phosphomethylpyrimidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01169">
        <property type="entry name" value="HMPP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045029">
        <property type="entry name" value="HMP/HMP-P_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004399">
        <property type="entry name" value="HMP/HMP-P_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013749">
        <property type="entry name" value="PM/HMP-P_kinase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20858">
        <property type="entry name" value="PTHR20858"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08543">
        <property type="entry name" value="Phos_pyr_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00097">
        <property type="entry name" value="HMP-P_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phos_pyr_kin" evidence="2">
        <location>
            <begin position="14"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="262" mass="27523" checksum="FAA5335EB012120C" modified="2013-07-24" version="1">MIERVPVLTIAGSDSCGGAGIQADLKTMTALYVYGMSAITALTAQNTLGVQGILPVSADFLGQQLDAICTDIPPEAVKIGMIASPEQVDIICAKIQQYHLKKVVVDPVMVATTGAELTERTTVDAMREQLFAQADLLTPNLSETAVLLGKAVQTVEEMQAAAKQLAEQYQTAVLVKGGHLTEDAIDVLYDGSTMQCFSGARIATKNTHGTGCTLSSAIAANWAKGHSLSESVQQAKLYVANCIQAGLQIGHGNGAICHNYAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5VMC6</accession>
    <name>R5VMC6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in bacteria</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00554</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000272">
        <property type="protein sequence ID" value="CCZ84701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00554"/>
    <dbReference type="EnsemblBacteria" id="CCZ84701">
        <property type="protein sequence ID" value="CCZ84701"/>
        <property type="gene ID" value="BN566_00554"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036882">
        <property type="entry name" value="Alba-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007347">
        <property type="entry name" value="SpoVS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35331">
        <property type="entry name" value="PTHR35331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04232">
        <property type="entry name" value="SpoVS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="86" mass="8928" checksum="61BA4B10EC14E356" modified="2013-07-24" version="1">MEILKVSSHSSPNSVAGAIAGVIREKKMVEVQAVGAGAANQAVKAIAIARGYLAPIGVDLVCIPAFANILIDGEERTAIKLICEER</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5VQS8</accession>
    <name>R5VQS8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Type I restriction-modification system methyltransferase subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00717</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000029">
        <property type="protein sequence ID" value="CCZ82917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00717"/>
    <dbReference type="EnsemblBacteria" id="CCZ82917">
        <property type="protein sequence ID" value="CCZ82917"/>
        <property type="gene ID" value="BN566_00717"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4080.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005502">
        <property type="entry name" value="Ribosyl_crysJ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036705">
        <property type="entry name" value="Ribosyl_crysJ1_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03747">
        <property type="entry name" value="ADP_ribosyl_GH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101478">
        <property type="entry name" value="SSF101478"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ82917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="267" mass="28924" checksum="F5EE4D8A4322D97F" modified="2013-07-24" version="1">MIGAIIGDIVGSRFEWNNYRKKDFTLFTEDCFATDDSIMSLAIAGAVLQHQTEAVDLSKAAVCWMQKVGRPYPHCGFGGNFYHWIYSDDPKPYNSFGNGAAMRVSACGLSAKTLEAALQMSTAVTAVTHNHPEGIKGANATTAAIFLAKSGASKDAIRSHIVENYYPLSFTIDAIRPDYGFNETCQDTVPQAIEAFLESESFEDAIRTAISVGGDSDTLAAITGSIAEAYYGVPKDLYDTAVTYLDEPLKQILFAFESVFGSNLQAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5VRC4</accession>
    <name>R5VRC4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00894</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000059">
        <property type="protein sequence ID" value="CCZ83107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00894"/>
    <dbReference type="EnsemblBacteria" id="CCZ83107">
        <property type="protein sequence ID" value="CCZ83107"/>
        <property type="gene ID" value="BN566_00894"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="86" mass="9869" checksum="6AD64DC928251161" modified="2013-07-24" version="1">MEEIRGLYIYESDDADSGEFTYFCMAPDTMATTWHIEFRYGSDLDALKQYDAGEYAYWLASGISVDCSEEDINNCINLFCTENLSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5VSQ5</accession>
    <name>R5VSQ5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_01325</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000130">
        <property type="protein sequence ID" value="CCZ83557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01325"/>
    <dbReference type="EnsemblBacteria" id="CCZ83557">
        <property type="protein sequence ID" value="CCZ83557"/>
        <property type="gene ID" value="BN566_01325"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01046">
        <property type="entry name" value="Rubrerythrin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009040">
        <property type="entry name" value="Ferritin-like_diiron"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024934">
        <property type="entry name" value="Rubredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003251">
        <property type="entry name" value="Rubrerythrin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045236">
        <property type="entry name" value="Rubrerythrin-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02915">
        <property type="entry name" value="Rubrerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50905">
        <property type="entry name" value="FERRITIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50903">
        <property type="entry name" value="RUBREDOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Rubredoxin-like" evidence="4">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="Ferritin-like diiron" evidence="5">
        <location>
            <begin position="52"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ83557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="182" mass="20034" checksum="278450C6D0B7CEAA" modified="2013-07-24" version="1">MKKFVCQVCGYIYEGENPPAACPVCKAPASKFVEQKEEKTEWADQHFVGVAKDVDPEIVEGLRQNFEGECSEVGMYLAMARVAHREGYPEIGLYWEKAAYEEAEHAAKFAELLGEVVTDSTKKNLEMRVAAESGACAGKKALAAKAKALNLDAIHDTVHEMAKDEARHGCAFAGLLKRYFGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5VT06</accession>
    <name>R5VT06_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phage portal protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01408</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000148">
        <property type="protein sequence ID" value="CCZ83672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01408"/>
    <dbReference type="EnsemblBacteria" id="CCZ83672">
        <property type="protein sequence ID" value="CCZ83672"/>
        <property type="gene ID" value="BN566_01408"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006944">
        <property type="entry name" value="Phage/GTA_portal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006427">
        <property type="entry name" value="Portal_HK97"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04860">
        <property type="entry name" value="Phage_portal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01537">
        <property type="entry name" value="portal_HK97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="395" mass="44300" checksum="6398772A8DE0422A" modified="2013-07-24" version="1">MFWNQKKKTRNNSPVALFLSDRENDAICVPGYTTLDHCPEVMTACRRIAELIGSLTIHLMENTEQGDKRIVNALSRKIDIEPMANMTRKTWMEAIVMNLLLYGKGNSIVKVHTTGGYLRDLEPIAASKVSIPESGSYSVMIDGIPYKSDEILHFVHNPSPNCLWKGRGLQISLRPFADNLKQAAATEKSFLSSKWKPSVIVKVDALTDEFSSPTGRKKLLADYVESSEVGEPWLIPAEQFSIEQIKPLSLSDLAISDVVKLNRRMIAAILGVPPFLLGVDSYSKDEWNAFVNHTVKPIVIGIQQEMTKKLILSPNMYIRFNVLSLFDWDIKTIADVFGGLSDRGFATGNEVRDRMGLSPREGLDELRVLENYIPYEMSAYQKKLVQGGKEENGTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5VT83</accession>
    <name>R5VT83_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SpoU_sub_bind domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01487</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000161">
        <property type="protein sequence ID" value="CCZ83752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01487"/>
    <dbReference type="EnsemblBacteria" id="CCZ83752">
        <property type="protein sequence ID" value="CCZ83752"/>
        <property type="gene ID" value="BN566_01487"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="L30e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013123">
        <property type="entry name" value="SpoU_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08032">
        <property type="entry name" value="SpoU_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00967">
        <property type="entry name" value="SpoU_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="SSF55315"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SpoU_sub_bind" evidence="1">
        <location>
            <begin position="36"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="266" mass="29678" checksum="8268E5D0D6398CD7" modified="2013-07-24" version="1">MEFRMTAITAKDNPSIQLYRKLVRQRKARTQNQQFVLEGYRLVTDAVHFSRKLTHLFLTEAAWERYQEEFCSLEADLRETKRLLISNELAMELTETEHPQGVFAICQMPAEQVPIVAEGTYLVLYQLQDPGNMGMLLRTAAAMGVSGVICCQSCDFYSPKVVRATMGALFHVPVMQMPDAETVLQSLRAANIVSCASVVQGDAEQVGLCELGNGCAVWIGNEGNGLPESVVQQCERRITIPMKGYMESLNAAMAAGIMMWEMTKPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>R5VT87</accession>
    <name>R5VT87_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribonuclease 3</fullName>
            <ecNumber evidence="6">3.1.26.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Ribonuclease III</fullName>
            <shortName evidence="6">RNase III</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rnc</name>
        <name evidence="10" type="ORF">BN566_01492</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. Processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ribonuclease III family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.3" evidence="6"/>
    <dbReference type="EMBL" id="CBAR010000161">
        <property type="protein sequence ID" value="CCZ83757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01492"/>
    <dbReference type="EnsemblBacteria" id="CCZ83757">
        <property type="protein sequence ID" value="CCZ83757"/>
        <property type="gene ID" value="BN566_01492"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00593">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00104">
        <property type="entry name" value="RNase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011907">
        <property type="entry name" value="RNase_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14622">
        <property type="entry name" value="Ribonucleas_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00535">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="SSF69065"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02191">
        <property type="entry name" value="RNaseIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00517">
        <property type="entry name" value="RNASE_3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="6" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="RNase III" evidence="9">
        <location>
            <begin position="6"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="8">
        <location>
            <begin position="160"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="123"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ83757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="231" mass="26224" checksum="3AD25369D98B2A52" modified="2013-07-24" version="1">MEQVHFEQFETEIRYHFKNRHFLVEALSHSSYANEKKKCRCSNERLEFLGDSVLSIVVSSYLFHQFPELPEGELTKLRASLVCEKALHVFAQEIHLGDYLLLGKGEEHTGGRMRSSILADAFEAVIAAIYLDGGMEAAERHIMRFIPKNLEQRESLGFRDYKTALQEVIQKNPEEKVEYVLVSESGPDHAKAFQVEVCLNSNVIGVGTGRSKKAAEQMAAKEALRLMGYEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5VTA8</accession>
    <name>R5VTA8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sulfide dehydrogenase (Flavoprotein) subunit SudA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01512</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000161">
        <property type="protein sequence ID" value="CCZ83777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01512"/>
    <dbReference type="EnsemblBacteria" id="CCZ83777">
        <property type="protein sequence ID" value="CCZ83777"/>
        <property type="gene ID" value="BN566_01512"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028261">
        <property type="entry name" value="DPD_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006004">
        <property type="entry name" value="Glut_synth_NADPH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14691">
        <property type="entry name" value="Fer4_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01316">
        <property type="entry name" value="gltA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fer4_20" evidence="2">
        <location>
            <begin position="23"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Pyr_redox_2" evidence="1">
        <location>
            <begin position="143"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="466" mass="49949" checksum="76EF3D9506A2CF26" modified="2013-07-24" version="1">MAKPNMALEKVPMPERDPKERATCFEEVAMGYTPEMAMEEATRCLNCKAKPCVAGCPVGVRIPEFIQQMAAGNFLEAYQIITSTNSLPAVCGRVCPQESQCEGKCVRGKKGQPVGIGRLERFAADYAREHGNLQAKPVAPNGHKVAVVGGGPAGLTCAGDLARLGYQVTIFEAFQVAGGVLMYGIPEFRLPKSIVQKEVKTLEELGVEIRPNMVIGKILSIDELMEDGYEAVFVGSGAGLPRFMGIPGESLVGVCSANEYLTRINLMKGYREKYDTPVIKSKAVAVVGGGNVAMDAARSALRMGAEHVYIVYRRSEAEMPARKEEVHHAKEEGVEFLNLNNPVEILGDEKTGRVNGMKCIKMELGEPDESGRRRPVAIPGSEYVLDVDTVIMSIGTSPNPLIRTTTAGLEANKRGCLIVNEDTMQTTKDGVYAGGDAVTGAATVILAMGAGKKAAASIDEYIKTKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5VTG3</accession>
    <name>R5VTG3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF218 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01557</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000165">
        <property type="protein sequence ID" value="CCZ83822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01557"/>
    <dbReference type="EnsemblBacteria" id="CCZ83822">
        <property type="protein sequence ID" value="CCZ83822"/>
        <property type="gene ID" value="BN566_01557"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06259">
        <property type="entry name" value="YdcF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003848">
        <property type="entry name" value="DUF218"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02698">
        <property type="entry name" value="DUF218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="DUF218" evidence="2">
        <location>
            <begin position="108"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="259" mass="29017" checksum="15EDD4508314FCA4" modified="2013-07-24" version="1">MTLSIRTLLYDVLLLLELGLFFLFFAPLFLRIFNAGNLFGMAVSAICFLLTLFHRPFGQLLSRIWGNLCGKCVLSILALLLTAGIAFAGFCSIKMVQYYQNPPQTPCTVVVLGCKVKGTVPSLMLQRRLEAAKDYLQQHPDIPCIVSGGKGTGEDISEAQAMKTYLTAAGIAADRILMEDRSTDTHENLLFSKQILEQQGLPNRITILTDGFHQYRAHRMATELGLESWSVSGKTRRILIPTYWVREWMALFEQFLGMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VTK4</accession>
    <name>R5VTK4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01592</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000167">
        <property type="protein sequence ID" value="CCZ83857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83857">
        <property type="protein sequence ID" value="CCZ83857"/>
        <property type="gene ID" value="BN566_01592"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="109" mass="12342" checksum="982A195B0F4257A1" modified="2013-07-24" version="1">MPQYTRQDGDAMRQDAIRRSKEMYHRAAIPQPTPQRNYDFLPLREESGAASGGMKKNPLDLGNLLGGWNPADLLEHLDKDQILILVLLVMLYKEGGDKKLMMALAYLLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5VTL6</accession>
    <name>R5VTL6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">50S ribosomal protein L7/L12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01596</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000169">
        <property type="protein sequence ID" value="CCZ83867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01596"/>
    <dbReference type="EnsemblBacteria" id="CCZ83867">
        <property type="protein sequence ID" value="CCZ83867"/>
        <property type="gene ID" value="BN566_01596"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000206">
        <property type="entry name" value="Ribosomal_L7/12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014719">
        <property type="entry name" value="Ribosomal_L7/12_C/ClpS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013823">
        <property type="entry name" value="Ribosomal_L7/L12_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45987">
        <property type="entry name" value="PTHR45987"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00542">
        <property type="entry name" value="Ribosomal_L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54736">
        <property type="entry name" value="SSF54736"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal_L12" evidence="3">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="55" mass="5577" checksum="EC9A93498E6FB232" modified="2013-07-24" version="1">MAVIKAAKDVLGLGLKEAKAVVESAPATIKEGVSKADAEALKAKLEEAGATIEIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5VTS0</accession>
    <name>R5VTS0_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Putative 3-methyladenine DNA glycosylase</fullName>
            <ecNumber evidence="5">3.2.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_01646</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the DNA glycosylase MPG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.-" evidence="5"/>
    <dbReference type="EMBL" id="CBAR010000172">
        <property type="protein sequence ID" value="CCZ83917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01646"/>
    <dbReference type="EnsemblBacteria" id="CCZ83917">
        <property type="protein sequence ID" value="CCZ83917"/>
        <property type="gene ID" value="BN566_01646"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003905">
        <property type="term" value="F:alkylbase DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00540">
        <property type="entry name" value="AAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.300.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00527">
        <property type="entry name" value="3MGH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011034">
        <property type="entry name" value="Formyl_transferase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003180">
        <property type="entry name" value="MPG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036995">
        <property type="entry name" value="MPG_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10429">
        <property type="entry name" value="PTHR10429"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02245">
        <property type="entry name" value="Pur_DNA_glyco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50486">
        <property type="entry name" value="SSF50486"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00567">
        <property type="entry name" value="3mg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4 5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ83917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="171" mass="19734" checksum="9AB62BA2AA97DDA2" modified="2013-07-24" version="1">MMERLGADFFHRDCLEVAPDLVGKLLIRQLPDGTILQERIAETEAYRGEEDEACHAHKGRTKRTELLYRESGLIYVYLCYGMHWLMNVITGEPEQPQGVLFRAGVVHDGPAKLTRYLQVDQQWNGDSFLTCSNLWVADDGFRSKIQTAERVGIGYAGEVWQKKQWRFLIQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5VTY1</accession>
    <name>R5VTY1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">PASTA domain./Protein kinase domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_01706</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000178">
        <property type="protein sequence ID" value="CCZ83977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01706"/>
    <dbReference type="EnsemblBacteria" id="CCZ83977">
        <property type="protein sequence ID" value="CCZ83977"/>
        <property type="gene ID" value="BN566_01706"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006468">
        <property type="term" value="P:protein phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005543">
        <property type="entry name" value="PASTA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03793">
        <property type="entry name" value="PASTA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00740">
        <property type="entry name" value="PASTA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51178">
        <property type="entry name" value="PASTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="424"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="47"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="PASTA" evidence="4">
        <location>
            <begin position="544"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="450"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="618"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="623"/>
            <end position="637"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="664" mass="74506" checksum="57BC070F79D7C3B5" modified="2013-07-24" version="1">MNEEKLCYGCMQRTVFTNGICSNCGYYENTKTDPLFLKPGTQLHSRYLVGTAISMNGEGITYLAYDYPNSCRVLLREYMPHGFCSRVADSSVIRVQQNCLPQYKVLMEEFTELHKSIAQLRGQIHVNPVTDLFTENNTTYVVMEFIEGISFVDYLKENAGELTWAQFSRMLPPFLTSLSLLHNVGVVHRAISPETIFVTDKNELYLTDFSISAVRTANTELECEVYSGYAAPEQYSASNRQGTWTDVYAVCAVLYRVLTGTMPTSAISRMENDNLTAPTLLNPNIPRHVSNVIMHGLSLVSNDRIQTITDLVTQLFDDTADDHFQQQNTTVFQNQQTPPIYTTEHYDIPNVNGNTGYTANYATQNTGYVPQQDYAEQPQEYTDDYAYDDYHTADYGKQDYGKQSYQKEDAANTEAESVADRMRLPLIIGVLLLVILLIAVVLFIRGNLSSDDSSNSTAKVTTVATAENSSQPETNANSNANSVMPMLIGKNYENQAKQYQDWIRFQVEEVYSDQYEAGIIVWQEYEEGTYFDSSEPVKIKVSKGPANIDLPDWEGKSLDAYLQELNKLGVKYTTQGEENRSVTNGYVTRISQLPGVKYNLEEGAEITVYYADNPVETEAPTEAPTEAPQTEAPTEAPQTEAPTEAPATEPEPDSPIDGNIHFIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5VU45</accession>
    <name>R5VU45_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative cob(I)yrinic acid a c-diamide adenosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01753</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000180">
        <property type="protein sequence ID" value="CCZ84027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01753"/>
    <dbReference type="EnsemblBacteria" id="CCZ84027">
        <property type="protein sequence ID" value="CCZ84027"/>
        <property type="gene ID" value="BN566_01753"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008817">
        <property type="term" value="F:cob(I)yrinic acid a,c-diamide adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003724">
        <property type="entry name" value="CblAdoTrfase_CobA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02572">
        <property type="entry name" value="CobA_CobO_BtuR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015617">
        <property type="entry name" value="Adensltrnsf_CobA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="171" mass="19126" checksum="F70F7409C6A61519" modified="2013-07-24" version="1">MIHIYCGDGKGKTTAAVGLAVRCAGAGGTVLFYQFMKPETSSERIALQQIHGITVLSGYSIHQFSFRMTPEEKAEAAIGYQKQWQSLLEQVNQTTYQMLILDELLSCISTGFLPLEMVLSGMEQLPKELEVVITGRNPDEKLLAIADYITEMKNIRHPYEKHIAARKGIEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5VU78</accession>
    <name>R5VU78_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pectate lyase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01793</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000185">
        <property type="protein sequence ID" value="CCZ84067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01793"/>
    <dbReference type="EnsemblBacteria" id="CCZ84067">
        <property type="protein sequence ID" value="CCZ84067"/>
        <property type="gene ID" value="BN566_01793"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002105">
        <property type="entry name" value="Dockerin_1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00404">
        <property type="entry name" value="Dockerin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dockerin" evidence="1">
        <location>
            <begin position="61"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="124" mass="13799" checksum="CFA34307544D187B" modified="2013-07-24" version="1">MENGANIQQWESNGNSCQKWQAKTETTTVTTKATTTTVTTTTTTKMTTATTDIVTTTTTEPPVISGDINADGKTNLADLVLLQKWLLRVPETKLINWQAGDFDKNQSLNGIDLIFMRRKLMEMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5VUC7</accession>
    <name>R5VUC7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">zinc_ribbon_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01843</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000187">
        <property type="protein sequence ID" value="CCZ84117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84117">
        <property type="protein sequence ID" value="CCZ84117"/>
        <property type="gene ID" value="BN566_01843"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026870">
        <property type="entry name" value="Zinc_ribbon_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13240">
        <property type="entry name" value="zinc_ribbon_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="66"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="zinc_ribbon_2" evidence="3">
        <location>
            <begin position="2"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="244" mass="27090" checksum="D69FB62EEB809806" modified="2013-07-24" version="1">MFCKNCGFQLKDTARFCPKCGTANTPPPIPPQPSNFTPQPNNFAPQQNSSFSPNAQSIAVKTKKPLIIVGVGIVILIVIIIAFCIGFTGHGAGGGSKGGYKKATEAAEAALTAEYTGDAAAMMKLLPAKGKEYWEDDKNLTEKQLQDYMQNRMNTYGHKYDEVSVKVDDNSNPIENSKVTERVNSNYLDNYGITADEVIYFAECELTVDGEERDEGNDVYVYKYNGRWYSIYPEYHLIEDTDSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5VUJ9</accession>
    <name>R5VUJ9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PS_pyruv_trans domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01927</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01927"/>
    <dbReference type="EnsemblBacteria" id="CCZ84202">
        <property type="protein sequence ID" value="CCZ84202"/>
        <property type="gene ID" value="BN566_01927"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007345">
        <property type="entry name" value="Polysacch_pyruvyl_Trfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04230">
        <property type="entry name" value="PS_pyruv_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="PS_pyruv_trans" evidence="2">
        <location>
            <begin position="93"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="385" mass="44648" checksum="C93F993BAC462E78" modified="2013-07-24" version="1">MKKNSMAYDTAVYSLWFGLNYGSILTACALYKTLEQYGKHPALLQKQPALWGEHYAEKDNIAGCFAYQNCDVLEVFDSEEDARILREDVQNHVVGSDVVWNYNVVGKQSGLYYFLENVPADKRKIAYASCFGGAFHAEGETRNDCGRLLHQFNGIAVKEYEEAEVLQDLFHITPEMVLDPVFLCDKQFYIDCAERSAAKAVETDQSFIFTYIECCDDRKRQFLLKGNDILLVNHYSQLRNFIDINRFPESKALLKLDPAYHIRVEDWLYYLIHSEFVITDNYYGMCFALIFEKPFVVLANMDLPDLYRYRAILQPLGLEERLVILQEDLKKKEYLFRKPIRYDRVNRVLQKMKQDSEVWLKQQLGIAVPDSAATTSNPQEQAVGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>R5VUL7</accession>
    <name>R5VUL7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Holliday junction ATP-dependent DNA helicase RuvB</fullName>
            <ecNumber evidence="8">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ruvB</name>
        <name evidence="11" type="ORF">BN566_01942</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Forms a complex with RuvA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the RuvB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="8"/>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01942"/>
    <dbReference type="EnsemblBacteria" id="CCZ84217">
        <property type="protein sequence ID" value="CCZ84217"/>
        <property type="gene ID" value="BN566_01942"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00016">
        <property type="entry name" value="DNA_helic_RuvB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041445">
        <property type="entry name" value="AAA_lid_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004605">
        <property type="entry name" value="DNA_helicase_Holl-junc_RuvB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008824">
        <property type="entry name" value="RuvB-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008823">
        <property type="entry name" value="RuvB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42848">
        <property type="entry name" value="PTHR42848"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17864">
        <property type="entry name" value="AAA_lid_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05491">
        <property type="entry name" value="RuvB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05496">
        <property type="entry name" value="RuvB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00635">
        <property type="entry name" value="ruvB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6 8" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="7 8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4 8 11" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0742">SOS response</keyword>
    <feature type="domain" description="AAA" evidence="10">
        <location>
            <begin position="60"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="68"/>
            <end position="75"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ84217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="363" mass="39907" checksum="9F6A2626A717B9DF" modified="2013-07-24" version="1">MIETSDMQFDSRMVTAEETPEDHDVEVTLRPQTLAEYIGQDKVKENMAIYIQAAKRRGDPLDHVLLYGPPGLGKTTLSAIIAHEMGVNLRVTTGPAIEKPGDLAALLTNLQPNDVLFIDEIHRLSRAVEEILYPALEDHAIDIIMGKGPSARSLRIDLNPFTLIGATTRAGQLSSPLRDRFGVIQRLELYTTEQLTDIVRRSSMILEIPCTFEGAAEIASRARGTPRIANRLLKRVRDFADVMGNGEITLDIAKIALGRLEIDELGLDSNDRRMLTMMIKGYGGGPVGLETLASALGEEAVTLEDVCEPYLMQLGFLSRTPRGRCATALAYEHMGFPLPEQRKAPIQGQMTLSEAEADAAEQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5VUM3</accession>
    <name>R5VUM3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Energy-coupling factor transporter transmembrane protein EcfT</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_01947</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01947"/>
    <dbReference type="EnsemblBacteria" id="CCZ84222">
        <property type="protein sequence ID" value="CCZ84222"/>
        <property type="gene ID" value="BN566_01947"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003339">
        <property type="entry name" value="ABC/ECF_trnsptr_transmembrane"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02361">
        <property type="entry name" value="CbiQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="49"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="74"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="150"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="247"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ84222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="268" mass="30104" checksum="C5119B732DAFFE9E" modified="2013-07-24" version="1">MLKDITIGQYYPGNSFVHRLDPRFKIIITLLYITMLFMGSHLYSMVFGIVFFFLTFFCSGIAAKVLLKSMKPILPILLITAILDLLFIRDGTVYWSLGFLQITQEGVKTGVQMIIRISFLIVGTSMLTYTTSPIALTDAIERLLSPLKKLKFPVHAFSMMMTIALRFIPTLIEETDKIIAAQKARGADMESGNLIQRAKALIPILVPLFVSAFRRAEELATAMECRCYRGDIGRTKMKQLHLSGKDWLAMSITLVFFAGSIAMKIILG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VUN2</accession>
    <name>R5VUN2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01957</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84232">
        <property type="protein sequence ID" value="CCZ84232"/>
        <property type="gene ID" value="BN566_01957"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="179" mass="20427" checksum="60D2BEABAAAD08B8" modified="2013-07-24" version="1">MGISSTLYSGYSKQEIETNRIPTPFGAATVSISGQPISFHYTTKQHIFKNGISTQIHRIRIDTLNYQLDDMLDIQVGTIRTFEFFDSDESTVMTEYHDDKFCIALIGYDTDYDFRTDAFYGGDVYSYDSSCTESGICCTIVRNPAVFSRKHNRMLYAWVVFLPVSDCYDAADMIDIELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5VUR4</accession>
    <name>R5VUR4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nitroreductase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00191</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000204">
        <property type="protein sequence ID" value="CCZ84262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00191"/>
    <dbReference type="EnsemblBacteria" id="CCZ84262">
        <property type="protein sequence ID" value="CCZ84262"/>
        <property type="gene ID" value="BN566_00191"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.109.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029479">
        <property type="entry name" value="Nitroreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000415">
        <property type="entry name" value="Nitroreductase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00881">
        <property type="entry name" value="Nitroreductase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55469">
        <property type="entry name" value="SSF55469"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitroreductase" evidence="1">
        <location>
            <begin position="10"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="Nitroreductase" evidence="1">
        <location>
            <begin position="74"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="174" mass="19333" checksum="18280720C2D274AF" modified="2013-07-24" version="1">MEQTMLEAMMSRKSVRSYTDQMVSDADIEKVLKAGQYAANGRGRMATKFVVVKNREIRDELSKMNADIMGTTSDPFYGAPVVIWVLADRNVHTYVEDGSLAIGNMLLEAHALGLGACWIHRAKEMSELPEGRALLQKWGVSDDEIGVGCCILGYAKGEQPEKKPRRNDQIIVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5VUY0</accession>
    <name>R5VUY0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00240</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000213">
        <property type="protein sequence ID" value="CCZ84327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84327">
        <property type="protein sequence ID" value="CCZ84327"/>
        <property type="gene ID" value="BN566_00240"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002105">
        <property type="entry name" value="Dockerin_1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00404">
        <property type="entry name" value="Dockerin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dockerin" evidence="2">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="38"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="91" mass="9768" checksum="D42071E046CD93AB" modified="2013-07-24" version="1">MGIYGDVDGNGIVDVSDAVAVLTYYAKRAAGLEPIFGETPEENEAIFALADVDQDGIITVQDAVYILTYYAQKASGMQPTWEELTGISALF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5VV93</accession>
    <name>R5VV93_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Glycosyl hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_00334</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 25 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000225">
        <property type="protein sequence ID" value="CCZ84427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00334"/>
    <dbReference type="EnsemblBacteria" id="CCZ84427">
        <property type="protein sequence ID" value="CCZ84427"/>
        <property type="gene ID" value="BN566_00334"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003796">
        <property type="term" value="F:lysozyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016998">
        <property type="term" value="P:cell wall macromolecule catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009253">
        <property type="term" value="P:peptidoglycan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002053">
        <property type="entry name" value="Glyco_hydro_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018077">
        <property type="entry name" value="Glyco_hydro_fam25_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01183">
        <property type="entry name" value="Glyco_hydro_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00641">
        <property type="entry name" value="Glyco_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51904">
        <property type="entry name" value="GLYCOSYL_HYDROL_F25_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="13"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ84427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="263" mass="30483" checksum="0807F64EB52C0F59" modified="2013-07-24" version="1">MQQKTRKKRYGKYILRWSITLLILVMLVFGSICGLRLRHFYRAIHGEIPAESPDLSKFPVKGIDISRYQGDIDWDVLSKEDHVQFAFIKATEGSTYQDDLFTQNWEAAETAGVFVGAYHFFRFESDGKEQADNFIATVPKLENTLPPVIDIEIYDGETVPDAKTARTEIQEMADALEAYYGVKPILYTNPSTYMDYLMGHRWGYAVWMSNPYCEPIVDWTFWQYSFEGDLQGFAQEHIPVDLNVYNGSRKQFLKQFSLQEISN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5UX25</accession>
    <name>R5UX25_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00099</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000013">
        <property type="protein sequence ID" value="CCZ82863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82863">
        <property type="protein sequence ID" value="CCZ82863"/>
        <property type="gene ID" value="BN566_00099"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ82863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="88" mass="10301" checksum="868455B3100A8828" modified="2013-07-24" version="1">MMKKEAYDRFMSKMSSEFGNVDSVKEFLLDAEELAKYGEKRVALENLLENLLENEIYISSELINLAEEAFSDNPTDYDNELIYDIKQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5UX55</accession>
    <name>R5UX55_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00690</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000022">
        <property type="protein sequence ID" value="CCZ82888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82888">
        <property type="protein sequence ID" value="CCZ82888"/>
        <property type="gene ID" value="BN566_00690"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="46" mass="4545" checksum="D9A4B3A85A36C899" modified="2013-07-24" version="1">MCGCDGNNGCWWIIILIVLFVCCGCGGYGNCGGGCGCNNNGCGCGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXF9</accession>
    <name>R5UXF9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00105</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000035">
        <property type="protein sequence ID" value="CCZ82983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82983">
        <property type="protein sequence ID" value="CCZ82983"/>
        <property type="gene ID" value="BN566_00105"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="111" mass="12412" checksum="34637A9BC6042589" modified="2013-07-24" version="1">MGKIRYNRTGRTLVCPFGHECQFFDPRNNHWDSTPLLPVGIAAGITHRMLPDSLRMHLNSTGFQFEIAEIAHGKGGSCCEENISAEEAAPEEGTRFQKENGNFQRKKGFSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXK9</accession>
    <name>R5UXK9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">50S ribosomal protein L21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rplU</name>
        <name evidence="6" type="ORF">BN566_00806</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 5">This protein binds to 23S rRNA in the presence of protein L20.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 50S ribosomal subunit. Contacts protein L20.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the bacterial ribosomal protein bL21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000038">
        <property type="protein sequence ID" value="CCZ83013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00806"/>
    <dbReference type="EnsemblBacteria" id="CCZ83013">
        <property type="protein sequence ID" value="CCZ83013"/>
        <property type="gene ID" value="BN566_00806"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01363">
        <property type="entry name" value="Ribosomal_L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036164">
        <property type="entry name" value="L21-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028909">
        <property type="entry name" value="L21p-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001787">
        <property type="entry name" value="Ribosomal_L21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21349">
        <property type="entry name" value="PTHR21349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00829">
        <property type="entry name" value="Ribosomal_L21p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141091">
        <property type="entry name" value="SSF141091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00061">
        <property type="entry name" value="L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 5" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ83013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="103" mass="11276" checksum="C4FD954A4F0C796B" modified="2013-07-24" version="1">MYAVIETGGKQYQVKQGDVVFIEKLNAEEESTVTFDKVVAVSKEDGLTVGAPYVAGATVSAKVLKNGKAKKITVMTYKPKKSEKKKQGHRQPYTKVQIEAIEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXN9</accession>
    <name>R5UXN9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">N-acetylmuramoyl-L-alanine amidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00112</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000046">
        <property type="protein sequence ID" value="CCZ83058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00112"/>
    <dbReference type="EnsemblBacteria" id="CCZ83058">
        <property type="protein sequence ID" value="CCZ83058"/>
        <property type="gene ID" value="BN566_00112"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008745">
        <property type="term" value="F:N-acetylmuramoyl-L-alanine amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009253">
        <property type="term" value="P:peptidoglycan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06583">
        <property type="entry name" value="PGRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036505">
        <property type="entry name" value="Amidase/PGRP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002502">
        <property type="entry name" value="Amidase_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01510">
        <property type="entry name" value="Amidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00644">
        <property type="entry name" value="Ami_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55846">
        <property type="entry name" value="SSF55846"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetylmuramoyl-L-alanine amidase" evidence="1">
        <location>
            <begin position="31"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="210" mass="24158" checksum="BADE3D1D1DCE7855" modified="2013-07-24" version="1">MLLRPDFTFYLKGIRVRRYDLTQHNPNAIPMPTAVMDRDSIVGITIHNTNRKPSLYSMGTAEQCVWKTMHQELKDVRVHFYVDEYGAWQTLPLQLAGWHATDGAGNGNRRTIAIECAMSPNNTIEDRLSENNCAKLAACLLIWMNLPKAALFTHTYWLNCRDGIIGSREELNTHPHPYKMCPSYILPHWDAFEERVWAYYDQMLATIAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXU2</accession>
    <name>R5UXU2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Glycoside hydrolase family 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_00697</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000022">
        <property type="protein sequence ID" value="CCZ82895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00697"/>
    <dbReference type="EnsemblBacteria" id="CCZ82895">
        <property type="protein sequence ID" value="CCZ82895"/>
        <property type="gene ID" value="BN566_00697"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002105">
        <property type="entry name" value="Dockerin_1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018087">
        <property type="entry name" value="Glyco_hydro_5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00404">
        <property type="entry name" value="Dockerin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00659">
        <property type="entry name" value="GLYCOSYL_HYDROL_F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="1" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="3 5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dockerin" evidence="7">
        <location>
            <begin position="546"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="507"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ82895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="617" mass="68446" checksum="6A4DF16B312FE1FB" modified="2013-07-24" version="1">MMLEGSFCHEIKKTITGSFAGRNDGIVCNSRXXXXALSATAAAIPSFTTISAQAEDTNNDDWLHAVGSRLYDKDGNEVWLTGANWFGFNCGENCLHYLWSGDVDDMLSEVADRGINVIRMPISTELLISWMNDTPNPVSSVSAENNPPYFVINPDFLNADGSMKNSMEIFDIIMQKCKKYGLKAFIDIHSPHTDNSGHNYNLWYGKETADGTMVTTDLWIETLTWLADKYKNDDTLIGYDLKNEPHGKGQEGATAAKWDGSTDENNWAYAATKCANSILDVNPNALIFIEGVEQSVKSDAYTWGQPDSKTDPPYIPAWWGGNLRGVRKYPVVPDSGTSQIVYSPHDYGPSVYNQTWFDKDFTEQTLLDDYWYDTWAYVNAEDIAPLLIGEWGGHMDNGKNQQWMELLRDYMINHHINHTFWCLNTNSGDTGGLWAGIGYDQAASKTNLTWDADKYALFEKSLWQTLETGKYIGLDHQKALGNNGTGLSLSEFYASYASTEGSNLDGGTIVDGNTTKPTTDTTKPSTTTTTITTTTTAAATTTEAPKTDVLGDINDDQNVTISDLVLLNRYLLRKIDGTDAAYAFDRGDVNGDKILNIVDATLYRQYLLGTLKKFPAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXY1</accession>
    <name>R5UXY1_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">2-isopropylmalate synthase</fullName>
            <ecNumber evidence="3">2.3.3.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Alpha-IPM synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Alpha-isopropylmalate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">leuA</name>
        <name evidence="5" type="ORF">BN566_00725</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>3-methyl-2-oxobutanoate + acetyl-CoA + H2O = (2S)-2-isopropylmalate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21524"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.13" evidence="3"/>
    <dbReference type="EMBL" id="CBAR010000029">
        <property type="protein sequence ID" value="CCZ82925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00725"/>
    <dbReference type="EnsemblBacteria" id="CCZ82925">
        <property type="protein sequence ID" value="CCZ82925"/>
        <property type="gene ID" value="BN566_00725"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00070"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003852">
        <property type="term" value="F:2-isopropylmalate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07942">
        <property type="entry name" value="DRE_TIM_LeuA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00572">
        <property type="entry name" value="LeuA_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013709">
        <property type="entry name" value="2-isopropylmalate_synth_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002034">
        <property type="entry name" value="AIPM/Hcit_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005668">
        <property type="entry name" value="IPM_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036230">
        <property type="entry name" value="LeuA_allosteric_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039371">
        <property type="entry name" value="LeuA_N_DRE-TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08502">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00917">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110921">
        <property type="entry name" value="SSF110921"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00815">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00816">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 3" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0432">Leucine biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="4">
        <location>
            <begin position="32"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ82925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="558" mass="62756" checksum="0A8355D65258109B" modified="2013-07-24" version="1">MMKNANKYTRQFFPVNHPPMRWATKNYLDKPPIWCSVDLRDGNQALITPMSLEEKLEFFKVLVDIGFKEIEVGFPAASETEYEFCRTLIEQNLIPDDVTIQVLTQAREHIIAKTFEALQGVKHAIVHLYNSTSVAQREQVFKKSKEEVLNLAVTGAELCKKYRAMTPGHFTFEYSPESFTGTEPEYALEVCNAVLDVWQPTPDDKVIINLPVTVSHSMPHVYANQVEFMSDNLKYRENVYVSLHPHNDRGCAVADTEMGLLAGGDRVEGTLFGNGERTGNVDIITVALNMYSQGIDPQLDFSNLPAITEVYERVTRMHVYERSPYSGQLVFAAFSGSHQDAIAKGMKWREQHDCEHWNVPYLPLDPHDVGRVYEADVIRINSQSGKGGIGYLMETRYGFNIPAGMRESFGYLVKGVSDHAHKELQPDEVLKIFTDSYVNIETPVSLPEIHYVQKANDEITASVTIDYDGRQEVVTADGNGRLDAVSNALKAYLGSDYALVTYTEHALEVRSSSKAVSYVALQKGKDLYWGVGIHSDIIRSSISALVSALNRMMNEQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXZ8</accession>
    <name>R5UXZ8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Stage 0 sporulation protein A homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_00740</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000029">
        <property type="protein sequence ID" value="CCZ82940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00740"/>
    <dbReference type="EnsemblBacteria" id="CCZ82940">
        <property type="protein sequence ID" value="CCZ82940"/>
        <property type="gene ID" value="BN566_00740"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005561">
        <property type="entry name" value="ANTAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008327">
        <property type="entry name" value="Sig_transdc_resp-reg_antiterm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03861">
        <property type="entry name" value="ANTAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036382">
        <property type="entry name" value="RR_antiterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01012">
        <property type="entry name" value="ANTAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50921">
        <property type="entry name" value="ANTAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="5"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="ANTAR" evidence="5">
        <location>
            <begin position="125"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ82940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="192" mass="21715" checksum="031FCD641ED879FA" modified="2013-07-24" version="1">MYTERTLLISGSEKGLDAIINFLKGCGYAAVSVVSSGDEARRMLQQDTFSLIVLNTPLKDEFGYQLSLQLTQNTCSGVVMLCKADMAEEMANKTISYGVLVVAKPLNRAALLQAIRMGKAMHGRMMHMKAENDKLQKKLEELRCVSRAKCILIEREQYTEEQAHRYIEKLAMDTRRNRREVAMEILSRYVGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5UY51</accession>
    <name>R5UY51_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative membrane protein insertion efficiency factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00107</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Could be involved in insertion of integral membrane proteins into the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0161 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000035">
        <property type="protein sequence ID" value="CCZ82985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00107"/>
    <dbReference type="EnsemblBacteria" id="CCZ82985">
        <property type="protein sequence ID" value="CCZ82985"/>
        <property type="gene ID" value="BN566_00107"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00386">
        <property type="entry name" value="UPF0161_YidD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002696">
        <property type="entry name" value="Membr_insert_effic_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33383">
        <property type="entry name" value="PTHR33383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01809">
        <property type="entry name" value="YidD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01234">
        <property type="entry name" value="Haemolytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00278">
        <property type="entry name" value="TIGR00278"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="85" mass="9927" checksum="7089262EE573D98A" modified="2013-07-24" version="1">MKTLLLLLIRFYRRVLSPLKKPCCRYYPTCSAYALQAVKRFGAWHGTGLAVRRILRCNPWSPGGFDAVPLTLPQKRHRWTKIKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYC2</accession>
    <name>R5UYC2_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">50S ribosomal protein L27</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpmA</name>
        <name evidence="7" type="ORF">BN566_00808</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the bacterial ribosomal protein bL27 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000038">
        <property type="protein sequence ID" value="CCZ83015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00808"/>
    <dbReference type="EnsemblBacteria" id="CCZ83015">
        <property type="protein sequence ID" value="CCZ83015"/>
        <property type="gene ID" value="BN566_00808"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00539">
        <property type="entry name" value="Ribosomal_L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001684">
        <property type="entry name" value="Ribosomal_L27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018261">
        <property type="entry name" value="Ribosomal_L27_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15893">
        <property type="entry name" value="PTHR15893"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01016">
        <property type="entry name" value="Ribosomal_L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00063">
        <property type="entry name" value="RIBOSOMALL27"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00062">
        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00831">
        <property type="entry name" value="RIBOSOMAL_L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="10"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ83015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="93" mass="10304" checksum="900B252027B21CFE" modified="2013-07-24" version="1">MLKISMQFFAHKKGSGSTKNGRDSESKRLGVKRADGQFVKAGNILVRQRGTHIHPGNGVQIGSDDTLFATVNGIVKFERWGRDRKKVSVYTAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYG6</accession>
    <name>R5UYG6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative sporulation initiation inhibitor protein Soj</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01139</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000093">
        <property type="protein sequence ID" value="CCZ83363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01139"/>
    <dbReference type="EnsemblBacteria" id="CCZ83363">
        <property type="protein sequence ID" value="CCZ83363"/>
        <property type="gene ID" value="BN566_01139"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025669">
        <property type="entry name" value="AAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13614">
        <property type="entry name" value="AAA_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_31" evidence="1">
        <location>
            <begin position="4"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="259" mass="28251" checksum="38A876C356E92BF0" modified="2013-07-24" version="1">MGITIAISNQKGGVGKSTTAYNLGACLALNHDKKVLLIDFDPQANLSEYLKYEPDGNPTMTQLIMSFYTGNPVTAETAQRAIRHSETAGVDYLPADINLANAETIMVTMISKEHILRRILTEDVIGAYDFVLIDCLPSLGTLLINALTAADRVLIPVQTQKFSMDGLQSLEALTQLVKANTNPKLNLIGVLPTMVDRTKVSRTAIETLNEKYGEMLFRTSISKSIEAAKSSENGTPLCLTGHKLGQEYDELAQEVLSRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYK9</accession>
    <name>R5UYK9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00902</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000059">
        <property type="protein sequence ID" value="CCZ83115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83115">
        <property type="protein sequence ID" value="CCZ83115"/>
        <property type="gene ID" value="BN566_00902"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038109">
        <property type="entry name" value="DNA_bind_recomb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011109">
        <property type="entry name" value="DNA_bind_recombinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025827">
        <property type="entry name" value="Zn_ribbon_recom_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07508">
        <property type="entry name" value="Recombinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13408">
        <property type="entry name" value="Zn_ribbon_recom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00857">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="SSF53041"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51737">
        <property type="entry name" value="RECOMBINASE_DNA_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="2">
        <location>
            <begin position="26"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Recombinase" evidence="3">
        <location>
            <begin position="183"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="390"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="509" mass="58507" checksum="5FECB2C9B92FD2F5" modified="2013-07-24" version="1">MPKVTVIPPSINRTTRQDISAPTRRKVAAYARVSTDFEEQLTSYEAQISYYTNYIQRNPDWEFVKIYTDEGISATSTKHREGFNSMIADALDGKIDLIITKSVSRFARNTVDSLTTIRKLKEHHVECFFEKENIWTFDSKGELLITIMSSLAQEESRSISENVTWGQRKRFADGKVSLPYAHFLGYRKGESGLPEIVPEEAEIVRYIYQRFIDGLTPYKIANELTAQGIPTPCGKEKWSASTVKSILTNEKYKGDALLQKKFTVDFLTKKQQINEGQVPQYYVENSHPAIITPEEFDFVQSEFQKRCVKPYSSTSIYATKIICGDCGSYFGAKVWHSNSKYRRVIYQCNSKFKGSHFCTTPHLYEPEIQEKFLQAFAQYFSQKDAVIKNCQFALNRLKKQESKKAELQDELVAVNEKLKDYIQHGGENFDALNAKYEELSAQLDAEEIAESDRKRRIAKMQKILLTLKKTDSVLETFDESVWNAVLENLTVFHDGSLVFLFRDGTEIKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYM9</accession>
    <name>R5UYM9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Acetyltransferase N-acetylglutamate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00927</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000063">
        <property type="protein sequence ID" value="CCZ83140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00927"/>
    <dbReference type="EnsemblBacteria" id="CCZ83140">
        <property type="protein sequence ID" value="CCZ83140"/>
        <property type="gene ID" value="BN566_00927"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="152" mass="17017" checksum="02D955A924565BC6" modified="2013-07-24" version="1">MAIHYQKLTETELSTFIDMRIQQLREEGATEDIDLKPALFDYYRRHLADGTFVSWLALDGATIIGTSGMSFVEKPPYFGCPSGKIGLLSSMFTHPNYRRQGIAKKLLSLVVAEAKAYGCGTVQITASDMGVLLYTDFGFQKNKNFMQLKLTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYP4</accession>
    <name>R5UYP4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01207</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000104">
        <property type="protein sequence ID" value="CCZ83433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83433">
        <property type="protein sequence ID" value="CCZ83433"/>
        <property type="gene ID" value="BN566_01207"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="185" mass="21808" checksum="CA810E635D31C049" modified="2013-07-24" version="1">MAQKSFSISQTPPYTIPAELYDEAYRLYQRKYLLWKNWILEGILTLLTVDFIVTAVKDPSNGLTYFLMVLCVVLLALLIFHPLRIRRRVMDAVKEMGELPYVAALSEQEIVIQTLTEGEQGEEIPPTVLEYDEKMQIWEAEPFFLICEGKERFYVLPKKALYDEQIFAMRRFLRQKLGKRFHSNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYR6</accession>
    <name>R5UYR6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01227</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000109">
        <property type="protein sequence ID" value="CCZ83453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83453">
        <property type="protein sequence ID" value="CCZ83453"/>
        <property type="gene ID" value="BN566_01227"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="122" mass="13441" checksum="3AEFC054A068147F" modified="2013-07-24" version="1">MPVYLAYDHTPAAQKATPFVVLNLQACQTEAPVFRSEQETVPFSATVTVTLLMPIETHAAELSGTFTAEILPVLMQQQNLQTVQLSAPKTDRLLNRQTITATCQLFGFFQSEIEQAAAKEDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYU6</accession>
    <name>R5UYU6_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phosphatidylglycerol lysyltransferase</fullName>
            <ecNumber evidence="6">2.3.2.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Lysylphosphatidylglycerol synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">mprF</name>
        <name evidence="7" type="ORF">BN566_01247</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the transfer of a lysyl group from L-lysyl-tRNA(Lys) to membrane-bound phosphatidylglycerol (PG), which produces lysylphosphatidylglycerol (LPG), a major component of the bacterial membrane with a positive net charge. LPG synthesis contributes to bacterial virulence as it is involved in the resistance mechanism against cationic antimicrobial peptides (CAMP) produces by the host's immune system (defensins, cathelicidins) and by the competing microorganisms.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol) + L-lysyl-tRNA(Lys) = 1,2-diacyl-sn-glycero-3-phospho-1'-(3'-O-L-lysyl)-sn-glycerol + tRNA(Lys)</text>
            <dbReference type="Rhea" id="RHEA:10668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9696"/>
            <dbReference type="Rhea" id="RHEA-COMP:9697"/>
            <dbReference type="ChEBI" id="CHEBI:64716"/>
            <dbReference type="ChEBI" id="CHEBI:75792"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78529"/>
            <dbReference type="EC" id="2.3.2.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the LPG synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.3" evidence="6"/>
    <dbReference type="EMBL" id="CBAR010000114">
        <property type="protein sequence ID" value="CCZ83478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01247"/>
    <dbReference type="EnsemblBacteria" id="CCZ83478">
        <property type="protein sequence ID" value="CCZ83478"/>
        <property type="gene ID" value="BN566_01247"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050071">
        <property type="term" value="F:lysyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022791">
        <property type="entry name" value="L-PG_synthase/AglD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03706">
        <property type="entry name" value="LPG_synthase_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0046">Antibiotic resistance</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="42"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="197"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="234"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ83478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="312" mass="34834" checksum="CDB244E880E11A8C" modified="2013-07-24" version="1">MQFFKKHMKQLGTIVTLLALFFVVWKMVTMDVDYGAFATPRAMAILVLSILLQALIFCYMTFPWLHLVRILSGKKISGKQALPVYTKANLMKYIPGNVFQYVARNKLASEANISHVDVALATVLDIGMSMLAPFLISLLLMRRQIFTVLAAYQKTFLLVLLIGIAAVCLFLALVRWKFRDKLHAYFEKYRRLLQKKTLGQLALAFLLYLLQSVLCVGLYALPLLGVVSVPAEKIPQFLGAYLFSWIVGFITPGSPGGIGIREAVMMLICGTFLDTPSIVLYAVMMRLASTCADVVAFGIGAGYQRIQQKKAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYY6</accession>
    <name>R5UYY6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein conserved in bacteria</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01041</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01041"/>
    <dbReference type="EnsemblBacteria" id="CCZ83260">
        <property type="protein sequence ID" value="CCZ83260"/>
        <property type="gene ID" value="BN566_01041"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007329">
        <property type="entry name" value="FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04205">
        <property type="entry name" value="FMN_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00900">
        <property type="entry name" value="FMN_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN_bind" evidence="2">
        <location>
            <begin position="68"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="26"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="146" mass="15843" checksum="E0B10AA41D2F7315" modified="2013-07-24" version="1">MQFWIKLCNCVLVVGLLCGYQAIQNHRTQEEEIAKLEAQLQQQAIAAEDTETVDSPYQDGVYEGEAQGYGGTVAVELTIENGKFTDLTVVSAEKEDAAYFDAASSLLDTILEEQSTDVDTVSGATFSSNGIIHATEDALRKAEKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZ14</accession>
    <name>R5UZ14_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01307</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000121">
        <property type="protein sequence ID" value="CCZ83538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01307"/>
    <dbReference type="EnsemblBacteria" id="CCZ83538">
        <property type="protein sequence ID" value="CCZ83538"/>
        <property type="gene ID" value="BN566_01307"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003768">
        <property type="entry name" value="ScpA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33969">
        <property type="entry name" value="PTHR33969"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02616">
        <property type="entry name" value="SMC_ScpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="248" mass="27784" checksum="D368262D827514E4" modified="2013-07-24" version="1">MQQLSFEINGVTTPMDVLLTLIAKHKLNIYDIAISQLLEQYLNYLADCQEQDLELAGAFLEMAARLIYLKTAALLPKPQEAQADKEDLQESLLLYARCKQAAAILAGRYKEQQPFCRQPMPLPEPQKTYLRTYPAEVLRSSFLSIGKKQLPPSKAAMQEKLQRVVSPKTVSVFSKIVWLMRKVSHAETVSLVTLYDDISDRSARVAVFLAVLELTKHGRIGISTDGTSLYRISRKAAAADTAPQEIMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZ20</accession>
    <name>R5UZ20_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN566_01317</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CBAR010000123">
        <property type="protein sequence ID" value="CCZ83548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01317"/>
    <dbReference type="EnsemblBacteria" id="CCZ83548">
        <property type="protein sequence ID" value="CCZ83548"/>
        <property type="gene ID" value="BN566_01317"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="9"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="317"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="8">
        <location>
            <begin position="360"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="55"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="146"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="66"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ83548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="586" mass="66675" checksum="F35BDD98626D75C7" modified="2013-07-24" version="1">MIRHVRRHFICFTMCILTGAVLLPMIALNVIPAFMSYNQNKTVLQQAVQNEIRLRSPKPSKQPDKQPSENDPFIPQTTTVVSGTTVSVSVTSTTTTTETTAGITTDAPAEQIQAPPETAVQGSHTKPAAMTTQPVMTKPAAPVVTTQNVQTQPNDPNPDYPPDDNHDNPPPYPDDWHRPPEQDPWWWYEHEHYNDENMSFNGCGIPGAAMFSYMPLTASLTTEEKKPQDINFPPKEEHDTDYFLAVLDQQGNMIETVQADWHDYTEEQVAALVAQVQKQHKQDGKYESLQYYAQDYQKGTIISFMDCSKDQKFLRQLLFISIVVFLLMESIVFGLTMMLTKRAMQPMQISFEKQKQFISDAGHELKTPLTIISANADILQDEIGENKWLDYIRMQTERMRILVDEMMTLTKMEHSSATATAERFNLSSAVETMALPFESQAFEQQKKFEIDIQPELYFQGNPEQVRRMIGILIDNAFKYSAEHGAIRVSLKEETSGRAVLEVYNTGKGIREDEKEKIFERFYRSDSSRARATGGYGLGLSIAKSVADAHKMKIEVNSEPEHWITFTVWFPAQKKLPEKEKNQNHAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZ53</accession>
    <name>R5UZ53_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Dockerin type I repeat./Ricin-type beta-trefoil lectin domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_01106</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000085">
        <property type="protein sequence ID" value="CCZ83325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01106"/>
    <dbReference type="EnsemblBacteria" id="CCZ83325">
        <property type="protein sequence ID" value="CCZ83325"/>
        <property type="gene ID" value="BN566_01106"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10318">
        <property type="entry name" value="RGL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00161">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002105">
        <property type="entry name" value="Dockerin_1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041624">
        <property type="entry name" value="RGI_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034641">
        <property type="entry name" value="RGL11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43118">
        <property type="entry name" value="PTHR43118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00404">
        <property type="entry name" value="Dockerin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18370">
        <property type="entry name" value="RGI_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14200">
        <property type="entry name" value="RicinB_lectin_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="SSF50370"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="SSF69318"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="1" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="5" id="KW-0430">Lectin</keyword>
    <keyword evidence="1" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004394815" evidence="2">
        <location>
            <begin position="37"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="domain" description="Ricin B-type lectin" evidence="3">
        <location>
            <begin position="692"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="4">
        <location>
            <begin position="1021"/>
            <end position="1089"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="1092" mass="118291" checksum="8B56A9D578033A75" modified="2013-07-24" version="1">MKISFRHGWAMLSAAVLSLTALPLSAVSSVTSLSAAAAAAPVVENLDRGIVAIKSGNGMLVSWRFLASDPTGTEFRLYRDDQLLMTSKGDEATCFYDESGSAASRYRVDSLVNGTVQTTSTCSLISGTDYFQIPMDVPTASDCTYSPNDCSVGDVDGDGVYELFVKWDPSNSKDNSQKGKTGNVYIDCYKLTGEKLWRIDMGKNIRAGAHYTQFLVADFDGDGYAEMTCKTADGTVDGTGKTIGDGSKDYRNSSGYILSGPEYYTLFDGRTGAALDTVNYVPDRGTVSKWGDKYGNRVDRFLGSVAYLDGVHPSAVTIRGYYTRMTATAYDVVNKKLVQRWKFDTGNDSSKPGYGDGNHNCMPADVDGDGKQEIILGSTCLDDNGKVLWCLNKGHGDALHVGDLLPERPGLEVWMCHEDKPYGVTLADAKTGEIIFHKDGTGDTGRCCADNVWAGNNGAEFWGLGNDVFDGNGNTLNCRRPAINFLSYWDGDFEREILDGYTDSPATISKMGADGKLTTLLTTDGYYTCNTTKGTPCLSADIFGDWREELIVRAADSKSIRIYCTPYTTGSRITTLMQDPQYRNQVAGQNISYNQPPHASFYLGSDQPIPARPVVQVNGNGGTVEPIEPPTPTRPTGAVMDTSVKYHFQNVGSGQYLEVDSGKAENGANVQQWGADTFDGHNSWKLIAAGDGYYYIRSCVGDGKTYFLDLANGKPDDGTNIGIWENTHSDAQLFKFVDNGDGTYTITTKATEDKACVAVASDSTDSGANIVQWTCNGKDSQKWKISVDTIKDGVELDENTCYMLKNVNSNLYLEVKDGAAQAGATVQQWGANGAAAHNVWHVKKINWGYYYIYSALGDGESFVLNVNNGDNAEPLTIAANNKQSTQYFKFVDNEDGTYTIVTRASKDLSCVEIAGAQTSSGAVAQQWAWNDNDCQKWVLEPVAYTLPSQTTTVTTTILTTTETTTTTKPATTTAKPVKTTVMKPVMTTAKPVETMTTKPITTTAPVTSDSTETTTETTYEYPRNYGDVNVDGKVDIADAIFLNKYLAGQIALSVAGTLNADCNASGDLDDADTTVLMAYVLMKITDLPYIQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZ74</accession>
    <name>R5UZ74_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyltransferases involved in cell wall biogenesis</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00155</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000146">
        <property type="protein sequence ID" value="CCZ83628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00155"/>
    <dbReference type="EnsemblBacteria" id="CCZ83628">
        <property type="protein sequence ID" value="CCZ83628"/>
        <property type="gene ID" value="BN566_00155"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_2-like" evidence="1">
        <location>
            <begin position="10"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="267" mass="31141" checksum="A65027EA8D0A345A" modified="2013-07-24" version="1">MTEEKKPLISVLMGVYNCKERSMLERSVRSIVEQTYPNWELILCDDGSTNAALLWMQNLAARDSRIRLLQNDRNLGLARTLNRCLSVANGAYLARQDDDDISKPDRFEKQIAYLESHADVDFVGSNCDLYTPDGGVSGCWKRPERPRKQDFLFNSPFIHGSILFRRRCFEKVSGYPVMEKIARYEDYMLFMQLYAAGLQGANLQECLYQYYFDSKTRRIPVRERWDEAIVRWRGFHMLNLMPKGLPYIGKPLMLAMLPPKLIHHMHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZA0</accession>
    <name>R5UZA0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01399</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000148">
        <property type="protein sequence ID" value="CCZ83663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83663">
        <property type="protein sequence ID" value="CCZ83663"/>
        <property type="gene ID" value="BN566_01399"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="205" mass="24434" checksum="0E69559138A7A8EB" modified="2013-07-24" version="1">MDMSKRYSERFKMQVAYDYFVDRCPVNALGDKYKITGDTVRYFCSERRGEYRDAIVRNWKDEEKRLRRAVSDYLSGKSAEIITQEYNICTRRLFRMVDTRCNSYLIEPPEFTAEELAKPKAFTLYRSLTREILKMDVQRRPVYGIRDAITGMWLQRLDKKGNMHPLLYLTVDNANAKLRTVKMIRGQHNAGNVDLRVQWYGWEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZF2</accession>
    <name>R5UZF2_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Endopeptidase La</fullName>
            <ecNumber evidence="3">3.4.21.53</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_01458</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>Hydrolysis of proteins in presence of ATP.</text>
            <dbReference type="EC" id="3.4.21.53"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase S16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.53" evidence="3"/>
    <dbReference type="EMBL" id="CBAR010000158">
        <property type="protein sequence ID" value="CCZ83723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01458"/>
    <dbReference type="EnsemblBacteria" id="CCZ83723">
        <property type="protein sequence ID" value="CCZ83723"/>
        <property type="gene ID" value="BN566_01458"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004815">
        <property type="entry name" value="Lon_bac/euk-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008269">
        <property type="entry name" value="Lon_proteolytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027065">
        <property type="entry name" value="Lon_Prtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008268">
        <property type="entry name" value="Peptidase_S16_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10046">
        <property type="entry name" value="PTHR10046"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05362">
        <property type="entry name" value="Lon_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00763">
        <property type="entry name" value="lon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51786">
        <property type="entry name" value="LON_PROTEOLYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01046">
        <property type="entry name" value="LON_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3 6" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Lon proteolytic" evidence="5">
        <location>
            <begin position="250"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="438"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="337"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ83723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="471" mass="51323" checksum="5F0CFFAAF530A88D" modified="2013-07-24" version="1">MMPEVTGQILCFVGPPGVGKTSIGKSIAKALGRNYERVSLGGIRDESDIRGHRKTYIGAMPGRIMDAMARAKSKNPLILLDEIDKMSNDFRGDPSAAMLEVLDSEQNQAFRDHYIEVPFDLSKTLFIATANTLDTIPAPLLDRMEVIELGSYNREEKFQIAKHHLLSKQMKKHGLKGTQFKVQDAVLYTLIDDYTREAGVRELERMLAALCRKAVKELVSGTCKRVTITTKNLTTYLGHKKYLPDDQSKQDTVGIVNGLAWTSVGGVLMPLETLVLNGKGMIELTGSLGDVMKESAKIAVSYVRSIAKQYHIPEDFYLKNDLHIHAPEGAVPKDGPSAGVTMVTAIVSALSGIPVRHDVAMTGEITLHGKVLPIGGLPEKAMAAYKAGLKTVIIPKRNEPDLEDVDETVRNGLEFVTAENLETVLKTALVSVPEPIQPETPIAEQPDEQPAVFSPKAAGTPAPETKTLIPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZN8</accession>
    <name>R5UZN8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Endoglucanase A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN566_01553</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000165">
        <property type="protein sequence ID" value="CCZ83818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01553"/>
    <dbReference type="EnsemblBacteria" id="CCZ83818">
        <property type="protein sequence ID" value="CCZ83818"/>
        <property type="gene ID" value="BN566_01553"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040753">
        <property type="entry name" value="DUF5620"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018087">
        <property type="entry name" value="Glyco_hydro_5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18522">
        <property type="entry name" value="DUF5620"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00659">
        <property type="entry name" value="GLYCOSYL_HYDROL_F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="1" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004406599" evidence="6">
        <location>
            <begin position="32"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="domain" description="DUF5620" evidence="8">
        <location>
            <begin position="99"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="DUF5620" evidence="8">
        <location>
            <begin position="238"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="Cellulase" evidence="7">
        <location>
            <begin position="409"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="29"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ83818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="718" mass="78340" checksum="4DEF2C18F5238F6E" modified="2013-07-24" version="1">MNLRPGKSMLSILLAGILTLSALSGCSDKQESSSSASSAAQTTTAATEAETEANADAETTAPETTEATETTTTHISPTETVSSTLGAELDDLNVLLDRDADNTYKAKLENFVQDGDVIQSFTFIFYAPDGVSNMGTYKGGCGISVKADSATATDKNWYQSDDFEQSVNGAYAEITWNVPTDVAADVDANGDVLIGYWWSDLQQLKLSSIVCTYTRTAEVPVDGTSESSPVATLDYSSDTDKQVKLPLSDFIDKDDRLQTVTFDISSSGSLGKFTGAFGVSLTSGCDAETDKNWYQTGNIVTFTQDSSVSLTWIVPESIQDYIDPTGDLMLGYWWSDQATVTLNKVSVRYSNATGTVKKSDTKKKEEAQTAVKNEGAAAVSSEEVNQMSSSEIVADMKVGWNLGNTLDSYDTSSSDNETGWGNPKTTKAMIDTVKDAGFNAVRIPVTWGEHLSADNTIDADYLNRVQEIVDYAIDDNLYVILNVHHDDALWLHPTYSEQAAVTEKLTSIWKQLCERFGDYDHHLVFEGMNEPRVIGSATEWSGGTPEERDVINQLYQAFVDTVRATGGLNADRTLIISSHAQSITKEAVSAVKIPDDAHIAVSIHSYAPWDFCGTDDTRSDWGSDADKQELDTNFQYLEDTFISKGVPVLLDEFGAVNKNNTSIRAQYYAYYVKSAKAHGITCFVWDNGTESEFGLLNRKDLTWYAPSIIDAMMQALQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZX8</accession>
    <name>R5UZX8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Adenosylhomocysteine nucleosidase</fullName>
            <ecNumber evidence="2">3.2.2.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_01294</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (hydrolase route): step 1/2.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.9" evidence="2"/>
    <dbReference type="EMBL" id="CBAR010000121">
        <property type="protein sequence ID" value="CCZ83525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01294"/>
    <dbReference type="EnsemblBacteria" id="CCZ83525">
        <property type="protein sequence ID" value="CCZ83525"/>
        <property type="gene ID" value="BN566_01294"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00904">
        <property type="reaction ID" value="UER00871"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008782">
        <property type="term" value="F:adenosylhomocysteine nucleosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008930">
        <property type="term" value="F:methylthioadenosine nucleosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009164">
        <property type="term" value="P:nucleoside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010049">
        <property type="entry name" value="MTA_SAH_Nsdase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="SSF53167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01704">
        <property type="entry name" value="MTA/SAH-Nsdase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNP_UDP_1" evidence="6">
        <location>
            <begin position="4"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ83525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="227" mass="24749" checksum="AAFEB1227929C436" modified="2013-07-24" version="1">MSKRIGIIGAMPSELVDIRAALPNSNIIRKSGFDYYVNQLENGTEVIHVCSGIAKVNAAVCTQALIDNFQPDAIINAGIAGGMHRDVKVCDIVISDAVLPHDLDLHFLKDYPPYCGIFSADKHLMEVAAKTCDQLNMQHHFGSIVSGEAFISDNAVKQEIQERLHPMAVDMESAAVGHCCYLNEMPFVSIRCISDNADDEGAMSFDQFEKIAAKRVADLVLEIAAVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5V018</accession>
    <name>R5V018_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA repair protein RecN</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Recombination protein N</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_01667</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RecN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000174">
        <property type="protein sequence ID" value="CCZ83938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01667"/>
    <dbReference type="EnsemblBacteria" id="CCZ83938">
        <property type="protein sequence ID" value="CCZ83938"/>
        <property type="gene ID" value="BN566_01667"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004604">
        <property type="entry name" value="DNA_recomb/repair_RecN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11059">
        <property type="entry name" value="PTHR11059"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="44"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="167"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00033408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="439" mass="49291" checksum="C5BBFAACE85409EF" modified="2013-07-24" version="1">MGARLIHIHGQHDNQILRSADQHLQMIDQFGEMLPLLQSYQTHFRALQKIAKQLNAAQKAAKERKQKQAYLQTLLEEVRALQIEPGEEDRITEKMQQLQAAEDAAMAVRSAWQLLEENEQSATASLAEAQQQLQTATFPAADQPEQESWLQRLETVKVELLDLSESLFQKMEQLSDNEAQYQQLQQRAELFRQVSKTYLCTCDELLERANDAELQLEQMQHDAVAIKTLLEEKNHLLEVVSTEAKQLSQQRAAAARRFTDAVMEQLTFLNMPDVVLQVAHTTGKLTIQGMDSMELLISANKGEAPKPLAKIASGGELSRIMLALKCVIADRDQIPTLIFDEIDTGVSGKAAQKIGFKLKEVGKNRQVLCVTHLSQIAVMANHHLLIEKKVEGERTSTHVTPLDLEGRVQEIARIMAGDSPSKLMLQSAREALQQAQKAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5V034</accession>
    <name>R5V034_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_01682</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000175">
        <property type="protein sequence ID" value="CCZ83953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01682"/>
    <dbReference type="EnsemblBacteria" id="CCZ83953">
        <property type="protein sequence ID" value="CCZ83953"/>
        <property type="gene ID" value="BN566_01682"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17179">
        <property type="entry name" value="Fer4_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="224"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="300"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ83953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="336" mass="37294" checksum="2A3B5DB3C90AA7DF" modified="2013-07-24" version="1">MKKISLSKLDEFFAAVAASKNLYLPVDGAKGAEFKLWTEGTAMSKAYNTLRSAKDFFFPQVETLAAFKVSGKNIEVEDIRKETEDFVIFGVRACDAKSFEILDTVYLADPVDSYYASRREHGVVVTLSCKKPTETCFCKAFGIDATNPGGDVACWMTDDELLLQANTEKGEALLASLSLLEDAADDAADSEKEKLQTILDKLPLGNLSTESFGGDKLMALFNSDKWESLSESCLACGTCTFVCPTCQCYDIRDYDTGHGVQRFRCWDSCMYSDFTLCAHGNTRNTQMQRFRQRFMHKLVYYPANNNGVFGCVGCGRCLRSCPISMNIVKVMKALEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0C0</accession>
    <name>R5V0C0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Fibronectin type III domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01737</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000178">
        <property type="protein sequence ID" value="CCZ84008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84008">
        <property type="protein sequence ID" value="CCZ84008"/>
        <property type="gene ID" value="BN566_01737"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004394883" evidence="1">
        <location>
            <begin position="26"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="455" mass="51773" checksum="95EF5221B01DEB15" modified="2013-07-24" version="1">MKKIIAVLAAGGLLAASIPQQSVTAAETPALTDIVSLQKWILGESDITPENGQTWDWNADGTVNIADLCQMKRQYTTIPVQNPLDTLTGMDYQTAVANAYISKSEYGYQIAGNLKSTIEEKMGRPLDYSIDRFYLVNNKILGLDNSIKYLYNASTMDVYPVTEETKRNCATWYWKGSKAAVYGIDDDEEKQNEFLDALEWYGITEVYYSSGANKLVNNKDTVEKFVKNAYQRNMKVYLLTGEKTWLYEDTYQTAIYRVFDKVAEYNSMVDYDARLAGVSYDVEVWTNSDYNWKNNADVRAQQVKFVEAAQQYANEKNLSVIHCLPFWIVRYDYTDEDGTTKNVYDSITQIANDTILMTYRDSASAVERLVAEVQTNAEHPVLYYAEKNDCNLEIAVQVDQSKEGDSVSFYEEEQENPGCVKDALATIQTDLADYSEHTTFAIHQAIAMYEFYLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0G1</accession>
    <name>R5V0G1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative phosphatase C-terminal domain of histone macro H2A1 like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01799</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000186">
        <property type="protein sequence ID" value="CCZ84073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01799"/>
    <dbReference type="EnsemblBacteria" id="CCZ84073">
        <property type="protein sequence ID" value="CCZ84073"/>
        <property type="gene ID" value="BN566_01799"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002589">
        <property type="entry name" value="Macro_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01661">
        <property type="entry name" value="Macro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00506">
        <property type="entry name" value="A1pp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="SSF52949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51154">
        <property type="entry name" value="MACRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Macro" evidence="1">
        <location>
            <begin position="1"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="178" mass="19436" checksum="5D1085128D94FFE2" modified="2013-07-24" version="1">MSNLKLINDSCMEQEVDAIVNAANKYLAAGGGICGAIFNKAGYMELTHACSQVNTPLQDGEAAITPAFGIKNAKYIIHAVGPDFNATPEAFKELFEAYYHSLIVLKNSNLHSIAFPLISAGIFGGALANPVKESTKQCYQAYKQFCDKFPDYDVNVVLCAFSQKEMKEAQDVFKDFSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0G2</accession>
    <name>R5V0G2_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Signal recognition particle receptor FtsY</fullName>
            <shortName evidence="5">SRP receptor</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ftsY</name>
        <name evidence="8" type="ORF">BN566_01495</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Acts as a receptor for the complex formed by the signal recognition particle (SRP) and the ribosome-nascent chain (RNC).</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
            <orientation evidence="5">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the GTP-binding SRP family. FtsY subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000161">
        <property type="protein sequence ID" value="CCZ83760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01495"/>
    <dbReference type="EnsemblBacteria" id="CCZ83760">
        <property type="protein sequence ID" value="CCZ83760"/>
        <property type="gene ID" value="BN566_01495"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031226">
        <property type="term" value="C:intrinsic component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00920">
        <property type="entry name" value="FtsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004390">
        <property type="entry name" value="SR_rcpt_FtsY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036225">
        <property type="entry name" value="SRP/SRP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43134:SF7">
        <property type="entry name" value="PTHR43134:SF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47364">
        <property type="entry name" value="SSF47364"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00064">
        <property type="entry name" value="ftsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00300">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 5 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SRP54" evidence="7">
        <location>
            <begin position="510"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="51"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="97"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="146"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="343"/>
            <end position="350"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="425"/>
            <end position="429"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="489"/>
            <end position="492"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="542" mass="59901" checksum="E25BAC4C41319713" modified="2013-07-24" version="1">MGFFGRKKDKEKEKEKKGGLFGWMKRKQADEPEREEQIVEPEPETDTDDVAAQMLAEREAARQAETEQWREEAEAEIAADQAEAAALAAKAAQDELLTEEEDSEADAEDEDDEEPIAVTFQPEEAESEAEEAEPETVKVEPEAVAEPETVESEPEAVAESETVESELEEVAESETVETELEEVAEPETEGVEPEEVAEPEEVAEPETEESESEELDETEAEASEPEEPQEPEKKKKKGFFEKIRDGLRKTKDSVIAKMQLVLNAFTKIDEDLFDQLEETMIMGDMGAETSIEICDQLRKRVKERGITDPKQIMGLIQEIIGEMLGEDQTLQLQTKPSVIMVIGVNGAGKTTTIGKLCHQLKEDGKKVIVAAADTFRAAAIDQLEVWTDRAGVELVKHAEGSDPAAVVYDAIEAAKARNCDVLICDTAGRLHNKKNLMQELAKINRIIENKAAGCDKEILLVLDATTGQNAINQARLFKEVADITGIVLTKLDGTAKGGIIVSIKNELEIPVKLIGVGEKIDDLQPFHARDFVNALFETEERK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0I0</accession>
    <name>R5V0I0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01510</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000161">
        <property type="protein sequence ID" value="CCZ83775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01510"/>
    <dbReference type="EnsemblBacteria" id="CCZ83775">
        <property type="protein sequence ID" value="CCZ83775"/>
        <property type="gene ID" value="BN566_01510"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="163" mass="18410" checksum="00CC5791496A3894" modified="2013-07-24" version="1">MNLSDKMSYLKGLVDGMELDLTTKEGKVLGQLLDVLQETVLYVTDLQDQVDELTELCESLDEDLGDVEDAVFGDEEDDEDEADEFEDDDDDYDDDDDDDMYEAVCPNCQNTIVLSDSVLDEGTMHCPCCNELLEFDYSDLTMDDLEHKEEDAEDAESETEPEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0M1</accession>
    <name>R5V0M1_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Chorismate synthase</fullName>
            <shortName evidence="6">CS</shortName>
            <ecNumber evidence="6">4.2.3.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">5-enolpyruvylshikimate-3-phosphate phospholyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">aroC</name>
        <name evidence="7" type="ORF">BN566_01550</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the anti-1,4-elimination of the C-3 phosphate and the C-6 proR hydrogen from 5-enolpyruvylshikimate-3-phosphate (EPSP) to yield chorismate, which is the branch point compound that serves as the starting substrate for the three terminal pathways of aromatic amino acid biosynthesis. This reaction introduces a second double bond into the aromatic ring system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21020"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57701"/>
            <dbReference type="EC" id="4.2.3.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>FMNH2</name>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
        </cofactor>
        <text evidence="6">Reduced FMN (FMNH(2)).</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 7/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the chorismate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.5" evidence="6"/>
    <dbReference type="EMBL" id="CBAR010000165">
        <property type="protein sequence ID" value="CCZ83815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01550"/>
    <dbReference type="EnsemblBacteria" id="CCZ83815">
        <property type="protein sequence ID" value="CCZ83815"/>
        <property type="gene ID" value="BN566_01550"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00090"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004107">
        <property type="term" value="F:chorismate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd07304">
        <property type="entry name" value="Chorismate_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00300">
        <property type="entry name" value="Chorismate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000453">
        <property type="entry name" value="Chorismate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035904">
        <property type="entry name" value="Chorismate_synth_AroC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21085">
        <property type="entry name" value="PTHR21085"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01264">
        <property type="entry name" value="Chorismate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001456">
        <property type="entry name" value="Chorismate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103263">
        <property type="entry name" value="SSF103263"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00033">
        <property type="entry name" value="aroC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4 6" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2 6" id="KW-0288">FMN</keyword>
    <keyword evidence="5 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 6" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="125"/>
            <end position="127"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="305"/>
            <end position="309"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="332"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ83815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="363" mass="39171" checksum="1020CDD788B66B9E" modified="2013-07-24" version="1">MSSFWNNRISISIFGESHGTSIGVVMDNLPPGEYIDMEKVLQFMARRAPKKDGTTTPRSEKDLPQVQSGMLNNRTTGVPLCAIIQNTDTRSKDYSNLERLPRPGHADYTGAMRYRGFQDVRGGGHFSGRLTAPLCFAGAICGQILERRGIYTGAHIAAIHGVEDDAFSETDVSRADILAVREKPFAVINDAQGEIMQEDIRNAGKGGESLGGLIECAVVNMPAGVGSPMFQGLENTIAQLIFGIPAVKGLEFGAGFQVAEMVGSQNNDPFYMDENGHVKTKTNHHGGILGGISSGMPIILRVAIKPTASIAKPQETVDFRAGTNEILQIHGRHDPCIVPRAVPCVEAAVNLALLSHMMDYPHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0W7</accession>
    <name>R5V0W7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Methyltransferase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01644</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000172">
        <property type="protein sequence ID" value="CCZ83915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01644"/>
    <dbReference type="EnsemblBacteria" id="CCZ83915">
        <property type="protein sequence ID" value="CCZ83915"/>
        <property type="gene ID" value="BN566_01644"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016718">
        <property type="entry name" value="rRNA_m1G-MeTrfase_A_prd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018249">
        <property type="entry name" value="MyrA_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Methyltransf_11" evidence="3">
        <location>
            <begin position="90"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="6"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR018249-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR018249-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="274" mass="30457" checksum="A7B16B813F4DE334" modified="2013-07-24" version="1">MSVWRCPICAASMQHEEHSLHCLNGHCFDLAKSGYVNLLPVQQKHTKQPGDNLQMVRARRDFLQAGYYQPFQQALCAMVVQAMPQQGILLDAGCGDGYYTKAVTEALQAAGKAVHVYGVDISKYAVDLAAKQEKAATFAVGSVFHLPVSDHCCDGIFSLFAPYAGTEFQRVLKKHGTMILGIPGAKHLMGLKRAIYETPYENVVKPYDLEGFSFVGKQTVTGTIFLPNPTAIQNLFAMTPYYYKTGQKEQERLAALETLETEISFELLQYQRES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>R5V138</accession>
    <name>R5V138_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Regulatory protein RecX</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">recX</name>
        <name evidence="6" type="ORF">BN566_00221</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 5">Modulates RecA activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 5">Belongs to the RecX family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000211">
        <property type="protein sequence ID" value="CCZ84308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00221"/>
    <dbReference type="EnsemblBacteria" id="CCZ84308">
        <property type="protein sequence ID" value="CCZ84308"/>
        <property type="gene ID" value="BN566_00221"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006282">
        <property type="term" value="P:regulation of DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01114">
        <property type="entry name" value="RecX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003783">
        <property type="entry name" value="Regulatory_RecX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33602">
        <property type="entry name" value="PTHR33602"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02631">
        <property type="entry name" value="RecX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ84308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="207" mass="24272" checksum="DE81C4CC719530D2" modified="2013-07-24" version="1">MEITQVTQKSPSVWVLTLADGRSYEIDRTCVLEYALLNRLEIDDETLQEMQQTYCERHALQQALRWLNYRPYTFKAMFQRLMPIYGEATSFAVLKRLTEMGGINDYQYAQQMARSAVERKGYGLRRAAEMMRQKGVPKNVIAAVLQPYEETVPERLQQILERHYAEKLSDPSDRKMVERVKAALVRKGYSFSEIQAAVQRYWEETEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1A9</accession>
    <name>R5V1A9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_01741</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000180">
        <property type="protein sequence ID" value="CCZ84015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01741"/>
    <dbReference type="EnsemblBacteria" id="CCZ84015">
        <property type="protein sequence ID" value="CCZ84015"/>
        <property type="gene ID" value="BN566_01741"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="4"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="40"/>
            <end position="47"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ84015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="226" mass="24525" checksum="33234E3F2FC2B848" modified="2013-07-24" version="1">MEILSAKDLCKSYGSGENSVQALNHVSISVENGEFVAVIGTSGSGKSTLLNLLGGLDTPTSGEVLVSGKKLFQMNEEARTIFRRRNIGFVFQNYNLVPVLNVYENIVLPIELDGTKVDTEYVKTILHTLGLEQKVQAMPNQLSGGQQQRVAIARALAAKPAILLADEPTGNLDSKTGMDVIALLKMTSQAFHQTIVMITHNEEIALMADRMIRIEDGKVVNGNAEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1L6</accession>
    <name>R5V1L6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Sporulation transcriptional regulator SpoIIID</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01890</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000193">
        <property type="protein sequence ID" value="CCZ84165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01890"/>
    <dbReference type="EnsemblBacteria" id="CCZ84165">
        <property type="protein sequence ID" value="CCZ84165"/>
        <property type="gene ID" value="BN566_01890"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014208">
        <property type="entry name" value="Spore_III_D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018356">
        <property type="entry name" value="Tscrpt_reg_HTH_DeoR_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12116">
        <property type="entry name" value="SpoIIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00894">
        <property type="entry name" value="HTH_DEOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="111" mass="12797" checksum="63963D88336EAD84" modified="2013-07-24" version="1">MKQQPRERAIILGEYILEQQATVRAAAEWFGISKSTVHKDVSERLPDLDPHLYAGVKMILEKNKQERHIRGGLATRKKYALLAQQREQQKHAHFCHPAAEMPVRRDSCIQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1N3</accession>
    <name>R5V1N3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Endoglucanase</fullName>
            <ecNumber evidence="8">3.2.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN566_00362</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Endohydrolysis of (1-&gt;4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the glycosyl hydrolase 9 (cellulase E) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.4" evidence="8"/>
    <dbReference type="EMBL" id="CBAR010000238">
        <property type="protein sequence ID" value="CCZ84463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00362"/>
    <dbReference type="EnsemblBacteria" id="CCZ84463">
        <property type="protein sequence ID" value="CCZ84463"/>
        <property type="gene ID" value="BN566_00362"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110165">
        <property type="term" value="C:cellular anatomical entity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008810">
        <property type="term" value="F:cellulase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02850">
        <property type="entry name" value="E_set_Cellulase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004197">
        <property type="entry name" value="Cellulase_Ig-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003305">
        <property type="entry name" value="CenC_carb-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001701">
        <property type="entry name" value="Glyco_hydro_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033126">
        <property type="entry name" value="Glyco_hydro_9_Asp/Glu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02018">
        <property type="entry name" value="CBM_4_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02927">
        <property type="entry name" value="CelD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00759">
        <property type="entry name" value="Glyco_hydro_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="SSF48208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="SSF81296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00698">
        <property type="entry name" value="GH9_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 8" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3 8" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="5 7 8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 7 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 7 8" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005145533" description="Endoglucanase" evidence="8">
        <location>
            <begin position="36"/>
            <end position="1029"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="10">
        <location>
            <begin position="951"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="904"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="873"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="882"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ84463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="1029" mass="112069" checksum="E10A8BABD3CF3792" modified="2013-07-24" version="1">MLKNKFGKKAVAAVMAAAMTVSATAMGLSSITASAAVTGFEEFGAGTFNDGVGLPWHICESATGTMKFEVANGCYNILITNPGGLSNNGEGRWDCQFRHRGLTIEAGHTYRYTYSIWSNKDAKIYCKLGDITNDDLENWHQNGDKLQMDYDESLDDQQLTEKLKSASKTGEKVDFGMGWDSWKNQPTVTANKWTTYAWEFTAEKDSKGTAEMTFHLGGTSAYNDFICCEAGTLLKFDNLALVDMTDDKSNYNAEAAYQPTGVEVNQVGYYPLLEKKATLILDGPDTTAKDFQVKDSSGAVVYEGKTDASRGNQICDGSETYNQIIDFSDFQTEGTGYTITCDGKTSLPFDIGNNIYDGMTTNAMNYFYQNRSGVNIDAAYITSQGENSDKSKLARKAGHNPDTAYIQNKWVYIIPDENSIEKNNGTIDVTGGWYDAGDHGKYVVNGGVSMWTLLNLYESDLMAGDASKWADGSGTVVVPENGNSMPDILDEVKIEADFFKKMQRNDGMVYHKIHDYKWTALCVAPADDELTRIVKPVTYAATLNFAAAMAQYARLAKDYDKDASGYLADAEKAYKAAKASYKPFSNDWGTDQYADLESLYAPIAQNKGGGPYGDTDVEDEFYWAACELYIATGDASYKTDLEGYSAGAGAYGVDTALYGGENNGTRSSFTWGTLASLGTFSLCVNAKDMQEKGLLSAEEVSTIQKNVKQAADYFIDLENASDFGIPYVGHDYNADVWSVADYEAGNGVDGVVSKELKNGYEWGSNSMVMNNAITMALAYDIDHEAKYINGVSTAMDYILGRNVIEQSYVTGYGEHCLKYPHHRWWSGQLDAERFPYAPDGVLSGGPNSQMQDPMIQGAGYKAGSLAPMACYLDNVEAWSVNECTINWNSPLVWIASFLEDEAPNVNNETTKPSTTTTTDSDTTTTTTTTVATTTASGETTTAGSTTTVDPNADNIGDVNLDGIVDIADAVTLNKYLAGVVQLSDQALRNANCDQSPTDIDNVGDKDTTALVRFVLNMEGYQDLPFIAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1Q0</accession>
    <name>R5V1Q0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Amidohydrolase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01935</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01935"/>
    <dbReference type="EnsemblBacteria" id="CCZ84210">
        <property type="protein sequence ID" value="CCZ84210"/>
        <property type="gene ID" value="BN566_01935"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032465">
        <property type="entry name" value="ACMSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21240">
        <property type="entry name" value="PTHR21240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04909">
        <property type="entry name" value="Amidohydro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="3">
        <location>
            <begin position="4"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="273" mass="30687" checksum="C8A2CE08E9F96C3A" modified="2013-07-24" version="1">MPVIDFHTHVFPEAIAEKTISMLAQKSNSQPFGNGTFSSLLSNMQTSGVTHSVTLPVVTRPKQFDSILKYAIACNEHPQIFAFGGIHPDDPTPEEHLLQIKQAGLYGIKLHPDYQQTYIDDPKYIRILRICVTLQLPVVIHAGIDDGFPETVHCTPDRTLHMLEQVYGDTVPEQPNIVLAHGGANRMHEEVLEKLCGKPVYFDISFILSYIQPELLLQIIRKHGTDRILFATDFPWSDPAADLTAVQNLPLTDAEKEQILWKNAQTLLHLPQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1W0</accession>
    <name>R5V1W0_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribonuclease R</fullName>
            <shortName evidence="5">RNase R</shortName>
            <ecNumber evidence="5">3.1.13.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rnr</name>
        <name evidence="7" type="ORF">BN566_00029</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">3'-5' exoribonuclease that releases 5'-nucleoside monophosphates and is involved in maturation of structured RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.13.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the RNR ribonuclease family. RNase R subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.1" evidence="5"/>
    <dbReference type="EMBL" id="CBAR010000208">
        <property type="protein sequence ID" value="CCZ84290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00029"/>
    <dbReference type="EnsemblBacteria" id="CCZ84290">
        <property type="protein sequence ID" value="CCZ84290"/>
        <property type="gene ID" value="BN566_00029"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008859">
        <property type="term" value="F:exoribonuclease II activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01895">
        <property type="entry name" value="RNase_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013223">
        <property type="entry name" value="RNase_B_OB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001900">
        <property type="entry name" value="RNase_II/R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022966">
        <property type="entry name" value="RNase_II/R_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004476">
        <property type="entry name" value="RNase_II/RNase_R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011805">
        <property type="entry name" value="RNase_R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08206">
        <property type="entry name" value="OB_RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00773">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00955">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00358">
        <property type="entry name" value="3_prime_RNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02063">
        <property type="entry name" value="RNase_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01175">
        <property type="entry name" value="RIBONUCLEASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="S1 motif" evidence="6">
        <location>
            <begin position="638"/>
            <end position="718"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ84290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="721" mass="80182" checksum="D9E1C679959099E1" modified="2013-07-24" version="1">MAKTPSAPPKRDAAEILEAYQTKCKLFLADAGIHGLEETLLAAKCRTKKNGSALFQEALESLRKAGLIYKQRRKYYWCASLHCKTGTVVRLSRTFGFVRPDDPEAESDWFIPGKFLLGALPQDRVLMRSIPSRSGKPEGEVLDILEQASARLTGIIVYEDGKPFLLPDTMSKTPIRLLPNREVSYQEHDKVLAEIVSRGTRHAEHKAKVIYSFGNADCAAVCAQAQLTLENISPIFPEEVEKEAAALASQPIPKEAYQNRLDLRDACIFTIDSAESKDLDDAVSVERIPNGYRLGVHIADVSHYVKPHSALDKEALERGTSLYYADQVIPMLPKALSNGICSLNPQEDRLTFSAIMELDNQGVLRSYTFRKSIIRSKVKGVYKEINALLDGTADAALQEKYAEVLDMLPLLNELCDKRLCLRKQRGAPEIETPESKILLNADGICVDVQPRTRGKSECIIEEMMLLANESAARVAKEHALPFVYRVHDAPSSEKIEALQDGLLRMGAEVPVLTNVQPRNLAEILEKAAGTPAAPVIHGMVLRSMAKAAYETEPIGHFGLALSDYAHFTSPIRRYPDLAIHRILSEWCSTHSAEAVQKRFTKFAAKAAEQSSQRELLTIRVSRDCEACYFAEYMRQHLGETMNGLITGVTDFGIYVMLPNTVEGMISIRDLGEETVWTNDHNVRLVQENGDRVYQLGDTISVRCTKTDISSGNIDFVLAETE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1W5</accession>
    <name>R5V1W5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Fibronectin type III domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00034</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000208">
        <property type="protein sequence ID" value="CCZ84295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00034"/>
    <dbReference type="EnsemblBacteria" id="CCZ84295">
        <property type="protein sequence ID" value="CCZ84295"/>
        <property type="gene ID" value="BN566_00034"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026906">
        <property type="entry name" value="LRR_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13306">
        <property type="entry name" value="LRR_5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004406662" evidence="3">
        <location>
            <begin position="29"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="444"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="475" mass="51203" checksum="830F3A78D834E3FA" modified="2013-07-24" version="1">MMTRWKQTACGILSGMLLLSGLAVPVWAEDATDPVTNEGDWVVDDITLDMQDDGTAWVTNCFQSATSVDVPAEVDGVTISGIQEGAFSQCFLLESLTLPDTITTIQDSAFYGCSVLKKLEIPDSVTSMGKGTMEACIALTDVKLPSTLNTLPTSTFYGCTALETVSFPEAMNTIGSEAFYQCTALKDVDLSQIATIETYAFEGCSALTDITIPETCTTLGDFVFDGCSALKEIQVADSNPNYQDIDGVLFSKDGSTLIKYPDDSDLVDYAVPDGCTRLEDWSFTGAKSLKSIDIQNIQELGEDVFFYCQSLTDITIPDSITEIPNNTFTYCTELTSITLPKQLTSIGDFAFYTCVRLENITIPDTVTKIGKQAFYNCLELHELTLPSGIKELGDGAFGLYAESDDTSPSVVKDFSVTYGKNSKIKKYVSQYKIPATETAGSNSWVVPVCIGGGVVVVAAIVVILVVRKKRKGGAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1X8</accession>
    <name>R5V1X8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">30S ribosomal protein S5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rpsE</name>
        <name evidence="11" type="ORF">BN566_00074</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Located at the back of the 30S subunit body where it stabilizes the conformation of the head with respect to the body.</text>
    </comment>
    <comment type="function">
        <text evidence="7">With S4 and S12 plays an important role in translational accuracy.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Part of the 30S ribosomal subunit. Contacts proteins S4 and S8.</text>
    </comment>
    <comment type="domain">
        <text evidence="7">The N-terminal domain interacts with the head of the 30S subunit; the C-terminal domain interacts with the body and contacts protein S4. The interaction surface between S4 and S5 is involved in control of translational fidelity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 9">Belongs to the universal ribosomal protein uS5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000241">
        <property type="protein sequence ID" value="CCZ84528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00074"/>
    <dbReference type="EnsemblBacteria" id="CCZ84528">
        <property type="protein sequence ID" value="CCZ84528"/>
        <property type="gene ID" value="BN566_00074"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01307_B">
        <property type="entry name" value="Ribosomal_S5_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000851">
        <property type="entry name" value="Ribosomal_S5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005712">
        <property type="entry name" value="Ribosomal_S5_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005324">
        <property type="entry name" value="Ribosomal_S5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013810">
        <property type="entry name" value="Ribosomal_S5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018192">
        <property type="entry name" value="Ribosomal_S5_N_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13718">
        <property type="entry name" value="PTHR13718"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00333">
        <property type="entry name" value="Ribosomal_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03719">
        <property type="entry name" value="Ribosomal_S5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01021">
        <property type="entry name" value="rpsE_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00585">
        <property type="entry name" value="RIBOSOMAL_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50881">
        <property type="entry name" value="S5_DSRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 7 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 7" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="S5 DRBM" evidence="10">
        <location>
            <begin position="11"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ84528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="166" mass="17067" checksum="D9576E4030EBD910" modified="2013-07-24" version="1">MAKIDPNSLELTEKVVSINRVSKTVKGGRIMKFAALVVVGDGNGTIGFGLGKSGEVPDAIRKGIEDAKKNLIKIPLKGTTIPHEIVGKFGAGSVLMKPAAPGTGVIAGGPVRAVIEVAGIKDIRTKSLRSNNPCNVVRATINGLASCATAAEVAEMRGKSVKEILG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1Z8</accession>
    <name>R5V1Z8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00253</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000217">
        <property type="protein sequence ID" value="CCZ84340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00253"/>
    <dbReference type="EnsemblBacteria" id="CCZ84340">
        <property type="protein sequence ID" value="CCZ84340"/>
        <property type="gene ID" value="BN566_00253"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025584">
        <property type="entry name" value="Cthe_2159"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14262">
        <property type="entry name" value="Cthe_2159"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="466" mass="49668" checksum="45E2F2CCA7803C7F" modified="2013-07-24" version="1">MYPKKGWILAAVFLVLCSVGCTGQTGETHRLYVQTAGSESQIAETLPTAEQAEWMDADSNVVSTKAVKSKKATKTTTKKKKSSKKKTTTKQKKETTAKVTTKKAAKTTKPKTTKASKTTTAPRIAATMPRENLFRTTTVRNDTANASNRTTKTSSKAESPASVKMTTLKTNVVIRTQPPVPVTIAPVETNPTQPEQQTDKYLSLSDGEMQGAGIVVSGNQIFLQAAGIYHLSGNWNGSIYVQAGTTDTVELRLEGASICGVNAPAIQIQQAGMVTITTADHTENTILAEQAGSVIDSSSALQLQGTGALQIQNESGNGIACTDAITITEGMFSIAAKDTAILSGRDSKIVDGTVIANGGQSGIRCDGRFSVEGGTLLATGTNANIADSIQSTAQLNWQETITSGHTVSFQINGIEVVKTMLQYDAETFFLTCPDLTIGNHVDFLIDGINVKTFEQQEQDARWNEKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5V296</accession>
    <name>R5V296_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Peptidase family M50</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00347</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000228">
        <property type="protein sequence ID" value="CCZ84440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00347"/>
    <dbReference type="EnsemblBacteria" id="CCZ84440">
        <property type="protein sequence ID" value="CCZ84440"/>
        <property type="gene ID" value="BN566_00347"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="176" mass="19144" checksum="17F46924B3A895BE" modified="2013-07-24" version="1">MVRITVPFLAILAWLFACSDSALRQFAFGACLLHESGHLVMMGLLRQPVRQITCYGVGIQIQAESCLYPLWQDALVLLAGPFCNLLFAGVLWLCGVREAAVAVQLGTGLLNLLPFQQLDGGAFLRLWLPEQGEWVLRGVCVLIAVGGLCWGGVAGITNITYYCFLGSLLLTELLTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5V2A3</accession>
    <name>R5V2A3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Integral membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00044</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000230">
        <property type="protein sequence ID" value="CCZ84445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84445">
        <property type="protein sequence ID" value="CCZ84445"/>
        <property type="gene ID" value="BN566_00044"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026870">
        <property type="entry name" value="Zinc_ribbon_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13240">
        <property type="entry name" value="zinc_ribbon_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="182"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="220"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="zinc_ribbon_2" evidence="3">
        <location>
            <begin position="3"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="270" mass="28796" checksum="F7CD6F4810725577" modified="2013-07-24" version="1">MPFCGNCGHPLSDTDLTCPNCGEIRPNAIPKPDSINQEPTAPTPDTPTEPNLNPEQSNSNTTAQSADTAVSDNYDHVAAYQNNAQADTPVANDIPKEVPPQPDTSATQNTTTETNAAPDFSAYDIRNNIPNGNTANQQQPNFSGQFQQQSTAGQSPYTYNDPYSTPNTPLTNNNNGMGIASLILGICSIAICCCYGVGAIPAIIGLILGILQNKKNANGIATAGIVLGIIGILLNVVWLIYMIIFLSEDGLLDFVNEFSSNYEYYENSTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>R5V2C6</accession>
    <name>R5V2C6_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ferrous iron transport protein B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BN566_00551</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Probable transporter of a GTP-driven Fe(2+) uptake system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. FeoB GTPase (TC 9.A.8) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000270">
        <property type="protein sequence ID" value="CCZ84698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00551"/>
    <dbReference type="EnsemblBacteria" id="CCZ84698">
        <property type="protein sequence ID" value="CCZ84698"/>
        <property type="gene ID" value="BN566_00551"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015093">
        <property type="term" value="F:ferrous iron transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055072">
        <property type="term" value="P:iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01879">
        <property type="entry name" value="FeoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003373">
        <property type="entry name" value="Fe2_transport_prot-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011640">
        <property type="entry name" value="Fe2_transport_prot_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041069">
        <property type="entry name" value="FeoB_Cyto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030389">
        <property type="entry name" value="G_FEOB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011642">
        <property type="entry name" value="Gate_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07664">
        <property type="entry name" value="FeoB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17910">
        <property type="entry name" value="FeoB_Cyto"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02421">
        <property type="entry name" value="FeoB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07670">
        <property type="entry name" value="Gate"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00437">
        <property type="entry name" value="feoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51711">
        <property type="entry name" value="G_FEOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0410">Iron transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="280"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="345"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="436"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="459"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="517"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="556"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="583"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="619"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="653"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="domain" description="FeoB-type G" evidence="11">
        <location>
            <begin position="11"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CCZ84698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="677" mass="73557" checksum="61A7FFD8295C1022" modified="2013-07-24" version="1">MYPPPESTIESYTVALAGNPNVGKSTVFNALTGMHQHTGNWAGKTVSTAAGICTISGVSIQLIDLPGTYSLRAQSAEEAAARDFLCFSRPDAVLVVCDASALERNLILLLQIQELGLPVFLCINLLDEAEQRGIALDFPLLSEQLHLPVVGVAARSGRGLPELQEQLLSFLRQPFVSLHAVPYPESIETAIATITEQLPQLDFPPTPRWLALRFLEQDATLQMERLLPEEPISELEIPVLPKAADSIIAAVVQQAESIANTVTTIPESIRKREHRFDRFVLGKYTGIPLLCLLLLLILWITVVGANVPSGLLQTGFAKLRTVLDSWLSGTPEWVQGLLLDGMYQVLTWVIAVMLPPMAIFFPLFTLLEDFGYLPRVAYQLDPCFRCAGTCGKQSLTMCMGLGCNAAGVTGCRIIDSPRERLIAMLTNVCIPCNGRFPAMITILTLFFAASLGWGSSLTAAIGILCLLLLGTGMTFLLSFLLSHTLLKGIPSSFTLELPPYRKPQIGKVLVRSVCDRTLFVLGRACIVAAPAGMIIWMLANGTLSNGQSILCQLAEWLQPVGAIFGLDGAILLAFLLGFPANEIVLPIVLMIYAASGTLTEQTDLSQIQTMLQANGWTMQTAVCMLLLMLFHFPCSTTCLTIRKETHSWKWTGVAMLLPTLCGLGLCALVHGVWSMFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>R5V2N3</accession>
    <name>R5V2N3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5 7">50S ribosomal protein L15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rplO</name>
        <name evidence="10" type="ORF">BN566_00076</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the universal ribosomal protein uL15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000241">
        <property type="protein sequence ID" value="CCZ84530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00076"/>
    <dbReference type="EnsemblBacteria" id="CCZ84530">
        <property type="protein sequence ID" value="CCZ84530"/>
        <property type="gene ID" value="BN566_00076"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01341">
        <property type="entry name" value="Ribosomal_L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="L18e/L15P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030878">
        <property type="entry name" value="Ribosomal_L15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005749">
        <property type="entry name" value="Ribosomal_L15_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001196">
        <property type="entry name" value="Ribosomal_L15_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_L18e/L15P"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12934">
        <property type="entry name" value="PTHR12934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00828">
        <property type="entry name" value="Ribosomal_L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="SSF52080"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01071">
        <property type="entry name" value="rplO_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00475">
        <property type="entry name" value="RIBOSOMAL_L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Ribosomal_L18e/L15P" evidence="9">
        <location>
            <begin position="27"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ84530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="146" mass="15189" checksum="675CD9D78D00E454" modified="2013-07-24" version="1">MKLHELSPAAGSVKDVKRVGRGHGSGNGKTAGKGHKGQNARSGGGVRIGFEGGQMPIARRIPKRGFKNIFGTDYAIVNVADLNQFAEGTVVDAELLKATGLVKKVYAGVKVLGNGELTTKLTVKAAKFSKSAVEKIEKAGGKAEVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5V2S2</accession>
    <name>R5V2S2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00431</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000247">
        <property type="protein sequence ID" value="CCZ84570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00431"/>
    <dbReference type="EnsemblBacteria" id="CCZ84570">
        <property type="protein sequence ID" value="CCZ84570"/>
        <property type="gene ID" value="BN566_00431"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="2">
        <location>
            <begin position="66"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="207"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="386" mass="43973" checksum="EED0443E7C391BA2" modified="2013-07-24" version="1">MRIALDALPLVSSRMTGIGYCEAGQVQAMMMLHPEEQYVLQFFDKGDGAEKRKRLYYYEQQRNVSVRQGHFSGYLYRLMTTAIPLPYRAFFGKDVDITHFFNYIIPPGVAGKRIVTVHDMVYKTFPETVRGRTRFMLESGLQRSLHRADLIVTDSEFSKTEIIRYFPATEERIRVVPCGVDKLRFSPMPPAAAPAIEELKRRMHLQANYFLYLGTLEPRKNLDRLIRAYALLKQQHPDAPQLVLAGGYGWRNNHIYQLVQTLHLEDDVLFTEYVPEEDVQPLLCGAMAFVFPSVYEGFGMPPLEAMACGTPVLTTRAASLPEVVGDAAVIVDPHHTEDIAQGLEKLFLDAALRERLQKEGLRRAAMFSWENAAAALYDVYQEALTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5V300</accession>
    <name>R5V300_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Chaperone protein DnaK 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00508</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000266">
        <property type="protein sequence ID" value="CCZ84655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00508"/>
    <dbReference type="EnsemblBacteria" id="CCZ84655">
        <property type="protein sequence ID" value="CCZ84655"/>
        <property type="gene ID" value="BN566_00508"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="PTHR19375"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="SSF100920"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="365" mass="41452" checksum="1A65EC845D9026DB" modified="2013-07-24" version="1">MQKKSYKIAINWIQEPTLGTKLKVAAQIYGKTFVIGEVRSPFQDGVQAMQKKDRHEILYLLSQAKEMAEKILSDDQTEAVVKEVYLVLPDSFLKQEKDTIEMICEQLGMFICYTIYHSFAAIQYLSTLGELPEEQAVLSVQEKDCYFAVTESSDGVCQVLAKNHYPQFKGMSFFDSDDLFSIFSIIDRTISEANKEIPTTIDDFDRLIITGDIASELSFQQQLSARYGKALTIVSEDEIAMGVLCEDRTDCLLVLNCLSHSISVETAGSTLQKVIEKNTTIPTKRSFLLETADSQQRAMDIEIFMGEAYNTKDNKKIGYYRFEPVLPQSEIEIIFDVDELESLTVTIQDLSNSSTREIIRNMQIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>R5V346</accession>
    <name>R5V346_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH marR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00558</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000273">
        <property type="protein sequence ID" value="CCZ84705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00558"/>
    <dbReference type="EnsemblBacteria" id="CCZ84705">
        <property type="protein sequence ID" value="CCZ84705"/>
        <property type="gene ID" value="BN566_00558"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023187">
        <property type="entry name" value="Tscrpt_reg_MarR-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01117">
        <property type="entry name" value="HTH_MARR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="158" mass="18154" checksum="0F3EACDFD0D1CD4B" modified="2013-07-24" version="1">MNIHLSKQLLHWNSLYQQLNETYRQYATQKGVSETTLWLLYFLCEQGGSCEQRALCRSLLDPPQTVNSSLKKLEQQGLISLKLVAGSRKNKEVSLTEAGEQFAKTTVLPLMAAEQQSFSDLSEREQLQMLTLMQKHQDLLRMELERAACIDPEEEQHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5V3V6</accession>
    <name>R5V3V6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">FAD dependent oxidoreductase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00589</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000008">
        <property type="protein sequence ID" value="CCZ82784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00589"/>
    <dbReference type="EnsemblBacteria" id="CCZ82784">
        <property type="protein sequence ID" value="CCZ82784"/>
        <property type="gene ID" value="BN566_00589"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DAO" evidence="1">
        <location>
            <begin position="8"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="377" mass="40881" checksum="137354FF1FC83C76" modified="2013-07-24" version="1">MLEKREADVIIIGGGIMGCATAYRLAKQGVDVLVLEKKEIGNGGSCRNAGGVRQSARDPKELPLAMYAVANIWPHLSEELGVDVEYHQGGNLRLGLTEKDIKVLENHTAKSLKLGLDVKMISGDEAREICPYMSKEVVAASWCPTDGHANPLKATLGFYRKALELGVTFITGVKVEALEKVKGRIRRVLTDDGVYEADQILLCAGYESRKIANTVGVDAPVERQFNEVLVTEAQPKMFDIMIGVAGGEFYGHQSEHGSFVLGGNSGLQAFTSNNDNFVTNSLTAPCISRGIIKYFPILDKVKVVRTWSGWYDQCIDVLPVIDNVKEVPGLTVAFGFSGHGFGISPAVSIALSELILNGKSTTIDISQLNYDRFKAKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5V3Y1</accession>
    <name>R5V3Y1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CotJC protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00624</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the manganese catalase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000011">
        <property type="protein sequence ID" value="CCZ82819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00624"/>
    <dbReference type="EnsemblBacteria" id="CCZ82819">
        <property type="protein sequence ID" value="CCZ82819"/>
        <property type="gene ID" value="BN566_00624"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01051">
        <property type="entry name" value="Mn_catalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007760">
        <property type="entry name" value="Mn_catalase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039377">
        <property type="entry name" value="Mn_catalase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05067">
        <property type="entry name" value="Mn_catalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="209" mass="23916" checksum="AC71756368FDB03E" modified="2013-07-24" version="1">MWTYEKKLQYPVNIKTPNPRLAKFIISQLGGPDGELAASLRYMHQRYGMKNNQVKGILTDIATEELAHVEMVSAILYQLTRDMTPEQILQDGYAEYFVDHTAGIYPQGASGMPFTAAYFQSKGDPITDLTEDLAAEQKARSTYENILRLCDDPDVREPIKFLRQREIVHFQRFGEAMRMVQDELNSKNFYCCNPAFDKGCGKQQKRSGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5V426</accession>
    <name>R5V426_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Threonine-phosphate decarboxylase</fullName>
            <ecNumber evidence="5">4.1.1.81</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">L-threonine-O-3-phosphate decarboxylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN566_00671</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Decarboxylates L-threonine-O-3-phosphate to yield (R)-1-amino-2-propanol O-2-phosphate, the precursor for the linkage between the nucleotide loop and the corrin ring in cobalamin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H(+) + O-phospho-L-threonine = (R)-1-aminopropan-2-yl phosphate + CO2</text>
            <dbReference type="Rhea" id="RHEA:11492"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58563"/>
            <dbReference type="ChEBI" id="CHEBI:58675"/>
            <dbReference type="EC" id="4.1.1.81"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.81" evidence="5"/>
    <dbReference type="EMBL" id="CBAR010000019">
        <property type="protein sequence ID" value="CCZ82869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00671"/>
    <dbReference type="EnsemblBacteria" id="CCZ82869">
        <property type="protein sequence ID" value="CCZ82869"/>
        <property type="gene ID" value="BN566_00671"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048472">
        <property type="term" value="F:threonine-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005860">
        <property type="entry name" value="CobD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01140">
        <property type="entry name" value="L_thr_O3P_dcar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="10">
        <location>
            <begin position="19"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ82869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="350" mass="38762" checksum="C83B41C23E55E749" modified="2013-07-24" version="1">MTSMQHGGNIYTEQTNAPDLLDFSANINPFGVPESVKQAISAAIPTLVHYPDPQQTALTQAIATYHALQPEQIVCGNGGADVLFRVLQCLHPKRALLPVPSFLEYETALQEIGCSVIHWQMSPNLTITDAILPELQTGQYDCLMLCNPNNPTGTLLQKSQLLPILDCCLQNGIRVILDECFCDMTSEAQQVSLLASCTAYPNVFFLKSLTKRFAIPGLRLGYGVCADVALIKKVRQTGQPWSVNTLAAAAGIAALQDTAYQQQFQTFLEQEQPAFYHALQAIPTLQVWKPAANFVFFRAACTDLQERLLHRHILIRHCENYVGLDATYYRTAIRSHAENQILLQALKACL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5V445</accession>
    <name>R5V445_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">5-nitroimidazole antibiotic resistance protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00691</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000022">
        <property type="protein sequence ID" value="CCZ82889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00691"/>
    <dbReference type="EnsemblBacteria" id="CCZ82889">
        <property type="protein sequence ID" value="CCZ82889"/>
        <property type="gene ID" value="BN566_00691"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024747">
        <property type="entry name" value="Pyridox_Oxase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12900">
        <property type="entry name" value="Pyridox_ox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ82889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="161" mass="18484" checksum="5C841A703178FC66" modified="2013-07-24" version="1">MRRSEREVTDCNEIRQILERAEVLRLAMHDEPYPYIVPVNFGFTMQDDQLVLFFHGAKVGKKCDLLQKNPHVSFEVEGKQQLIPPNGAASCTAGFAYESVIGQGIAELAPEEKKEDLLKKLLEHYRIPFQSFSQAHLERTAVYQIIVKQYTAKRRPIPQGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5V4M6</accession>
    <name>R5V4M6_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal RNA small subunit methyltransferase I</fullName>
            <ecNumber evidence="6">2.1.1.198</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">16S rRNA 2'-O-ribose C1402 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">rRNA (cytidine-2'-O-)-methyltransferase RsmI</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rsmI</name>
        <name evidence="8" type="ORF">BN566_00832</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the 2'-O-methylation of the ribose of cytidine 1402 (C1402) in 16S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>cytidine(1402) in 16S rRNA + S-adenosyl-L-methionine = 2'-O-methylcytidine(1402) in 16S rRNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42924"/>
            <dbReference type="Rhea" id="RHEA-COMP:10285"/>
            <dbReference type="Rhea" id="RHEA-COMP:10286"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74495"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.198"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the methyltransferase superfamily. RsmI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.198" evidence="6"/>
    <dbReference type="EMBL" id="CBAR010000040">
        <property type="protein sequence ID" value="CCZ83039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00832"/>
    <dbReference type="EnsemblBacteria" id="CCZ83039">
        <property type="protein sequence ID" value="CCZ83039"/>
        <property type="gene ID" value="BN566_00832"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070677">
        <property type="term" value="F:rRNA (cytosine-2'-O-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000453">
        <property type="term" value="P:enzyme-directed rRNA 2'-O-methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11648">
        <property type="entry name" value="RsmI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01877">
        <property type="entry name" value="16SrRNA_methyltr_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008189">
        <property type="entry name" value="rRNA_ssu_MeTfrase_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46111">
        <property type="entry name" value="PTHR46111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005917">
        <property type="entry name" value="MTase_YraL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="SSF53790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00096">
        <property type="entry name" value="TIGR00096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TP_methylase" evidence="7">
        <location>
            <begin position="4"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="281" mass="31356" checksum="646DC0699902E6DE" modified="2013-07-24" version="1">MSGTLYVIGTPIGNLEDMTLRQLRMLQEVDFIAAEDTRVTRKLLSHYDIHTPMVSYHDHSGQLVTQQLLQRIAAGETCGIVTDAGMPCISDPGEILVRCCKEQGIPVTAVPGPSAAITALAISGQETGRFTFEGFLSVTKKQRMEHLHSLQTEHRTMIFYEAPHKLLRTLTDLVACFGGERPVSICRELTKLHEEVWKTTLAEALHRYEQQPPKGEFVLIVAGMPESTDETTLSMEDALQQVQERVAQGERLTDACKAVAAETGYRKQDLYQQAIQERESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>R5V4S1</accession>
    <name>R5V4S1_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HTH araC/xylS-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00887</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000055">
        <property type="protein sequence ID" value="CCZ83099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00887"/>
    <dbReference type="EnsemblBacteria" id="CCZ83099">
        <property type="protein sequence ID" value="CCZ83099"/>
        <property type="gene ID" value="BN566_00887"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018062">
        <property type="entry name" value="HTH_AraC-typ_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020449">
        <property type="entry name" value="Tscrpt_reg_HTH_AraC-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00032">
        <property type="entry name" value="HTHARAC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00041">
        <property type="entry name" value="HTH_ARAC_FAMILY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="2">
        <location>
            <begin position="243"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="351" mass="40642" checksum="67E0C66CB03304D0" modified="2013-07-24" version="1">MNDNREIGYCLDSNHFCYGFITSSNRKIIVGPVSELEKTQQELRHIAFKLNYQNTEFLIAQMQAICIMHPDTLLQSLIFLNFCINKTMMEISDVRIKKEQQIGITTDFKEHTSFEKKDAFLTRSRSYVIDQGIVRKIKAGDVEGLKQGANQVPSANPRNFAPHLLRHTKNFFIRLLAICCFAAMEAGVNSTEIAELEELYILKCESLEDVERIKNLQYHMILDLAERVQALHKRNPNHSQLVKNITNYIKENLTERITVSEIAEYLSKSRGYVTTEFKKITGMNLSDYISELKINEAKELIRYTDRSFIDISSYLGFSTQSHFIKVFKKVTGKTPRAYRELANLDEEERLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5V560</accession>
    <name>R5V560_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MATE efflux family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01045</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83264">
        <property type="protein sequence ID" value="CCZ83264"/>
        <property type="gene ID" value="BN566_01045"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006603">
        <property type="entry name" value="DinF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="241"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="270"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="318"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="361"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="390"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="424"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="460" mass="50005" checksum="7F6AEB3419A115AD" modified="2013-07-24" version="1">MHTAKKQYEMDMCSGSILKKMLLFTLPLMLSSILQLLFNAADIVVVGKFAGDNALAAVGSNTALINLLTNLFVGFSIGANVTAARHYGAKCEKDLEETVHTSIAMSFASGLLLMIVGLIGAKQFLIWMQTPEEVLPLATLYLHIYFIGMIPNMVYNFGSALLRSVGDTRRPLYYLTFAGCVNVVLNLLTVIVLHLDVAGVAIATVISETISAVLVLRCMCKEQGAMHLQWSKLRIHRGKMLQIMKIGIPAGFQGVVFALSNVVIQSSVNLFGNIVVAGNSAAANIEGFVYMAMNAFYQATISFTSQNYGAGKYNRINRILLTGQGCVIVVGVVLGNLAVLFGHQLLHIYSSNPDVIAAGMVRLHIISATYALCGIMDVMVGALRGLNYSVLPMVVSLVGACGLRLVWIATVFQMPEFHTVQIVYLSYPITWIITLSTHILCYCIIKRRLTKKLALQPAAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>R5V5B1</accession>
    <name>R5V5B1_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">1,4-alpha-glucan branching enzyme GlgB</fullName>
            <ecNumber evidence="12">2.4.1.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Alpha-(1-&gt;4)-glucan branching enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Glycogen branching enzyme</fullName>
            <shortName evidence="12">BE</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">glgB</name>
        <name evidence="16" type="ORF">BN566_01100</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 12">Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>Transfers a segment of a (1-&gt;4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.</text>
            <dbReference type="EC" id="2.4.1.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">Glycan biosynthesis; glycogen biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.18" evidence="12"/>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01100"/>
    <dbReference type="EnsemblBacteria" id="CCZ83319">
        <property type="protein sequence ID" value="CCZ83319"/>
        <property type="gene ID" value="BN566_01100"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00164"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003844">
        <property type="term" value="F:1,4-alpha-glucan branching enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102752">
        <property type="term" value="F:1,4-alpha-glucan branching enzyme activity (using a glucosylated glycogenin as primer for glycogen synthesis)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043169">
        <property type="term" value="F:cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02855">
        <property type="entry name" value="E_set_GBE_prok_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00685">
        <property type="entry name" value="GlgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006048">
        <property type="entry name" value="A-amylase/branching_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037439">
        <property type="entry name" value="Branching_enzy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006407">
        <property type="entry name" value="GlgB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044143">
        <property type="entry name" value="GlgB_N_E_set_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="Glyco_hydro_13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004193">
        <property type="entry name" value="Glyco_hydro_13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43651">
        <property type="entry name" value="PTHR43651"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02806">
        <property type="entry name" value="Alpha-amylase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02922">
        <property type="entry name" value="CBM_48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000463">
        <property type="entry name" value="GlgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="SSF81296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01515">
        <property type="entry name" value="branching_enzym"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="9 12" id="KW-0320">Glycogen biosynthesis</keyword>
    <keyword evidence="5 12" id="KW-0321">Glycogen metabolism</keyword>
    <keyword evidence="11" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aamy" evidence="15">
        <location>
            <begin position="153"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="643"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="12 13">
        <location>
            <position position="310"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="12 13">
        <location>
            <position position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000463-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CCZ83319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="663" mass="77134" checksum="3948B84FFE2925BB" modified="2013-07-24" version="1">MLATTKANENDFPLYLFHQGKNAEAGKFFGAHAFKRGRGSYHRFRVWAPTAKSVSVIGEFNQWDRTQNPMEKISDTVWEVVIPKLKQFDLYKYSIETQNGQILEKSDPYAVHFETRPGTASKLFESSYQWEDAAWMQQRKKWIPYKEPMNIYEVHLGSWKHKEGEQFPSYLKFATEIIPYLKKMSYTHVEFMPLTEYPFDGSWGYQVTGYFAPTSRHGTPDDFRKMVDLFHQANIGVILDWVPAHFPKDAAGLCEFDGSYCYEYTDPLKMEHKSWGTRVFDYGKGEVCSFLISSACSWLEDYHVDGLRVDAVASMLYLDYDRQEWRPNVYGGHENLEAVDFLRRLNETVFSRNPDVLMIAEESTAWPLVTKPTDIGGLGFNFKWNMGWMNDMLSYVSLDPIYRSFNHDKLTFSFFYCFSENYILPISHDEIVYGKCSMLKKMSGNETDQYATYRSFLAYMMAHPGKKLLFMGQEFAQVNEWNYQTQLDWYLLDQPEHQKMLLFSEALNKFYLEHAPLWQNDDSWKGFSWISNDDYQQSVIAFRRIDDDGNEVMVVCNFCPVLRNDYCIGVPFAGKYELIFNTDDAVFGGSGVSEKTFRTIAEPMHGFEQHISMTLAPLSVVYLKYVPTKKRLTRKGTTTKIHARRATRSIASSAETAEKKQNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5V5B8</accession>
    <name>R5V5B8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01110</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000087">
        <property type="protein sequence ID" value="CCZ83329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83329">
        <property type="protein sequence ID" value="CCZ83329"/>
        <property type="gene ID" value="BN566_01110"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="87" mass="10222" checksum="525C23D3E8AE2428" modified="2013-07-24" version="1">MFYLTYNYTNARRSRMAIACVIKCKNTYGKQLRVRDIIEMHRPGLYQLLSRGDQCRVGRAISRLYNQGMLPHLNRGKKKGSTNPYYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5V5T1</accession>
    <name>R5V5T1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphoenolpyruvate-protein phosphotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01248</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000115">
        <property type="protein sequence ID" value="CCZ83479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01248"/>
    <dbReference type="EnsemblBacteria" id="CCZ83479">
        <property type="protein sequence ID" value="CCZ83479"/>
        <property type="gene ID" value="BN566_01248"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016772">
        <property type="term" value="F:transferase activity, transferring phosphorus-containing groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000121">
        <property type="entry name" value="PEP_util_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_Kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02896">
        <property type="entry name" value="PEP-utilizers_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="SSF51621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PEP-utilizers_C" evidence="1">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="92" mass="10428" checksum="8B3775CE547CECCE" modified="2013-07-24" version="1">MTQYTLACDRQNPDIESFCDTHHEAILRLIAWSAKNAHEHGAWIGICGELAADTSLTERFLRMGIDELSVSPTFVLKVRDAVRKVDLRTKEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5V6B3</accession>
    <name>R5V6B3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01400</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000148">
        <property type="protein sequence ID" value="CCZ83664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83664">
        <property type="protein sequence ID" value="CCZ83664"/>
        <property type="gene ID" value="BN566_01400"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="98" mass="11335" checksum="38C6D9020A451E98" modified="2013-07-24" version="1">MKHNLRELDEGQLQTMWSFLKLQPKNTCTKEDVRILKEHLDIIRQAMVQKTAGQRDTDPDTYVDFVEIGTYINFVVIEALQLRIYGGLDALEEVLPGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5V6Q1</accession>
    <name>R5V6Q1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Small-conductance mechanosensitive channel</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_01534</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000164">
        <property type="protein sequence ID" value="CCZ83799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01534"/>
    <dbReference type="EnsemblBacteria" id="CCZ83799">
        <property type="protein sequence ID" value="CCZ83799"/>
        <property type="gene ID" value="BN566_01534"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045275">
        <property type="entry name" value="MscS_archaea/bacteria_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011066">
        <property type="entry name" value="MscS_channel_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011014">
        <property type="entry name" value="MscS_channel_TM-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008910">
        <property type="entry name" value="TM_helix"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30221">
        <property type="entry name" value="PTHR30221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05552">
        <property type="entry name" value="TM_helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="SSF50182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82689">
        <property type="entry name" value="SSF82689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82861">
        <property type="entry name" value="SSF82861"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="92"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="310" mass="34032" checksum="30449C3EE622E76A" modified="2013-07-24" version="1">MQTTQLLTTTFETTSPLTETQTAPLENVVESMGEEVHHASRVLNAVLTPIKNAIPSLIMAIIFAFIGYALIKYLLRLVSHGLKRSNLDRIAVGFIRSVIQIILYAILGVIVLSILDVPMDSIVAVIASAGVAVALALKDSLSNLAGGFILLFSKPVKQGDTVQIDGTIGKVEQIDILYTKIVTADNTVAFIPNGKVTAAKIINYTEKETRRVELNLGISYESDLETARKIILETVAKHPLTLKDPVPDVVVKNQGSDAIELLLRVWCNSTDYWTVYYDMMEQTKQALDENGIEIPYHKVDVHVLESADEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>R5V730</accession>
    <name>R5V730_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 8">UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase</fullName>
            <ecNumber evidence="7 8">6.3.2.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">D-alanyl-D-alanine-adding enzyme</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">murF</name>
        <name evidence="12" type="ORF">BN566_01633</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 8">Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>ATP + D-alanyl-D-alanine + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate = ADP + H(+) + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine</text>
            <dbReference type="Rhea" id="RHEA:28374"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57822"/>
            <dbReference type="ChEBI" id="CHEBI:61386"/>
            <dbReference type="ChEBI" id="CHEBI:83905"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7 8">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the MurCDEF family. MurF subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.10" evidence="7 8"/>
    <dbReference type="EMBL" id="CBAR010000172">
        <property type="protein sequence ID" value="CCZ83904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01633"/>
    <dbReference type="EnsemblBacteria" id="CCZ83904">
        <property type="protein sequence ID" value="CCZ83904"/>
        <property type="gene ID" value="BN566_01633"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047480">
        <property type="term" value="F:UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008766">
        <property type="term" value="F:UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02019">
        <property type="entry name" value="MurF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004101">
        <property type="entry name" value="Mur_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000713">
        <property type="entry name" value="Mur_ligase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035911">
        <property type="entry name" value="MurE/MurF_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005863">
        <property type="entry name" value="UDP-N-AcMur_synth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01225">
        <property type="entry name" value="Mur_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02875">
        <property type="entry name" value="Mur_ligase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="SSF53244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63418">
        <property type="entry name" value="SSF63418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01143">
        <property type="entry name" value="murF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7 8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1 7 8" id="KW-0132">Cell division</keyword>
    <keyword evidence="3 7 8" id="KW-0133">Cell shape</keyword>
    <keyword evidence="6 7 8" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 7 8" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mur_ligase" evidence="9">
        <location>
            <begin position="26"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_M" evidence="11">
        <location>
            <begin position="103"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_C" evidence="10">
        <location>
            <begin position="319"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="105"/>
            <end position="111"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CCZ83904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="460" mass="49188" checksum="956C42ED62F032C4" modified="2013-07-24" version="1">MEKLQLSEIAAAVGSETPLEAAVNDICTDTRKLEPGCLFLALRGANFDGHQFVKKAIADGAVAAVTDQPIDGCPCIVVPDTGTALLQIAAYYRKKFSPILVGVTGSVGKTTTKEMIALVLSAAFETLKTQGNLNNEIGLPKTLLHLAPEHKAAVIEMGMSHFGEIHRLSCTTQPTIGVITNIGFSHIENLGSQEGILKAKLEILDGMQPDAPLILNADDPHLAPLAGTLDRPVYTYGLNAKDADVTAEDVQEGENATTFTIVTKDGRTFPAELPCVGLHNVMNALAAFCVGRICGIAPDVICAALKHYQPIPFRQNIEQRGPYTVIADCYNASPDSMRAALYVLRQMKGEGRRVAVLGDMLELGKLSPKLHSMVGDMASASHLDQLFCYGKASIDIAKTAAADGTSVFHTEDSTELCSAVRAYLRPGDVILFKASHGMHLETCIEEIFGQEEVAHAAVEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5V758</accession>
    <name>R5V758_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Carbohydrate binding module (Family 6)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01663</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000173">
        <property type="protein sequence ID" value="CCZ83934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01663"/>
    <dbReference type="EnsemblBacteria" id="CCZ83934">
        <property type="protein sequence ID" value="CCZ83934"/>
        <property type="gene ID" value="BN566_01663"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002105">
        <property type="entry name" value="Dockerin_1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00404">
        <property type="entry name" value="Dockerin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004406803" evidence="2">
        <location>
            <begin position="27"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="3">
        <location>
            <begin position="152"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="238"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="541" mass="59804" checksum="2E3535328C0FEAEF" modified="2013-07-24" version="1">MKRKSLQTALLAAVMGVVSMPHAVLAADAPSEAAAAVNVWIGYQLRDDAGQYLTVANGAAETGSQILSYAANGVAAYNIWYFTETGTGIYTLKSSLNEGEFYATASDRQTLELQLQQDGMQQQFTCTEVGMNRIHLQQNETDFGTYTLEPVTWLKPGDVNRDGVVNVLDLSLAKRLVLQGGGSDFCAVALADVDGNGTLDAADLAALQGFLMQRQTAFPAETVTLPENTIFPVVEPEQTTTTTSIATTTTAPEETTTTTTTTTDSKQTLTIADMPASYQFAADWIWTNRVEREQSTVRRNTLFDQIVAGNGELHYVVRWQSYKTVSLEQRKQFEKLVEDSINAWTDWLKDYEDWPYDHVTVKIVGWAVLDRNCLLDLQPDEVVYDSLISDYDSSGDTSNGYETIPDKLPSAPTDLSRMEHFMDTSYVYPGERFDMYLWATQGFPSIGGCGGDWGQRLSDDAYLNMLDGTGLHVLEHELGHGFGMTDFYGGEGELDGFPPGGFPGGSSLMMAGSSMQITDFDGWMLRYFWDKIHLEEGRFSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7T2</accession>
    <name>R5V7T2_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 8">UDP-glucose 4-epimerase</fullName>
            <ecNumber evidence="5 8">5.1.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN566_01894</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>UDP-alpha-D-glucose = UDP-alpha-D-galactose</text>
            <dbReference type="Rhea" id="RHEA:22168"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="EC" id="5.1.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 8">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 8">Carbohydrate metabolism; galactose metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 8">Belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.2" evidence="5 8"/>
    <dbReference type="EMBL" id="CBAR010000193">
        <property type="protein sequence ID" value="CCZ84169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01894"/>
    <dbReference type="EnsemblBacteria" id="CCZ84169">
        <property type="protein sequence ID" value="CCZ84169"/>
        <property type="gene ID" value="BN566_01894"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00214"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003978">
        <property type="term" value="F:UDP-glucose 4-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006012">
        <property type="term" value="P:galactose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd05247">
        <property type="entry name" value="UDP_G4E_1_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005886">
        <property type="entry name" value="UDP_G4E"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01179">
        <property type="entry name" value="galE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7 8" id="KW-0520">NAD</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epimerase" evidence="9">
        <location>
            <begin position="3"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ84169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="330" mass="36243" checksum="A81915FA27D8F7BA" modified="2013-07-24" version="1">MRILVFGGAGYIGSHTALELIRAGEEVVVADNLQTGFRAAIPEGATFYQGDIRDRAFLDDLFTKEKIDAVIHFAACSLVGESVNLPLKYYDNNLCGTKVLLEAMVAHDVKKIVFSSTAATYGEPENIPILETDRTCPTNPYGETKLAMEKMFYWTAKAHGLRYVSLRYFNACGADKTGTIGEAHNPESHLIPLILQVPNGKREAISIFGTDYDTPDGTCIRDYIHVTDLAQAHILAVRYLQDGGESNIFNLGNGIGYSVREVIETARAVTGHPIPAIESPRRAGDPARLVASSQKAREVLGWDPQHASLEEIIGDAWRWHKNHPNGYAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7V3</accession>
    <name>R5V7V3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">UDP-galactopyranose mutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_01924</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UDP-galactopyranose/dTDP-fucopyranose mutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01924"/>
    <dbReference type="EnsemblBacteria" id="CCZ84199">
        <property type="protein sequence ID" value="CCZ84199"/>
        <property type="gene ID" value="BN566_01924"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008767">
        <property type="term" value="F:UDP-galactopyranose mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004379">
        <property type="entry name" value="UDP-GALP_mutase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015899">
        <property type="entry name" value="UDP-GalPyranose_mutase_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03275">
        <property type="entry name" value="GLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00031">
        <property type="entry name" value="UDP-GALP_mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GLF" evidence="4">
        <location>
            <begin position="148"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ84199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="368" mass="42995" checksum="FF4CD3FBF869C21A" modified="2013-07-24" version="1">MKKYDYLIVGAGLFGAVFAYEAMKRGKTCLVIERRDHIAGNIYCETIEDIHVHKYGAHIFHTSNQEVWNYINQFAEFNNYINSPIAIYKDELYNLPFNMNTFSKMWNIRTPQEAKNHIAQQIAALNITEPKNLEEQALSLVGTDVYEKLIKGYTEKQWGRNCTDLPSFIIKRLPLRFTYDNNYFNDRYQGIPIGGYTPIVEKMLAGADILTNTDYFEFIKEHPDVADKTVFTGQIDAFFDYKLGVLEYRSVRFETEVLDCENYQGNAVVNYTEREVPYTRIIEHKHFEFGKQPKTVISREYSSEWKKGDEPYYPVNNERNNQMYQEYQKLAETVPNVIFGGRLGQYKYYDMDKVIAAALETVQNEFGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>R5V896</accession>
    <name>R5V896_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Metal dependent phosphohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN566_00262</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the CRISPR-associated nuclease Cas3-HD family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the central section; belongs to the CRISPR-associated helicase Cas3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000218">
        <property type="protein sequence ID" value="CCZ84349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00262"/>
    <dbReference type="EnsemblBacteria" id="CCZ84349">
        <property type="protein sequence ID" value="CCZ84349"/>
        <property type="gene ID" value="BN566_00262"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035011">
        <property type="entry name" value="Cas3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006483">
        <property type="entry name" value="CRISPR-assoc_Cas3_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038257">
        <property type="entry name" value="CRISPR-assoc_Cas3_HD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006474">
        <property type="entry name" value="Helicase_Cas3_CRISPR-ass_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF5">
        <property type="entry name" value="PTHR47959:SF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01587">
        <property type="entry name" value="cas3_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01596">
        <property type="entry name" value="cas3_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51643">
        <property type="entry name" value="HD_CAS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD Cas3-type" evidence="13">
        <location>
            <begin position="11"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="273"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="485"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CCZ84349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="805" mass="91480" checksum="DB9DE4E06F5027A0" modified="2013-07-24" version="1">MAEYISHIRESDKKEQTVSEHSNNVAKIASEFGQAVQMSHTAWLQGILHDAGKLCTDFTEYIRGKSDAKRGEIDHSYMGAKYLANLAIELYKESRLNPDAALLIGRTILSHHGLHDWLTEDGENYFEKRTSIIKQDKEVKCALPEIVSDGTLKKYYQESVKEYDAMQQKMEQLYKEMIAKGEQEKRNLSDDAKSVIPSFYQGFLERLMQSILMDADQTDAANFNDDIAYTSPKESTAVWDDMAKRMQEKCTTFRKKTDAISRQRMNISDRCAAFANHDVKICRLVVPTGGGKTLSALRFAIDYSQKHKIDQIFYIAPFNSILEQNSDVIREIAGEENFLEHHSNVFSQLEDDEKESEKLSDYQMRTERWDVPMIATTLVQFLNALFDGKSSSVRRMHHLCRAVIIIDEVQAIPAKCIHLFNLAMNFLTHICGSTVVLCSATQPCLEQAEYPILLDEQESMTADFQADFDAFRRTVLHSAGKKSFSYEDAADFCTEQYQKNGNLLLVVNTKQAALTMFQKLKERNSEFATVLHLSTNMCPAHRRTVIQQMQEQLAAHQPLICVTTQLIEAGVDISFRCVVRSMSGLDHAAQAAGRCNRNGEYDCCSVYLIQLEEEKLAAALKQIADEQEITKKILFFHEKEDLLHPEIMQCYFKNYLTTFQNQFSYSIPDLGNETLLRLLSTNSNNVKRAEAISPRLRDHAQAFRTAGKKFQVIAEQTTDILVPYGEGNDLISGLNGKLYENEYLKLLRKAQPYFVSVYSGTLKALEAKEALTRLKSDVLSLKPGYYSSEQGVCADGMISMEECFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5V8X8</accession>
    <name>R5V8X8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00405</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000242">
        <property type="protein sequence ID" value="CCZ84544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84544">
        <property type="protein sequence ID" value="CCZ84544"/>
        <property type="gene ID" value="BN566_00405"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004394800" evidence="2">
        <location>
            <begin position="32"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="14"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="270" mass="28592" checksum="BA2B16133D6D427D" modified="2013-07-24" version="1">MCGVMAIAMTASLAGCGSSDDSSSESDSSSAAETTAADSSAADESSTDADSSSDESSESSSDDSSTADESSVVYGNYEIEAEDGVDSACADVIKAYFTAIEEQDFDAYKATLYDYYYNVYNDWLQDNQGYGMETTMEQMHQSLLDSAGGDHLVVTGITVKLATAEGNADEAEEIASEEAASEETTASDSTDDTAEATTEGETEDLPTQYLGIYEQILGEGFADEVKKDVDKAFVIAFTMKGTVDGGEEKTIMDGYDLMMVEKDGAYYVLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>R5VGT9</accession>
    <name>R5VGT9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH araC/xylS-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00596</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000008">
        <property type="protein sequence ID" value="CCZ82791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00596"/>
    <dbReference type="EnsemblBacteria" id="CCZ82791">
        <property type="protein sequence ID" value="CCZ82791"/>
        <property type="gene ID" value="BN566_00596"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="1">
        <location>
            <begin position="138"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="251" mass="29550" checksum="DDE4EBF478236270" modified="2013-07-24" version="1">MATNFYQKNKSKQKVLHYPTEIRSRFYQLVSSGNLQEVEAFLEKKDFRKLCDNNVLSETSVQNMRYHFIVTVTMLTHFCIQAGMPEVQAFDIQDNYIQSADQTHRLVDMEALHDEACMEYALKMKRLQKQDISSKHIAICINYIHQNIRKKLSVPDIAEYLQLHPSYLSKLFQEQMGCSIHSYILIQKMEEAKNLLRYTDYPCRSIAASLAFSSQSHFIQLFQKYTHLTPKQYRNQQSRNNMIAEPEEIES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-09-29" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5VGV0</accession>
    <name>R5VGV0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00606</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000008">
        <property type="protein sequence ID" value="CCZ82801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82801">
        <property type="protein sequence ID" value="CCZ82801"/>
        <property type="gene ID" value="BN566_00606"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026906">
        <property type="entry name" value="LRR_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036278">
        <property type="entry name" value="Sialidase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13306">
        <property type="entry name" value="LRR_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50939">
        <property type="entry name" value="SSF50939"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004395320" evidence="2">
        <location>
            <begin position="31"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ82801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="337" mass="36575" checksum="4964A87F406EAA6B" modified="2013-07-24" version="1">MKKQLLRWFGILLCWCLFVSCFSVPARASASGTVSISCGDGETVTIDNHSFDSELHSGGLRCQALYNDKKLVMQDGNNGVAFVEQGGEAGGTAYFVYATKDAGKTWTKCTNYIKLSSTFTNVFAVDNKIILIDFNYNYDEPFRVSEFTVNPDGSYSDVLIDSQKENRAPATITYQGANTFQVAFQDGTQTTYTLSNSINGSCGTNATWTFEDGVLTISGTGEMTDDSDASPAPWNDYKDSIQKVVITSGITQIGANAFAGCSQLRLLVIPTSVTQIQTAAFSNCPQLQAIQAAEETMLDFQNIKVAEQNGSVSMLLAMQNIAFGIKQKITIAAKFQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VGW6</accession>
    <name>R5VGW6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00621</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000011">
        <property type="protein sequence ID" value="CCZ82816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82816">
        <property type="protein sequence ID" value="CCZ82816"/>
        <property type="gene ID" value="BN566_00621"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ82816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="229" mass="25564" checksum="4CC3347EDCE898E1" modified="2013-07-24" version="1">MTTNRNLIFSAVFQKGICLCEVDLRICVVTACLRSSLIQYIIWLQFVAECNAFIEVLFYQIHEGRRSQVGSHNRSTDFEVAIVNILDRGNVVLNRNDRGFCKGEVVNIQFGYIIAFKVNIDLRNFTGKAELDVGKFHPVAVRSFGCQIAVMPFTGSIGVFQTQLNAVVPFWLCKRTDGVFLVWFADVIQQKLRAMPAFAAVGYHHGRRAAVCLAIGDGNGFDTTSSGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-09-29" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5VHD1</accession>
    <name>R5VHD1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative TnpX site-specific recombinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00751</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000032">
        <property type="protein sequence ID" value="CCZ82951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82951">
        <property type="protein sequence ID" value="CCZ82951"/>
        <property type="gene ID" value="BN566_00751"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038109">
        <property type="entry name" value="DNA_bind_recomb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011109">
        <property type="entry name" value="DNA_bind_recombinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025378">
        <property type="entry name" value="DUF4368"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025827">
        <property type="entry name" value="Zn_ribbon_recom_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14287">
        <property type="entry name" value="DUF4368"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07508">
        <property type="entry name" value="Recombinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13408">
        <property type="entry name" value="Zn_ribbon_recom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00857">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="SSF53041"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51737">
        <property type="entry name" value="RECOMBINASE_DNA_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Recombinase" evidence="2">
        <location>
            <begin position="160"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="392"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ82951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="541" mass="62363" checksum="14B369E3A4E47D09" modified="2013-07-24" version="1">MTDKITALYCRLSQDDMLDGESNSITNQKAILQKFAEDNGFPNPTFYVDDGVSGTTWDRDGFKAMMSDIEDGKVATVITKDLSRLGRDYLKTGELIEMVFPDYDVRYIAVNDNVDTAKSENELLAFKNIFNDWYARDCSKKIRAVFRAKGQSGKHLCPPVYGYKKSETDKNLWVIDEPAAEVVRKIFKLCIDGYDPVQIARILTEQGIPTPTAYALLQGRDNGRHNAKTYRLGAQTIAHILERYEYCGHTVNFRTKMKSYKVHKIVYNPQEEWQIFENTQEPIITQQTFDLVQELRKHKRRPQRSEVVNPFAGMVYCADCGEKMYLSRRKNERPEQEHMRCSTYAKEQDKCTVHYIRTCVLNEIVLGELNKLLTMVRGNEEEFVKRVMSSSVQRKFSELTKAKKTLKQAEKRIAELDKLFTRLYEDNVLGRLSDERFTMMSAGYEEEQAKLKATVAELTAFVETAEQKSSDVTAFLKVVHKYEHIETLTPEIMHELIDKIIVHEPDKSSGKRVQDIEIHFRFDVAVTTISVETGKYGKKVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5VHG7</accession>
    <name>R5VHG7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00776</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000034">
        <property type="protein sequence ID" value="CCZ82976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00776"/>
    <dbReference type="EnsemblBacteria" id="CCZ82976">
        <property type="protein sequence ID" value="CCZ82976"/>
        <property type="gene ID" value="BN566_00776"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1780.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003462">
        <property type="entry name" value="ODC_Mu_crystall"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023401">
        <property type="entry name" value="ODC_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13812">
        <property type="entry name" value="PTHR13812"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02423">
        <property type="entry name" value="OCD_Mu_crystall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001439">
        <property type="entry name" value="CryM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ82976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="376" mass="41425" checksum="B093361F60E84A7E" modified="2013-07-24" version="1">MGTKVDFLYLSEPDMVKAGVKDMKQCVEVMERLLVTLNKGDYVMGGKNHNSHGCMIMFPDDPQFPGMPKNADERRFMAMPAYLGGEYQMAGMKWYGSNVENKKQGLPRSILMMMLNDKDTGAPLALMSANLVSSYRTGGIPGVGAKYLARKDAKTVAIVGPGVMGKTSLAAFVSVCPNLDTLKIKGRGQKSIDSFIEFTKKECPQIKNITVCDTLEEAVRDSDIISMTTVVRDDVASFPYINGEWVKKGALISMPSAARFDDEFLAKKCKLVVDNAKLYEAWEEEYPYPTYPQVQIVGTKFTDLVHDGLIQASDIIDITDVIQGKHAGRENDDEIIVYSVGGMPVEDIAWGGTVYRNALAKGIGVKLPLWDQPEMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>R5VHV8</accession>
    <name>R5VHV8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Xanthine phosphoribosyltransferase</fullName>
            <shortName evidence="1">XPRTase</shortName>
            <ecNumber evidence="1">2.4.2.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">xpt</name>
        <name evidence="3" type="ORF">BN566_00898</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Converts the preformed base xanthine, a product of nucleic acid breakdown, to xanthosine 5'-monophosphate (XMP), so it can be reused for RNA or DNA synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>diphosphate + XMP = 5-phospho-alpha-D-ribose 1-diphosphate + xanthine</text>
            <dbReference type="Rhea" id="RHEA:10800"/>
            <dbReference type="ChEBI" id="CHEBI:17712"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.22"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; XMP biosynthesis via salvage pathway; XMP from xanthine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the purine/pyrimidine phosphoribosyltransferase family. Xpt subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.22" evidence="1"/>
    <dbReference type="EMBL" id="CBAR010000059">
        <property type="protein sequence ID" value="CCZ83111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00898"/>
    <dbReference type="EnsemblBacteria" id="CCZ83111">
        <property type="protein sequence ID" value="CCZ83111"/>
        <property type="gene ID" value="BN566_00898"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00602">
        <property type="reaction ID" value="UER00658"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000310">
        <property type="term" value="F:xanthine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046110">
        <property type="term" value="P:xanthine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032265">
        <property type="term" value="P:XMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01184">
        <property type="entry name" value="XPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010079">
        <property type="entry name" value="Xanthine_PRibTrfase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01744">
        <property type="entry name" value="XPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="54"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="5-phospho-alpha-D-ribose 1-diphosphate binding" evidence="1">
        <location>
            <begin position="128"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>xanthine</name>
            <dbReference type="ChEBI" id="CHEBI:17712"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>xanthine</name>
            <dbReference type="ChEBI" id="CHEBI:17712"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>xanthine</name>
            <dbReference type="ChEBI" id="CHEBI:17712"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="202" mass="21918" checksum="4DBA29711EF396B9" modified="2013-07-24" version="1">MNFLEERIQKDGIVKEGNILKVDSFLNHQMDVALFRQMGEEFQKRFAGKQINKILTIEASGIGIACIVAGCFGVPVVFAKKSKSVNIDGAVYVAEVESFTHKCKNQVIVSKKFLHPEDRVLIIDDFLANGCALQGLISIVQQAGATVEGIGIAIEKGFQQGGKIIRNLGYQLESLAIVDAMDAATGKIQFRSQETGEAATAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJ86</accession>
    <name>R5VJ86_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01334</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000131">
        <property type="protein sequence ID" value="CCZ83566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01334"/>
    <dbReference type="EnsemblBacteria" id="CCZ83566">
        <property type="protein sequence ID" value="CCZ83566"/>
        <property type="gene ID" value="BN566_01334"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032681">
        <property type="entry name" value="HlyD_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12700">
        <property type="entry name" value="HlyD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="413" mass="45441" checksum="C433E92F3D8754E2" modified="2013-07-24" version="1">MMHILLIVLSIAIIATIGTIFYHFVDTQYKTETAVSATAEQSETFQGVYVRDETVLTYNGSGAISYNVPDGGKVEKGGTIAEIYADQSAIETNQQIAALQQELNLLNRISNPGILQNAQPAELSTQVKRYYNQITYCKDSGNLSGLQAAKSDFQIALSAYQLVVNQGAENFSEQATAVSEEITKLQNSKQAPRDTVKAASPVYFVSYADGYESKLTTDNIDKLTIDDIKAVQNNNGLQSDTIVGKTIDSYGWYMVGVIDNTTLEYQVDDEVTLKLANSGATANAVITDLRTSSDPKETLIVLHCDYMTSDFVKNRSERVEIIKGEYEGIQVPRSAIRFKELEETSTNVLTGEETTTKVNYRGVYVMDGETVTFRKLDVIYEGDDYVLSSLNAGDGYLILYDSIIVEGIDVNGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJ99</accession>
    <name>R5VJ99_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">SSS sodium solute transporter superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_01354</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000134">
        <property type="protein sequence ID" value="CCZ83586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01354"/>
    <dbReference type="EnsemblBacteria" id="CCZ83586">
        <property type="protein sequence ID" value="CCZ83586"/>
        <property type="gene ID" value="BN566_01354"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="76"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="160"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="237"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="274"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="335"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="381"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="412"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="443"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="476"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="519" mass="55585" checksum="545A8A73B598D6E0" modified="2013-07-24" version="1">MTTAITLAMLIVYIAIMMGIGIYTASKTKSVSDFVLGGHSVGSWLTAFAYGTSYFSAVVFIGYAGQFGWNYGVSSTWIGIGNAVLGSALAWLVLGRRTRLMSNHLQAKTMPEFFGNRFQSVSLKIAAALIVFVFLIPYTASVYNGLSRLFNMAFDLESDASYRIIIIAMAIVTAIYVILGGYAATALNDFIQGIVMLIGIAAVVVCTLQNHGGFSESLRQMGEIEQGQYATLFGPEPLNLLGVVILTSLGTWGLPQMVHKFYAIKNTDGIRKGAVISTIFALVVAGGSYFMGGFDRLFCTLNGESGKTAIATLANGKPEYDAMVPAMLQSALPDLLIGLVVVLVLSASMSTLSSLVLTSSSTMTIDFIRPRMKHHSEKKEVIIMRVLIAAFLLISVLIAFNKNASISTLMSYSWGALAGSFLGPFVFGLYSKKVTVPAVWTSFILGVGGTIGHMILFGFGWFPALTKSAASLPLNLASPINIGAILMILSLIVVPVISSFTKVKDPKTTDAAFTCYQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJL0</accession>
    <name>R5VJL0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TrpR family protein YerC/YecD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01461</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000158">
        <property type="protein sequence ID" value="CCZ83726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01461"/>
    <dbReference type="EnsemblBacteria" id="CCZ83726">
        <property type="protein sequence ID" value="CCZ83726"/>
        <property type="gene ID" value="BN566_01461"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000831">
        <property type="entry name" value="Trp_repress"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010921">
        <property type="entry name" value="Trp_repressor/repl_initiator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038116">
        <property type="entry name" value="TrpR-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013368">
        <property type="entry name" value="YecD_YerC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40080">
        <property type="entry name" value="PTHR40080"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01371">
        <property type="entry name" value="Trp_repressor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF012508">
        <property type="entry name" value="YerC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48295">
        <property type="entry name" value="SSF48295"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02531">
        <property type="entry name" value="yecD_yerC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="103" mass="11774" checksum="9E72496FE60609F2" modified="2013-07-24" version="1">MKDAQLEEDLQALAKAFLLLKTEEECTAFLKDVCTYQELRALSQRLHVARLLRKQYVFHEIVQETGASTATISRVNRTLRDSTGGYQAVLERMEPETDTKPES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJW8</accession>
    <name>R5VJW8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Fructose-2 6-bisphosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01571</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000166">
        <property type="protein sequence ID" value="CCZ83836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01571"/>
    <dbReference type="EnsemblBacteria" id="CCZ83836">
        <property type="protein sequence ID" value="CCZ83836"/>
        <property type="gene ID" value="BN566_01571"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="SSF53254"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="176" mass="19830" checksum="D4C425616D6B8EA8" modified="2013-07-24" version="1">MPHLYFVRHGQTIWNVENKICGSTDIALTPLGHEQAIAAGKLILERQYPIDAILYSPLMRAADTAKHIAEVTGLPAFAEPRLREQNFGKYESTARDGAAFQQAKTQFANHYEGGESMLQLAQRIYNLLDELLQQEKNYLLVAHNGIARVVRSYFQDMTNEAYAKYGIGNCEVLQLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5VK93</accession>
    <name>R5VK93_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GCN5-related N-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01675</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000175">
        <property type="protein sequence ID" value="CCZ83946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01675"/>
    <dbReference type="EnsemblBacteria" id="CCZ83946">
        <property type="protein sequence ID" value="CCZ83946"/>
        <property type="gene ID" value="BN566_01675"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="164" mass="19308" checksum="425FDFFBF59C484D" modified="2013-07-24" version="1">MIIIRSVKTAEQIQQLADLANKIWNEYFVKILSKEQIAYMVEQFQSNTALTKQIEEGYQYYFVIDDTEVVGYFGICKKPDDTMFLSKFYLSGEHRGKGYASQMFQFIKEKALEQRCSNIWLTVNRYNQQAIAVYEHFGMQCVQKQVTDIGNGFVMDDFVYRYTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5VL10</accession>
    <name>R5VL10_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_01951</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84226">
        <property type="protein sequence ID" value="CCZ84226"/>
        <property type="gene ID" value="BN566_01951"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009403">
        <property type="term" value="P:toxin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003825">
        <property type="entry name" value="Colicin-V_CvpA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02674">
        <property type="entry name" value="Colicin_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="217"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ84226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="271" mass="29139" checksum="2EB8727E1404EDE5" modified="2013-07-24" version="1">MAWYYDLVVLAILALCVWNGMRKGVSRSLLQTAATILSAVVALWVSQPLAELCYTEFLEKPVVSAVEGQLNTVDVTGLVQTALSDNGIPVSREQVQSMLADPEGTAAQYGFDSGWVNQQIDAAADSVEELTNGVLPSWLMKEIAGDGAVQKKTAKSVISGDTHTAAQQLVSTYGKPILCSVLRSILFGLCFGVLMLLFQAVLFLIPVQQMGLSVDLVVGALAGLAKAGILLWMMVVLTKVVVSATEGTTPFFTEATIQKTYLFQLLYELKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5VL40</accession>
    <name>R5VL40_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00190</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RelB/DinJ antitoxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000203">
        <property type="protein sequence ID" value="CCZ84261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00190"/>
    <dbReference type="EnsemblBacteria" id="CCZ84261">
        <property type="protein sequence ID" value="CCZ84261"/>
        <property type="gene ID" value="BN566_00190"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013321">
        <property type="entry name" value="Arc_rbn_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007337">
        <property type="entry name" value="RelB/DinJ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04221">
        <property type="entry name" value="RelB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02384">
        <property type="entry name" value="RelB_DinJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="89" mass="10030" checksum="9B9FB6FDB2F1CB92" modified="2013-07-24" version="1">MAQATISARIDEKDKQAFDNFCSDVGLNTSAAINLFIKAVLRERRIPFEISQSSDPFYSESNQKHLMKAIQELRDGKGTAHDLIEVDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>R5VL43</accession>
    <name>R5VL43_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cell surface protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00195</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000204">
        <property type="protein sequence ID" value="CCZ84266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00195"/>
    <dbReference type="EnsemblBacteria" id="CCZ84266">
        <property type="protein sequence ID" value="CCZ84266"/>
        <property type="gene ID" value="BN566_00195"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002105">
        <property type="entry name" value="Dockerin_1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026906">
        <property type="entry name" value="LRR_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00404">
        <property type="entry name" value="Dockerin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13306">
        <property type="entry name" value="LRR_5"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004395233" evidence="2">
        <location>
            <begin position="38"/>
            <end position="742"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="742" mass="79682" checksum="D5DD02404F8596DE" modified="2013-07-24" version="1">MKGESFMKKILRKGISCILSMLVLLQGWLFVPNGAAALEEDWSPETTTAATTENVGQVQEWEQFQYQVSENEVTIVGYVGTIEGTLEIPAEIDGLPVTRVEDDAFIKQNQLVEVVIPASVIEIGSTAFHDCENLQVFTVAEENPVYCVKNGLLLDKGEKVLIRCPSGRYGEVTDIPNTVFLISDAAFADCSSLSKIELPVSVLSIGAMAFSNCTALYDITLPKDLQTIGDGAFMNCTAIQRMILPENLRRIGMMAFSNCQSLSEVIVPSGVQTLPDSAFADCIALQRVTLSEGLTNISVAAFKNCTALQEVQLPDTLESIKSNAFENCSSLQRMHFPAAVSELSANAFSGCISLSEVTVAAENPYYTAVDGVLMTADQATLVYYPPCSSQSDYTIPETVKTIDSYAFQDVMQLQFLTVPETVETIEPYACYDANSLKEVMLNGASVISAYAFQSCDQLEAVVFGDGVQEVQTGAFLDCPSLKSIILPKTITTIEDYAFGYQTDSAEAGTYSMVKGFFICFYKYTAGAYYAALNGFDSLSLDDVITTTTTAVSANVTTTTKTKTETTASNQTTAAVTTAALETTSKLETSQATATKAETTAVVTTTTIAATTNRPETSIVSTTTPQFVTTTTTTTHQHGGTATTVTTGVTTQTTAVTGQTTQVTTELKGDLNLDGRLSIVDAIRVLTYYAKMASGQAAYLYSEDPDVEEAAKRRVDMDGNGRIDTTDAVEILIIYAKTAAGQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VQE5</accession>
    <name>R5VQE5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00617</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000009">
        <property type="protein sequence ID" value="CCZ82812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82812">
        <property type="protein sequence ID" value="CCZ82812"/>
        <property type="gene ID" value="BN566_00617"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ82812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="279" mass="30968" checksum="4E27A2AC892CA197" modified="2013-07-24" version="1">MAPSISVRCTLFVIAILFAGIFASCLPVRERLAARAERSGGDYRAECGILTSIALAEYLYHSHTNTGNNIARSSFCTRGWLFAVSVLEAATIRRNLKNRRNAPPISVRCTLLIRQRMSCSESRAFGWRLLNRTPKPCKVRSCRTSIPFPHNTRNNVARSGFCTEGWLFAVSVLGTSAIRGNLKKQKKCTACFSTVHIAYQAENALQREPGVRVAITEQNAEALQGSLLQNTDTIPTQYRKQHCPFRFLHRRTAVCCICFGSCCNQAGFEYIGGIESSSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>R5VQR4</accession>
    <name>R5VQR4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 13">DNA primase</fullName>
            <ecNumber evidence="12">2.7.7.101</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">dnaG</name>
        <name evidence="16" type="ORF">BN566_00709</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 13">RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12 13 14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="12 13 14">Binds 1 zinc ion per monomer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Monomer. Interacts with DnaB.</text>
    </comment>
    <comment type="domain">
        <text evidence="12">Contains an N-terminal zinc-binding domain, a central core domain that contains the primase activity, and a C-terminal DnaB-binding domain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12 13">Belongs to the DnaG primase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.101" evidence="12"/>
    <dbReference type="EMBL" id="CBAR010000022">
        <property type="protein sequence ID" value="CCZ82907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00709"/>
    <dbReference type="EnsemblBacteria" id="CCZ82907">
        <property type="protein sequence ID" value="CCZ82907"/>
        <property type="gene ID" value="BN566_00709"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003896">
        <property type="term" value="F:DNA primase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03364">
        <property type="entry name" value="TOPRIM_DnaG_primases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.860.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.980.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00974">
        <property type="entry name" value="DNA_primase_DnaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036185">
        <property type="entry name" value="DNA_heli_DnaB-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016136">
        <property type="entry name" value="DNA_helicase_N/primase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013264">
        <property type="entry name" value="DNA_primase_core_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037068">
        <property type="entry name" value="DNA_primase_core_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019475">
        <property type="entry name" value="DNA_primase_DnaB-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006295">
        <property type="entry name" value="DNA_primase_DnaG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036977">
        <property type="entry name" value="DNA_primase_Znf_CHC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030846">
        <property type="entry name" value="DnaG_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034151">
        <property type="entry name" value="TOPRIM_DnaG_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002694">
        <property type="entry name" value="Znf_CHC2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10410">
        <property type="entry name" value="DnaB_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08275">
        <property type="entry name" value="Toprim_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01807">
        <property type="entry name" value="zf-CHC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002811">
        <property type="entry name" value="DnaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00400">
        <property type="entry name" value="ZnF_CHCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48024">
        <property type="entry name" value="SSF48024"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01391">
        <property type="entry name" value="dnaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12 13" id="KW-0235">DNA replication</keyword>
    <keyword evidence="10 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1 12 13" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 12 13" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="2 12 13" id="KW-0639">Primosome</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 12 13" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 12 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 12 13" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 12 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Toprim" evidence="15">
        <location>
            <begin position="250"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CHC2-type" evidence="12 14">
        <location>
            <begin position="37"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR002811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR002811-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CCZ82907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="582" mass="65804" checksum="BE1AB74E4CA8613E" modified="2013-07-24" version="1">MVSDVFLEQLRNACPLEEIARTYVDLKRRGRTYVCNCPFHSEKTPSFTVFPDTQSFYCFGCGAGGDVITFIQQIENLDFMDAVKLLAQNSGMTVPEQGADEKQSRMRKRILAVNRAAANFYYRMLTGSDKRGLQYFIQRHLTTTTIKKYGLGFAPETWDALSRYLLQQGFTEEELLLADVSRRSRRGTLIDTFRNRVIFPIVDLRGNVIGFGGRVLDDSQPKYLNTAQTPVFDKGKNLFSLQFAKNSVTTTLILAEGYMDVIAVHQAGFENVVATLGTAITPDQARKISQYAKTVIIAYDSDAAGQAATQKAMRHFSEVGIETKILRMQGAKDPDEYIRKFGAERFRMLLEGSEDAINFQLDRCEMGLDLQTENGRATLLRRATEVLIQIPDPVTREVYLMRTAEKCHISADVLRVSVNRKQKNRQKMQKKKEFSNLVAHTVTAQQTAADVSGMAGSSRTVRAQERLLCYFLSQPEQISKLADHLQPEQFTVPFYQKLFQLLLEHQQQESDGWISALGMQLTPQEMGTVSRLLNLKQTCPISPQEVQDCIQLLQTHLVQALDSDAALLELVAQKKKKNQAWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5VR73</accession>
    <name>R5VR73_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted glutamine amidotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00835</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000040">
        <property type="protein sequence ID" value="CCZ83042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00835"/>
    <dbReference type="EnsemblBacteria" id="CCZ83042">
        <property type="protein sequence ID" value="CCZ83042"/>
        <property type="gene ID" value="BN566_00835"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011698">
        <property type="entry name" value="GATase_3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07685">
        <property type="entry name" value="GATase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GATase_3" evidence="1">
        <location>
            <begin position="6"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="239" mass="26719" checksum="C3C25310B19DD70B" modified="2013-07-24" version="1">MNTCVIEALYPEVCNLFGDTGNLRYLKLCLPQATFIETALNDEPYFVHHPVSMIYLGPMSEHTQCTVISKLAPYRERIAECIANGVTFLATGNAMEVFGKQITDAQSGVTTGLGLLDLTTTRDMMHRFYSLVLGTYNELQMVGFRAQFTRSTLGSTEVPFIQVTKGTPMCETARIDGIQKNHFYGTYLLGPLLILNPYFTKQLLQQITGESVPLAFEKETIAAYHARLADFQDKRVGIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>R5VR92</accession>
    <name>R5VR92_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transcriptional repressor NrdR</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">nrdR</name>
        <name evidence="10" type="ORF">BN566_00855</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Negatively regulates transcription of bacterial ribonucleotide reductase nrd genes and operons by binding to NrdR-boxes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the NrdR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000050">
        <property type="protein sequence ID" value="CCZ83067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00855"/>
    <dbReference type="EnsemblBacteria" id="CCZ83067">
        <property type="protein sequence ID" value="CCZ83067"/>
        <property type="gene ID" value="BN566_00855"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00440">
        <property type="entry name" value="NrdR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005144">
        <property type="entry name" value="ATP-cone_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003796">
        <property type="entry name" value="RNR_NrdR-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30455">
        <property type="entry name" value="PTHR30455"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03477">
        <property type="entry name" value="ATP-cone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00244">
        <property type="entry name" value="TIGR00244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51161">
        <property type="entry name" value="ATP_CONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0678">Repressor</keyword>
    <keyword evidence="6 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="ATP-cone" evidence="9">
        <location>
            <begin position="47"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ83067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="162" mass="18317" checksum="15F946D59CF91FC1" modified="2013-07-24" version="1">MQCPFCGARDTKVLDSRPAEHRIRRRRECIFCGKRFTTYEMVERPLLMVKKRDGSFEPFDRQKLITGIFNAIKKRPVSVEQVAAIAEGIEQQCANAMRSTVTSVDIGNWVMEHLKQIDAVAYIRFASVYEDFTDVASFVAAISALDEETAAAKMAESKEEGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5VRR6</accession>
    <name>R5VRR6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Fe2+ transport system protein A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01033</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000082">
        <property type="protein sequence ID" value="CCZ83252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01033"/>
    <dbReference type="EnsemblBacteria" id="CCZ83252">
        <property type="protein sequence ID" value="CCZ83252"/>
        <property type="gene ID" value="BN566_01033"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007167">
        <property type="entry name" value="Fe-transptr_FeoA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038157">
        <property type="entry name" value="FeoA_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008988">
        <property type="entry name" value="Transcriptional_repressor_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04023">
        <property type="entry name" value="FeoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00899">
        <property type="entry name" value="FeoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50037">
        <property type="entry name" value="SSF50037"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FeoA" evidence="2">
        <location>
            <begin position="2"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="70" mass="7606" checksum="12EDFAC229E3AA55" modified="2013-07-24" version="1">MMPLTFANVGEENCIRKIGGNPETKKFLENLGFVVGGSVIVVNEINGNMIVNVKESRVAISKEMANKILV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5VRU1</accession>
    <name>R5VRU1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Predicted metal-dependent protease of the PAD1/JAB1 superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_01058</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01058"/>
    <dbReference type="EnsemblBacteria" id="CCZ83277">
        <property type="protein sequence ID" value="CCZ83277"/>
        <property type="gene ID" value="BN566_01058"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070122">
        <property type="term" value="F:isopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140492">
        <property type="term" value="F:metal-dependent deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028090">
        <property type="entry name" value="JAB_dom_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14464">
        <property type="entry name" value="Prok-JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 8" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MPN" evidence="7">
        <location>
            <begin position="2"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="137" mass="15383" checksum="635030286818BA7D" modified="2013-07-24" version="1">MITLKKADFEKILAHAKKELPNEACGLIAGVKDGADKHIQKVYLLTNIDHSNEHFSLDPKEQLAAIKDMRANGLVPLGNWHSHPESPSRPSQEDKRLAYDKTASYMILSLMELDQPVLNSFHIEGDDAEKEPLVIEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>R5VS00</accession>
    <name>R5VS00_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN566_01113</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CBAR010000089">
        <property type="protein sequence ID" value="CCZ83332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01113"/>
    <dbReference type="EnsemblBacteria" id="CCZ83332">
        <property type="protein sequence ID" value="CCZ83332"/>
        <property type="gene ID" value="BN566_01113"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="89"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="9">
        <location>
            <begin position="109"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="8">
        <location>
            <begin position="170"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ83332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="387" mass="43289" checksum="BC4DF977FEEA083C" modified="2013-07-24" version="1">MKRMKRLSFQWRITLLTAILIAGTCICLNLLLYRSGTVSMDSLNGFVMKYDPGNSDVVKIEFDDQQMSAFLHQFSQEVYDAKAAFGKKGWCITAVVTLLSAAIAYFVSGRALKPLKQFSQQTENINQDNLTSTRLDEDTAAEFQQLSCSVNRMLDRLALSFDLQRQFAGNAAHELRTPLAMMQTKLELFAEEHSNINTETAELVQFQMAQVDRLTALVRTLLEMSNLQSVSRSDEIELAPLIDEILTDLTTLAEQKNITFHQNCEDICMIGSDTLIYRLLFNLIENAIKYNHAGGSVTVSMQQENHQVLLHIADTGCGISEEYRESIFQPFFRVDKSRSRQMGGVGLGLALVREIAVLHGGSVCVESSSEKGTTFAVRLPLNGEKTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>R5VTM1</accession>
    <name>R5VTM1_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">50S ribosomal protein L11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rplK</name>
        <name evidence="9" type="ORF">BN566_01601</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 6">Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the ribosomal stalk of the 50S ribosomal subunit. Interacts with L10 and the large rRNA to form the base of the stalk. L10 forms an elongated spine to which L12 dimers bind in a sequential fashion forming a multimeric L10(L12)X complex.</text>
    </comment>
    <comment type="PTM">
        <text evidence="4 6">One or more lysine residues are methylated.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the universal ribosomal protein uL11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000169">
        <property type="protein sequence ID" value="CCZ83872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01601"/>
    <dbReference type="EnsemblBacteria" id="CCZ83872">
        <property type="protein sequence ID" value="CCZ83872"/>
        <property type="gene ID" value="BN566_01601"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00349">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00736">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000911">
        <property type="entry name" value="Ribosomal_L11/L12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036796">
        <property type="entry name" value="Ribosomal_L11/L12_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006519">
        <property type="entry name" value="Ribosomal_L11_bac-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020783">
        <property type="entry name" value="Ribosomal_L11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036769">
        <property type="entry name" value="Ribosomal_L11_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020784">
        <property type="entry name" value="Ribosomal_L11_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661">
        <property type="entry name" value="PTHR11661"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00298">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03946">
        <property type="entry name" value="Ribosomal_L11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00649">
        <property type="entry name" value="RL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46906">
        <property type="entry name" value="SSF46906"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54747">
        <property type="entry name" value="SSF54747"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01632">
        <property type="entry name" value="L11_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0488">Methylation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 6" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Ribosomal_L11_N" evidence="8">
        <location>
            <begin position="9"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal_L11" evidence="7">
        <location>
            <begin position="71"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ83872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="141" mass="14904" checksum="89ED4BBDEBF4873C" modified="2013-07-24" version="1">MAQKVTGYIKLQIPAGKATPAPPVGPALGQHGVNIMAFTKEFNERTKNDIGMIIPVVITVYADRSFTFITKTPPAAVLLKKACGLETASGVPNKTKVANITKEQIQQIAEHKMPDLNAGSLEAAMSMIAGTARSMGIVVVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>R5VTV2</accession>
    <name>R5VTV2_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fumarate hydratase class II</fullName>
            <shortName evidence="3">Fumarase C</shortName>
            <ecNumber evidence="3">4.2.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Aerobic fumarase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Iron-independent fumarase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">fumC</name>
        <name evidence="6" type="ORF">BN566_01676</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(S)-malate = fumarate + H2O</text>
            <dbReference type="Rhea" id="RHEA:12460"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="EC" id="4.2.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="3">There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.2" evidence="3"/>
    <dbReference type="EMBL" id="CBAR010000175">
        <property type="protein sequence ID" value="CCZ83947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01676"/>
    <dbReference type="EnsemblBacteria" id="CCZ83947">
        <property type="protein sequence ID" value="CCZ83947"/>
        <property type="gene ID" value="BN566_01676"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER01007"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045239">
        <property type="term" value="C:tricarboxylic acid cycle enzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004333">
        <property type="term" value="F:fumarate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006106">
        <property type="term" value="P:fumarate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01362">
        <property type="entry name" value="Fumarase_classII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00743">
        <property type="entry name" value="FumaraseC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005677">
        <property type="entry name" value="Fum_hydII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018951">
        <property type="entry name" value="Fumarase_C_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11444">
        <property type="entry name" value="PTHR11444"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10415">
        <property type="entry name" value="FumaraseC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="SSF48557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00979">
        <property type="entry name" value="fumC_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Lyase_1" evidence="4">
        <location>
            <begin position="11"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="FumaraseC_C" evidence="5">
        <location>
            <begin position="406"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="3">
        <location>
            <begin position="101"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding (B site)" evidence="3">
        <location>
            <begin position="127"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="3">
        <location>
            <begin position="137"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="3">
        <location>
            <begin position="322"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="3">
        <location>
            <position position="186"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="316"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="317"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="3">
        <location>
            <position position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF10415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ83947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="458" mass="49872" checksum="D7A5B776660B4627" modified="2013-07-24" version="1">MEYRIEHDSMGEVKVPADKYWAAQTQRSSQNFPIGVGLETMPREITHAFGILKKAAAMANHSLKPEKMTDEKLAAISQACDEVMSGSLNAHFPLVVWQTGSGTQSNMNANEVIANRGNEIAGKKLLHPNDDVNMSQSSNDTFPTAMHISAVIAIEDKVFPAIDKLVATFQRLETENEGIVKSGRTHLQDATPIAFSQEISGWRSTLERDKEMLQAALPFLKTLALGGTAVGTGLNTPKGFDVAVAKAVSEITGKEFRTAENKFHALTSKDELVFAHGALKALAADMMKIANDVRWLASGPRDGLGEIFIPENEPGSSIMPGKVNPTQCEAVTMVAVQVMGNDVAVGMAASQGNFELNVFMPVCMYNFLQSARLLAEAIVSFNDHCACGIRANKEKMHHNLHNSLMLVTALNPYIGYENAAKTAKKAYKENISLKEACVSLGFLTAERFDEVFHPEQMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5VU42</accession>
    <name>R5VU42_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">zinc_ribbon_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01748</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000180">
        <property type="protein sequence ID" value="CCZ84022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84022">
        <property type="protein sequence ID" value="CCZ84022"/>
        <property type="gene ID" value="BN566_01748"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026870">
        <property type="entry name" value="Zinc_ribbon_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13240">
        <property type="entry name" value="zinc_ribbon_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004395718" description="zinc_ribbon_2 domain-containing protein" evidence="2">
        <location>
            <begin position="29"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="197"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="zinc_ribbon_2" evidence="3">
        <location>
            <begin position="239"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="262" mass="28178" checksum="5744664D9ECF10BD" modified="2013-07-24" version="1">MKKTISRIGCFLLAILLGLYSVAIPADADALSPGEWFVTGYYRVTSDGVMGYNGRYVMDGGAQEIESSLYAGQVVYARIAYSSMGVTWYSCSSEDEHGDGFNYVWYGWVDASYLVPVGQEPEPEPETDPPTEAPTTVVTTTTTTTTTTTTTTTVTSTTATTVSNTTVPTTTETTTTTTQAIVPANHKQNSHFISDNLLLILIGCIVLLLACSAGLAIVLVHRSKAMVSLQNSDNLKPSFCPYCGAKHEDGVKFCKKCGKELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>R5VUJ4</accession>
    <name>R5VUJ4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transport permease protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_01922</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ABC-2 integral membrane protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01922"/>
    <dbReference type="EnsemblBacteria" id="CCZ84197">
        <property type="protein sequence ID" value="CCZ84197"/>
        <property type="gene ID" value="BN566_01922"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC_2_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000412">
        <property type="entry name" value="ABC_2_transport"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51012">
        <property type="entry name" value="ABC_TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="60"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="110"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="139"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="169"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="223"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="ABC2_membrane" evidence="7">
        <location>
            <begin position="12"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ84197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="255" mass="30491" checksum="1E4B3775F6632C22" modified="2013-07-24" version="1">MIKTLKQHQFLFRELVKRDFRQKYKKTTLGFIWSILNPLAEFLVLMLIFKNLFSRNTPHYTIYMLVGILTYSYYSNASQTGMQSFLGNAGIIQKIKVPTWIFPLSKNVSALFNFFITLILLIPFMIYDHMGFTWKLVFLIYPIILMFFFNLGVSMILASLYVFFKDIQYFYNIFCRLLYFCCAVFWYDTSMSETAQKILHINPIYDFIYYSRMVIIHQQIPTLFDHLVLLGYTILFLGLGILIYRCNKDKFVYYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5VUQ0</accession>
    <name>R5VUQ0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ABC-type uncharacterized transport system</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00016</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000197">
        <property type="protein sequence ID" value="CCZ84247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00016"/>
    <dbReference type="EnsemblBacteria" id="CCZ84247">
        <property type="protein sequence ID" value="CCZ84247"/>
        <property type="gene ID" value="BN566_00016"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019196">
        <property type="entry name" value="ABC_transp_unknown"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09822">
        <property type="entry name" value="ABC_transp_aux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="527"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="domain" description="ABC_transp_aux" evidence="2">
        <location>
            <begin position="53"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="554" mass="61135" checksum="9E6C711E1BE2547D" modified="2013-07-24" version="1">MEQQDAKLIKQRQRKKKMKYGGIAFAITVIVTAIVILVNVVVGLLVERYPNAKIDLTSSNLYEVSDETIDYIKNLTEPVEIAVSSEESTLEKDSYLKMVTELLKKYQGYSSEIEVTYFDTTKDPDILSKYQSKYSGTISSDNVIVCSGDRVKVFPLSDFFDMDQDKLQYYYYGQVSLSECITAFKGEQSLTNAIMNVTDANPQRVGFIGTSNGNTIYSPTQGNQYAAKVLSTLLDDNGYDVTQLDMVTDTISPDDYDLLVLPAPVNDLTVDAIDKLETFLHNDGNLGKRLLYIADFTQGNTPNLDAFLKDWNIVVDNSYVIDDNSSTQQAVTLLVAGNQRMYAPIVNQTSSDYSSGLSNTSLPIIAPVSRPILTRDANNGRVVTDLLDTADSAYCVPLNLDSNSERAEAAWDAQNGVTTTEATEETTEETTTTTTFDAANAERSSYSVMAFSQNQAAQDNGQIESDVMVIGSMGYFDYYVAQDTSYNNADYFISALNTICGKSDNIVITAKDMNITSMNTTASQMKLLTTVVLVVFPAITIVIGIVIWARRKSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>R5VUU8</accession>
    <name>R5VUU8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Glutamine--fructose-6-phosphate aminotransferase [isomerizing]</fullName>
            <ecNumber evidence="2 8">2.6.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">D-fructose-6-phosphate amidotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">GFAT</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Glucosamine-6-phosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Hexosephosphate aminotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">L-glutamine--D-fructose-6-phosphate amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">glmS</name>
        <name evidence="11" type="ORF">BN566_00036</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>D-fructose 6-phosphate + L-glutamine = D-glucosamine 6-phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:13237"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="EC" id="2.6.1.16"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.16" evidence="2 8"/>
    <dbReference type="EMBL" id="CBAR010000208">
        <property type="protein sequence ID" value="CCZ84297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00036"/>
    <dbReference type="EnsemblBacteria" id="CCZ84297">
        <property type="protein sequence ID" value="CCZ84297"/>
        <property type="gene ID" value="BN566_00036"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004360">
        <property type="term" value="F:glutamine-fructose-6-phosphate transaminase (isomerizing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901137">
        <property type="term" value="P:carbohydrate derivative biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05008">
        <property type="entry name" value="SIS_GlmS_GlmD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05009">
        <property type="entry name" value="SIS_GlmS_GlmD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00164">
        <property type="entry name" value="GlmS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035466">
        <property type="entry name" value="GlmS/AgaS_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035490">
        <property type="entry name" value="GlmS/FrlB_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005855">
        <property type="entry name" value="GlmS_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937:SF0">
        <property type="entry name" value="PTHR10937:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SSF53697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01135">
        <property type="entry name" value="glmS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8 11" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 8 11" id="KW-0808">Transferase</keyword>
    <feature type="initiator methionine" description="Removed" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="9">
        <location>
            <begin position="2"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="10">
        <location>
            <begin position="284"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="10">
        <location>
            <begin position="456"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; for GATase activity" evidence="8">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="active site" description="For Fru-6P isomerization activity" evidence="8">
        <location>
            <position position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ84297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="607" mass="66577" checksum="B70BF9DA199D7588" modified="2013-07-24" version="1">MCGIVGYVGSRNCTDVLINGLTKLEYRGYDSAGIAVFSESGNIQVAKSKGRLADLIHKMEQEGRPEGHAGIGHTRWATHGEPSDINSHPHTGGRVTIVHNGIIENYKELKEYLSAKGVTFASDTDTEVVARLLDYNYNGSPIETISNTIRDLEGSYALGIIFADHPDVVYATRKESPLIVGLGKEESFIASDVPAILRYTRDYYLLEQEEIAVLDHSGVKVYDAYGVEVQKERKTADWDMDAAEKGGYAHFMLKEIHEQPTAIKTTITPRIRNGLPNLEECNITPEALKQYHRIFVVACGTAMHAGVVGQYVIEKLARIPVTVDIASEFRYRDPILSAEDLVIIISQSGETADSLAAMRLAKNRGAKTLAIVNAKGSSIAREADMLIYTHAGPEIAVASTKAYMVQIAVMYLLAFEFALAAGTIDEIECQKLVAELQQTPDYISEILKNKEQTQFIASSLITADSLLYIGRGLDYALSMEGSLKLKEISYIHSESYAAGELKHGTISLISDGMPVIAVATQKALFEKTISNIKEVKARGAKVVLVCRDTYQPDTDVADLKFGLPDFDDLLMPMLAVVPLQLIAYYTSVLRGNDVDKPRNLAKSVTVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>R5VV69</accession>
    <name>R5VV69_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Peptidyl-prolyl cis-trans isomerase (Rotamase)-cyclophilin family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00314</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000224">
        <property type="protein sequence ID" value="CCZ84407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00314"/>
    <dbReference type="EnsemblBacteria" id="CCZ84407">
        <property type="protein sequence ID" value="CCZ84407"/>
        <property type="gene ID" value="BN566_00314"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000413">
        <property type="term" value="P:protein peptidyl-prolyl isomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="SSF50891"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004407399" evidence="1">
        <location>
            <begin position="35"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="2">
        <location>
            <begin position="64"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="284" mass="30971" checksum="62B667A2950BB1B2" modified="2013-07-24" version="1">MAKLSKKGISTLLVLLVVVSLLSLTMFSCTIRMAQKDSTYIDMGKAHLLQLETPADDAPAMIVHTSVGDITAVLYPEVAPNYVAQFTELVEKGYYDGTYVYQTEPGVYFRAGSPNADGTLDDQLDESRQKVETETSADLWPFRGAFCAPVTEKDNNFFKMLFGNDVSYCGTRFLVCNTIEFDEETKTELADTDESAAAVTDTFLSWGGIPNYAQQMTIFAQAYGKESFSVIDTITGAATPSENGTTATTVSTAKSDAPIQIETITLTTWGETEQDAYVPENSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVD5</accession>
    <name>R5VVD5_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dockerin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00361</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000238">
        <property type="protein sequence ID" value="CCZ84462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00361"/>
    <dbReference type="EnsemblBacteria" id="CCZ84462">
        <property type="protein sequence ID" value="CCZ84462"/>
        <property type="gene ID" value="BN566_00361"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002105">
        <property type="entry name" value="Dockerin_1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00404">
        <property type="entry name" value="Dockerin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004407360" description="Dockerin domain-containing protein" evidence="2">
        <location>
            <begin position="39"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="3">
        <location>
            <begin position="69"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="141" mass="14311" checksum="62579755A946752A" modified="2013-07-24" version="1">MPDETTTTTTTTGTTTTTVTTTTADSTTSGSATTTSGAATTTSGSAETTSATTGTDNTGETTTTTKGQLVPLYGDVNVNGQVTIVDVVLLNKAIAGKVTLSEQAFLNADCGNVDQVLDEHDLNALMQYLVGIVQQLPGEAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVL3</accession>
    <name>R5VVL3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">50S ribosomal protein L18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rplR</name>
        <name evidence="9" type="ORF">BN566_00073</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">This is one of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Part of the 50S ribosomal subunit; part of the 5S rRNA/L5/L18/L25 subcomplex. Contacts the 5S and 23S rRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the universal ribosomal protein uL18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000241">
        <property type="protein sequence ID" value="CCZ84527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00073"/>
    <dbReference type="EnsemblBacteria" id="CCZ84527">
        <property type="protein sequence ID" value="CCZ84527"/>
        <property type="gene ID" value="BN566_00073"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01337_B">
        <property type="entry name" value="Ribosomal_L18_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005484">
        <property type="entry name" value="Ribosomal_L18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004389">
        <property type="entry name" value="Ribosomal_L18_bac-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12899">
        <property type="entry name" value="PTHR12899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00861">
        <property type="entry name" value="Ribosomal_L18p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00060">
        <property type="entry name" value="L18_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7 9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 7" id="KW-0699">rRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ84527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="119" mass="13058" checksum="BFFDC455C59D1637" modified="2013-07-24" version="1">MIKKADSNKARAKRHARVRAKISGTPERPRLNVYRSTKHIYAQLIDDVNGVTLASASSMDKDFEGVGGNTEAARKVGTKIAERALAKNITEVVFDRGGYLYHGRVKELAEGAREGGLKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVN0</accession>
    <name>R5VVN0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphoribosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00418</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000244">
        <property type="protein sequence ID" value="CCZ84557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00418"/>
    <dbReference type="EnsemblBacteria" id="CCZ84557">
        <property type="protein sequence ID" value="CCZ84557"/>
        <property type="gene ID" value="BN566_00418"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pribosyltran" evidence="1">
        <location>
            <begin position="45"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="182" mass="19741" checksum="1F4384114C97EC89" modified="2013-07-24" version="1">MAEYYTLQVAGLTRKLKRCRISDKLEIAAFILFSDVELTVACAKELAEKCADCNYDYIMTAESKGIPLAYELSRQTGKPYVVARKSIKGYMTDCISVSVHSITTAAPQTLYLDGEKAAWFQGKRILLVDDVISTGESIAALEALTREAGGIVAGKATVLAEGDAIDRPDIIALAPLPLFEVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVQ5</accession>
    <name>R5VVQ5_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN566_00091</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CBAR010000252">
        <property type="protein sequence ID" value="CCZ84597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00091"/>
    <dbReference type="EnsemblBacteria" id="CCZ84597">
        <property type="protein sequence ID" value="CCZ84597"/>
        <property type="gene ID" value="BN566_00091"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="178"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="9">
        <location>
            <begin position="202"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="8">
        <location>
            <begin position="262"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ84597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="478" mass="53207" checksum="41996B13903A206C" modified="2013-07-24" version="1">MTADATQKKRRSSRKNTITVRWLISNLGVVFLVLLIVELSVIYTLQHYYYNSAEQYLTTKISSVTTVLTRYSQDTGSNFSADLRSTFENFSDKERMELMAINSKGRVTLTTSGFAPDTGTAMPDYETAMESETGTWTGRQNGERVMAVSVNISAMQPEYNAIRVVSSMSQIDATLRDYTTMITIVCIGVLLLLTITGSFFLRSIVRPINRINAVTKKFAQGDFSQRITPIPQDEIGDLCKAVNDMASALSETEAMKNEFISSVSHELRTPLTAIQGWAETLCLDIDAETMQKGMHVIVNETERLSRMVEELLDFSRMQSGRLTVQPEPMDILAELGDAVLIYMEKAKREHIRMIYQEPEMLPFVYGDKNRIRQVFINVIDNALKYSNPGGLVTVSAEAVAEQIQVRISDNGVGISAADLPKVKTKFFKANHTRRGSGIGLAVANEIITMHHGKLDIQSELGKGTTVIITLPAMSDHLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5UWS3</accession>
    <name>R5UWS3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00578</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000007">
        <property type="protein sequence ID" value="CCZ82773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00578"/>
    <dbReference type="EnsemblBacteria" id="CCZ82773">
        <property type="protein sequence ID" value="CCZ82773"/>
        <property type="gene ID" value="BN566_00578"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="171" mass="19028" checksum="5B6210B49B75A769" modified="2013-07-24" version="1">MAIAIRAYQPEDVPALVEIWNEVVEQGIAFPQKAPLTNQIGAAFFAKQSCTAAAYDTETAEIVGMYILHPNNVGRCGHICNASYAVKTTYRGQKIGEQLVRHCLHQARQMGFRILQFNAVVKENHAARKLYEKLGFVTIGTVAEGFQKKDGTYSDIVLYYFPLQNEPTAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5UX36</accession>
    <name>R5UX36_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Precorrin-2 C(20)-methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN566_00675</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the precorrin methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000020">
        <property type="protein sequence ID" value="CCZ82873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00675"/>
    <dbReference type="EnsemblBacteria" id="CCZ82873">
        <property type="protein sequence ID" value="CCZ82873"/>
        <property type="gene ID" value="BN566_00675"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030788">
        <property type="term" value="F:precorrin-2 C20-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11645">
        <property type="entry name" value="Precorrin_2_C20_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012382">
        <property type="entry name" value="CobI/CbiL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006364">
        <property type="entry name" value="CobI/CbiL/CobIJ_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036427">
        <property type="entry name" value="Precrrn-2_mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="SSF53790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01467">
        <property type="entry name" value="cobI_cbiL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="4 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TP_methylase" evidence="8">
        <location>
            <begin position="6"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR036427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ82873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="232" mass="25300" checksum="9668524E49E74C88" modified="2013-07-24" version="1">MKTGIFYAVGVGTEPLDLTLRAKQVLETAEVLVTPITRAGEPSAAARIAEQVVSFSGKEIIPLLFPMQKHLDYRERLRGELLQPIRDCLHAGKRVAMVTLGDVSIYSTAAYVQQVLEADGYATEVVAGISAFSAGAAKAKCSLCERQESLLVLPGGAAPETIQQALEQFDTLVLMKAGKALPWLVPLLQERQLLEHTTMLQNIGMEGEWIGTPTAEPTSYFTTLIIRHPRNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXE2</accession>
    <name>R5UXE2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00768</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000032">
        <property type="protein sequence ID" value="CCZ82968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82968">
        <property type="protein sequence ID" value="CCZ82968"/>
        <property type="gene ID" value="BN566_00768"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="190" mass="22203" checksum="EFFB2BD2B5A8A932" modified="2013-07-24" version="1">MFSIFWNLLAVNQNAMVSAGDKPIYLCYSILFCRYINDINLQNTCVRFFTNEICRCMEDGDVRQHLKNAEFKKLYQDIKNTYFWNKDDEKKEIYQAAKNTGDVNLMEIFRVRKSTSNVASTRKEQSKVRHKIIFLVSISMIIIIISLLGVALRINSSEKATMIMTGLLILYIIVALWILVPLLLKKYRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXQ2</accession>
    <name>R5UXQ2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00660</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000012">
        <property type="protein sequence ID" value="CCZ82855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82855">
        <property type="protein sequence ID" value="CCZ82855"/>
        <property type="gene ID" value="BN566_00660"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ82855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="360" mass="41727" checksum="1E7BD050B9798479" modified="2013-07-24" version="1">MTTETLHFLKALYANETRQVWVFDETNTCIWKNDISKPLQSEADCQALLKTVAANRGSQLFWHESLLYAAEVQQSTELHCTILKITAEPVLTNVMKEKNFRAICQEFLANQREAVFQISTIAEQIYDEVESNNIGDLEQEAVFSNLNDVMRACCHLLRRTNYYAELEKYAESESMALQPIELSRLLFDFSQNCKETLGRRISLKIQADTKIWVLCNEARLCFSLLCLMTQLLAQGQSELTRTVFLYVFSEGNTATVSIQMEKPECDFPEIEPTVLFQSNEEVAKQDYNLTNAVLENFCKTYNAKLERTSTDTKQGYLLKIPVYNARSNLQLHANIPRRRRSTFLNNYQVMLYDISDYRFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXT7</accession>
    <name>R5UXT7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00692</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000022">
        <property type="protein sequence ID" value="CCZ82890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82890">
        <property type="protein sequence ID" value="CCZ82890"/>
        <property type="gene ID" value="BN566_00692"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ82890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="167" mass="19122" checksum="7DEE9604E34664BE" modified="2013-07-24" version="1">MKCNQDLFETLFQTAQKPLVMECSQALEAIRFSLPIDWTVQQQSESLLYLLPSEGESSLSVQSVVPESTDIMDFESVSVLHAFADGMREQLENYAYTELCDYEGVFDAEQPIYGMRYTVTVNDVEMTTVTLLFLVEHRIYCLSFSDALQTELFPYAKSFLENLMISE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXU7</accession>
    <name>R5UXU7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Small acid-soluble spore proteins alpha/beta type</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00702</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000022">
        <property type="protein sequence ID" value="CCZ82900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00702"/>
    <dbReference type="EnsemblBacteria" id="CCZ82900">
        <property type="protein sequence ID" value="CCZ82900"/>
        <property type="gene ID" value="BN566_00702"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.10.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001448">
        <property type="entry name" value="SASP_alpha/beta-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038300">
        <property type="entry name" value="SASP_sf_alpha/beta"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00269">
        <property type="entry name" value="SASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ82900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="59" mass="6532" checksum="13E2A12A70C2932E" modified="2013-07-24" version="1">MKGEFTQKGKEAMERFKVESANEVGVNLKQGYNGDLTARQAGSIGGMMVKKMIDAYKMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXW4</accession>
    <name>R5UXW4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00946</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000069">
        <property type="protein sequence ID" value="CCZ83163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83163">
        <property type="protein sequence ID" value="CCZ83163"/>
        <property type="gene ID" value="BN566_00946"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="73" mass="8139" checksum="0BE989C3F425D6AB" modified="2013-07-24" version="1">MAEPKNTGKGKGMIGGIVIAVILFLLIAWRRQRTVRNFFHLFQESPVTAIVLLLIAVAVVAGIALFRKYREKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYA9</accession>
    <name>R5UYA9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">2,3-bisphosphoglycerate-independent phosphoglycerate mutase</fullName>
            <shortName evidence="8">BPG-independent PGAM</shortName>
            <shortName evidence="8">Phosphoglyceromutase</shortName>
            <shortName evidence="8">iPGM</shortName>
            <ecNumber evidence="8">5.4.2.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">gpmI</name>
        <name evidence="14" type="ORF">BN566_00798</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>(2R)-2-phosphoglycerate = (2R)-3-phosphoglycerate</text>
            <dbReference type="Rhea" id="RHEA:15901"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="EC" id="5.4.2.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="8">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 3/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the BPG-independent phosphoglycerate mutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.12" evidence="8"/>
    <dbReference type="EMBL" id="CBAR010000038">
        <property type="protein sequence ID" value="CCZ83005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00798"/>
    <dbReference type="EnsemblBacteria" id="CCZ83005">
        <property type="protein sequence ID" value="CCZ83005"/>
        <property type="gene ID" value="BN566_00798"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00186"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046537">
        <property type="term" value="F:2,3-bisphosphoglycerate-independent phosphoglycerate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006007">
        <property type="term" value="P:glucose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16010">
        <property type="entry name" value="iPGM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1450.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01038">
        <property type="entry name" value="GpmI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011258">
        <property type="entry name" value="BPG-indep_PGM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006124">
        <property type="entry name" value="Metalloenzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036646">
        <property type="entry name" value="PGAM_B_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005995">
        <property type="entry name" value="Pgm_bpd_ind"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31637">
        <property type="entry name" value="PTHR31637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06415">
        <property type="entry name" value="iPGM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01676">
        <property type="entry name" value="Metalloenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001492">
        <property type="entry name" value="IPGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="SSF53649"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64158">
        <property type="entry name" value="SSF64158"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01307">
        <property type="entry name" value="pgm_bpd_ind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6 8 11" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 8 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metalloenzyme" evidence="12">
        <location>
            <begin position="4"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="iPGM_N" evidence="13">
        <location>
            <begin position="81"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8 10">
        <location>
            <begin position="152"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8 10">
        <location>
            <begin position="255"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphoserine intermediate" evidence="8 9">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8 11">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 11">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 11">
        <location>
            <position position="397"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 11">
        <location>
            <position position="401"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 11">
        <location>
            <position position="438"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 11">
        <location>
            <position position="439"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 11">
        <location>
            <position position="456"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001492-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001492-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001492-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF06415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CCZ83005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="506" mass="55450" checksum="22922F81AC615812" modified="2013-07-24" version="1">MSKKPVALIIMDGFGYNKDTFGNAIAAANKPNIDKYLQGPHTLIGASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQMLVKITKDIQDGVFFENKALCDAMENCKKNGTALHLMGLLSPGGVHSHMEHLFGLLEMAKRHGLKDVYVHAFLDGRDEPPTSAAGFMKTTCEKMQEIGVGKIATISGRYYAMDRDNAWDRVEKAYAAMVYGEGETATEPVQAIEDSYAKEVTDEFMLPTVLDQNGLIKEQDSVIFFNFRPDRARQITRSFVDPEFKGFARKKGFFPLHFVCMAQYDATMPNVTVAYPPEQLHMTLGEYLSKCGKTQLRIAETQKYAHVTFFFNGGEEKVFDGEERILIPSPDVPTFDLKPEMSAYEVTDAVVKVIEEEKYDVIILNYANCDMVGHTGIFDAAKQAVEAVDTCVGRMVDAILAKGGVALITADHGNADKMCEPDGTPFTAHTTNPVPFIVVGEDCKLRDGGVLADIAPTMLQVLGLPQPEEMTGKSLIVSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYM5</accession>
    <name>R5UYM5_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptide deformylase</fullName>
            <shortName evidence="2">PDF</shortName>
            <ecNumber evidence="2">3.5.1.88</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Polypeptide deformylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">def</name>
        <name evidence="3" type="ORF">BN566_00922</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>H2O + N-terminal N-formyl-L-methionyl-[peptide] = formate + N-terminal L-methionyl-[peptide]</text>
            <dbReference type="Rhea" id="RHEA:24420"/>
            <dbReference type="Rhea" id="RHEA-COMP:10639"/>
            <dbReference type="Rhea" id="RHEA-COMP:10640"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:49298"/>
            <dbReference type="ChEBI" id="CHEBI:64731"/>
            <dbReference type="EC" id="3.5.1.88"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="2">Binds 1 Fe(2+) ion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the polypeptide deformylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.88" evidence="2"/>
    <dbReference type="EMBL" id="CBAR010000062">
        <property type="protein sequence ID" value="CCZ83135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00922"/>
    <dbReference type="EnsemblBacteria" id="CCZ83135">
        <property type="protein sequence ID" value="CCZ83135"/>
        <property type="gene ID" value="BN566_00922"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042586">
        <property type="term" value="F:peptide deformylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00487">
        <property type="entry name" value="Pep_deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.45.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00163">
        <property type="entry name" value="Pep_deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023635">
        <property type="entry name" value="Peptide_deformylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036821">
        <property type="entry name" value="Peptide_deformylase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10458">
        <property type="entry name" value="PTHR10458"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01327">
        <property type="entry name" value="Pep_deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004749">
        <property type="entry name" value="Pep_def"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01576">
        <property type="entry name" value="PDEFORMYLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56420">
        <property type="entry name" value="SSF56420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="2">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="137" mass="15685" checksum="8F821586AA60900F" modified="2013-07-24" version="1">MIREIVTDPFFLSQKSEPATKQDQQVIQDLLDTIHAHADHCVGMAANMIGVRKTILVAQIGATYQILINPVIVDHSKQSYETEEGCLSLTGQRSTQRYPMIVVEYLDRNFKKKKQIFRDFEAQVIQHEIDHFSGILI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYN3</accession>
    <name>R5UYN3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Carbohydrate ABC transporter membrane protein 2 CUT1 family (TC 3.A.1.1.-)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_01197</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000102">
        <property type="protein sequence ID" value="CCZ83423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01197"/>
    <dbReference type="EnsemblBacteria" id="CCZ83423">
        <property type="protein sequence ID" value="CCZ83423"/>
        <property type="gene ID" value="BN566_01197"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="122"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="254"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="84"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ83423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="290" mass="32525" checksum="8321687654DE4667" modified="2013-07-24" version="1">MRAASNPVRLGKYSLGKIFFTICKYVLLIFAAFCALVPLVSCVITAFKTKAEYNNTNVMTLPESWLYFDNFVTAWKQANMGKAFLNSFLVLLFVLLGSTLFSAMLAYVLNRFQFRGNKLIRNLFMISTLIPGIATQVTIYQIMYTLGLVNTLYGYIILMLGTDVISIYIFLQYFDNLPKTLDEAAILDGCTYVGVFFKILLPLLKPAIVTSAILKGVSTYNEYYMANLYLQDKKAFPVVSTSLFTFTGPMGSQYNYICAGVLITVIPMLILFICCQKQIYSGMAQGAVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYT7</accession>
    <name>R5UYT7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator TetR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00988</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000070">
        <property type="protein sequence ID" value="CCZ83205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83205">
        <property type="protein sequence ID" value="CCZ83205"/>
        <property type="gene ID" value="BN566_00988"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="8"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="231" mass="26434" checksum="77B6F342237D5A06" modified="2013-07-24" version="1">MAKRYDSRAAKMKIMEACVPLFLEKGYYDTTIKDILTAANVAHSSFFNIFPTKDALLLEYVKYMFQNQFDMANQFLGDKAFPALIYAVETSIQLAVTEMKETLRSIYVEAYSAEGSLNYIIHHTAMEVQKLFGQYFPEANSESDFYERVIGSSGMMRGYMVIPCDLYFTLEKKIQRFLEMSLTSYRVPLEEQKKAIGIVLQMDLRTIAEGAIQSLTAKLRSHFTVDPAIAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYY2</accession>
    <name>R5UYY2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Bacterial group 2 Ig-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01036</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01036"/>
    <dbReference type="EnsemblBacteria" id="CCZ83255">
        <property type="protein sequence ID" value="CCZ83255"/>
        <property type="gene ID" value="BN566_01036"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029221">
        <property type="entry name" value="PBP-Tp47_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029218">
        <property type="entry name" value="PBP-Tp47_dom_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038698">
        <property type="entry name" value="PBP_Tp47_domC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038031">
        <property type="entry name" value="Tp47_mid_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036154">
        <property type="entry name" value="Tp47_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14889">
        <property type="entry name" value="PBP-Tp47_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14888">
        <property type="entry name" value="PBP-Tp47_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81986">
        <property type="entry name" value="SSF81986"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82220">
        <property type="entry name" value="SSF82220"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004394647" evidence="1">
        <location>
            <begin position="32"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="2">
        <location>
            <begin position="789"/>
            <end position="864"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="867" mass="93318" checksum="71FAC8EE4DFE3C92" modified="2013-07-24" version="1">MRKHTQIRIASAAMATLLAATQIAMPFPAVAADEDYVYCAAALSWAEYWEQEGVFLSDDANGDWTAESADSDTRGESDLGAYDTVTRATSNHGLHRGSYQCTVKVYDTDGNAYDYTGKGNGESVDVVKGDNGTMITVDGKEVGTYDHSMILGPKYVPVAVPASLYDDFKQKYSVLENGDSMVGGYTEKNLVAINETVDVSANTDGLKTVSQNEDGTYTFSKRAETNVGKTVDESAIEISDVKYKSGYGDFLRVDIGGDYGDLGAHMYAVRWDYYGDQDTVLASYGTKFAADNWMHKSMGIQLGLTKSLRCQLPTGYESGAGKWNVTVYATGYQDYTFTIEAAPTEYVYGTMDIPYADYYYGELGNKTNTTDGTISYTSDLAGDAGMRAENTYDAVTSATAKKWTKQAGTYTSEADENGGGKILGVKGVEVAIEKNLYNSLLADKESGKTSGTLGSLFDFFTVNEDQNEPQAYKELYADGTLSATISQKAEVDLSNVTPSISTDTKYGDYEIDMDGLDLGDDTVYGAVVTMKDGKQYGMLHLENIWKGGKELAWSVGVKTQESHGNTLRYTPYVETSGATIAEVTYLTNTGIYSVHSADGLYLPKKHTATITAEDTDVTAGTANVTITDLPEDFGLSVAVANLEGAAYADGKLTYDAAAAKPGKYSVDVTDSNNVYAHFTTNLLLTTEAIPVAYNAETNKLTITDDSTDADLTNYVSNLATVTVNDTPYSASGRGAVTIVKEDGTVDFSAAKSFRGETTEIFPTAGEYTLKLTANGYVNDYTFTVTVPEKTIEKGDVDGDQSISVSDAVVVLTYYAKKAAGQDVSQDEIFQNILVGDVNDDGEITVEDAVAILTYYAKKAAGQDATWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZ11</accession>
    <name>R5UZ11_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Acetyltransferase GNAT family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01071</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01071"/>
    <dbReference type="EnsemblBacteria" id="CCZ83290">
        <property type="protein sequence ID" value="CCZ83290"/>
        <property type="gene ID" value="BN566_01071"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="9"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="172" mass="19702" checksum="B8D19AAE27DD6E73" modified="2013-07-24" version="1">MPVIQTERLVLRPLTTNDFSAFRQFAADAELTRYMLFYPQETESETKAFLQHAEQAWTEQCPEAYEFAILQEDTLIGTITVEYMGDTQGMLGWILRKDAQGYGYATEAALAIVQWVKEQLPYTELFACCDARNPASARVMERIGMHCIDANGSRTYPKTGEVAQEWMYQMNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZ31</accession>
    <name>R5UZ31_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cell division protein SepF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">sepF</name>
        <name evidence="7" type="ORF">BN566_01336</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Cell division protein that is part of the divisome complex and is recruited early to the Z-ring. Probably stimulates Z-ring formation, perhaps through the cross-linking of FtsZ protofilaments. Its function overlaps with FtsA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer. Interacts with FtsZ.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="5">Localizes to the division site, in a FtsZ-dependent manner.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SepF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000131">
        <property type="protein sequence ID" value="CCZ83568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01336"/>
    <dbReference type="EnsemblBacteria" id="CCZ83568">
        <property type="protein sequence ID" value="CCZ83568"/>
        <property type="gene ID" value="BN566_01336"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000917">
        <property type="term" value="P:division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043093">
        <property type="term" value="P:FtsZ-dependent cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01197">
        <property type="entry name" value="SepF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023052">
        <property type="entry name" value="Cell_div_SepF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007561">
        <property type="entry name" value="Cell_div_SepF/SepF-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038594">
        <property type="entry name" value="SepF-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35798">
        <property type="entry name" value="PTHR35798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04472">
        <property type="entry name" value="SepF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 5 7" id="KW-0132">Cell division</keyword>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0717">Septation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="38"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ83568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="176" mass="19193" checksum="2D91DAFEE160041E" modified="2013-07-24" version="1">MSFFNGLKDFFMPNEDEYAEPTEPVTKKEPEQPAAAAPSPQPSSSYESSSSAYSSASSYTPPVNPAPAPNEPPRNNRVVNIKATTQLQVVLVKPELFTDAKQIADHLMANKTVVLNLESATEANRRRIIDFLVGVAYAQNGNIKPVANQTYIVTPYNVGFVGDEFVGELENNGLIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZ52</accession>
    <name>R5UZ52_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">HPr kinase/phosphorylase</fullName>
            <shortName evidence="10">HPrK/P</shortName>
            <ecNumber evidence="10">2.7.11.-</ecNumber>
            <ecNumber evidence="10">2.7.4.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">HPr(Ser) kinase/phosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">hprK</name>
        <name evidence="13" type="ORF">BN566_01361</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the ATP- as well as the pyrophosphate-dependent phosphorylation of a specific serine residue in HPr, a phosphocarrier protein of the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS). HprK/P also catalyzes the pyrophosphate-producing, inorganic phosphate-dependent dephosphorylation (phosphorolysis) of seryl-phosphorylated HPr (P-Ser-HPr). The two antagonistic activities of HprK/P are regulated by several intracellular metabolites, which change their concentration in response to the absence or presence of rapidly metabolisable carbon sources (glucose, fructose, etc.) in the growth medium. Therefore, by controlling the phosphorylation state of HPr, HPrK/P is a sensor enzyme that plays a major role in the regulation of carbon metabolism and sugar transport: it mediates carbon catabolite repression (CCR), and regulates PTS-catalyzed carbohydrate uptake and inducer exclusion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>[HPr protein]-L-serine + ATP = [HPr protein]-O-phospho-L-serine + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46600"/>
            <dbReference type="Rhea" id="RHEA-COMP:11602"/>
            <dbReference type="Rhea" id="RHEA-COMP:11603"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 10">
            <text>[HPr protein]-O-phospho-L-serine + H(+) + phosphate = [HPr protein]-L-serine + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:46604"/>
            <dbReference type="Rhea" id="RHEA-COMP:11602"/>
            <dbReference type="Rhea" id="RHEA-COMP:11603"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homohexamer.</text>
    </comment>
    <comment type="domain">
        <text evidence="10">The Walker A ATP-binding motif also binds Pi and PPi.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="10">Both phosphorylation and phosphorolysis are carried out by the same active site and suggest a common mechanism for both reactions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the HPrK/P family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.-" evidence="10"/>
    <dbReference type="EC" id="2.7.4.-" evidence="10"/>
    <dbReference type="EMBL" id="CBAR010000144">
        <property type="protein sequence ID" value="CCZ83598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01361"/>
    <dbReference type="EnsemblBacteria" id="CCZ83598">
        <property type="protein sequence ID" value="CCZ83598"/>
        <property type="gene ID" value="BN566_01361"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006109">
        <property type="term" value="P:regulation of carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01918">
        <property type="entry name" value="HprK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1390.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01249">
        <property type="entry name" value="HPr_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003755">
        <property type="entry name" value="HPr(Ser)_kin/Pase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011104">
        <property type="entry name" value="Hpr_kin/Pase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011126">
        <property type="entry name" value="Hpr_kin/Pase_Hpr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028979">
        <property type="entry name" value="Ser_kin/Pase_Hpr-like_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30305:SF1">
        <property type="entry name" value="PTHR30305:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07475">
        <property type="entry name" value="Hpr_kinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02603">
        <property type="entry name" value="Hpr_kinase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75138">
        <property type="entry name" value="SSF75138"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00679">
        <property type="entry name" value="hpr-ser"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7 10 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 10" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Hpr_kinase_N" evidence="11">
        <location>
            <begin position="6"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Hpr_kinase_C" evidence="12">
        <location>
            <begin position="133"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Important for the catalytic mechanism of both phosphorylation and dephosphorylation" evidence="10">
        <location>
            <begin position="204"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Important for the catalytic mechanism of dephosphorylation" evidence="10">
        <location>
            <begin position="267"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="141"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="162"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for phosphorylation activity. Proton donor; for dephosphorylation activity" evidence="10">
        <location>
            <position position="180"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="246"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="156"/>
            <end position="163"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF07475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CCZ83598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="318" mass="35869" checksum="FFA4643BC51D903E" modified="2013-07-24" version="1">MAKFTISLDKIIDEFKLETIYTPKNASEIEIDEADVTRPGLQLMGFYEYFNPERIQIIGKMEYAYLSTIDEETRVQRLDMLFSQRIPALVISRELPYFEEMLDLAKKYSVPLLRSKESTSNFIAGLIAFLNLNLAPRITRHGVLIEIYGEGVFITGESGVGKSETAIELVKRGHRLVADDAVEIRKVSNISLVGSSPDNIRHFLELRGIGIINARRLFGIGAVKNTEKIELIVEMEQWNPEKIYDRMGVDTQFVSLLGVKVPSLTIPVRPGRNLAVILEVAAMNNRQKKMGYNAASDLLDHLGLEAEAKEVIKDYDAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZE2</accession>
    <name>R5UZE2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Efflux ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN566_01171</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000094">
        <property type="protein sequence ID" value="CCZ83395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01171"/>
    <dbReference type="EnsemblBacteria" id="CCZ83395">
        <property type="protein sequence ID" value="CCZ83395"/>
        <property type="gene ID" value="BN566_01171"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025857">
        <property type="entry name" value="MacB_PCD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12704">
        <property type="entry name" value="MacB_PCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="296"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="341"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="383"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="MacB_PCD" evidence="8">
        <location>
            <begin position="19"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX" evidence="7">
        <location>
            <begin position="325"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ83395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="419" mass="44813" checksum="BEC6589A952F41B7" modified="2013-07-24" version="1">MLENIRLSIQGIFAHKIRSILTMLGIIIGIAAIIAIVSTIRGTNEQIKSNLVGAGNRTVTVSLYQGEYELDLQYQGLPSGVPVISSQVKAQLSAIEDVSAVSLYRTRSYCDSIFYQNTKLEGGSVWGIDADYFQTAGYHVQSGKGFRDADYTNGNNVAILDEDAAQSLFAGANPIGSVIDINGMPFKIIGTVVQSSTFEPVIQSIDDYHLYANSTVGKVFLPDSAWPTAFQYDEPQNCLLKATETDAMTTVGKQAADILNGRISVSGTGETIQYKSKDLLEQAKSLQELSSSTNTMLIYIAGISLLVGGIGVMNIMLVSVTERTREIGLKKALGAKKRNILFQFLTEAAVLTSVGGLLGVIAGIGLSRIIAKLAEVPVSISPAAAIIAVLFSMVVGILFGWIPSVKAANLNPIDALRYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZJ8</accession>
    <name>R5UZJ8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">1-deoxy-D-xylulose-5-phosphate synthase</fullName>
            <ecNumber evidence="10">2.2.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">1-deoxyxylulose-5-phosphate synthase</fullName>
            <shortName evidence="10">DXP synthase</shortName>
            <shortName evidence="10">DXPS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">dxs</name>
        <name evidence="12" type="ORF">BN566_01214</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>D-glyceraldehyde 3-phosphate + H(+) + pyruvate = 1-deoxy-D-xylulose 5-phosphate + CO2</text>
            <dbReference type="Rhea" id="RHEA:12605"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57792"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="2.2.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="10">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
        <text evidence="10">Binds 1 thiamine pyrophosphate per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 10">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the transketolase family. DXPS subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.7" evidence="10"/>
    <dbReference type="EMBL" id="CBAR010000105">
        <property type="protein sequence ID" value="CCZ83440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01214"/>
    <dbReference type="EnsemblBacteria" id="CCZ83440">
        <property type="protein sequence ID" value="CCZ83440"/>
        <property type="gene ID" value="BN566_01214"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00064">
        <property type="reaction ID" value="UER00091"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008661">
        <property type="term" value="F:1-deoxy-D-xylulose-5-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052865">
        <property type="term" value="P:1-deoxy-D-xylulose 5-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02007">
        <property type="entry name" value="TPP_DXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00315">
        <property type="entry name" value="DXP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005477">
        <property type="entry name" value="Dxylulose-5-P_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005474">
        <property type="entry name" value="Transketolase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43322">
        <property type="entry name" value="PTHR43322"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13292">
        <property type="entry name" value="DXP_synthase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00204">
        <property type="entry name" value="dxs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00801">
        <property type="entry name" value="TRANSKETOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="6 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="8 10" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TRANSKETOLASE_1" evidence="11">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Thiamine pyrophosphate binding" evidence="10">
        <location>
            <begin position="124"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Thiamine pyrophosphate binding" evidence="10">
        <location>
            <begin position="156"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="184"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="376"/>
        </location>
        <ligand>
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS00801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CCZ83440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="635" mass="69961" checksum="F19642308DCCF479" modified="2013-07-24" version="1">MNENNIPAEKRFSLATMQLPEDLKKLNTEQCQQLCKEIRSVLIQTVSKNGGHLASNLGVVELTVAMHRVFDSPQDKFVWDVGHQCYTHKILTGRYAQFSTLRKENGISGFPKPEESPHDTFISGHSSTAISVASGVAEAMRQNGDPHHVIAVLGDGAMTGGLAFEGLNNAGKSKNRLIIILNDNAMSISKNVGSVAKYLANIRNSENYVKTKKAVERKLQKTPVIGAPVAKMIKSSKDALRDTVFRSATIFEDFGFVYLGPVDGHNLEDLEEVLQAAKAYECPVFVHIHTKKGKGYLPSEKNPGEFHGISRFNVETGNPEISGKDTYSDIFGKELVRLAKKDASICAITAAMEHGTGLQYFSRAYPQRYYDVGIAEQHAVTFAAALASQGKLPVFAVYSSFLQRAYDQLLHDVAIGKLHVVLGIDRAGVVGEDGETHHGLFDVSFLTSIPGTTIYAPACYDELTLCLRQAMYQDTGLACARYPRGADNTIFDKSALNTAYTHTAGCRTDTLLIGYGRTYDLLYRAKQAAEKEGFSCDLLKLTQIFPLPELVPSICRSYQRILFFEEAYYYGGISQLLGDLLLEMGYTGSYQRIAPKQFIPQASIAAQLDEIGLSEAAMLKKIRQTVEQKEKEQSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZU7</accession>
    <name>R5UZU7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01607</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000169">
        <property type="protein sequence ID" value="CCZ83878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01607"/>
    <dbReference type="EnsemblBacteria" id="CCZ83878">
        <property type="protein sequence ID" value="CCZ83878"/>
        <property type="gene ID" value="BN566_01607"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015231">
        <property type="entry name" value="DUF1934"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09148">
        <property type="entry name" value="DUF1934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="SSF50814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="143" mass="16262" checksum="47F40A9C93203DA7" modified="2013-07-24" version="1">MKKKVEIILRSVQRNEEEENETELRTEGVYEHDGKNWSVSYEESEATGFEDSTTEITGTEGRCVSIVRRGSANSNLMIETGKKHYCLYGTPFGDMTLGIYTNKIEDNLSPQGGRLYLRYTLDVNASFLSDNEIYLEVVTQQEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZV6</accession>
    <name>R5UZV6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01617</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000169">
        <property type="protein sequence ID" value="CCZ83888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01617"/>
    <dbReference type="EnsemblBacteria" id="CCZ83888">
        <property type="protein sequence ID" value="CCZ83888"/>
        <property type="gene ID" value="BN566_01617"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025699">
        <property type="entry name" value="ABC2_memb-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13346">
        <property type="entry name" value="ABC2_membrane_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="208" mass="23172" checksum="8B89575B0CECD495" modified="2013-07-24" version="1">MLGILWKDLKVMRMQTAILTSVFLMMTVASVWNTGRQDDISFFFNFSTLMPIFFTLLPCSALALEQQEQTNAWLMTLPCSRKQMVLEKYVLLLVFGLGSAAVVGIVTAILTGNALSFALAMRSGILGICFQCVLLPLYYKLGFQKAKFIYMLLCVLVGGVTGFLSGSGSTWLTSLHLSITLWICSIVCLVLLPVSMRFSVQWMAEKEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>R5V052</accession>
    <name>R5V052_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Endoglucanase</fullName>
            <ecNumber evidence="6">3.2.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN566_00152</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Endohydrolysis of (1-&gt;4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the glycosyl hydrolase 9 (cellulase E) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.4" evidence="6"/>
    <dbReference type="EMBL" id="CBAR010000146">
        <property type="protein sequence ID" value="CCZ83625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00152"/>
    <dbReference type="EnsemblBacteria" id="CCZ83625">
        <property type="protein sequence ID" value="CCZ83625"/>
        <property type="gene ID" value="BN566_00152"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008810">
        <property type="term" value="F:cellulase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030248">
        <property type="term" value="F:cellulose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008965">
        <property type="entry name" value="CBM2/CBM3_carb-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001956">
        <property type="entry name" value="CBM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036966">
        <property type="entry name" value="CBM3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001701">
        <property type="entry name" value="Glyco_hydro_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033126">
        <property type="entry name" value="Glyco_hydro_9_Asp/Glu_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00942">
        <property type="entry name" value="CBM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00759">
        <property type="entry name" value="Glyco_hydro_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01067">
        <property type="entry name" value="CBM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="SSF48208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49384">
        <property type="entry name" value="SSF49384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51172">
        <property type="entry name" value="CBM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00698">
        <property type="entry name" value="GH9_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5 6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="3 5 6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1 5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 5 6" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005145532" description="Endoglucanase" evidence="6">
        <location>
            <begin position="37"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="domain" description="CBM3" evidence="8">
        <location>
            <begin position="504"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="9">
        <location>
            <begin position="740"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="668"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="673"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="461"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ83625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="815" mass="90362" checksum="FD836574EF4C949F" modified="2013-07-24" version="1">MGFLMKKTGIALLSAAVLAITSAASASSGLFGTAEAADTTNSTYNYGEALQKSMFFYEVQQCGELPDWNEVSWRDDCMVNDYIPGGWFDAGDHLKFTLTNAYAATMLGWGLLEYQDGVKEIGELTEYKNNLAWALDYVASCDLGDEIVYMIGDGAFDHVWWGSAEVYMRKFKLMKGEDERPYYTCNDSCIEGQMAAALAVGYLCFKDSDPDRADNYLAHAKACFERADKNRSIGDDTEEHKYYKPSSFYDDLFFAANWLYRATGEQSYLDLCKTDYIPNLGKEEQSSEMKYTWGHCWDDTMQGGMLLYAMNTGDSQWKEQFRKHLEYWTTGYGGKQIAHTPDGLAWLFQWGSMRHATTTAFLAYVAADELFADDAAAATKYTDFADSTMNYAFGDNDLDMSYVIGMGDKYPQAWHHRTSSGAWNDKWSNIGQTEGEDAKPHAHTLYGALVGGPDQTGKYSDKIGDYQYTEVAIDYNAGYTAALCAMVQKYGGSSDPDFPPTETPKWTEFFMRASINQAAGSYTEIKAFAMNHSAWPARVIQNLSYNYYFDISELVDAGYSIDDVSVRIGYDQHASDEGKMTISDPIHYKDNIYYVKLSFADGSVVMPTGQSEHRSECQFRISIPDNIQGVWDASNDYSFQGLTQGGEDAMVDTPYMTMYDGDTLIWGTEPDGTTPDDNPTTTTTTTTTTTTTTTTTTTVTTTAETTAETTAPATTTASSTTPTTEITTAVTTATSTIIDPDILYGDVDLDGTVSLTDAILLNKYLAGMVVLSDAALANANCYLDKDSNLNEDDTTYLMRFVLRAINDLPYIPIPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5V066</accession>
    <name>R5V066_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Cystathionine beta-lyase family protein involved in aluminum resistance</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01707</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000178">
        <property type="protein sequence ID" value="CCZ83978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01707"/>
    <dbReference type="EnsemblBacteria" id="CCZ83978">
        <property type="protein sequence ID" value="CCZ83978"/>
        <property type="gene ID" value="BN566_01707"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009651">
        <property type="entry name" value="Met_g_lyase_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46658">
        <property type="entry name" value="PTHR46658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06838">
        <property type="entry name" value="Met_gamma_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="429" mass="46247" checksum="347F7C4E7FEF4496" modified="2013-07-24" version="1">MMKEIQSFFEIDDRLCALAEQAEERCKPVFAAIDQRAAYNGAKVLKAFQNNRVSEPCFYGSTGYGYGDQGREVLDKVVAEAFGAEDALVRHNFVSGTHALTVALFGVLRTGDKMVSITGRPYDTLEGVIGLTGNKGDGSLMDYGVTYDEVPLLEDGTPNYDAIPEAVRGAKIAYIQRSRGYALRPAFTLAQIEKMAQLARSSNPDIIVMVDNCYGEFTDTSEPVQHGADLMIGSMIKNVGGGIARTGGYIAGRKDLVELCSYRLTCVGMGKEVGCTLDMNRELFLGFFHAPDVTASALKTAAFAASLFELLGFACYPRYDEPRNDIVEAICLGDEAHLTAFCQGIQAGSPVDSFVVPEAWDMPGYTSKVIMAAGAFTLGASIELSADAPIREPFAVWMQGGITETSGKIGVLLAAQKMLENQLLPTLQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0B6</accession>
    <name>R5V0B6_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Phosphoribosylformylglycinamidine cyclo-ligase</fullName>
            <ecNumber evidence="3 12">6.3.3.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 12">AIR synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11 12">AIRS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9 12">Phosphoribosyl-aminoimidazole synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">purM</name>
        <name evidence="15" type="ORF">BN566_01446</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 12">
            <text>2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + ATP = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:23032"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.3.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 12">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the AIR synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.3.1" evidence="3 12"/>
    <dbReference type="EMBL" id="CBAR010000154">
        <property type="protein sequence ID" value="CCZ83710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01446"/>
    <dbReference type="EnsemblBacteria" id="CCZ83710">
        <property type="protein sequence ID" value="CCZ83710"/>
        <property type="gene ID" value="BN566_01446"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00129"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004641">
        <property type="term" value="F:phosphoribosylformylglycinamidine cyclo-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02196">
        <property type="entry name" value="PurM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00741">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004733">
        <property type="entry name" value="PurM_cligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10520">
        <property type="entry name" value="PTHR10520"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="SSF55326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="SSF56042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00878">
        <property type="entry name" value="purM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12 15" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIRS" evidence="13">
        <location>
            <begin position="61"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="AIRS_C" evidence="14">
        <location>
            <begin position="176"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CCZ83710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="344" mass="36791" checksum="29331493C0FD4254" modified="2013-07-24" version="1">MKSYSESYKKAGVDVTAGYRAVDLMKKHIARTMTDNVLTGIGGFGGLYELDVTGMEHPVLVSGTDGVGTKLKIAFLMDRHNTIGIDCVAMCVNDIICCGAKPQFFLDYIALGKNVPEKVAEIVSGVAEGCVQAGCALVGGETAEMPGFYPVDEYDVAGFAVGLVDKKKVLDLNTQKEGDVLIALPSSGIHSNGYSLIRKVFTVNSQNLARHFSELGTTLGEALLMPTKIYVKPILSLLEQVTVKSIAHITGGGFYENIPRALKKGLSAQIEKKDVKVLPIFDLVAKTGNIPEHDMFNTFNMGVGMMVCVAQEDADKALETLRAAGEDAYVLGTLVSSDEGVILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0F8</accession>
    <name>R5V0F8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Hemagglutinin/hemolysin family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01794</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000186">
        <property type="protein sequence ID" value="CCZ84068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01794"/>
    <dbReference type="EnsemblBacteria" id="CCZ84068">
        <property type="protein sequence ID" value="CCZ84068"/>
        <property type="gene ID" value="BN566_01794"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="536" mass="61301" checksum="58F1545EE32CDE75" modified="2013-07-24" version="1">MTNSADWLEVYGNWNYITLTDMEGKWTAGNICFFGPIWEVNEASGSKSIYSSGSQVIHFGYEGGKQTVLWDNCETYINNEDGSLNTERTFNFNYKDETGKCLGVVFSPDYSNENYHFRPYLPQYVTNLEDAVPYKEWKYMEDSNGNTIPDIVEKQIAEDPLNVDNAWLEDKYGCSVEEFIKTDNVNADKNKKALVETLSYLYNHYIEPVADAASDYIGKSLNQIVKGNFTDDVTLLGTAGEIVLGIFDLDLVCDLRDITYDIGEWGEIIIGDDEFSWSFAGQSFLDLVGIIPVIGAVKYSDEVGALLKKAPNIYKNSVYKSVAECFTDGKSLKSEDEIAETVTNNLKKTQKQIEELLDDSGKFIDEEAESNYQKYVLRKQKSGKIPRDRIDWKEASDHWKYNSPMARGNEFNKIAEKIYPFNEVNLANGKRLDSYILGEKIISRKATDLSDIEFNTFDKYLSEMIQKYAPGTVIRSNKYKCLNGQILSGKMYLEIPAVNKDFDQIDEYMTYAKKKYDIKIIFLEENKVWDFSDLTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0N6</accession>
    <name>R5V0N6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01565</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000165">
        <property type="protein sequence ID" value="CCZ83830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83830">
        <property type="protein sequence ID" value="CCZ83830"/>
        <property type="gene ID" value="BN566_01565"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002793">
        <property type="entry name" value="Endonuclease_NucS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01939">
        <property type="entry name" value="NucS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="492" mass="57099" checksum="04DC8DD06E1DE915" modified="2013-07-24" version="1">MTEAALQELIKREFPAEMSVTEVADKLDTMQAHPDIEQIRIWFNNVLLAFITLDYLEYSENRKNFLKETSVQLFFRNTEKILGNPYFQAVACFFKGQYEKSLRLVRTVMKSNAEEEPYNLQELAYCYIVPFKNAFPGFWQMVVLQLKQSGAEQLVIEHAELVRDYYFAATEEEKCLLLTGFLQSHSDSVIAHELLGMTYGRMKRWKNQIACLETIEEQPCLFTKACINFQIGWAYGKEKDWEQEEAYYRNCLELEWDYPFANNNLAYSFYQQKRYAEAKELLEFCLEKELDLSCAANNYVRVLLAMGNGTAARSFIKSGAYPVSKSLVKRAKTAKNRQSIVLSAESAEQTKTGLQKKPLEKAEQFSSEWILEEELTARIEAGFPVFGKQLHLYRRNGEYGRQYVIPIGRLDLFCEDADGNLYVIELKKDGGYGDAYAQLAAYLDWFQKSKRFSKHSVSGILCVNHPSPQLLQKVHADARMRLYEYQISYTER</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0R4</accession>
    <name>R5V0R4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">50S ribosomal protein L10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rplJ</name>
        <name evidence="6" type="ORF">BN566_01595</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the ribosomal stalk of the 50S ribosomal subunit. The N-terminus interacts with L11 and the large rRNA to form the base of the stalk. The C-terminus forms an elongated spine to which L12 dimers bind in a sequential fashion forming a multimeric L10(L12)X complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000167">
        <property type="protein sequence ID" value="CCZ83860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01595"/>
    <dbReference type="EnsemblBacteria" id="CCZ83860">
        <property type="protein sequence ID" value="CCZ83860"/>
        <property type="gene ID" value="BN566_01595"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05797">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00362">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022973">
        <property type="entry name" value="Ribosomal_L10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043141">
        <property type="entry name" value="Ribosomal_L10-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_L10P"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11560">
        <property type="entry name" value="PTHR11560"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160369">
        <property type="entry name" value="SSF160369"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ83860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="171" mass="18437" checksum="94FACDA7F481494B" modified="2013-07-24" version="1">MASEKILESKKAKVAALTEKLQNSVSFVLVDYKGITVEEDTKLRKELREAGVSYTVEKNSMLRFALKEMGIVDSFEDVLHGTTAIAVSNDDQTAAARILGKFAESKPETFNLKAGYVDGVAYDTAGVMALSKIPSKDVLLAQLVGSLQGPLRKLAATLQALADKKNEEEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>R5V108</accession>
    <name>R5V108_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">FAD dependent oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN566_01679</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HdrA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000175">
        <property type="protein sequence ID" value="CCZ83950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01679"/>
    <dbReference type="EnsemblBacteria" id="CCZ83950">
        <property type="protein sequence ID" value="CCZ83950"/>
        <property type="gene ID" value="BN566_01679"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039650">
        <property type="entry name" value="HdrA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43498">
        <property type="entry name" value="PTHR43498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="234"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="282"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="582"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="625"/>
            <end position="654"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ83950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="668" mass="72085" checksum="4BD8E044E38D20BA" modified="2013-07-24" version="1">MQRIGVFVCHCGTNIASTVDVKTVAETLGKEPAVVFATDYPYMCSEAGQNIIKEAIKEHHLDGVVVCSCSPRMHEATFRKAVAAAGLNPYMLEVANIREQCSWIHKNKEEGTAKAIVLGRAAIAKVQLNAPLIAGESPVVKRALVIGGGIAGIQTALDIAEAGYPVDIVEKEPTIGGKMTQIDKTFPTLDCSSCILTPKMVECAQNENITIYSYSEIEAVTGFVGNFSVKIRRKKRYVDETKCTGCGICTEKCPVKNVPSAYDLGLKNHRAIYIPFAQAIPNVAVIDGDYCNKIQKGKCGVCARFCSAGAINYEQEDQIVEEKYGAIIAATGYNPISLDGYDEFAYSQSKDVVTSLEFERILKADGPTEGHLERLSDHKQPKTLVFVQCVGSRQSGDATHGGCGKEYCSKICCMYTAKHAMLCREKYPDTDVYVFYIDVRTPGKNYDEFYRRAVEQYGVHYIKGMVGKVSPQADGSLMVQASDLLSNEQLHIKADMVVLAAAIEPDKTARPLATMLTASMDTNDFFTEAHAKLRPVESPTAGVFLSGVCQGPKDIPETVSQASAAAAKALGLLAKDKLSCNPCVAHSNEMMCNGCSSCANVCPYGAITYSDKDFSRRKDGSMIRRVASVNEAVCQGCGACTVACPSGAMDLKGFSTSQIMAEVDAICK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1D9</accession>
    <name>R5V1D9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_01786</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000184">
        <property type="protein sequence ID" value="CCZ84060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01786"/>
    <dbReference type="EnsemblBacteria" id="CCZ84060">
        <property type="protein sequence ID" value="CCZ84060"/>
        <property type="gene ID" value="BN566_01786"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000522">
        <property type="entry name" value="ABC_transptr_permease_BtuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30472">
        <property type="entry name" value="PTHR30472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01032">
        <property type="entry name" value="FecCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81345">
        <property type="entry name" value="SSF81345"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="120"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="150"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="199"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="238"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="284"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="313"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ84060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="337" mass="36648" checksum="D6220E7F5A59F65D" modified="2013-07-24" version="1">MKSHSTIRYWICYLLLFVVLLALFVGTLCTGSVPFSLSEIGSVLMHPEQNTTAWKILWEIRMPRSLTAIILGGALSVSGFLLQTFFSNPIAGPYILGISSGAKLTVALAMIFFLGQGRLLSAMALIGVAFFGSMLSMGFILLISGKVHRMSILIICGVMIGYICSAITDFVVTFADDSNIVNLHNWSMGSFASSSWQDLRAMSMVVFPVLIGTFFLSKPMGAYQLGEAYAQNLGVPIRLFRVTLILLSSLLSACVTAFAGPISFVGIAVPHLMKNLLQTAKPLWLIPACFFGGGAFCLLCDWIARTVFSPTELSISTVTAVFGAPIVIWMMLHREKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1G9</accession>
    <name>R5V1G9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cupin domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01826</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000186">
        <property type="protein sequence ID" value="CCZ84100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01826"/>
    <dbReference type="EnsemblBacteria" id="CCZ84100">
        <property type="protein sequence ID" value="CCZ84100"/>
        <property type="gene ID" value="BN566_01826"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051920">
        <property type="term" value="F:peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003779">
        <property type="entry name" value="CMD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02627">
        <property type="entry name" value="CMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="SSF69118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CMD" evidence="1">
        <location>
            <begin position="16"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Cupin_2" evidence="2">
        <location>
            <begin position="150"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="250" mass="27587" checksum="7E2B6197F87AE6CD" modified="2013-07-24" version="1">MVKQTAGRDALGTFAPQFAALNDDVLFGEVWSREAQLPLKLRSIVTITSLISKGIVDSSLQYHLATAKKNGVSKSEMVEILTQVAFYAGWPNAWAAFRMAKEVYADDATEEHGGFFGQGEPNTAFAKYFIGQSYLKPLTNPKETVFIANVTFEPGCRNNWHIHHAKSGGGQILLCVDGEGWYQEDGKSAQSLHPGDVVVIPPEVKHWHGAKANSWFSHLAIECPGEETSNEWLEAVTDEQYAALKEDETK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1H1</accession>
    <name>R5V1H1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01831</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000187">
        <property type="protein sequence ID" value="CCZ84105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01831"/>
    <dbReference type="EnsemblBacteria" id="CCZ84105">
        <property type="protein sequence ID" value="CCZ84105"/>
        <property type="gene ID" value="BN566_01831"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="HisKA_3" evidence="2">
        <location>
            <begin position="170"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="355" mass="39857" checksum="F5501658C676BE92" modified="2013-07-24" version="1">MYPFLEKSILFWMILISVASTIDTIHPLPVISLLLALTISALAQAFPKNWLSSVCLGGYFLLCSCYPPILLTMPLLLYDAIRAKKWYLSIFAVGALLHIATFSVLQICLFCGLIPLSILLGWRTQQWETWVQTAHQQHDHASELQQKLTQRNRQLQTAQNDAVRMATLQERNRIAREIHDNVGHMLTRALLQTGALAVCVKEEPLKASVAALQETLDSAMTSIRSSVHKLHDDAIPLEKSVQDCLQPLKDTYHITLTYDISEQIPSIVKLCLLGVLKESLSNVVKHSNGDAIQIVIREHPAFYQLSITDNGTGSHLQQTGIGLETMQDRAEQVHGLIRFFPEKDSFRVFLSIPKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1H9</accession>
    <name>R5V1H9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Myosin heavy-chain) kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01841</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000187">
        <property type="protein sequence ID" value="CCZ84115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84115">
        <property type="protein sequence ID" value="CCZ84115"/>
        <property type="gene ID" value="BN566_01841"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="SSF50978"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="NACHT" evidence="2">
        <location>
            <begin position="260"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="1125"/>
            <end position="1147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="1456" mass="167084" checksum="7F913E764ECA08D4" modified="2013-07-24" version="1">MEQELTLHTLKPLLLQSDAENCSDALFLKKLFYHIVDESVIQKKLICKSTSSLKAFMQGNRRRNRQQKRTYANYHGTELLKKTFQLEDRDQLFQNETLHQLAYNIDTMMAEAKISDWRAVQISIFKMLNEEMVYGSDMLNDIKELYRQNKISRIIAVVVLIAIFQETISDVFCWLTPKNLGEMVPAIQKAAIQMLHQAAEEQGRMLAFHSVSHKIDMPGIGNLLSTALGVQNHHIDSILVESEQNNCESLRTLFSNSPYPNIMLTGTGGAGKTFLLLDLGESLLRENSSLIPIYIPLNLLNYNTIGKQESPILHHFYQILSEEKQLPYEQFLSMLQAESDVFLFFLLDGYNEISSETAKNQLLLEIKEMKRKYPAIRFLITSRNNLADCFSTGGELPFLCKRVNPLQDTQIQDFFKQLFGTERAEQEWQTLTHSNCMDILRSPMALAMYAYTLKKDVIPTAPLPHRLPETTGELICNFIEQVKRTPTLNGTSEEREQTNFAMQMLYYIAYCMTRNGSFRMDEFSMRNTVQDAIAFYKTYSPHPMPDTFAATFWEIEHKMGFLIEPQHADQDFRFYHQNFRDYFYANFLLEIIQTAIWMQQYSTPSQSAGQLLCRFFSEPIPEEILQFLGESTLERLYLPGQPRMNDPQGSLLEQALQILSELPANSAVQKSIEQLIFAVKQTRHQNLSTFRFNDLHLETVNLNGIRLYDFQCNTLHTADFSGSFLNAYTFQPLGHSAAVHTFCTIQNNVLSISGSGIWCYNNQTQQFRFLSAYNGTYVSTSVSVPQHRLLLIGDKNGNLSFWSVSFQDKLLSIQQQPERTISLNNTIQHLLYDAAQDCCFAAIKREGIWRIPLLHPENRKFVPYPEHCRNFRKYRLAAVEDVLIFSCGKTLYQIPMHAETFCSEQIQPFLTVSTQAPEPDDAFIYDFKVLSYLHQPCFLINLRGKSRSVVLLATPNGNLEIQRKEHTDTFNGFNDLILSPDASKFCICNKVYGNREPCISEVLLSIFEKEIICKPYYGKQNFEIETACYLDSNSIMLSSLDRSLQIINTRTETICCQFLGYDAGIHSLFPVNENTLYTSSYDGTLHQLSRESWFTGSWHCVRAIPAHNNWVHQVKQFSIDGVPVLASCSHDHTIRIWNAKTETCICTISMPYLVIAVGFFPDGTVVGVTRNGIIGHYRIDLNHQQAETLSLFELQQMLECSFARTRKLCMRKKADGTWLLFLLVWTANHTTVVYQLTENDQQELQLNRCCSMDELVTENVILRDVDVAFLKDGHTRCALFCGSPYQQFQNNFFALQTDQNQQYLYRPEADTPISVSRHDALDNYNGVTAGLFLSYQGELYAAIATYSYQIIIFAVRAVTNAKIILKCVHDTPILDLQTAGNTLFAAGANGKVFQWNLAQLLPVSDTEKPLIFHSQPENVIFENISGFFMNHVRFADSHTDWSEDFRKKIEQYAENS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1J2</accession>
    <name>R5V1J2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Vitamin B12 dependent methionine synthase activation domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00340</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000225">
        <property type="protein sequence ID" value="CCZ84433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00340"/>
    <dbReference type="EnsemblBacteria" id="CCZ84433">
        <property type="protein sequence ID" value="CCZ84433"/>
        <property type="gene ID" value="BN566_00340"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008705">
        <property type="term" value="F:methionine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017342">
        <property type="entry name" value="S-AdoMet-dep_Met_synth_prd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004223">
        <property type="entry name" value="VitB12-dep_Met_synth_activ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037010">
        <property type="entry name" value="VitB12-dep_Met_synth_activ_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02965">
        <property type="entry name" value="Met_synt_B12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037984">
        <property type="entry name" value="Met_synth_TM0269_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56507">
        <property type="entry name" value="SSF56507"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0170">Cobalt</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AdoMet activation" evidence="2">
        <location>
            <begin position="89"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="227" mass="25278" checksum="982BB6072DDEE10E" modified="2013-07-24" version="1">MSPSICLSPLDRSETLRYLGMTEAAADDAFLSRLDACEAKLLRHATPRYTYCILPLTRTESVLYADTLLLEGNDIRQHLEGCDRAVLMAATLGTAVDVLIDRTQKRDMTNALLMDALANTAIEQVCDKAEQQIQETMPNRYFTWRFSPGYGDFPISEQPNLLAHLNAARQVGIITTETYLMNPRKSVSAIFGCAQHPLPKRRQGCSICKMRETCAFRKKGAHCGGTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1J8</accession>
    <name>R5V1J8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TIGR00266 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00345</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000227">
        <property type="protein sequence ID" value="CCZ84438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84438">
        <property type="protein sequence ID" value="CCZ84438"/>
        <property type="gene ID" value="BN566_00345"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002838">
        <property type="entry name" value="AIM24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036983">
        <property type="entry name" value="AIM24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016031">
        <property type="entry name" value="Trp_RNA-bd_attenuator-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01987">
        <property type="entry name" value="AIM24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51219">
        <property type="entry name" value="SSF51219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00266">
        <property type="entry name" value="TIGR00266"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="228" mass="24640" checksum="942A320A137C22A2" modified="2013-07-24" version="1">MQYEIKGGSMPVVVCTLQNGESIKTEKGAMSWMSPNMEMQTNAGGGIGKAFSRIFTGESMFQNIYTARGGTGLIAFASSFPGEILAIPISASRSIVAQKHAFLAMESGVDMKLFFQKRFGSGLFGGEGFIMQQFLGNGIVFLEIDGSVVEYELQAGQSMLLDNGYLAAMDATVQLEIEKVSRIGNVLFGGEGLFNTKVTGPGHIWLQSMPINQMASVLRPYFVSENNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1Q9</accession>
    <name>R5V1Q9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Shikimate dehydrogenase (NADP(+))</fullName>
            <shortName evidence="1">SDH</shortName>
            <ecNumber evidence="1">1.1.1.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">aroE</name>
        <name evidence="3" type="ORF">BN566_00382</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>NADP(+) + shikimate = 3-dehydroshikimate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:17737"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16630"/>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.25"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the shikimate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.25" evidence="1"/>
    <dbReference type="EMBL" id="CBAR010000240">
        <property type="protein sequence ID" value="CCZ84483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00382"/>
    <dbReference type="EnsemblBacteria" id="CCZ84483">
        <property type="protein sequence ID" value="CCZ84483"/>
        <property type="gene ID" value="BN566_00382"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00087"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004764">
        <property type="term" value="F:shikimate 3-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00222">
        <property type="entry name" value="Shikimate_DH_AroE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminiacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013708">
        <property type="entry name" value="Shikimate_DH-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022893">
        <property type="entry name" value="Shikimate_DH_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21089">
        <property type="entry name" value="PTHR21089"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08501">
        <property type="entry name" value="Shikimate_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="SSF53223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="1" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Shikimate_dh_N" evidence="2">
        <location>
            <begin position="6"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Shikimate binding" evidence="1">
        <location>
            <begin position="14"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="277" mass="30784" checksum="35F412FB2544255C" modified="2013-07-24" version="1">MEHYTLIGHPLGHSMSPWIHERLFALAGRKADYTLTDLVAEDLTLQIDDLRAMQGFNITIPHKMEIIPFLDDMAESAQRYQSVNCVVNRRGNLIGYNTDCDGFLRSVESFPLDGKVLLLGCGGVGRMMATEAVLHGADLTIGILERHIPMAKTFLEELRELVPEASVRYALTAELDEPFDLVLNSTPVGMYPKVDAMPLTESLLQQCGAVFDAIYNPTETKLIQTAKAMGKPVCGGAAMLVWQAVKAHEIWNEDTYSNEQIQALIAEMEAEIQRLFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1U2</accession>
    <name>R5V1U2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00397</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000240">
        <property type="protein sequence ID" value="CCZ84498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84498">
        <property type="protein sequence ID" value="CCZ84498"/>
        <property type="gene ID" value="BN566_00397"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="70" mass="8162" checksum="ED87F1F5B55BFCD7" modified="2013-07-24" version="1">MEKKEGKQMFQVLAKPISYALIVDARQADAFLKHKPNPAMKERILRDAKRLEEQMKPHEAEQPVRESDCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1X4</accession>
    <name>R5V1X4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator AbrB family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00218</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000210">
        <property type="protein sequence ID" value="CCZ84305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00218"/>
    <dbReference type="EnsemblBacteria" id="CCZ84305">
        <property type="protein sequence ID" value="CCZ84305"/>
        <property type="gene ID" value="BN566_00218"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007159">
        <property type="entry name" value="SpoVT-AbrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037914">
        <property type="entry name" value="SpoVT-AbrB_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04014">
        <property type="entry name" value="MazE_antitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00966">
        <property type="entry name" value="SpoVT_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89447">
        <property type="entry name" value="SSF89447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01439">
        <property type="entry name" value="lp_hng_hel_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51740">
        <property type="entry name" value="SPOVT_ABRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SpoVT-AbrB" evidence="2">
        <location>
            <begin position="5"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="179" mass="19538" checksum="701DA17A2C5C962B" modified="2013-07-24" version="1">MKATGIVRRIDDLGRVVIPKEIRRTMRIREGDPLEIYTSNDGEVIFKKYSPIGEMSEHAAQVADIMHRLAGCPVAIFDRDHVVSVSGAAKKEWNARRVSPELEDLMEQRKMYYAENGASGMIPAEGVEKHAVACLPIISAGDVTGAVAFLDDGSGATVNHCQKTLIQAASQFLGKQLES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1X7</accession>
    <name>R5V1X7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyltransferase group 2 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00223</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000212">
        <property type="protein sequence ID" value="CCZ84310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00223"/>
    <dbReference type="EnsemblBacteria" id="CCZ84310">
        <property type="protein sequence ID" value="CCZ84310"/>
        <property type="gene ID" value="BN566_00223"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="234"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="269"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_trans_2-like" evidence="2">
        <location>
            <begin position="7"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="326" mass="36380" checksum="A542EDD9B308C242" modified="2013-07-24" version="1">MKLMIQIPCYNEAETLGLAIHDLPTHIDGIDTIEYLIINDGSKDATVEAARKLGVHHIVSFRRNKGLARGFLAGLDACLRLGADIIVNTDADNQYCGADIEKLVQPILQKKADMVIGERPIDETEHFSWKKKKFQHLGSWVVRVASQTDVPDAPSGFRAYSREAAMRMNVVNEYTYTLETIIQAGQSKMAIGSVPIRTNPETRPSRLFSSMWRYMKRSASVITRSFVMYKPLKFFGTLGTIIFALGVLLGIRFLVYLGMGEGSGHVQSLILTAVLLMIGFQTIIMGLLGDTVAANRKILEDVQYHVRKMDYDDHPATELEDDKPTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>R5V271</accession>
    <name>R5V271_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Probable dual-specificity RNA methyltransferase RlmN</fullName>
            <ecNumber evidence="11">2.1.1.192</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">23S rRNA (adenine(2503)-C(2))-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">23S rRNA m2A2503 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Ribosomal RNA large subunit methyltransferase N</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">tRNA (adenine(37)-C(2))-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">tRNA m2A37 methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">rlmN</name>
        <name evidence="13" type="ORF">BN566_00486</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>adenosine(2503) in 23S rRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = 2-methyladenosine(2503) in 23S rRNA + 5'-deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42916"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10152"/>
            <dbReference type="Rhea" id="RHEA-COMP:10282"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74497"/>
            <dbReference type="EC" id="2.1.1.192"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:43332"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10485"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74497"/>
            <dbReference type="EC" id="2.1.1.192"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="11">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="11">Reaction proceeds by a ping-pong mechanism involving intermediate methylation of a conserved cysteine residue.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the radical SAM superfamily. RlmN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.192" evidence="11"/>
    <dbReference type="EMBL" id="CBAR010000257">
        <property type="protein sequence ID" value="CCZ84633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00486"/>
    <dbReference type="EnsemblBacteria" id="CCZ84633">
        <property type="protein sequence ID" value="CCZ84633"/>
        <property type="gene ID" value="BN566_00486"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070040">
        <property type="term" value="F:rRNA (adenine-C2-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002935">
        <property type="term" value="F:tRNA (adenine-C2-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01849">
        <property type="entry name" value="RNA_methyltr_RlmN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040072">
        <property type="entry name" value="Methyltransferase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027492">
        <property type="entry name" value="RNA_MTrfase_RlmN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004383">
        <property type="entry name" value="rRNA_lsu_MTrfase_RlmN/Cfr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30544">
        <property type="entry name" value="PTHR30544"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006004">
        <property type="entry name" value="CHP00048"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00275">
        <property type="entry name" value="adenosine_C2_methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00048">
        <property type="entry name" value="rRNA_mod_RlmN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9 11" id="KW-0408">Iron</keyword>
    <keyword evidence="10 11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 11 13" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="7 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 11 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Radical SAM core" evidence="12">
        <location>
            <begin position="99"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="11">
        <location>
            <begin position="160"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="11">
        <location>
            <begin position="215"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="93"/>
        </location>
    </feature>
    <feature type="active site" description="S-methylcysteine intermediate" evidence="11">
        <location>
            <position position="333"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CCZ84633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="344" mass="38750" checksum="687F9D9B65DF53EF" modified="2013-07-24" version="1">MEQQDILSMTLPELQAAVQELSEPKFRAKQIFNWLHQKRVTDFSEMTTLSLPFRERLASDFCINRLKIAKRLASSVDDTVKYLYELPDGNYVETVCMQYHHGTSLCISTQVGCRMGCRFCASAIAGFVRNLTPAEMLLQIYETERAQNCKISSLVLMGIGEPLDNFDNVLRFLQLLSDPNGYGMSLRHVTVSTCGVVPKILELAEQRLGLTLSVSLHCPNDAGRSAIMPINRSYPIDVLLDACQTYFEKTGRRVTFEYAVMDQVNSAEQDAAALAERLKGLHAHVNLIPINPVKERNFHTLKKTVLQFQKTLERYGIPATIRRTLGSDIEAACGQLRRDAIQQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5V2B4</accession>
    <name>R5V2B4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00541</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000267">
        <property type="protein sequence ID" value="CCZ84688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84688">
        <property type="protein sequence ID" value="CCZ84688"/>
        <property type="gene ID" value="BN566_00541"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="199" mass="22089" checksum="2B03BE01BF521804" modified="2013-07-24" version="1">MRMRVKKHVCVQKHIPSVKKHPLAWLWVALCVLILLESRVCDWTERLVAEKSSFEGSGSLQIEQAILFAGNAGGSDSLYDLTVTLHNVGDTLEKYLPQIYVTESEQPDNSKVHLSLQPISLYEEINAAGVNNYDIDLCIPPGQTVTVSYSVTASDYQEIQALRDDGKTVSVSLSNRKAKQNVCVPLQWMEIETGEKGGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5V2B7</accession>
    <name>R5V2B7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal RNA small subunit methyltransferase E</fullName>
            <ecNumber evidence="6">2.1.1.193</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_00354</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">Specifically methylates the N3 position of the uracil ring of uridine 1498 (m3U1498) in 16S rRNA. Acts on the fully assembled 30S ribosomal subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>S-adenosyl-L-methionine + uridine(1498) in 16S rRNA = H(+) + N(3)-methyluridine(1498) in 16S rRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42920"/>
            <dbReference type="Rhea" id="RHEA-COMP:10283"/>
            <dbReference type="Rhea" id="RHEA-COMP:10284"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74502"/>
            <dbReference type="EC" id="2.1.1.193"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the RNA methyltransferase RsmE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.193" evidence="6"/>
    <dbReference type="EMBL" id="CBAR010000233">
        <property type="protein sequence ID" value="CCZ84455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00354"/>
    <dbReference type="EnsemblBacteria" id="CCZ84455">
        <property type="protein sequence ID" value="CCZ84455"/>
        <property type="gene ID" value="BN566_00354"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006700">
        <property type="entry name" value="rRNA_ssu_MeTrfase-E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30027">
        <property type="entry name" value="PTHR30027"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04452">
        <property type="entry name" value="Methyltrans_RNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015601">
        <property type="entry name" value="MTase_slr0722"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00046">
        <property type="entry name" value="TIGR00046"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 7" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR015601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ84455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="239" mass="25958" checksum="82983E42D15C3C5D" modified="2013-07-24" version="1">MPRFFLETIAEDDIQITGADAHHIGYALRMRPGEHLTVCANNIDYDCVIREITKDTVFLDAVSKHACAAEPSVFVTLYQAVPKAGKLEEIIQKSIELGVSRIVPVLTARCVARPKAAEFQKKLPRLQKIAESAAKQSGRGCIPEVAPLHTFAEALESAKQADCGVFLYEGGGIRFSEVPLTGKKQIALWIGSEGGFDPKEAEAAKAAGFYPVWLGERILRCETAPQAALAILMHLTGNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>R5V2Z3</accession>
    <name>R5V2Z3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">S-adenosylmethionine:tRNA ribosyltransferase-isomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_00498</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000259">
        <property type="protein sequence ID" value="CCZ84645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00498"/>
    <dbReference type="EnsemblBacteria" id="CCZ84645">
        <property type="protein sequence ID" value="CCZ84645"/>
        <property type="gene ID" value="BN566_00498"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008616">
        <property type="term" value="P:queuosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1780.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003699">
        <property type="entry name" value="QueA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042118">
        <property type="entry name" value="QueA_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036100">
        <property type="entry name" value="QueA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30307">
        <property type="entry name" value="PTHR30307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02547">
        <property type="entry name" value="Queuosine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111337">
        <property type="entry name" value="SSF111337"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-0671">Queuosine biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ84645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="155" mass="17406" checksum="6428F7F8838612C4" modified="2013-07-24" version="1">MLQALREKGVETAFVTLHVGLGTFRPVKEDDVLQHKMHSEHYHLPQETVDKILQTKQNGGRVIAVGTTSCRTLEAVATEHDGKLVAADGYTDIFIYPGYQFKVLDGLVTNFHLPESTLIMLVSALLGYEKTMHAYDVAVKERYRFFSFGDAMCIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5V2Z6</accession>
    <name>R5V2Z6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00503</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000265">
        <property type="protein sequence ID" value="CCZ84650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84650">
        <property type="protein sequence ID" value="CCZ84650"/>
        <property type="gene ID" value="BN566_00503"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004394975" evidence="3">
        <location>
            <begin position="29"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="303"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="266"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="331" mass="34263" checksum="410BBCA875E611C9" modified="2013-07-24" version="1">MKRTILAVSAFAASLMVSAVAVSAPVQAEEIFHSRKAVAEVVATTPLYEAQDDTQTTTTTTDLSGGNNVETTTTLNSAVTGSEETTTTTTTVPLQVVTVNATLHASFNGDKEDAPSVPLSISSDSLQESYTISCDLTDPSKALDYVYLDFALPDAKQSIRTFCPNFTPAVTKVSTGADLLTDVPYTMGANAVNYTYTDATGNQYVRVVLYDATATGDGYLAQNAAITDKLQLTFQLTGLESFDPTVTTTATSDTVTTTTVNVNGAYDTSGKGGSYNNGSYNSGKGGSSSAPTSTSTPTQTADLSVGAIVVGAVAAVSLAGAAFTVTRRKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>R5V3V1</accession>
    <name>R5V3V1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">23S rRNA m(5)U-1939 methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_00584</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000007">
        <property type="protein sequence ID" value="CCZ82779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00584"/>
    <dbReference type="EnsemblBacteria" id="CCZ82779">
        <property type="protein sequence ID" value="CCZ82779"/>
        <property type="gene ID" value="BN566_00584"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010280">
        <property type="entry name" value="U5_MeTrfase_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11061">
        <property type="entry name" value="PTHR11061"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01938">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05958">
        <property type="entry name" value="tRNA_U5-meth_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51687">
        <property type="entry name" value="SAM_MT_RNA_M5U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TRAM" evidence="5">
        <location>
            <begin position="10"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="416"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="320"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="341"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="389"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ82779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="459" mass="50734" checksum="E060EF15FBB11A36" modified="2013-07-24" version="1">MKSNANRESVLQKNQSYATEITDLTVEGNGVCHIQGMTVFVPHTAPGDQVRVKIVKVCKQYAFGIIEEMETPASCRIESDCPYPACGGCTFRHISYAAELQWKEKLVQDAFHRIGKLHPEFLPIVGGKQRSHYRNKAQYPVAVGADGKPVCGFYAKRSHRVIPVRDCQLQPVLFQQVLDVILQYVAEKSISVYQEETGTGILRHIYLRQGWHTGELMVCLVVRKPIERQLKGLIERLTTAFPQVKSIVMNCNPARTNVILGEKNVLLWGSETITDILCGNRISLSPHAFYQVNTEQAERLYGIAKEFAALQGTERLLDLYCGAGTIGLSMADAVVSVLGVEIVPQAVENAKENARQSGISNAEFLCGDAGTVAQKLEQEQLQPDVIVVDPPRKGCDQNALDAMAKMQPERIVMISCHPATAARDCAVLQEMGYVVQKVQPVDLFPGTGHVECVVLMSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>R5V454</accession>
    <name>R5V454_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Adenylate/guanylate cyclase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN566_00701</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the P(II) protein family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ammonia transporter channel (TC 1.A.11.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000022">
        <property type="protein sequence ID" value="CCZ82899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00701"/>
    <dbReference type="EnsemblBacteria" id="CCZ82899">
        <property type="protein sequence ID" value="CCZ82899"/>
        <property type="gene ID" value="BN566_00701"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006808">
        <property type="term" value="P:regulation of nitrogen utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001905">
        <property type="entry name" value="Ammonium_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002187">
        <property type="entry name" value="N-reg_PII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017918">
        <property type="entry name" value="N-reg_PII_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00543">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00340">
        <property type="entry name" value="PIIGLNB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00938">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="SSF54913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00836">
        <property type="entry name" value="amt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00638">
        <property type="entry name" value="PII_GLNB_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51343">
        <property type="entry name" value="PII_GLNB_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="57"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="167"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="205"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="237"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="267"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="290"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="323"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="363"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium_transp" evidence="8">
        <location>
            <begin position="17"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ82899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="572" mass="60673" checksum="7A1790AF293461FF" modified="2013-07-24" version="1">MDETKLLDLVGGQVFGVWFLVGAALVFFMQCGFAMVETGFTRAKNAGNIIMKNLMDFCIGTVVFILIGFSLLLGEDWLGLIGKPGFDIFSDYANFNWSGFVFNLVFCATAATIVSGAMAERTKFISYCIYSAVISALIYPVEAHWVWGGGWLSTLDTPFHDFAGSGVIHFVGGLCALIGASILGPRIGKFTKDENGNVKVNAIPGHSLTIGALGCFILWFGWYGFNGAAATSVEQLGSIFVTTTIAPAIATFVCMVFTWLKYGKPDVSMCLNASLAGLVGITAGCDVMDATGAIIVGIVSGLLVCFGVWLLDHVLHVDDPVGAVAVHFFNGLWGIIAVGLFATPDAPLSGCTGLFYGGGFSLLGSQLLGLLCIAAWTAVLITITFLIIKHTIGLRASAEEEVIGLDATEHNLPSAYADFMPSAATVSMDTENASVAGVDLPEPQLIVKPATGTAKMSKIVVICKQEKLAVLQSALNKIGVNGMTVTQVQGYGTQKGNSQYYRGVRVDGPKLLPKVKIEIVVTSIPVETIVDVCKKVLYTGQIGDGKIFVYDVEEVVKVRTGETGYDALQGDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5V4E2</accession>
    <name>R5V4E2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BFD domain protein (2Fe-2S)-binding domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00774</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000034">
        <property type="protein sequence ID" value="CCZ82974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00774"/>
    <dbReference type="EnsemblBacteria" id="CCZ82974">
        <property type="protein sequence ID" value="CCZ82974"/>
        <property type="gene ID" value="BN566_00774"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007419">
        <property type="entry name" value="BFD-like_2Fe2S-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041854">
        <property type="entry name" value="BFD-like_2Fe2S-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04324">
        <property type="entry name" value="Fer2_BFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fer2_BFD" evidence="1">
        <location>
            <begin position="23"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="109" mass="12303" checksum="61036EE5AC85499C" modified="2013-07-24" version="1">MENILDRSVQLGEFVPEPDDDRIVCRCEEVTKGEIRRAVHDGMFTITEIRRYLRAGMGLCQGQTCAKLVKRIVAQELQVSPAVLEPATSRAPMRPLEMKIFAAEAKEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5V4K7</accession>
    <name>R5V4K7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dockerin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00802</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000038">
        <property type="protein sequence ID" value="CCZ83009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83009">
        <property type="protein sequence ID" value="CCZ83009"/>
        <property type="gene ID" value="BN566_00802"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002105">
        <property type="entry name" value="Dockerin_1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00404">
        <property type="entry name" value="Dockerin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004406717" description="Dockerin domain-containing protein" evidence="2">
        <location>
            <begin position="26"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="3">
        <location>
            <begin position="28"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="204" mass="22059" checksum="049827C0B32D581D" modified="2013-07-24" version="1">MKKTLYACMAAVLALASLPVLGASAEETTYALGDVDMDGVVTGHDTAMVSRYVLDDTYTLTEEQLKLADVNEDGVVDQADADKLYTEMQVYSLGDIDMSGMAEVGDTSDILVYYAKVSAGQNVELTAVQKNLADVDLNGTVDITDACYNLAGYARAAAGQKAYNRDNEKQYYYAPVKIVIQDPDDPNYTGDLEPDTVYVPKTKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5V4R1</accession>
    <name>R5V4R1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00872</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000051">
        <property type="protein sequence ID" value="CCZ83084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00872"/>
    <dbReference type="EnsemblBacteria" id="CCZ83084">
        <property type="protein sequence ID" value="CCZ83084"/>
        <property type="gene ID" value="BN566_00872"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004792">
        <property type="entry name" value="BaiN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023166">
        <property type="entry name" value="HI0933-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42887">
        <property type="entry name" value="PTHR42887"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00275">
        <property type="entry name" value="TIGR00275"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="412" mass="44232" checksum="EF1287487BA9ABE4" modified="2013-07-24" version="1">MEADVFIIGGGAAGTVAALLLAKAGKHCIILEKNGQIGRKLRITGKGRCNLTNACDLDTLMANVPRNPKFLYSAFARWMPEDVMAYFEQLGVPLKVERGNRVFPVSDRAEDIVAALQRALKRAGIPVYRETVQQLLLQDGRCTGVQTASGKQYAANCVLLAAGGASYPATGSTGDGYTLAKQAGHTIVPPLPALVPLEVQEKWCSDLMGLSLRNVSLKLYNGKKCCYDALGEMLFTHFGVSGPLVLTASSLLPEQIPKDGSCRLVLNLKPGLTPEQLDKRIQRDCAAHPNQAMGTIFRGLLPLKLVPVFLEAVHIPPETKANALTKEQRRILVQGLQAFPLHITARRSLKEAIITRGGVSVREVQAKTMASKCCEGLYFAGEVLDVDAYTGGFNLQIAFSTAACAADAIGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5V5C8</accession>
    <name>R5V5C8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyl hydrolase family 32</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000089">
        <property type="protein sequence ID" value="CCZ83339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01120"/>
    <dbReference type="EnsemblBacteria" id="CCZ83339">
        <property type="protein sequence ID" value="CCZ83339"/>
        <property type="gene ID" value="BN566_01120"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013189">
        <property type="entry name" value="Glyco_hydro_32_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08244">
        <property type="entry name" value="Glyco_hydro_32C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="SSF49899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyco_hydro_32C" evidence="1">
        <location>
            <begin position="51"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="156" mass="17627" checksum="25670A9EC3EEF4B6" modified="2013-07-24" version="1">MIAWARMPQAVDGKWNGMMCIPRVVSVKNQQIYFQPHPHIRNAFMKEISSPEQAGKSGYLVKTTLENGDYINIGGYQIWRKQDKLFTDRSTVFVKGGNYRLIAETPVLRDGCELEIYVDANLIEVYANQGEAVISSVVYGLSDIIDGKAYTLYIIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>R5V5X8</accession>
    <name>R5V5X8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidoglycan glycosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.129</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN566_01268</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n)-di-trans,octa-cis-undecaprenyl diphosphate + beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate = [GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-di-trans-octa-cis-undecaprenyl diphosphate + di-trans,octa-cis-undecaprenyl diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23708"/>
            <dbReference type="Rhea" id="RHEA-COMP:9602"/>
            <dbReference type="Rhea" id="RHEA-COMP:9603"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58405"/>
            <dbReference type="ChEBI" id="CHEBI:60033"/>
            <dbReference type="ChEBI" id="CHEBI:78435"/>
            <dbReference type="EC" id="2.4.1.129"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the transpeptidase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the glycosyltransferase 51 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.129" evidence="4"/>
    <dbReference type="EMBL" id="CBAR010000118">
        <property type="protein sequence ID" value="CCZ83499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01268"/>
    <dbReference type="EnsemblBacteria" id="CCZ83499">
        <property type="protein sequence ID" value="CCZ83499"/>
        <property type="gene ID" value="BN566_01268"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3810.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001264">
        <property type="entry name" value="Glyco_trans_51"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036950">
        <property type="entry name" value="PBP_transglycosylase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00912">
        <property type="entry name" value="Transgly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0046">Antibiotic resistance</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="18"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="Transgly" evidence="8">
        <location>
            <begin position="61"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ83499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="247" mass="27962" checksum="F887BFE748694A66" modified="2013-07-24" version="1">MQAETMQKQRTTSKTKSYICRILLLLGVLLLAMGLWYGVQGYQMYRNVLAETPIAEKTAAIEQQESFVPYDALPQIYVDAVISVEDQRFFSHSGVDISAMIRALWNDLRTRSFAEGGSTISQQLAKNQYFTQEKTLQRKLAEMFLAHAWEQVCEKQELFALYVNTIYFGDGYTGISEAAEGYFHTTPQQLTDYEAVMLAGLPNAPSAYALQEHADLAEQRMQQVLQAMVANQKLTQSEANEILQEKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5V630</accession>
    <name>R5V630_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Dockerin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01327</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000130">
        <property type="protein sequence ID" value="CCZ83559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83559">
        <property type="protein sequence ID" value="CCZ83559"/>
        <property type="gene ID" value="BN566_01327"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002105">
        <property type="entry name" value="Dockerin_1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00404">
        <property type="entry name" value="Dockerin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004394700" description="Dockerin domain-containing protein" evidence="1">
        <location>
            <begin position="27"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="2">
        <location>
            <begin position="213"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="285" mass="30399" checksum="BD833B783692C34B" modified="2013-07-24" version="1">MKGFQRVVGCLLAVGFLGMMPTPAIAAETSAVIEMPYASSLTAKDGSIRVVLQDSRKVHVTIQKMTQEGSDAYYDVVLDPTQSSAATTEYVFYLDCCEYNLSTASYVSTYQLTIADESTPEAVYTETDIAIFDPHFSDTVNTTCYRYAVTMLEGTTGGVVATVPKENISNGVRTVENQVTLRYVPQTTAITTTSTTTTTTPFTTTSATTTTAPAFKLGDVDGNGMIEVTDAVLVLTYYAKSAAGIAVTIDSRSADVNQDGIVDVVDAVGILTYYARQAAGLPPTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5V675</accession>
    <name>R5V675_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00146</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Transcriptional repressor of xylose-utilizing enzymes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ROK (NagC/XylR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000146">
        <property type="protein sequence ID" value="CCZ83619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00146"/>
    <dbReference type="EnsemblBacteria" id="CCZ83619">
        <property type="protein sequence ID" value="CCZ83619"/>
        <property type="gene ID" value="BN566_00146"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042732">
        <property type="term" value="P:D-xylose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000600">
        <property type="entry name" value="ROK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18964">
        <property type="entry name" value="PTHR18964"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0859">Xylose metabolism</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="403" mass="45921" checksum="530CBAD80A1C8901" modified="2013-07-24" version="1">MWKPVIARQMERKWMYLQVLQLIQQYGAISRVEIANKLHMTRASVTLLIHEMMEKGVLEDIGTCPTSEGKGRRKLLMDVHPSRWLLIGISIQGNHLVVGLTTLGMNTLEKQCIPFPVMQASWASIQEQMIITVRQILKSNYIEESQILGLGVGWMPSVLQHFFPDATRQEQQMTELQQILRDALHVPVFWGNALPSMAVYCLYQKPKQEIIALFCADGADYYVSIVCRSHTMECLNGKPISMQGLPLSPNQTVGDLLTPVALQQRVKPYYSAEQTPVLYDLTGGKLETMTLPDLFTAACAEGKDFDPILSDLMEEMMQQFCQFWSSILVMYPIEQLYLYRPDFTLPHWQEIYRYAKQYMKPELAAKLSCGDLTEKCQYAGGVFYALDGGIFKLCTAEEQKTSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5V6A9</accession>
    <name>R5V6A9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Erf family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01395</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000148">
        <property type="protein sequence ID" value="CCZ83659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83659">
        <property type="protein sequence ID" value="CCZ83659"/>
        <property type="gene ID" value="BN566_01395"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007499">
        <property type="entry name" value="ERF_bacteria_virus"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04404">
        <property type="entry name" value="ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="188" mass="20466" checksum="4FF8ADF53B600E8A" modified="2013-07-24" version="1">MLAIQNELKAPKGQYNSFGKYKYRSAEDILEAVKPLAEQHSVLIYCSDDIVMVGNRIYVKATATAEDVTGRCSAITVTAFAREPDDKKGMDASQITGTASSYARKYALNGLLCIDDAKDADTDAYQTAGNVPAATQQQPRQQIYCSSCKKPIQAAKGKDGRLYQPIDIYKQCRGLCINCFQASKGAGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5V6D0</accession>
    <name>R5V6D0_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 2">Ribosome biogenesis GTPase A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_01420</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Required for a late step of 50S ribosomal subunit assembly. Has GTPase activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class YlqF/YawG GTPase family. MTG1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000153">
        <property type="protein sequence ID" value="CCZ83684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01420"/>
    <dbReference type="EnsemblBacteria" id="CCZ83684">
        <property type="protein sequence ID" value="CCZ83684"/>
        <property type="gene ID" value="BN566_01420"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030378">
        <property type="entry name" value="G_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023179">
        <property type="entry name" value="GTP-bd_ortho_bundle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019991">
        <property type="entry name" value="GTP-bd_ribosome_bgen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016478">
        <property type="entry name" value="GTPase_MTG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006230">
        <property type="entry name" value="MG442"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03596">
        <property type="entry name" value="GTPase_YlqF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51721">
        <property type="entry name" value="G_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CP-type G" evidence="4">
        <location>
            <begin position="19"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="64"/>
            <end position="67"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="137"/>
            <end position="142"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00014898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR006230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR006230-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="290" mass="32624" checksum="8212326B1962B1F0" modified="2013-07-24" version="1">MSEIQMGTIQWFPGHMTKTRRMIAANLSLVDAVVEILDARIPSSSQNPEMQRLVKEKPRMLLLNKADMADPNATARWVQYYQKQNLLALPLDCKTGKGIKQFVPMVRNQLLKPLMEKRAKAGIVGAPIRLMIVGIPNVGKSSFINRMAQSKKAKVEDRPGVTRTKQWVKIGDQMELLDMPGVLWPKFDDQEVAKRLAFTGAIKDDIMDLESLAMLLLEFLAERYPKALEARYKMQVSDADSGLQLLEQLGRKRGMLISGGEVNYERAAITVLDEFRSGTLGRLTLELPEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>R5V6D4</accession>
    <name>R5V6D4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 11">Trigger factor</fullName>
            <shortName evidence="9">TF</shortName>
            <ecNumber evidence="9">5.2.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">PPIase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">tig</name>
        <name evidence="13" type="ORF">BN566_01425</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation. Functions as a peptidyl-prolyl cis-trans isomerase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9 10">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="9">About half TF is bound to the ribosome near the polypeptide exit tunnel while the other half is free in the cytoplasm.</text>
    </comment>
    <comment type="domain">
        <text evidence="9">Consists of 3 domains; the N-terminus binds the ribosome, the middle domain has PPIase activity, while the C-terminus has intrinsic chaperone activity on its own.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9 11">Belongs to the FKBP-type PPIase family. Tig subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="9"/>
    <dbReference type="EMBL" id="CBAR010000153">
        <property type="protein sequence ID" value="CCZ83689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01425"/>
    <dbReference type="EnsemblBacteria" id="CCZ83689">
        <property type="protein sequence ID" value="CCZ83689"/>
        <property type="gene ID" value="BN566_01425"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00303">
        <property type="entry name" value="Trigger_factor_Tig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005215">
        <property type="entry name" value="Trig_fac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008880">
        <property type="entry name" value="Trigger_fac_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037041">
        <property type="entry name" value="Trigger_fac_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008881">
        <property type="entry name" value="Trigger_fac_ribosome-bd_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036611">
        <property type="entry name" value="Trigger_fac_ribosome-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027304">
        <property type="entry name" value="Trigger_fact/SurA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30560">
        <property type="entry name" value="PTHR30560"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05698">
        <property type="entry name" value="Trigger_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05697">
        <property type="entry name" value="Trigger_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003095">
        <property type="entry name" value="Trigger_factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102735">
        <property type="entry name" value="SSF102735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109998">
        <property type="entry name" value="SSF109998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00115">
        <property type="entry name" value="tig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9 11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 9 11" id="KW-0132">Cell division</keyword>
    <keyword evidence="5 9 11" id="KW-0143">Chaperone</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9 10" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="12">
        <location>
            <begin position="164"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CCZ83689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="442" mass="49439" checksum="C5781F17EDA1AF0C" modified="2013-07-24" version="1">MILKSSEKTDVNMTELVATVDAETFEKAIEAVYQQQKKNISLPGFRKGKVPRRLCERTYGEGVFFEDALNLILNMEMPGVIAEAALNLVDAPKVEVTSVSKEDGATVKIICVTKPEIHIADYKGMTAPKEDKEITDEDVAKQAENVCKRNAKMVSVDDRAAEMGDEVTLDFEGFFGDTPFEGGKGEDFQLKLGSGQFIPGFEEQVAGHKIDEDFDVTVTFPEDYQMTDYAGKEAVFKCKIHSISKEEIPELTDDFVKEISDFDTVDEWKADAKKKLEENAEQTAKVGFENALVAKLIDKVQDPIPHCMFEQRADGMMDAFANQLKGQNVDLDLYLQYTGMTKDQLKETYLERAENEVKLRLALEKIAELEGLEVADEELDAELTKLAEQYNMTLDDVKARIPVAEYKLDMLVTKALDLVKDSAVVDNSEPAEAEKAEDKEAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5V6D9</accession>
    <name>R5V6D9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">N-acetylmuramoyl-L-alanine amidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01430</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000153">
        <property type="protein sequence ID" value="CCZ83694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01430"/>
    <dbReference type="EnsemblBacteria" id="CCZ83694">
        <property type="protein sequence ID" value="CCZ83694"/>
        <property type="gene ID" value="BN566_01430"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008745">
        <property type="term" value="F:N-acetylmuramoyl-L-alanine amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009253">
        <property type="term" value="P:peptidoglycan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02696">
        <property type="entry name" value="MurNAc-LAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002508">
        <property type="entry name" value="MurNAc-LAA_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003646">
        <property type="entry name" value="SH3-like_bac-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01520">
        <property type="entry name" value="Amidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08239">
        <property type="entry name" value="SH3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00646">
        <property type="entry name" value="Ami_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51781">
        <property type="entry name" value="SH3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SH3b" evidence="1">
        <location>
            <begin position="185"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="246" mass="26866" checksum="5C96387209DF40B0" modified="2013-07-24" version="1">MPNVFLSPSTQEWNPYVNGGSEEFYMNQIADRMEPYLRSSGISYTRNDPNRNAAGAIADSNAGSYDVHLALHSNAAPESLSGKLRGIDIYYAPGSRFSQILANILADRLKTIYPIPDQCRARPTTTLGEVIKTKAVAVLCELGYHDNEQDANWIRNNLNRIAITLVQGLCEYFGIPFISAGPVLSGTVQTDGSGLNLRAFPSTKGRILASIPNGTKIPLYGKTGNWYVTSYRGLTGYSNADYLAIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5V6T3</accession>
    <name>R5V6T3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">tRNA-dihydrouridine synthase</fullName>
            <ecNumber evidence="10">1.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">BN566_01559</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 10">Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a 5,6-dihydrouridine in tRNA + NAD(+) = a uridine in tRNA + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:54452"/>
            <dbReference type="Rhea" id="RHEA-COMP:13339"/>
            <dbReference type="Rhea" id="RHEA-COMP:13887"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 5,6-dihydrouridine in tRNA + NADP(+) = a uridine in tRNA + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:23624"/>
            <dbReference type="Rhea" id="RHEA-COMP:13339"/>
            <dbReference type="Rhea" id="RHEA-COMP:13887"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3 10">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the dus family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.-" evidence="10"/>
    <dbReference type="EMBL" id="CBAR010000165">
        <property type="protein sequence ID" value="CCZ83824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01559"/>
    <dbReference type="EnsemblBacteria" id="CCZ83824">
        <property type="protein sequence ID" value="CCZ83824"/>
        <property type="gene ID" value="BN566_01559"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017150">
        <property type="term" value="F:tRNA dihydrouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02801">
        <property type="entry name" value="DUS_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001269">
        <property type="entry name" value="DUS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024036">
        <property type="entry name" value="tRNA-dHydroUridine_Synthase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004652">
        <property type="entry name" value="tRNA_dU_NifR3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018517">
        <property type="entry name" value="tRNA_hU_synthase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006621">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00737">
        <property type="entry name" value="nifR3_yhdG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01136">
        <property type="entry name" value="UPF0034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6 10" id="KW-0288">FMN</keyword>
    <keyword evidence="9 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 10" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Dus" evidence="12">
        <location>
            <begin position="18"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00002790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR006621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR006621-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CCZ83824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="328" mass="35551" checksum="28DE265B2A8F3F26" modified="2013-07-24" version="1">MQQTIKLGNLTLPRTAGLAPMAGVADRAYRILCKEHQAALLVSEMISAKGLCYGDPKTAQFCTITDAERPMGLQLFGSEPDFMAKAVQIVLPYQPDWIDINMGCPVHKVITTGSGSALMQKPDLAADLVRAAVSVSPVPITVKFRAGWDDNDRNAVSFAKKMEQAGVAAVAVHGRTKAQLYSGKADWEIIRQVKESVSIPVFGNGDVTDAASCLKMYEQTGCDLVLVGRGSYGNPFIFSEIAAAMAGTPYQKPTLSEKMDTMLYHIRLILQLADGKPPELAIREARKHAAWYISGLHGAAAFRARCYQLQSYAEAEQLAADVKEANPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7B7</accession>
    <name>R5V7B7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Citrate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN566_01713</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000178">
        <property type="protein sequence ID" value="CCZ83984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83984">
        <property type="protein sequence ID" value="CCZ83984"/>
        <property type="gene ID" value="BN566_01713"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004680">
        <property type="entry name" value="Cit_transptr-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03600">
        <property type="entry name" value="CitMHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004394724" evidence="7">
        <location>
            <begin position="32"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="80"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="118"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="205"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="250"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="279"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="314"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="348"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="CitMHS" evidence="8">
        <location>
            <begin position="10"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ83984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="371" mass="41021" checksum="9B3DEF1B2AF37353" modified="2013-07-24" version="1">MKWMLFFKKHVVLILAALAAGISMFWNPPSAATWGYLDWNVLSMLFCLMAVVAGFRKIGVFQSLADVCCKHLKSSRMLAFLLWNLCFFLSMLVTNDVALITFVPFTCSLLQRDDQRKILLRIVVIEAAAANLGSMATPIGNPQNLYLYSTYSFSLGTFFKTILPIVGISYIGLLFSLLLIPQQPLASEIPTESHLSLSAQKKNLIIYSVLGVVCLATVAGFVTHVICFCVVLLGMIVTDRALIRRVDWELLLTFVCFFLFSGNLQQIEPVRNLMMQGLSGGVCLTAAIVSQVISNVPAAVLLSGFTEQGKELLLGVDIGGLGTPIASLASLIAYQYYAKSKLANPAKYLQFFFIYQFIGLFFLLAMKFMML</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7L1</accession>
    <name>R5V7L1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metallo-beta-lactamase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01810</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000186">
        <property type="protein sequence ID" value="CCZ84084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01810"/>
    <dbReference type="EnsemblBacteria" id="CCZ84084">
        <property type="protein sequence ID" value="CCZ84084"/>
        <property type="gene ID" value="BN566_01810"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="14"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="242" mass="27283" checksum="A912055EC1DBD00B" modified="2013-07-24" version="1">MLQFFGRGSAFADQHNAAFFREQGDLILLDCPGTAFQKVKHFNWSTVSSVYVLVTHTHGDHIGGLGTLLQYLFFTQHKRLTVVAPSELVREDLQLQLIRIEGCQPEWFSLLTVQQLQKTWLQRAVLTEHTDTLRLKCYGYVLTVAGKTVVYTGDTRTLEPFLPYLHPGSFLYTELAAIQSGVHLYVQDTLPRLKALSEQGVSVFLMHLDCEEEISRQIQGTAIQLAPLYSSFIHPLACGKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7M3</accession>
    <name>R5V7M3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Multimeric flavodoxin WrbA family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01825</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000186">
        <property type="protein sequence ID" value="CCZ84099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01825"/>
    <dbReference type="EnsemblBacteria" id="CCZ84099">
        <property type="protein sequence ID" value="CCZ84099"/>
        <property type="gene ID" value="BN566_01825"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN_red" evidence="1">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="177" mass="19458" checksum="B4410DDA77C3D723" modified="2013-07-24" version="1">MKIVVITGSHHKNGTSAHLAAAFIRGAEETGHEVCRFDAAFRQVHPCIACETCHNTDRGCAFADDMKTLYPELLEADAVAFATPIYYYAMNAQIRAVIDRFYACDAQLHHNKKTALLVTMADNTMESAAGAIASFQGMAKFLDWDIAGMVIGADCMDVPALERTDYEEQAYQLGKQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7N4</accession>
    <name>R5V7N4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Hydroxylamine reductase</fullName>
            <ecNumber evidence="7">1.7.99.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Hybrid-cluster protein</fullName>
            <shortName evidence="7">HCP</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Prismane protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">hcp</name>
        <name evidence="8" type="ORF">BN566_01840</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the reduction of hydroxylamine to form NH(3) and H(2)O.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>A + H2O + NH4(+) = AH2 + H(+) + hydroxylamine</text>
            <dbReference type="Rhea" id="RHEA:22052"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15429"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="1.7.99.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="7">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </cofactor>
        <text evidence="7">Binds 1 hybrid [4Fe-2O-2S] cluster.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the HCP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.7.99.1" evidence="7"/>
    <dbReference type="EMBL" id="CBAR010000187">
        <property type="protein sequence ID" value="CCZ84114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01840"/>
    <dbReference type="EnsemblBacteria" id="CCZ84114">
        <property type="protein sequence ID" value="CCZ84114"/>
        <property type="gene ID" value="BN566_01840"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050418">
        <property type="term" value="F:hydroxylamine reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2030">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00069">
        <property type="entry name" value="Hydroxylam_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004137">
        <property type="entry name" value="HCP/CODH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010048">
        <property type="entry name" value="Hydroxylam_reduct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016099">
        <property type="entry name" value="Prismane-like_a/b-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011254">
        <property type="entry name" value="Prismane-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016100">
        <property type="entry name" value="Prismane_a-bundle"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30109">
        <property type="entry name" value="PTHR30109"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30109:SF0">
        <property type="entry name" value="PTHR30109:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03063">
        <property type="entry name" value="Prismane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000076">
        <property type="entry name" value="HCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56821">
        <property type="entry name" value="SSF56821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01703">
        <property type="entry name" value="hybrid_clust"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7" id="KW-0408">Iron</keyword>
    <keyword evidence="6 7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="7">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" description="via persulfide group" evidence="7">
        <location>
            <position position="388"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="416"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="441"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="475"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="477"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Cysteine persulfide" evidence="7">
        <location>
            <position position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ84114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="534" mass="58118" checksum="C5A8A635CCBC14D8" modified="2013-07-24" version="1">MEQKMFCYQCEQTAGCTGCTGSRGVCGKTAQTAQLQDELTGALIGLARATVGDTLATEETWNVMITGLFATLTNVSFDDAAIREQISAVHRAKDALVPQCQSCGAPCGRNDDYDLRQLWEEPDEDIRSLKSLILFGLRGMAAYAYHAMVLGYRDAVIDTFFSEALCKVGETFPMEELLQTALKVGEVNLQCMALLDKANTETFGTPSPKTVSMKIEKGPFIVVTGHDLRDLQLLLEQTKDKGISIYTHGEMLPAHAYPKLNAYPHLKGNFGTAWQNQQKEFADLPAPILFTTNCLMPPKGSYADRVFTTEAVAFPDAVHIGEEKDFTPLIEKALELGGYSEDRQMTGINGGTTVTTGFGHGTILSVAEQVVAGVRSGAIRHFFLVGGCDGAKTGRNYYTEFVKQTPKDSIVLTLACGKYRFNDLDLGTIGGLPRLMDMGQCNDAYGAIQVAVALSQAFGCSVNELPLSYVLSWYEQKAVCILLTLLHLGIQNIRLGPSLPAFLSPNVLQYLVEHYGIAPITTPEADLKALLREA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>R5V860</accession>
    <name>R5V860_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Mannan endo-1 4-beta-mannosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN566_00232</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 26 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000213">
        <property type="protein sequence ID" value="CCZ84319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00232"/>
    <dbReference type="EnsemblBacteria" id="CCZ84319">
        <property type="protein sequence ID" value="CCZ84319"/>
        <property type="gene ID" value="BN566_00232"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016985">
        <property type="term" value="F:mannan endo-1,4-beta-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006080">
        <property type="term" value="P:substituted mannan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005084">
        <property type="entry name" value="CMB_fam6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002105">
        <property type="entry name" value="Dockerin_1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022790">
        <property type="entry name" value="GH26_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000805">
        <property type="entry name" value="Glyco_hydro_26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40079">
        <property type="entry name" value="PTHR40079"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16990">
        <property type="entry name" value="CBM_35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00404">
        <property type="entry name" value="Dockerin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02156">
        <property type="entry name" value="Glyco_hydro_26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00739">
        <property type="entry name" value="GLHYDRLASE26"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51175">
        <property type="entry name" value="CBM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51764">
        <property type="entry name" value="GH26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004406802" evidence="5">
        <location>
            <begin position="32"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="domain" description="CBM6" evidence="6">
        <location>
            <begin position="39"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="GH26" evidence="7">
        <location>
            <begin position="196"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="8">
        <location>
            <begin position="531"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="364"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ84319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="594" mass="66436" checksum="ADCFDDC5E59D363A" modified="2013-07-24" version="1">MSFSHSSRIAAMLLAGSCTLAGIPAVPTVAAETASTAPIRYEFENGTTSGGKIYQETWKGNTEEDGSGEDYDLTNASGGGFSYLDQKGTTVSLEVTVPEDGLYELTVCYCEPYDPNKKVQYLDVNGVNQGEVSFSHNLTFEETSGGVVMLQAGKNTITFRAYWGYTMFDYLEIAPASESLTNLQPTRQLSNPNASDSTKRLYQYLCDQYGKHIISGQQEYCGSHNYNLYADPTNFIKDNEAEFEYLEKTTGKMCAIRGIDFLNYFDGATWDDNAAERAVQWVNEYKGIATIVWHWRVPSEKGGTERAFYVPSASADSTTFSITNALQEGTWEHDVIMADIAEIAKQLKKLKDADVPVLWRPLHEAEGAWFWWGAEGAEPCKQLYRLLYDQLTNVYGLDNLIWVWNSYTYSTSPDWYPGDDVVDIVGYDKYNAADGQPNLSSISSTFYSLVQSTDGEKMVTMAENDTIPSLENLLKDKASWLYFCPWYMNYLTSEQNNPAENLKEIYNSEYCITLDELPDLKSYPLDGGTSPEILLGDINQDGSVNTADVVLLQKYLIQKATLTKAQGTAADYQKDAVIDVLDLLALKRNVWKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5V879</accession>
    <name>R5V879_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative RNA polymerase sigma 28 subunit FliA/WhiG subfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00247</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000215">
        <property type="protein sequence ID" value="CCZ84334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84334">
        <property type="protein sequence ID" value="CCZ84334"/>
        <property type="gene ID" value="BN566_00247"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007630">
        <property type="entry name" value="RNA_pol_sigma70_r4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04545">
        <property type="entry name" value="Sigma70_r4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sigma70_r4" evidence="1">
        <location>
            <begin position="34"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="99" mass="11804" checksum="661714590CC29A45" modified="2013-07-24" version="1">MNHKRLHLPMELITDSHSQEQKANQWLDFLMENCLTARQKQILVLYYYEKQTLREIADQLGITESTACRTKNRACRRLSHYAYPNSIRKELSNHEDPDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5V891</accession>
    <name>R5V891_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Oxidoreductase aldo/keto reductase family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00257</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000217">
        <property type="protein sequence ID" value="CCZ84344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00257"/>
    <dbReference type="EnsemblBacteria" id="CCZ84344">
        <property type="protein sequence ID" value="CCZ84344"/>
        <property type="gene ID" value="BN566_00257"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NADP_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="SSF51430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldo_ket_red" evidence="1">
        <location>
            <begin position="15"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="101" mass="11170" checksum="2BC7D90C5C5D391E" modified="2013-07-24" version="1">MEYTKLGNTDITISKICVGCMSFGKAGIMHDWTLDEAETETVVKHALDLGINFFDTANCYSAGTSEHYLGQALKKNIARDKVVLASKVYFNPGRLSKEAIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5V8P1</accession>
    <name>R5V8P1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative Phosphate-binding protein PstS 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00373</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000238">
        <property type="protein sequence ID" value="CCZ84474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00373"/>
    <dbReference type="EnsemblBacteria" id="CCZ84474">
        <property type="protein sequence ID" value="CCZ84474"/>
        <property type="gene ID" value="BN566_00373"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024370">
        <property type="entry name" value="PBP_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12849">
        <property type="entry name" value="PBP_like_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004395153" evidence="1">
        <location>
            <begin position="33"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="PBP_domain" evidence="2">
        <location>
            <begin position="30"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="PBP_domain" evidence="2">
        <location>
            <begin position="176"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="293" mass="29973" checksum="B7C096CB0C66506F" modified="2013-07-24" version="1">MMKMNWKKTVGMLAGMAVLGAALTGCGNSAGGATGAISVVSREDGSGTRGAFVELCGVEDADGNDATVSSAEITNSTAVMMQTVEGNASAIGYISMGSLGKNDKIKAVQINGVDATPANVSNGSYVVSRPFNIVTKSDGVSDAAQDFINYILSDEGQAIVEQEDFIKTDATGAYKSNGTTGKTISVAGSSSVTPVMQVLAEKYEAVSGNKVEVNQSDSSTGVSSAIDGTCDIGMASRELKDSETSQGVVSTVIAVDGIAVIVNSENTVTNLTLDQVGQIYKGELTEWSQVTAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5V923</accession>
    <name>R5V923_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00093</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000252">
        <property type="protein sequence ID" value="CCZ84599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84599">
        <property type="protein sequence ID" value="CCZ84599"/>
        <property type="gene ID" value="BN566_00093"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="43" mass="4944" checksum="32C7C651115012CC" modified="2013-07-24" version="1">MNGQEPLATCWGILSFYRNIADGQPIIKSRKGKQPFYETHFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5VHC0</accession>
    <name>R5VHC0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00741</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000029">
        <property type="protein sequence ID" value="CCZ82941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82941">
        <property type="protein sequence ID" value="CCZ82941"/>
        <property type="gene ID" value="BN566_00741"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="428" mass="47760" checksum="188DBA2ECBC3056C" modified="2013-07-24" version="1">MKQSLYDIFDHFTPEDNTLPHLKEQPMNEKAISDQVFSKIGYHPIVKTKKKKSYRLFGIAAAVAILACGTITTAAVTGNMDLFFSTVSSSNLSDSGIHLPGVSSSIPDSIAQMQAYYRCPDVTFTATDQATVSLLGFYHDHNTLMLSVQLTVKDDTALTESMGLLPYFTLKTADGTEKALTNSGSLSSYLQKSKTADHVYYATYYLVDSDFSDSTLQVSFSGVYDQNQEQQVQNEIVDLQDHWRTAYSSETTPVEEWKDYWQTHHLDEKTRDARKAAFEKLPAVLDGAWSSEIPIDSIDTTVINAESEGIAISMDELSLSVSQIPETIQQLGAVSPVVYLKDGTILSDDFFMFPDLPKNQDGSVLLTDTPYRQFAFVQGTGTGEDKKTIYCYNQPIDPASIEKVEIYVQYYDADWNKQTDCYSVYSAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>R5VHP3</accession>
    <name>R5VHP3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GTPase Obg</fullName>
            <ecNumber evidence="6">3.6.5.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">GTP-binding protein Obg</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">obg</name>
        <name evidence="10" type="ORF">BN566_00809</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">An essential GTPase which binds GTP, GDP and possibly (p)ppGpp with moderate affinity, with high nucleotide exchange rates and a fairly low GTP hydrolysis rate. Plays a role in control of the cell cycle, stress response, ribosome biogenesis and in those bacteria that undergo differentiation, in morphogenesis control.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="6">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.-" evidence="6"/>
    <dbReference type="EMBL" id="CBAR010000038">
        <property type="protein sequence ID" value="CCZ83016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00809"/>
    <dbReference type="EnsemblBacteria" id="CCZ83016">
        <property type="protein sequence ID" value="CCZ83016"/>
        <property type="gene ID" value="BN566_00809"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01898">
        <property type="entry name" value="Obg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.210.12">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01454">
        <property type="entry name" value="GTPase_Obg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014100">
        <property type="entry name" value="GTP-bd_Obg/CgtA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036346">
        <property type="entry name" value="GTP-bd_prot_GTP1/OBG_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006074">
        <property type="entry name" value="GTP1-OBG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006169">
        <property type="entry name" value="GTP1_OBG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036726">
        <property type="entry name" value="GTP1_OBG_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045086">
        <property type="entry name" value="OBG_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015349">
        <property type="entry name" value="OCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11702">
        <property type="entry name" value="PTHR11702"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09269">
        <property type="entry name" value="DUF1967"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01018">
        <property type="entry name" value="GTP1_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102741">
        <property type="entry name" value="SSF102741"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82051">
        <property type="entry name" value="SSF82051"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02729">
        <property type="entry name" value="Obg_CgtA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03595">
        <property type="entry name" value="Obg_CgtA_exten"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00905">
        <property type="entry name" value="GTP1_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51883">
        <property type="entry name" value="OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51881">
        <property type="entry name" value="OCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Obg" evidence="9">
        <location>
            <begin position="1"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="OBG-type G" evidence="7">
        <location>
            <begin position="158"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="OCT" evidence="8">
        <location>
            <begin position="348"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="164"/>
            <end position="171"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="189"/>
            <end position="193"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="211"/>
            <end position="214"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="281"/>
            <end position="284"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="310"/>
            <end position="312"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ83016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="425" mass="46174" checksum="92DAC3FA094CE183" modified="2013-07-24" version="1">MFVDKVKIYIKAGDGGDGAVSFHREKYVAAGGPDGGNGGKGGNIVFQVDTNLSTLLDFRYHKKYVAKRGENGGAKRCTGKSAPDLVIKVPRGTIVRNAKNGRIMADLSTDEPQILAKGGNGGRGNCNFATSTRQIPRFAKPGFPGEEFEVILELKLLADVGLVGYPNVGKSTLISVVSAAKPKIANYHFTTLTPVLGVVQCNAESSFVMADIPGLIEGASDGVGLGHAFLRHVERCRLILHVVDVSGSEGRDPIADYETIQQELERFNADLAARPQMVAANKCDMATEEQIAAFRNYIEQEKGLPFFCISAATTQGTKELVQAIGAKLTELPPILQYEPEPLTAEELAETTEKGTFEIEVEDGVYYIDAKFLEPILRTVNLDDYSSLQYFQKVLRSSGIIDKLEEMGIQEGDTVNLYGFEFDFVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>R5VHU1</accession>
    <name>R5VHU1_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">Heme chaperone HemW</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN566_00864</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Probably acts as a heme chaperone, transferring heme to an unknown acceptor. Binds one molecule of heme per monomer, possibly covalently. Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the anaerobic coproporphyrinogen-III oxidase family. HemW subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000051">
        <property type="protein sequence ID" value="CCZ83076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00864"/>
    <dbReference type="EnsemblBacteria" id="CCZ83076">
        <property type="protein sequence ID" value="CCZ83076"/>
        <property type="gene ID" value="BN566_00864"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004109">
        <property type="term" value="F:coproporphyrinogen oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006779">
        <property type="term" value="P:porphyrin-containing compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034505">
        <property type="entry name" value="Coproporphyrinogen-III_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010723">
        <property type="entry name" value="HemN_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004559">
        <property type="entry name" value="HemW-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13932">
        <property type="entry name" value="PTHR13932"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06969">
        <property type="entry name" value="HemN_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00562">
        <property type="entry name" value="HemN-like__clustered_with_heat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00539">
        <property type="entry name" value="hemN_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="8 9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 9" id="KW-0349">Heme</keyword>
    <keyword evidence="6 9" id="KW-0408">Iron</keyword>
    <keyword evidence="7 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="10">
        <location>
            <begin position="1"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ83076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="361" mass="40098" checksum="B0C736EF19D2DCF3" modified="2013-07-24" version="1">MPSESTLGIYIHVPFCVRKCSYCDFYSVPQTAERMEQYGTALCRFLRQMQTDLPVDTIYFGGGTPSLLPVSLLEEILQTIAGTVSLRQTEITLECNPATVDRKTLQQLREIGINRLSIGVQSLSDVQLKRLGRLHDAQTAIETVEQAAAVGFQNLSCDVMLALPKQTMAELQGTLTQLTALPIQHISAYLLKVEPETPFASMNWVQQLPDEDATADFYLQTVETLQAAGFLQYEISNFAKAGYESRHNCKYWNCEPYLGFGASAHSCYGGKRLYVPSDLQAFCAAATQPTVLEDDNPCTREEQILLGLRLRKGIPCSWLHTEQLQKLQRYAAGGLVEFLNDRVALTPQGCLVSNAILAEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5VI19</accession>
    <name>R5VI19_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 12">Anaerobic ribonucleoside-triphosphate reductase-activating protein</fullName>
            <ecNumber evidence="12">1.97.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN566_00984</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 12">Activation of anaerobic ribonucleoside-triphosphate reductase under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>glycyl-[protein] + reduced [flavodoxin] + S-adenosyl-L-methionine = 5'-deoxyadenosine + glycin-2-yl radical-[protein] + H(+) + L-methionine + semiquinone [flavodoxin]</text>
            <dbReference type="Rhea" id="RHEA:61976"/>
            <dbReference type="Rhea" id="RHEA-COMP:10622"/>
            <dbReference type="Rhea" id="RHEA-COMP:14480"/>
            <dbReference type="Rhea" id="RHEA-COMP:15993"/>
            <dbReference type="Rhea" id="RHEA-COMP:15994"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:29947"/>
            <dbReference type="ChEBI" id="CHEBI:32722"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:140311"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the organic radical-activating enzymes family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.97.1.-" evidence="12"/>
    <dbReference type="EMBL" id="CBAR010000070">
        <property type="protein sequence ID" value="CCZ83201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00984"/>
    <dbReference type="EnsemblBacteria" id="CCZ83201">
        <property type="protein sequence ID" value="CCZ83201"/>
        <property type="gene ID" value="BN566_00984"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043365">
        <property type="term" value="F:[formate-C-acetyltransferase]-activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012837">
        <property type="entry name" value="NrdG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034457">
        <property type="entry name" value="Organic_radical-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001989">
        <property type="entry name" value="Radical_activat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30352">
        <property type="entry name" value="PTHR30352"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30352:SF2">
        <property type="entry name" value="PTHR30352:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000368">
        <property type="entry name" value="NrdG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00299">
        <property type="entry name" value="anaerobic_ribonucleoside-triph"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02491">
        <property type="entry name" value="NrdG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01087">
        <property type="entry name" value="RADICAL_ACTIVATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="13">
        <location>
            <begin position="23"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR000368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CCZ83201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="167" mass="17935" checksum="9C11E0FD9792F729" modified="2013-07-24" version="1">MQLRIAGTVENSIVDGEGIRYTIFVQGCPHGCPGCHNPQTHDFQGGKLIDAAVLLKEIASDPLLDGVTFSGGEPFCQAAALAALGKQVHDLGLNLVTYTGYTLEQLLADGREDWMALLNETDILIDGRFEADKKSYECRFRGSTNQRYLDAKASVAAGKAVAAPFDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5VI33</accession>
    <name>R5VI33_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00999</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000074">
        <property type="protein sequence ID" value="CCZ83216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00999"/>
    <dbReference type="EnsemblBacteria" id="CCZ83216">
        <property type="protein sequence ID" value="CCZ83216"/>
        <property type="gene ID" value="BN566_00999"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035959">
        <property type="entry name" value="RutC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006175">
        <property type="entry name" value="YjgF/YER057c/UK114"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01042">
        <property type="entry name" value="Ribonuc_L-PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55298">
        <property type="entry name" value="SSF55298"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="133" mass="14819" checksum="5BAB8F36DDCB1474" modified="2013-07-24" version="1">MEFQRVNISGGSSFEEVAGYSRIVKADPFVFVSGTTAVTPEGTVYGEGDPYAQAKYIFTKLVKLLEENGVSKEEVVKVNIWATSSSYNMEITKAYSEFFKESRPCCTWIGIKELNRPTQLVEIEMQALIGAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJC6</accession>
    <name>R5VJC6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00153</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000146">
        <property type="protein sequence ID" value="CCZ83626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00153"/>
    <dbReference type="EnsemblBacteria" id="CCZ83626">
        <property type="protein sequence ID" value="CCZ83626"/>
        <property type="gene ID" value="BN566_00153"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025584">
        <property type="entry name" value="Cthe_2159"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14262">
        <property type="entry name" value="Cthe_2159"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004395176" evidence="2">
        <location>
            <begin position="36"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="317"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="500"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="694"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="370"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="517"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="694"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="753"/>
            <end position="777"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="777" mass="78901" checksum="7AD94280EE1AF374" modified="2013-07-24" version="1">MSSREQNRAWAMTMAVAAVLAYTTGCTDNNSSSTAESTQTTAAISELESESTTTDSEHSESTVATPLSDSEQRVDYDDDDKTSTYDTFDAEITLSGTTADVTGNTNAVSVSDRTVTIQNGGTYRITGTWKDGQLRVTGSEKVKLYFDGVSITNSNGAALSCENEKRTILSLAANTENFLSDGANSNVTTDGETAALFTEDKLTINGSGALTVTGNAANGIVCRDDLKITDGTISVTAANNGMKGKDSIGICGGTITIDAQNDGLKATKADDPEQGWIAISGGTLNITAKGDGIQAETDCYIDGGTLNVTTTGEIAVSTSDDQPFGGGGFGGRFDNGQSPFDGQTPPQGGNRPDGAAPMEKPDDATGSQPAETAPAVTTTTTTTNTEATTDTDTNTTAAEDASSKGIKAGNALTITGGTIQANTTDHCMHSGGAFTCSGGTLELSSSMAMGISSHGDLEITDGSITIDQATEGIESKADMNLAGGTIRILQATDDGLNTGGGTGMGMGVPMGQPDENQPGDPSATTAADETTTTETTTTTTTLSSTDTATDAAEESHELTISGGTIYICADGDGIDSNGSITMTGGTVFVSGPVSGGDGAIDFETDMTMTGGSILALSSRGMMEYPSTQHYYTTSISANAGDFIAVCDADGNVLMGMQTQKAVSDVVYYGDQMDNCQLIVDGTYSGTLSDDGTATDGTVSGGTAADSFQLTDGSEQSSAPGNFGGKGGTPPNNGEMPTFSDGEQPEPPQGEFGKGQQNGENGAPTPLQGSQSSENTTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJD5</accession>
    <name>R5VJD5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized homolog of PSP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00163</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000146">
        <property type="protein sequence ID" value="CCZ83636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00163"/>
    <dbReference type="EnsemblBacteria" id="CCZ83636">
        <property type="protein sequence ID" value="CCZ83636"/>
        <property type="gene ID" value="BN566_00163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007557">
        <property type="entry name" value="PSP1_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04468">
        <property type="entry name" value="PSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51411">
        <property type="entry name" value="PSP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PSP1 C-terminal" evidence="2">
        <location>
            <begin position="61"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="263"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="285" mass="32189" checksum="2B01FA7C0F398067" modified="2013-07-24" version="1">MIEIIGVRFKANGKVYYFSPGDHQFQTGDLVVVETSKGTECGEITIPNKTIQQAAVVQPLKQILRPVNKEDRKTLEQNRKKEIRAFHICEEKIAMRKLEMKLIEVECAFDNSKLLFYFTAENRVDFRDLVKDLAAIFRTRIELRQIGVRDKAKILGGIGICGREFCCKGFLCEFQPVSIKMAKEQGLSLNPAKISGCCGRLMCCLKHEQNVYEELLKITPKLNAMVVLPDGKVGKVVESNTLTGDLKVQVPDNDVPVKCNRSEVKLAKKNHPRSEESKATKSKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJL4</accession>
    <name>R5VJL4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sucrose 6(F)-phosphate phosphorylase</fullName>
            <ecNumber evidence="4">2.4.1.329</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_01466</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>phosphate + sucrose 6(F)-phosphate = alpha-D-glucose 1-phosphate + beta-D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:38863"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:57723"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="EC" id="2.4.1.329"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 13 family. Sucrose phosphorylase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.329" evidence="4"/>
    <dbReference type="EMBL" id="CBAR010000158">
        <property type="protein sequence ID" value="CCZ83731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01466"/>
    <dbReference type="EnsemblBacteria" id="CCZ83731">
        <property type="protein sequence ID" value="CCZ83731"/>
        <property type="gene ID" value="BN566_01466"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004645">
        <property type="term" value="F:1,4-alpha-oligoglucan phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044262">
        <property type="term" value="P:cellular carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11355">
        <property type="entry name" value="AmyAc_Sucrose_phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.400.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="Glyco_hydro_13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045857">
        <property type="entry name" value="O16G_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016377">
        <property type="entry name" value="Sucrose_GGa_phosphorylase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022527">
        <property type="entry name" value="Sucrose_phospho"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003059">
        <property type="entry name" value="Sucrose_phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03852">
        <property type="entry name" value="sucrose_gtfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aamy" evidence="7">
        <location>
            <begin position="6"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="193"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="234"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>sucrose</name>
            <dbReference type="ChEBI" id="CHEBI:17992"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>sucrose</name>
            <dbReference type="ChEBI" id="CHEBI:17992"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="191"/>
            <end position="193"/>
        </location>
        <ligand>
            <name>sucrose</name>
            <dbReference type="ChEBI" id="CHEBI:17992"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>sucrose</name>
            <dbReference type="ChEBI" id="CHEBI:17992"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="291"/>
            <end position="292"/>
        </location>
        <ligand>
            <name>sucrose</name>
            <dbReference type="ChEBI" id="CHEBI:17992"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="335"/>
            <end position="338"/>
        </location>
        <ligand>
            <name>sucrose</name>
            <dbReference type="ChEBI" id="CHEBI:17992"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="392"/>
        </location>
        <ligand>
            <name>sucrose</name>
            <dbReference type="ChEBI" id="CHEBI:17992"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR003059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR003059-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR003059-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="470" mass="54468" checksum="F4790F3253165168" modified="2013-07-24" version="1">MQLKNEIMLITYADSMGNNLKDLHKVLNQYYAKAVGGVHILPFFPSSADRGFAPMCYDRVDPNFGTFADVEAIGKDYYLMFDFMVNHISAHSAYFQDFLQKKDQSAYKDFFIRYSDFWENGEPTQEQVDLIYKRKPRAPYIEAEFADGSKEKVWCTFCEEQIDLDVRKDATKQFIKQTLVDMCHHGAAVIRLDAFAYAIKKPDTSCFFVEPDIWELLYDIEKTVKAEGAEILPEIHEHYTIPMKIADKGFWIYDFALPVLTLHALYNHTGVYLKQWMERCPMHQFTTLDTHDGIGIVDVKDLLPDEEVDKVKEQMYQQGANVKKIYSSEAYNNLDIYQVNTTYYSALGNQDDAYLLARAIQFFAPGIPQVYYVGMLAGSNDVDLMERTKNGRDINRHYYSLEEVAEQQQRPVVQKLKELMELRNTHPAFSLEGTISIQAEGDHLQIVRTYQSHKIVLDANLSTYQFEIHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>R5VK72</accession>
    <name>R5VK72_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Tryptophan synthase beta chain</fullName>
            <ecNumber evidence="11">4.2.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">trpB</name>
        <name evidence="13" type="ORF">BN566_01660</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">The beta subunit is responsible for the synthesis of L-tryptophan from indole and L-serine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + H2O + L-tryptophan</text>
            <dbReference type="Rhea" id="RHEA:10532"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.2.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 11">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 11">Tetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the TrpB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.20" evidence="11"/>
    <dbReference type="EMBL" id="CBAR010000173">
        <property type="protein sequence ID" value="CCZ83931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01660"/>
    <dbReference type="EnsemblBacteria" id="CCZ83931">
        <property type="protein sequence ID" value="CCZ83931"/>
        <property type="gene ID" value="BN566_01660"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00044"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004834">
        <property type="term" value="F:tryptophan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06446">
        <property type="entry name" value="Trp-synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00133">
        <property type="entry name" value="Trp_synth_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006653">
        <property type="entry name" value="Trp_synth_b_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006654">
        <property type="entry name" value="Trp_synth_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023026">
        <property type="entry name" value="Trp_synth_beta/beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001413">
        <property type="entry name" value="Trp_syn_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00263">
        <property type="entry name" value="trpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00168">
        <property type="entry name" value="TRP_SYNTHASE_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9 11" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="10 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 11" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="PALP" evidence="12">
        <location>
            <begin position="52"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="11">
        <location>
            <position position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CCZ83931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="395" mass="43374" checksum="4D2C392A2DE2715E" modified="2013-07-24" version="1">MGKVGRYGMFGGQYVPETVMNAVHELEAAYEEAKADPTFWEEYRTLCRDYANRPSLLYYAERMTKDLGGCKIYLKREDLNHTGAHKINNVLGQILLAKRMGKKRIIAETGAGQHGVATATACALFDLECEVFMGKEDVERQKLNAYRMELLGAKVRSVDNGTGTLKDAINEAMRDWVTNIDTTYYLIGSVMGPHPYPMMVRDFQKIIGEEAKQQLQEKEGVLPDCVVACVGGGSNAMGMFYDFIPNESVRLVGAEAAGKGVDTALHAATIAKGKLGIFHGMKSYFLQNDEGQIAPVFSISAGLDYPGIGPEHANLYQTGRADYVAITDEEAVQALEYLSKKEGVIPAIESAHAVAAAMKIAPTMRKDQIMIICLSGRGDKDMQQIAQYRGVKIDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5VKD7</accession>
    <name>R5VKD7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cellulase M and related proteins</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN566_01710</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="8">Binds 2 divalent metal cations per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the peptidase M42 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000178">
        <property type="protein sequence ID" value="CCZ83981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01710"/>
    <dbReference type="EnsemblBacteria" id="CCZ83981">
        <property type="protein sequence ID" value="CCZ83981"/>
        <property type="gene ID" value="BN566_01710"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008007">
        <property type="entry name" value="Peptidase_M42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023367">
        <property type="entry name" value="Peptidase_M42_dom2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05343">
        <property type="entry name" value="Peptidase_M42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001123">
        <property type="entry name" value="PepA_GA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="204"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="311"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR001123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001123-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001123-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ83981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="339" mass="35806" checksum="F1FF950F43EE6420" modified="2013-07-24" version="1">MKITALLRKLTAIPGVSGREAVVSQPLLELLKEYCPDAALVQGNIIGTFGKRESEKPHVLLDAHFDQVGLIVTYIDENGFLKVGNLGGLDLRLFPAQTVTVHGTSDIPGVVCAMPPHLLAGDADVADITDILIDTGYSKEELEKIVSLGDMISFDAPFEELCGDRICSPALDDRCGIAAILYALSQLDAETLPCSVSVLFSAQEELGERGAKIAAYTIQPDIALAVDVSFGRANEEAPEKCGSLGGGAMIGISPSLSAEVSRDLKNVAETEQIPWQPEVMAGTTGTNADQFSVTRGGVKACTVSIPLRYMHTPAEVIALSDVTATGDLLAAYLRGCKTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5VKR7</accession>
    <name>R5VKR7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Flavodoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_01837</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Low-potential electron donor to a number of redox enzymes.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 3">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the flavodoxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000187">
        <property type="protein sequence ID" value="CCZ84111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01837"/>
    <dbReference type="EnsemblBacteria" id="CCZ84111">
        <property type="protein sequence ID" value="CCZ84111"/>
        <property type="gene ID" value="BN566_01837"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010087">
        <property type="entry name" value="Flav_short"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001226">
        <property type="entry name" value="Flavodoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01753">
        <property type="entry name" value="flav_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00201">
        <property type="entry name" value="FLAVODOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0249">Electron transport</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="4">
        <location>
            <begin position="3"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU367037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ84111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="135" mass="14476" checksum="F07CE89129589727" modified="2013-07-24" version="1">MKAAVIYWSGTGNTEQMANAIAEGAGAELFSVSDFTGDIADYDRIAFGCSAMGDEVLEESEFEPFFTAIEGALSGKTIALFGSYGWGDGEWMRNWEERAKADGAVLVNGEGLIVNETPSDEDLETCKELGKQLIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5VKW6</accession>
    <name>R5VKW6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Kinase PfkB family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01896</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000193">
        <property type="protein sequence ID" value="CCZ84171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01896"/>
    <dbReference type="EnsemblBacteria" id="CCZ84171">
        <property type="protein sequence ID" value="CCZ84171"/>
        <property type="gene ID" value="BN566_01896"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016773">
        <property type="term" value="F:phosphotransferase activity, alcohol group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00584">
        <property type="entry name" value="PFKB_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PfkB" evidence="3">
        <location>
            <begin position="3"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="328" mass="36248" checksum="9510DF54E45D9B56" modified="2013-07-24" version="1">MYDVTAIGEALIDFIPAQRDCALKEVESFTRVCGGAPTNVAAVVAKLQGNARLLTQVGNDAFGDYIVDTIQACGVKTDGVCRTDAANTALAFVSLKADGNRDFSFYRKPSADLLLQPEQIQPIWLEETGILHFCSVSLVESPMKETHKAVLKLAKKNHAIISFDPNIRLPLWSSAEACQETVREFLPDADLVKLSDEECFFVTGKHSILDAADWLFEQGCKMVLYSMGKEGAMILTPHFSSHVENPEVRVKDTTGAGDAIIGAYLYCLAKNHITVSQLADCLPEQVQEWLTFAVYYANYSITKSGAIASYPDYTTFVKWMQEKRKKAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>R5VLL5</accession>
    <name>R5VLL5_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein translocase subunit SecD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">secD</name>
        <name evidence="11" type="ORF">BN566_00343</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. SecDF uses the proton motive force (PMF) to complete protein translocation after the ATP-dependent function of SecA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Forms a complex with SecF. Part of the essential Sec protein translocation apparatus which comprises SecA, SecYEG and auxiliary proteins SecDF. Other proteins may also be involved.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the SecD/SecF family. SecD subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000225">
        <property type="protein sequence ID" value="CCZ84436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00343"/>
    <dbReference type="EnsemblBacteria" id="CCZ84436">
        <property type="protein sequence ID" value="CCZ84436"/>
        <property type="gene ID" value="BN566_00343"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015450">
        <property type="term" value="F:protein-transporting ATPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043952">
        <property type="term" value="P:protein transport by the Sec complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01463_B">
        <property type="entry name" value="SecD_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005791">
        <property type="entry name" value="SecD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022813">
        <property type="entry name" value="SecD/SecF_arch_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022645">
        <property type="entry name" value="SecD/SecF_bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30081">
        <property type="entry name" value="PTHR30081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02355">
        <property type="entry name" value="SecD_SecF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00916">
        <property type="entry name" value="2A0604s01"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="7 9" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004395251" description="Protein translocase subunit SecD" evidence="10">
        <location>
            <begin position="36"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="271"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="299"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="328"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="371"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="422"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ84436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="470" mass="50444" checksum="FC97D36048CAA42E" modified="2013-07-24" version="1">MKKVKKPVFFLVFAVIAIFAITTLIGVSTQNGDIATTYVHGVKDIRLGIDIQGGVDVTFEPAGDVDATDEQMDAALEKIKTRLVSQGINDSDTYVDYKSDRIIVRFPWQAGETDFDPEQAVKELGETAELTFRYGTETTTNEDGETVPAGEIVLTGDDVKSAGTGATQDDTTKEATWMVTLDLNDSGKEKFYNATSALYQDNGQISIWMDNTMISAPSVNAVISDGKATISGNFTYESAKELADKINAGALPFALETTSFKTISPTMGEGALNAMVIAGLITCAFIIIYMIALYRLLGVVASLCLIGQITGMLAAVSGWFGFMDSNTLTIPGIAGIILSIGMGVDANIITGERIREELRRGKPLDGALQSGYSRAFSAIFDGNLTGIIVSIILMGAFGTPDSWCAKLLHPLFQFFGATTEGVIYSFGYTLLVGLIMNFIMGVFAARLMTMSLSKFKCFQNKKLYGGGLNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5VQ98</accession>
    <name>R5VQ98_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00562</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000003">
        <property type="protein sequence ID" value="CCZ82757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00562"/>
    <dbReference type="EnsemblBacteria" id="CCZ82757">
        <property type="protein sequence ID" value="CCZ82757"/>
        <property type="gene ID" value="BN566_00562"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007211">
        <property type="entry name" value="DUF378"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37304">
        <property type="entry name" value="PTHR37304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04070">
        <property type="entry name" value="DUF378"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="69" mass="7545" checksum="5CD284E99C346F3B" modified="2013-07-24" version="1">MWDKLALILLVIGGVNWGLVGIFEFDLVAWLFGGATSALSRIVYILVAISAVWCISFLFRREALATDAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5VQP2</accession>
    <name>R5VQP2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Glycosyl hydrolase family 59</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00694</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000022">
        <property type="protein sequence ID" value="CCZ82892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00694"/>
    <dbReference type="EnsemblBacteria" id="CCZ82892">
        <property type="protein sequence ID" value="CCZ82892"/>
        <property type="gene ID" value="BN566_00694"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004336">
        <property type="term" value="F:galactosylceramidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006683">
        <property type="term" value="P:galactosylceramide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001286">
        <property type="entry name" value="Glyco_hydro_59"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15172">
        <property type="entry name" value="PTHR15172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02057">
        <property type="entry name" value="Glyco_hydro_59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ82892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="785" mass="89473" checksum="D578B6375E356447" modified="2013-07-24" version="1">MDFKWKNGQLFQKFSEMTGKSGVASMEIIADGTQANENPAAAYRGLGCISANNASRLLLDYKVEHPKEYEEIMRLLFQKDYGIGLSHIKLELGADVNSSCGTEPATKRTPEERADVTRGAGFQFAADAKAINPDITLDLLRWGEPAWVARAFTVSQEHGFNARYSWIKETLDAAYRVYGLKFHFISAEQNETDRIDESWILFLRYRLDHEVRAPYDYRKIKLVASDEVGTRNIAAQMVENASLRNAIDVIGLHYTTFGDSYTNLLNEAYGKEIWYSEGSAPCNLSELTVQADQSGLVGKNSAIDIANRIINSYYNGKMCMYEFRPAIAANYDGAYDEPKHLIAAQEPWSGFYRLDSGFWMAMHFSRFSPKGWLFVNGACYGDGEENHAIEHTSHNFMTLVSPDRSEMTMHFTNEDLFPRTYSVQLKDMGFTNRMLSTVITTGPNPGEAFDANWFRRGKPIKPSHRNGETYFIKVPPQSIMTITTLDTGWVNGVETFSHNIPNAERLPLPYQDNFRYQSDEIMVRGCAPRYMTDQGGAFELIHSDQDGDILCQRIKKGNIPANWRYRGTPEPITCLGDDRWRSYSAEIEVRLDNRDAENYAGFGIRYNSTVTCETTSRCGYTGILYGDGRWQLLDMDNVAAEGNSMTVRATDWNKLKLLILGDSIFFFLNGDLLTTYRPQSPVNSGRISICSDYEMNTFRNLQVTPLPILPLYVRRVDCFSEEIFYNDFWDICTTESYRFYHRNLYHRILSLLSPRFNEGTATGSVLLSVYRNGYRCHWNGKKCGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>R5VR02</accession>
    <name>R5VR02_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator AraC family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00777</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000034">
        <property type="protein sequence ID" value="CCZ82977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00777"/>
    <dbReference type="EnsemblBacteria" id="CCZ82977">
        <property type="protein sequence ID" value="CCZ82977"/>
        <property type="gene ID" value="BN566_00777"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="1">
        <location>
            <begin position="158"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="268" mass="31100" checksum="2E76A6D815088ABB" modified="2013-07-24" version="1">MAQQQGLILLQPYLTLEAQHCQRIPQVGTGIAECYEFTIPANGQSQFPAVPDGSVDLVFGMSKQDVRLFIGGTVLQMKQWCFDPGRTYFGVRFQPGKSLLPKDLCIQDVVNADLELQPSVYGEGLAEKIATADSLQARSQLFLQTYLQQFPESTDTRHNLERYVRRRIYETKGTIGIQNLAEETGYSACYLRRVFEQIHGISPKLFERFIRFQNMLYLMEQQEWQSQKMDALCFESGYYDQSHMMKDFKRFAGRTPEQYRKFRLHPEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>R5VR20</accession>
    <name>R5VR20_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ABC-type Mn/Zn transport system</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_00785</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ABC-3 integral membrane protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000036">
        <property type="protein sequence ID" value="CCZ82992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00785"/>
    <dbReference type="EnsemblBacteria" id="CCZ82992">
        <property type="protein sequence ID" value="CCZ82992"/>
        <property type="gene ID" value="BN566_00785"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001626">
        <property type="entry name" value="ABC_TroCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30477">
        <property type="entry name" value="PTHR30477"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00950">
        <property type="entry name" value="ABC-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81345">
        <property type="entry name" value="SSF81345"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="56"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="92"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="135"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="192"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="247"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ82992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="283" mass="30531" checksum="6511E77E54863A87" modified="2013-07-24" version="1">MTFLIDMLSFAFIRRALLVGIFVSLCAALLGVSLVLKRFSMIGDGLSHVSFGALSVALAFGWAPLKFSIPIVAIAAFFLLRITERAKVKSDAAIAVVSASSLAIGIIVTALKTGINTDVASYMFGSILAMEKEDVWVSLILSIVVLGMFVICYHRIFAITFDENFAQATGVSVSFYNTLIAILTAITVVLGMRMMGALLISSLIIFPALSSMRLFKRFSLVILCAAILSVVCFCVGMVGSYQFDTPVGASVVVANLAAFLLFCLIQKIREQVKKRTIQAQPKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5VR68</accession>
    <name>R5VR68_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00825</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000040">
        <property type="protein sequence ID" value="CCZ83032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00825"/>
    <dbReference type="EnsemblBacteria" id="CCZ83032">
        <property type="protein sequence ID" value="CCZ83032"/>
        <property type="gene ID" value="BN566_00825"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="366" mass="42069" checksum="97C4CA245DC0153B" modified="2013-07-24" version="1">MNSCEQIRTAMMAYDWIGFDLFDTLLLRPFLQPDDLFCQMEQELQVPDFAALRSQTENQLRQGRKRELTFLEIYQAIQQKTGVSDAVIQQWMDLELELEWRYCYPRESGLSLYCAAKEAGKHIAIVTDMYLPIDQIVRMLQKVGAGDYDLLLLSSELGYSKANGGIYRQLKRMEIPPKQFLQIGDNRIADVENPRCLGLQGMWLPAARAVFQHYGRLYPAIQAEQPSLAERCSVALAVNTYFDDPFRMMRRECDWNADPYFMGLLLPNAEHLPEVVTQDFMFQRGQERAMQLATHFFEHPLPAAQGAIEMLCHHAAEADRAAFPKDDAWAAVCTPCGFSTLPEPKKPAKWKTHLRLLRQFLSTCRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5VR83</accession>
    <name>R5VR83_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0291 protein BN566_00111</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00111</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0291 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000046">
        <property type="protein sequence ID" value="CCZ83057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00111"/>
    <dbReference type="EnsemblBacteria" id="CCZ83057">
        <property type="protein sequence ID" value="CCZ83057"/>
        <property type="gene ID" value="BN566_00111"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01103">
        <property type="entry name" value="UPF0291"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009242">
        <property type="entry name" value="DUF896"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37300">
        <property type="entry name" value="PTHR37300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05979">
        <property type="entry name" value="DUF896"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="70" mass="8047" checksum="8778B6F4FE0E6B4D" modified="2013-07-24" version="1">MTPEKIARINELAKKQKAGTLTDAEKEEQAALRLEYRQSVVGNLRQQLNNVEFVEPDGKKTRPCHNKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5VRP4</accession>
    <name>R5VRP4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sporulation transcriptional activator Spo0A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01008</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000080">
        <property type="protein sequence ID" value="CCZ83227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01008"/>
    <dbReference type="EnsemblBacteria" id="CCZ83227">
        <property type="protein sequence ID" value="CCZ83227"/>
        <property type="gene ID" value="BN566_01008"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042173">
        <property type="term" value="P:regulation of sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014879">
        <property type="entry name" value="Spo0A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08769">
        <property type="entry name" value="Spo0A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spo0A_C" evidence="2">
        <location>
            <begin position="15"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="114" mass="12842" checksum="8E0C9CEA1BA23D84" modified="2013-07-24" version="1">MNKADKETLSAIIQTQIGVSKKLKGYQYLEDAVEIALQNPQMPVMQIYQLLGNQHGRSAVSIERTLRYAIETAYDKTQLNGSFPNSGGKPSNSEVIWTIAEEIKRVRHYACRKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5VS14</accession>
    <name>R5VS14_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized conserved protein UCP037246</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00124</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000090">
        <property type="protein sequence ID" value="CCZ83342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00124"/>
    <dbReference type="EnsemblBacteria" id="CCZ83342">
        <property type="protein sequence ID" value="CCZ83342"/>
        <property type="gene ID" value="BN566_00124"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017154">
        <property type="entry name" value="PC4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003173">
        <property type="entry name" value="PC4_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02229">
        <property type="entry name" value="PC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037246">
        <property type="entry name" value="UCP037246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PC4" evidence="1">
        <location>
            <begin position="20"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="77" mass="8846" checksum="2F90347F0D6DC194" modified="2013-07-24" version="1">MAELKYEIVEHIGVLSENARGWRKELNLVSWNDREPKYDIRDWAPEHAKMGKGVTLSADEAVELKNLLAGIDFDSFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5VS86</accession>
    <name>R5VS86_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ribosomal protein S1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01178</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000095">
        <property type="protein sequence ID" value="CCZ83402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01178"/>
    <dbReference type="EnsemblBacteria" id="CCZ83402">
        <property type="protein sequence ID" value="CCZ83402"/>
        <property type="gene ID" value="BN566_01178"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035104">
        <property type="entry name" value="Ribosomal_protein_S1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10724:SF7">
        <property type="entry name" value="PTHR10724:SF7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="S1 motif" evidence="1">
        <location>
            <begin position="126"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="1">
        <location>
            <begin position="209"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="309" mass="33904" checksum="F2FA0F60FBCD2A8A" modified="2013-07-24" version="1">MTTYLPEGMRLHTMENQTALQSVAALQAAMHRKQILEARAIVCDAAHNLIVELPHMRGIIPREEGAIGIAEGTTREIALIAKAGKPVCFQVQELTTDADGVPLAILSRRQAQLQCWQEFLSHCTPGDVLPARVTHMEKFGCFVDIGCGIPSMIPIDCISVSRISHPSDRFTLGQTIQAVVTSVSDFRIGLSHKELLGTWMENAAKFAVGETVSGIVRSVESYGVFVELTPNLAGLAEPKEHISPKQRASVYIKAIIPEKMKIKLVLIDAFPDTESPDALSYFVQEPHLSTWNYSPIGATKQIQTQFDVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>R5VSM1</accession>
    <name>R5VSM1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC-type multidrug transport system ATPase and permease components</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN566_01281</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000119">
        <property type="protein sequence ID" value="CCZ83512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01281"/>
    <dbReference type="EnsemblBacteria" id="CCZ83512">
        <property type="protein sequence ID" value="CCZ83512"/>
        <property type="gene ID" value="BN566_01281"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="PTHR24221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="250"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="320"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="346"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="426"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="460"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="237"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="523"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="557"/>
            <end position="564"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ83512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="781" mass="85871" checksum="EC85D0B15148DD3D" modified="2013-07-24" version="1">MGKLFKNLKPYWKSVLMIVVLLVVQAFCDLSLPQYTSDIIDTGIQNSGVEHILPEKITAEEFQNAQLFMTADEQKTWQDAYNGDGDILDRNDFSKKDLEQLDSDLIVPILLNYQMSMMSEDGFREMLMQQQQGNPQAAEQLKTMSIADIGNSMGVTLTPFERTEEDDDGNKTTVTCVDVRPLFAAMQEAGQFTEETRSEMRSQLETMLDTMGESLVQSMGIAYAVQCDQAAGLDTDKIQTNYLWAAGGKMIAIAFAMMAVTICISYVAARVGAGVGRDLRKKVFSRVMQFSNAEMDQFSTASLITRSTNDIQQVQMVTAIFLRMLLYAPILGIGGILKVVNTGAGMGWVIVLAVLVILGIVGVLMSIAMPKFKKMQTLVDRVNLVSREILTGLSVIRAFGREKTEEKRFDTANQNLTKTNLFVNRVMTFMMPVMMLIMYSLTILIVWVATHRIDGGSMQVGSMTAFITYAMMIVMSFLMLTMMSVMLPRASVAAGRIDEVLQTKTSVQEPTAPQHLAQPAGVLTFDHVSFRYPHAEEDALSDLSFTAEPGKTTAIIGSTGCGKSTLVNLIPRLYDATSGKITLDGVEIQDLSLEELRQQIGFVPQKGVLFSGTIASNLRFGNPTATDAEIQQAAEIAQASDFIEEKHDRYESTIAQGGSNVSGGQKQRLTIARAIAKHPKIFVFDDSFSALDLKTDAALRKALQEQVQNSTMIIVAQRISTILHADQILVLENGSIVGKGTHEELLHNCTVYQQIAKSQLSAEELGLSESDTEEGAMNDGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VSN1</accession>
    <name>R5VSN1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01296</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000121">
        <property type="protein sequence ID" value="CCZ83527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83527">
        <property type="protein sequence ID" value="CCZ83527"/>
        <property type="gene ID" value="BN566_01296"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="258" mass="30967" checksum="9E14ED12F262CD75" modified="2013-07-24" version="1">MFSIFRFPSPIALFARILFYKYSSIKNKRYGFFSISLVLFSLIETGWNLPCKQVLRIMKRLRHGKHLSMISFLPTQCMIFSTRFVVQNVKDFHPCKCKPIRKTATSHTKQKRYSFHPYRLMFIFCQCRWNGFHRAFSLLESVHRIAPPASSLFEKLLHSRKAKRYSFHPYRLMFIFSQRRRHDCRRAFSLLESVHRIAPPASSLFEKLLHSRKAKRYSFHPYRLMFIFSQRRQHGCHMAFSLPESVHQIALPMRTHEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5VSN9</accession>
    <name>R5VSN9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Peptidase_M50 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">BN566_01306</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">MGS:254</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase M50B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000121">
        <property type="protein sequence ID" value="CCZ83537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01306"/>
    <dbReference type="EnsemblBacteria" id="CCZ83537">
        <property type="protein sequence ID" value="CCZ83537"/>
        <property type="gene ID" value="BN566_01306"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06158">
        <property type="entry name" value="S2P-M50_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008915">
        <property type="entry name" value="Peptidase_M50"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044537">
        <property type="entry name" value="S2P-M50-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02163">
        <property type="entry name" value="Peptidase_M50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="62"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="183"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M50" evidence="15">
        <location>
            <begin position="134"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CCZ83537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="228" mass="25651" checksum="7200F89C02ADC41C" modified="2013-07-24" version="1">MNLLSMSKEQLLTMLIARVIIILLILPLHEFAHAWVARLCGDDTADSKGRLTLNPFSHVDPFGAILLLLTGFGWAKPVPINPNRMRNPRTGVIWTSLAGPASNLLIAFVMMIPFRILENIPISSEAMYNTLSIIYSILYFFILVNIGLAVFNLIPIPPLDGSNILLGFLPYKAIYWVQQHRQVISFVFLILIFSDFLDTPLSYLQQLIFKGFTFLTNWVPLMVSGLTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VSY8</accession>
    <name>R5VSY8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01388</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000147">
        <property type="protein sequence ID" value="CCZ83652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83652">
        <property type="protein sequence ID" value="CCZ83652"/>
        <property type="gene ID" value="BN566_01388"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="46" mass="5272" checksum="4ADE0ABE6E4F9C9C" modified="2013-07-24" version="1">MNGMAEHEPESYVSERTQIRHLRAMRAGLERILAVYRVVCVSGLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>R5VTI7</accession>
    <name>R5VTI7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methylglyoxal synthase</fullName>
            <shortName evidence="1">MGS</shortName>
            <ecNumber evidence="1">4.2.3.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mgsA</name>
        <name evidence="4" type="ORF">BN566_01577</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of methylglyoxal from dihydroxyacetone phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>dihydroxyacetone phosphate = methylglyoxal + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17937"/>
            <dbReference type="ChEBI" id="CHEBI:17158"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="EC" id="4.2.3.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the methylglyoxal synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.3" evidence="1"/>
    <dbReference type="EMBL" id="CBAR010000166">
        <property type="protein sequence ID" value="CCZ83842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01577"/>
    <dbReference type="EnsemblBacteria" id="CCZ83842">
        <property type="protein sequence ID" value="CCZ83842"/>
        <property type="gene ID" value="BN566_01577"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008929">
        <property type="term" value="F:methylglyoxal synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019242">
        <property type="term" value="P:methylglyoxal biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01422">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00549">
        <property type="entry name" value="Methylglyoxal_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004363">
        <property type="entry name" value="Methylgl_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011607">
        <property type="entry name" value="MGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036914">
        <property type="entry name" value="MGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30492">
        <property type="entry name" value="PTHR30492"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02142">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006614">
        <property type="entry name" value="Methylglyox_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00851">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52335">
        <property type="entry name" value="SSF52335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00160">
        <property type="entry name" value="MGSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51855">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MGS-like" evidence="3">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="1">
        <location>
            <begin position="34"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="1">
        <location>
            <begin position="54"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1 2">
        <location>
            <position position="60"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR006614-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="134" mass="14844" checksum="CCAFB3D0F7846738" modified="2013-07-24" version="1">MTIALIAHDAKKELMIQFCIAYCGVLSRHKLCATGTTGKLVSEATGLEIQRYLSGRQGGDQQIAARIACDEIDVLLFFRDPINRKESEPNEMALLRLCDVHNVPVATNIATAEALIMALERGDLDWREYGNLPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5VTN7</accession>
    <name>R5VTN7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01616</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000169">
        <property type="protein sequence ID" value="CCZ83887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83887">
        <property type="protein sequence ID" value="CCZ83887"/>
        <property type="gene ID" value="BN566_01616"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025699">
        <property type="entry name" value="ABC2_memb-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13346">
        <property type="entry name" value="ABC2_membrane_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="18"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="179"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="212" mass="23259" checksum="801BCB1DD574BDB1" modified="2013-07-24" version="1">MQTLLWYRFQTIWKQMKVVLICMGVLAGAVLLFALIGGAAAWEAGSFFFGFFVVFLSIYASMLPIFVLQVEEQEKNTDLLLTLPLTRKQVALGNYVLTWICAAIMGGYALLLAIALQGIWGIVAVAMGIVLLVNTIYLPLFVYFGSQKALIFVLLLMVSGGIGFSSISDQTNGFPFDGGFTMVWIVGILAGLLLLHGVSLLLTLHKYQTKDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5VU93</accession>
    <name>R5VU93_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GIY-YIG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01808</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000186">
        <property type="protein sequence ID" value="CCZ84082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01808"/>
    <dbReference type="EnsemblBacteria" id="CCZ84082">
        <property type="protein sequence ID" value="CCZ84082"/>
        <property type="gene ID" value="BN566_01808"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025579">
        <property type="entry name" value="DUF4357"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000305">
        <property type="entry name" value="GIY-YIG_endonuc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14267">
        <property type="entry name" value="DUF4357"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50164">
        <property type="entry name" value="GIY_YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GIY-YIG" evidence="1">
        <location>
            <begin position="50"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="298" mass="33290" checksum="416B570E9395FC84" modified="2013-07-24" version="1">MKNRGKSINLYLMDGDASGRIKCTLANWTGVAYKIPRTDLDKCKEREDLKQSGVYFLFGTSDETGKNIVYIGQAGARKNGEGILNRLYEHKRNPEKDYWTEAIVFTTSNNSFGPTEISYLENRFCNLALSVNRYEVKNGNDPTPGNITEEKESEMEEFIDYAKIIIRTLGHKLFEPIHQLDTEKTEETTLAPSQVKLLYLERTIKDIGKVKAMGCQTSDGFVVMKGSQISTKDADSIPKTVKEKREKANITGGILQEDILLSSPSYAASFVIGQSCNGLTSWKTKEGQTLKALEAADI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5VUK4</accession>
    <name>R5VUK4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Helix-turn-helix domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01932</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84207">
        <property type="protein sequence ID" value="CCZ84207"/>
        <property type="gene ID" value="BN566_01932"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="7"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="150" mass="16931" checksum="26209C564E3EDD40" modified="2013-07-24" version="1">MFFSDKLRLLRTQANLSQEDLADELHVSRQSVSKWESGISFPEIEKLISISDYFHVSLDALLKAPLHDTLITETMDRLVLQFLGSSQSMHDISQQLVTIMQDGKIDAQEALQLEQILCALDHIMQDIRKLKQTFSTAYTTNTTGISGSES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5VUW5</accession>
    <name>R5VUW5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein conserved in bacteria</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00225</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FldB/FldC dehydratase alpha/beta subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000212">
        <property type="protein sequence ID" value="CCZ84312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00225"/>
    <dbReference type="EnsemblBacteria" id="CCZ84312">
        <property type="protein sequence ID" value="CCZ84312"/>
        <property type="gene ID" value="BN566_00225"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016836">
        <property type="term" value="F:hydro-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010327">
        <property type="entry name" value="FldB/FldC_alpha/beta"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06050">
        <property type="entry name" value="HGD-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="408" mass="46500" checksum="688CDC23765AFC83" modified="2013-07-24" version="1">MQYQQFTPEMRKTHTILVPNMLPIHFRLLISIFGQFGYHMELLESSSRTVVEEGLKNVHNDTCYPALLVVGQFMEALKSGKYDPDKTALLITQTGGGCRASNYIHLLRKCMAQQFPQVPVISLNFAGLEKASGFQLTLPLCIKLVYGVLYADLLMTIYNQCRPYELNEGESQKTLEQWQNKLDKLFRTNDYRHTKKWYRAMLEDFAAIPRSQKPKIRVGIIGEIYVKYSPLANNHLEDFLLQEGCEPVVPTLMSFVLYCAANSENNAKLYQYRNLHTALFGIGYKFLHSKQMETIRCMQEHGVFTPMHDFEELRKAADKYTNQGICMGEGWMITAEMAVLVEHGIKNIICTQPFGCLPNHIVAKGMSRTIKQAYPEANIVAIDYDPGATKVNQENRIKLMLASAAAEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>R5VV52</accession>
    <name>R5VV52_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Aspartokinase</fullName>
            <ecNumber evidence="14">2.7.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">BN566_00299</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the phosphorylation of the beta-carboxyl group of aspartic acid with ATP to yield 4-phospho-L-aspartate, which is involved in the branched biosynthetic pathway leading to the biosynthesis of amino acids threonine, isoleucine and methionine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>ATP + L-aspartate = 4-phospho-L-aspartate + ADP</text>
            <dbReference type="Rhea" id="RHEA:23776"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57535"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 15">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 15">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5 15">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 14">Belongs to the aspartokinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.4" evidence="14"/>
    <dbReference type="EMBL" id="CBAR010000223">
        <property type="protein sequence ID" value="CCZ84392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00299"/>
    <dbReference type="EnsemblBacteria" id="CCZ84392">
        <property type="protein sequence ID" value="CCZ84392"/>
        <property type="gene ID" value="BN566_00299"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00015"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00461"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00462"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004072">
        <property type="term" value="F:aspartate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005260">
        <property type="entry name" value="Asp_kin_monofn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001341">
        <property type="entry name" value="Asp_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018042">
        <property type="entry name" value="Aspartate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027795">
        <property type="entry name" value="CASTOR_ACT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13840">
        <property type="entry name" value="ACT_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000726">
        <property type="entry name" value="Asp_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00657">
        <property type="entry name" value="asp_kinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00324">
        <property type="entry name" value="ASPARTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword evidence="9 14 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="8 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ACT" evidence="16">
        <location>
            <begin position="378"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="5"/>
            <end position="8"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="206"/>
            <end position="207"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00010122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000726-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CCZ84392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="442" mass="48332" checksum="D5BD33C1A640B0D6" modified="2013-07-24" version="1">MKVVKFGGSSLADAGQIQKAAAIIKAEADRRYVVPSAPGKRFKDDIKVTDMLYQCYALAEKGEAFATQLAAIKDRYVSILNGLSMPETLLDEEFVTIGENFRKRFGSDYAASRGEYLNGKLIAAYLGFAFVDAAEVIFFQEDGTLNREKTAVAMRERLTGLDYAVVPGFYGCGADGYVKTFSRGGSDVTGSIVAGAMKADLYENWTDVSGFLVADPRIVENPVGIRTITYTELRELSYMGASVLHEEAIFPVRSAGIPINIRNTNDPSAPGTMIVPECNADDCSTYTITGIAGKKHFSIINIHKDKMNSEVGFCRDVLDVLASHNISIEHMPSSIDAMTVILETAQLEGKREQIMTELRKRVNPDTLEIEDGIALMAVVGHGMYRTRGVAARIFAALAHARVNVKMIDQGSSELNIIVGVTEEDFEQAIRCVYDMFVKSASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5VV64</accession>
    <name>R5VV64_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-propeller domains of methanol dehydrogenase type</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00309</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000224">
        <property type="protein sequence ID" value="CCZ84402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84402">
        <property type="protein sequence ID" value="CCZ84402"/>
        <property type="gene ID" value="BN566_00309"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="261" mass="29203" checksum="4B8550E1A5380FD3" modified="2013-07-24" version="1">MTCPYCGAQQQSKSIYCDNCGQLLPALHTETPVSQKHPFRFWLVSLILLPILLIIGIALYAMHAPQPTEPESSVLPAPQVWNGGTAALLWSEDVIDSEADRQALCNRLQEVSTMIQMPVGIQLSDEPIPDDDTAREQAIADFTEQFPDAADGVFLYLDLTEPEPGQTYNSHDYFLTYGAAQLYYTNAPDYNRIAEIFAQVNAACELEQATTLRRVELFCTALEETYQEGIPDGYYVQDSDTGQYLYESDGEIGWHDTLPEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVM7</accession>
    <name>R5VVM7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DNA ligase</fullName>
            <ecNumber evidence="11">6.5.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Polydeoxyribonucleotide synthase [NAD(+)]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ligA</name>
        <name evidence="13" type="ORF">BN566_00413</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="11">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the NAD-dependent DNA ligase family. LigA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.2" evidence="11"/>
    <dbReference type="EMBL" id="CBAR010000243">
        <property type="protein sequence ID" value="CCZ84552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00413"/>
    <dbReference type="EnsemblBacteria" id="CCZ84552">
        <property type="protein sequence ID" value="CCZ84552"/>
        <property type="gene ID" value="BN566_00413"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003911">
        <property type="term" value="F:DNA ligase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01588">
        <property type="entry name" value="DNA_ligase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041663">
        <property type="entry name" value="DisA/LigA_HHH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033136">
        <property type="entry name" value="DNA_ligase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001679">
        <property type="entry name" value="DNAligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013839">
        <property type="entry name" value="DNAligase_adenylation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013840">
        <property type="entry name" value="DNAligase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003583">
        <property type="entry name" value="Hlx-hairpin-Hlx_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004150">
        <property type="entry name" value="NAD_DNA_ligase_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004149">
        <property type="entry name" value="Znf_DNAligase_C4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01653">
        <property type="entry name" value="DNA_ligase_aden"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03120">
        <property type="entry name" value="DNA_ligase_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03119">
        <property type="entry name" value="DNA_ligase_ZBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12826">
        <property type="entry name" value="HHH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00278">
        <property type="entry name" value="HhH1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00532">
        <property type="entry name" value="LIGANc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="SSF47781"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="SSF52113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00575">
        <property type="entry name" value="dnlj"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01056">
        <property type="entry name" value="DNA_LIGASE_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9 11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 11" id="KW-0235">DNA replication</keyword>
    <keyword evidence="2 11 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 11" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11" id="KW-0520">NAD</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="BRCT" evidence="12">
        <location>
            <begin position="373"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CCZ84552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="455" mass="49726" checksum="342FFEEFA72D504A" modified="2013-07-24" version="1">MTAKRGLDILIFNVQQIDGETLTTHRQSLDYLRQLGMKTVQATEPLTTESEVLDALQAVGAERTDLPYDIDGVVVKVNDFAQREEMGATAKVPKWAVAYKFPPEEKETKLLEILNSVGRTGVITPVAVFEPIRLAGTDVSRATLHNQDFITEKGICLGDTIIVRKAGEIIPEVVRSASHLPDAVPYEMPEVCPVCGTKAVRDAEESALRCPNPDCPAQLLKRMIHFASKDAMNIEGLGAQNVLALQTAGYLHSVADLYRLTKEQLLQLDRFAEKSAENLLQAIAKSKQNPLPRLIFGLGIRNIGLQAAKLLCDAFPSMDAIMQATAEQIAEIEGFGMVMAESVVAAFREPHMQHLIAELQAYGVQMQHTKAVQTDQRFANQTFVLTGTLPTMKRAEAKALIEQFGGKVSGSVSKKTAYVVAGAEAGSKLQKAEELGIPIWTEQDLLAHTKEEPEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVQ0</accession>
    <name>R5VVQ0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00453</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000251">
        <property type="protein sequence ID" value="CCZ84592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84592">
        <property type="protein sequence ID" value="CCZ84592"/>
        <property type="gene ID" value="BN566_00453"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="60" mass="7154" checksum="19C00AAC6FC58DAE" modified="2013-07-24" version="1">MTANGSELFYWHTNKEWYKINIEEGYCELTEKAPPEAVESFKDWNKITKLGRIKEEKNKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVX8</accession>
    <name>R5VVX8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00540</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000267">
        <property type="protein sequence ID" value="CCZ84687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84687">
        <property type="protein sequence ID" value="CCZ84687"/>
        <property type="gene ID" value="BN566_00540"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="261" mass="29007" checksum="CC7535CFACB5CB2E" modified="2013-07-24" version="1">MKQHCPSCGKKFDYEQHGWICPFCGQVILDSTEQTVHQQESATPPPPAKKPSRKLKKLSVLFTVGLLLTVSSFGYRIGKNLIYLRQKQTSEPVSYPVETIEMQQTISIEPYQLTIQQAYLFPQTLITAPKSGEYLAVSYTSSGKSSDSSIQRIDDVAWAALKLEQANQYITPLSLSDVTGSDAIWSDLSDMGVDWELHNGTGILLFLLPEETDLEQLNLCIFAGTESEDAHRYAQNAVAVQTVYEIPLTVTDSSDMEGMDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>R5UWV7</accession>
    <name>R5UWV7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator LacI family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_00613</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000008">
        <property type="protein sequence ID" value="CCZ82808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00613"/>
    <dbReference type="EnsemblBacteria" id="CCZ82808">
        <property type="protein sequence ID" value="CCZ82808"/>
        <property type="gene ID" value="BN566_00613"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01392">
        <property type="entry name" value="HTH_LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000843">
        <property type="entry name" value="HTH_LacI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046335">
        <property type="entry name" value="LacI/GalR-like_sensor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00356">
        <property type="entry name" value="LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13377">
        <property type="entry name" value="Peripla_BP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00354">
        <property type="entry name" value="HTH_LACI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50932">
        <property type="entry name" value="HTH_LACI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH lacI-type" evidence="4">
        <location>
            <begin position="5"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ82808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="349" mass="38904" checksum="77CA0F752B4895A3" modified="2013-07-24" version="1">MAKAVRMSDIAEQLQISTVTVSKALAGKDGVSEELRKKIQKLAVEMGYQFQKNKQEQTPCYTIGIITSYRYIEKGQSFYWAMYERVLFHLSQGNHIGILEVISESAEQTCTMPRLIAENRIQGCIVIGKFSLSYYKMLEQASVPCLVLDAFQAELQQDSVISDGYYGMYLMTKHLLQAGHREIAFVGSIEETSSILDRYYGYCRAMREAGILVTEKQVLPDRDAEGKIAISLEKLSKMPTAFACNCDSTAYILISLLQKAGFSIPNDISVVGFDDFIFAELANPPITTYAVDMNLMSKKGVRQLLARIKNPAIPIRHIVVSGTMIHRKSVRNLPLAEPASLTSKEGNPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5UWX1</accession>
    <name>R5UWX1_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CotJB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00623</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000011">
        <property type="protein sequence ID" value="CCZ82818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00623"/>
    <dbReference type="EnsemblBacteria" id="CCZ82818">
        <property type="protein sequence ID" value="CCZ82818"/>
        <property type="gene ID" value="BN566_00623"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024207">
        <property type="entry name" value="CotJB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016571">
        <property type="entry name" value="Spore_coat_assembly_CotJB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12652">
        <property type="entry name" value="CotJB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010606">
        <property type="entry name" value="Spore_coat_CotJB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CotJB" evidence="1">
        <location>
            <begin position="7"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="89" mass="10909" checksum="E5D8F1E5D10F7CC7" modified="2013-07-24" version="1">MREQELLLRSLQQCDFTLYELQLYLDTHPNCKRAMEQYRKHLEMKQKAEKMYVEKYGPIRPSQSNTTERWNWVDAPWPWEKEANCDVDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5UX13</accession>
    <name>R5UX13_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00658</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000012">
        <property type="protein sequence ID" value="CCZ82853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00658"/>
    <dbReference type="EnsemblBacteria" id="CCZ82853">
        <property type="protein sequence ID" value="CCZ82853"/>
        <property type="gene ID" value="BN566_00658"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="263" mass="28652" checksum="6343DD4DDEA5069B" modified="2013-07-24" version="1">MWNHSEPEDSFGEPPKSHTKGKGIAFALVPTILLLTGIGFRIARTAQKAERLISHHTSTTVTAATVTTPTKPARAAAKQVKTEPATAAKTAAQDVRNGIYTSGDYEVGVDLPAGTYLAVYDGPSGDQKFVMQISSSSDPNDSDAVLTSQTFHPNQVYFQVIDGQFLHISWANFYDTEQVSITLEPFAHDGMYQVGKDIPAGTYQLELDKGEYVYPETAGYQIYSQMASPQPLPKSKGSYTDSITLQDGEYVQLDDCHFQQNAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXB9</accession>
    <name>R5UXB9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Serine hydroxymethyltransferase</fullName>
            <shortName evidence="7">SHMT</shortName>
            <shortName evidence="7">Serine methylase</shortName>
            <ecNumber evidence="7">2.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">glyA</name>
        <name evidence="10" type="ORF">BN566_00748</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine</text>
            <dbReference type="Rhea" id="RHEA:15481"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="EC" id="2.1.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7 8">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Amino-acid biosynthesis; glycine biosynthesis; glycine from L-serine: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the SHMT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.1" evidence="7"/>
    <dbReference type="EMBL" id="CBAR010000030">
        <property type="protein sequence ID" value="CCZ82948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00748"/>
    <dbReference type="EnsemblBacteria" id="CCZ82948">
        <property type="protein sequence ID" value="CCZ82948"/>
        <property type="gene ID" value="BN566_00748"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="UniPathway" id="UPA00288">
        <property type="reaction ID" value="UER01023"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004372">
        <property type="term" value="F:glycine hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019264">
        <property type="term" value="P:glycine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00378">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00051">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001085">
        <property type="entry name" value="Ser_HO-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019798">
        <property type="entry name" value="Ser_HO-MeTrfase_PLP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039429">
        <property type="entry name" value="SHMT-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680">
        <property type="entry name" value="PTHR11680"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00464">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000412">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00096">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4 7" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="6 7 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SHMT" evidence="9">
        <location>
            <begin position="14"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="129"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="365"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="7 8">
        <location>
            <position position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000412-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ82948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="416" mass="45145" checksum="35FE6675A892558C" modified="2013-07-24" version="1">MYQDMMDTIGFVGKYDPEVSAAMEKELARQRRNLELIASENIVSPAVMAAMGSVLTNKYAEGLPHKRYYGGCEYVDVVEEIAIDRAKKLFGAKFANVQPHSGAQANTAVYFALLQPGDTVLGMSLAHGGHLTHGSPVNISGKYFNFVSYGLGDDETIDYDKVAALAKEHKPKMIVAGASAYPRIIDFPKLAEIAKSVGAYLMVDMAHIAGLVAAGVHPSPVPYADITTTTTHKTLRGPRGGLILCNDEELAKKINKAVFPGTQGGPLMHVIAGKAVCFGEAMKPEFKEYGKHIVENAQALAKGLVQRGFNLVSGGTDNHLMLVDLRPFSITGKELERRLDEVYITVNKNAIPNDPEKPFVTSGIRVGTPAVTTRGLGVAEMDQIAECMYLTATQFDKADEIREMVTAICKQYPLYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXD7</accession>
    <name>R5UXD7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Peptidase U32</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00763</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000032">
        <property type="protein sequence ID" value="CCZ82963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00763"/>
    <dbReference type="EnsemblBacteria" id="CCZ82963">
        <property type="protein sequence ID" value="CCZ82963"/>
        <property type="gene ID" value="BN566_00763"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071704">
        <property type="term" value="P:organic substance metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001539">
        <property type="entry name" value="Peptidase_U32"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01136">
        <property type="entry name" value="Peptidase_U32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ82963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="338" mass="38979" checksum="F154ECEE3797BD57" modified="2013-07-24" version="1">MQLLLPIRSIEQATFYLEDGIREFYCGYISKEWIATFNKQHEQRWTTLQVSMNRRDYLTSNLTDLQELKEIANLCNAYQATLFVTLNAAFYAPIAYPYLEKWLQELSQIGIQHLIVSDIGMMQLIAKHYPNFKITVSCENQVLNADAVTFYRQFQPERIVFPRHITISEITPILAENPDIEFECFLLSSRCVFDDGNCRCIHDIEPICNEAWITKFFRTDGKNVSFSENRALKNANQDMTDWIYGIEQSTNQGFGCGKVLCSICSVYNLSQYPNFTSLKVVGRGMRFPKQALDGMKYVIELSKSHATLSDYQEFAQKFFCCDTLCSSMASCNMRGAQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXP4</accession>
    <name>R5UXP4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Dockerin type 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00650</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000012">
        <property type="protein sequence ID" value="CCZ82845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82845">
        <property type="protein sequence ID" value="CCZ82845"/>
        <property type="gene ID" value="BN566_00650"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002105">
        <property type="entry name" value="Dockerin_1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00404">
        <property type="entry name" value="Dockerin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004406531" evidence="1">
        <location>
            <begin position="27"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="2">
        <location>
            <begin position="37"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ82845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="465" mass="51548" checksum="0BFCEB2EC522D1DE" modified="2013-07-24" version="1">MNKKRIAALVLSAAMATSGLGISAQAKTLSEQVKTPKTVLVGDCNQDGTVDLSDLVSMQLYLLRQKTVGEMPEEQSAWDLNLDAQVNIVDLCLLKQILLQQKQVTQQYRTDILSTSSAGVVPPEVEIHSVEELTAYLTPYTTESERETFCERYDADFFAESVLLLKPMGKTMKAYACQVTGADAKTLQVSYGCIWEKEKPTGIQQFAWMQVAIPKMQDHEQVVWQSVTPEVTLNWCAVTGNDAFSDGSETRIDSTEELQTYLETILQPDEIPEYLQQYDDAFFAESTLVVHSFQLRNAAPLEYTAIQKNQALAILLSYQQAGGKQLTNYLAFAAVRRAELSEISNVGFLAFGGTSEDDADWLYYDAPDQKHSLVVLQNSDLFSGTVSVYRCDADGVEKTLIGSGGTDDGYQPFTKYGTWSEDADGNAVYSNGVSYWITWKDAEAVVTYHTISDDNNTCDTVVVPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXQ6</accession>
    <name>R5UXQ6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATP-dependent Clp protease proteolytic subunit ClpP</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00876</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000052">
        <property type="protein sequence ID" value="CCZ83088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00876"/>
    <dbReference type="EnsemblBacteria" id="CCZ83088">
        <property type="protein sequence ID" value="CCZ83088"/>
        <property type="gene ID" value="BN566_00876"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023562">
        <property type="entry name" value="ClpP/TepA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00574">
        <property type="entry name" value="CLP_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="248"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="276" mass="29575" checksum="71C7387270940C6E" modified="2013-07-24" version="1">MDMQTSWTASTSAETPSATDESEQQLQEAVLIAESGSIRPQNAKHLIHCLTIIGQVEGHYILPAENKTTKYEHIIPALIAIEQDRSIEGLMILLNTVGGDVEAGLAIAEMIAGMQTPTVSLVLGGGHSIGVPLAVSAKRSFIVPSATMTIHPVRTNGLVVGVPQTFAYFNQIQNRIIEFITRCSHIQTERLLSFMHRTDALATDIGSILTGEEAVAEGLIDQLGSLQDAISCLYELIEAAPLRYTDASVPSSTLGKDGCSGTNGKIKTKEKSHPQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>R5UY49</accession>
    <name>R5UY49_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator LacI family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_01034</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000082">
        <property type="protein sequence ID" value="CCZ83253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01034"/>
    <dbReference type="EnsemblBacteria" id="CCZ83253">
        <property type="protein sequence ID" value="CCZ83253"/>
        <property type="gene ID" value="BN566_01034"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01392">
        <property type="entry name" value="HTH_LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000843">
        <property type="entry name" value="HTH_LacI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046335">
        <property type="entry name" value="LacI/GalR-like_sensor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00356">
        <property type="entry name" value="LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13377">
        <property type="entry name" value="Peripla_BP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00354">
        <property type="entry name" value="HTH_LACI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50932">
        <property type="entry name" value="HTH_LACI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH lacI-type" evidence="4">
        <location>
            <begin position="5"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="340" mass="38312" checksum="4B9466B81D1AEDBB" modified="2013-07-24" version="1">MSKLVRMSDIAQRLHISTVTVSKALSGKDGVSDALRLKIQQTAEQMGYQRKTIQAERSNRFTVAVLIERYHVNRKNSFSWTIYEGLLTALEIRGHTGKLEDVPASDVECGILPESIQQHQVDGIILLCPFPMEYCKKLQETGIPIVVGDVMDANFPQDTVLSDSYYGMYTLTNYILQKGHRDIMFVGAIHKNPVLADRFYGFCRAMMEAGIPVTQNHILVDRDENGHYALSASQLSHIPTAFICSRDSSAYQLLKILKEKNIRVPEDVSITGFDDYILSSLCTPKITTYSIDVAKLTDSCIWLLLDRIAQPDRPLRHIVISGKIMERDSVRTLSTNPESR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5UY67</accession>
    <name>R5UY67_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00788</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000036">
        <property type="protein sequence ID" value="CCZ82995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82995">
        <property type="protein sequence ID" value="CCZ82995"/>
        <property type="gene ID" value="BN566_00788"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="468" mass="51548" checksum="710E138882D310D4" modified="2013-07-24" version="1">MSKNVPSNHEPPISPADWDAIVAAAFSPDAEPHIFSDRYQKQKQKQEEFLMPKKSIRGRKTIVIAAILAAAVVAIPTSAYAINRIYQASIQSTGSYQKDVVIDAGDSTLDTRTQSLQVGWMPDGMTLDKSSGKYHDDQNRGVTMLFWKMQDGDFLQTVNRGIVHSETKTINGNQVFFLQRDSANLPDGQMDYRNIVWVAFSNTPYAAQFYATDDLTKEELTQIAEHLSLSPAEQETASLWQPEESLIESSGDAPATACDVNQLSYVNIGDTLTTEETVSLKLDALSTQNTFAGISTDSIGQDCDYSRYQNADGTIADNTRTWYRSGDGVNTLDEAIKTETIPQTILTFQLTYTNNGTEAVETCVCPQLFRMQADGTFPAYTAEAEKEYSVDSCDLNITGEFFSFSSSGSHSKNNVLLQPGETQTVTVAFLVDADQLDELYLVPAPHGYELSEEVTQSPILQVASALNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYH2</accession>
    <name>R5UYH2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01144</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000093">
        <property type="protein sequence ID" value="CCZ83368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01144"/>
    <dbReference type="EnsemblBacteria" id="CCZ83368">
        <property type="protein sequence ID" value="CCZ83368"/>
        <property type="gene ID" value="BN566_01144"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002145">
        <property type="entry name" value="CopG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008687">
        <property type="entry name" value="MobC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05713">
        <property type="entry name" value="MobC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01402">
        <property type="entry name" value="RHH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RHH_1" evidence="1">
        <location>
            <begin position="66"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="MobC" evidence="2">
        <location>
            <begin position="117"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="168" mass="19931" checksum="68F740B856535EB7" modified="2013-07-24" version="1">MQSQRLKLKSRTEGVIQTQQARYLRITISQKFNVPIQCLQEGHPLNPEQKEMRKIAEIEPHNRYMQIRLTEAEYEAIERKFRNSGLRSRSEFIRAMIFEGYIVNFDEEKFDKIYRLVGSISNNINQIAVRANSTNKVYTEDIVNIKEGQDKIWQLLNSLQSKLLRLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYK5</accession>
    <name>R5UYK5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Translation factor SUA5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01174</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000094">
        <property type="protein sequence ID" value="CCZ83398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01174"/>
    <dbReference type="EnsemblBacteria" id="CCZ83398">
        <property type="protein sequence ID" value="CCZ83398"/>
        <property type="gene ID" value="BN566_01174"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038385">
        <property type="entry name" value="Sua5/YwlC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005145">
        <property type="entry name" value="Sua5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006070">
        <property type="entry name" value="YrdC-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03481">
        <property type="entry name" value="SUA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01300">
        <property type="entry name" value="Sua5_yciO_yrdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="SSF55821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51163">
        <property type="entry name" value="YRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YrdC-like" evidence="1">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="242" mass="26095" checksum="331EE0D497ED9B1C" modified="2013-07-24" version="1">MILPKQDCIPSVTSGGLDTVGIRMPSHPIARRLITLSKTAIAAPSGNRSGYPSPTTAAHMMHDLNGKIAAVIDGGACQVGVESTVICFEDAQTVRILRPGYITKEDLEQVAKTAILDPAILHDIQAGQTVRSPGMKYQHYSPQANVILVEGSDAAFCAYVSQHQTADCYALVFDGEEQNLSIPCLTYGKNSAEQAQHLFDCLRHCDEIGAKTVYVRAPKRDGIGLAVYNRLIRAAGFEVIQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZ27</accession>
    <name>R5UZ27_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cellulase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN566_01326</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000130">
        <property type="protein sequence ID" value="CCZ83558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01326"/>
    <dbReference type="EnsemblBacteria" id="CCZ83558">
        <property type="protein sequence ID" value="CCZ83558"/>
        <property type="gene ID" value="BN566_01326"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040877">
        <property type="entry name" value="CBM65_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018087">
        <property type="entry name" value="Glyco_hydro_5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18259">
        <property type="entry name" value="CBM65_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00659">
        <property type="entry name" value="GLYCOSYL_HYDROL_F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="1" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="3 5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004394651" evidence="7">
        <location>
            <begin position="33"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="8">
        <location>
            <begin position="543"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="527"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ83558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="608" mass="68047" checksum="D7A79CD60A78DFDD" modified="2013-07-24" version="1">MKHQKMNRILAAALSAACLVPFAAMVPVDADAADVMSALEITQEMGLGFNIGNSLDSTGYGNYDDITSFEKSYGNPAVTKEMVDTIKAKGFDSVRIPTSWFRHVTKTTDENGNPVYTIDSRWLERVKEVVDYAYQQGMYVILNLHHEEWINRSDFATAYDEMAPQLKQMWTQIATYFADYDQHLIFEGMNEPRAANSGALEWNGNEACYEVVNKLDNDFIETVRSVDSPYKDTRLLMIPGYAASAYSSIYGYLEIPEDDNYIAASVHAYCPYNFAMGDGDHMTFSDANKTELDKIFKDIQTTFTNEDIPVVIGEFSASNYNNTEARVDWATYYLNWAKKLGIPCFLWDNNAINNGSNASESHGYLDRSSLQWFSASEPVIDAMLSVLDDSSVKWGSERHLPEYKHSDLSDATSVIYENAEGGTFAADSNYFDAQMVSGSELSEDVEIAVQYTDIMNPDLNFMDAEWGNWTTVAAYDVDKEKGIAYFSYADIKKAWGDLSTLNSVCVGTGNSSSRIYKIALLPAAKLVEPTDPTDPTDPTDPSETTIKGDINGDGALNVVDLIGMKRKLFILTDAPGLAWPLEKYDWNEDLKFDLLDVVDFTKFLIRKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZ96</accession>
    <name>R5UZ96_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01394</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000147">
        <property type="protein sequence ID" value="CCZ83658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83658">
        <property type="protein sequence ID" value="CCZ83658"/>
        <property type="gene ID" value="BN566_01394"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="85" mass="9889" checksum="7B843A1F981B5C89" modified="2013-07-24" version="1">MFESGVKKYVFVRVNYEFGFPVTFHDVAHIECAYCQMYDKYKNRCNITGEYIVEPTRYVGFECPLELAEGGSEEHGRTEDGTETR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZE9</accession>
    <name>R5UZE9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01454</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000155">
        <property type="protein sequence ID" value="CCZ83718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01454"/>
    <dbReference type="EnsemblBacteria" id="CCZ83718">
        <property type="protein sequence ID" value="CCZ83718"/>
        <property type="gene ID" value="BN566_01454"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="150" mass="17612" checksum="BA58ADC2F93897FF" modified="2013-07-24" version="1">MKIDEILDLMDQLLDHAKPIPFASHKVIVDGDRLRELINDMRMNKPEEIRRAKLIEFDCDRIIQEAQTNGERIVQEAEQRAKKLVEQQTITMEARERAVEMLTKAQAASEEIKNASESYVMHLLTETEQYLNTNLQEVQQTKNRIHNRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZI5</accession>
    <name>R5UZI5_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable nicotinate-nucleotide adenylyltransferase</fullName>
            <ecNumber evidence="6">2.7.7.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Deamido-NAD(+) diphosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Deamido-NAD(+) pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Nicotinate mononucleotide adenylyltransferase</fullName>
            <shortName evidence="6">NaMN adenylyltransferase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">nadD</name>
        <name evidence="8" type="ORF">BN566_01498</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 6">Catalyzes the reversible adenylation of nicotinate mononucleotide (NaMN) to nicotinic acid adenine dinucleotide (NaAD).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>ATP + H(+) + nicotinate beta-D-ribonucleotide = deamido-NAD(+) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22860"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <dbReference type="EC" id="2.7.7.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 6">Cofactor biosynthesis; NAD(+) biosynthesis; deamido-NAD(+) from nicotinate D-ribonucleotide: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the NadD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.18" evidence="6"/>
    <dbReference type="EMBL" id="CBAR010000161">
        <property type="protein sequence ID" value="CCZ83763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01498"/>
    <dbReference type="EnsemblBacteria" id="CCZ83763">
        <property type="protein sequence ID" value="CCZ83763"/>
        <property type="gene ID" value="BN566_01498"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00332"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004515">
        <property type="term" value="F:nicotinate-nucleotide adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02165">
        <property type="entry name" value="NMNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00244">
        <property type="entry name" value="NaMN_adenylyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005248">
        <property type="entry name" value="NadD/NMNAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00482">
        <property type="entry name" value="TIGR00482"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 6" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="4 6" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CTP_transf_like" evidence="7">
        <location>
            <begin position="6"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="221" mass="25574" checksum="8CD88E097B001B54" modified="2013-07-24" version="1">MRRIGIFGGSFNPIHNGHLHLAKTAMEQFSLDHVLFMPTYISPFKQNAADVADSIHRLAMCRLAIAPYPCFSVSDYELQQKTVSYTIHTISALRQEWTDVELVLLMGSDMLLQFQNWYRWQELLTMTELGVIARETDDRVVLEQAAQELSAYGTIQLCCDRVLPISSTKIREMLKKNQDCTCYLPENVVKYIVFHQLYQRTEEKENGKRKQGNFDGKRICD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZI7</accession>
    <name>R5UZI7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Carboxynorspermidine/carboxyspermidine decarboxylase</fullName>
            <ecNumber evidence="5">4.1.1.96</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN566_01204</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>carboxynorspermidine + H(+) = CO2 + norspermidine</text>
            <dbReference type="Rhea" id="RHEA:34099"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57920"/>
            <dbReference type="ChEBI" id="CHEBI:65070"/>
            <dbReference type="EC" id="4.1.1.96"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>carboxyspermidine + H(+) = CO2 + spermidine</text>
            <dbReference type="Rhea" id="RHEA:34095"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
            <dbReference type="ChEBI" id="CHEBI:65072"/>
            <dbReference type="EC" id="4.1.1.96"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the Orn/Lys/Arg decarboxylase class-II family. NspC subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.96" evidence="5"/>
    <dbReference type="EMBL" id="CBAR010000102">
        <property type="protein sequence ID" value="CCZ83430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01204"/>
    <dbReference type="EnsemblBacteria" id="CCZ83430">
        <property type="protein sequence ID" value="CCZ83430"/>
        <property type="gene ID" value="BN566_01204"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045312">
        <property type="term" value="P:nor-spermidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06829">
        <property type="entry name" value="PLPDE_III_CANSDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009006">
        <property type="entry name" value="Ala_racemase/Decarboxylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022643">
        <property type="entry name" value="De-COase2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005730">
        <property type="entry name" value="Nsp_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43727:SF1">
        <property type="entry name" value="PTHR43727:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00278">
        <property type="entry name" value="Orn_DAP_Arg_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038941">
        <property type="entry name" value="NspC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50621">
        <property type="entry name" value="SSF50621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="SSF51419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01047">
        <property type="entry name" value="nspC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Orn_DAP_Arg_deC" evidence="10">
        <location>
            <begin position="15"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR038941-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ83430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="380" mass="42538" checksum="A6AA3A8496BFEDA9" modified="2013-07-24" version="1">MHLPFSTDSLQTPCYVTDVAALKRNGEILKSVQQETGCKILLAQKAFSMFATYPILKPYLAGTTASGLYEAKLGREEMGGEVHIFSPAYPEQDFPEICAISDHIVFNSFHQWQLHKAFVQSCGRPISCGIRINPEYAEVETDLYNPCIPGSRMGTTISQFSEEAFDGLDGIHFHTMCEQDAPVLERTLEHVMEKFDPYLKRCKWVNFGGGHHITRPGYDLPCLIRCIRKIQERYGVQVYLEPGEAVALNAGYLVATVLDFVQNGMEIALLDASAACHMPDVLEMPYRPEIIGAGKPQEKKYTYRLAGNTCLAGDVIGDYSFDEPLQAGDKLIFCDMAIYSMVKNNTFNGMPLPDLVLRQEDGSFTLVKRFSYSDFKGRLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZU5</accession>
    <name>R5UZU5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01269</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000118">
        <property type="protein sequence ID" value="CCZ83500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83500">
        <property type="protein sequence ID" value="CCZ83500"/>
        <property type="gene ID" value="BN566_01269"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="159" mass="17500" checksum="1465E1F649D91438" modified="2013-07-24" version="1">MREKHIMGGLLCSCLLLGSLTGCGGDWKGKLLYGYEKTRSSLGGFQRTSKQQLYGMRLDGEDAYTGTYQADCRNVRGKDVVFGGCSIQTRTLWLTGTVGARNGIVEIYVLNGDKEKEYLTPNEQGMFSKIIQNQGGNCYVVVDYADFTGTVTLTSEYPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZY5</accession>
    <name>R5UZY5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted SAM-dependent methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01642</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000172">
        <property type="protein sequence ID" value="CCZ83913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01642"/>
    <dbReference type="EnsemblBacteria" id="CCZ83913">
        <property type="protein sequence ID" value="CCZ83913"/>
        <property type="gene ID" value="BN566_01642"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016429">
        <property type="term" value="F:tRNA (adenine-N1-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006901">
        <property type="entry name" value="TrmK"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018637">
        <property type="entry name" value="TrmK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="224" mass="25005" checksum="23980C41BF33324E" modified="2013-07-24" version="1">MLNQRLQACAAWIADGKTVCDVGTDHAYLAAELLRKERCQHVIASDIGEGPLQTARRTLEQAGLLQRATLLLSDGLEQVDGTDVSHVVIAGMGGETIIHILQPCDWVKQGVQLILQPMTKVPVLRQWLAKNGYAILQEQVVKDGKFFYNLLRVQYDGVVRTLTPLEREIGVQDWSNAVVQAYGRRKRERLQTIAEQIQNSNPAMAADDLQIVAELDRRLQPESV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZZ7</accession>
    <name>R5UZZ7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01652</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000173">
        <property type="protein sequence ID" value="CCZ83923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01652"/>
    <dbReference type="EnsemblBacteria" id="CCZ83923">
        <property type="protein sequence ID" value="CCZ83923"/>
        <property type="gene ID" value="BN566_01652"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="119" mass="13934" checksum="9A6CF2AF4EFA6E19" modified="2013-07-24" version="1">MYKAFEKWLNGVLEENMPIPGVALNFNLYEEADFCWSIQLIGASCFDEEEEDWCCEETFCSGENRFSWKEHAEWEEILNVSCKMIRQYISNGKYAEALKKYEAIAAGFVDGDLEILYQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>R5V013</accession>
    <name>R5V013_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">DivIVA domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_01338</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000131">
        <property type="protein sequence ID" value="CCZ83570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01338"/>
    <dbReference type="EnsemblBacteria" id="CCZ83570">
        <property type="protein sequence ID" value="CCZ83570"/>
        <property type="gene ID" value="BN566_01338"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019933">
        <property type="entry name" value="DivIVA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007793">
        <property type="entry name" value="DivIVA_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35794">
        <property type="entry name" value="PTHR35794"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05103">
        <property type="entry name" value="DivIVA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03544">
        <property type="entry name" value="DivI1A_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="4 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="162"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="21"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="114"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="164"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="223" mass="25098" checksum="FB6AFCF0B6FAAA09" modified="2013-07-24" version="1">MISAKEIHELRFEKAAFGYKQEDIDEFLTQLEQEVEIAEQEMADSNEKIQVLADKVREYMRDEDALKDALLGAQREGHRIIAEANAKADGIIADAKKRADAMMDEVTVKHEALLAKNQAEIDEAHQALADARKQVADFKKALFDMYKEHLQMLSTMPEEDDLEFGFTPQQDEPEEEVPEQEIPVVSAPTSDPFASSQFSAKTIQGSYDSHFGDLSDADEDEDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>R5V030</accession>
    <name>R5V030_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Probable cell division protein WhiA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">whiA</name>
        <name evidence="7" type="ORF">BN566_01358</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in cell division and chromosome segregation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the WhiA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000144">
        <property type="protein sequence ID" value="CCZ83595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01358"/>
    <dbReference type="EnsemblBacteria" id="CCZ83595">
        <property type="protein sequence ID" value="CCZ83595"/>
        <property type="gene ID" value="BN566_01358"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043937">
        <property type="term" value="P:regulation of sporulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01420">
        <property type="entry name" value="HTH_type_WhiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027434">
        <property type="entry name" value="Homing_endonucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003802">
        <property type="entry name" value="Sporulation_regulator_WhiA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023054">
        <property type="entry name" value="Sporulation_regulator_WhiA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039518">
        <property type="entry name" value="WhiA_LAGLIDADG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37307">
        <property type="entry name" value="PTHR37307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02650">
        <property type="entry name" value="HTH_WhiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14527">
        <property type="entry name" value="LAGLIDADG_WhiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55608">
        <property type="entry name" value="SSF55608"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00647">
        <property type="entry name" value="DNA_bind_WhiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1 4" id="KW-0132">Cell division</keyword>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LAGLIDADG_WhiA" evidence="6">
        <location>
            <begin position="116"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="HTH_WhiA" evidence="5">
        <location>
            <begin position="210"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF14527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ83595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="304" mass="34514" checksum="69F7A0CEE71907BA" modified="2013-07-24" version="1">MSFSQDVRQALKRTILDKDQAFSCLYGILLYSRVFTSEQICLQSESETIVDLLPQLLHTVFSFPKGTVTVERGRGGLYSLSLQNPAAVAEICERYHIHLDNRQINLCNIVQNSMPAFLAGVFLTCGNLMDPNKSYHLEFVTPTSVLCNDLSLFLQQLLGIRGHVFQRKQSFVLYVKDSEQIEDMLTFMGAQQCTLDLMNIKIKKDMRNKANRMRNCDAANIDKVVSAAMRQTEEILLLAEKRGLDTLPPDLQELAELRLENPELSLKELGELLHPPIGRSGVNHRFQKLSMMAKELREHVGTEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5V045</accession>
    <name>R5V045_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00142</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000146">
        <property type="protein sequence ID" value="CCZ83615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83615">
        <property type="protein sequence ID" value="CCZ83615"/>
        <property type="gene ID" value="BN566_00142"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008965">
        <property type="entry name" value="CBM2/CBM3_carb-bd_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49384">
        <property type="entry name" value="SSF49384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004394858" evidence="3">
        <location>
            <begin position="33"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="285"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="309" mass="31474" checksum="4BE88209E43B7F78" modified="2013-07-24" version="1">MKSTFKKALAAVSAAAVVATSAAVFTAVPAQAVNTPSIGQVTVTLDELQANNYLVTVPVSIYGNTDGWDSIAFGLQWSTDELTYSKCVSGGSIANAMFTDGIQVSGSGFQLNPAGIGGWITYATTPTGDGPTYVTGDDPVINITFKVTENAQPGDIYYIDGLERSYDGAYVPKVTHGSEVVTGAPSQGYIQIEGVQTTTTEATTTTTTTTTTTTTTTTTTTTTTTAKPTTTTTTTTTTAKPTTTTTTTTSAAATTTTKAATTTKAAQQTTKKNTGSTSSPKTGSMIPVAGAVAAIAVIGGVALVSKKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5V076</accession>
    <name>R5V076_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01391</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000147">
        <property type="protein sequence ID" value="CCZ83655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83655">
        <property type="protein sequence ID" value="CCZ83655"/>
        <property type="gene ID" value="BN566_01391"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="40"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="110" mass="12895" checksum="B19204C5F779F658" modified="2013-07-24" version="1">MVKHCDMCGIEIADTDYDTLGRYNAVKYCQECAAIRKKIANKNYRQKRKMEKQVAKATEEHELTEAAKACRKVRRAANEQTRLLKEENKFLREELMKERAKKDPRTGSNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0H3</accession>
    <name>R5V0H3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HAD superfamily (Subfamily IIIA) phosphatase TIGR01668</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01505</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000161">
        <property type="protein sequence ID" value="CCZ83770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01505"/>
    <dbReference type="EnsemblBacteria" id="CCZ83770">
        <property type="protein sequence ID" value="CCZ83770"/>
        <property type="gene ID" value="BN566_01505"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008962">
        <property type="term" value="F:phosphatidylglycerophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006549">
        <property type="entry name" value="HAD-SF_hydro_IIIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010021">
        <property type="entry name" value="PGPP1/Gep4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288:SF25">
        <property type="entry name" value="PTHR19288:SF25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01662">
        <property type="entry name" value="HAD-SF-IIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01668">
        <property type="entry name" value="YqeG_hyp_ppase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="168" mass="19120" checksum="AFE138E54D2EE3BF" modified="2013-07-24" version="1">MFRITAALRSVCDITPTMLQHFGIRGLLLDLDNTLTTHDNPRPAEGVLDWIAVMKENGIAMCIVSNNHPPRVKPFADLLGLPFVCEGKKPLSKGFREARTVIGLPWKELAVVGDQIFTDVLGANLKRLKCIFVFPIAHEQTRFFRFKRRMEVPFLPREIYQEEKGTQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0N2</accession>
    <name>R5V0N2_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">[Ribosomal protein S18]-alanine N-acetyltransferase</fullName>
            <ecNumber evidence="1">2.3.1.266</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01560</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Acetylates the N-terminal alanine of ribosomal protein S18.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetyl-CoA + N-terminal L-alanyl-[ribosomal protein bS18] = CoA + H(+) + N-terminal N(alpha)-acetyl-L-alanyl-[ribosomal protein bS18]</text>
            <dbReference type="Rhea" id="RHEA:43756"/>
            <dbReference type="Rhea" id="RHEA-COMP:10676"/>
            <dbReference type="Rhea" id="RHEA-COMP:10677"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64718"/>
            <dbReference type="ChEBI" id="CHEBI:83683"/>
            <dbReference type="EC" id="2.3.1.266"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the acetyltransferase family. RimI subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.266" evidence="1"/>
    <dbReference type="EMBL" id="CBAR010000165">
        <property type="protein sequence ID" value="CCZ83825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01560"/>
    <dbReference type="EnsemblBacteria" id="CCZ83825">
        <property type="protein sequence ID" value="CCZ83825"/>
        <property type="gene ID" value="BN566_01560"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006474">
        <property type="term" value="P:N-terminal protein amino acid acetylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006464">
        <property type="entry name" value="AcTrfase_RimI/Ard1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13673">
        <property type="entry name" value="Acetyltransf_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01575">
        <property type="entry name" value="rimI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="2">
        <location>
            <begin position="5"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ83825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="146" mass="16302" checksum="2AD4DF86F4D24F80" modified="2013-07-24" version="1">MMQKVKILPFSQEQLPAAAALAQRCFPDPWSLALFEAAFSQPNTTLFAAMAGDTLAGYLILQRLGDEESVDDIAVAPEFRRQGIAQQLLETAHAQFPTCPFLLEVREHNTAALALYQKLGYQQVGFRKRYYTNPNEGAVLMTRPPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0T6</accession>
    <name>R5V0T6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_01938</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84213">
        <property type="protein sequence ID" value="CCZ84213"/>
        <property type="gene ID" value="BN566_01938"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005451">
        <property type="term" value="F:monovalent cation:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1450">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030151">
        <property type="entry name" value="NhaP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006037">
        <property type="entry name" value="RCK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036721">
        <property type="entry name" value="RCK_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32507:SF7">
        <property type="entry name" value="PTHR32507:SF7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02080">
        <property type="entry name" value="TrkA_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116726">
        <property type="entry name" value="SSF116726"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51202">
        <property type="entry name" value="RCK_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="87"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="118"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="186"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="220"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="266"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="291"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="330"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="364"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="RCK C-terminal" evidence="5">
        <location>
            <begin position="401"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="domain" description="RCK C-terminal" evidence="5">
        <location>
            <begin position="467"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ84213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="538" mass="57776" checksum="1E96FB4447B58E5C" modified="2013-07-24" version="1">MIQLLLLLSVSTIACILCNKITAKIGIPMLLAFLCVGMLCGEDGILGISFNDYQLTENVCTAALILIIFYGGFGTKWSAAKPVAGRAILLSTVGVFLTAAAVGGFCHIVLHVGLLESLLIGSVISSTDAASVFSILRAKKLNLKYGTASLLEVESGSNDPIAYLMVTIVVAMMETSVSAGSVLYQVFAQIVFGVGIGAVLALVVLFFLRRFQFDTSGFDLVFMLAVAILSYVVPTMIGGNGYLSAYIAGIILGNAELSNKKNLVHFFDGVTGLMQLLVFFILGMLCTPTKLIGVLLPALGISIFLTFIARPLVVGVLLTPFRAKFSQQLLISWAGLRGATSAIFAITAVASMEAAGSVTLKYDLFHLVFCIVLFSIALQGTFLPWVSKKLHMIDDSQNVLKTFTDYTDEEIIQTLQLPIHANHAWIDQPVSSIALPPDTLLAMLIRNGKTVIPKGNTKIEQGDVVVLSAGNCQQTANMQLSELVIDDHHKWNGKQIRNLNLGKKTLILMIQRGSETIIPQGDTEILAGDTVVLHSETT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0U7</accession>
    <name>R5V0U7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01948</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01948"/>
    <dbReference type="EnsemblBacteria" id="CCZ84223">
        <property type="protein sequence ID" value="CCZ84223"/>
        <property type="gene ID" value="BN566_01948"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018580">
        <property type="entry name" value="Uncharacterised_YfhO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38454">
        <property type="entry name" value="PTHR38454"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09586">
        <property type="entry name" value="YfhO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="236"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="298"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="328"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="360"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="386"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="411"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="837"/>
            <end position="856"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="878" mass="99177" checksum="8D653C7C0F3E1CC3" modified="2013-07-24" version="1">MRSGLLASNRNNPKRYLLLSMLLPMLLVAVGWIVLEVHPFGTRQILVTDFWHQYYPFLCILQEKLKHGESLLYTWQSGMGSNFAAMMAYYTASPLNLLTLLVPASMLRDTVTGLVLLKIGAAGGFFAAFLKGTYGRNDSSLCLFGTMYALCDFMMGYSWNIIWLDTVALLPLVMLGVVRLVRTGSYHLYTVSLALAMLANFYIGFFICIFTVIAYFCICIFTGMGFKRFLTRSGKMLAATILGLAISSILLLPTYYALQNTYSIHNTFPQHSTFYETWSDLLSQLLAYQIPTVKEGKPNLYCGVLCVVLVGMFLRARSIRIREKVGAILVLAFLLVSCNWNILNYLWHGCHFPNMLPYRFSFLFSFALLTLAYRAYLLLLERRMKWLDLIAMVLCSGVFLFLVYTTDRKQVVLYTGMTMLAYLLWMLLHKLGLLHRRVFAAGMLVVMLVEMGCHVRIATEAVSTSDYTSYPEKYESVEALLSQIEEQEPENAFYRIELTSWYTLNDPALYQYRGVSQFSSLANCKVSTFLKKLGMPASEAGNRYYYAHSTPYLDMLLGVRYLIQKTGFMPEAETVEKVAVSDTVTSYRNRYALPVGFMVNEETADFSIDTSENAFEVQNDLFAKMTGIKEPLYTAVDVKDVGHNGVEVSRNGYGYYTYFPMSTAAEPYLKYNFMPEQDTLLYAYLSVDKVEEVDIYQNERYTFSQKLAKQPYIFPLGSYKAGEKATLKWNVDRKSIQNGAITLFVYALNQDVLEQGYQQLATGGLQVTDWSDTRLTGTLNADHDGICYLSIPAEDGWHVTVDGKQTEPISIGGAMLGVPVSSGTHTIALRYCPKGTVVGTICCGSAILLLTFCFVIEQKQKRACRSNSVGRRRKTCEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5V114</accession>
    <name>R5V114_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00027</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000208">
        <property type="protein sequence ID" value="CCZ84288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00027"/>
    <dbReference type="EnsemblBacteria" id="CCZ84288">
        <property type="protein sequence ID" value="CCZ84288"/>
        <property type="gene ID" value="BN566_00027"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="303"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="339"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="343"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="397" mass="43197" checksum="F02642DD0F4217CF" modified="2013-07-24" version="1">MKRMGKVIGSSLFSLLLLLGLVLPMQTTVPVLQPMTVAANDLTGHLPATGEVKGDGVYGYTTNYVCYGGAIEEQHAVQNGWHITLKNAHYSHGVQWFECWDTDDGDYYGWIDWKYITVYSSTIKDEPSSGSSPVTITSISPRKGQVAGNGVYGYASSYACYSDGAQATQHKVENAWHITAKNTCSSHGVTWYECWDTDDGDYYGWIDSNYLSFYDTTPAPAQTQAPKQTVIVEKTVLVTEPPKETVVVEKTILVTEPPTETMIVENTVLVTETKEPTTTKVVAAVNLDDQSKDDSSKENDSSWLGLLLIGAGILVLAGLAGALIVSYAKRSKEKAAGSVQFHSPWEDQDSPMNPYAGESQNLIPGNHYPGSSVCPNCGTICTDPNAKFCERCGTLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1Q2</accession>
    <name>R5V1Q2_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Stage 0 sporulation protein A homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN566_00377</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000239">
        <property type="protein sequence ID" value="CCZ84478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00377"/>
    <dbReference type="EnsemblBacteria" id="CCZ84478">
        <property type="protein sequence ID" value="CCZ84478"/>
        <property type="gene ID" value="BN566_00377"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3 8 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Response regulatory" evidence="9">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="10">
        <location>
            <begin position="127"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="8">
        <location>
            <begin position="127"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="7">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ84478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="226" mass="25723" checksum="1559A9039E0781A1" modified="2013-07-24" version="1">MIYFLEDDDSIRELVIYTLRNTGMEAMGFPRPSAFWEAMQQEELPALVLLDIMLPEEDGVSVLKKLRANRATKTLPVIMLTAKGTEYDQVMGLDYGADDYIPKPFGMMALIARIKAVLRRSAPSEEEQEYVLGDLYVNPTRHIVRVGKEPVSLTLKEFELLCLLLEHQGVVFTRDRLLNHIWGYAFDGESRTVDVHVRHLRQKLGECGKYIETVRGIGYRAGDGES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1T3</accession>
    <name>R5V1T3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SHSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00184</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000201">
        <property type="protein sequence ID" value="CCZ84255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00184"/>
    <dbReference type="EnsemblBacteria" id="CCZ84255">
        <property type="protein sequence ID" value="CCZ84255"/>
        <property type="gene ID" value="BN566_00184"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="SSF49764"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SHSP" evidence="3">
        <location>
            <begin position="14"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="127" mass="14650" checksum="ED1050F675EBC9E7" modified="2013-07-24" version="1">MLNAFHDFENDFFAGTGLSSCKVDVRDEGDKFVMEAELPGFEKDEIHLDVEDDCLKLSAEHHTDSEDKDDKKKYIRKERSEVSYQRSFDLTGIDAEKLDAEYKNGILYVTLPKQQPETPTSKRLEVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5V285</accession>
    <name>R5V285_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_00337</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000225">
        <property type="protein sequence ID" value="CCZ84430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00337"/>
    <dbReference type="EnsemblBacteria" id="CCZ84430">
        <property type="protein sequence ID" value="CCZ84430"/>
        <property type="gene ID" value="BN566_00337"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010619">
        <property type="entry name" value="ThrE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024528">
        <property type="entry name" value="ThrE_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06738">
        <property type="entry name" value="ThrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12821">
        <property type="entry name" value="ThrE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="226"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="260"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="296"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="347"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="379"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="407"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="ThrE" evidence="6">
        <location>
            <begin position="36"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="ThrE_2" evidence="7">
        <location>
            <begin position="303"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF06738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ84430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="440" mass="47501" checksum="FC98717EA2DD8754" modified="2013-07-24" version="1">MAKLKKQHMTIHWKAIVQDDSSIPATEATLKEKATLVGRIGLMMLSVGTGAWRVRNSMNKVARCLGITCTADIGLLAISYTCIEGTDTYTQSISNPTTGVNTHKLMQLELFMKEFPDKVSTYSVKHIHEVLDEIQKLPANYKAWNLGLASALACGAFTFLLGGGPVEMCCAFLGAGVGNFVRKKMLGHSISLLANVAVSVAASCLVYVLTILLAQSLLSVSAVHQAGYICAMLFVIPGFPLITGGIDLAKLDLRSGLERVSYALLIILTATMTGWATAAICQFQPADFEPLQLQKWTLLCFRLITSFCGVYGFSLMFNSPRKMAFTAALIGMFTNTLRLELVDLAHMPIGAAAFIGAFCTGCIASVVKKKIGFPRISLTVPAIVIMVPGLYMYRGIYYIALNDVGSGALWLTKAALIVIALPMGLVAARICTDKNFRHCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5V2B0</accession>
    <name>R5V2B0_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyl-gamma-glutamyl-phosphate reductase</fullName>
            <shortName evidence="1">AGPR</shortName>
            <ecNumber evidence="1">1.2.1.38</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">N-acetyl-glutamate semialdehyde dehydrogenase</fullName>
            <shortName evidence="1">NAGSA dehydrogenase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">argC</name>
        <name evidence="3" type="ORF">BN566_00536</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the NADPH-dependent reduction of N-acetyl-5-glutamyl phosphate to yield N-acetyl-L-glutamate 5-semialdehyde.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>N-acetyl-L-glutamate 5-semialdehyde + NADP(+) + phosphate = H(+) + N-acetyl-L-glutamyl 5-phosphate + NADPH</text>
            <dbReference type="Rhea" id="RHEA:21588"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29123"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57936"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.38"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 3/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAGSA dehydrogenase family. Type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.38" evidence="1"/>
    <dbReference type="EMBL" id="CBAR010000267">
        <property type="protein sequence ID" value="CCZ84683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00536"/>
    <dbReference type="EnsemblBacteria" id="CCZ84683">
        <property type="protein sequence ID" value="CCZ84683"/>
        <property type="gene ID" value="BN566_00536"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00108"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003942">
        <property type="term" value="F:N-acetyl-gamma-glutamyl-phosphate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01110">
        <property type="entry name" value="ArgC_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010136">
        <property type="entry name" value="AGPR_type-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000534">
        <property type="entry name" value="Semialdehyde_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01118">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00859">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01851">
        <property type="entry name" value="argC_other"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="1" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Semialdhyde_dh" evidence="2">
        <location>
            <begin position="4"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="315" mass="34460" checksum="1EC7C6F15B6877FB" modified="2013-07-24" version="1">MGVNVFIDGQAGTTGLRILNRFANRNDITLLQIDPEKRKDAAERKRLINSSDYTFLCLPDAAAREAVSLIENPDVRVIDASTAHRINPDWAYGFPELSAAHREKIRTSKRVAVPGCYASGFNALVYPMVQHGIIGADYPVQCFAVSGYSGGGNKMIAEIQSPDCPEESKSPRFYGLANNHKHLPEMQKISRLANKPMFTPIVDDFFNGMVVCVSVQSAIAGHSVTPEAVHAMYAEHYQGQNFVKVMPLLTSEEMTPFKLSSNDLRDTNLMQIFVFGSADQVLLCARLDNLGKGASGAAVQCLNIMMGIDETTGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5V2L1</accession>
    <name>R5V2L1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ADP-ribose pyrophosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00051</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000241">
        <property type="protein sequence ID" value="CCZ84505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00051"/>
    <dbReference type="EnsemblBacteria" id="CCZ84505">
        <property type="protein sequence ID" value="CCZ84505"/>
        <property type="gene ID" value="BN566_00051"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="137" mass="15872" checksum="A0DACABCBF7DAB03" modified="2013-07-24" version="1">MMHEKSCGAIVYRKSHGNIEILLIKHVNSGHWSFPKGHVEGTETEVETAKREIKEETAIDVLIDPTFRETVSYFPKRDTQKTVVYFIARAKNYDFFPQAEEISEIRWVDIGHASNILTYENDKNIVTKAKVAIREFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>R5V333</accession>
    <name>R5V333_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">Ornithine carbamoyltransferase</fullName>
            <shortName evidence="5">OTCase</shortName>
            <ecNumber evidence="3 5">2.1.3.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN566_00543</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>carbamoyl phosphate + L-ornithine = H(+) + L-citrulline + phosphate</text>
            <dbReference type="Rhea" id="RHEA:19513"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="EC" id="2.1.3.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the aspartate/ornithine carbamoyltransferase superfamily. OTCase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.3" evidence="3 5"/>
    <dbReference type="EMBL" id="CBAR010000267">
        <property type="protein sequence ID" value="CCZ84690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00543"/>
    <dbReference type="EnsemblBacteria" id="CCZ84690">
        <property type="protein sequence ID" value="CCZ84690"/>
        <property type="gene ID" value="BN566_00543"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004585">
        <property type="term" value="F:ornithine carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01109">
        <property type="entry name" value="OTCase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002292">
        <property type="entry name" value="Orn/put_carbamltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024904">
        <property type="entry name" value="OTCase_ArgI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00102">
        <property type="entry name" value="OTCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="SSF53671"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00658">
        <property type="entry name" value="orni_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00097">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="OTCace_N" evidence="8">
        <location>
            <begin position="2"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="OTCace" evidence="7">
        <location>
            <begin position="148"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Carbamoyl phosphate binding" evidence="5">
        <location>
            <begin position="51"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Carbamoyl phosphate binding" evidence="5">
        <location>
            <begin position="129"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Ornithine binding" evidence="5">
        <location>
            <begin position="224"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Carbamoyl phosphate binding" evidence="5">
        <location>
            <begin position="260"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ84690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="301" mass="33556" checksum="AABEC933AF779A2A" modified="2013-07-24" version="1">MKHLLKMLDLSGEEILEILDLADQLKYELKHSIPHPYLKGKSIGLIFQKASTRTRVSFETGMYQLGGHALFLSASDLQIGRGEPVQDTARVLSRYLNGIMIRTFEQKEVEDLAEYGSIPVINGLTDFSHPCQVLADLMTIRERKSTFDGLKMCFIGDGNNMANSLIVGGLKVGMEVSIACPEGYHPDPQVLEFAKAYGDKFTLTDQPLEAAKDADVVITDVWASMGQEGEAEKRKVAFNGYQINDDVMAQAHADAMVLHCLPAHREEEITEKVFEAHADEIFEEAENRLHAQKAVMVKLMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5V3T9</accession>
    <name>R5V3T9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transporter putative fucose permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN566_00569</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000004">
        <property type="protein sequence ID" value="CCZ82764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00569"/>
    <dbReference type="EnsemblBacteria" id="CCZ82764">
        <property type="protein sequence ID" value="CCZ82764"/>
        <property type="gene ID" value="BN566_00569"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="155"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="211"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="244"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="275"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="333"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="365"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="8">
        <location>
            <begin position="4"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ82764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="390" mass="41773" checksum="6D6C39A53D8D5F65" modified="2013-07-24" version="1">MTILISIIYLAFISLGLPDSLLGSAWPVMHLELGVATSYAGIITMIISFGTILSSLMSDRLNKKFGTGLVTACSVFLTAAALFGFSCSNSMLALCLWAIPYGLGAGAVDAALNNYVALHYSSRQMSWLHCMWGVGAAISPYIMSFCLTRQLGWQQGYRTVGILQIILTVILICSLSLWKRVAVRKPEATAETPSAKSIGVLQAVRIKGVPMVLLAFFAYCALEQTAMLWASSYLVQNRGLEAETAAQFAAMFLIGITVGRFLSGFLADRWGDRNMIRCGAAIAIAGILLVLIPVSSPLPAQIGLMITGLGCAPIYPAIIHATPAHFGAEYSQAIIGIQMACAYVGTTFMPPLFGVLAGKIGIGCYPVYLLLFAVLMVGMTEWLNRTVSKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>R5V478</accession>
    <name>R5V478_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Acetolactate synthase</fullName>
            <ecNumber evidence="4 8">2.2.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BN566_00724</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>H(+) + 2 pyruvate = (2S)-2-acetolactate + CO2</text>
            <dbReference type="Rhea" id="RHEA:25249"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="2.2.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
        <text evidence="8">Binds 1 thiamine pyrophosphate per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the TPP enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.6" evidence="4 8"/>
    <dbReference type="EMBL" id="CBAR010000029">
        <property type="protein sequence ID" value="CCZ82924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00724"/>
    <dbReference type="EnsemblBacteria" id="CCZ82924">
        <property type="protein sequence ID" value="CCZ82924"/>
        <property type="gene ID" value="BN566_00724"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00055"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00059"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd02015">
        <property type="entry name" value="TPP_AHAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012846">
        <property type="entry name" value="Acetolactate_synth_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039368">
        <property type="entry name" value="AHAS_TPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045229">
        <property type="entry name" value="TPP_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968">
        <property type="entry name" value="PTHR18968"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="SSF52467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00118">
        <property type="entry name" value="acolac_lg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TPP_enzyme_N" evidence="11">
        <location>
            <begin position="4"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="TPP_enzyme_M" evidence="9">
        <location>
            <begin position="191"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="TPP_enzyme_C" evidence="10">
        <location>
            <begin position="385"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CCZ82924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="560" mass="59623" checksum="578534CADDB1917A" modified="2013-07-24" version="1">MKLNGAQIVIETLIEQGTTTVFGYPGGQVINIYDALYLASDRIHHIITAHEQGASHAADGYARATGKVGVVIATSGPGATNLVTGIATAYLDSVPLVAITGNVPNSLIGRDSFQEVDITGVTMPITKHNFFVKKIEDLAPTIREAFRIAKSGRPGPVLVDIPKDVQTALYEYNVEPVVEKAPVPEVDPKLLKQAAELIATAKRPYLYIGGGAITDNAGEEILALADKMDAPIGCTMMGISAVPTDHPRYLGMEGMHGHYASSIAQNEADLIVTAGCRFSDRGTGNTAKYAKHAKIIHIDIDASELNKNITVELGIAGSMKQILAELTAMVPQQQHPEWDARIQDLVAITKELEAQKDDELTPFTALDAVAEAAKNANDDVIVATDVGQHQMWTAQRYPFSKPRTFISSGGLGTMGFGLGAAIGAATGTGKKTVLVTGDGSFGMNLNELATAVSNQTPVVVFIMNNGVLGMVRQWQTLFFDKHYSNTTLERKTDFVKLAEAFGLPGFRAASLPELKEVLKQAFAVQGPVLVDCAIDCDAFVLPMLPPGGAVEDIITDVSAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5V4B0</accession>
    <name>R5V4B0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00749</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000032">
        <property type="protein sequence ID" value="CCZ82949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82949">
        <property type="protein sequence ID" value="CCZ82949"/>
        <property type="gene ID" value="BN566_00749"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="132" mass="14095" checksum="44EB4C7691E7A0D0" modified="2013-07-24" version="1">MSKEAKSEVADNSADTVMDGVLSENQAKLNKLNSSIQAAYERRRKIITDSKLYIYDKLSELYGGAEGQALLDAVTAEHTLIGKLTASGMSYADIEGLVDSSDGADSKSGNSSDNDVADGQTSFFENENPYAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>R5V4F6</accession>
    <name>R5V4F6_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribonuclease P protein component</fullName>
            <shortName evidence="6">RNase P protein</shortName>
            <shortName evidence="6">RNaseP protein</shortName>
            <ecNumber evidence="6">3.1.26.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Protein C5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rnpA</name>
        <name evidence="7" type="ORF">BN566_00106</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">RNaseP catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus. It can also cleave other RNA substrates such as 4.5S RNA. The protein component plays an auxiliary but essential role in vivo by binding to the 5'-leader sequence and broadening the substrate specificity of the ribozyme.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Consists of a catalytic RNA component (M1 or rnpB) and a protein subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the RnpA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="6"/>
    <dbReference type="EMBL" id="CBAR010000035">
        <property type="protein sequence ID" value="CCZ82984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00106"/>
    <dbReference type="EnsemblBacteria" id="CCZ82984">
        <property type="protein sequence ID" value="CCZ82984"/>
        <property type="gene ID" value="BN566_00106"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00227">
        <property type="entry name" value="RNase_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000100">
        <property type="entry name" value="RNase_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020539">
        <property type="entry name" value="RNase_P_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33992">
        <property type="entry name" value="PTHR33992"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00825">
        <property type="entry name" value="Ribonuclease_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00188">
        <property type="entry name" value="rnpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00648">
        <property type="entry name" value="RIBONUCLEASE_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ82984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="132" mass="15369" checksum="BD6E2F2FFB92B4D4" modified="2013-07-24" version="1">MKQTIILNQNRDFQTLYKRGISFVTPFVVIYMRPNRFGVHRLGITAGKRVGNAVQRNRAKRLIRQAYRELEAELPPYLDIVIVARARICNLTSTRLVKYLRKETIPQIQRHWEPPIRMRAGGKSSKPEGTPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5V4N1</accession>
    <name>R5V4N1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Bacterial cell division membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_00837</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000040">
        <property type="protein sequence ID" value="CCZ83044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00837"/>
    <dbReference type="EnsemblBacteria" id="CCZ83044">
        <property type="protein sequence ID" value="CCZ83044"/>
        <property type="gene ID" value="BN566_00837"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001182">
        <property type="entry name" value="FtsW/RodA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30474">
        <property type="entry name" value="PTHR30474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01098">
        <property type="entry name" value="FTSW_RODA_SPOVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0133">Cell shape</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="120"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="149"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="172"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="274"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="311"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="345"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ83044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="390" mass="42978" checksum="9D485D5FCEBB02F7" modified="2013-07-24" version="1">MKTVLQAVAQYWKKLDKPLLFAAMLCSALSVVLLYSIYINHTLSEIDDDDYIIQLVAVGIGVLSVLVLAAIDYHKIARLWFVYAPIALILVLLTFTSLGYRRAGADDQAWLQLGSVTLQPSEILKLAFILTFSLHLAKDEENMNRPLHMLLLILHGCMPIGLVFLQGDYGTAVVFAFIFAAMMLIAHISWKYVLAGMIAVPAALVVAWNFVLGSLHKNRILVLLHPGTDPEGLEYQQDLGLSALASGKLFGVGLFDGDSYISVPEMHNDFIFSYIGQTLGFVGAMAVVLVMVFLCLRTLANGLRANDRQGMFLCIGVSSMIFTHCFMNIGMVLKVMPVIGVPLPFLSAGGTSTLSMYIAIGMVISVRTHNERKYNVFYDYKNPTLARVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5V538</accession>
    <name>R5V538_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Nitrogen regulatory protein P-II</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01020</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000081">
        <property type="protein sequence ID" value="CCZ83239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01020"/>
    <dbReference type="EnsemblBacteria" id="CCZ83239">
        <property type="protein sequence ID" value="CCZ83239"/>
        <property type="gene ID" value="BN566_01020"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006808">
        <property type="term" value="P:regulation of nitrogen utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002187">
        <property type="entry name" value="N-reg_PII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30115">
        <property type="entry name" value="PTHR30115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00543">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00340">
        <property type="entry name" value="PIIGLNB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00938">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="SSF54913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51343">
        <property type="entry name" value="PII_GLNB_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="118" mass="13355" checksum="E411BE7BB476C8F9" modified="2013-07-24" version="1">MIIKVEAIIREEKLQDVLDALGEMEVHGLTCYQVMGCGTQRGMKSYVRGHQEVEIHMLPKIKLEVLVSSEEWEKKTMETIQKAAYTGNIGDGKIFSYDVRDAMRIRTKETGPGAIQPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5V580</accession>
    <name>R5V580_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transcriptional regulator BadM/Rrf2 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01065</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01065"/>
    <dbReference type="EnsemblBacteria" id="CCZ83284">
        <property type="protein sequence ID" value="CCZ83284"/>
        <property type="gene ID" value="BN566_01065"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000944">
        <property type="entry name" value="Tscrpt_reg_Rrf2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33221">
        <property type="entry name" value="PTHR33221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02082">
        <property type="entry name" value="Rrf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00738">
        <property type="entry name" value="rrf2_super"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51197">
        <property type="entry name" value="HTH_RRF2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="148" mass="16928" checksum="D01CBDF03898AAFE" modified="2013-07-24" version="1">MKVSTRVECGILALTDIAMHQTEGKPVTVSSISNRQDLSMKYLEQILMVLRQSNLVRSTKGFKGGYVLTRPAKEITLRTVIDALDVTVLADVHFQEESETPLKTIVNHQLWNPMTTYLRKFCDSITISDMMEQYRTFLSDTEGPMYYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>R5V6H1</accession>
    <name>R5V6H1_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Probable GTP-binding protein EngB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">engB</name>
        <name evidence="10" type="ORF">BN566_01459</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Necessary for normal cell division and for the maintenance of normal septation.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. EngB GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000158">
        <property type="protein sequence ID" value="CCZ83724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01459"/>
    <dbReference type="EnsemblBacteria" id="CCZ83724">
        <property type="protein sequence ID" value="CCZ83724"/>
        <property type="gene ID" value="BN566_01459"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000917">
        <property type="term" value="P:division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01876">
        <property type="entry name" value="YihA_EngB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00321">
        <property type="entry name" value="GTPase_EngB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030393">
        <property type="entry name" value="G_ENGB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019987">
        <property type="entry name" value="GTP-bd_ribosome_bio_YsxC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03598">
        <property type="entry name" value="GTPase_YsxC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51706">
        <property type="entry name" value="G_ENGB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="6 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0717">Septation</keyword>
    <feature type="domain" description="EngB-type G" evidence="9">
        <location>
            <begin position="22"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ83724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="200" mass="22655" checksum="50AD3D32D36CFDC8" modified="2013-07-24" version="1">MNFQQATFLASYGTFSQLPPCKGMEIAFAGRSNVGKSTMLNKIFNRKALARVSAVPGKTATINFYQIPPVTFVDLPGYGYAKVAKTEKRRWAELIDGYLESDRDIRLTFLLLDMRHAPSKDDLQMIEYLIAQEMPFVIVLTKADKLNKSQRKARMEAFQTEIPYFSDIHVIPFSAMTREGVDEVRNVITELAEADETDAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5V6L9</accession>
    <name>R5V6L9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Bis(5'-nucleosyl)-tetraphosphatase (symmetrical)</fullName>
            <ecNumber evidence="2">3.6.1.41</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_01499</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + P(1),P(4)-bis(5'-adenosyl) tetraphosphate = 2 ADP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:24252"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58141"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.1.41"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.41" evidence="2"/>
    <dbReference type="EMBL" id="CBAR010000161">
        <property type="protein sequence ID" value="CCZ83764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01499"/>
    <dbReference type="EnsemblBacteria" id="CCZ83764">
        <property type="protein sequence ID" value="CCZ83764"/>
        <property type="gene ID" value="BN566_01499"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006675">
        <property type="entry name" value="HDIG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005249">
        <property type="entry name" value="YqeK"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00277">
        <property type="entry name" value="HDIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00488">
        <property type="entry name" value="TIGR00488"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HDc" evidence="5">
        <location>
            <begin position="44"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ83764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="224" mass="25662" checksum="A46E34398C82F4AB" modified="2013-07-24" version="1">MAKESVIEKMKHKMQPPPPKEVSISEATESMRKVLEKQVSEETMRKERYEHSVRVAAECKRLAQIYHVNEDLAEFAGMVHDICKDMKADDQKALMEAGKDDLDITEEEWNTKKLWHGIAGAQLMRQKYCMNNQHVLDAIRYHTVARKGMTPLEQIVYLADMTEAGRNFIDVHTLRAAVNQNLNLGMYYALKISIANVLDKGGLLPRHTIEAYNQYVQIVAGMKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5V6U1</accession>
    <name>R5V6U1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Acetyltransferase gnat family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01564</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000165">
        <property type="protein sequence ID" value="CCZ83829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01564"/>
    <dbReference type="EnsemblBacteria" id="CCZ83829">
        <property type="protein sequence ID" value="CCZ83829"/>
        <property type="gene ID" value="BN566_01564"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017255">
        <property type="entry name" value="AcTrfase_GNAT_prd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037663">
        <property type="entry name" value="Acetyltransf_GNAT_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="146" mass="16310" checksum="BAFC4EA799B3ACCC" modified="2013-07-24" version="1">MQIRTMQMEDYDAVFALWKSCSGMGLNDVDDSRSGIERFLQRNPETCLVAVEETAIIGAILVGSDGRRAYIYHTAVAPERQKQGIGSQLVQAAINAVQKIGIAKMALVVFSRNQSGNAFWEKQGFSVREDLTYRNLALQEMTRMDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>R5V6W6</accession>
    <name>R5V6W6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cell division protein ZapA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01584</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000167">
        <property type="protein sequence ID" value="CCZ83849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01584"/>
    <dbReference type="EnsemblBacteria" id="CCZ83849">
        <property type="protein sequence ID" value="CCZ83849"/>
        <property type="gene ID" value="BN566_01584"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007838">
        <property type="entry name" value="Cell_div_ZapA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036192">
        <property type="entry name" value="Cell_div_ZapA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05164">
        <property type="entry name" value="ZapA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102829">
        <property type="entry name" value="SSF102829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="56"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="121" mass="13276" checksum="37C54143DB89295C" modified="2013-07-24" version="1">MTNKVKVVICGKDFMLQTAESANYVIGLARTLENRISEVSGSNSSTSPFAAAIMVALSTLDDLNKATQRLDALREQAKEYVDEAGKNRLAYDNAIAENKQLLARIEALEKELQNNRQTANA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5V6Y3</accession>
    <name>R5V6Y3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">LPG_synthase_C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_00169</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000168">
        <property type="protein sequence ID" value="CCZ83864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00169"/>
    <dbReference type="EnsemblBacteria" id="CCZ83864">
        <property type="protein sequence ID" value="CCZ83864"/>
        <property type="gene ID" value="BN566_00169"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024320">
        <property type="entry name" value="LPG_synthase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016732">
        <property type="entry name" value="UCP018688"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41373">
        <property type="entry name" value="PTHR41373"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09924">
        <property type="entry name" value="LPG_synthase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018688">
        <property type="entry name" value="UCP018688"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="LPG_synthase_C" evidence="7">
        <location>
            <begin position="23"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF09924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="300" mass="34254" checksum="C5DA218D6D86F38A" modified="2013-07-24" version="1">MLTFRTISISDREWIQKALQQSQYQGCEYSFANNLAWYRGSDSRIASFQGFYIIAAFDTPDGIPDVTVPSGSGDWDALLRELVETLCPNGEPLRFTGVTREMAAFLQQRFPDAVWQPDPAAYDYIYRTEDLITLKGKRYHKKRNHLHQFQERYNWTFTPLTAADQDACIAFSAALYNEKKGYDDRSSIVEQFAIHTFFTHWDTLGLCGGVLRVDGKLVGFSIGEPLNRDTFVTHIEKADIAYQGSYTALTQAFTAQFAKNYRYINREEDLGIAGLRQAKASYYPVYLLEKGDLVIPASMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7A4</accession>
    <name>R5V7A4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Riboflavin biosynthesis protein RibBA</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="12">3,4-dihydroxy-2-butanone 4-phosphate synthase</fullName>
                <shortName evidence="12">DHBP synthase</shortName>
                <ecNumber evidence="12">4.1.99.12</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="12">GTP cyclohydrolase-2</fullName>
                <ecNumber evidence="12">3.5.4.25</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="12">GTP cyclohydrolase II</fullName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="12" type="primary">ribBA</name>
        <name evidence="14" type="ORF">BN566_01703</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 12">Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.</text>
    </comment>
    <comment type="function">
        <text evidence="12">Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>D-ribulose 5-phosphate = (2S)-2-hydroxy-3-oxobutyl phosphate + formate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18457"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
            <dbReference type="EC" id="4.1.99.12"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + formate + 3 H(+) + 2 phosphate</text>
            <dbReference type="Rhea" id="RHEA:23704"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58614"/>
            <dbReference type="EC" id="3.5.4.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="12">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="12">Binds 2 divalent metal cations per subunit. Magnesium or manganese.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="12">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 12">Cofactor biosynthesis; riboflavin biosynthesis; 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-6-(D-ribitylamino)uracil from GTP: step 1/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">In the C-terminal section; belongs to the GTP cyclohydrolase II family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 12">In the N-terminal section; belongs to the DHBP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.12" evidence="12"/>
    <dbReference type="EC" id="3.5.4.25" evidence="12"/>
    <dbReference type="EMBL" id="CBAR010000178">
        <property type="protein sequence ID" value="CCZ83974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01703"/>
    <dbReference type="EnsemblBacteria" id="CCZ83974">
        <property type="protein sequence ID" value="CCZ83974"/>
        <property type="gene ID" value="BN566_01703"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00399"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008686">
        <property type="term" value="F:3,4-dihydroxy-2-butanone-4-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003935">
        <property type="term" value="F:GTP cyclohydrolase II activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00641">
        <property type="entry name" value="GTP_cyclohydro2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00179">
        <property type="entry name" value="RibA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01283">
        <property type="entry name" value="RibBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000422">
        <property type="entry name" value="DHBP_synthase_RibB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032677">
        <property type="entry name" value="GTP_cyclohydro_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000926">
        <property type="entry name" value="RibA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036144">
        <property type="entry name" value="RibA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016299">
        <property type="entry name" value="Riboflavin_synth_RibBA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00926">
        <property type="entry name" value="DHBP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00925">
        <property type="entry name" value="GTP_cyclohydro2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142695">
        <property type="entry name" value="SSF142695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="SSF55821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00505">
        <property type="entry name" value="ribA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00506">
        <property type="entry name" value="ribB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0456">Lyase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0464">Manganese</keyword>
    <keyword evidence="7 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="8 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0686">Riboflavin biosynthesis</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="GTP_cyclohydro2" evidence="13">
        <location>
            <begin position="217"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="DHBP synthase" evidence="12">
        <location>
            <begin position="1"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="D-ribulose 5-phosphate binding" evidence="12">
        <location>
            <begin position="27"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="D-ribulose 5-phosphate binding" evidence="12">
        <location>
            <begin position="139"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="GTP cyclohydrolase II" evidence="12">
        <location>
            <begin position="210"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for GTP cyclohydrolase activity" evidence="12">
        <location>
            <position position="337"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; for GTP cyclohydrolase activity" evidence="12">
        <location>
            <position position="339"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="28"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>D-ribulose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="260"/>
            <end position="264"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="303"/>
            <end position="305"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="325"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="360"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="365"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="site" description="Essential for DHBP synthase activity" evidence="12">
        <location>
            <position position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CCZ83974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="420" mass="46390" checksum="274B1D866112A346" modified="2013-07-24" version="1">MNYHTIEEALDALRAGKCILVTDDPDRENEGDLICAGQFATTENVNLMASAAKGLICMPMSAANVERLGLSQMVQHNTDNHETAFTVSIDHVDTTTGISAVERGLTARMAADQNSCPADFRRPGHMFPLTAKAGGVLERNGHTEATVDLLRLAGLTQVGLLAGLTQVGLCCEIMAEDGTMMRGEQLMTFAKEHDLVFITIRELQRYRRQHDQFVQEVADAHLPTAYGDFQIHGFRNTINGEEHVALVMGDVADGNPVLCRVHSECLTGDVFGSCRCDCGEQLQKALQCIQKEGRGILVYLRQEGRGIGLLNKIKAYKLQEEGLDTVEANIQLGFAPDLRDYSVGAQILKELGATKLRLMTNNPEKLTDLSEYGITITERVPLDIPEKPENIRYLQTKQFKMGHYLHFRTCGNSIQKPKAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7U6</accession>
    <name>R5V7U6_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Histidinol-phosphate aminotransferase</fullName>
            <ecNumber evidence="5">2.6.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Imidazole acetol-phosphate transaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">hisC</name>
        <name evidence="7" type="ORF">BN566_01914</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>2-oxoglutarate + L-histidinol phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:23744"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57766"/>
            <dbReference type="ChEBI" id="CHEBI:57980"/>
            <dbReference type="EC" id="2.6.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 7/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. Histidinol-phosphate aminotransferase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.9" evidence="5"/>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01914"/>
    <dbReference type="EnsemblBacteria" id="CCZ84189">
        <property type="protein sequence ID" value="CCZ84189"/>
        <property type="gene ID" value="BN566_01914"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00012"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004400">
        <property type="term" value="F:histidinol-phosphate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01023">
        <property type="entry name" value="HisC_aminotrans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001917">
        <property type="entry name" value="Aminotrans_II_pyridoxalP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005861">
        <property type="entry name" value="HisP_aminotrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01141">
        <property type="entry name" value="hisC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00599">
        <property type="entry name" value="AA_TRANSFER_CLASS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3 5 7" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="4 5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="6">
        <location>
            <begin position="25"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="5">
        <location>
            <position position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ84189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="353" mass="39787" checksum="7CF31693AA57E70E" modified="2013-07-24" version="1">MSAAFMNYVRKVDPYVPGDQPNFPDMVKLNTNENPYPPAPAVEAALHDLSVDSLRLYPDPVVSKLNTAIADFYGVKPEQVFSGVGSDDVLAMCFLTFFNSKKPIFFPDVTYGFYPVWSELFQIPYEKKPLNDRFELVKEDYYAENGGIVFPNPNAPTGIALSVADIEDIIQHNPDSIVIVDEAYIDFGGTSVLPLIDKYDNLIVVQTFSKSRSMAGMRIGFAVSNPTLIRVLNDCKFSFNSYTMNQTTIAAGVAAVSDRAYLEETLAKIVATREHAKKVLAEMGFQFPDSQSNFLFVTHPDYSAKELYEMLKEQHIFVRYFGTGRTKDYLRITIGTEEQMETLYAALRAYFQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5V819</accession>
    <name>R5V819_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transport permease protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_00203</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ABC-2 integral membrane protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000205">
        <property type="protein sequence ID" value="CCZ84274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00203"/>
    <dbReference type="EnsemblBacteria" id="CCZ84274">
        <property type="protein sequence ID" value="CCZ84274"/>
        <property type="gene ID" value="BN566_00203"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC_2_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000412">
        <property type="entry name" value="ABC_2_transport"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006648">
        <property type="entry name" value="DrrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00164">
        <property type="entry name" value="ABC2TRNSPORT"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51012">
        <property type="entry name" value="ABC_TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="30"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="62"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="108"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="136"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="173"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="226"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="ABC2_membrane" evidence="7">
        <location>
            <begin position="14"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ84274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="258" mass="29590" checksum="D4CD2B276DF7E987" modified="2013-07-24" version="1">MKLFRELYHYREMLFSLVRKDLRGRYKGSVLGFLWTFINPLMQLLVYTFVFTVILPAQVEHYSLYLFVALIPWIFFSAAMTGGAGSIVAQKDLVKKIYFPREIIPISYVTSCFVNMLLCFLIIFLVMLVTGATFNLLAFLCLPIIMVVEYLLALGIAMLSAAITVYFRDLEHILGIVSMIWMYMTPVLYPETLVPERFQKLYHLNPMTSIITAYRDVLYRGVVPQLSTLLEAVILGILFLAVGLLTFSKLKKGFAEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5V868</accession>
    <name>R5V868_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00237</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000213">
        <property type="protein sequence ID" value="CCZ84324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84324">
        <property type="protein sequence ID" value="CCZ84324"/>
        <property type="gene ID" value="BN566_00237"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="195" mass="21722" checksum="F2DBF4F5580E1917" modified="2013-07-24" version="1">MGLGHFHQKQTGIQTPTPPVLENVVYYAQGDPKWKQDALGDSVYHMGDSGCLVTCLAAALQMQNIHRTALGDDINPKTLNQFFSAHQVYDAEGDLQWYAMEDALQVSTVYLESGNFSVETLENLWKETIYPIACVSGKHGNLHFVLLVGRTEDENWCMDPMHTEPELVPLSQYGNDIASIRYLIEPSSSESKAAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5V8N6</accession>
    <name>R5V8N6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Peptidase M22 glycoprotease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00368</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000238">
        <property type="protein sequence ID" value="CCZ84469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00368"/>
    <dbReference type="EnsemblBacteria" id="CCZ84469">
        <property type="protein sequence ID" value="CCZ84469"/>
        <property type="gene ID" value="BN566_00368"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002949">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000905">
        <property type="entry name" value="Gcp-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022496">
        <property type="entry name" value="T6A_TsaB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735">
        <property type="entry name" value="PTHR11735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735:SF11">
        <property type="entry name" value="PTHR11735:SF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00814">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03725">
        <property type="entry name" value="T6A_YeaZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TsaD" evidence="1">
        <location>
            <begin position="33"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="226" mass="24405" checksum="E9DCD788BA1C421B" modified="2013-07-24" version="1">MRYLGMDTSGSVAAVALYDTEQDLFLSQQSLYTKKTHSQVILPLVERTLQDCGLTAADLDGIAVAVGPGSYTGLRIGVAAVKAMTFALQLPCFGISTLEALAYQAATANGLCSAVMKARQNLVYAGLYQNQNGILQNLVPDGLYEQTEWQQILEQQQAPVLVTGDAAASFKTEQVQLAPASVRLQNATGLCLAACHHEPQTPEQLEANYLQLVKAEKDRQMQQKQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5V8W7</accession>
    <name>R5V8W7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">50S ribosomal protein L30</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_00075</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL30 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000241">
        <property type="protein sequence ID" value="CCZ84529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00075"/>
    <dbReference type="EnsemblBacteria" id="CCZ84529">
        <property type="protein sequence ID" value="CCZ84529"/>
        <property type="gene ID" value="BN566_00075"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01658">
        <property type="entry name" value="Ribosomal_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036919">
        <property type="entry name" value="L30_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005996">
        <property type="entry name" value="Ribosomal_L30_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016082">
        <property type="entry name" value="Ribosomal_L30_ferredoxin-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00327">
        <property type="entry name" value="Ribosomal_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002211">
        <property type="entry name" value="Ribosomal_L30_bac-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55129">
        <property type="entry name" value="SSF55129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01308">
        <property type="entry name" value="rpmD_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal_L30" evidence="5">
        <location>
            <begin position="5"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ84529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="58" mass="6212" checksum="99CBC6AC9820C780" modified="2013-07-24" version="1">MAKQIKLVKSLSGRKKDQVATANSLGLKKIGDVTVQPENAATLGKIQKISHLVEVQEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>R5V968</accession>
    <name>R5V968_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Hexosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00507</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000266">
        <property type="protein sequence ID" value="CCZ84654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00507"/>
    <dbReference type="EnsemblBacteria" id="CCZ84654">
        <property type="protein sequence ID" value="CCZ84654"/>
        <property type="gene ID" value="BN566_00507"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="217"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="400" mass="45614" checksum="E3A184C19A7C45AD" modified="2013-07-24" version="1">MYKIAFVIPWYGEGIPGGAEMELREVAGHLQKAGVEVEVLTTCIKDFTADWNQNYYAAGVAMVYDVPTRRFPVRKRDTATFDRINAQLMQGKHITPKEEVLFLQEMVNSPQLYEYIETHNDDYDLFVFMPYMFGTTFHGVLACPEKAVMIPCFHEEAYAHLRLFRQTYINIRGMIFNSVPEMQLANRLYDLDEVEQICMGIGMDTSISGDGAAFREKYGITDPYLIYAGRKDSGKNVHTLLQYFAEYKKRNPEDPLQLVLIGGGTIAIPDSVKDCVHDLGYVSQQDKYDALQGALFLCQPSKNESFSLVIMESWLCGRPVLVHSACAVTKDFAQQAEGGLYFNSYFEFEGCVQWLEQHSAEAEQLGQNGRQFVLANFDWNVVTKKYLAFFKKLIEEGDMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5V972</accession>
    <name>R5V972_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00512</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000267">
        <property type="protein sequence ID" value="CCZ84659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84659">
        <property type="protein sequence ID" value="CCZ84659"/>
        <property type="gene ID" value="BN566_00512"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="155" mass="18189" checksum="BA8F4AB51F15B57D" modified="2013-07-24" version="1">MLVEKDVSERILSLYDAEDLELCEAYAVDAHLGENLPEFATVYRAEPEHFCFVINPDRIGRNYFKLYNHRFPEEATKMARICFFDASYCKEGSLGKKTFWILNPEEKQQLLQILQRPCEAGNTVWRKLIETFCWEADEERIPLDTPIPDYTKLEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5VGP7</accession>
    <name>R5VGP7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00561</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000003">
        <property type="protein sequence ID" value="CCZ82756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00561"/>
    <dbReference type="EnsemblBacteria" id="CCZ82756">
        <property type="protein sequence ID" value="CCZ82756"/>
        <property type="gene ID" value="BN566_00561"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="267" mass="29621" checksum="A191C2D5D5B16A68" modified="2013-07-24" version="1">MQQTPKTAAEYQAEMMQLYRRAHPETEVPPTPAAEPPAPEIHAVPEISMPPVAPEVPPEPVPEPVSEPQPMPEMPEPQTEPLPEPMAEQQLPEEPQPLPESEEPLITGTGEVLDFPQLETEEVPPPQTLPLEPIPPLTPKTSYGFLKVITRTASEAMPMPNVTVTVSAIVDGESRLFYTTTTNESGETERIPLPAPVQKSEQQPNEIPLFQSYDVSIHYPGYFRMESRSVPIFAGITSLQKFAMIPLPQFSDATTQTIIYENTEPKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>R5VHD9</accession>
    <name>R5VHD9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="12">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">BN566_00756</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000032">
        <property type="protein sequence ID" value="CCZ82956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00756"/>
    <dbReference type="EnsemblBacteria" id="CCZ82956">
        <property type="protein sequence ID" value="CCZ82956"/>
        <property type="gene ID" value="BN566_00756"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="869"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="924"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="962"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="2"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="221"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="221"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CCZ82956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="1002" mass="111405" checksum="24F71D72183F2140" modified="2013-07-24" version="1">MLELKGIKKDYLTGENTVHALKGIDLRFRRNEFVSILGPSGCGKTTMLNIIGGLDQYTEGDLIINGRSTKDFKDRDWDTYRNHSIGFVFQSYNLIPHQTVLQNVELALTLSGVSKAERRKRAKKALEDVGLGNQLSKKPSEMSGGQMQRVAIARALVNNPDIILADEPTGALDTETSIQVMEILKEISKDRLIIMVTHNPELAEQYSSRIIRVLDGKLTDDSKPMTEEEYQQELQADRADNENGKKLKKPSMSFGTSFSLSLKNLFTKKGRTMLTSFAGSIGIIGIALVLSISQGFNTYINVIQEETLSSYPLTIQKTNTDLSALMQTFMGSATSDSTHDKDAVYKKSAIYDMMDALNNMETSENDLKSFKSYIESEREDSDSDLHKAVNGVQYTYDLDLLIYTKNVDGTIIHSDTEELMQEMVLEHMGIDMQAMTNASNSMFGEQATAMMGMGGSSMNLWQELLPGENGNPINDLLKKQYDVVYGSWPNSYDEIMLVLDENNELDDMTLYALGLEPKEDVDNIMEAAVNGKELQKDNKNWSYADICDMDFRTILNADCYRYDESTGLYTDLRDTDAGLQYLYDNGLKLKVTGIIRPNEDTTAPMLSGSIVYTSDLTKYLIDHSKDSDAIAAQKESKDTDIFTGLPFKDSSGSTTDAEKQTAFLEYVNGLDDSGKAQMYIQMMSIPTEEQLNESVQQSMQGMSRSDMEAAMLQGMTQQMSMSESDVQSYLESMSDDEITDTFTQMMQQQVKAQYAQQVQQKMAAMQPEELLKALNQLLPTLTAEQCANYYDELMQFSDSTYEDNLKALGDIDLDDPASINLYAATFEDKDVIEDAIADYNEGKDEMSQIQYTDYVGLMMSSVTKIINTITYVLIAFVAISLIVSSIMIGVITLISVQERTKEIGILRAIGASKRDVSSMFNAETIIIGFTSGLLGVLVTCLLCIPINLLVHHLTNINNLRAILPIPAAIILIAISVLLTLIAGFIPSKSAAKKDPVVALRSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>R5VHU3</accession>
    <name>R5VHU3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">4-hydroxy-3-methylbut-2-enyl diphosphate reductase</fullName>
            <shortName evidence="5">HMBPP reductase</shortName>
            <ecNumber evidence="5">1.17.7.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ispH</name>
        <name evidence="7" type="ORF">BN566_00869</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>H2O + isopentenyl diphosphate + 2 oxidized [2Fe-2S]-[ferredoxin] = (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H(+) + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:24488"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:128753"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="EC" id="1.17.7.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>dimethylallyl diphosphate + H2O + 2 oxidized [2Fe-2S]-[ferredoxin] = (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H(+) + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:24825"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:128753"/>
            <dbReference type="EC" id="1.17.7.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="5">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the IspH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.17.7.4" evidence="5"/>
    <dbReference type="EMBL" id="CBAR010000051">
        <property type="protein sequence ID" value="CCZ83081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00869"/>
    <dbReference type="EnsemblBacteria" id="CCZ83081">
        <property type="protein sequence ID" value="CCZ83081"/>
        <property type="gene ID" value="BN566_00869"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00056">
        <property type="reaction ID" value="UER00097"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00059">
        <property type="reaction ID" value="UER00105"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051745">
        <property type="term" value="F:4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050992">
        <property type="term" value="P:dimethylallyl diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019288">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13944">
        <property type="entry name" value="lytB_ispH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00191">
        <property type="entry name" value="IspH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003451">
        <property type="entry name" value="LytB/IspH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035104">
        <property type="entry name" value="Ribosomal_protein_S1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10724:SF7">
        <property type="entry name" value="PTHR10724:SF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02401">
        <property type="entry name" value="LYTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00681">
        <property type="entry name" value="RIBOSOMALS1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00216">
        <property type="entry name" value="ispH_lytB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3 5" id="KW-0408">Iron</keyword>
    <keyword evidence="4 5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S1 motif" evidence="6">
        <location>
            <begin position="308"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="6">
        <location>
            <begin position="395"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="6">
        <location>
            <begin position="482"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="6">
        <location>
            <begin position="567"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="224"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ83081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="649" mass="71186" checksum="6D467A2D2FC4974B" modified="2013-07-24" version="1">MKQITIAKSAGFCFGVDRAVRMTYEALEQYPHVATLGPIIHNQTVVDDLCQKGARIVEDVSELEPEECIVIRSHGVSKQVEDAIAAAGNPCINATCPFVSKIHKIVEKHTLAGDYILIAGDKNHPEVQAIVGHCGENCTVFADSAALDHFFKTNYENLKKKLAIVAQTTYNILVWERCMALLPSDDPNIMVYDTICHATQVRQSDADTLSRSSDLMIIVGGKHSSNTVKLFDVCKQNCRSYHIETADELYTLDLCNAEKIGITAGASTPAHIIKEVESTMSEIMERHDEEDINFAEALDQSFKKIHKGEKVKGTVAAVNNTEAIVDTGTKYTGYVPLSELTDDPSLKPADVVNVGDELDLIVIQTNDQDGTATLSKKKVDEQKGFEEIVAAKENDTVLEGTVQGVVKGGIIVAYAGVRVFIPASQSGLPRGAELDTLVKTKVRFVILEVNEHRRRAVGSIRAVQKAEKEAAKEKFWETAEVGATYKGEVKSLTSYGAFVDLGGIDGMVHISELSWKRIKHPSEVVKVGDVIEVYIKELDPENNRISLGYKKTEDNPWEKFKATYKVGDIVDATIVSITPFGAFAQIMDGVDGLIHISQLADKRVENVKDIVAIGDVVKVKIIDIDIDNKRISISMRALLEDEAEGEDAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>R5VIB1</accession>
    <name>R5VIB1_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">DNA repair protein RadA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">radA</name>
        <name evidence="8" type="ORF">BN566_01082</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">DNA-dependent ATPase involved in processing of recombination intermediates, plays a role in repairing DNA breaks. Stimulates the branch migration of RecA-mediated strand transfer reactions, allowing the 3' invading strand to extend heteroduplex DNA faster. Binds ssDNA in the presence of ADP but not other nucleotides, has ATPase activity that is stimulated by ssDNA and various branched DNA structures, but inhibited by SSB. Does not have RecA's homology-searching function.</text>
    </comment>
    <comment type="function">
        <text evidence="5">Plays a role in repairing double-strand DNA breaks, probably involving stabilizing or processing branched DNA or blocked replication forks.</text>
    </comment>
    <comment type="domain">
        <text evidence="5">The middle region has homology to RecA with ATPase motifs including the RadA KNRFG motif, while the C-terminus is homologous to Lon protease.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the RecA family. RadA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000083">
        <property type="protein sequence ID" value="CCZ83301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01082"/>
    <dbReference type="EnsemblBacteria" id="CCZ83301">
        <property type="protein sequence ID" value="CCZ83301"/>
        <property type="gene ID" value="BN566_01082"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000725">
        <property type="term" value="P:recombinational repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01121">
        <property type="entry name" value="Sms"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01498">
        <property type="entry name" value="RadA_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004504">
        <property type="entry name" value="DNA_repair_RadA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008269">
        <property type="entry name" value="Lon_proteolytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041166">
        <property type="entry name" value="Rubredoxin_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05362">
        <property type="entry name" value="Lon_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18073">
        <property type="entry name" value="Rubredoxin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00416">
        <property type="entry name" value="sms"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5 6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0346">Stress response</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RECA_2" evidence="7">
        <location>
            <begin position="68"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Lon-protease-like" evidence="5">
        <location>
            <begin position="352"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="RadA KNRFG motif" evidence="5">
        <location>
            <begin position="253"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="97"/>
            <end position="104"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="459" mass="50257" checksum="72767C4C802317D7" modified="2013-07-24" version="1">MAKSKTVFRCQQCGFESPKWNGKCPSCGAWNSLVEETIQPAAAKAPASRKKADLSNFIQELQAVDTQEDIRYQTGIGELDRVLGGGLVKGSIVLLGGEPGIGKSTLLLQICEFFGASHSILYVSGEESTKQIKLRAERLHVTTESLYLLANNDAQSICDTIAACEPDIVIIDSIQTMQITELSSSPGSLTQVRECTNLFMHTAKQHDIPIFIVGHVNKDGAIAGPKVMEHIVDTVLYFEGERNFSYRILRAVKNRFGSTNEIGVFEMMESGLQEVPNPSAMLLEGRPLNVSGTCVTCVMEGSRPILAEVQVLVTKSSLPSPRRTANGFDYNRLFIILAILEKRCGLFYGTLDVYLNVVGGFRLDEPSGDLAVALCLYSSLTDKSISEHVTAFGELGLGGEVRSISNLTQRILEAERMGFQTCIIPKQSMRGLQTSNFQIQLVGVRTLKEAFDFLEQPQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5VIM0</accession>
    <name>R5VIM0_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">M18 family aminopeptidase</fullName>
            <ecNumber evidence="10">3.4.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN566_01172</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the peptidase M18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.-" evidence="10"/>
    <dbReference type="EMBL" id="CBAR010000094">
        <property type="protein sequence ID" value="CCZ83396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01172"/>
    <dbReference type="EnsemblBacteria" id="CCZ83396">
        <property type="protein sequence ID" value="CCZ83396"/>
        <property type="gene ID" value="BN566_01172"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001948">
        <property type="entry name" value="Peptidase_M18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023358">
        <property type="entry name" value="Peptidase_M18_dom2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28570">
        <property type="entry name" value="PTHR28570"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02127">
        <property type="entry name" value="Peptidase_M18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00932">
        <property type="entry name" value="AMINO1PTASE"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9 11" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4 9" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ83396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="469" mass="51055" checksum="080F2B3776FFDE17" modified="2013-07-24" version="1">MAEEKKSESKQLKEKLFASKKHGALRMSEDDQTACEALCKDYMAFLNQSKTEREACANAEALLKEHGFVPFTPGMALKAGDKVYQNNRGKAMIAAIIGSAPITEGVRLCAAHIDSPRLDMKQNPLYEDSELGLFKTHYYGGIKKYQWTAIPLSLHGVIIKADGSKVTVNIGEAEGDPVFCVTDLLPHLATEQMKRPSVGLIRGEELNVLVGSMPFKKSEESEAVKLNLLHILYEKYGITESDFVSAELEAVPAFPVKEIGFDRSMIGGYGHDDRVCSYPALRALLDTISTPKHTAVVILTDKEETGSDGNTGLCSSYLKYFIEDLADCFGAKGRHVLHASQCLSADVNAAFDPTFPDVMERNNCAYLNHGVCVTKFTGARGKSGTSDASAEFVGTVRQLLDKEQVVWQTGELGKVDGGGGGTVAAYIANLDVDTIDVGVPVLCMHAPFEVVAKIDVYMTYRAFFVFMAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VIM7</accession>
    <name>R5VIM7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01177</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000095">
        <property type="protein sequence ID" value="CCZ83401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83401">
        <property type="protein sequence ID" value="CCZ83401"/>
        <property type="gene ID" value="BN566_01177"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="118" mass="12897" checksum="A3246F263BE6DF99" modified="2013-07-24" version="1">MQKIIQAAFPSVDAAEYAASYLQQHIPHVAQVSVTPRNPNSIAIGSIPQTYFSIYTTAAVSAHNITGRIERQLNEEEILEPERNQTADLRISCSAEAYPTAHSILTALGGSQMQNWDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJ73</accession>
    <name>R5VJ73_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Gp37Gp68 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01315</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000121">
        <property type="protein sequence ID" value="CCZ83546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83546">
        <property type="protein sequence ID" value="CCZ83546"/>
        <property type="gene ID" value="BN566_01315"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011101">
        <property type="entry name" value="DUF5131"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07505">
        <property type="entry name" value="DUF5131"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="237" mass="26847" checksum="39E75133B145AACE" modified="2013-07-24" version="1">MPIWNPWHGCTKYSAGCQNCYVYRIDATHGHNSTTVQKTAAFALPMQRTRGGTLKLQPTGEPVYTCLSSDFFLAAADDWRPDIWAMIRYRSDLQFFIITKRIVRAAACFPPDWGTGYPNVTICCTAENQQAADERLPVLLQLPIAKKEIICEPLLTPIQLQPYLQPQISRVTVGGESGEQARLCRWEWVTSLAAQCRAANVPFVFKQTGARFEKDGICYKIPRRLQQIQAQKAMQFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJD1</accession>
    <name>R5VJD1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">tRNA and rRNA cytosine-C5-methylases</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_00158</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000146">
        <property type="protein sequence ID" value="CCZ83631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00158"/>
    <dbReference type="EnsemblBacteria" id="CCZ83631">
        <property type="protein sequence ID" value="CCZ83631"/>
        <property type="gene ID" value="BN566_00158"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001510">
        <property type="term" value="P:RNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018314">
        <property type="entry name" value="Fmu/NOL1/Nop2p_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031341">
        <property type="entry name" value="Methyltr_RsmF_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB/NOP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="PTHR22807"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17125">
        <property type="entry name" value="Methyltr_RsmF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01153">
        <property type="entry name" value="NOL1_NOP2_SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM_MT_RSMB_NOP" evidence="6">
        <location>
            <begin position="30"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="244"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ83631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="310" mass="33789" checksum="8F3A49B14CED362B" modified="2013-07-24" version="1">MPNLNNVPTLPSYLLERLSACYSQEQTDRICAGYAAQRAVSFRVNTLKATAPAVLEQLQTAGIATTPVAWSETAFFCTDSTTEAQLQALSCYENGEIYLQSLSSMLPPIVLQPQPKQEILDMTAAPGGKTTQMAAMTQNQANIMACELHAIRAEKLKYNVEKQGAKRVTVSITDARRLSPYFSFDRILLDAPCSGSGTLSLHTGAGLQNFSPALVQKTVKAQRALLKKAISLLKKNQEMVYSTCSILPEENEEQVAAILKTGQVELLPIAFSGMETLPLLPSRFAETLCVCPDAQYEGFFVAKLRKKANG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJF2</accession>
    <name>R5VJF2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01392</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000147">
        <property type="protein sequence ID" value="CCZ83656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83656">
        <property type="protein sequence ID" value="CCZ83656"/>
        <property type="gene ID" value="BN566_01392"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="98" mass="11081" checksum="3CE4AA47ADDE652D" modified="2013-07-24" version="1">MKREEVFLRQHAIQVAIMCVEAMHADSTTLNPVQEADHFNIMQPLYRILGELDAEAHDFVDADLQALAKQAQAAEEEPELNLSQLDFLESDVVTDDKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJG0</accession>
    <name>R5VJG0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01402</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000148">
        <property type="protein sequence ID" value="CCZ83666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83666">
        <property type="protein sequence ID" value="CCZ83666"/>
        <property type="gene ID" value="BN566_01402"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="51" mass="5652" checksum="50211430DB82357B" modified="2013-07-24" version="1">MNMSKINATQILPIAMILLDVGAAAVCLWHKDHRRAVYWLAAAVLNVTVTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJN1</accession>
    <name>R5VJN1_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5 6">50S ribosomal protein L20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rplT</name>
        <name evidence="7" type="ORF">BN566_01486</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">Binds directly to 23S ribosomal RNA and is necessary for the in vitro assembly process of the 50S ribosomal subunit. It is not involved in the protein synthesizing functions of that subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the bacterial ribosomal protein bL20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000161">
        <property type="protein sequence ID" value="CCZ83751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01486"/>
    <dbReference type="EnsemblBacteria" id="CCZ83751">
        <property type="protein sequence ID" value="CCZ83751"/>
        <property type="gene ID" value="BN566_01486"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07026">
        <property type="entry name" value="Ribosomal_L20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1900.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00382">
        <property type="entry name" value="Ribosomal_L20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005813">
        <property type="entry name" value="Ribosomal_L20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035566">
        <property type="entry name" value="Ribosomal_protein_L20_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10986">
        <property type="entry name" value="PTHR10986"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00453">
        <property type="entry name" value="Ribosomal_L20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00062">
        <property type="entry name" value="RIBOSOMALL20"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74731">
        <property type="entry name" value="SSF74731"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01032">
        <property type="entry name" value="rplT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00937">
        <property type="entry name" value="RIBOSOMAL_L20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ83751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="117" mass="13154" checksum="52B86FF311D3C5C7" modified="2013-07-24" version="1">MARIKGAMMTRKRRNKTLKLAKGYFGAKSKHFKMAKQAVMKSGNYAYVGRKQKKREFRQLWITRISAACKLNGINYSQFMNGCKKAGITLNRKMLSEIAITDAAGFTAIVEKAKAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJS4</accession>
    <name>R5VJS4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01531</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000164">
        <property type="protein sequence ID" value="CCZ83796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01531"/>
    <dbReference type="EnsemblBacteria" id="CCZ83796">
        <property type="protein sequence ID" value="CCZ83796"/>
        <property type="gene ID" value="BN566_01531"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="181" mass="20672" checksum="921FFFA11AF992AA" modified="2013-07-24" version="1">MRQQPLPYRIALGGTLASFCLLFLFITGVLPMFYLIMPMLCSLLILIMAETTSPYWAFIMYLAVGVLSLFITPNKDASLIFILFFGHYPLLRPYLSRIRPGILRILIKLLIFNLCIGCYFGLVIFLFGMAEFLENLGEFGKYGAYILAGCLNVMFLSYDFSLVTGMEFYRVKVLPKLHLQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>R5VK09</accession>
    <name>R5VK09_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Cell division ATP-binding protein FtsE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ftsE</name>
        <name evidence="8" type="ORF">BN566_01610</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Part of the ABC transporter FtsEX involved in cellular division.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer. Forms a membrane-associated complex with FtsX.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
            <orientation evidence="6">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000169">
        <property type="protein sequence ID" value="CCZ83881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01610"/>
    <dbReference type="EnsemblBacteria" id="CCZ83881">
        <property type="protein sequence ID" value="CCZ83881"/>
        <property type="gene ID" value="BN566_01610"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005286">
        <property type="entry name" value="Cell_div_FtsE_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02673">
        <property type="entry name" value="FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6 8" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="7">
        <location>
            <begin position="2"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="35"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="229" mass="25359" checksum="8C5B662D5B7C4B55" modified="2013-07-24" version="1">MVEMRDVSVVYSGGVDALHNVNLKINDGDFAFVVGPSGAGKSTMIKLILKEINPSSGTVFVNGYHLGKLKQRQVPALRRTIGFVFQDFRLIPSMTVYENIAFVLRVIDAPTSYIKKRVAYVIDLVGLQDKAKNYPNELSGGEMQRVAIARALVNDPQLIIADEPTGNIDPELSYEIVDLLREINECGTTILMVTHEHSLVRYFGGRIITIQQGRIVFDEVVGGNLIHES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>R5VK39</accession>
    <name>R5VK39_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Bacterial cell division membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN566_01635</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000172">
        <property type="protein sequence ID" value="CCZ83906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01635"/>
    <dbReference type="EnsemblBacteria" id="CCZ83906">
        <property type="protein sequence ID" value="CCZ83906"/>
        <property type="gene ID" value="BN566_01635"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013437">
        <property type="entry name" value="FtsW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001182">
        <property type="entry name" value="FtsW/RodA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30474">
        <property type="entry name" value="PTHR30474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01098">
        <property type="entry name" value="FTSW_RODA_SPOVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02614">
        <property type="entry name" value="ftsW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="9" id="KW-0132">Cell division</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0133">Cell shape</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="32"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="125"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="162"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="186"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="301"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="334"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="367"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="398"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="398"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ83906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="441" mass="48356" checksum="FA3A10A8DAE1FB41" modified="2013-07-24" version="1">MAETTVSQAEKKKNRFRFPLWQKGVRYGEVDLAFFLIVIALLVIGIIMMFSASYAWAISEGLEGSYYAKEQIKMAVIGLAAMWFLSIVDYHLYKTPGIAICAYVVPVILLILVLLVGTSEGGAQRWLVIGSFNFQPSELMKIGIVIFFAYHIEKNYDRMNRFKTGVLPYLLALAVVAALLMMEPHLSGTILICTVALSMIIVGGIRPMHFLELAVTGVAAIIGIVLYLSISKGYGYFQTRIQSWLDPFADSQGGTWQTCQSLIAIGSGGLFGLGLGESRQKYLYLPETKNDFVFSIVCEELGFVGALTVVLLFVLLLVRGFYIASHAHDKFGMLLTVGFTVHIGLQAFINIGVVSNLIPNTGISLPFFSYGGTALILQLAEMGIVLNISRARYRAEVPEKEPSAAHTMEQKPEQKAEPKSEPEQHPVHEPTGNGNPAVGTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5VKK4</accession>
    <name>R5VKK4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01742</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000180">
        <property type="protein sequence ID" value="CCZ84016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84016">
        <property type="protein sequence ID" value="CCZ84016"/>
        <property type="gene ID" value="BN566_01742"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="421"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="573" mass="68089" checksum="2DB8478F3BCF0831" modified="2013-07-24" version="1">MDFNNFITNINTSISKLYKKHPGIYQCMVMMVVDPNILENNGKLKCYGHFNMVFNQHILFDSLSKTKNGSPTHMLRILKNPRACTFEKETGYFYNYCEYKYTHNSYSEIDCNYVSSMLYYIVTEQVEIDWSEAAVIWKTFLQKFSDLVGSQYLSISNFLEVSILKDLLLKLEEHAKVSYINPKSYCYEAARIMLCLEAVSVQLTKEEKFIPRTRKYVSYYPMRNKEVLVDYHPMQLTDAEIMRTILDFDEKFNKSESIQKIYRNLDAYCRNMLSLRNKRYRNIYISDDQKRIRKFTDIGSSNEIVAYLTFFETMFLILKNCINSFQLINDLFKYRFILIVNNPYVQEQEIFPFRSTYNNIFNFYKYWKRLEATSPRIEENNEIYMPFNDLAANYVIYKLMNSDNLSTTFQPYSKYYIESEFVIRKCNKSDVKALFSLYNSSADKIIDMVKSEISTAIDHDAVWEIEQYDKAVAVAMIFTNLEHVKNIDPRIKVMDACMIDAIFVEESYRGLEFQNLFLSLANHIAKNKNIFAKVTPDNEPAIRNLRNFGFKKYPLEFGTDKPKYDYYWFQNFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VKN7</accession>
    <name>R5VKN7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">RHS repeat-associated core domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01797</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000186">
        <property type="protein sequence ID" value="CCZ84071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84071">
        <property type="protein sequence ID" value="CCZ84071"/>
        <property type="gene ID" value="BN566_01797"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="150" mass="17344" checksum="0B2B48DC06078D4D" modified="2013-07-24" version="1">MKKEKQHIIDLIYQLDFENAAKHLFQLSKEEQISLMVCEAADTDSIIILGFLSYILSIQNTAFYHQVISEALIQMCWLEGGYNLAFYHACKLLELKPVVEVKEYLLFFNTIPEKLLSEEKAYTLSKEIIAEKPDSIAANGIFSRLRTKYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5VKP4</accession>
    <name>R5VKP4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01807</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000186">
        <property type="protein sequence ID" value="CCZ84081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84081">
        <property type="protein sequence ID" value="CCZ84081"/>
        <property type="gene ID" value="BN566_01807"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026002">
        <property type="entry name" value="ATC_hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14196">
        <property type="entry name" value="ATC_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="209" mass="24124" checksum="C8D20D222562AB2E" modified="2013-07-24" version="1">MKNTLNVKMLYQFYHHAVKAELKRNGFSADIAKKINAEHRKIIERAKEIGKSRLLSSYIMTSYFIAMNRSTGKSAEENYELFRDGLCASKLFHKVIGDVEQYLDPKKMPGRLQWSADSHKRKYENDWVVDILPGTETYDLGYDYHVCGACKLCQDEGCPELAAYICRMDYVLAEIMHMKLVRTGTIAEGAPYCDFRYSRLPAAEHRKES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>R5VLB4</accession>
    <name>R5VLB4_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Chaperonin GroEL</fullName>
            <ecNumber evidence="6">5.6.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">60 kDa chaperonin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Chaperonin-60</fullName>
            <shortName evidence="6">Cpn60</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">groEL</name>
        <name evidence="6" type="synonym">groL</name>
        <name evidence="9" type="ORF">BN566_00249</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 8">Together with its co-chaperonin GroES, plays an essential role in assisting protein folding. The GroEL-GroES system forms a nano-cage that allows encapsulation of the non-native substrate proteins and provides a physical environment optimized to promote and accelerate protein folding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 8">Forms a cylinder of 14 subunits composed of two heptameric rings stacked back-to-back. Interacts with the co-chaperonin GroES.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the chaperonin (HSP60) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.1.7" evidence="6"/>
    <dbReference type="EMBL" id="CBAR010000215">
        <property type="protein sequence ID" value="CCZ84336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00249"/>
    <dbReference type="EnsemblBacteria" id="CCZ84336">
        <property type="protein sequence ID" value="CCZ84336"/>
        <property type="gene ID" value="BN566_00249"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03344">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00600">
        <property type="entry name" value="CH60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018370">
        <property type="entry name" value="Chaperonin_Cpn60_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001844">
        <property type="entry name" value="Cpn60/GroEL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00298">
        <property type="entry name" value="CHAPERONIN60"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="SSF48592"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="SSF52029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="SSF54849"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02348">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00296">
        <property type="entry name" value="CHAPERONINS_CPN60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="29"/>
            <end position="32"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="86"/>
            <end position="90"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="413"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="495"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ84336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="543" mass="57779" checksum="EEBBDEFD0EFE40F3" modified="2013-07-24" version="1">MAKEIKYNADARNAIVAGIDKLADTVKITLGPKGRNVVLDKEFGAPLVTNDGVTIAKDIELEDAFENMGAQLVREVATKTNDAAGDGTTTATVLAQAIIHEGLKNIAAGANPMVLRKGIAKAVDAAVDTIKANSKKVSGSEDIARVATISSADEKVGKLIAEAMEKVTTDGVITIEEGKTAETYSEVVEGMQFDRGYLSPYMITDREKMKVVYDDALVFLTDRTISNIQEILPLLEQIVQQGKKLLIIAEDIEGEALTTIILNNLRGTFKCAAVKAPGFGDRRKEMLQDIAILTGAQVISKDLDMELKDATLEMLGHVSQAVIDKENTILVGGAGKKEDIEDRIQEIKNQIAVSTSDFDKEKMHERIGKLSGGVAVIHVGAATEVEMKEKKLRIEDALSATKAAVEEGIVAGGGTALINAIPAIEKLVPTLKGDEKTGAEIILRALEAPLRQIAANAGLEGSVIIDKIRRSRKVGYGFDAYTETYCDMLSSGIVDPTKVTRSALQNAASIASMVLTTESAVANIKKDEPAAPAMPAGGMGGMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>R5VLJ9</accession>
    <name>R5VLJ9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP synthase subunit alpha</fullName>
            <ecNumber evidence="10">7.1.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">ATP synthase F1 sector subunit alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">F-ATPase subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">atpA</name>
        <name evidence="14" type="ORF">BN566_00328</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Produces ATP from ADP in the presence of a proton gradient across the membrane. The alpha chain is a regulatory subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + 4 H(+)(in) + H2O = ADP + 5 H(+)(out) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:57720"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.2" evidence="10"/>
    <dbReference type="EMBL" id="CBAR010000225">
        <property type="protein sequence ID" value="CCZ84421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00328"/>
    <dbReference type="EnsemblBacteria" id="CCZ84421">
        <property type="protein sequence ID" value="CCZ84421"/>
        <property type="gene ID" value="BN566_00328"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045261">
        <property type="term" value="C:proton-transporting ATP synthase complex, catalytic core F(1)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18113">
        <property type="entry name" value="ATP-synt_F1_alpha_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01132">
        <property type="entry name" value="F1_ATPase_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.150.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01346">
        <property type="entry name" value="ATP_synth_alpha_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023366">
        <property type="entry name" value="ATP_synth_asu-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000793">
        <property type="entry name" value="ATP_synth_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038376">
        <property type="entry name" value="ATP_synth_asu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033732">
        <property type="entry name" value="ATP_synth_F1_a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005294">
        <property type="entry name" value="ATP_synth_F1_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00306">
        <property type="entry name" value="ATP-synt_ab_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="SSF50615"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00962">
        <property type="entry name" value="atpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 10" id="KW-0139">CF(1)</keyword>
    <keyword evidence="10" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="6 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-1278">Translocase</keyword>
    <keyword evidence="2 10" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATP-synt_ab_N" evidence="13">
        <location>
            <begin position="23"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-synt_ab" evidence="11">
        <location>
            <begin position="147"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-synt_ab_C" evidence="12">
        <location>
            <begin position="369"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="167"/>
            <end position="174"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Required for activity" evidence="10">
        <location>
            <position position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CCZ84421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="499" mass="53981" checksum="6075425D9B24BD64" modified="2013-07-24" version="1">MNASEIISVLKSEIADFDRKTGVQEGGTVVRVGDGIATVYGMQHAMYGELVEFETGVRGIIQNLEEKTVGVVLLGSDYGIVEGTQVMRTKKRAGVGVGSGLIGRVVDALGAPIDGGAPIEPEDYYPIEREAAGVADRKSVSVPLQTGILSIDSMFPIGRGQRELIIGDRQTGKTSIAVDTIINQKGQDVICIYVAIGQKSSTVAKIVNTLKAHGAMDYSVVVSASASAPAPVQYIAPYSGTAMAEFFMHQGKDVLIIYDDLSKHAVAYRAMSLLLHRPPGREAYPGDVFYLHSRLLERSCRLDDAHGGGSITALPIIETQAGDISAYIPTNVISITDGQIFLESELFFAGMRPAVNVGLSVSRVGGAAQTKAMKKVAGKMRMELAQFREMEVFTQFSSDLDPATQAMLDHGHALMELLKQPLYHPQPLWRQVCVLAVASERLFDDIPLKNLRDFVNDLMAHLEQSQAALIKKIQETGQLSDEDKATLIAEAEAYKKQVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5VLM8</accession>
    <name>R5VLM8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Prephenate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00050</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000230">
        <property type="protein sequence ID" value="CCZ84451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00050"/>
    <dbReference type="EnsemblBacteria" id="CCZ84451">
        <property type="protein sequence ID" value="CCZ84451"/>
        <property type="gene ID" value="BN566_00050"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00056">
        <property type="reaction ID" value="UER00094"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008977">
        <property type="term" value="F:prephenate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004665">
        <property type="term" value="F:prephenate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006571">
        <property type="term" value="P:tyrosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003099">
        <property type="entry name" value="Prephen_DH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02153">
        <property type="entry name" value="PDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51176">
        <property type="entry name" value="PDH_ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prephenate/arogenate dehydrogenase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="272"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="303" mass="33743" checksum="D8B9834D7B445CB8" modified="2013-07-24" version="1">MKILIVGMGLIGGSVCKALKNKTQYEVYGCDVDESVLQQALQDHAIDGTGKMEDGIYDVCIVCLHHRIAQPCIKQALPFLKKGSILMDMCGLKGQMVLEFTNACRMAGLYYVGTHPMAGREKSGYYASIPNLFDGANFIITPTYGTSNEAVERVSALAKEIGFGKLVLTTPHRHDDMIAYTSQLAHIVSSAYVKDDCMDDAVSFSGGSFQDMTRVATMDEKMWASLFMDNRLLLLHHLELLIAHLSEYRTALELQDESALQLLIADGRRVQEENVRKRKRALEERAQLLNEAAKEETKKEQNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5VLZ9</accession>
    <name>R5VLZ9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">50S ribosomal protein L17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rplQ</name>
        <name evidence="6" type="ORF">BN566_00087</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Part of the 50S ribosomal subunit. Contacts protein L32.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the bacterial ribosomal protein bL17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000241">
        <property type="protein sequence ID" value="CCZ84541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00087"/>
    <dbReference type="EnsemblBacteria" id="CCZ84541">
        <property type="protein sequence ID" value="CCZ84541"/>
        <property type="gene ID" value="BN566_00087"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1030.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01368">
        <property type="entry name" value="Ribosomal_L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000456">
        <property type="entry name" value="Ribosomal_L17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036373">
        <property type="entry name" value="Ribosomal_L17_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14413">
        <property type="entry name" value="PTHR14413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01196">
        <property type="entry name" value="Ribosomal_L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64263">
        <property type="entry name" value="SSF64263"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00059">
        <property type="entry name" value="L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01167">
        <property type="entry name" value="RIBOSOMAL_L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ84541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="113" mass="12676" checksum="BD2444DB4EB5F8BF" modified="2013-07-24" version="1">MPGSRKLGRTTDHRKAMLRAMVTFLLENGQIETTVTRAKEVRAVAEKMITIAKTNDLHSKRQVMAYVTKESVAKKLFDEIAPNYADRQGGYTRIIKTGPRRGDAAEMALIQLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5VM18</accession>
    <name>R5VM18_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00432</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000247">
        <property type="protein sequence ID" value="CCZ84571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00432"/>
    <dbReference type="EnsemblBacteria" id="CCZ84571">
        <property type="protein sequence ID" value="CCZ84571"/>
        <property type="gene ID" value="BN566_00432"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="2">
        <location>
            <begin position="18"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="198"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="373" mass="41201" checksum="EF93B15F9D07E21D" modified="2013-07-24" version="1">MSRPLRILLVNKAYPPHIGGIETLVRQYARCFQTQFSAEVQVLVCQEKGKTARETIEGVPVLRAGSIGTYFSCPLSGSFLRYFRKYAKQADVVMIHMPFPLADLACLLSGYRGRVVLAWHSDVIKQKTLLRFYAPLLRWLLRRADCILTATQGHIDSSAFLPAVREKCKVIPYGIDLAAYRAAPAYPVLQPHSNHPKAVKALFVGRLVYYKGVTILLKAMQQVQNCELFLIGSGKLQDTLQQMAEGMDTVHFLGSLSDADLRAALQECDFLVLPSVANSEAFGLVQLEAMVYGKPVINTALPTGVPHVSLDGETGLTVPPESETALAKAMQTLVSDADLRRRYGAAAKKRVETVFAEDVVMQQVYDVLKDSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VM32</accession>
    <name>R5VM32_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00452</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000250">
        <property type="protein sequence ID" value="CCZ84591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84591">
        <property type="protein sequence ID" value="CCZ84591"/>
        <property type="gene ID" value="BN566_00452"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="187" mass="22510" checksum="7936DB3BE2653DF0" modified="2013-07-24" version="1">MNSKYNWNEIVKDSKKIIHDTTKNGNRKLISTMKELYDRLKFQYEKKGKEVPRYVLYSEKIRKEFNIDCHKKNIQLELYRAVAAHGKILLKTLSQNITISYPNVASQAVWLFIRLKETKETEEKNISLEDYNSHFYYLSKRLKEQFKDEILFLSFDTDTVVLLCKDDSARKKIIRHFRKLHPHLEMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5VQG1</accession>
    <name>R5VQG1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PTS HPr component phosphorylation site</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00632</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000011">
        <property type="protein sequence ID" value="CCZ82827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00632"/>
    <dbReference type="EnsemblBacteria" id="CCZ82827">
        <property type="protein sequence ID" value="CCZ82827"/>
        <property type="gene ID" value="BN566_00632"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1340.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000032">
        <property type="entry name" value="HPr-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035895">
        <property type="entry name" value="HPr-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00381">
        <property type="entry name" value="PTS-HPr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55594">
        <property type="entry name" value="SSF55594"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51350">
        <property type="entry name" value="PTS_HPR_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HPr" evidence="1">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="76" mass="8567" checksum="6ACFF93EF694A845" modified="2013-07-24" version="1">MYETTISLQAINDVKDFVNTVMLFNYDIDLVSGRYAVDAKSIMGIFSLDLSKPIKLQAHTEDPEALIKAIDKFIVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5VQI0</accession>
    <name>R5VQI0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00647</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000012">
        <property type="protein sequence ID" value="CCZ82842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82842">
        <property type="protein sequence ID" value="CCZ82842"/>
        <property type="gene ID" value="BN566_00647"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028956">
        <property type="entry name" value="Imm51"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15595">
        <property type="entry name" value="Imm51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ82842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="120" mass="13903" checksum="16B035FD9B8946F9" modified="2013-07-24" version="1">MQDLVTVLSNEDGIQISLDVQQDAVLQIGEMMQEIQEEAYMNGENWAVFFQYYLGKYAPNLLEGLESDSEAGMYVAYYDKVTPENHRKAMQLADLFNALLSHPEQIYQILRTTEDEIEWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>R5VQX8</accession>
    <name>R5VQX8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcriptional regulator LacI family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_00757</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000032">
        <property type="protein sequence ID" value="CCZ82957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00757"/>
    <dbReference type="EnsemblBacteria" id="CCZ82957">
        <property type="protein sequence ID" value="CCZ82957"/>
        <property type="gene ID" value="BN566_00757"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01392">
        <property type="entry name" value="HTH_LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000843">
        <property type="entry name" value="HTH_LacI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046335">
        <property type="entry name" value="LacI/GalR-like_sensor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00356">
        <property type="entry name" value="LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13377">
        <property type="entry name" value="Peripla_BP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00354">
        <property type="entry name" value="HTH_LACI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50932">
        <property type="entry name" value="HTH_LACI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="16"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="HTH lacI-type" evidence="5">
        <location>
            <begin position="50"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ82957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="279" mass="31783" checksum="F7A3D396B9A7C48C" modified="2013-07-24" version="1">MFFDLSDYFNGIYCDFYLTTMSFLLTIRIAVVIIKHIIQIDFIGYWVKNMSITKIAAITGASVSTVARVLRDSEHKCHSMELKEKILQTAREIGYVPNEAARALKSRKIALTAVNYLISCGHQKIGYVEDCHIGYQEALIHHGIAPDIDDVYDIMPNEKNGYHAMAYFSKLETPPTAFYCENDILAIGMLKYLAQSKKWYYHPSIISSDNIVESQYTTPMLTTVSLPKAEMAHLAIQILTDRMDGGHKAIVKTELQSTLIIRQSVRQYISTENEPEYYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5VRF6</accession>
    <name>R5VRF6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phenazine biosynthesis protein PhzF family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00929</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000063">
        <property type="protein sequence ID" value="CCZ83142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00929"/>
    <dbReference type="EnsemblBacteria" id="CCZ83142">
        <property type="protein sequence ID" value="CCZ83142"/>
        <property type="gene ID" value="BN566_00929"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003719">
        <property type="entry name" value="Phenazine_PhzF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13774">
        <property type="entry name" value="PTHR13774"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02567">
        <property type="entry name" value="PhzC-PhzF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016184">
        <property type="entry name" value="PhzC_PhzF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00654">
        <property type="entry name" value="PhzF_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="1">
        <location>
            <position position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR016184-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="264" mass="29491" checksum="1E2D3A193014B673" modified="2013-07-24" version="1">MKQYIVDAFTDRIFSGNPAAVCVLEHWLPEQQMQNLAMENNLSETAFIVPEQDGYQLRWFTPSGEIDFCGHATLAAAYVLFRFVQQQATTLFFSTQSGNITIQKQADCYEMNFPAYTCHPVAVTAEMEQAIGVKPLEAYLDRDLLLVLENADAVRNLQPDFAKLSQLDGVCIAVTARDTEYDCISRVFAPKMGVPEDPVTGSTHCMIVPYWCEKLRKSSLTALQASRRSGVLYAKQCGNRIQIAGKAVLFAVSEIFMETEAETK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>R5VRH7</accession>
    <name>R5VRH7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Aconitate hydratase A</fullName>
            <ecNumber evidence="3">4.2.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Iron-responsive protein-like</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">RNA-binding protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">BN566_00945</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>citrate = D-threo-isocitrate</text>
            <dbReference type="Rhea" id="RHEA:10336"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16947"/>
            <dbReference type="EC" id="4.2.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 2/2.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.3" evidence="3"/>
    <dbReference type="EMBL" id="CBAR010000069">
        <property type="protein sequence ID" value="CCZ83162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00945"/>
    <dbReference type="EnsemblBacteria" id="CCZ83162">
        <property type="protein sequence ID" value="CCZ83162"/>
        <property type="gene ID" value="BN566_00945"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00718"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003994">
        <property type="term" value="F:aconitate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047780">
        <property type="term" value="F:citrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006250">
        <property type="entry name" value="Aconitase_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="SSF53732"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01342">
        <property type="entry name" value="acon_putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Aconitase" evidence="12">
        <location>
            <begin position="7"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase_C" evidence="13">
        <location>
            <begin position="508"/>
            <end position="577"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CCZ83162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="646" mass="69035" checksum="269FE8050B692E20" modified="2013-07-24" version="1">MGLTLTEKILKAHLVEGEMKKGTEIGIRIDQTLTQDATGTMAYLEFEAMGVPRVRTERSVAYIDHNTLQSGFENADDHRFIGSVAKKHGIYFSRPGNGICHQVHLERFGVPGKTLIGSDSHTPTGGGIGMIAMGAGGLDVAVAMGGGTYYITCPKIVKVELTGKLSPWVAAKDVILEVLKRLSVKGGVGKVIEYCGEGVKTLSVPERATITNMGAELGATTSIFPSDEVTKQFLEAQGRGEVWTEQKADPDAVYDEVVTINLSELVPLAACPHSPDNVKSVAEIGKLKIDQVCIGSCTNSSLLDMMKVAYILKGKTVNPDVSLSIAPGSKQVLTMMAELGLLEIMIDAGARILESACGPCIGMGQSPNSGGISLRTFNRNFLGRSGTKDGQIYLVSPELAAYSALTGYLSDPRELGEMPTFTLPEKFKVHDNMIVKPVPAEEMDSVEILRGPNIKPFPETAPLEATISAGCSLKVGDNITTDHIMPAGAKILPLRSNIPAISQHCFTVCDEAFPTRAKEMGKSIIVGGSNYGQGSSREHAALAPLYLGVKAVLVKSFARIHRANLINAGILPLTFVNEADYEKIAQGDELELADVRKHIENGETTLTLVNKTTGVEIPVLCELTGRTKDIILAGGLLDYTRDALSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>R5VRI2</accession>
    <name>R5VRI2_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Redox-sensing transcriptional repressor Rex</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rex</name>
        <name evidence="9" type="ORF">BN566_00950</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Modulates transcription in response to changes in cellular NADH/NAD(+) redox state.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the transcriptional regulatory Rex family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000069">
        <property type="protein sequence ID" value="CCZ83167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00950"/>
    <dbReference type="EnsemblBacteria" id="CCZ83167">
        <property type="protein sequence ID" value="CCZ83167"/>
        <property type="gene ID" value="BN566_00950"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051775">
        <property type="term" value="P:response to redox state"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01131">
        <property type="entry name" value="Rex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009718">
        <property type="entry name" value="Rex_DNA-bd_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022876">
        <property type="entry name" value="Tscrpt_rep_Rex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35786">
        <property type="entry name" value="PTHR35786"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02629">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06971">
        <property type="entry name" value="Put_DNA-bind_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 7" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0678">Repressor</keyword>
    <keyword evidence="6 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CoA_binding" evidence="8">
        <location>
            <begin position="81"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="7">
        <location>
            <begin position="17"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="91"/>
            <end position="96"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ83167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="214" mass="23412" checksum="F1E48C58B9D4D3E6" modified="2013-07-24" version="1">MMLKEPVSDSVVRRLPRYYRYLSELERAGVERTSSQALATQLGSTASQIRQDLNCFGGFGHQGYGYHVSDLRTQLGRIIGLEQCMPAVLIGAGHLGSVIAVNIDFPAYGFYLTGIFDIVPTVCGQVLGGLTVQHMTQLSDFCEQVHPAAAVLCIPEHAAQAVADDLVHLGVHGFWNFSHCDLEVDRNTIQVENVHLGDSLMALSYRLHQDGGNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>R5VSE2</accession>
    <name>R5VSE2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">GHMP kinase N-terminal domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_01221</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000105">
        <property type="protein sequence ID" value="CCZ83447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01221"/>
    <dbReference type="EnsemblBacteria" id="CCZ83447">
        <property type="protein sequence ID" value="CCZ83447"/>
        <property type="gene ID" value="BN566_01221"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004335">
        <property type="term" value="F:galactokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006012">
        <property type="term" value="P:galactose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000705">
        <property type="entry name" value="Galactokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019539">
        <property type="entry name" value="GalKase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006206">
        <property type="entry name" value="Mevalonate/galactokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10457:SF6">
        <property type="entry name" value="PTHR10457:SF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10509">
        <property type="entry name" value="GalKase_gal_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000530">
        <property type="entry name" value="Galactokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00473">
        <property type="entry name" value="GALCTOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="SSF55060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GalKase_gal_bdg" evidence="6">
        <location>
            <begin position="42"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP_kinases_N" evidence="5">
        <location>
            <begin position="150"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF10509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ83447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="428" mass="46959" checksum="848FF4200AA1F4D3" modified="2013-07-24" version="1">MKATEWTEAFASGKMDDKLSLLYGNAKATLEQQRQRYVDAIQAFTELYPEREDLYIYSAPGRTEVGGNHTDHQHGCVLAAAVNLDAIAIVSFHEDQVIRLKSANYPQDVVDLTDLSVHLAEMGKSAALIRGIAARFAEMGVSVSGFDAYTTSSVLSGSGLSSSAAFEVLVGTIIDQYYNEGKAGAVEIAKIGQYAENRYFGKGSGLMDQMVSSVGGFVYIDFADVENPKIQQHNLDFEHAGYHICITDTKGSHADLTEDYVSIPQEMKQVAEQFGKQYLREVEPAEFFDAIPKLHGVCSDRAIMRAVHFFDENRRALLENNALAADNLDFFFDLVRQSGDSSANLLQNLYSCRKPTEQGIPFGIMMSKRFLGKSGAVRVHGGGFAGTIQAFVPTKVTEDYVKEMEALFGAGSCYVLRIRPVGGFSFQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5VSS1</accession>
    <name>R5VSS1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Potassium uptake protein KtrB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_01345</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000132">
        <property type="protein sequence ID" value="CCZ83577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01345"/>
    <dbReference type="EnsemblBacteria" id="CCZ83577">
        <property type="protein sequence ID" value="CCZ83577"/>
        <property type="gene ID" value="BN566_01345"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003445">
        <property type="entry name" value="Cat_transpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02386">
        <property type="entry name" value="TrkH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="45"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="81"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="110"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="256"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="284"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ83577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="351" mass="37923" checksum="798754FBDCC13651" modified="2013-07-24" version="1">MQIGGLGITSIGAGLIAFTGRKLRMRDQVLVQEALNYPTMKDVQVLIRSVLITTIIVESSAAFVGWFLFRRQFDVLPALGIAIFHSVSTFNHAGFDILGGLLSYRNDVGLQLLTIAENILGGFGFFVILDILRNQRFHKFSLHTKVVLITSGFLDLTSSLLLKAIEGDSLSWVNAFFMGITSRTSGLTIANVGDFSNASQLLICVLMYIGASPGSTGGGVKVTTIFVLFRTIVAYPRGEQPGAFRKRFSAGTVQQAMVICSLAISIVFAISFILCLLNPTLPFLSLLFETASAFSTVGLSTGITSSVATFGKWLLMAAMFGGRLGTLTVVSLWFRKHRQTILLPVESISVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5VST4</accession>
    <name>R5VST4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">N-acetylmuramoyl-L-alanine amidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00137</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000135">
        <property type="protein sequence ID" value="CCZ83592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00137"/>
    <dbReference type="EnsemblBacteria" id="CCZ83592">
        <property type="protein sequence ID" value="CCZ83592"/>
        <property type="gene ID" value="BN566_00137"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008745">
        <property type="term" value="F:N-acetylmuramoyl-L-alanine amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009253">
        <property type="term" value="P:peptidoglycan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06583">
        <property type="entry name" value="PGRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036505">
        <property type="entry name" value="Amidase/PGRP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002502">
        <property type="entry name" value="Amidase_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01510">
        <property type="entry name" value="Amidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00644">
        <property type="entry name" value="Ami_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55846">
        <property type="entry name" value="SSF55846"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetylmuramoyl-L-alanine amidase" evidence="1">
        <location>
            <begin position="32"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="221" mass="24764" checksum="8A2B37E5FA4C79C6" modified="2013-07-24" version="1">MAILRPDTTTTLNRVKINEYLLTRHNPNRIAMPSVSMEGKIIGVTVHNTAWITTAAGTTPAEQYTRATVNGNMNDVRVHYYVDNTCAWQNLPHSLSGWHAADGSGNGNRRTIAIECIMSSAYNATDKKSEDNCARLAAALLKKYNLDINHLFTHTHWLNVRDGKSGSVDYLNTARNFYKMCPLYILPHWSDFKKKVQGYMNAGSATTSTPSLHLRQNSFTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VSZ4</accession>
    <name>R5VSZ4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01393</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000147">
        <property type="protein sequence ID" value="CCZ83657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83657">
        <property type="protein sequence ID" value="CCZ83657"/>
        <property type="gene ID" value="BN566_01393"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="81" mass="8850" checksum="F61F7523D0135711" modified="2013-07-24" version="1">MIKIENDCSCCERCGNCGAKRVPHVYCDGCGAEVSGDSKLIRIVGNDDAWLCEDCLAEWVESVTVTTYAADLVREMNAEDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5VT34</accession>
    <name>R5VT34_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 3">Hypoxanthine phosphoribosyltransferase</fullName>
            <ecNumber evidence="1 3">2.4.2.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_01433</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>diphosphate + IMP = 5-phospho-alpha-D-ribose 1-diphosphate + hypoxanthine</text>
            <dbReference type="Rhea" id="RHEA:17973"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="2.4.2.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Purine metabolism; IMP biosynthesis via salvage pathway; IMP from hypoxanthine: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.8" evidence="1 3"/>
    <dbReference type="EMBL" id="CBAR010000153">
        <property type="protein sequence ID" value="CCZ83697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01433"/>
    <dbReference type="EnsemblBacteria" id="CCZ83697">
        <property type="protein sequence ID" value="CCZ83697"/>
        <property type="gene ID" value="BN566_01433"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00591">
        <property type="reaction ID" value="UER00648"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052657">
        <property type="term" value="F:guanine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004422">
        <property type="term" value="F:hypoxanthine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032264">
        <property type="term" value="P:IMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005904">
        <property type="entry name" value="Hxn_phspho_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01203">
        <property type="entry name" value="HGPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3 5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pribosyltran" evidence="4">
        <location>
            <begin position="22"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU364099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="184" mass="20629" checksum="14B866D688268278" modified="2013-07-24" version="1">MNSETFRKNIKQILIPETDLQAKIKEVGAQISKEYDGKPLLLVSILNGAFVFMADLCRAITIPCETAFMCAKSYYSGTVSTGVVKITMDLQQDISNYHVIIAEDIIDTGRTLNDVKKMLLARNPLSLKIITLLDKPDRRLVDLKADLSLFTIPDLFVIGYGLDCGELYRNLPYIAEYDEEGQAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5VT98</accession>
    <name>R5VT98_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01502</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000161">
        <property type="protein sequence ID" value="CCZ83767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83767">
        <property type="protein sequence ID" value="CCZ83767"/>
        <property type="gene ID" value="BN566_01502"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="107" mass="11641" checksum="D28A6C819E250268" modified="2013-07-24" version="1">MENGKKMITVVGAYMILKGILNLILGFSASNLVSLLFAAVLIFAMLKRIPYSQYVTAIFLALIFLVNLPANLSNLGSNWIYLVEGLLDVGAAAILAFHKDVKDYFAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5VTS5</accession>
    <name>R5VTS5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Acetyltransferase GNAT family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01651</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000173">
        <property type="protein sequence ID" value="CCZ83922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01651"/>
    <dbReference type="EnsemblBacteria" id="CCZ83922">
        <property type="protein sequence ID" value="CCZ83922"/>
        <property type="gene ID" value="BN566_01651"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004343">
        <property type="term" value="F:glucosamine 6-phosphate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039143">
        <property type="entry name" value="GNPNAT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13355">
        <property type="entry name" value="PTHR13355"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="6"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="138" mass="16031" checksum="4AF5D7F63FBC689E" modified="2013-07-24" version="1">MLNLKIKYNELTAEQFIELWETVWGNGPSLEQTRLAMKHTLFRVSIWDGDCIVAMARMNGDMGLNYYIKDVVVRPEYQGKGIGRMLINELRKFINNNGVKGTDIFVELCAVPDKIPFYEKFGFEANEAQRLKIFHHVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5VTU7</accession>
    <name>R5VTU7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyl transferase family 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01671</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000174">
        <property type="protein sequence ID" value="CCZ83942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01671"/>
    <dbReference type="EnsemblBacteria" id="CCZ83942">
        <property type="protein sequence ID" value="CCZ83942"/>
        <property type="gene ID" value="BN566_01671"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040492">
        <property type="entry name" value="GlfT2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17994">
        <property type="entry name" value="Glft2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glft2_N" evidence="1">
        <location>
            <begin position="29"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="561" mass="64453" checksum="F1D8785DA8B6DACE" modified="2013-07-24" version="1">MNIELYELCLPKSESAPTALYIRTEGTVTRTANHLQLACESAVSFDTWFGCFDYLQWRRYTTLTTPIAAFFIKGTMLVQCYVRTAKRTSLLYEQTITSDGSQPQVFPFSISNLPENGFLYFRLQAKSENCLFLRGCYLADVSELHAIQVAAVICTYQRESWVSNTLQQFLQWQKEHHTLPVDIFLIDNGQTWTEEQLPDSNQIHLIPNQNTGGSGGFTRGIQEALHREKYTHVLLMDDDIIADWNGVQRAISFLSICKPEQRKTLTIGGTMMDITQPAMQFEAGACCQNRCLTGIQPQADLTTLSSLQARMQPIPANYAAWWMCLLPVAAMKQAGLPMPFFIKMDDVEYALQIKEQVVMMTGVCVWHENFHLKYAPWNEYYITRNGCITYALHGQAKNAVSVVLELWRTMLHLLFLHRYDTAKFVLQGYRDFLRGWKWLQKTDAITLHRQILQQQQAATEQFQTDCTEVPNSIPQVSFFEATRAAVQKQKSVFLFHPFTKKGFYASRKKGTAFQLLLKMGGISLRMLTTYRSVVQGFQKNRTILCEDAAWNRRNQHNSEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5VTV7</accession>
    <name>R5VTV7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_01681</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000175">
        <property type="protein sequence ID" value="CCZ83952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01681"/>
    <dbReference type="EnsemblBacteria" id="CCZ83952">
        <property type="protein sequence ID" value="CCZ83952"/>
        <property type="gene ID" value="BN566_01681"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="195"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="249"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ83952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="324" mass="36141" checksum="72BAC43FF0E7772D" modified="2013-07-24" version="1">MQEGMVKRASELLADGTVVRVLGWKNGEFAYDPTPAVFESAEALSNFVYNDFCASNLSKYLVALSKKDGKTLVFLKPCDSYSFNQLLTEHRVDREKVYIIGIPCDGKVDESKLRAKGAEGVTGITTDGDQITVSTFYGDLQFQKADVIAERCVTCKSKKVVAYDEMMGENGEVNPDCGRFDQVAELEAMTPDERFAFWRNELSKCIRCNACRNICPACTCEQCVFDNPKSGISQKAAADSFEENMFHIIRAFHVAGRCTDCGECSRVCPQHIPLHLLNRKFIKEMNECYGEYQAGEEAGSRAPLVNFTKEDCEPSIVYTKEASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5VTW1</accession>
    <name>R5VTW1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN566_01686</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the alanine or glycine:cation symporter (AGCS) (TC 2.A.25) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000176">
        <property type="protein sequence ID" value="CCZ83957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01686"/>
    <dbReference type="EnsemblBacteria" id="CCZ83957">
        <property type="protein sequence ID" value="CCZ83957"/>
        <property type="gene ID" value="BN566_01686"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015655">
        <property type="term" value="F:alanine:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001463">
        <property type="entry name" value="Na/Ala_symport"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30330">
        <property type="entry name" value="PTHR30330"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01235">
        <property type="entry name" value="Na_Ala_symp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00175">
        <property type="entry name" value="NAALASMPORT"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00835">
        <property type="entry name" value="agcS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0769">Symport</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="14"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="61"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="94"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="150"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="228"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="262"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="316"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="368"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="412"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="436"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ83957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="470" mass="50204" checksum="02AD2A8F7417BAAA" modified="2013-07-24" version="1">MDSLNNIIKSIDDFVWGIPLIVLIMACGIWLTIRCCGLQFRHLGKALKFMVKNEEGGDGEVSSFAALCTALSATIGTGNIVGVATALAAGGPGALFWMIAAALFGMATKYAEGFLAIKYRTIDAEGHYHGGPYYYIEHGMGAKWKWLAKLFAIFGMLAGLLGIGTITQINGITSAANNFFDADSSHIAFTMFGHDYTWTTVICGLLITIFVALVVIGGLKRVSMVSQVVVPFMAILYVLCVLVIIVTNITKLPSAIVEIVEGAFGIRAAAGGALGAMMVAMQKGVARGIFSNEAGLGSAPIAAAAAQTKEPVRQGLVTMTGTFIDTIVICTMTGLSIVISGEWSNPDLQGVQITTAAFQSGLPIGGKFPAFILMICLIFFAFTTILGWSYYSERCLSYLIGSRKSTTVLYRWLYILAVFIGPYLTVEAVWNMADIFNGLMALPNIIALLALSGVIARDTKAYFKKSALQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5VTZ5</accession>
    <name>R5VTZ5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CDP-diglyceride synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01721</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000178">
        <property type="protein sequence ID" value="CCZ83992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01721"/>
    <dbReference type="EnsemblBacteria" id="CCZ83992">
        <property type="protein sequence ID" value="CCZ83992"/>
        <property type="gene ID" value="BN566_01721"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="174"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="272" mass="29974" checksum="320000C5BA81FAF2" modified="2013-07-24" version="1">MKVRIISGAVAVVLLVVVLLLHKTIVFPLCIAAICAIAIFELFRAKGCLRYRISFYAALLYTISAPILQFFHLEQITEVVMIAAIMILFLDGVVQHKRLKMDQVCFMMVSMLFVTRALGLLQVLLQSDAQFGLIYVILALCAAWIADTGAYFTGTFLGKHKLCPEVSPKKTIEGFLGGIASTIVVFFVFSLIYGSVAEVKVRYVWVIVIAAACAVISVLGDLTASILKRQCSMKDFGNIMPGHGGVMDRFDSALFTVPVFYVLNTLLSIYHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>R5VU49</accession>
    <name>R5VU49_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glutamate-1-semialdehyde 2,1-aminomutase</fullName>
            <shortName evidence="7">GSA</shortName>
            <ecNumber evidence="7">5.4.3.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Glutamate-1-semialdehyde aminotransferase</fullName>
            <shortName evidence="7">GSA-AT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">hemL</name>
        <name evidence="9" type="ORF">BN566_01758</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(S)-4-amino-5-oxopentanoate = 5-aminolevulinate</text>
            <dbReference type="Rhea" id="RHEA:14265"/>
            <dbReference type="ChEBI" id="CHEBI:57501"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="5.4.3.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.3.8" evidence="7"/>
    <dbReference type="EMBL" id="CBAR010000180">
        <property type="protein sequence ID" value="CCZ84032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01758"/>
    <dbReference type="EnsemblBacteria" id="CCZ84032">
        <property type="protein sequence ID" value="CCZ84032"/>
        <property type="gene ID" value="BN566_01758"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00317"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042286">
        <property type="term" value="F:glutamate-1-semialdehyde 2,1-aminomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00375">
        <property type="entry name" value="HemL_aminotrans_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004639">
        <property type="entry name" value="4pyrrol_synth_GluAld_NH2Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00713">
        <property type="entry name" value="hemL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6 7" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="4 7 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="7">
        <location>
            <position position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ84032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="431" mass="47102" checksum="92CE435C088C3431" modified="2013-07-24" version="1">MQTTQSEAWFQRAKEKMPGGVNSPVRAFPSVGGAPRFICKAKGNHLYDLDGNEYLDYIGSWGPMILGHNPDFVLQAVQEQMQYGLSYGTATALEVEMAELISEIVPCVEMIRMVNSGTEAVMSAVRAARGFTGRDKIIKFAGCYHGHADAMLVKAGSGVMTAGIPDSSGVPSGCTKDTLTAVYNDAESVKALLEQYPNEVACVIVEPVAANMGVVPPKEGFLQQLRTLCNQYGALLIFDEVITGFRLQLDGAVGFYHVQPDLVTYGKIIGAGMPVGAYGGRKDIMQLVAPLGSVYQAGTLSGNPVAMRAGLAQLRYLKQHPEVYPYLTERAEQLRNGLKQLIDRYHMPCTVTGVGSLSCLYFTKQTVTNYELAKTADTELFRQYFHFMLERGNYFAPSQFEAIFLSAAHTEADIAKTLSDAESFFQMISHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>R5VUR8</accession>
    <name>R5VUR8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Glycosyl hydrolase family 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN566_00196</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000204">
        <property type="protein sequence ID" value="CCZ84267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00196"/>
    <dbReference type="EnsemblBacteria" id="CCZ84267">
        <property type="protein sequence ID" value="CCZ84267"/>
        <property type="gene ID" value="BN566_00196"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002105">
        <property type="entry name" value="Dockerin_1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001701">
        <property type="entry name" value="Glyco_hydro_9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00759">
        <property type="entry name" value="Glyco_hydro_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="SSF48208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00448">
        <property type="entry name" value="CLOS_CELLULOSOME_RPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004407391" evidence="7">
        <location>
            <begin position="36"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="8">
        <location>
            <begin position="581"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="484"/>
            <end position="581"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ84267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="656" mass="71576" checksum="B572703BFA312934" modified="2013-07-24" version="1">MKKQGKKNRIFAGIAAAVMAVSATAVGVRSYSANAADLELDNYAKLLQYSLYFYDANMCGDKVGETSAFSWRDNCHTGDVALGGFHDAGDAIKCGQTAGFTASTLGWAYYEYKDQFQKTGTTAHFKVISDEFNEFFKRCTTLSGDTVTSFIYEVGDDGSDHGKWFAPEAMWDHGSSETYSTTNGASDIAAQYAASLAQSYLNFGNEEDLKYAVALYNFADKYRTITYDQNTYGGAAKDVQDDISWAAGWLYLATGEESYLTENSKYTQVKNDWTQDYCYANSQLGAAIINAEITGNWSFATNYIGQKVNANQNQFYVMNSWGSARYDVLMQYCALVTSKYEQSGVDYTEWAKKQMNYILGDNNANVCLVVGYNDVSATSPHHRAASNLKVSDDWHEWNSWDGKYSSSNGHVLYGALVGGPTSTDFTTYKDNATDATSNEVAIDYQVGLVAAAAGLYDKYQTGSVVAQIGDEVTVYPDEVAVANGGEVTTTTTTETTTTTTETTTTTEETTTTTEEITTTPEETTTTPEETTTTTTEETTTTPEETTTTTTEETTVTEETTTVSEETTTEETTTTTSASVLPGKNVGDVNLDGKVDLLDAIHLNKYLAQLITLTPVAVANANCNQEDGTETIDDKDTSVLMKFILGSEDVKNLPYVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVF1</accession>
    <name>R5VVF1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Phosphate ABC transporter ATP-binding protein PhoT family (TC 3.A.1.7.1)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_00376</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000238">
        <property type="protein sequence ID" value="CCZ84477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00376"/>
    <dbReference type="EnsemblBacteria" id="CCZ84477">
        <property type="protein sequence ID" value="CCZ84477"/>
        <property type="gene ID" value="BN566_00376"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015415">
        <property type="term" value="F:ATPase-coupled phosphate ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:inorganic phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03260">
        <property type="entry name" value="ABC_PstB_phosphate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015850">
        <property type="entry name" value="ABC_transpr_PstB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005670">
        <property type="entry name" value="Phosp_transpt1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43423">
        <property type="entry name" value="PTHR43423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43423:SF3">
        <property type="entry name" value="PTHR43423:SF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00972">
        <property type="entry name" value="3a0107s01c2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="5"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="37"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ84477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="250" mass="28087" checksum="619D4958FDDC7885" modified="2013-07-24" version="1">MENKFTIKDMNLYYGSFHALKGVNLNLPANEVTAFIGPSGCGKSTLLRSLNRMNDLVEGCKITGDIRLDNEDIYGSMDINLLRKRVGMVFQKANPFPMSIYDNIAYGPRTHGIRSKSKLNEIVEKSLRDAAIWDEVKDRLNKSALGMSGGQQQRLCIARALAVQPEVLLMDEPTSALDPISTSKIEDLVIELKKEYTVIIVTHNMQQATRVSDKTAFFLLGEVVEFNETEKLFSNPNDKRTEDYITGRFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVU6</accession>
    <name>R5VVU6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BNR/Asp-box repeat</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00500</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000265">
        <property type="protein sequence ID" value="CCZ84647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00500"/>
    <dbReference type="EnsemblBacteria" id="CCZ84647">
        <property type="protein sequence ID" value="CCZ84647"/>
        <property type="gene ID" value="BN566_00500"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002105">
        <property type="entry name" value="Dockerin_1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00448">
        <property type="entry name" value="CLOS_CELLULOSOME_RPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004395719" evidence="1">
        <location>
            <begin position="39"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="2">
        <location>
            <begin position="861"/>
            <end position="934"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="938" mass="100708" checksum="391B1F361DCB4967" modified="2013-07-24" version="1">MKKAMKRLLAATSAGAVLCGSLALSSLLGSSTTLTASAATDSVADQTKVGTLGIAGGGFVSGIITGKNVMYARTDVGGAYKYNYDTGNWDQMLDDLNDSERGFLSVDAMCIDPTDDDTIYLLCGCAYFSDAKTEIFRSRDGGKTFDRIDVTDLIQVHGNGEGRQFGEAIAVDPDNPNIIYCGGDVASGDSALIMSKDGGDTWEAVKGYDDLGLFKNSIKWPTWTEHMARALTDAEYYHQNGVSIVHVQDGKVYVGTTIQGENSLVVADVGSDDFQPLSKDLPTNYYPSRINADADGNLLISYCGSITFSGGGALYKYNPKTGEATDISPTTNHAYGAVYGDPKDANKLIATSCDFGYSQMWDANAWKNDTVAWGSQFYRSTDGGATWESITPGNETTWGGPLQADYLQDGGRPWIRGYAVHWSGAMVLDPRDSDRFLITSGNGVFACDNTWDKLPVVYFEADGIEEVVALDMISAPGKNPYSVIGDYDGFEHLDTVNSNRYSPTMTAATGGSGSASAIAYCPQNPDVMMRCAEGKGVGYYTLDGGKNWTQMECPQGGKAAITQLEDGTYRFFKSSGDNATNVSYSDDYGKTWTACSGIASAYGSKPTFMMAEKNDPSIVYAYSTYYNSSWGYSKPAPDLSDACYKFYVSHDYGKTFSEETDVAMYDQCDSAGRIAYLGDGELMLGAGYYGAYHVTDYGKKVEKLDNVSYCKTIGYGAPEKEGGVNTLYMYGKPSEDDVEGVYRSTDAGETWVAINLHHLYGGTGNGNFLVGDMNKFGMVYMSTVGCGIVYMDSSDGTDPKPVTTTTTTATTTTTTTATLTTTKAADTTTTDKDTTTTAAVVTTTDKSTTTSNAATTTQDIGEVNYGDVNVNGVVDLTDAILLNKYLAKLIQLTDIQYANANCDQTDGTDNVGDLDTTALMRFVLNVEGYQELPYIAES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5UXI1</accession>
    <name>R5UXI1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00585</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000007">
        <property type="protein sequence ID" value="CCZ82780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82780">
        <property type="protein sequence ID" value="CCZ82780"/>
        <property type="gene ID" value="BN566_00585"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="207" mass="23807" checksum="094CCBB4C5EAE7D2" modified="2013-07-24" version="1">MLRRCAFPAWHFLWFWICSLIRSWLVCVVSILIPCIPGLILICFHIQKSGILSASQIFSFPAWHFLWFWICSLIHSWLVCVVSISISCISDLILTCSRIQKKDISATFQIFSKKKKVPKNQHFFIYYPCFLDINTTHSTCIETDWIFSKKKKVPKNQHFFTHHPYFLDINTTHSTCPVPGNKSTGCTFCTTYPISCNTAQSLAAVAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5UY14</accession>
    <name>R5UY14_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative carboxymuconolactone decarboxylase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00755</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000032">
        <property type="protein sequence ID" value="CCZ82955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00755"/>
    <dbReference type="EnsemblBacteria" id="CCZ82955">
        <property type="protein sequence ID" value="CCZ82955"/>
        <property type="gene ID" value="BN566_00755"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="SSF69118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ82955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="148" mass="17003" checksum="98FD5E56E863E197" modified="2013-07-24" version="1">MERFDNFTFDEVVNHSQLDEKNRWLSILSTLIGCQEIDEYKALLPASFHFGLTPVEVKEMVYQATAYLGIGRVFPFLKATNEIFTELGIALPVQGQATTTTENRLEKGIEAQVAIFGEHMKDFYQSGDPESKQIHYWLTDNCFGNYYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYG0</accession>
    <name>R5UYG0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01134</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000092">
        <property type="protein sequence ID" value="CCZ83358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83358">
        <property type="protein sequence ID" value="CCZ83358"/>
        <property type="gene ID" value="BN566_01134"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="135" mass="15919" checksum="7AC39DF80E379015" modified="2013-07-24" version="1">MIFQSKQKVNGAPIWEVDTDTQTVRHRNPKTGETERYVFHTDHIRYYLHHIEEKRPDLLQEIVDKGEIYKHLDDLDTKVTDAVNRQTELLMQSSRDYQVAVESGNLNAVGSIGNLLRMQAQRVVYDTMIYLWRDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>R5UYQ9</accession>
    <name>R5UYQ9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH cro/C1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01222</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000105">
        <property type="protein sequence ID" value="CCZ83448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01222"/>
    <dbReference type="EnsemblBacteria" id="CCZ83448">
        <property type="protein sequence ID" value="CCZ83448"/>
        <property type="gene ID" value="BN566_01222"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06529">
        <property type="entry name" value="S24_LexA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039418">
        <property type="entry name" value="LexA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015927">
        <property type="entry name" value="Peptidase_S24_S26A/B/C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00717">
        <property type="entry name" value="Peptidase_S24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="SSF51306"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="6"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="231" mass="25975" checksum="51A45053461B26C5" modified="2013-07-24" version="1">MFQDRLQKLREQHGYSVKEAAAALHLPYTTYNNYEKNTREPNGEILCRIAAFFSVSLDYLLGATSTHSNQTVSPEEQAVYTKLQALDSHGKRLVSMVLEEEYIRCQSQKQEQQPMRTIRSSLYRVSAGTGVLLDDDAWEEIQVPDTEEAQKADFALRIAGDSMEPIYHDGDIVLVEKTDILQVGEIGIFFVNGNGYIKKFGGDRLISLNDAYADIALTNQARCFGKVIGRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZJ4</accession>
    <name>R5UZJ4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Surface antigen BspA-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01508</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000161">
        <property type="protein sequence ID" value="CCZ83773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01508"/>
    <dbReference type="EnsemblBacteria" id="CCZ83773">
        <property type="protein sequence ID" value="CCZ83773"/>
        <property type="gene ID" value="BN566_01508"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026906">
        <property type="entry name" value="LRR_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13306">
        <property type="entry name" value="LRR_5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004394664" evidence="2">
        <location>
            <begin position="41"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="3">
        <location>
            <begin position="400"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="478" mass="51231" checksum="626B9BE1184EB196" modified="2013-07-24" version="1">MNPSYRRRFCGILAAACMLTTATVPAVSPVFSQVSLSVAAAEETDFYKGLKYLKYSDYAEVIDCDQTRTTIKIPSSIGGLPVRVIAERAFYGCKSLETVTGGGNVQVIAPNAFSSCTSLTSIGAMDSLQSIGSSAFSDCVSLSNIPSSQSLKSIGDLAFFNCVSLQSVAIPATLTTLGENVFGDCISLQTFAVENGNTAFSVENDVLMNAEKTTLFCYPPAKTGTTYSVPNTVTEIAPYAFASAADLTDVTLPTGLQTIGAWAFSQTKLTSITIPNTVTTIGSYAFCNAASLKQVQLPNSLQELQAAAFWGCSSLEQVTLPNTLQEIPIYAFYGCTGLQKLTVPSSVQTIASEAFQGMFQKITVACYQNSYAETYFQKIISSDNSQGRESRYTLQTMETPTILKGDANQDGEVNVEDAVFVLQYYAKKAAGNPVSVTEAVYQAMNVDDSDDQIDVSDAVKILTYYAKKAAGQNPDWNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZP4</accession>
    <name>R5UZP4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Smr domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01558</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000165">
        <property type="protein sequence ID" value="CCZ83823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83823">
        <property type="protein sequence ID" value="CCZ83823"/>
        <property type="gene ID" value="BN566_01558"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002625">
        <property type="entry name" value="Smr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036063">
        <property type="entry name" value="Smr_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01713">
        <property type="entry name" value="Smr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00463">
        <property type="entry name" value="SMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160443">
        <property type="entry name" value="SSF160443"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50828">
        <property type="entry name" value="SMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Smr" evidence="1">
        <location>
            <begin position="27"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="97" mass="11003" checksum="EC174EB2138E62B0" modified="2013-07-24" version="1">MKRYPSKPKPMPTSTSFDIRPRHVVTIDLHGLSVAEARAKLKQVLEHCPKTVTEVEVIHGYHSGQALQKLVRKDFKHARIASRSFGLQNGITNYILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZS0</accession>
    <name>R5UZS0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Collagenase and related proteases</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01583</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000167">
        <property type="protein sequence ID" value="CCZ83848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01583"/>
    <dbReference type="EnsemblBacteria" id="CCZ83848">
        <property type="protein sequence ID" value="CCZ83848"/>
        <property type="gene ID" value="BN566_01583"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020988">
        <property type="entry name" value="Pept_U32_collagenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001539">
        <property type="entry name" value="Peptidase_U32"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12392">
        <property type="entry name" value="DUF3656"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01136">
        <property type="entry name" value="Peptidase_U32"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01276">
        <property type="entry name" value="PEPTIDASE_U32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3656" evidence="1">
        <location>
            <begin position="308"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="688" mass="74574" checksum="6604F01A9CAB0566" modified="2013-07-24" version="1">MRQSPEILAPAGSMEALTAAVHCGADAVYIGATLFSARQNAHNFDAAALQEAADFCHTNGVKLYLTVNTLVFDTEWTALDELLQTAAAIGMDACIVQDLGVADYIHQRIPEMPLHASTQMTICSPSGAIWAKEHGFSRVVVAREMTRSEIQAVCAIGLEVEQFVHGALCMCISGQCYLSALIGARSANRGCCAQACRLPFTAAKNPQAAALSLKDLCLLPHYQQLCADGIASLKIEGRMKRPEYVAAAVTALRQLKAGKQPDLQMLRAVFSRSGFTDGYYTGQRKQMFGVRQKEDVTAANAVLPKLAELAKKPAVRLPLTMAATVILEQPVSLTATLPDGTSVTVQDAPPELAKNKPCDAAFLERQLGKLGNTAYQLDSLTAICDGKATVSAATLNALRRTAIEQLQAARKAANTPQYTLAEVPLHLPKQPHSAPKKPNYWVQVQTMEQLQAVQNSDFPTDKLLLPLHLAEQLSQPIPNAILTLPTFAPDETTLRKRLQACQAIGWNAILCDTIAHLVLGKQLGLELHGGTGLNLTNRHSVNVIQQYGVSDTLLSVELTIRQALSCCDRLPAGIFAYGHLPVMKTRLCPIREEVGCRSCKHQLKDRTNRTFPVFCTEGYTVIYNAVPVWVADRFQQLRGFSTLLLSFSIESPKQIQAILADYQAPCASAPAAYTRGLLLRGLPSVVGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5UZX3</accession>
    <name>R5UZX3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2 6-diaminopimelate ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01632</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000172">
        <property type="protein sequence ID" value="CCZ83903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01632"/>
    <dbReference type="EnsemblBacteria" id="CCZ83903">
        <property type="protein sequence ID" value="CCZ83903"/>
        <property type="gene ID" value="BN566_01632"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004101">
        <property type="entry name" value="Mur_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02875">
        <property type="entry name" value="Mur_ligase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="SSF53244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mur_ligase_C" evidence="1">
        <location>
            <begin position="2"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="117" mass="12846" checksum="D1ED73ADBD9C2236" modified="2013-07-24" version="1">MICLFGCGGNRDKTKRPKMAKAAAAYADFLVITSDNPRDEDPNAIIQDILAGLEGSTIPYEVVVDRKEAILRGMRIAQPGDVLVLAGKGHEDYQVLPNNVHIHMDEREIVADCLTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>R5V006</accession>
    <name>R5V006_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SGNH_hydro domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01328</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000130">
        <property type="protein sequence ID" value="CCZ83560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01328"/>
    <dbReference type="EnsemblBacteria" id="CCZ83560">
        <property type="protein sequence ID" value="CCZ83560"/>
        <property type="gene ID" value="BN566_01328"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013830">
        <property type="entry name" value="SGNH_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13472">
        <property type="entry name" value="Lipase_GDSL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="SGNH_hydro" evidence="3">
        <location>
            <begin position="95"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="291"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="305"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="320" mass="35105" checksum="41651A3649C0559C" modified="2013-07-24" version="1">MEQKQIFGVILGVIVCIVMLFAGLYLTGQIGMKKNVVPAAAEVQTTETTDTTMTAAETTAETTTVTTTTAQSAESLSAAEKERLHTIYGEKMTVVGDSIAYGFNAYGYISDEQNLAQGSVGIRNIHDFTFTDNDGQEVDVLDALEERQPAYIYMSMGMNDVNMCTEEEYINYYQEAISSIQQLCPNSNIIVAGITPVASDSDYTDNAEIRKFNDGLEQMIADLQQPNVVYFDAYSIVADPDTQDMNPDYSGGDGIHLASTVYQELLDAVYPILDTMPIPDSIRELLQQSDFSAATEETTEETDFDSGADSGNWTDSETYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5V056</accession>
    <name>R5V056_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Acetyltransferase</fullName>
            <ecNumber evidence="4">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_00157</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="4"/>
    <dbReference type="EMBL" id="CBAR010000146">
        <property type="protein sequence ID" value="CCZ83630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00157"/>
    <dbReference type="EnsemblBacteria" id="CCZ83630">
        <property type="protein sequence ID" value="CCZ83630"/>
        <property type="gene ID" value="BN566_00157"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016407">
        <property type="term" value="F:acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039369">
        <property type="entry name" value="LacA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024688">
        <property type="entry name" value="Mac_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43017">
        <property type="entry name" value="PTHR43017"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14602">
        <property type="entry name" value="Hexapep_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12464">
        <property type="entry name" value="Mac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01266">
        <property type="entry name" value="Mac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Mac" evidence="5">
        <location>
            <begin position="4"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ83630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="206" mass="23178" checksum="EB4AE315009EDCB2" modified="2013-07-24" version="1">MENKDRMFTGDLYLPNDAAITEEQNACLELLYTFNQTRPSEQEKRNQLLKQMFAEIGENCYIEPPFHANFGGKHVHFGKNVYANFNLTMVDDGHIYVGDYTMFAPGVIVATAGHPILPELRETVYQYNMPVHIGKNCWIGAGAIILPGVTIGDQVVVGAGSVVTKDLPSNVVAVGNPCRVVREVSEHDRVYYFKNRKIDWDTLPRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0B3</accession>
    <name>R5V0B3_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01441</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000154">
        <property type="protein sequence ID" value="CCZ83705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83705">
        <property type="protein sequence ID" value="CCZ83705"/>
        <property type="gene ID" value="BN566_01441"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032774">
        <property type="entry name" value="WG_beta_rep"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14903">
        <property type="entry name" value="WG_beta_rep"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="366" mass="40924" checksum="1240AA198C4EB9E3" modified="2013-07-24" version="1">MRPHTQTARKDTDSAPKKGMWLFVFFLLLIAGLGIAVSRFYAAKTEDPSASHHVSSVPEQDSCYIFQEESTGLYGLSDADGRIIAAAEWKNLSFIGDHGVAAVKQIGTESVMGVLDREGNVIVPFVYQDLSYVNNQILVGKLLHEDLYFLYDRSFQDLTNCTWNAYLLKDDGLTLQRGTDSFFYQQIKDGIVLRSFDLQRTIQRKVVTMHCEHRVTAQRYPVEVWQMLGDDLSEFLKAYQRNDETAAAAFLSSDAISQLHPLIPKNKAEKWLVDWNSSIGISSQMTQDNTVFCTITVKRDTESAVLRVAFSTESTAGTLQITGLEILQEKAEKTEESSALETTADFVINDGGRDISANPFGSVQEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0G8</accession>
    <name>R5V0G8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cell envelope-related function transcriptional attenuator common domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">BN566_01500</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LytR/CpsA/Psr (LCP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000161">
        <property type="protein sequence ID" value="CCZ83765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01500"/>
    <dbReference type="EnsemblBacteria" id="CCZ83765">
        <property type="protein sequence ID" value="CCZ83765"/>
        <property type="gene ID" value="BN566_01500"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004474">
        <property type="entry name" value="LytR_CpsA_psr"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03816">
        <property type="entry name" value="LytR_cpsA_psr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00350">
        <property type="entry name" value="lytR_cpsA_psr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="LytR_cpsA_psr" evidence="4">
        <location>
            <begin position="129"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="385"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="409" mass="46237" checksum="45962D778678744C" modified="2013-07-24" version="1">MAKKRQTKKSQKNTKKKDLFVTIGSIALTVVLVVTMILNAPIIDYRTITNGTMSTRQISIMKYFKVWQPLVQKEGTLQKPVNTDASALNLKEDTDDADPKDDGLDLDQIVEGQYTVLFMGMDESGKLADVNWIFQFDIFAGTMNVLQIPRDCYMPAYANTSTNKFNSIYGTGQDETVTPIQRAVNAIQESFGLVIDCYVKLSCTDIANIVDSIGGIPIDLPEEIMYEADKVLKPGKQTLNGQQAEWFVRFRREYDEGDIGRVKAQRIFLAAAMQKLLDMSQTELMSAMQKIYKNEWIATDLSLEQISMLADFAAKRLTMDGVNVYMVPGEGIMYEGQAVYSIHKTEAIDLINEYFRPYQNEMYYDESTIVELVDESNYIDTYSDTEENLGDIHNGDTEDGKQNIYSNGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5V0X4</accession>
    <name>R5V0X4_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">dGTP triphosphohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00182</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000198">
        <property type="protein sequence ID" value="CCZ84253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00182"/>
    <dbReference type="EnsemblBacteria" id="CCZ84253">
        <property type="protein sequence ID" value="CCZ84253"/>
        <property type="gene ID" value="BN566_00182"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026875">
        <property type="entry name" value="PHydrolase_assoc_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13286">
        <property type="entry name" value="HD_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="1">
        <location>
            <begin position="73"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="410" mass="48151" checksum="A6AAF73B68CF69A8" modified="2013-07-24" version="1">MVPGLFQAYASSETNPNWKKQIERQQELYKREYDIRSEYERDYTRILHSLAYRRLKHKTQVFFNIDNDHICTRMEHVQHVESVSCTIAKYLGLNVDLTRAIAMGHDLGHAPFGHEGEVELTAIRNEYGFDDFWHERNSLRMVDNIELLQDNQKNYRNLNLTYAVRDGIISHCGEVDQNGIFPRKEAINLSEFSSPGKFQPYTWEGCVVKIADKIAYLGRDIEDAIRMHFIDEKDFRKLIEISKKYGEKVINTTVIIHNFITSICENSSPNLGIRLSNRHNEMLKEIKDYNTIAIYKNHKFDVYRKYAALIIRSVFKTLMDAYSKENTPKQLKKMIKTYPILIKDFLQHIDQYSTLDPNNPQIAKKVLRCKNNKIYGNLETEEIYAQAIVDFISGMTDRYAVTVFNELLRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>R5V107</accession>
    <name>R5V107_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cellulase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00022</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000208">
        <property type="protein sequence ID" value="CCZ84283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00022"/>
    <dbReference type="EnsemblBacteria" id="CCZ84283">
        <property type="protein sequence ID" value="CCZ84283"/>
        <property type="gene ID" value="BN566_00022"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002105">
        <property type="entry name" value="Dockerin_1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016134">
        <property type="entry name" value="Dockerin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024745">
        <property type="entry name" value="Glyco_hydro_44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00404">
        <property type="entry name" value="Dockerin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12891">
        <property type="entry name" value="Glyco_hydro_44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="SSF63446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51766">
        <property type="entry name" value="DOCKERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004394708" evidence="2">
        <location>
            <begin position="33"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="domain" description="Dockerin" evidence="3">
        <location>
            <begin position="765"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="705"/>
            <end position="744"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="837" mass="91386" checksum="4BFD41CA00A63AE4" modified="2013-07-24" version="1">MQHRKRTALIAGLFTITCLAQISMNTMSGISADAAGSYDMAVTVNLAGEKKAISPHIYGVNDSGDGSNLKNVTVDTVRQGGNRLTGYNWETNYSNAGEDWHNSSDTNIGDDTDGCGYAGRRLSATCQKNNIPYKMTTLQMAGYVAADKAGTVLESEAAPSSRWKEVKFKKDTALTLEPDVTDDYVYMDEYVNYLVKTLGDSTTSTGIQAYSLDNEPVLWNDTHPLLHSKEVSSSELISKSIELANVVKEIDPNAEVFGPAFWGMLPCINGSNSASDKNNNVYTDPDYDAVKGNYSWFMDYYLEQMANAEKESGKRLLDVVDVHFYSQDCSTEASRVQAARSLYDASYVENSWLQPTFGQYFPFLPKLQESIDKYYPGTKIAISEYNFADLSNEKESGKLSSAAIAEADALGCFADNNVYFATYWGTLSECPYAASAINLYTNYDGEGASFGDTLVESSTSDISLAYSYASIDGSDDSTVKTVLSNKSADQTEDAVITLDGTTKDYQSAVVYAITPKDDQIRIIDVQNDISGNQVKVELPPMSVAQVVVSDQKTDKEVYVKPEEPDTKTITYKYEDLELSGNGFPKIPLTDLEHLKKVIINTTVTCSTNADWYGGGGALAFNDLLLEDGTKAWASKAYSFGAGTNDNVILMDGKYTVNKEEVSGTPTQDYAELQGCWWKSSTNSESGEDVSVTFNSVSLVYEYEKDPDTTTTTTTTTTTTTETTTTLTETTPAETTAPDETTTSIESTTSEIIETTTAETTTIDDSNISYGDVNTDGVVDLRDAVYLNKHLATLVTLTDIQLKNADVYADGNVTEDDTTYLMQFVLNMITDLPVPPKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>R5V154</accession>
    <name>R5V154_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ribosome-recycling factor</fullName>
            <shortName evidence="3">RRF</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Ribosome-releasing factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">frr</name>
        <name evidence="6" type="ORF">BN566_01719</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the RRF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000178">
        <property type="protein sequence ID" value="CCZ83990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01719"/>
    <dbReference type="EnsemblBacteria" id="CCZ83990">
        <property type="protein sequence ID" value="CCZ83990"/>
        <property type="gene ID" value="BN566_01719"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006415">
        <property type="term" value="P:translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00520">
        <property type="entry name" value="RRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00040">
        <property type="entry name" value="RRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002661">
        <property type="entry name" value="Ribosome_recyc_fac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023584">
        <property type="entry name" value="Ribosome_recyc_fac_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036191">
        <property type="entry name" value="RRF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20982">
        <property type="entry name" value="PTHR20982"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01765">
        <property type="entry name" value="RRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55194">
        <property type="entry name" value="SSF55194"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00496">
        <property type="entry name" value="frr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RRF" evidence="5">
        <location>
            <begin position="21"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ83990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="184" mass="20873" checksum="4B68DA13B7965142" modified="2013-07-24" version="1">MKTILKDAESKMQKALDRLDYEYSTIRAGRANPAVLEKVSVDYYGAMTPINQMAAVSVAEARILVIQPWDVSTLKAIEKAILNSDIGITPTNDGKVLRLVFPQLTEERRKDLCKSIKKYGEECKVAVRNVRREAMDKMKAKKKDGELTEDDMKSGEKDVQKLTDKYCDKVDQHVSDKEKEIMSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>R5V161</accession>
    <name>R5V161_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin)</fullName>
            <ecNumber evidence="4">1.17.7.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ispG</name>
        <name evidence="5" type="ORF">BN566_01724</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H(+) + H2O + oxidized [flavodoxin] = 2-C-methyl-D-erythritol 2,4-cyclic diphosphate + reduced [flavodoxin]</text>
            <dbReference type="Rhea" id="RHEA:43604"/>
            <dbReference type="Rhea" id="RHEA-COMP:10622"/>
            <dbReference type="Rhea" id="RHEA-COMP:10623"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:58483"/>
            <dbReference type="ChEBI" id="CHEBI:128753"/>
            <dbReference type="EC" id="1.17.7.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="4">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 5/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the IspG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.17.7.3" evidence="4"/>
    <dbReference type="EMBL" id="CBAR010000178">
        <property type="protein sequence ID" value="CCZ83995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01724"/>
    <dbReference type="EnsemblBacteria" id="CCZ83995">
        <property type="protein sequence ID" value="CCZ83995"/>
        <property type="gene ID" value="BN566_01724"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00056">
        <property type="reaction ID" value="UER00096"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046429">
        <property type="term" value="F:4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019288">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.413.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00159">
        <property type="entry name" value="IspG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016425">
        <property type="entry name" value="IspG_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004588">
        <property type="entry name" value="IspG_bac-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045854">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30454">
        <property type="entry name" value="PTHR30454"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04551">
        <property type="entry name" value="GcpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004640">
        <property type="entry name" value="IspG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="SSF51717"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56014">
        <property type="entry name" value="SSF56014"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00612">
        <property type="entry name" value="ispG_gcpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 4" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="344" mass="36549" checksum="CC25530540C83CE1" modified="2013-07-24" version="1">MQKKAVRVGDCVLDGKHIYIQSMLNTRSDDIAGSVAQAKALEQAGCEILRAAIPDKEALKLIPAIKEAVKIPLVADIHFDYRLALEAVAAGIDKIRINPGNIGGMDRVRQVVAACQSKQIPIRIGVNSGSLEKDLLAKYGAPTAEALVESAMRHVKMLEDCDFTDIVISMKSSTVSTMIDAYRLAAQQCAYPLHLGVTEAGTAHMGLIKSAIGIGALLHDGIGATIRVSLTDDPIQEITAAKDILKCMGLRGGPELVACPTCGRTRIDLVQTAKEVEAALANVDKDIKVAVMGCVVNGPGEAREADIGIAGGDGCAVLFKKGEVLRKIPESEIVTELMKEIEKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1U7</accession>
    <name>R5V1U7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00204</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000205">
        <property type="protein sequence ID" value="CCZ84275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84275">
        <property type="protein sequence ID" value="CCZ84275"/>
        <property type="gene ID" value="BN566_00204"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ84275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="134" mass="16040" checksum="6B288ACAF9E53D0C" modified="2013-07-24" version="1">MSIFSRNVSSGCRRLKQPERQLSLFPMRWSRLSRFVTVLFGLTMGRFGKKAFPNMSMNIIFPPWKRSGLHGKQSIHRKPLNRNPRMQMIRQLCRHFVNQRRCGWEMVQCSLPMQRCWMQMGIPVMRFRQGQQLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1V1</accession>
    <name>R5V1V1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00402</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000240">
        <property type="protein sequence ID" value="CCZ84503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00402"/>
    <dbReference type="EnsemblBacteria" id="CCZ84503">
        <property type="protein sequence ID" value="CCZ84503"/>
        <property type="gene ID" value="BN566_00402"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030949">
        <property type="entry name" value="ECF_S_folate_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024529">
        <property type="entry name" value="ECF_trnsprt_substrate-spec"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12822">
        <property type="entry name" value="ECF_trnsprt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04518">
        <property type="entry name" value="ECF_S_folT_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="178"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="209" mass="22934" checksum="2DE2532FE7651630" modified="2013-07-24" version="1">MKGIFSCFAESAKEFTKLRSLVITALFIAISMIIEAFSIDLQFAKLNFAFLAIAVIGMLFGPCMGLVAGFACDIVGYLVHPTGGFLPAYILVAGLQGLIYGLCLYHKMNGHSIQFRNTTTGKEWDITLFLRAVVARLLDVIVINLLINTKLNLHYGFIPEQAYGAAIIARTAKNVIELALDLPMLFILLPIALTAYQRVFRLQKKAAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1V5</accession>
    <name>R5V1V5_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Predicted membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">BN566_00054</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UPF0702 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000241">
        <property type="protein sequence ID" value="CCZ84508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00054"/>
    <dbReference type="EnsemblBacteria" id="CCZ84508">
        <property type="protein sequence ID" value="CCZ84508"/>
        <property type="gene ID" value="BN566_00054"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.240.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007353">
        <property type="entry name" value="DUF421"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023090">
        <property type="entry name" value="UPF0702_alpha/beta_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04239">
        <property type="entry name" value="DUF421"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ84508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="231" mass="25973" checksum="C2EBBFD3CA0C568F" modified="2013-07-24" version="1">MGTIFIRALILYFVVIFSVRLMGKRQLGELQPSELVITILISNIATLPLEDTAIPLLPGILPILSMVCFEVIISWFSLKSRRLRRVICGSPKIIIRNGQIEQDTMRELRLSIDDLMTALRGNQVFSIEEVQFAIVETTGSISVYLKKEHQPFTLQDADLHPKTIDPPVTLIADGTLSRNALHSLQKEHSWVAAELQRRKLQEKDVFLLTADRNGICTCIPKQFHEKGGAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1V7</accession>
    <name>R5V1V7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC transporter permease/ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN566_00024</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000208">
        <property type="protein sequence ID" value="CCZ84285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00024"/>
    <dbReference type="EnsemblBacteria" id="CCZ84285">
        <property type="protein sequence ID" value="CCZ84285"/>
        <property type="gene ID" value="BN566_00024"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="PTHR24221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="134"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="158"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="246"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="276"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="17"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="335"/>
            <end position="571"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ84285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="580" mass="63428" checksum="D68148BF5B0AA65A" modified="2013-07-24" version="1">MLKRLAGCVRQYKGASLLSPLFVVLEVIMEVIIPLLMAKLIDDGIDGANQAAILKTGGILAVCCILSLLFGILAGHFAAKASAGFAKNIRHDLFHVVQGFSFFNIDRFSASSLVTRLTTDVTNLQNAYQMIVRVALRSPAMLIFSLAMAIRISPKLSIIFLIAIPILGAFVFFLMSSVHPIFERVFRTYDKLNNVVQENVHGVRVVKAFVREDHEISKFAKISQKIYADFCKAEGRLSFSMPGMQVAVYSCMLLLSWFGARIIVGSGGSDLTTGELMSLMTYALQILGALMMLSMIFVMVVISRASAERIVEVLDEQSDLTNPEHPVLKVADGSVDFSHVSFSYSKRMDKCCLKDIDLHIPSGMTVGILGGTGSSKSSLVQLIPRLYDTTAGTVKVGGVDVKDYDLDTLRNAVAMVLQKNVLFSGTIKENLRWGNEQATDAELQEACRLANADEFIATFPKGYDTYLEQGGANVSGGQKQRLCIARALLKKPKILILDDSTSAVDTKTDAGIRTALKTYLPETTKFIIAQRISSLQDSDLILVLDGGVINGMGTHEELLQTNAIYQEIYEEQTKKGGVTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>R5V1Y9</accession>
    <name>R5V1Y9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phosphoserine aminotransferase</fullName>
            <ecNumber evidence="6">2.6.1.52</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Phosphohydroxythreonine aminotransferase</fullName>
            <shortName evidence="6">PSAT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">serC</name>
        <name evidence="8" type="ORF">BN566_00404</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>2-oxoglutarate + 4-(phosphooxy)-L-threonine = (R)-3-hydroxy-2-oxo-4-phosphooxybutanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:16573"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58452"/>
            <dbReference type="ChEBI" id="CHEBI:58538"/>
            <dbReference type="EC" id="2.6.1.52"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 6">
            <text>2-oxoglutarate + O-phospho-L-serine = 3-phosphooxypyruvate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:14329"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:18110"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57524"/>
            <dbReference type="EC" id="2.6.1.52"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
        <text evidence="6">Binds 1 pyridoxal phosphate per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 6">Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 6">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. SerC subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.52" evidence="6"/>
    <dbReference type="EMBL" id="CBAR010000242">
        <property type="protein sequence ID" value="CCZ84543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00404"/>
    <dbReference type="EnsemblBacteria" id="CCZ84543">
        <property type="protein sequence ID" value="CCZ84543"/>
        <property type="gene ID" value="BN566_00404"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00135">
        <property type="reaction ID" value="UER00197"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004648">
        <property type="term" value="F:O-phospho-L-serine:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00611">
        <property type="entry name" value="PSAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00160">
        <property type="entry name" value="SerC_aminotrans_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022278">
        <property type="entry name" value="Pser_aminoTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43247">
        <property type="entry name" value="PTHR43247"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000525">
        <property type="entry name" value="SerC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01364">
        <property type="entry name" value="serC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 8" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0718">Serine biosynthesis</keyword>
    <keyword evidence="6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_5" evidence="7">
        <location>
            <begin position="4"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="6">
        <location>
            <begin position="76"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="6">
        <location>
            <begin position="238"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="6">
        <location>
            <position position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ84543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="361" mass="40013" checksum="D3F0A9E175998FEC" modified="2013-07-24" version="1">MKRVYNFSAGPAVLPEEVLREAADEMLDYNGTGMSVMEMSHRSKAFEEIIQTAEQDLRDLMHIPDNYTVLFLQGGASQQFSAIPMNLMKNGVADYIVTGQWAKKAYQEAQKYGKAVKIASSEDETFSYIPDCSDLPIDPDADYVYICENNTIYGTKFKTLPNTKGKPLVADVSSCFLSEPVDVSKYGVIWGGVQKNIGPAGVVIMIIRNDLIPEETPIANTPTMLKWKTQADAKSLYNTPPAYGIYICGKVFKWLKKMGGLEAIQKRNEEKAAILYNYLDQSKLFKGTVRKEDRSLMNVPFVTGDKDLDAKFVAEAKAAGLENLKGHRTVGGMRASIYNAMPVAGVEALVAFMKKFEAENV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5V2D8</accession>
    <name>R5V2D8_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ribose-5-phosphate isomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00369</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LacAB/RpiB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000238">
        <property type="protein sequence ID" value="CCZ84470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00369"/>
    <dbReference type="EnsemblBacteria" id="CCZ84470">
        <property type="protein sequence ID" value="CCZ84470"/>
        <property type="gene ID" value="BN566_00369"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016861">
        <property type="term" value="F:intramolecular oxidoreductase activity, interconverting aldoses and ketoses"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1400.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004785">
        <property type="entry name" value="RpiB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003500">
        <property type="entry name" value="RpiB_LacA_LacB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036569">
        <property type="entry name" value="RpiB_LacA_LacB_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02502">
        <property type="entry name" value="LacAB_rpiB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005384">
        <property type="entry name" value="RpiB_LacA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89623">
        <property type="entry name" value="SSF89623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01120">
        <property type="entry name" value="rpiB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00689">
        <property type="entry name" value="rpiB_lacA_lacB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="D-ribulose 5-phosphate binding" evidence="3">
        <location>
            <begin position="8"/>
            <end position="9"/>
        </location>
    </feature>
    <feature type="region of interest" description="D-ribulose 5-phosphate binding" evidence="3">
        <location>
            <begin position="65"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="97"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>D-ribulose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>D-ribulose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>D-ribulose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>D-ribulose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR005384-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR005384-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="146" mass="15724" checksum="EBBD65AE26DCA6A0" modified="2013-07-24" version="1">MKIWIGCDHAGYPMKLEVLAMLQEQGYTVEDMGCDGSRADYPEIAAAVGKEVAANPADKGILICGTGIGMSIAANKIRGVRAALCADHFSAKYTRLHNDANIICMGARTLGSGVALELVDLFLHTPFEGGRHEKRVAMIKDLENNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5V2T7</accession>
    <name>R5V2T7_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein conserved in bacteria</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00446</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0297 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000247">
        <property type="protein sequence ID" value="CCZ84585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00446"/>
    <dbReference type="EnsemblBacteria" id="CCZ84585">
        <property type="protein sequence ID" value="CCZ84585"/>
        <property type="gene ID" value="BN566_00446"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009309">
        <property type="entry name" value="IreB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40067">
        <property type="entry name" value="PTHR40067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06135">
        <property type="entry name" value="IreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037258">
        <property type="entry name" value="DUF965_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="81" mass="9389" checksum="4759742B90D954C1" modified="2013-07-24" version="1">MYDKTMTFSVNDEKEIEMKKTLTAVYDALVQKGYNPVNQIVGYILSEDPTYITTYGNARSLIRHIDRDELLQALVKSYLNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>R5V3W9</accession>
    <name>R5V3W9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Chaperone protein ClpB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">clpB</name>
        <name evidence="12" type="ORF">BN566_00609</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homohexamer. The oligomerization is ATP-dependent.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homohexamer; The oligomerization is ATP-dependent.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the ClpA/ClpB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000008">
        <property type="protein sequence ID" value="CCZ82804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00609"/>
    <dbReference type="EnsemblBacteria" id="CCZ82804">
        <property type="protein sequence ID" value="CCZ82804"/>
        <property type="gene ID" value="BN566_00609"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1780.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017730">
        <property type="entry name" value="Chaperonin_ClpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036628">
        <property type="entry name" value="Clp_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004176">
        <property type="entry name" value="Clp_R_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001270">
        <property type="entry name" value="ClpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018368">
        <property type="entry name" value="ClpA/B_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028299">
        <property type="entry name" value="ClpA/B_CS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041546">
        <property type="entry name" value="ClpA/ClpB_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17871">
        <property type="entry name" value="AAA_lid_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02861">
        <property type="entry name" value="Clp_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00300">
        <property type="entry name" value="CLPPROTEASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81923">
        <property type="entry name" value="SSF81923"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03346">
        <property type="entry name" value="chaperone_ClpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51903">
        <property type="entry name" value="CLP_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00870">
        <property type="entry name" value="CLPAB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00871">
        <property type="entry name" value="CLPAB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="Clp R" evidence="11">
        <location>
            <begin position="3"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="416"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00026057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CCZ82804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="866" mass="97816" checksum="06CC8850556EB324" modified="2013-07-24" version="1">MNAQKLTEKSLEAIQSANDMAISHQNQMIQQVHLLCALLEQENGLIPQLFEKVGVSKETAVRDLEQQINQLPSVTGSGRKADSVYIAQDVDMVLTDAEKQADKMRDAYVSVEHLMLSLFDHANADVKKLLQKWNLKKADFMKALMAVRGNQQVTGQNPEETYDVLKKYGQDLVQLARNNKLDPVIGRDSEIRNVIRILSRKTKNNPVLIGEPGVGKTAIAEGLALRIVRGDVPDNLKDRILFSLDLGALVAGAKYRGEFEERLKAVLNEIKKSEGKIILFIDELHTIVGAGKTDGAMDAGNLLKPMLARGELHCIGATTLNEYRQYIEKDAALERRFQPVMVNEPTVEDTISILRGLKERYEVFHGVKIQDQALIAAATLSDRYITDRFLPDKAIDLIDEACALIRTEMNSMPTEMDEIQRKIMQHEIEEAALKKEDDKLSQAHLQEVQQELAQMREQFHAMKAKWENEKANISKVQKLREELESVNAQIAKAEREYDLNKAAELKYGKLPSLKKELEAEEAKAEAAKEQSDNLLRDKVTEEEIARIICRWTGIPVARLMEGEREKLLHMEDILHRRVIGQDEAVEKVSDAILRSRAGIQDPNRPLGSFLFLGPTGVGKTELAKALAEALFDDENNIVRIDMSEYMEKFSVSRLIGAPPGYVGYEEGGQLTEAVRRKPYSVVLLDEIEKAHPDVFNILLQVLDDGRITDSQGRTVDFKNTIIILTSNLGSPYILDGINADGEISEEARQKVDQLLKQSFRPEFLNRLDEIVFYKPLMKTEIYKIVDLMLNSLRKRLEDKQLSLTVTDAAKDYIIANGYDVNYGARPLKRWIQQKVETLLARKIIAEDLAPNTMLTVDYDGNKLIVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>R5V3X2</accession>
    <name>R5V3X2_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_00614</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000009">
        <property type="protein sequence ID" value="CCZ82809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00614"/>
    <dbReference type="EnsemblBacteria" id="CCZ82809">
        <property type="protein sequence ID" value="CCZ82809"/>
        <property type="gene ID" value="BN566_00614"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032524">
        <property type="entry name" value="ABC_tran_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037118">
        <property type="entry name" value="Val-tRNA_synth_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16326">
        <property type="entry name" value="ABC_tran_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="48"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="294"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="80"/>
            <end position="87"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ82809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="362" mass="41227" checksum="44986CA36BE6EA7F" modified="2013-07-24" version="1">MRRGVRARGTKSKDRMERFYALENREKPVETESLQLQSVSSRLGKKTIELEHLTKSIQGNVLLQDFSYQLPRAARIGIIGRNGAGKSTLLKLLAGILQPDSGTITWGDTVQIGYFAQECETMNPTQKVIDYIRETAERIQTPEGTVTAATMLERFLFTPELQWNRIEKLSGGERKRLYLLKILMTAPNILLLDEPTNDLDIVTLTILEDYLKSFSGAVIAISHDRYFLDKMASEIWELPGDGSVRRYNGNYSDYATNAMPTAPAESEKKSTEKKEKTFVGKKKLKFSYKEQREYETIESVIADLEEQIQETETKIAAAVSDYVLLQELSDQKDALEEQLAEKMDRWVYLSDLAERIEKGETV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5V458</accession>
    <name>R5V458_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00706</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000022">
        <property type="protein sequence ID" value="CCZ82904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ82904">
        <property type="protein sequence ID" value="CCZ82904"/>
        <property type="gene ID" value="BN566_00706"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022476">
        <property type="entry name" value="Spore_YabP/YqfC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07873">
        <property type="entry name" value="YabP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ82904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="88" mass="10401" checksum="4EEE3E4F32F33660" modified="2013-07-24" version="1">MLERWTEWGRKWFHLQPMLHVAGNQELRIENCRHILEYNDIRIVIQTQDLQISVWGKSLQVDTYATDGVLIHGTLQSIELEQRGGRCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5V468</accession>
    <name>R5V468_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosidases</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00714</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000026">
        <property type="protein sequence ID" value="CCZ82914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00714"/>
    <dbReference type="EnsemblBacteria" id="CCZ82914">
        <property type="protein sequence ID" value="CCZ82914"/>
        <property type="gene ID" value="BN566_00714"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.400.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023387">
        <property type="entry name" value="DUF1653-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="Glyco_hydro_13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045857">
        <property type="entry name" value="O16G_dom_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07866">
        <property type="entry name" value="DUF1653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aamy" evidence="1">
        <location>
            <begin position="11"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="523" mass="60266" checksum="54E02CEAF0209EAA" modified="2013-07-24" version="1">MNWYDNAIMYHIYPLGFCGAPKHNEGGEPVNRLEKVLDWIPHLKEMQVDAVYFGPIFESVEHGYDTTDYRQIDRRLGTNEMFKHICEVLHENGIRVILDGVFNHVGRNFWAFRDVQEKGQASPYCDWFAGLNFGGSSPYGDPFWYEGWNGYYNLVKLNLKNWHVCDHLIEAVGYWMDEFQIDGIRFDAADCVDFDFFKRIRSFCKGKKPDFWLMGEIIHGDYKRWANPEMLDSVTNYECYKGIYSSHNDKNYFEINYSINRQFGNGGIYQGINLYNFVDNHDVNRLASTLVDQRYLPLCYTLMYAMPGIPSVYYGSEYGVQGRHENNSDDGLRPCLDLADLQRSGNLDLYRHLVKLGRIYKAYPALRTGSYETVIVRNRQLLFRKDWDGTTVYVALNLEDCCSDMQFGTHLPALVDVISGQPIDVNNGNVNVHMQPFSSMILVADDIVNHADAPETVPVAAEPEKPVEAGDRYQQEDGSVWKVVSLASHVETQEELVIYQDEASGKVWAMPKSIFIDRKKTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5V497</accession>
    <name>R5V497_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glutamine synthetase type I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_00739</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the glutamine synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000029">
        <property type="protein sequence ID" value="CCZ82939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00739"/>
    <dbReference type="EnsemblBacteria" id="CCZ82939">
        <property type="protein sequence ID" value="CCZ82939"/>
        <property type="gene ID" value="BN566_00739"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamate-ammonia ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006542">
        <property type="term" value="P:glutamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008147">
        <property type="entry name" value="Gln_synt_b-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036651">
        <property type="entry name" value="Gln_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03951">
        <property type="entry name" value="Gln-synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01230">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54368">
        <property type="entry name" value="SSF54368"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="SSF55931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gln-synt_C" evidence="3">
        <location>
            <begin position="101"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ82939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="427" mass="48463" checksum="62B265BA69B9EDB2" modified="2013-07-24" version="1">MFFSESDVTEFIEDNDVKFIRLTFCDTLGAMKNIAILPRELPRAIAYGIPFNATGLLEEQHQNLLLKPECTTLSVLPWRPQSGRVVRFFCNLMTMDGKPYAGDLRQNLRNTMREIEENDYQCQIATRCEFYLFKTDVEGNPTREPYDQGGYLDIAPLDKCENTRREICLSLEEMGLNPTSSCHKHGPGQNEIDFACSNPLTAADNMMHYKTVVKTIAAQNGLYASFMPKPLPNQNGSALKINIIVRKHGQNIFGSCPEDMTPEGKSFIAGILNRICGFTLFANPIVNSYERLQLHTAPGRVNWSSENRVPLIRLMYVPGGDAIIEVRSADAYCNPYITFQMLLRAGMEGIHHQEQLKEEWDAANVTTVFQKLPTSLEEALQMARESEFVKAYLPNAILHDFFQKTEQQIQAVSEAEDAQAYSYHHFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5V4Z1</accession>
    <name>R5V4Z1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00967</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000069">
        <property type="protein sequence ID" value="CCZ83184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83184">
        <property type="protein sequence ID" value="CCZ83184"/>
        <property type="gene ID" value="BN566_00967"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="72" mass="8028" checksum="A7E8C1B399D0C4B8" modified="2013-07-24" version="1">MHFFSHKKPILPLPDGCTPEQIQIESSICTGETTIGFYDAAEKKLRYAELVKTPADIAAFYARYGLTPPDHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5V547</accession>
    <name>R5V547_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01030</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000081">
        <property type="protein sequence ID" value="CCZ83249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83249">
        <property type="protein sequence ID" value="CCZ83249"/>
        <property type="gene ID" value="BN566_01030"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="53" mass="5590" checksum="F0BC41C02B5D5F1E" modified="2013-07-24" version="1">MGSIVIGILLLVIVVGIVWKLRSDQKKGKHSCGGTCGGCPNAAHCHSQTMKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="12" xmlns="http://uniprot.org/uniprot">
    <accession>R5V5Q0</accession>
    <name>R5V5Q0_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01228</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000109">
        <property type="protein sequence ID" value="CCZ83454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83454">
        <property type="protein sequence ID" value="CCZ83454"/>
        <property type="gene ID" value="BN566_01228"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="149" mass="16602" checksum="C8DEF093AB684EC7" modified="2013-07-24" version="1">MKLEQVRCFFQMLTQLDDIQPYEALVSTAVTTVTDALKPNVDPTLQPLQLLAAAIANESYQILLATKEQTACTYAGTTPRQADRSQQITAARKLREQYQQMCSPMLLDRQFYFQRTHLNREEQSEEDAANSKPSTEPATGTADAGLSGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>R5V608</accession>
    <name>R5V608_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01293</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000121">
        <property type="protein sequence ID" value="CCZ83524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83524">
        <property type="protein sequence ID" value="CCZ83524"/>
        <property type="gene ID" value="BN566_01293"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="5"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="73" mass="8815" checksum="BFD4791556FBC10F" modified="2013-07-24" version="1">MQLRIRDLREDHDLTQKQIAAYLMCDQSLYSKYERMERELPLHLAIQLARFYHVSLDYLVGMTNEKQPYPAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-05-25" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>R5V661</accession>
    <name>R5V661_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Beta-galactosidase</fullName>
            <ecNumber evidence="3 9">3.2.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Lactase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">BN566_01367</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.</text>
            <dbReference type="EC" id="3.2.1.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glycosyl hydrolase 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.23" evidence="3 9"/>
    <dbReference type="EMBL" id="CBAR010000144">
        <property type="protein sequence ID" value="CCZ83604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01367"/>
    <dbReference type="EnsemblBacteria" id="CCZ83604">
        <property type="protein sequence ID" value="CCZ83604"/>
        <property type="gene ID" value="BN566_01367"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009341">
        <property type="term" value="C:beta-galactosidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004565">
        <property type="term" value="F:beta-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004199">
        <property type="entry name" value="B-gal_small/dom_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036156">
        <property type="entry name" value="Beta-gal/glucu_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006101">
        <property type="entry name" value="Glyco_hydro_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023232">
        <property type="entry name" value="Glyco_hydro_2_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006103">
        <property type="entry name" value="Glyco_hydro_2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023230">
        <property type="entry name" value="Glyco_hydro_2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006102">
        <property type="entry name" value="Glyco_hydro_2_Ig-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006104">
        <property type="entry name" value="Glyco_hydro_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032312">
        <property type="entry name" value="LacZ_4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02929">
        <property type="entry name" value="Bgal_small_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16353">
        <property type="entry name" value="DUF4981"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00703">
        <property type="entry name" value="Glyco_hydro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02836">
        <property type="entry name" value="Glyco_hydro_2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02837">
        <property type="entry name" value="Glyco_hydro_2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00132">
        <property type="entry name" value="GLHYDRLASE2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01038">
        <property type="entry name" value="Bgal_small_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49303">
        <property type="entry name" value="SSF49303"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="SSF74650"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00719">
        <property type="entry name" value="GLYCOSYL_HYDROL_F2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00608">
        <property type="entry name" value="GLYCOSYL_HYDROL_F2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="7 9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bgal_small_N" evidence="10">
        <location>
            <begin position="734"/>
            <end position="1006"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ83604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="1008" mass="113890" checksum="86938E562A907C65" modified="2013-07-24" version="1">MTFSTDILKNPEIFQINTCKPHSSHIHYRSAAECKEQQSSFFLSLNGDWKFFYSENLNTLPEHFEQVGYDDSNWDTIPVPGHLQTNGYGQMQYVNTMYPWDGVENCKPGEIPECFNATGSYIRMVTIPEAFRKDAEIRLVFHGAETAMALWVNGTFVGYHEDSFTPAEFAITDYLNDGENKIAVQVFQRSSGSWLEDQDFWRFSGLFRDVELYAVPKLHLQDLFVHTDLNDTFSQADVRVDCAFSGTASGTLHAVLTDPNGKQLAEQSMPVTASEQVVAFSVEQPLLWSAEQPNLYHLVLTLQDADGTVLEVVPQQIGIRQFRMENGIMKLNGKRIVFNGVNRHEFSATRGRAITKEEIRWDIFTIKQNNINAVRTSHYPNQNDFYELCDQYGLYVIDETNMETHGTWQKMGAIAPDEHTLPNDNPHWTNAVLARAAAMQERDKNHPCVLIWSCGNESFGGSNIYAMSEQFRKRDSSRLVHYEGVVNDRRFNQTSDMESQMYPSVAKIEQFLKEHPEKPFICCEYSHSMGNSNGALFKYTDLAKREPRYQGGFIWDFIDQAVFKKNEYGESFLATGGDFDDRPTDYNFCTNGIVFADRTITTKMAEVKYCYQPFTIACTPQGFTIRNEFLFTDTTPYSMTATLCKNGVPVAEQELSLPVIAPQTTQSFANPFAGTATEPGAYTLTISIYRTAATDWADAQTEVAFGQAAWNVVVPTTEELLPAPELIVGDVNYGIHGSDFHILFGRDKAGIVSYQKNGVERIASQVGIPRPNFWRAPIENDLGCRMPHDTAMWKIASLYAVPSIVSCKQTAHTVDIVTRYTFPQNPDNGCLVTYQFYGDGSITVTMQMLGCTEYGNMPDFGMLLQMPLSFDQIAWYGRGKDETYCDRKNGSRIGCYTSTVAEQLVPYAYVQECGNHTDTQWFTITNADGHGFRFSAEQMDFSALPYTPHQLEQAYHLQELPRSYRNVVRISAGEMGVGGDDSWGATVHPEFTRQSKTGLSFTFRMTAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>R5V6C6</accession>
    <name>R5V6C6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phi13 family Major tail protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01415</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000148">
        <property type="protein sequence ID" value="CCZ83679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01415"/>
    <dbReference type="EnsemblBacteria" id="CCZ83679">
        <property type="protein sequence ID" value="CCZ83679"/>
        <property type="gene ID" value="BN566_01415"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006490">
        <property type="entry name" value="Maj_tail_phi13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006724">
        <property type="entry name" value="Phage_TTP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04630">
        <property type="entry name" value="Phage_TTP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01603">
        <property type="entry name" value="maj_tail_phi13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="198" mass="22042" checksum="FD1F03CD10551312" modified="2013-07-24" version="1">MATETKNKVKFGLNKVYWAKITGYDEDGVPQYAAPVRLPGAVSLSIDANGETEPFYADNCVYYLCNNNSGYEGDLEVALIPTDFATEILGEKLDAKGVLVEKSDAEVSEFALFFEFEGDKKKIRHIFYRCSVARPATESATTEDTKEVKTETLKLSATALDNNLVKSKSCEKTDAETYNNWYNAVYMPSFTAEENKAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>R5V6P1</accession>
    <name>R5V6P1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">2-oxoglutarate ferredoxin oxidoreductase delta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">BN566_01524</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MGS:254</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000162">
        <property type="protein sequence ID" value="CCZ83789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01524"/>
    <dbReference type="EnsemblBacteria" id="CCZ83789">
        <property type="protein sequence ID" value="CCZ83789"/>
        <property type="gene ID" value="BN566_01524"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="2"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="40"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CCZ83789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="68" mass="7633" checksum="85E620E945E23096" modified="2013-07-24" version="1">MEKMKVRFDRCKGCGLCVSVCPKKLVKLQTEKRNEKGYFTAVCTDQDACISCAMCAMMCPDCAIQIEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>R5V7W2</accession>
    <name>R5V7W2_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fructose-1,6-bisphosphatase class 3</fullName>
            <shortName evidence="2">FBPase class 3</shortName>
            <ecNumber evidence="2">3.1.3.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">D-fructose-1,6-bisphosphate 1-phosphohydrolase class 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">fbp</name>
        <name evidence="3" type="ORF">BN566_01934</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>beta-D-fructose 1,6-bisphosphate + H2O = beta-D-fructose 6-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:11064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="EC" id="3.1.3.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FBPase class 3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.11" evidence="2"/>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01934"/>
    <dbReference type="EnsemblBacteria" id="CCZ84209">
        <property type="protein sequence ID" value="CCZ84209"/>
        <property type="gene ID" value="BN566_01934"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042132">
        <property type="term" value="F:fructose 1,6-bisphosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01854">
        <property type="entry name" value="FBPase_class3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009164">
        <property type="entry name" value="FBPtase_class3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06874">
        <property type="entry name" value="FBPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000906">
        <property type="entry name" value="FBPtase_Bacill"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0464">Manganese</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="665" mass="76373" checksum="327118429DC96038" modified="2013-07-24" version="1">MQSTTQASAFEQLQLKYLQRLAEQYPTIDKAAVEIINLQAILNLPKGTEHFITDIHGEYEAFSHVLKNGSGSVKKKIDEVFGHTLTHADKKSLATLIYYPQEKMDIVKKTERHMRDWYKIVLYRLITLCKTLSSKYTRAKVRAAMPQDYAFVMEELITEKPEILDKEAYYETIIDTIIQTDRAESFIVAMSELIQRLAVEHLHILGDIYDRGPAPHKIMDKLLQYHSLDIQWGNHDIVWMGAAAGQLACIATVIRNSARYCNLDILEDGYGINLLPLATFAMAVYQDDPCSQFKLSGNPKTSSTAETALHMKMHKAIAIMQFKLEGQLMKKHPSFEMEDRRLLHLIDYKAGTIEYEGKTLSLLDTHFPTIDPEDPYTLTPEEQLVMERLQSAFLHCEKLQRHVKLLLHHGSLYKVCNGNLLYHGCVPIEEDGTFRKVNIYGNEYSGKALYDCLESYVRKAFLEVDPIEQEKGRDILWYIWTAKNSPLYGKDKMTTFERYFLAEPETHQERKNAYYRFLNDETVMNRILAEFGLGTDSHIVNGHVPVLQIAGESPVKCGGKVMIIDGGFSKVYQEKTGIAGYTLIYHSYGMMLVAHEPFTSTEEAIQKETDIHTDRISVMAASRRLQVRDTDRGREIQERIDELMQLLQAYRSGTLQQEQSFFDNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5V830</accession>
    <name>R5V830_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein-export membrane protein SecG</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">BN566_00028</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in protein export. Participates in an early event of protein translocation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the SecG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000208">
        <property type="protein sequence ID" value="CCZ84289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00028"/>
    <dbReference type="EnsemblBacteria" id="CCZ84289">
        <property type="protein sequence ID" value="CCZ84289"/>
        <property type="gene ID" value="BN566_00028"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015450">
        <property type="term" value="F:protein-transporting ATPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004692">
        <property type="entry name" value="SecG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03840">
        <property type="entry name" value="SecG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00810">
        <property type="entry name" value="secG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="61"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ84289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="85" mass="9434" checksum="46EEEA4D98AE2DEB" modified="2013-07-24" version="1">MGTLEIVIGAVLLLISLVTIIICMMQEQKPQNATAALTGASNDSFYDKNRGRTKEARLHKLTTILTVLFFVLILFIDIVMPLITK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5V8D1</accession>
    <name>R5V8D1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00286</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000222">
        <property type="protein sequence ID" value="CCZ84379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00286"/>
    <dbReference type="EnsemblBacteria" id="CCZ84379">
        <property type="protein sequence ID" value="CCZ84379"/>
        <property type="gene ID" value="BN566_00286"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025945">
        <property type="entry name" value="DHHW"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14286">
        <property type="entry name" value="DHHW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="220"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="439"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="282"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="312"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="384"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="454"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="1038" mass="114416" checksum="6A4AB2FE1E7FC067" modified="2013-07-24" version="1">MRKKNSNSSVPSGMHSLAQRSQQERRQKMEQQRVRTAPQEPMRVPPQQPAHFGRQPMQQVPPPPVQKPEKALSQEALQELQQQATQQPEESAVYNRFFADISVEEPQTPKKSAPQKAAAAVPPTNSAEPEKLAPKMPPVRDARAMPQMPASAKPAAEPQQAEPVQLTPEPPKPVEPQATAESATVSPELLQQVAAMVQQDATGKATLTPELLQKIVAMAQQQTTANPVVSAVSESPAEPQAVPTPEPVAEPAPPQKKSRPARPQRRAPQMPVQAAVPEPVQQPISEPEPQPEPEPTIASPFITTAMLRQMKAEGKTLDDLQPADTKTEKSTPVRRYQGKPAVETAAAPQPEPVRPSFRPVKPQDISIDTAAPEPIPEWFSEEEPPQVAEQRTKYLKNRQPRESVAELTGAAEKKPRAISNGKQPSKAARFFRELFLEDAPVDPEQQKPVLPLTRNTAPPQPEPVATPPQQPIAEPVASPMPESAASVPKQLVMPPVQEAPASSRPTKRPVISHAREEEPEPEPQLESAAFYNRLFNDFPESSEQPVLPTEEEMAQQQHKKDVIESVKGAVSEAEQPGNPPHKKRLTKREYMQRKAQRFSKTIGVSLLSGVVVVLAVVLLLAPRSTISYEENRLLAEKPKFSLTSLLDGSYTSGWSEYYNDTVPFRSKLKKTISAMMQWTGVQSEEDTVFFGNVSQVKKKTTTPVATTESVETTVAVAAGSDETNTEPAVTTTVVTTTEDPNDEPAAEIGEGIILDHKRAVCVYGGSFSVGQDYAETLNAYQQDLGSDVQVYSLVAPTAVSYYLPEEYANYTASETENIDNINSYLNSVKPVDVYNALKPHTAEAIYARTDHHWLPLGAYYAAEAFAKVADVPFASLSDYDTATKKDYVGSMYTYTESAVLLDNPEEFTYYIPKNKYKTTYYSTSFTDPTEGDLLMNLDGYDNSMYYLVFMGGDDKITHVETDCKNGRTLVIFKDSYGNALVPCLTSSFENIYVCDMRYFELNAIDFCKQVGCTDLLFAMNTFSATGGNESYLESNRVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>R5V8P7</accession>
    <name>R5V8P7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_00378</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CBAR010000239">
        <property type="protein sequence ID" value="CCZ84479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00378"/>
    <dbReference type="EnsemblBacteria" id="CCZ84479">
        <property type="protein sequence ID" value="CCZ84479"/>
        <property type="gene ID" value="BN566_00378"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13188">
        <property type="entry name" value="PAS_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="143"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="7">
        <location>
            <begin position="349"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ84479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="574" mass="65081" checksum="9770597B1F048AB8" modified="2013-07-24" version="1">MKKRIFCSMVALVGSAMALCFIFITGLLYEHYSNLQAEQLKETASYIEQGYEAAGQSYLETLQTGTNRVTLIAKDGTVLYDSQEQDVSQMGNHADREEVQQAEQDGVGFSRRFSSTMSKETVYYAVRLSDQSVLRVSVEYRSLFAMMGVLLMQALFILLVMLILALLVSYQLTKKIVKPINTLDLEHPEQVSTYEELDPLLCKLASQNREIVQKMDILRQQQYEFSMITEHMQEGLFVIDHAYTILTCNQSAMRLFGLQHSVLQKNILTVERGEPFRNVLDEALRGRHCTSQLEREGRVYQLIANPVLHNETPQEMQVREDDLIGVVILILDITEKETQERYRREFTANVSHELKTPLTSISGIAEIMKNGIMLPADVPHFAGKIYDESQRLITLIGDIIKLSQLDEQSVPFAWESVNLLELAEDVQKRLQPVCEKRKITFQVTGTPCIVQGVRQVLDEMVYNISENAIKYNREAGSITVTTGYKERHPFIRVTDTGIGIPKAEQERVFERFYRVDKSHSKAVGGTGLGLSIVKHGAILHHATIHLDSTEQVGTTIEILFTGNTEQEEQSEETA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>R5V947</accession>
    <name>R5V947_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptide deformylase</fullName>
            <shortName evidence="2">PDF</shortName>
            <ecNumber evidence="2">3.5.1.88</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Polypeptide deformylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">def</name>
        <name evidence="3" type="ORF">BN566_00482</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>H2O + N-terminal N-formyl-L-methionyl-[peptide] = formate + N-terminal L-methionyl-[peptide]</text>
            <dbReference type="Rhea" id="RHEA:24420"/>
            <dbReference type="Rhea" id="RHEA-COMP:10639"/>
            <dbReference type="Rhea" id="RHEA-COMP:10640"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:49298"/>
            <dbReference type="ChEBI" id="CHEBI:64731"/>
            <dbReference type="EC" id="3.5.1.88"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="2">Binds 1 Fe(2+) ion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the polypeptide deformylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.88" evidence="2"/>
    <dbReference type="EMBL" id="CBAR010000257">
        <property type="protein sequence ID" value="CCZ84629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00482"/>
    <dbReference type="EnsemblBacteria" id="CCZ84629">
        <property type="protein sequence ID" value="CCZ84629"/>
        <property type="gene ID" value="BN566_00482"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042586">
        <property type="term" value="F:peptide deformylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00487">
        <property type="entry name" value="Pep_deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.45.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00163">
        <property type="entry name" value="Pep_deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023635">
        <property type="entry name" value="Peptide_deformylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036821">
        <property type="entry name" value="Peptide_deformylase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10458">
        <property type="entry name" value="PTHR10458"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01327">
        <property type="entry name" value="Pep_deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004749">
        <property type="entry name" value="Pep_def"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01576">
        <property type="entry name" value="PDEFORMYLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56420">
        <property type="entry name" value="SSF56420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00079">
        <property type="entry name" value="pept_deformyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="2">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="156" mass="17723" checksum="45FFAAAE89304828" modified="2013-07-24" version="1">MAIRRIRKEGDPVLRTVCKPVTQFDESLWRLLDDMQETLKDAGGVGLAAPQVGIPKRLFIMDLGLPQMIEAINPQILKVSGKQHVVEGCLSCPDTWGYVDRPKRCRMRAQNRKGEWYEMAFADLGAQCACHENDHLDGHLFVDIVTEFVEPDEEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5VGQ9</accession>
    <name>R5VGQ9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyl transferase group 2 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00571</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000007">
        <property type="protein sequence ID" value="CCZ82766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00571"/>
    <dbReference type="EnsemblBacteria" id="CCZ82766">
        <property type="protein sequence ID" value="CCZ82766"/>
        <property type="gene ID" value="BN566_00571"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_2-like" evidence="1">
        <location>
            <begin position="5"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ82766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="347" mass="39717" checksum="7D1CA4FCA59D3FBA" modified="2013-07-24" version="1">MPTISLCMIVRDEAAVLERCLQSVTHLVDEIIIVDTGSVDETKTIATAFTPHLYDFPWQDDFSAARNFAFSKGTGDYLFWMDADDVLPPESQQQFLERKPYFQADMIVMPYYAALDGQNQPLFTFERERLMRRTANYQWEGAVHEVIPIRGTVQREDIPIWHKKQKPRDADRNLQIYQKQIAAGIPFSPREQYYYARELMEHAAYQAAISAFSKFLADGGGWSVDCATACLCRADCYQQLQQPEAALQSVFQSFQYATPRADACYRIGQYLSAHQQLESAVFWFQLALECGKRPQVGFCQPIYLEELPLTALCQCLLALGKTEEAKPYLKKKELLEKQKGGTLYGML</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>R5VGX2</accession>
    <name>R5VGX2_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Penicillin-binding protein 1A</fullName>
            <ecNumber evidence="8">2.4.1.129</ecNumber>
            <ecNumber evidence="7">3.4.16.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">BN566_00626</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>[GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n)-di-trans,octa-cis-undecaprenyl diphosphate + beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate = [GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-di-trans-octa-cis-undecaprenyl diphosphate + di-trans,octa-cis-undecaprenyl diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23708"/>
            <dbReference type="Rhea" id="RHEA-COMP:9602"/>
            <dbReference type="Rhea" id="RHEA-COMP:9603"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58405"/>
            <dbReference type="ChEBI" id="CHEBI:60033"/>
            <dbReference type="ChEBI" id="CHEBI:78435"/>
            <dbReference type="EC" id="2.4.1.129"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the C-terminal section; belongs to the transpeptidase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">In the N-terminal section; belongs to the glycosyltransferase 51 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.129" evidence="8"/>
    <dbReference type="EC" id="3.4.16.4" evidence="7"/>
    <dbReference type="EMBL" id="CBAR010000011">
        <property type="protein sequence ID" value="CCZ82821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00626"/>
    <dbReference type="EnsemblBacteria" id="CCZ82821">
        <property type="protein sequence ID" value="CCZ82821"/>
        <property type="gene ID" value="BN566_00626"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008658">
        <property type="term" value="F:penicillin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008955">
        <property type="term" value="F:peptidoglycan glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009002">
        <property type="term" value="F:serine-type D-Ala-D-Ala carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3810.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001264">
        <property type="entry name" value="Glyco_trans_51"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036950">
        <property type="entry name" value="PBP_transglycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001460">
        <property type="entry name" value="PCN-bd_Tpept"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00912">
        <property type="entry name" value="Transgly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00905">
        <property type="entry name" value="Transpeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0046">Antibiotic resistance</keyword>
    <keyword evidence="10 22" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="19" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="11" id="KW-0645">Protease</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="12" id="KW-0808">Transferase</keyword>
    <keyword evidence="19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="154"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="Transgly" evidence="21">
        <location>
            <begin position="219"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="Transpeptidase" evidence="20">
        <location>
            <begin position="540"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="875"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="51"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="875"/>
            <end position="932"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00007739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00018638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF00912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="CCZ82821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="953" mass="104095" checksum="0675E12E722175F3" modified="2013-07-24" version="1">MNQESSRNPKHRPAAEKAGKPRAGGTPPKRSSGSRATAASHGKSPAKKPTAARPTGTQNRKPAVKKPTANTTAKRPVSKPPAKPAARTTTANLPAKKPVPPTPPPPDPRERVIYESEADAALCNKPPTGTPEYPYYNDALQAEKPKRFPTLRKAFSILCTTLVSLFLICIITGTIVATGMTVYVMSFRDEVSDVTIEELEMKNNTYVYANDSNGELVCLYKVNNHTERIPVTIDQIPQHVKDAFVSAEDERFYTHDGVDYRRTFYAFVNMFIHIYNTDVGGSTITQQLVKNITGDDVQSPARKIREIFRAMELEQNYSKDEILENYLNYIGFGGPINGVQLASQKYFGKDVSELDIAEAASLAAIPKSPNTLNPFAGYEDEATGEFINTGKEDNKSRQKYVLLQMYDNGSITYDEYQAALNEELIFTDSDEYQETHPEEDNEYLSAEATSWVVDTAIYEFADYLEKTYNLTSNEAIARINAGGYQIATTVDLDMQSYVEDKYKDLDNLVGNKEDVALYLDQNGDGDYSTDEILYPESAFVAMDYEGNIKAIVGSIGEKTESLCWNYATMEPRQPGSTIKPLTTYGLALENDKIHWGSTFTDEPLKQVDGEDWPANYSNSYSHSSVFAYDALAKSLNTVPAKICDDLGTQAVFDFATEKMGLKLDNISTDNESDNDLAPLSLGALTYGVTLENLVSSYIPYGNDGTHFEPHIIKWVKQGEDTLIYENDGSPRKAVSSETAYVMNKLLQNVVENGTGDKAKLQNKHVAGKTGTTENWYDLTFVGMTEDFVSGVWIGYTERQSLPESLESDQIWYNIIGEYADKLDTGASYPSNDDVVEGYVCAKTGKIAGPNCKKLGVGYWKSTNAPVCDGNHYTAPAVTTPSNNNHTTNSSSDESSESSSNTDHSSQGNGGHENSNSSGGDTSQSSGGDSGGGDSGGESSGGESGGESGGEPSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>R5VH42</accession>
    <name>R5VH42_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">4-hydroxy-tetrahydrodipicolinate synthase</fullName>
            <shortName evidence="9">HTPA synthase</shortName>
            <ecNumber evidence="4 9">4.3.3.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">dapA</name>
        <name evidence="11" type="ORF">BN566_00683</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 9">Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>L-aspartate 4-semialdehyde + pyruvate = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:34171"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:67139"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="4.3.3.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 9">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homotetramer; dimer of dimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 10">Belongs to the DapA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Was originally thought to be a dihydrodipicolinate synthase (DHDPS), catalyzing the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to dihydrodipicolinate (DHDP). However, it was shown in E.coli that the product of the enzymatic reaction is not dihydrodipicolinate but in fact (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinic acid (HTPA), and that the consecutive dehydration reaction leading to DHDP is not spontaneous but catalyzed by DapB.</text>
    </comment>
    <dbReference type="EC" id="4.3.3.7" evidence="4 9"/>
    <dbReference type="EMBL" id="CBAR010000021">
        <property type="protein sequence ID" value="CCZ82881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00683"/>
    <dbReference type="EnsemblBacteria" id="CCZ82881">
        <property type="protein sequence ID" value="CCZ82881"/>
        <property type="gene ID" value="BN566_00683"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00017"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008840">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00950">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00418">
        <property type="entry name" value="DapA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005263">
        <property type="entry name" value="DapA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002220">
        <property type="entry name" value="DapA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020625">
        <property type="entry name" value="Schiff_base-form_aldolases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020624">
        <property type="entry name" value="Schiff_base-form_aldolases_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12128">
        <property type="entry name" value="PTHR12128"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00701">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001365">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00146">
        <property type="entry name" value="DHPICSNTHASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01130">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00674">
        <property type="entry name" value="dapA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00665">
        <property type="entry name" value="DHDPS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00666">
        <property type="entry name" value="DHDPS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword evidence="9 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 9" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0704">Schiff base</keyword>
    <feature type="active site" description="Proton donor/acceptor" evidence="9">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="9">
        <location>
            <position position="165"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </ligand>
    </feature>
    <feature type="site" description="Part of a proton relay during catalysis" evidence="9">
        <location>
            <position position="48"/>
        </location>
    </feature>
    <feature type="site" description="Part of a proton relay during catalysis" evidence="9">
        <location>
            <position position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR001365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ82881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="295" mass="31687" checksum="C0EF8C968ADA3FB1" modified="2013-07-24" version="1">MKNTIFTGAGVAIITPMLQDGSVDFEGLKKLIDFQIDNGTDAIIICGTTGESSTLSDEEHRDCIRCAVEHTHNRIPVIAGTGSNDTKYAIELSQEAQELGADGLLLVTPYYNKCTQRGLIAHYTAIADSVDIPIILYNVPSRTGVAIDVATVKALGQHKNIVAVKEASGNIGYTAKLAAQCGDLVDIYSGNDDMIVPIMSLGGKGVISVLSNVMPKETHQMTQYCLENNFAAAAKLQLEYLNLINKLFIEVNPIPVKDALNQMGMPSGPCRMPLYEMSDEHKAALHDALAAHGLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="14" xmlns="http://uniprot.org/uniprot">
    <accession>R5VH56</accession>
    <name>R5VH56_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00693</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000022">
        <property type="protein sequence ID" value="CCZ82891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00693"/>
    <dbReference type="EnsemblBacteria" id="CCZ82891">
        <property type="protein sequence ID" value="CCZ82891"/>
        <property type="gene ID" value="BN566_00693"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="105"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="173"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="69"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="110"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="252"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="318"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ82891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="378" mass="40545" checksum="D644398DF80C80E5" modified="2013-07-24" version="1">MVLDGEQLYETLFVPYSAPRQACLTIDQLSNGTHTLQMTLKSGDFKLDVLEVPENFTMMPDILTVTSAAQAAAEEHALAEQQKALAKKQAAENIKKTLQQAVAALQPEKPAEEPKSETPAAAESETDAEKQPTFKEVLENSGVIGVISNNAAKKKEQLEQDGKTEADETPTVMEFPLPHQDFEAAASLTPIPEHAEESVKIEPLPETDSETSEEPVSAPAAEPESKPEAAAPTESESAAAALVEPEQPAEPQQSEEPISATSEFIATPEEASATETTTSEQPVSEPTAPETATESAAAPSTVTYIEEEWDDLPMPELPPQETASPAPQQPKPTAAPPKKPITPTPSPAPKMPPLHPPVLNDPMQSEPYRKDFNIDIDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>R5VH89</accession>
    <name>R5VH89_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8 9">Xylose isomerase</fullName>
            <ecNumber evidence="2 8 9">5.3.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">xylA</name>
        <name evidence="11" type="ORF">BN566_00716</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 8 9">
            <text>alpha-D-xylose = alpha-D-xylulofuranose</text>
            <dbReference type="Rhea" id="RHEA:22816"/>
            <dbReference type="ChEBI" id="CHEBI:28518"/>
            <dbReference type="ChEBI" id="CHEBI:188998"/>
            <dbReference type="EC" id="5.3.1.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8 10">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 9">Belongs to the xylose isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.5" evidence="2 8 9"/>
    <dbReference type="EMBL" id="CBAR010000027">
        <property type="protein sequence ID" value="CCZ82916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00716"/>
    <dbReference type="EnsemblBacteria" id="CCZ82916">
        <property type="protein sequence ID" value="CCZ82916"/>
        <property type="gene ID" value="BN566_00716"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009045">
        <property type="term" value="F:xylose isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042732">
        <property type="term" value="P:D-xylose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00455">
        <property type="entry name" value="Xylose_isom_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013452">
        <property type="entry name" value="Xylose_isom_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001998">
        <property type="entry name" value="Xylose_isomerase"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00688">
        <property type="entry name" value="XYLOSISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="SSF51658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02630">
        <property type="entry name" value="xylose_isom_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51415">
        <property type="entry name" value="XYLOSE_ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8 9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8 9" id="KW-0859">Xylose metabolism</keyword>
    <feature type="active site" evidence="8">
        <location>
            <position position="100"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="103"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="268"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="271"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="307"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="339"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CCZ82916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="441" mass="49376" checksum="5E40DD2897A2A789" modified="2013-07-24" version="1">MKEYFENIGKIQYEGKESTNPLAFKYYNADEVICGKAMKEQLKFALSWWHTMGGDGTDMFGCGTTDKTWGETDSVKRSISKVDAAFEIMTKLGIEYFCFHDRDLSPEYGSLAETNAKLDEITDYIAEKMKSDPSKKLLWGTAKCFDHPRYMHGAATSPSADVFAYTAAQVKKAVETTVKLGGKGYVFWGGREGYETLLNTNMGLELDNMARLMKMTVDYAHSIGYIGDFYIEPKPKEPTKHQYDFDTATVLGFLRKYGLENDFKMNIEANHATLAQHTFQHELRVARENGVFGSIDANQGDPLLGWDTDQFPTNVYDATFCMYEVLKAGGFTNGGLNFDAKARRGSYTPEDIFYSYIAGMDSFALGLRIAAKIIEDGRIDAFIANRYASWTSGIGKEIIDGKVSMADLEKYALEKGEVTGSLSSGRQEMLESIVNNIMFNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>R5VIE8</accession>
    <name>R5VIE8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01117</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000089">
        <property type="protein sequence ID" value="CCZ83336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01117"/>
    <dbReference type="EnsemblBacteria" id="CCZ83336">
        <property type="protein sequence ID" value="CCZ83336"/>
        <property type="gene ID" value="BN566_01117"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016209">
        <property type="term" value="F:antioxidant activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004407082" description="Thioredoxin domain-containing protein" evidence="2">
        <location>
            <begin position="28"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="3">
        <location>
            <begin position="137"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ83336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="294" mass="31748" checksum="14BC7577B24D3889" modified="2013-07-24" version="1">MKKNWIVTLAACMVFSLSLSGCGTATDTASSTASSESSSEAETSGKSEQDLMDEENNILEKNKDLWEKVFASMDKNVTSDTLSSNYGDVLIAAVENAKDQFSDAEYEMLKADAEQIRKIEDEIAALPTDSSDESTNAAAGSSFPQFEGNDLDGNKVDSSIFAENAVTVVNFWFNECKPCVEELSEMNALNDRIKEQGGEVIGVNVGTLDNNEENIATAKQILETKGAKYRNIYFNSDSDAGKFALGVTAFPTTYVIDRNGNIVGEALLGGINNDDNLNTLQSTIDEVLAKDAQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VIH1</accession>
    <name>R5VIH1_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01132</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000092">
        <property type="protein sequence ID" value="CCZ83356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83356">
        <property type="protein sequence ID" value="CCZ83356"/>
        <property type="gene ID" value="BN566_01132"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="93" mass="11118" checksum="9EE0B1F3EAC46F27" modified="2013-07-24" version="1">MDSFVTNALKYLESHKNFCDNWTEMYKNRVYSPIDEEEKKWMKSRLEEMNQRISIAYDSIISAVYIIPPEWNDITIGVETESEYVFYQWGTSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-09-29" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>R5VII9</accession>
    <name>R5VII9_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Helix-turn-helix</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_01147</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000093">
        <property type="protein sequence ID" value="CCZ83371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83371">
        <property type="protein sequence ID" value="CCZ83371"/>
        <property type="gene ID" value="BN566_01147"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="51"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ83371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="163" mass="18839" checksum="EBA85B4ED4303D36" modified="2013-07-24" version="1">MNDLHTPQISLFSEHEEPQSYEVYVYGTDDLNEQNKDSFCVALCLYLDEINISQKTLARLTGIAPSTLSRYLSGKRKMQYDYLCAVCIAIRLHPFRQRYLFSLLLYAMPCDQDYRKADKNIIRAYLDGCAFDKRFTLTACNEQLKAIHTKPLTNLTSAKEDSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJ62</accession>
    <name>R5VJ62_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ATP synthase alpha/beta family nucleotide-binding domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_01295</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000121">
        <property type="protein sequence ID" value="CCZ83526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83526">
        <property type="protein sequence ID" value="CCZ83526"/>
        <property type="gene ID" value="BN566_01295"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="341" mass="38589" checksum="D3ABA2F88C35E4E1" modified="2013-07-24" version="1">MQPLSFFFDDTIPLRLSEQHPQFLLLSGGTAALKERLLQAVSKAGMQQVQQQFYHANALCGLFFPNLHLVFFDADRFTVLPRLGCPELDTYSISLHDCYSSQILQNHAEAIRYSYAELQENEEHALLLQSAAEQAFSHFCSLTESAVSPEQLPKHPLPRHAVEHGTLELRQLQGCTPEGVLMQPLPADWNTTVLLDPWYTAGSSFLEQLSMEAIQKGYHAILCTHPLQLELPVQLLLPERKQAYILQGSLFGEQFPECDTCSLQDCYPEHTLQAQMVQMQLTAALLQNNMKAYTGMLRVAQGVRTVMQQYYQVAEKPIQIQKQLAQLLCAFHQAEMNHSIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>R5VJY3</accession>
    <name>R5VJY3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Putative septation protein SpoVG</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">spoVG</name>
        <name evidence="5" type="ORF">BN566_01586</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Could be involved in septation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SpoVG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000167">
        <property type="protein sequence ID" value="CCZ83851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83851">
        <property type="protein sequence ID" value="CCZ83851"/>
        <property type="gene ID" value="BN566_01586"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000917">
        <property type="term" value="P:division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030435">
        <property type="term" value="P:sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00819">
        <property type="entry name" value="SpoVG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007170">
        <property type="entry name" value="SpoVG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036751">
        <property type="entry name" value="SpoVG_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38429">
        <property type="entry name" value="PTHR38429"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04026">
        <property type="entry name" value="SpoVG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160537">
        <property type="entry name" value="SSF160537"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1 4" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0717">Septation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CCZ83851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="100" mass="11373" checksum="F21EB5432CD69DAD" modified="2013-07-24" version="1">MEITDIKIRRIITEGRLRAVVSITLDNMLAVHDIKIVQGDERLFVAMPSRKDENGVFRDIVHPISAAARKKLEAEIMETYQQHLALMEAETEAAQHAQHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>R5VKN0</accession>
    <name>R5VKN0_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fe/B12 periplasmic-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_01787</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000184">
        <property type="protein sequence ID" value="CCZ84061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01787"/>
    <dbReference type="EnsemblBacteria" id="CCZ84061">
        <property type="protein sequence ID" value="CCZ84061"/>
        <property type="gene ID" value="BN566_01787"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002491">
        <property type="entry name" value="ABC_transptr_periplasmic_BD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01497">
        <property type="entry name" value="Peripla_BP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50983">
        <property type="entry name" value="FE_B12_PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004395217" description="Fe/B12 periplasmic-binding domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="Fe/B12 periplasmic-binding" evidence="2">
        <location>
            <begin position="93"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="379" mass="42227" checksum="B008A55A9BCC2C32" modified="2013-07-24" version="1">MKWYSLGVSSLLCIGLLTACGSTSSEQTELWSEQSCTGHMTLQYATEFSVDYYDPYALITIADNQQYLLVPEGETVPVGLDTNITVLQQPFQNLYVAASSAMDLFDGIGALEQVRMTSTTEKSWSLPHIQAALEQGTMLYVGKYSAPDYETILSQGCNLAIESTMIYHSPEIQEKLETLGIPVLVERSSYESHPLGRMEWMKLYGLLLNRETEADAYFTAQSEKLDSILTEKNTGKTVAFFYITTNGSVNIRKPGDYTSKMIELAGGNYLFSAEDLNVEENALSTMNIQMETFYAAAKDADYLIYNSTIDGDIPNMEQFLQKSPLLADLKAVKEQHVWCTKQNLFQQTTGAADMIADFHEIVTGAADGKDQLTYLHRIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>R5VKV8</accession>
    <name>R5VKV8_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tyrosine--tRNA ligase</fullName>
            <ecNumber evidence="7">6.1.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Tyrosyl-tRNA synthetase</fullName>
            <shortName evidence="7">TyrRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tyrS</name>
        <name evidence="10" type="ORF">BN566_01886</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + H(+) + L-tyrosyl-tRNA(Tyr)</text>
            <dbReference type="Rhea" id="RHEA:10220"/>
            <dbReference type="Rhea" id="RHEA-COMP:9706"/>
            <dbReference type="Rhea" id="RHEA-COMP:9707"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78536"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.1" evidence="7"/>
    <dbReference type="EMBL" id="CBAR010000193">
        <property type="protein sequence ID" value="CCZ84161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01886"/>
    <dbReference type="EnsemblBacteria" id="CCZ84161">
        <property type="protein sequence ID" value="CCZ84161"/>
        <property type="gene ID" value="BN566_01886"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004831">
        <property type="term" value="F:tyrosine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006437">
        <property type="term" value="P:tyrosyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00805">
        <property type="entry name" value="TyrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02006">
        <property type="entry name" value="Tyr_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002307">
        <property type="entry name" value="Tyr-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024088">
        <property type="entry name" value="Tyr-tRNA-ligase_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024107">
        <property type="entry name" value="Tyr-tRNA-ligase_bac_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11766">
        <property type="entry name" value="PTHR11766"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01040">
        <property type="entry name" value="TRNASYNTHTYR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00234">
        <property type="entry name" value="tyrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 7 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S4" evidence="9">
        <location>
            <begin position="346"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="7">
        <location>
            <begin position="42"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="7">
        <location>
            <begin position="230"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ84161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="408" mass="45830" checksum="75516E2756D5D03D" modified="2013-07-24" version="1">MKNMTVFEELKRRGLIAQMTDEAEIAELINNGKATFYIGFDPTADSLHVGHFMALCLMKRLQMAGNKPIILIGGGTAMIGDPSGKTDMRKMMTKETIQHNVDCFKKQMSRFIDFSEDKAIVVNNGDWLLNLNYIDVLREVGACFSVNRMLTHECYKQRMERGLTFLEFNYMIMQSYDFYKLFQDYGCNMQFGGDDQWANMLGGTELIRRKLGKDAYAMTITLLLNSEGKKMGKTEKGAVWLDAEKTTPYEFYQYWRNVSDQDVIKCLKLLTFVPIEQIEEMERTLKGQQLNQAKELLAYSLTELVHGKEAADQAQDAAKAIFTSGSSSENMPTTAIPVADLQDGGMGILTLMVKAGLCASNGEARRLVQQGGVSVNNEKVTDPKAIITLDGETIIKKGKKVFHKVVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>R5VKZ9</accession>
    <name>R5VKZ9_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phenylacetate-coenzyme A ligase</fullName>
            <ecNumber evidence="1">6.2.1.30</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Phenylacetyl-CoA ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">BN566_01936</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the activation of phenylacetic acid (PA) to phenylacetyl-CoA (PA-CoA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-phenylacetate + ATP + CoA = AMP + diphosphate + phenylacetyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:20956"/>
            <dbReference type="ChEBI" id="CHEBI:18401"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57390"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.30"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Aromatic compound metabolism; phenylacetate degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the phenylacetyl-CoA ligase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.30" evidence="1"/>
    <dbReference type="EMBL" id="CBAR010000194">
        <property type="protein sequence ID" value="CCZ84211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01936"/>
    <dbReference type="EnsemblBacteria" id="CCZ84211">
        <property type="protein sequence ID" value="CCZ84211"/>
        <property type="gene ID" value="BN566_01936"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00930"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047475">
        <property type="term" value="F:phenylacetate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010124">
        <property type="term" value="P:phenylacetate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05913">
        <property type="entry name" value="PaaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028154">
        <property type="entry name" value="AMP-dep_Lig_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011880">
        <property type="entry name" value="PA_CoA_ligase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14535">
        <property type="entry name" value="AMP-binding_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006444">
        <property type="entry name" value="PaaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-binding" evidence="2">
        <location>
            <begin position="82"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding_C_2" evidence="3">
        <location>
            <begin position="337"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR006444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CCZ84211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="436" mass="49192" checksum="8A2579280F0D2261" modified="2013-07-24" version="1">MEQRYYQKEIETMPLEDMKALQSERLVAQVKHVYENVPYYREKMDAAHVKPEDIHGVEDLHKLPFLSKADLRDQYPYGLLAKPLKDCVRIQSTSGTTGRRVVAFYTQHDIDLWEDCCARAVTAVGGTDEDVCQVCYGYGLFTGGAGLNGGSHKVGCLTLPMSSGNTERQIQFMMDLQSTILCCTPSYAAYIGETLKEKGYKPEDNHLKAGIFGAEPWTEEMRQNIQDLLGIRAYDIYGLTETSGPGVAFECCEQHGMHINEDHFIPEIIDPETGEVLPDGEKGELVFTSITKEAFPLLRYRTRDICVLSREKCSCGRTLIKMTKPMGRSDDMMIIRGVNVFPSQIETVLLNEGYSPNYQIEVDRVNNTDTLDVYVELSPEQFTDSLEEITKQEKHLVSAIRTMLGISPKVHLVDPKTIARSEGKAVRVIDKRKLHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="11" xmlns="http://uniprot.org/uniprot">
    <accession>R5VM09</accession>
    <name>R5VM09_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">BN566_00417</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000244">
        <property type="protein sequence ID" value="CCZ84556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84556">
        <property type="protein sequence ID" value="CCZ84556"/>
        <property type="gene ID" value="BN566_00417"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CCZ84556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="142" mass="15955" checksum="6AC021219B507433" modified="2013-07-24" version="1">MGHFKSAESATIAHGIVKPSKSFSIFTGIALVVGIFFLALKRFAKTEDQSPLFVVLAAAFLLTAVFLFLYTKRFQITYDETGFTVRTLFRKPQTYSFADVEAIACKEDSFLLRTKQGNLRLEKELYLGTDAFLAQLQNHVSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>R5VQT3</accession>
    <name>R5VQT3_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ketol-acid reductoisomerase (NADP(+))</fullName>
            <shortName evidence="8">KARI</shortName>
            <ecNumber evidence="8">1.1.1.86</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Acetohydroxy-acid isomeroreductase</fullName>
            <shortName evidence="8">AHIR</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Alpha-keto-beta-hydroxylacyl reductoisomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ilvC</name>
        <name evidence="12" type="ORF">BN566_00722</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(2R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (2S)-2-acetolactate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:22068"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49072"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="1.1.1.86"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP(+) = (S)-2-ethyl-2-hydroxy-3-oxobutanoate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:13493"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49256"/>
            <dbReference type="ChEBI" id="CHEBI:49258"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.86"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8 9">Belongs to the ketol-acid reductoisomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.86" evidence="8"/>
    <dbReference type="EMBL" id="CBAR010000029">
        <property type="protein sequence ID" value="CCZ82922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00722"/>
    <dbReference type="EnsemblBacteria" id="CCZ82922">
        <property type="protein sequence ID" value="CCZ82922"/>
        <property type="gene ID" value="BN566_00722"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00056"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00060"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004455">
        <property type="term" value="F:ketol-acid reductoisomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00435">
        <property type="entry name" value="IlvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013023">
        <property type="entry name" value="KARI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000506">
        <property type="entry name" value="KARI_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013116">
        <property type="entry name" value="KARI_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014359">
        <property type="entry name" value="KARI_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21371">
        <property type="entry name" value="PTHR21371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01450">
        <property type="entry name" value="IlvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07991">
        <property type="entry name" value="IlvN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000116">
        <property type="entry name" value="IlvC_gammaproteo"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00465">
        <property type="entry name" value="ilvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51851">
        <property type="entry name" value="KARI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51850">
        <property type="entry name" value="KARI_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 8 9" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6 8 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0521">NADP</keyword>
    <keyword evidence="8 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KARI N-terminal Rossmann" evidence="10">
        <location>
            <begin position="5"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="KARI C-terminal knotted" evidence="11">
        <location>
            <begin position="189"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="114"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="28"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 9">
        <location>
            <position position="197"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8 9">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 9">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 9">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 9">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CCZ82922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="344" mass="37691" checksum="7D69564AC8E849C0" modified="2013-07-24" version="1">MKQTARVFYQEDCDLSLLYGKTIAVIGYGSQGHAHALNAKESLGDKARVIIGLYEGSKSWAKAEKQGFEVFTTAEAAKQADIIMILINDEKQAAMYKKDIEPNLKSGDMLMFAHGFNIHFGCIKPPADIDVTMIAPKAPGHTVRSEYQAGKGTPCLVAVEQDATGKAMDLALAYGLAIGGARAGILETTFRTETETDLFGEQAVLCGGVCALMQAGFETLCEAGYDPRNAYFECIHEMKLIVDLIYQSGFAGMRYSISNTAEYGDYITGPKIITEDTKKAMKQILKDIQDGSFAKEFLLDMSDAGAQVHFKAMRKLASEHPSEKVGEEIRKLYSWNGEDKLINN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>R5VQU6</accession>
    <name>R5VQU6_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">BN566_00732</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MGS:254</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the auxin efflux carrier (TC 2.A.69) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000029">
        <property type="protein sequence ID" value="CCZ82932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00732"/>
    <dbReference type="EnsemblBacteria" id="CCZ82932">
        <property type="protein sequence ID" value="CCZ82932"/>
        <property type="gene ID" value="BN566_00732"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004776">
        <property type="entry name" value="Mem_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03547">
        <property type="entry name" value="Mem_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="130"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="175"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="205"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="236"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="265"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="291"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CCZ82932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="322" mass="35285" checksum="FC612A32A5B7398A" modified="2013-07-24" version="1">MQTFTNNLIFSLNATIPIFLMMALGFFLQRIHLLDDHTTQKLNQFAFKVLLPALLFMDLSTADFQSVWDTKFVLFCFCITSVSIGIAFLLSLLHRERADRGEFIQAAYRSSAAILGIAFVKNIYGEATMAALMIVGTVPLYNIIAVVVLSLTAPQAEGQPKPNRKALLLKTLKNVATNPIILGIVVGMLWSILRIPQPVILSKSVSYLGNMATPLSLIALGASFQWQDAKGKLPATLGITATKLLLFCALFLPLAVAFGFRTEKLIAVLVMLGSATTGSCFVMARNMGHRGTLTACSVMMTTLCSAFTLTMWLFILRTLQYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2021-06-02" version="10" xmlns="http://uniprot.org/uniprot">
    <accession>R5VR76</accession>
    <name>R5VR76_9FIRM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">BN566_00840</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000042">
        <property type="protein sequence ID" value="CCZ83047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ83047">
        <property type="protein sequence ID" value="CCZ83047"/>
        <property type="gene ID" value="BN566_00840"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CCZ83047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="41" mass="4938" checksum="D033C8C25589D8C0" modified="2013-07-24" version="1">MSGQDVSCQNFNTVFVEYAQNLRYALTFKKKYDTLNLYEFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>R5VRI7</accession>
    <name>R5VRI7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7 8">2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase</fullName>
            <shortName evidence="7">MECDP-synthase</shortName>
            <shortName evidence="7">MECPP-synthase</shortName>
            <shortName evidence="7">MECPS</shortName>
            <ecNumber evidence="3 7 8">4.6.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ispF</name>
        <name evidence="10" type="ORF">BN566_00955</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in the biosynthesis of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), two major building blocks of isoprenoid compounds. Catalyzes the conversion of 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7 8">
            <text>4-CDP-2-C-methyl-D-erythritol 2-phosphate = 2-C-methyl-D-erythritol 2,4-cyclic diphosphate + CMP</text>
            <dbReference type="Rhea" id="RHEA:23864"/>
            <dbReference type="ChEBI" id="CHEBI:57919"/>
            <dbReference type="ChEBI" id="CHEBI:58483"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="4.6.1.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="7">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 4/6.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the IspF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.12" evidence="3 7 8"/>
    <dbReference type="EMBL" id="CBAR010000069">
        <property type="protein sequence ID" value="CCZ83172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_00955"/>
    <dbReference type="EnsemblBacteria" id="CCZ83172">
        <property type="protein sequence ID" value="CCZ83172"/>
        <property type="gene ID" value="BN566_00955"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00056">
        <property type="reaction ID" value="UER00095"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008685">
        <property type="term" value="F:2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019288">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00554">
        <property type="entry name" value="MECDP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00107">
        <property type="entry name" value="IspF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003526">
        <property type="entry name" value="MECDP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020555">
        <property type="entry name" value="MECDP_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036571">
        <property type="entry name" value="MECDP_synthase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43181">
        <property type="entry name" value="PTHR43181"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02542">
        <property type="entry name" value="YgbB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69765">
        <property type="entry name" value="SSF69765"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00151">
        <property type="entry name" value="ispF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01350">
        <property type="entry name" value="ISPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 8" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="6 7 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YgbB" evidence="9">
        <location>
            <begin position="4"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="4-CDP-2-C-methyl-D-erythritol 2-phosphate binding" evidence="7">
        <location>
            <begin position="11"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="region of interest" description="4-CDP-2-C-methyl-D-erythritol 2-phosphate binding" evidence="7">
        <location>
            <begin position="37"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="4-CDP-2-C-methyl-D-erythritol 2-phosphate binding" evidence="7">
        <location>
            <begin position="59"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="4-CDP-2-C-methyl-D-erythritol 2-phosphate binding" evidence="7">
        <location>
            <begin position="64"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="4-CDP-2-C-methyl-D-erythritol 2-phosphate binding" evidence="7">
        <location>
            <begin position="135"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>4-CDP-2-C-methyl-D-erythritol 2-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57919"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="7">
        <location>
            <position position="37"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="7">
        <location>
            <position position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CCZ83172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="161" mass="17334" checksum="C7514712CACF4B62" modified="2013-07-24" version="1">MHKIRIGHGYDVHRLVENRKLILGGVEIPFERGLLGHSDADVLLHAISDALLGAVALGDIGKHFPDTDPRYAGADSMLLLKEVVRLVRNEGYQVGNLDATILCQRPKLAGFISAMRENIAACCEIPVSDVSVKATTEEKLGFTGSGEGIAVHAVALLERID</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>R5VSR7</accession>
    <name>R5VSR7_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Lipoprotein signal peptidase</fullName>
            <ecNumber evidence="6">3.4.23.36</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Prolipoprotein signal peptidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Signal peptidase II</fullName>
            <shortName evidence="6">SPase II</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">lspA</name>
        <name evidence="9" type="ORF">BN566_01340</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MGS:254</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">This protein specifically catalyzes the removal of signal peptides from prolipoproteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains.</text>
            <dbReference type="EC" id="3.4.23.36"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Protein modification; lipoprotein biosynthesis (signal peptide cleavage).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 8">Belongs to the peptidase A8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.23.36" evidence="6"/>
    <dbReference type="EMBL" id="CBAR010000131">
        <property type="protein sequence ID" value="CCZ83572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01340"/>
    <dbReference type="EnsemblBacteria" id="CCZ83572">
        <property type="protein sequence ID" value="CCZ83572"/>
        <property type="gene ID" value="BN566_01340"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00665"/>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00161">
        <property type="entry name" value="LspA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001872">
        <property type="entry name" value="Peptidase_A8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33695">
        <property type="entry name" value="PTHR33695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01252">
        <property type="entry name" value="Peptidase_A8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00781">
        <property type="entry name" value="LIPOSIGPTASE"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00077">
        <property type="entry name" value="lspA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00855">
        <property type="entry name" value="SPASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 7" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="2 6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 6 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 7" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="88"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="126"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CCZ83572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="164" mass="18267" checksum="9918FED82A5C4CD3" modified="2013-07-24" version="1">MILSCICIVLLVGVDQFIKYWAVHTLQPQGSIPFIKIGGKEVLNLTYLENQGAAFSSFSGMRWFLIAVTVLLMVICAVLMVKYRKRSKMIPICSALIIAGGIGNLIDRLFRDGKVIDYLDVRLFHFAVFNFADCCVVVGVFLLAIYMLFLEGSSKKSDRKEGSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>R5VT67</accession>
    <name>R5VT67_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Alpha-galactosidase</fullName>
            <ecNumber evidence="2">3.2.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">BN566_01467</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MGS:254</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.</text>
            <dbReference type="EC" id="3.2.1.22"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.22" evidence="2"/>
    <dbReference type="EMBL" id="CBAR010000159">
        <property type="protein sequence ID" value="CCZ83732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1262953.BN566_01467"/>
    <dbReference type="EnsemblBacteria" id="CCZ83732">
        <property type="protein sequence ID" value="CCZ83732"/>
        <property type="gene ID" value="BN566_01467"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052692">
        <property type="term" value="F:raffinose alpha-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016052">
        <property type="term" value="P:carbohydrate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14791">
        <property type="entry name" value="GH36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038417">
        <property type="entry name" value="Alpga-gal_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000111">
        <property type="entry name" value="Glyco_hydro_27/36_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002252">
        <property type="entry name" value="Glyco_hydro_36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031705">
        <property type="entry name" value="Glyco_hydro_36_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031704">
        <property type="entry name" value="Glyco_hydro_36_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16874">
        <property type="entry name" value="Glyco_hydro_36C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16875">
        <property type="entry name" value="Glyco_hydro_36N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00743">
        <property type="entry name" value="GLHYDRLASE36"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00512">
        <property type="entry name" value="ALPHA_GALACTOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyco_hydro_36N" evidence="6">
        <location>
            <begin position="2"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_hydro_36C" evidence="5">
        <location>
            <begin position="547"/>
            <end position="617"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF16874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF16875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CCZ83732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="625" mass="71028" checksum="67EA1837200C883B" modified="2013-07-24" version="1">MRYQSYSIHKGKYAIAGLPAVYADEDEAETLEIVLKDTATDLQVTLRYGVLPELDMLTRCVSIQNLGTTPITLTKAASFCLDLPYGKWEWMHFHGRHAMERITERTPLIHGIQESSSTRGTSSHHQNPTAILCTPDCTETNGSCFGAAFLYSGSFQTKIEYDQMRQARMVMGLHPDLFRWELQPNATFDTPEVLFTYSDSGLETLSHRFQKTIREHVCRGKYQKIERPVLINNWEATYFDFNEEKILKIAEEAARLGVDMLVLDDGWFGKRDDDCSGLGDWFVNEQKLRGGLGTLVQKVKALGMRFGIWFEPEMISEDSDLYRTHPDWAIQIPGRNPMRSRYQLLLDLSNPDVQDYLYKCISDILNSADISYVKWDMNRSISDWYTALLPANRQGELPHRYVLGVYALLERLTSAFPEVLFEGCSGGGGRFDAGMLYYCPQIWCSDDTDAYERTIIQYGTSFFYPVSAVGSHVSVVPNHQTGRVTPIETRAVTAMAGSFGYELDLNLLSDEEKEAVTQQIQQFKEYGSLIHNGTYYRLSNPLEDAYALWAFVSEDKKEVLVQGMIFRTEANMVRHCVSLRGLDAKAVYRTKDGAAYTGDTLMHAGVLLPKSWGDYYPVSMHFVSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-07-24" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>R5VVR6</accession>
    <name>R5VVR6_9FIRM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SGNH_hydro domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">BN566_00465</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ruminococcus sp. CAG:254</name>
        <dbReference type="NCBI Taxonomy" id="1262953"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Oscillospiraceae</taxon>
            <taxon>Ruminococcus</taxon>
            <taxon>environmental samples</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2012-11" db="EMBL/GenBank/DDBJ databases">
            <title>Dependencies among metagenomic species, viruses, plasmids and units of genetic variation.</title>
            <authorList>
                <person name="Nielsen H.B."/>
                <person name="Almeida M."/>
                <person name="Juncker A.S."/>
                <person name="Rasmussen S."/>
                <person name="Li J."/>
                <person name="Sunagawa S."/>
                <person name="Plichta D."/>
                <person name="Gautier L."/>
                <person name="Le Chatelier E."/>
                <person name="Peletier E."/>
                <person name="Bonde I."/>
                <person name="Nielsen T."/>
                <person name="Manichanh C."/>
                <person name="Arumugam M."/>
                <person name="Batto J."/>
                <person name="Santos M.B.Q.D."/>
                <person name="Blom N."/>
                <person name="Borruel N."/>
                <person name="Burgdorf K.S."/>
                <person name="Boumezbeur F."/>
                <person name="Casellas F."/>
                <person name="Dore J."/>
                <person name="Guarner F."/>
                <person name="Hansen T."/>
                <person name="Hildebrand F."/>
                <person name="Kaas R.S."/>
                <person name="Kennedy S."/>
                <person name="Kristiansen K."/>
                <person name="Kultima J.R."/>
                <person name="Leonard P."/>
                <person name="Levenez F."/>
                <person name="Lund O."/>
                <person name="Moumen B."/>
                <person name="Le Paslier D."/>
                <person name="Pons N."/>
                <person name="Pedersen O."/>
                <person name="Prifti E."/>
                <person name="Qin J."/>
                <person name="Raes J."/>
                <person name="Tap J."/>
                <person name="Tims S."/>
                <person name="Ussery D.W."/>
                <person name="Yamada T."/>
                <person name="MetaHit consortium"/>
                <person name="Renault P."/>
                <person name="Sicheritz-Ponten T."/>
                <person name="Bork P."/>
                <person name="Wang J."/>
                <person name="Brunak S."/>
                <person name="Ehrlich S.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MGS:254</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="CBAR010000257">
        <property type="protein sequence ID" value="CCZ84612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="CCZ84612">
        <property type="protein sequence ID" value="CCZ84612"/>
        <property type="gene ID" value="BN566_00465"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000018181">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013830">
        <property type="entry name" value="SGNH_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13472">
        <property type="entry name" value="Lipase_GDSL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SGNH_hydro" evidence="2">
        <location>
            <begin position="154"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CCZ84612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000018181"/>
        </source>
    </evidence>
    <sequence length="318" mass="33645" checksum="D33B65F466FE9812" modified="2013-07-24" version="1">MDFLKQCPLLICVLLTSLLIPWELLKKEDDSQKAAPTTVAKITTVATEAATTAPPANDGATPDGSETAVSGSDLTTTTVTTEAAGEYVPLANADESYFEDALFIGNSRTVGMYLFGDMPESTDFFADVGMTIYDVMDANIDVPPESGSSNTLTGLLTAKQYGKIYIMFGINELGTGTSESFVEYYQSVVQQICQMQPDAIVYVQSIINVAASAETDVISNANINEKNALLSQIADNKQVFYIDLNPSLVDSSGYLNSDYTSDGIHLGGSSLPLWKDCLLANAMQKNSGAGAARGATTTVATTLDAAAPAEEDSEANPE</sequence>
</entry>
<copyright>
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License
</copyright>
</uniprot>